Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,198,433	T→C	intergenic (+38853/+222504)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,198,433	0	T	C	100.0%	209.3 / NA	73	intergenic (+38853/+222504)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (33/40);  total (33/40)

CTGAAAGCGTATGCCGACCAATTAGCCTCACAGACAGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTGTATGGTGAAATAAATGGCTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCTAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAGAATTTACGTCAAGGTGAAATTACTTCCTGTGAAGCAAATATTGTTTCCACCGTGGAAAGTATGAGCTTCTTAGATTCGTTTGAAAGTAATGTCAATAGCAAACTTAAAGCAATTGATAAGTTAGCTAATCGTATGGCGAAATCAAACGATGTAAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGATTTATTCAACGCACGGCAAACGGAATACCGACGCTTAATG  >  1/1198203‑1198679                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       ctcaaggCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAatat                                                                                                                                                                                                                                    >  1:358342/7‑251 (MQ=255)   caaggCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTg                                                                                                                                                                                                                                  <  1:656982/247‑1 (MQ=255)      ggCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGttt                                                                                                                                                                                                                               >  1:639592/2‑251 (MQ=255)      ggCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGttt                                                                                                                                                                                                                               >  1:234735/2‑251 (MQ=255)       gCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGttt                                                                                                                                                                                                                               >  1:515667/1‑250 (MQ=255)       gCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGttt                                                                                                                                                                                                                               <  1:42943/250‑1 (MQ=255)        cGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTcc                                                                                                                                                                                                                             <  1:92052/251‑1 (MQ=255)          tATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCAc                                                                                                                                                                                                                           <  1:854013/251‑1 (MQ=255)                 aCCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGag                                                                                                                                                                                                                    <  1:728930/251‑2 (MQ=255)                 aCCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGag                                                                                                                                                                                                                    <  1:724952/251‑2 (MQ=255)                  ccAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCAc                                                                                                                                                                                                                           <  1:816596/243‑1 (MQ=255)                   cAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGagag                                                                                                                                                                                                                  >  1:350774/1‑248 (MQ=255)                   cAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGagag                                                                                                                                                                                                                  >  1:59247/1‑248 (MQ=255)                       tAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATg                                                                                                                                                                                                              <  1:495735/251‑1 (MQ=255)                          ccTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGCGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGc                                                                                                                                                                                                           <  1:32216/251‑1 (MQ=255)                           cTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGct                                                                                                                                                                                                          <  1:306939/251‑1 (MQ=255)                           cTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGct                                                                                                                                                                                                          <  1:603477/251‑1 (MQ=255)                           cTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGCGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGct                                                                                                                                                                                                          <  1:325610/251‑1 (MQ=255)                                 gaccggAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGag                                                                                                                                                                                                                    <  1:830797/231‑2 (MQ=255)                                    cggAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGAtt                                                                                                                                                                                                 <  1:632917/250‑1 (MQ=255)                                            gTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGaa                                                                                                                                                                                         >  1:317149/1‑251 (MQ=255)                                                 gTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAg                                                                                                                                                                                                            <  1:895558/227‑1 (MQ=255)                                                 gTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTaa                                                                                                                                                                                    >  1:548180/1‑251 (MQ=255)                                                 gTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTaa                                                                                                                                                                                    >  1:412725/1‑251 (MQ=255)                                                      aGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCa                                                                                                                                                                               >  1:600379/1‑251 (MQ=255)                                                       gCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGATGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCaa                                                                                                                                                                              >  1:441807/1‑251 (MQ=255)                                                                      gcgcATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGag                                                                                                                                                                                                                    <  1:427095/198‑2 (MQ=255)                                                                       cgcATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTaaattgaaaac                                                                                                                                                               <  1:719511/250‑9 (MQ=255)                                                                        gcATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTaacg                                                                                                                                                                                  <  1:487590/230‑3 (MQ=255)                                                                        gcATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTaaattgaaaacta                                                                                                                                                             >  1:757994/1‑241 (MQ=255)                                                                           tAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGAtt                                                                                                                                                                                                 >  1:34871/1‑212 (MQ=255)                                                                           tAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTaaattgaaaactattg                                                                                                                                                          >  1:850073/1‑238 (MQ=255)                                                                           tAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTaaattgaaaactattg                                                                                                                                                          >  1:329674/1‑238 (MQ=255)                                                                            aGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATAGTTTCCACCGTGGAGAGTATGAGCTTCTTAGAtt                                                                                                                                                                                                 <  1:357477/211‑1 (MQ=255)                                                                             gTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGa                                                                                                                                                                                                                                             >  1:674913/1‑166 (MQ=255)                                                                             gTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCCAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTaaattgaaaactattgat                                                                                                                                                        >  1:426661/1‑236 (MQ=255)                                                                             gTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGt                                                                                                                                                                                                                 <  1:587004/194‑1 (MQ=255)                                                                             gTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGct                                                                                                                                                                                                          >  1:372749/1‑201 (MQ=255)                                                                             gTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAgt                                                                                                                                                                          <  1:675182/233‑2 (MQ=255)                                                                             gTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTaaattgaaaactattgat                                                                                                                                                        >  1:299863/1‑236 (MQ=255)                                                                             gTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATCTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTaaattgaaaactattgat                                                                                                                                                        >  1:812671/1‑236 (MQ=255)                                                                             gTTTATATGGCGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCAc                                                                                                                                                                                                                           >  1:86324/1‑184 (MQ=255)                                                                                 atATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGAtt                                                                                                                                                                                                 >  1:254252/2‑206 (MQ=255)                                                                                     ggTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATaaactcg                                                                                                                                                 >  1:690135/1‑245 (MQ=255)                                                                                      gTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTaaattga                                                                                                                                                                   >  1:96845/1‑227 (MQ=255)                                                                                        gAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGcttctt                                                                                                                                                                                                      >  1:665406/1‑194 (MQ=255)                                                                                        gAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAg                                                                                                                                                                                       >  1:263973/1‑209 (MQ=255)                                                                                           ataaatGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAg                                                                                                                                                                           >  1:211021/1‑218 (MQ=255)                                                                                           ataaatGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATaaactcgctaaacg                                                                                                                                          <  1:673838/251‑13 (MQ=255)                                                                                                                                aTTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGa                                                                                                      >  1:58629/1‑250 (MQ=255)                                                                                                                                      tttCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTaaaa                                                                                               <  1:805680/251‑1 (MQ=255)                                                                                                                                            gTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGc                                                                                          <  1:482867/250‑1 (MQ=255)                                                                                                                                                      cGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTaaattgaaaac                                                                                                                                                               >  1:607727/1‑163 (MQ=255)                                                                                                                                                        cATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCATTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAg                                                                             <  1:447650/251‑1 (MQ=255)                                                                                                                                                           tAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTg                                                                          <  1:671825/251‑1 (MQ=255)                                                                                                                                                               cgcgAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCg                                                                      >  1:746470/1‑251 (MQ=255)                                                                                                                                                                 cgAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGc                                                                     <  1:405385/250‑1 (MQ=255)                                                                                                                                                                     tgccaaaaGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGc                                                                <  1:827840/247‑1 (MQ=255)                                                                                                                                                                      gccaaaaGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTct                                                                                                                                                                                                                                                  >  1:679439/4‑71 (MQ=255)                                                                                                                                                                               cTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACaaaa                                                      <  1:880963/251‑1 (MQ=255)                                                                                                                                                                                     gAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAg                                                                                                                                                                           <  1:451876/128‑1 (MQ=255)                                                                                                                                                                                            aCAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGAt                                         <  1:49409/251‑1 (MQ=255)                                                                                                                                                                                              aaCCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGAttt                                       <  1:252915/251‑1 (MQ=255)                                                                                                                                                                                                 cTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAg                                                    >  1:269481/1‑235 (MQ=255)                                                                                                                                                                                                  tttttGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGATTTATTc                                   <  1:190311/251‑1 (MQ=255)                                                                                                                                                                                                   ttttGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGAc                                                                                                     <  1:523565/184‑1 (MQ=255)                                                                                                                                                                                                                aaaaaTTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGATTTATTCAACGCACGGCAAAc                     <  1:645212/248‑1 (MQ=255)                                                                                                                                                                                                                           tCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGATTTATTCAACGCACGGCAAACGGAATAccta           <  1:506760/250‑3 (MQ=255)                                                                                                                                                                                                                             aaGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGATTTATTCAACGCACGGCAAACGGAATACCTACGc        <  1:772400/251‑1 (MQ=255)                                                                                                                                                                                                                             aaGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGATTTATTCAACGCACGGCAAACGGAATACCTACGc        <  1:18114/251‑1 (MQ=255)                                                                                                                                                                                                                                gTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGATTTATTCAACGCACGGCAAACGGAATACCTACGCt       >  1:794316/1‑249 (MQ=255)                                                                                                                                                                                                                                   aaaTCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGATTTATTCAACGCACGGCAAACGGAATACCTACGCTTaatg  <  1:176915/251‑1 (MQ=255)                                                                                                                                                                                                                                   aaaTCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGATTTATTCAACGCACGGCAAACGGAATACCTACGCTTaat   <  1:619408/250‑1 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       CTGAAAGCGTATGCCGACCAATTAGCCTCACAGACAGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTGTATGGTGAAATAAATGGCTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCTAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAGAATTTACGTCAAGGTGAAATTACTTCCTGTGAAGCAAATATTGTTTCCACCGTGGAAAGTATGAGCTTCTTAGATTCGTTTGAAAGTAATGTCAATAGCAAACTTAAAGCAATTGATAAGTTAGCTAATCGTATGGCGAAATCAAACGATGTAAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGATTTATTCAACGCACGGCAAACGGAATACCGACGCTTAATG  >  1/1198203‑1198679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑