Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,198,199	T→C	intergenic (+38619/+222738)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,198,199	0	T	C	100.0%	166.7 / NA	62	intergenic (+38619/+222738)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/28);  total (34/28)

ATTTATAAACAACGTTGTGTATAAGGAGAACGTGATGAAAATGTTTAAAAAACTCACTTTAATTGCTGTTTTATCTATGCCGGTATTGGTACAAGCCAGCGGTATTCCGGTTGTTGATGCCGCAGCTGCCGGTCAACGTCAAATGAGCATGCTTCAAACGGTGCAACAATGGGCAAAAGAGGCTAAACAGTGGACGGATACAGTTTCGCATTATAAAAATGAGCTGAAAGCGTATGCCGACCAATTAGCCTCACAGACAGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTGTATGGTGAAATAAATGGCTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCTAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAGAATTTACGTCAAGGTGAAATTAC  >  1/1197980‑1198435                                                                                                                                                                                                                            |                                                                                                                                                                                                                                             aTTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGc                                                                                                                                                                                                                             <  1:237594/237‑1 (MQ=255)  tttATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCt                                                                                                                                                                                                               >  1:567908/1‑250 (MQ=255)            acGTTGTGTATAAGGAGAACGTGATGAAAATGTTTAAAAAACTCACTTTAATTGCTGTCTTGTCCATACCGGCATTAGTTCAAGCCAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGcc                                                                                                                                                                                                                >  1:679189/1‑239 (MQ=255)            acGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGa                                                                                                                                                                                                    >  1:364165/1‑251 (MQ=255)              gTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAAt                                                                                                                                                                                                  >  1:799367/1‑251 (MQ=255)               ttGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAAt                                                                                                                                                                                                  <  1:342920/250‑1 (MQ=255)                 gtgtATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCacag                                                                                                                                                                                                          <  1:336927/240‑1 (MQ=255)                 gtgtATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCg                                                                                                                                                                                               >  1:58906/1‑251 (MQ=255)                  tgtATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGt                                                                                                                                                                                              >  1:337627/1‑251 (MQ=255)                   gtATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTg                                                                                                                                                                                             >  1:691574/1‑251 (MQ=255)                   gtATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTg                                                                                                                                                                                             >  1:294045/1‑251 (MQ=255)                   gtATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTg                                                                                                                                                                                             >  1:725014/1‑251 (MQ=255)                       aaGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTg                                                                                                                                                                                             >  1:142079/1‑247 (MQ=255)                              aCGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAACACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGc                                                                                                                                                                                   >  1:592353/1‑250 (MQ=255)                                      aaaaTCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTgg                                                                                                                                                                          <  1:828561/251‑1 (MQ=255)                                              tAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAgcgc                                                                                                                                                                  <  1:655244/251‑1 (MQ=255)                                                        aCTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGtttata                                                                                                                                                        <  1:735545/251‑4 (MQ=255)                                                         cTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTatat                                                                                                                                                       >  1:260477/1‑251 (MQ=255)                                                         cTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTatat                                                                                                                                                       >  1:490461/1‑251 (MQ=255)                                                                    gtcttgtCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCacagacc                                                                                                                                                                                                       <  1:872881/186‑2 (MQ=255)                                                                    gtcttgtCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAAtt                                                                                                                                                                                                 <  1:177883/192‑1 (MQ=255)                                                                        tgtCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATggt                                                                                                                                        >  1:25655/3‑250 (MQ=255)                                                                               accggcattagttcAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAAt                                                                                                                                 <  1:687568/238‑1 (MQ=255)                                                                               accggcattagttcAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAAt                                                                                                                                 <  1:244693/238‑1 (MQ=255)                                                                                   gcattagttcAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGtttata                                                                                                                                                        >  1:809715/10‑221 (MQ=255)                                                                                         gttcAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGtttata                                                                                                                                                        >  1:809530/4‑215 (MQ=255)                                                                                           tcAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTAccc                                                                                                                     <  1:245680/250‑1 (MQ=255)                                                                                              agctaGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCacagacc                                                                                                                                                                                                       <  1:463962/162‑2 (MQ=255)                                                                                                      gTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGAt                                                                                                          >  1:110855/1‑251 (MQ=255)                                                                                                         ttCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTa                                                                                                       >  1:803527/1‑251 (MQ=255)                                                                                                           ccGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACGAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGt                                                                                                     >  1:447852/1‑251 (MQ=255)                                                                                                              gtagttgatggtgctgCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAAt                                                                                                                                 <  1:620605/205‑1 (MQ=255)                                                                                                                agttgatggtgctgCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGaaat                                                                                                                                               <  1:695142/191‑1 (MQ=255)                                                                                                                          gctgCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGGAGCGCATAGTTTATATg                                                                                                                                                      >  1:435093/4‑187 (MQ=255)                                                                                                                                     tCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAgcg                                                                           <  1:703525/251‑1 (MQ=255)                                                                                                                                              aatcagtatgttacAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAg                                                                                                                                                                     >  1:114637/14‑152 (MQ=255)                                                                                                                                              aatcagtatgttacAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGcca                                                                  >  1:889212/14‑249 (MQ=255)                                                                                                                                                tcagtatgttacAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGaaataa                                                                                                                                             >  1:341936/12‑174 (MQ=255)                                                                                                                                                   gtatgttacAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGcc                                                                                                                                                                                  <  1:66591/126‑1 (MQ=255)                                                                                                                                                   gtatgttacAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGttt                                                                                                                                                           <  1:463491/149‑1 (MQ=255)                                                                                                                                                   gtatgttacAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGt                                                                                                                                                             >  1:305237/9‑155 (MQ=255)                                                                                                                                                   gtatgttacAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATgg                                                                                                                                                     <  1:707682/155‑1 (MQ=255)                                                                                                                                                    tatgttacAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAg                                                              <  1:737084/242‑1 (MQ=255)                                                                                                                                                       gttacAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTAtt                                                                                                                                                                            <  1:296677/132‑1 (MQ=255)                                                                                                                                                       gttacAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGtttat                                                                                                                                                         <  1:783842/151‑3 (MQ=255)                                                                                                                                                       gttacAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATgg                                                                                                                                         >  1:93089/5‑171 (MQ=255)                                                                                                                                                       gttacAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAgcg                                                                           >  1:848010/5‑233 (MQ=255)                                                                                                                                                       gttacAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGc                                                             >  1:707896/5‑247 (MQ=255)                                                                                                                                                       gttacAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGc                                                             >  1:85713/5‑247 (MQ=255)                                                                                                                                                            aaactgttcaacagTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATg                                                                     <  1:341961/226‑1 (MQ=255)                                                                                                                                                              actgttcaacagTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGaaaa                                                  <  1:740101/245‑1 (MQ=255)                                                                                                                                                               ctgttcaacagTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGCACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGaaaaa                                                 >  1:889162/6‑251 (MQ=255)                                                                                                                                                                 gttcaacagTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTAtt                                                                                                                                                                            <  1:113527/123‑1 (MQ=255)                                                                                                                                                                 gttcaacagTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATa                                               >  1:422696/4‑251 (MQ=255)                                                                                                                                                                 gttcaacagTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATa                                               >  1:808919/4‑251 (MQ=255)                                                                                                                                                                 gttcaacagTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATa                                               >  1:253734/4‑251 (MQ=255)                                                                                                                                                                      acagtGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAATGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAAc                                           >  1:492531/5‑250 (MQ=255)                                                                                                                                                                              cAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGAc                                  <  1:897788/251‑1 (MQ=255)                                                                                                                                                                                                            tatctcATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAAtc    <  1:350373/246‑2 (MQ=255)                                                                                                                                                                                                            tatctcATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAAtc    <  1:239945/246‑2 (MQ=255)                                                                                                                                                                                                              tctcATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAAAATTTGCGTCAAGGTGAAAtcac  <  1:142724/248‑4 (MQ=255)                                                                                                                                                                                                                 cATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGc                                                             >  1:393345/1‑189 (MQ=255)                                                                                                                                                                                                                            |                                                                                                                                                                                                                                             ATTTATAAACAACGTTGTGTATAAGGAGAACGTGATGAAAATGTTTAAAAAACTCACTTTAATTGCTGTTTTATCTATGCCGGTATTGGTACAAGCCAGCGGTATTCCGGTTGTTGATGCCGCAGCTGCCGGTCAACGTCAAATGAGCATGCTTCAAACGGTGCAACAATGGGCAAAAGAGGCTAAACAGTGGACGGATACAGTTTCGCATTATAAAAATGAGCTGAAAGCGTATGCCGACCAATTAGCCTCACAGACAGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTGTATGGTGAAATAAATGGCTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCTAAAAGCCTGTTTGAAAAATACAACCTTTTTGACCGTTGTAAGAATTTACGTCAAGGTGAAATTAC  >  1/1197980‑1198435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑