Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,198,175	G→A	intergenic (+38595/+222762)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,198,175	0	G	A	100.0%	162.5 / NA	62	intergenic (+38595/+222762)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/26);  total (36/26)

CCGCTAATGACTGGATTCCGATTTATAAACAACGTTGTGTATAAGGAGAACGTGATGAAAATGTTTAAAAAACTCACTTTAATTGCTGTTTTATCTATGCCGGTATTGGTACAAGCCAGCGGTATTCCGGTTGTTGATGCCGCAGCTGCCGGTCAACGTCAAATGAGCATGCTTCAAACGGTGCAACAATGGGCAAAAGAGGCTAAACAGTGGACGGATACAGTTTCGCATTATAAAAATGAGCTGAAAGCGTATGCCGACCAATTAGCCTCACAGACAGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTGTATGGTGAAATAAATGGCTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCTAAAAGCCTGTTTGAAAAATACAACCTTTTTGAC  >  1/1197960‑1198403                                                                                                                                                                                                                        |                                                                                                                                                                                                                                     ccGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACaaaaacgaactcaagg                                                                                                                                                                                                    >  1:308349/1‑239 (MQ=255) ccGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACaaaaacgaactcaagg                                                                                                                                                                                                    >  1:731531/1‑239 (MQ=255) ccGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACaaaaacgaactcaagg                                                                                                                                                                                                    >  1:86401/1‑239 (MQ=255)  cGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACaaaaacgaactcaaggcg                                                                                                                                                                                                  <  1:347358/251‑14 (MQ=255)                     aTTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGc                                                                                                                                                                                             <  1:237594/237‑1 (MQ=255)                      tttATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCt                                                                                                                                                                               >  1:567908/1‑250 (MQ=255)                                acGTTGTGTATAAGGAGAACGTGATGAAAATGTTTAAAAAACTCACTTTAATTGCTGTCTTGTCCATACCGGCATTAGTTCAAGCCAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGcc                                                                                                                                                                                >  1:679189/1‑239 (MQ=255)                                acGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGa                                                                                                                                                                    >  1:364165/1‑251 (MQ=255)                                  gTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAAt                                                                                                                                                                  >  1:799367/1‑251 (MQ=255)                                   ttGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAAt                                                                                                                                                                  <  1:342920/250‑1 (MQ=255)                                     gtgtATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCacag                                                                                                                                                                          <  1:336927/240‑1 (MQ=255)                                     gtgtATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCg                                                                                                                                                               >  1:58906/1‑251 (MQ=255)                                      tgtATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGt                                                                                                                                                              >  1:337627/1‑251 (MQ=255)                                       gtATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTg                                                                                                                                                             >  1:691574/1‑251 (MQ=255)                                       gtATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTg                                                                                                                                                             >  1:294045/1‑251 (MQ=255)                                       gtATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTg                                                                                                                                                             >  1:725014/1‑251 (MQ=255)                                           aaGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTg                                                                                                                                                             >  1:142079/1‑247 (MQ=255)                                                  aCGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAACACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGc                                                                                                                                                   >  1:592353/1‑250 (MQ=255)                                                          aaaaTCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTgg                                                                                                                                          <  1:828561/251‑1 (MQ=255)                                                                  tAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAgcgc                                                                                                                                  <  1:655244/251‑1 (MQ=255)                                                                            aCTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGtttata                                                                                                                        <  1:735545/251‑4 (MQ=255)                                                                             cTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTatat                                                                                                                       >  1:260477/1‑251 (MQ=255)                                                                             cTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTatat                                                                                                                       >  1:490461/1‑251 (MQ=255)                                                                                        gtcttgtCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCacagacc                                                                                                                                                                       <  1:872881/186‑2 (MQ=255)                                                                                        gtcttgtCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAAtt                                                                                                                                                                 <  1:177883/192‑1 (MQ=255)                                                                                            tgtCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATggt                                                                                                        >  1:25655/3‑250 (MQ=255)                                                                                                   accggcattagttcAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAAt                                                                                                 <  1:687568/238‑1 (MQ=255)                                                                                                   accggcattagttcAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAAt                                                                                                 <  1:244693/238‑1 (MQ=255)                                                                                                       gcattagttcAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGtttata                                                                                                                        >  1:809715/10‑221 (MQ=255)                                                                                                             gttcAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGtttata                                                                                                                        >  1:809530/4‑215 (MQ=255)                                                                                                               tcAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTAccc                                                                                     <  1:245680/250‑1 (MQ=255)                                                                                                                  agctaGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCacagacc                                                                                                                                                                       <  1:463962/162‑2 (MQ=255)                                                                                                                          gTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGAt                                                                          >  1:110855/1‑251 (MQ=255)                                                                                                                             ttCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTa                                                                       >  1:803527/1‑251 (MQ=255)                                                                                                                               ccGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACGAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGt                                                                     >  1:447852/1‑251 (MQ=255)                                                                                                                                  gtagttgatggtgctgCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAAt                                                                                                 <  1:620605/205‑1 (MQ=255)                                                                                                                                    agttgatggtgctgCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGaaat                                                                                                               <  1:695142/191‑1 (MQ=255)                                                                                                                                              gctgCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGGAGCGCATAGTTTATATg                                                                                                                      >  1:435093/4‑187 (MQ=255)                                                                                                                                                         tCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAgcg                                           <  1:703525/251‑1 (MQ=255)                                                                                                                                                                  aatcagtatgttacAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAg                                                                                                                                     >  1:114637/14‑152 (MQ=255)                                                                                                                                                                  aatcagtatgttacAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGcca                                  >  1:889212/14‑249 (MQ=255)                                                                                                                                                                    tcagtatgttacAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGaaataa                                                                                                             >  1:341936/12‑174 (MQ=255)                                                                                                                                                                       gtatgttacAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGcc                                                                                                                                                  <  1:66591/126‑1 (MQ=255)                                                                                                                                                                       gtatgttacAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGttt                                                                                                                           <  1:463491/149‑1 (MQ=255)                                                                                                                                                                       gtatgttacAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGt                                                                                                                             >  1:305237/9‑155 (MQ=255)                                                                                                                                                                       gtatgttacAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATgg                                                                                                                     <  1:707682/155‑1 (MQ=255)                                                                                                                                                                        tatgttacAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAg                              <  1:737084/242‑1 (MQ=255)                                                                                                                                                                           gttacAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTAtt                                                                                                                                            <  1:296677/132‑1 (MQ=255)                                                                                                                                                                           gttacAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGtttat                                                                                                                         <  1:783842/151‑3 (MQ=255)                                                                                                                                                                           gttacAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATgg                                                                                                         >  1:93089/5‑171 (MQ=255)                                                                                                                                                                           gttacAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAgcg                                           >  1:848010/5‑233 (MQ=255)                                                                                                                                                                           gttacAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGc                             >  1:707896/5‑247 (MQ=255)                                                                                                                                                                           gttacAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGc                             >  1:85713/5‑247 (MQ=255)                                                                                                                                                                                aaactgttcaacagTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATg                                     <  1:341961/226‑1 (MQ=255)                                                                                                                                                                                  actgttcaacagTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGaaaa                  <  1:740101/245‑1 (MQ=255)                                                                                                                                                                                   ctgttcaacagTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGCACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGaaaaa                 >  1:889162/6‑251 (MQ=255)                                                                                                                                                                                     gttcaacagTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTAtt                                                                                                                                            <  1:113527/123‑1 (MQ=255)                                                                                                                                                                                     gttcaacagTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATa               >  1:808919/4‑251 (MQ=255)                                                                                                                                                                                     gttcaacagTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATa               >  1:422696/4‑251 (MQ=255)                                                                                                                                                                                     gttcaacagTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATa               >  1:253734/4‑251 (MQ=255)                                                                                                                                                                                          acagtGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAATGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAAc           >  1:492531/5‑250 (MQ=255)                                                                                                                                                                                                  cAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCCAAAAGCCTGTTTGAAAAATACAACCTTTTTGAc  <  1:897788/251‑1 (MQ=255)                                                                                                                                                                                                                        |                                                                                                                                                                                                                                     CCGCTAATGACTGGATTCCGATTTATAAACAACGTTGTGTATAAGGAGAACGTGATGAAAATGTTTAAAAAACTCACTTTAATTGCTGTTTTATCTATGCCGGTATTGGTACAAGCCAGCGGTATTCCGGTTGTTGATGCCGCAGCTGCCGGTCAACGTCAAATGAGCATGCTTCAAACGGTGCAACAATGGGCAAAAGAGGCTAAACAGTGGACGGATACAGTTTCGCATTATAAAAATGAGCTGAAAGCGTATGCCGACCAATTAGCCTCACAGACAGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTGTATGGTGAAATAAATGGCTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCGCGAATGCTAAAAGCCTGTTTGAAAAATACAACCTTTTTGAC  >  1/1197960‑1198403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑