Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,199,040	G→A	intergenic (+39460/+221897)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,199,040	0	G	A	100.0%	208.2 / NA	77	intergenic (+39460/+221897)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (38/39);  total (38/39)

TTAAGTGCTTGTTCAGAAGAAGTTAAAAGTGTGGAGTATTACAAAGAACATACGACTGAACGTCAAAAAATGTTAGAAGAATGTGAGAAAAAAGCCGAACCGCTGATGGCAAATTCTAATTGTCGAAACGCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGTTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTATTGGATTCAGTTAGTCAATCTATGAGTAGCAATTTTGCTAACTCACTTTTTACAATTATTGGCGTAACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAGATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGCCACAAAAATGATCATTGTAGCATTTATGATGAATTATGGAGGGTATCTGGATAACTCTTTAGCGATAATTGATGATCTCAAA  >  1/1198795‑1199274                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                           ttAAGTGCCTGTTCAGAAGAGGTTAAAAGCGTAGAGTATTACAAAGAACATACGACTGAACGTCAAAAAATGTTAGAAAAATGTGAGAAAAAAGCGGAGCCATTAATGGCAAACTCCAATTGTAGTAATGCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTc                                                                                                                                                                                                                                       <  1:468944/251‑1 (MQ=255)           gTTCAGAAGAGGTTAAAAGCGTAGAGTATTACAAAGAACATACGACTGAACGTCAAAAAATGTTAGAAAAATGTGAGAAAAAAGCGGAGCCATTAATGGCAAACTCCAATTGTAGTAATGCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTc                                                                                                                                                                                                                               <  1:522327/249‑1 (MQ=255)           gTTCAGAAGAGGTTAAAAGCGTAGAGTATTACAAAGAACATACGACTGAACGTCAAAAAATGTTAGAAAAATGTGAGAAAAAAGCGGAGCCATTAATGGCAAACTCCAATTGTAGTAATGCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTc                                                                                                                                                                                                                               >  1:592640/1‑249 (MQ=255)           gTTCAGAAGAGGTTAAAAGCGTAGAGTATTACAAAGAACATACGACTGAACGTCAAAAAATGTTAGAAAAATGTGAGAAAAAAGCGGAGCCATTAATGGCAAACTCCAATTGTAGTAATGCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCaa                                                                                                                                                                                                                             >  1:510211/1‑251 (MQ=255)           gTTCAGAAGAGGTTAAAAGCGTAGAGTATTACAAAGAACATACGACTGAACGTCAAAAAATGTTAGAAAAATGTGAGAAAAAAGCGGAGCCATTAATGGCAAACTCCAATTGTAGTAATGCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCaa                                                                                                                                                                                                                             >  1:755239/1‑251 (MQ=255)           gTTCAGAAGAGGTTAAAAGCGTAGAGTATTACAAAGAACATACGACTGAACGTCAAAAAATGTTAGAAAAATGTGAGAAAAAAGCGGAGCCATTAATGGCAAACTCCAATTGTAGTAATGCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCaa                                                                                                                                                                                                                             >  1:163358/1‑251 (MQ=255)                 aagaggTTAAAAGCGTAGAGTATTACAAAGAACATACGACTGAACGTCAAAAAATGTTAGAAAAATGTGAGAAAAAAGCGGAGCCATTAATGGCAAACTCCAATTGTAGTAATGCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCt                                                                                                                                                                                                                          <  1:616641/248‑1 (MQ=255)                     ggTTAAAAGCGTAGAGTATTACAAAGAACATACGACTGAACGTCAAAAAATGTTAGAAAAATGTGAGAAAAAAGCGGAGCCATTAATGGCAAACTCCAATTGTAGTAATGCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTa                                                                                                                                                                                                                   >  1:617465/2‑251 (MQ=255)                      gTTAAAAGCGTAGAGTATTACAAAGAACATACGACTGAACGTCAAAAAATGTTAGAAAAATGTGAGAAAAAAGCGGAGCCATTAATGGCAAACTCCAATTGTAGTAATGCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGt                                                                                                                                                                                                                    <  1:108910/249‑1 (MQ=255)                         aaaaGCGTAGAGTATTACAAAGAACATACGACTGAACGTCAAAAAATGTTAGAAAAATGTGAGAAAAAAGCGGAGCCATTAATGGCAAACTCCAATTGTAGTAATGCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCag                                                                                                                                                                                                               <  1:148409/251‑2 (MQ=255)                         aaaaGCGTAGAGTATTACAAAGAACATACGACTGAACGTCAAAAAATGTTAGAAAAATGTGAGAAAAAAGCGGAGCCATTAATGGCAAACTCCAATTGTAGTAATGCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCag                                                                                                                                                                                                               <  1:429156/251‑2 (MQ=255)                          aaagcgtagAGTATTACAAAGAACATACGACTGAACGTCAAAAAATGTTAGAAAAATGTGAGAAAAAAGCGGAGCCATTAATGGCAAACTCCAATTGTAGTAATGCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAg                                                                                                                                                                                                                     >  1:514144/9‑244 (MQ=255)                             gcgtagAGTATTACAAAGAACATACGACTGAACGTCAAAAAATGTTAGAAAAATGTGAGAAAAAAGCGGAGCCATTAATGGCAAACTCCAATTGTAGTAATGCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAg                                                                                                                                                                                                                     >  1:685145/6‑241 (MQ=255)                             gcgtagAGTATTACAAAGAACATACGACTGAACGTCAAAAAATGTTAGAAAAATGTGAGAAAAAAGCGGAGCCATTAATGGCAAACTCCAATTGTAGTAATGCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGtttt                                                                                                                                                                                                           >  1:898320/6‑251 (MQ=255)                                  gAGTATTACAAAGAACATACGACTGAACGTCAAAAAATGTTAGAAAAATGTGAGAAAAAAGCGGAGCCATTAATGGCAAACTCCAATTGTAGTAATGCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAg                                                                                                                                                                                                                     >  1:339064/1‑236 (MQ=255)                                    gTATTACAAAGAACATACGACTGAACGTCAAAAAATGTTAGAAAAATGTGAGAAAAAAGCGGAGCCATTAATGGCAAACTCCAATTGTAGTAATGCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTa                                                                                                                                                                                                                         >  1:564909/1‑230 (MQ=255)                                    gTATTACAAAGAACATACGACTGAACGTCAAAAAATGTTAGAAAAATGTGAGAAAAAAGCGGAGCCATTAATGGCAAACTCCAATTGTAGTAATGCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTAt                                                                                                                                                                                                                        <  1:94588/231‑1 (MQ=255)                                      aTTACAAAGAACATACGACTGAACGTCAAAAAATGTTAGAAAAATGTGAGAAAAAAGCGGAGCCATTAATGGCAAACTCCAATTGTAGTAATGCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGt                                                                                                                                                                                                                    >  1:31945/1‑233 (MQ=255)                                              gAACATACGACTGAACGTCAAAAAATGTTAGAAAAATGTGAGAAAAAAGCGGAGCCATTAATGGCAAACTCCAATTGTAGTAATGCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGccagatattacggcaa                                                                                                                                                                                          >  1:593204/1‑236 (MQ=255)                                                aCATACGACTGAACGTCAAAAAATGTTAGAAAAATGTGAGAAAAAAGCGGAGCCATTAATGGCAAACTCCAATTGTAGTAATGCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTc                                                                                                                                                                                                                               >  1:212568/1‑212 (MQ=255)                                                        cTGAACGTCAAAAAATGTTAGAAAAATGTGAGAAAAAAGCGGAGCCATTAATGGCAAACTCCAATTGTAGTAATGCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGc                                                                                                                                                                                <  1:191853/251‑1 (MQ=255)                                                              gTCAAAAAATGTTAGAAAAATGTGAGAAAAAAGCGGAGCCATTAATGGCAAACTCCAATTGTAGTAATGCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAatta                                                                                                                                                                                     <  1:858491/240‑1 (MQ=255)                                                                    aaaTGTTAGAAAAATGTGAGAAAAAAGCGGAGCCATTAATGGCAAACTCCAATTGTAGTAATGCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACa                                                                                                                                                                    <  1:78536/251‑1 (MQ=255)                                                                          tAGAAAAATGTGAGAAAAAAGCGGAGCCATTAATGGCAAACTCCAATTGTAGTAATGCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTAc                                                                                                                                                              <  1:136938/251‑1 (MQ=255)                                                                           agaaaaATGTGAGAAAAAAGCGGAGCCATTAATGGCAAACTCCAATTGTAGTAATGCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGt                                                                                                                                                                                                                                    >  1:657488/1‑180 (MQ=255)                                                                                      agaaaaaagcggagccattaaTGGCAAACTCCAATTGTAGTAATGCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGaaaa                                                                                                                                                  >  1:407200/21‑251 (MQ=255)                                                                                                      attaaTGGCAAACTCCAATTGTAGTAATGCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAatat                                                                                                                                  <  1:6411/247‑1 (MQ=255)                                                                                                       ttaaTGGCAAACTCCAATTGTAGTAATGCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATc                                                                                                                                 <  1:382966/248‑1 (MQ=255)                                                                                                         aaTGGCAAACTCCAATTGTAGTAATGCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTg                                                                                                                               <  1:476506/250‑1 (MQ=255)                                                                                                                  ctccaattgtagtaatgCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGAc                                                                                                                                                                     <  1:39186/188‑1 (MQ=255)                                                                                                                  ctccaattgtagtaatgCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTg                                                                                                                      >  1:159639/17‑251 (MQ=255)                                                                                                                     caattgtagtaatgCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAg                                                                                                                   >  1:112412/14‑251 (MQ=255)                                                                                                                     caattgtagtaatgCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAg                                                                                                                   >  1:175638/14‑251 (MQ=255)                                                                                                                          gtagtaatgCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCa                                                                                                              >  1:660035/9‑251 (MQ=255)                                                                                                                               aatgCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAAc                                                                                                         <  1:583683/248‑1 (MQ=255)                                                                                                                                 tgCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAatat                                                                                                                                  >  1:830391/2‑224 (MQ=255)                                                                                                                                 tgCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAg                                                                                                       >  1:23569/2‑251 (MQ=255)                                                                                                                                       tAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTaa                                                                                                 <  1:16334/251‑1 (MQ=255)                                                                                                                                               aagcaagTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGAt                                                                                         <  1:641640/251‑1 (MQ=255)                                                                                                                                                 gcaagTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGAt                                                                                         <  1:233181/249‑1 (MQ=255)                                                                                                                                                  caagTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGAtttt                                                                                      >  1:225970/1‑251 (MQ=255)                                                                                                                                                  caagTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGAtttt                                                                                      >  1:78949/1‑251 (MQ=255)                                                                                                                                                  caagTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGAtttt                                                                                      >  1:375826/1‑251 (MQ=255)                                                                                                                                                   aagTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCaa                                                                                                                                                                                                                             >  1:754243/1‑115 (MQ=255)                                                                                                                                                     gTATTCGACGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGAt                                                                                                                                              <  1:455285/192‑1 (MQ=255)                                                                                                                                                            aCGTATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCa                                                                                                           >  1:632170/1‑220 (MQ=255)                                                                                                                                                               tATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGa                                                                         >  1:874965/1‑251 (MQ=255)                                                                                                                                                               tATGAATACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGa                                                                         >  1:679291/1‑251 (MQ=255)                                                                                                                                                                   aaTACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGAttat                                                                     <  1:894302/251‑4 (MQ=255)                                                                                                                                                                     tACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTg                                                                   <  1:103477/251‑1 (MQ=255)                                                                                                                                                                     tACTCGAGGTGCTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTg                                                                   >  1:712516/1‑251 (MQ=255)                                                                                                                                                                             gtgctAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTg                                                                                                                                                                                                          >  1:848996/5‑108 (MQ=255)                                                                                                                                                                                  aaTTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTGATTTATGAt                                                                                         >  1:476538/1‑216 (MQ=255)                                                                                                                                                                                         cTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTAt                                               <  1:402433/251‑1 (MQ=255)                                                                                                                                                                                           aaTTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATgg                                             <  1:400285/251‑1 (MQ=255)                                                                                                                                                                                               aGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATgggggg                                         >  1:417582/1‑251 (MQ=255)                                                                                                                                                                                                ggTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATgggg                                           <  1:689464/248‑3 (MQ=255)                                                                                                                                                                                                    aTCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACttt                                                                                                                                                                                              <  1:286516/97‑1 (MQ=255)                                                                                                                                                                                                    aTCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTaa                                                                                                 <  1:894348/190‑1 (MQ=255)                                                                                                                                                                                                       aTTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCtaga                                 <  1:117896/251‑4 (MQ=255)                                                                                                                                                                                                        ttATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGAt                                <  1:142177/251‑1 (MQ=255)                                                                                                                                                                                                        ttATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGAt                                <  1:33248/251‑1 (MQ=255)                                                                                                                                                                                                          aTGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATaa                              >  1:724366/1‑251 (MQ=255)                                                                                                                                                                                                           tGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAAc                             <  1:608627/251‑1 (MQ=255)                                                                                                                                                                                                           tGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAAc                             <  1:546754/251‑1 (MQ=255)                                                                                                                                                                                                               aTTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAActc                           <  1:687334/249‑1 (MQ=255)                                                                                                                                                                                                                  cTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATggggg                                          <  1:882512/231‑4 (MQ=255)                                                                                                                                                                                                                  cTTTGAGAGTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAACTCTTTat                      <  1:868717/251‑2 (MQ=255)                                                                                                                                                                                                                          gTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGAt                                <  1:603979/233‑1 (MQ=255)                                                                                                                                                                                                                          gTATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAACTCTTTATCGATAAtt              >  1:561047/1‑251 (MQ=255)                                                                                                                                                                                                                           tATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAACTCTTTATCGATAAt               <  1:775996/249‑1 (MQ=255)                                                                                                                                                                                                                           tATCGATTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAACTCTTTATCGATAATtg             >  1:383339/1‑251 (MQ=255)                                                                                                                                                                                                                                aTTCTTTCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCt                                    <  1:899676/223‑1 (MQ=255)                                                                                                                                                                                                                                      tCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCtaga                                 >  1:560229/1‑217 (MQ=255)                                                                                                                                                                                                                                      tCGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAACTCTTTATCGATAATTGATGATCTTaaa  >  1:223851/1‑251 (MQ=255)                                                                                                                                                                                                                                       cGTTATTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGa                                                                                          >  1:572285/1‑162 (MQ=255)                                                                                                                                                                                                                                           aTTCAGTTACTAGATTCAGTTAGTCAATCTATGAGTAGCAGTTTTGCCAGCTCACTTTTTACAATTATTGGCATGACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAAATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGTCACAAAAATGATTATTGTGGCATTTATGATGAATTATGGGGGGTATCTAGATAAc                             <  1:230326/219‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                           TTAAGTGCTTGTTCAGAAGAAGTTAAAAGTGTGGAGTATTACAAAGAACATACGACTGAACGTCAAAAAATGTTAGAAGAATGTGAGAAAAAAGCCGAACCGCTGATGGCAAATTCTAATTGTCGAAACGCAAATAAAGCACAAGCAAGTATTCGACGTATGAATACTCGAGGTGTTAATTTTTCTAATTAGGTTATCATTATGAGATTCTTTGAGAGTATCGATTCTTTCGTTATTCAGTTATTGGATTCAGTTAGTCAATCTATGAGTAGCAATTTTGCTAACTCACTTTTTACAATTATTGGCGTAACACTGACAATTTACTTTTTAGGAAAAGGATTTGCCGTAATATCTGGCAAGATTGAAGCTCCAGCAACAGCATTAATTTATGATTTTGCCACAAAAATGATCATTGTAGCATTTATGATGAATTATGGAGGGTATCTGGATAACTCTTTAGCGATAATTGATGATCTCAAA  >  1/1198795‑1199274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑