| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 245,942 | A→C | intergenic (+112631/+16289) | unknown → / ← unknown | tRNA‑Val‑TAC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 245,942 | 0 | A | C | 95.7% | 52.2 / ‑3.2 | 23 | intergenic (+112631/+16289) | unknown/unknown | tRNA‑Val‑TAC/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base C (2/20); minor base G (0/1); total (2/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-01 | |||||||||||
TCCGTAGGGAGGGAAGGCCCGTCTGTGCAAGTGGGCGCGGCGGTGATGTTATGGTGGGGCAATCTTTGTCGTCAATACGGCGTGGCATTCAAAGGACTTAGTGCCAATGAACTGATGGCAACGGGCGCGGCGGGCGGGTTGGCGGCAGCATTTAACGCGCCGTTAGCCGGAGTGATTTTCGCCATTGAAGAACTTGGACGCGGCGTATTATTACGTTGGGAGCGGAGGGTGCTGCTGGGTGTATTGGCGGCGGGTTTCATTTTGGTTGCGATCGAC‑GGCAACAACCCGTATTTTCCTAAATACCACGGCGTCACCGACA‑TTCCTTATTTGTATTTATGGGTTTTGTTGTGTGGCGTGGTTTGCGGGGTGTTCGGCGGCATTTTCGCCCGCCTGCTGGCAAAAGGCGTTGGCGGCGTCTTGCCGAATTTATGCCGTGGCTGGGTGCGCAATCATCCCATTTATAC > 1/245717‑246180 | tCCGTAGGGAGGGAAGGCCCGTCTGTGCAAGTGGGCGCGGCGGTGATGTTATGGTGGGGCAATCTTTGTCGTCAATACGGCGTGGCATTCAAAGGACTTAGTGCCAATGAACTGATGGCAACGGGCGCGGCGGGCGGGGTTGCGGCAGCATTTAACGCGCCGTTAGCCGGGGGGATTTTCGCCCTTGAAGAACTTGGACGCGGCGGATTATTACGTTTGGAGCGGCgggggggggggggggggtgttgggg > 1:658661/1‑229 (MQ=255) tCTGTGCAAGTGGGCGCGGCGGTGATGTTATGGTGGGGCAATCTTTGTCGTCAATACGGCGTGGCATTCAAAGGACTTAGTGCCAATGAACTGATGGCAACGGGCGCGGCGGGCGGGTTGGCGGCAGCATTTAACGCGCCGTTAGCCGGAGTGATTTTCGCCATTGAAGAACTTGGACGCGGCGTATTATTACGTTGGGAGCGGCGGGTGCTGCTGGGTGTATTGGCGGCGGGTTTCATTTTGGTTGCGAt < 1:744123/251‑1 (MQ=255) gTGATGTTATGGTGGGGCAATCTTTGTCGTCAATACGGCGTGGCATTCAAAGGACTTAGTGCCAATGAACTGATGGCAACGGGCGCGGCGGGCGGGTTGGCGGCAGCATTTAACGCGCCGTTAGCCGGAGTGATTTTCGCCATTGAAGAACTTGGACGCGGCGTATTATTACGTTGGGAGCGGCGGGTGCTGCTGGGTGTATTGGCGGCGGGTTTCATTTTGGTTGCGATCGAC‑GGCAACAACCCGTAtt < 1:322149/250‑1 (MQ=255) tGATGTTATGGTGGGGCAATCTTTGTCGTCAATACGGCGTGGCATTCAAAGGACTTAGTGCCAATGAACTGATGGCAACGGGCGCGGCGGGCGGGTTGGCGGCAGCATTTAACGCGCCGTTAGCCGGAGTGATTTTCGCCATTGAAGAACTTGGACGCGGCGTATTATTACGTTGGGAGCGGCGGGTGCTGCTGGGTGTATTGGCGGCGGGTTTCATTTTGGTTGCGATCGAC‑GGCAACAACCCGTAttt < 1:695653/250‑1 (MQ=255) aTGTTATGGTGGGGCAATCTTTGTCGTCAATACGGCGTGGCATTCAAAGGACTTAGTGCCAATGAACTGATGGCAACGGGCGCGGCGGGCGGGTTGGCGGCAGCATTTAACGCGCCGTTAGCCGGAGTGATTTTCGCCATTGAAGAACTTGGACGCGGCGTATTATTACGTTGGGAGCGGCGGGTGCTGCTGGGTGTATTGGCGGCGGGTTTCATTTTGGTTGCGATCGAC‑GGCAACAACCCGTATTTTcc < 1:830310/251‑1 (MQ=255) gtggGGCAATCTTTGTCGTCAATACGGCGTGGCATTCAAAGGACTTAGTGCCAATGAACTGATGGCAACGGGCGCGGCGGGCGGGTTGGCGGCAGCATTTAACGCGCCGTTAGCCGGAGTGATTTTCGCCATTGAAGAACTTGGACGCGGCGTATTATTACGTTGGGAGCGGCGGGTGCTGCTGGGTGTATTGGCGGCGGGTTTCATTTTGGTTGCGATCGAC‑GGCAACAACCCGTATTTTCCTAAATAc < 1:189277/250‑1 (MQ=255) ggCGTGGCATTCAAAGGACTTAGTGCCAATGAACTGATGGCAACGGGCGCGGCGGGCGGGTTGGCGGCAGCATTTAACGCGCCGTTAGCCGGAGTGATTTTCGCCATTGAAGAACTTGGACGCGGCGTATTATTACGTTGGGAGCGGCGGGTGCTGCTGGGTGTATTGGCGGCGGGTTTCATTTTGGTTGCGATCGAC‑GGCAACAACCCGTATTTTCCTAAATACCACGGCGTCACCGAc < 1:643475/240‑1 (MQ=255) aTTCAAAGGACTTAGTGCCAATGAACTGATGGCAACGGGCGCGGCGGGCGGGTTGGCGGCAGCATTTAACGCGCCGTTAGCCGGAGTGATTTTCGCCATTGAAGAACTTGGACGCGGCGTATTATTACGTTGGGAGCGGCGGGTGCTGCTGGGTGTATTGGCGGCGGGTTTCATTTTGGTTGCGATCGAC‑GGCAACAACCCGTATTTTCCTAAATTCCACGGCGTCACCGACA‑TTCCTTATTTGTATTTAt < 1:223447/251‑1 (MQ=255) ccAATGAACTGATGGCAACGGGCGCGGCGGGCGGGTTGGCGGCAGCATTTAACGCGCCGTTAGCCGGAGTGATTTTCGCCATTGAAGAACTTGGACGCGGCGTATTATTACGTTGGGAGCGGCGGGTGCTGCTGGGTGTATTGGCGGCGGGTTTCATTTTGGTTGCGATCGAC‑GGCAACAACCCGTATTTTCCTAAATAc < 1:52806/200‑1 (MQ=255) gggcgggTTGGCGGCAGCATTTAACGCGCCGTTAGCCGGAGTGATTTTCGCCATTGAAGAACTTGGACGCGGCGTATTATTACGTTGGGAGCGGCGGGTGCTGCTGGGTGTATTGGCGGCGGGTTTCATTTTGGTTGCGATCGAC‑GGCAACAACCCGTATTTGCCTAAATGCCACGGCGTCACCGACA‑TTCCTTATTTGTATTTATg < 1:83699/207‑1 (MQ=255) ggTTGGCGGCAGCATTTAACGCGCCGTTAGCCGGAGTGATTTTCGCCATTGAAGAACTTGGACGCGGCGTATTATTACGTTGGGAGCGGCGGGTGCTGCTGGGTGTATTGGCGGCGGGTTTCATTTTGGTTGCGATCGAC‑GGCAACAACCCGTATTTTCCTAAATACCACgg < 1:768985/172‑1 (MQ=255) gcaTTTAACGCGCCGTTAGCCGGAGTGATTTTCGCCATTGAAGAACTTGGACGCGGCGTATTATTACGTTGGGAGCGGCGGGTGCTGCTGGGTGTATTGGCGGCGGGTTTCATTTTGGTTGCGATCGAC‑GGCAACAACCCGTATTTTCCTAAATACCACGGCGTCACCGACA‑TTCCTTATTTGTATTTATGGGTTTTGTTGTGTGGCGTGGTTTGCGGGgt < 1:86773/221‑1 (MQ=255) aTTTAACGCGCCGTTAGCCGGAGTGATTTTCGCCATTGAAGAACTTGGACGCGGCGTATTATTACGTTGGGAGCGGCGGGTGCTGCTGGGTGTATTGGCGGCGGGTTTCATTTTGGTTGCGATCGAC‑GGCAACAACCCGTATTTTCCTAAATACCACGGCGTCACCGACA‑TTCCTTATTTGTATTTATGGGTTttgt < 1:816135/197‑1 (MQ=255) tttAACGCGCCGTTAGCCGGAGTGATTTTCGCCATTGAAGAACTTGGACGCGGCGTATTATTACGTTGGGAGCGGCGGGTGCTGCTGGGGGTATTGGCGGGGGGGTTTTTTTTTGTTGGGGTCGGCGGGCACAAACCCG‑ATTTTTCTTAATAACACGGCGTCCCCCACATTTCTTTTTTTTTTTTTTTGGGTTTT‑TTGTGTGGGGTGGGTTGTGGGGGGGTCGGGGGCGTTTTCTTCCGCCCGCTggggaa > 1:145406/1‑247 (MQ=255) aTTGAAGAACTTGGACGCGGCGTATTATTACGTTGGGAGCGGGGGGTGCTGCTGGGTGTATTGGCGGCGGGTTTCATTTTGGTTGCGATCGAC‑GGCAACAACCCGTATTTTcc < 1:194120/113‑1 (MQ=255) tGAAGAACTTGGACGCGGCGTATTATTACGTTGGGAGCGGCGGGTGCTGCTGGGTGTATTGGCGGCGGGTTTCATTTTGGTTGCGATCGAC‑GGCAACAACCCGTATTTTCCTAAATACCACGGCGTCACCGACA‑TTCCTTATTTGTATTTATGGGTTTTGTTGTGTGGCGTGGtt < 1:111419/175‑1 (MQ=255) gTATTATTACGTTGGGAGCGGCGGGTGCTGCTGGGTGTATTGGCGGCGGGTTTCATTTTGGTTGCGATCGAC‑GGCAGCAACCCGTATTTTCCTAAATACCACGGCGTCACCGACA‑TTCCTTATTTGTATTTATGGGTTttgttg < 1:706191/144‑1 (MQ=255) gTATTATTACGTTGGGAGCGGCGGGTGCTGCTGGGTGTATTGGCGGCGGGTTTCATTTTGGTTGCGATCGAC‑GGCAACAACCCGTAt < 1:398337/87‑1 (MQ=255) gTATTATTACGTTGGGAGCGGCGGGTGCTGCTGGGTGTATTGGCGGCGGGTTTCATTTTGGTTGCGATCGAC‑GGCAACAACCCGTATTTTCCTAAATACCACGGCg < 1:435561/106‑1 (MQ=255) cGTTGGGGGCGGCGGGTGCTGTTGGGTGTGTTGGCGGCGGGTTTCATTTTGGTTGCGATCGAC‑GGCAACAACCCGTATTTTCCTAAATGCCACGGCGTCACCGACA‑TTCCTTAGTTGTATTTATGGGTTTTGTTGTGTGGCGTGGGTTGCGGGGTGTTCGGCGGCATTTTCTCCCGCCTGCTGGCAAAGGGCGTTGGCGGCGTCTTGCCGAATTTATGCCGTGGCTGGGTGCGCAATCATCCCATTTATAc < 1:136478/251‑1 (MQ=255) cGTTGGGAGCGGCGGGTGCTGCTGGGTGTATTGGCGGCGGGTTTCATTTTGGTTGCGATCGAC‑GGCAACAACCCGTATTTTCCTAAATACCACGGCGTCACCGACA‑TTCCTTATTTGTATTTATGGGTTTTGTTGTGTGGCGTGGTTTGCGGGGTGTTCGGCGGCATTTTCTCCCGCCTGCTGGCAAAAGGCGTTGGCGGCGTCTTGCCGAATTTATGCCGTGGCTGGGTGCGCAATCATCCCATTTATAc < 1:659557/251‑1 (MQ=255) cGTTGGGAGCGGCGGGTGCTGCTGGGTGTATTGGCGGCGGGTTTCATTTTGGTTGCGATCGAC‑GGCAACAACCCGTATTTTCCTAAATACCACGGCGTCACCGACA‑TTCCTTATTTGTATTTATGGGTTTTGTTGTGTGGCGTGGTTTGCGGGGTGTTCGGCGGCATTTTCTCCCGCCTGCTGGCAAAAGGCGTTGGCGGCGTCTTGCCGAATTTATGCCGTGGCTGGGTGCGCAATCATCCCATTTATAc < 1:710482/251‑1 (MQ=255) cGTTGGGAGCGGCGGGTGCTGCTGGGTGTATTGGCGGCGGGTTTCATTTTGGTTGCGATCGAC‑GGCAACAACCCGTATTTTCCTAAATACCACGGCGTCACCGACA‑TTCCTTATTTGTATTTATGGGTTTTGTTGTGTGGCGTGGTTTGCGGGGTGTTCGGCGGCATTTTCTCCCGCCTGCTGGCAAAAGGCGTTGGCGGCGTCTTGCCGAATTTATGCCGTGGCTGGGTGCGCAATCATCCCATTTATAc < 1:736777/251‑1 (MQ=255) gcggcgGGTGCTGCTGGGTGTATTGGCGGCGGGTTTCATTTTGGTTGCGATCGAC‑GGCAACAACCCGTATTTTCCTAAATACCACGGCGTCACCGACA‑TTCCTTATTTGTATTTATGGGTTTTGTTGTGTGGCGTGGTTTGCGGGGTGTTCGGCGGCATTTTCTCCCGCCTGCTGGCAAAAGGCGTTGGCGGCGTCTTGCCGAATTTATGCCGTGGCTGGGTGCGCAATCATCCCATTTATAc > 1:217392/1‑243 (MQ=255) | TCCGTAGGGAGGGAAGGCCCGTCTGTGCAAGTGGGCGCGGCGGTGATGTTATGGTGGGGCAATCTTTGTCGTCAATACGGCGTGGCATTCAAAGGACTTAGTGCCAATGAACTGATGGCAACGGGCGCGGCGGGCGGGTTGGCGGCAGCATTTAACGCGCCGTTAGCCGGAGTGATTTTCGCCATTGAAGAACTTGGACGCGGCGTATTATTACGTTGGGAGCGGAGGGTGCTGCTGGGTGTATTGGCGGCGGGTTTCATTTTGGTTGCGATCGAC‑GGCAACAACCCGTATTTTCCTAAATACCACGGCGTCACCGACA‑TTCCTTATTTGTATTTATGGGTTTTGTTGTGTGGCGTGGTTTGCGGGGTGTTCGGCGGCATTTTCGCCCGCCTGCTGGCAAAAGGCGTTGGCGGCGTCTTGCCGAATTTATGCCGTGGCTGGGTGCGCAATCATCCCATTTATAC > 1/245717‑246180 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |