Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,197,979	G→A	intergenic (+38399/+222958)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,197,979	0	G	A	100.0%	199.4 / NA	74	intergenic (+38399/+222958)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (37/37);  total (37/37)

CAAAGTAGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACCGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCCTACATCAAATTATCGAAGAAGTCGGCGAAGCCGCTAATGACTGGATTCCGATTTATAAACAACGTTGTGTATAAGGAGAACGTGATGAAAATGTTTAAAAAACTCACTTTAATTGCTGTTTTATCTATGCCGGTATTGGTACAAGCCAGCGGTATTCCGGTTGTTGATGCCGCAGCTGCCGGTCAACGTCAAATGAGCATGCTTCAAACGGTGCAACAATGGGCAAAAGAGGCTAAACAGTGGACGGATACAGTTTCGCATTATAAAAATGAGCTGAAAGCG  >  1/1197739‑1198211                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                         cAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAaac                                                                                                                                                                                                                                <  1:376801/251‑1 (MQ=255)       ggATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAaacaac                                                                                                                                                                                                                             >  1:86257/2‑248 (MQ=255)       ggATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGAATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGtt                                                                                                                                                                                                                          >  1:445174/2‑251 (MQ=255)            aTGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGta                                                                                                                                                                                                                     >  1:144466/1‑251 (MQ=255)             tgtgGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGtat                                                                                                                                                                                                                    <  1:649929/251‑1 (MQ=255)                  aTGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAgg                                                                                                                                                                                                                <  1:869660/250‑1 (MQ=255)                  aTGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAgg                                                                                                                                                                                                                <  1:280177/250‑1 (MQ=255)                  aTGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAgg                                                                                                                                                                                                                <  1:701495/250‑1 (MQ=255)                     gTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAaac                                                                                                                                                                                                                                <  1:459239/231‑1 (MQ=255)                     gTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAg                                                                                                                                                                                                                 <  1:18203/246‑1 (MQ=255)                     gTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGga                                                                                                                                                                                                               >  1:532746/1‑248 (MQ=255)                     gTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGaa                                                                                                                                                                                                            >  1:880424/1‑251 (MQ=255)                     gTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGATTAGGGCGTGAAAACTTAAAAATTCTGTAAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGga                                                                                                                                                                                                               >  1:284257/1‑248 (MQ=255)                     gTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGCGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGaa                                                                                                                                                                                                            >  1:416843/1‑251 (MQ=255)                              tCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGaa                                                                                                                                                                                                   <  1:624481/251‑1 (MQ=255)                                           aTTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAgg                                                                                                                                                                                                                >  1:393963/1‑225 (MQ=255)                                            tttGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAAc                                                                                                                                                                                     <  1:184391/251‑1 (MQ=255)                                                           gAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAg                                                                                                                                                                                                                 >  1:696031/1‑208 (MQ=255)                                                            aaTTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTg                                                                                                                                                                         >  1:451251/1‑247 (MQ=255)                                                              gtttcgagccctttacgcaCTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTg                                                                                                                                                                         >  1:750249/19‑245 (MQ=255)                                                                   aCCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGtcttgtct                                                                                                                                                              <  1:187684/251‑8 (MQ=255)                                                                         ttCCCGTACTTTTTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGTGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAgg                                                                                                                                                                                                                >  1:62856/1‑195 (MQ=255)                                                                           cccGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCgg                                                                                                                                                       >  1:564945/1‑250 (MQ=255)                                                                               tACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGc                                                                                                                                                                        <  1:35442/229‑1 (MQ=255)                                                                                   ttCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAaacaac                                                                                                                                                                                                                             >  1:466110/1‑172 (MQ=255)                                                                                          tGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGct                                                                                                                                         <  1:72966/249‑2 (MQ=255)                                                                                           gCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGctag                                                                                                                                       >  1:329409/1‑247 (MQ=255)                                                                                               aaaGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTa                                                                                                                                  <  1:897662/251‑1 (MQ=255)                                                                                                     cTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCgg                                                                                                                            <  1:56737/251‑1 (MQ=255)                                                                                                       cGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGgta                                                                                                                          >  1:842939/1‑250 (MQ=255)                                                                                                         aaaCAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGCAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAgg                                                                                                                                                                                                                >  1:430370/1‑163 (MQ=255)                                                                                                         aaaCAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGgtag                                                                                                                         <  1:149322/250‑3 (MQ=255)                                                                                                              gCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTAtac                                                                                                                                                          <  1:267966/212‑3 (MQ=255)                                                                                                              gCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATg                                                                                                                   >  1:372885/1‑251 (MQ=255)                                                                                                               ccTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGtagttg                                                                                                                      <  1:280069/247‑1 (MQ=255)                                                                                                                cTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATggt                                                                                                                 >  1:279456/1‑249 (MQ=255)                                                                                                                   aGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAgg                                                                                                                                                                                                                >  1:337497/1‑153 (MQ=255)                                                                                                                       aaGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATgg                                                                                                                  >  1:262707/1‑242 (MQ=255)                                                                                                                         gTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAgg                                                                                                                                                                                                                >  1:873780/1‑147 (MQ=255)                                                                                                                             gATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAgg                                                                                                                                                                                                                <  1:744903/143‑1 (MQ=255)                                                                                                                                       gTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAgg                                                                                                                                                                                                                >  1:119159/1‑133 (MQ=255)                                                                                                                                       gTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTAtac                                                                                                                                                          <  1:371586/187‑3 (MQ=255)                                                                                                                                       gTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATgg                                                                                                                  >  1:208441/1‑226 (MQ=255)                                                                                                                                       gTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAAt                                                                                          >  1:443052/1‑251 (MQ=255)                                                                                                                                       gTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAAt                                                                                          >  1:655629/1‑251 (MQ=255)                                                                                                                                              gCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAAtcagtat                                                                                    <  1:571004/250‑7 (MQ=255)                                                                                                                                                   aaaaCTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAAtcagtatgttac                                                                               <  1:773688/250‑12 (MQ=255)                                                                                                                                                      aCTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAAtcagtatgttacaaac                                                                           <  1:580475/251‑16 (MQ=255)                                                                                                                                                       cTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGc                                                                                                                                                                        >  1:691146/1‑157 (MQ=255)                                                                                                                                                              aTTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAAtcagtatgttacaaactgttcaac                                                                   <  1:34751/251‑24 (MQ=255)                                                                                                                                                              aTTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAAtcagtatgttacaaactgttcaac                                                                   <  1:20359/251‑24 (MQ=255)                                                                                                                                                                tCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTcaacag                                                                 <  1:728130/251‑2 (MQ=255)                                                                                                                                                                tCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTcaacag                                                                 <  1:135463/251‑2 (MQ=255)                                                                                                                                                                       aCCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAAtcagtatgttacaaactgttcaac                                                                   <  1:84945/242‑24 (MQ=255)                                                                                                                                                                        ccAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAAtcagtatgttacaaactgttcaac                                                                   >  1:133564/1‑218 (MQ=255)                                                                                                                                                                        ccAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCaaa                                                         >  1:598101/1‑251 (MQ=255)                                                                                                                                                                          aGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAag                                                       >  1:644079/1‑251 (MQ=255)                                                                                                                                                                               ggATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCgg                                                                                                                            <  1:874890/177‑1 (MQ=255)                                                                                                                                                                               ggATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCAGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTcaacag                                                                 >  1:89648/1‑235 (MQ=255)                                                                                                                                                                                   aaCGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTTTACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAAc                                              <  1:737734/251‑1 (MQ=255)                                                                                                                                                                                   aaCGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAAtcagtatgttacaaactgttcaac                                                                   <  1:336742/230‑24 (MQ=255)                                                                                                                                                                                    aCGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAAtc                                                                                         >  1:471331/1‑206 (MQ=255)                                                                                                                                                                                       cAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAAtcagtatgttacaaac                                                                           <  1:430051/218‑16 (MQ=255)                                                                                                                                                                                             tcttACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGAcaga                                    >  1:51409/4‑248 (MQ=255)                                                                                                                                                                                                   aTCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGAcagaaaccgt                              >  1:483430/1‑242 (MQ=255)                                                                                                                                                                                                        attattGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGAcagaaaccgtatctc                         <  1:176362/251‑15 (MQ=255)                                                                                                                                                                                                           attgaagaaGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATggtgctgctgc                                                                                                         <  1:172880/165‑11 (MQ=255)                                                                                                                                                                                                                       ggCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTGGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAAtcagtatgttacaaac                                                                           <  1:689314/186‑16 (MQ=255)                                                                                                                                                                                                                              ccGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACaaaaacgaactcaagg    >  1:731531/1‑239 (MQ=255)                                                                                                                                                                                                                              ccGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACaaaaacgaactcaagg    >  1:86401/1‑239 (MQ=255)                                                                                                                                                                                                                              ccGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACaaaaacgaactcaagg    >  1:308349/1‑239 (MQ=255)                                                                                                                                                                                                                               cGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATGGACAGAAACCGTATCTCATTACaaaaacgaactcaaggcg  <  1:347358/251‑14 (MQ=255)                                                                                                                                                                                                                                 cTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAAtcagtatgttacaaac                                                                           <  1:267871/176‑16 (MQ=255)                                                                                                                                                                                                                                       attGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGGTAGTTGATGGTGCTGCTGCAGGTCAACGGCAAATCAGTATGTTACAAACTGTTCAACAGTGGGCAAAAGAAGCAAAACAATgg                                         <  1:329920/202‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                         CAAAGTAGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACCGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCCTACATCAAATTATCGAAGAAGTCGGCGAAGCCGCTAATGACTGGATTCCGATTTATAAACAACGTTGTGTATAAGGAGAACGTGATGAAAATGTTTAAAAAACTCACTTTAATTGCTGTTTTATCTATGCCGGTATTGGTACAAGCCAGCGGTATTCCGGTTGTTGATGCCGCAGCTGCCGGTCAACGTCAAATGAGCATGCTTCAAACGGTGCAACAATGGGCAAAAGAGGCTAAACAGTGGACGGATACAGTTTCGCATTATAAAAATGAGCTGAAAGCG  >  1/1197739‑1198211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑