Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,198,124	G→C	intergenic (+38544/+222813)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,198,124	0	G	C	100.0%	143.1 / NA	50	intergenic (+38544/+222813)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (28/22);  total (28/22)

TCTGTCAACCAGTAAGGATAACGCAGAAATCCTACATCAAATTATCGAAGAAGTCGGCGAAGCCGCTAATGACTGGATTCCGATTTATAAACAACGTTGTGTATAAGGAGAACGTGATGAAAATGTTTAAAAAACTCACTTTAATTGCTGTTTTATCTATGCCGGTATTGGTACAAGCCAGCGGTATTCCGGTTGTTGATGCCGCAGCTGCCGGTCAACGTCAAATGAGCATGCTTCAAACGGTGCAACAATGGGCAAAAGAGGCTAAACAGTGGACGGATACAGTTTCGCATTATAAAAATGAGCTGAAAGCGTATGCCGACCAATTAGCCTCACAGACAGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTGTATGGTGAAATAAATGGCTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCG  >  1/1197898‑1198362                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                               tCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTcaacag                                                                                                                                                                                                                        <  1:135463/251‑2 (MQ=255) tCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTcaacag                                                                                                                                                                                                                        <  1:728130/251‑2 (MQ=255)         ccAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCaaa                                                                                                                                                                                                                >  1:598101/1‑251 (MQ=255)           aGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAag                                                                                                                                                                                                              >  1:644079/1‑251 (MQ=255)                ggATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCAGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTcaacag                                                                                                                                                                                                                        >  1:89648/1‑235 (MQ=255)                    aaCGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTTTACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAAc                                                                                                                                                                                                     <  1:737734/251‑1 (MQ=255)                              tcttACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGAcaga                                                                                                                                                                                           >  1:51409/4‑248 (MQ=255)                                    aTCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGAcagaaaccgt                                                                                                                                                                                     >  1:483430/1‑242 (MQ=255)                                         attattGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGAcagaaaccgtatctc                                                                                                                                                                                <  1:176362/251‑15 (MQ=255)                                                               ccGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACaaaaacgaactcaagg                                                                                                                                                           >  1:86401/1‑239 (MQ=255)                                                               ccGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACaaaaacgaactcaagg                                                                                                                                                           >  1:731531/1‑239 (MQ=255)                                                               ccGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACaaaaacgaactcaagg                                                                                                                                                           >  1:308349/1‑239 (MQ=255)                                                                cGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACaaaaacgaactcaaggcg                                                                                                                                                         <  1:347358/251‑14 (MQ=255)                                                                        attGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATgg                                                                                                                                                                                                <  1:329920/202‑1 (MQ=255)                                                                                   aTTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGc                                                                                                                                                    <  1:237594/237‑1 (MQ=255)                                                                                    tttATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCt                                                                                                                                      >  1:567908/1‑250 (MQ=255)                                                                                              acGTTGTGTATAAGGAGAACGTGATGAAAATGTTTAAAAAACTCACTTTAATTGCTGTCTTGTCCATACCGGCATTAGTTCAAGCCAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGcc                                                                                                                                       >  1:679189/1‑239 (MQ=255)                                                                                              acGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGa                                                                                                                           >  1:364165/1‑251 (MQ=255)                                                                                                gTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAAt                                                                                                                         >  1:799367/1‑251 (MQ=255)                                                                                                 ttGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAAt                                                                                                                         <  1:342920/250‑1 (MQ=255)                                                                                                   gtgtATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTcaacag                                                                                                                                                                                                                        <  1:227833/153‑2 (MQ=255)                                                                                                   gtgtATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCacag                                                                                                                                 <  1:336927/240‑1 (MQ=255)                                                                                                   gtgtATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCg                                                                                                                      >  1:58906/1‑251 (MQ=255)                                                                                                    tgtATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGt                                                                                                                     >  1:337627/1‑251 (MQ=255)                                                                                                     gtATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTg                                                                                                                    >  1:725014/1‑251 (MQ=255)                                                                                                     gtATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTg                                                                                                                    >  1:691574/1‑251 (MQ=255)                                                                                                     gtATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTg                                                                                                                    >  1:294045/1‑251 (MQ=255)                                                                                                         aaGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTg                                                                                                                    >  1:142079/1‑247 (MQ=255)                                                                                                                aCGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAACACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGc                                                                                                          >  1:592353/1‑250 (MQ=255)                                                                                                                        aaaaTCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTgg                                                                                                 <  1:828561/251‑1 (MQ=255)                                                                                                                                tAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAgcgc                                                                                         <  1:655244/251‑1 (MQ=255)                                                                                                                                          aCTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGtttata                                                                               <  1:735545/251‑4 (MQ=255)                                                                                                                                           cTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTatat                                                                              >  1:490461/1‑251 (MQ=255)                                                                                                                                           cTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTatat                                                                              >  1:260477/1‑251 (MQ=255)                                                                                                                                                      gtcttgtCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCacagacc                                                                                                                              <  1:872881/186‑2 (MQ=255)                                                                                                                                                      gtcttgtCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAAtt                                                                                                                        <  1:177883/192‑1 (MQ=255)                                                                                                                                                          tgtCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATggt                                                               >  1:25655/3‑250 (MQ=255)                                                                                                                                                                 accggcattagttcAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAAt                                                        <  1:687568/238‑1 (MQ=255)                                                                                                                                                                 accggcattagttcAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAAt                                                        <  1:244693/238‑1 (MQ=255)                                                                                                                                                                     gcattagttcAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGtttata                                                                               >  1:809715/10‑221 (MQ=255)                                                                                                                                                                           gttcAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGtttata                                                                               >  1:809530/4‑215 (MQ=255)                                                                                                                                                                             tcAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTAccc                                            <  1:245680/250‑1 (MQ=255)                                                                                                                                                                                agctaGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCacagacc                                                                                                                              <  1:463962/162‑2 (MQ=255)                                                                                                                                                                                        gTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGAt                                 >  1:110855/1‑251 (MQ=255)                                                                                                                                                                                           ttCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTa                              >  1:803527/1‑251 (MQ=255)                                                                                                                                                                                             ccGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACGAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGt                            >  1:447852/1‑251 (MQ=255)                                                                                                                                                                                                gtagttgatggtgctgCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAAt                                                        <  1:620605/205‑1 (MQ=255)                                                                                                                                                                                                  agttgatggtgctgCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGaaat                                                                      <  1:695142/191‑1 (MQ=255)                                                                                                                                                                                                            gctgCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGGAGCGCATAGTTTATATg                                                                             >  1:435093/4‑187 (MQ=255)                                                                                                                                                                                                                       tCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACAAAAACGAACTCAAGGCGTATGCCGACCAATTAGCCTCACAGACCGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTATATGGTGAAATAAATGGTTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAgcg  <  1:703525/251‑1 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                               TCTGTCAACCAGTAAGGATAACGCAGAAATCCTACATCAAATTATCGAAGAAGTCGGCGAAGCCGCTAATGACTGGATTCCGATTTATAAACAACGTTGTGTATAAGGAGAACGTGATGAAAATGTTTAAAAAACTCACTTTAATTGCTGTTTTATCTATGCCGGTATTGGTACAAGCCAGCGGTATTCCGGTTGTTGATGCCGCAGCTGCCGGTCAACGTCAAATGAGCATGCTTCAAACGGTGCAACAATGGGCAAAAGAGGCTAAACAGTGGACGGATACAGTTTCGCATTATAAAAATGAGCTGAAAGCGTATGCCGACCAATTAGCCTCACAGACAGGAATTCGTGATGTACAAGCCTTTATTGGTGAAGCGCATAGTTTGTATGGTGAAATAAATGGCTTAAAATCGGAGTTTACCCCGGTAATCGATTTAGTTTCCGGTGGGAAAAACGCATTAAGCG  >  1/1197898‑1198362

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑