Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,198,418	A→G	intergenic (+38838/+222519)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,198,418	0	A	G	100.0%	203.6 / NA	72	intergenic (+38838/+222519)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/40);  total (32/40)

TTTCGCATTATAAAAATGAGCTGAAAGCGTATGCCGACCAATTAGCCTCACAGACAGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTGTATGGTGAAATAAATGGCTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCTAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAGAATTTACGTCAAGGTGAAATTACTTCCTGTGAAGCAAATATTGTTTCCACCGTGGAAAGTATGAGCTTCTTAGATTCGTTTGAAAGTAATGTCAATAGCAAACTTAAAGCAATTGATAAGTTAGCTAATCGTATGGCGAAATCAAACGATGTAAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGATTTATTCAACGCACGGCAAAC  >  1/1198183‑1198660                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                   tatctcATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAAtc                                                                                                                                                                                                                                     <  1:350373/246‑2 (MQ=255) tatctcATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAAtc                                                                                                                                                                                                                                     <  1:239945/246‑2 (MQ=255)   tctcATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAAtcac                                                                                                                                                                                                                                   <  1:142724/248‑4 (MQ=255)                     ctcaaggCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAatat                                                                                                                                                                                                                 >  1:358342/7‑251 (MQ=255)                       caaggCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTg                                                                                                                                                                                                               <  1:656982/247‑1 (MQ=255)                          ggCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGttt                                                                                                                                                                                                            >  1:234735/2‑251 (MQ=255)                          ggCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGttt                                                                                                                                                                                                            >  1:639592/2‑251 (MQ=255)                           gCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGttt                                                                                                                                                                                                            <  1:42943/250‑1 (MQ=255)                           gCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGttt                                                                                                                                                                                                            >  1:515667/1‑250 (MQ=255)                            cGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTcc                                                                                                                                                                                                          <  1:92052/251‑1 (MQ=255)                              tATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCAc                                                                                                                                                                                                        <  1:854013/251‑1 (MQ=255)                                     aCCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGag                                                                                                                                                                                                 <  1:728930/251‑2 (MQ=255)                                     aCCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGag                                                                                                                                                                                                 <  1:724952/251‑2 (MQ=255)                                      ccAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCAc                                                                                                                                                                                                        <  1:816596/243‑1 (MQ=255)                                       cAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGagag                                                                                                                                                                                               >  1:59247/1‑248 (MQ=255)                                       cAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGagag                                                                                                                                                                                               >  1:350774/1‑248 (MQ=255)                                           tAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATg                                                                                                                                                                                           <  1:495735/251‑1 (MQ=255)                                              ccTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGCGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGc                                                                                                                                                                                        <  1:32216/251‑1 (MQ=255)                                               cTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGct                                                                                                                                                                                       <  1:603477/251‑1 (MQ=255)                                               cTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGct                                                                                                                                                                                       <  1:306939/251‑1 (MQ=255)                                               cTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGCGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGct                                                                                                                                                                                       <  1:325610/251‑1 (MQ=255)                                                     gaccggAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGag                                                                                                                                                                                                 <  1:830797/231‑2 (MQ=255)                                                        cggAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGAtt                                                                                                                                                                              <  1:632917/250‑1 (MQ=255)                                                                gTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGaa                                                                                                                                                                      >  1:317149/1‑251 (MQ=255)                                                                     gTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAg                                                                                                                                                                                         <  1:895558/227‑1 (MQ=255)                                                                     gTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTaa                                                                                                                                                                 >  1:548180/1‑251 (MQ=255)                                                                     gTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTaa                                                                                                                                                                 >  1:412725/1‑251 (MQ=255)                                                                          aGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCa                                                                                                                                                            >  1:600379/1‑251 (MQ=255)                                                                           gCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGATGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCaa                                                                                                                                                           >  1:441807/1‑251 (MQ=255)                                                                                          gcgcATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGag                                                                                                                                                                                                 <  1:427095/198‑2 (MQ=255)                                                                                           cgcATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTaaattgaaaac                                                                                                                                            <  1:719511/250‑9 (MQ=255)                                                                                            gcATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTaacg                                                                                                                                                               <  1:487590/230‑3 (MQ=255)                                                                                            gcATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTaaattgaaaacta                                                                                                                                          >  1:757994/1‑241 (MQ=255)                                                                                               tAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGAtt                                                                                                                                                                              >  1:34871/1‑212 (MQ=255)                                                                                               tAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTaaattgaaaactattg                                                                                                                                       >  1:329674/1‑238 (MQ=255)                                                                                               tAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTaaattgaaaactattg                                                                                                                                       >  1:850073/1‑238 (MQ=255)                                                                                                aGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATAGTTTCCACCGTGGAGAGTATGAGCTTCTTAGAtt                                                                                                                                                                              <  1:357477/211‑1 (MQ=255)                                                                                                 gTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAAtc                                                                                                                                                                                                                                     <  1:760279/155‑2 (MQ=255)                                                                                                 gTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGa                                                                                                                                                                                                                          >  1:674913/1‑166 (MQ=255)                                                                                                 gTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCCAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTaaattgaaaactattgat                                                                                                                                     >  1:426661/1‑236 (MQ=255)                                                                                                 gTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGt                                                                                                                                                                                              <  1:587004/194‑1 (MQ=255)                                                                                                 gTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGct                                                                                                                                                                                       >  1:372749/1‑201 (MQ=255)                                                                                                 gTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAgt                                                                                                                                                       <  1:675182/233‑2 (MQ=255)                                                                                                 gTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTaaattgaaaactattgat                                                                                                                                     >  1:299863/1‑236 (MQ=255)                                                                                                 gTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATCTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTaaattgaaaactattgat                                                                                                                                     >  1:812671/1‑236 (MQ=255)                                                                                                 gTTTATATGGCGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCAc                                                                                                                                                                                                        >  1:86324/1‑184 (MQ=255)                                                                                                     atATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGAtt                                                                                                                                                                              >  1:254252/2‑206 (MQ=255)                                                                                                         ggTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATaaactcg                                                                                                                              >  1:690135/1‑245 (MQ=255)                                                                                                          gTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTaaattga                                                                                                                                                >  1:96845/1‑227 (MQ=255)                                                                                                            gAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGcttctt                                                                                                                                                                                   >  1:665406/1‑194 (MQ=255)                                                                                                            gAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAg                                                                                                                                                                    >  1:263973/1‑209 (MQ=255)                                                                                                             aaataaatGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAAtc                                                                                                                                                                                                                                     <  1:509456/143‑2 (MQ=255)                                                                                                               ataaatGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAg                                                                                                                                                        >  1:211021/1‑218 (MQ=255)                                                                                                               ataaatGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATaaactcgctaaacg                                                                                                                       <  1:673838/251‑13 (MQ=255)                                                                                                                                                    aTTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGa                                                                                   >  1:58629/1‑250 (MQ=255)                                                                                                                                                          tttCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTaaaa                                                                            <  1:805680/251‑1 (MQ=255)                                                                                                                                                                gTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGc                                                                       <  1:482867/250‑1 (MQ=255)                                                                                                                                                                          cGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTaaattgaaaac                                                                                                                                            >  1:607727/1‑163 (MQ=255)                                                                                                                                                                            cATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCATTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAg                                                          <  1:447650/251‑1 (MQ=255)                                                                                                                                                                               tAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTg                                                       <  1:671825/251‑1 (MQ=255)                                                                                                                                                                                   cgcgAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCg                                                   >  1:746470/1‑251 (MQ=255)                                                                                                                                                                                     cgAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGc                                                  <  1:405385/250‑1 (MQ=255)                                                                                                                                                                                         tgccaaaaGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGc                                             <  1:827840/247‑1 (MQ=255)                                                                                                                                                                                          gccaaaaGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTct                                                                                                                                                                                                                               >  1:679439/4‑71 (MQ=255)                                                                                                                                                                                                   cTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACaaaa                                   <  1:880963/251‑1 (MQ=255)                                                                                                                                                                                                         gAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAg                                                                                                                                                        <  1:451876/128‑1 (MQ=255)                                                                                                                                                                                                                aCAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGAt                      <  1:49409/251‑1 (MQ=255)                                                                                                                                                                                                                  aaCCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGAttt                    <  1:252915/251‑1 (MQ=255)                                                                                                                                                                                                                     cTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAg                                 >  1:269481/1‑235 (MQ=255)                                                                                                                                                                                                                      tttttGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGATTTATTc                <  1:190311/251‑1 (MQ=255)                                                                                                                                                                                                                       ttttGACCGTTGTAAAAATTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGAc                                                                                  <  1:523565/184‑1 (MQ=255)                                                                                                                                                                                                                                    aaaaaTTTGCGTCAAGGTGAAATCACTTCTTGTGAAGCAAATATTGTTTCCACCGTGGAGAGTATGAGCTTCTTAGATTCGTTTGAAAGTAACGTCAATAGTAAATTGAAAACTATTGATAAACTCGCTAAACGGATGACAAAATCTCAAGATGTCAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGATTTATTCAACGCACGGCAAAc  <  1:645212/248‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                   TTTCGCATTATAAAAATGAGCTGAAAGCGTATGCCGACCAATTAGCCTCACAGACAGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTGTATGGTGAAATAAATGGCTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCTAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAGAATTTACGTCAAGGTGAAATTACTTCCTGTGAAGCAAATATTGTTTCCACCGTGGAAAGTATGAGCTTCTTAGATTCGTTTGAAAGTAATGTCAATAGCAAACTTAAAGCAATTGATAAGTTAGCTAATCGTATGGCGAAATCAAACGATGTAAAAGAAAGCCAGGACTTAAAAAATGCAATGGACGTGCAGTTGGCCGCATTGCAATCACAAAAAGTCCAGTTGGATTTATTCAACGCACGGCAAAC  >  1/1198183‑1198660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑