Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,197,847	C→A	intergenic (+38267/+223090)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,197,847	0	C	A	100.0%	206.6 / NA	76	intergenic (+38267/+223090)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (45/31);  total (45/31)

GAGAAACAACCAAATACCGATGACCCAAAGTATGGATGAAGTGTTGAAAAGCCCAATTTCTCGCGCGGTAGTTGAGGAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCACGCAAAGTAGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACCGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCCTACATCAAATTATCGAAGAAGTCGGCGAAGCCGCTAATGACTGGATTCCGATTTATAAACAACGTTGTGTATAAGGAGAACGTGATGAAAATGTTTAAAAAACTCACTTTAATTGCTGTTTTATCTATGCCGGTATTGGTACAAGCCAGCGGTATTCCGGTTG  >  1/1197622‑1198092                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      agatggaattgttaTACCGATGACCCAAAGTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGc                                                                                                                                                                                                                                               >  1:291606/14‑234 (MQ=255)         ttgttaTACCGATGACCCAAAGTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAggg                                                                                                                                                                                                                      >  1:551424/6‑251 (MQ=255)         ttgttaTACCGATGACCCAAAGTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAggg                                                                                                                                                                                                                      <  1:526553/246‑1 (MQ=255)         ttgttaTACCGATGACCCAAAGTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAggg                                                                                                                                                                                                                      >  1:586823/6‑251 (MQ=255)         ttgttaTACCGATGACCCAAAGTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAggg                                                                                                                                                                                                                      <  1:617088/246‑1 (MQ=255)         ttgttaTACCGATGACCCAAAGTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAggg                                                                                                                                                                                                                      >  1:743301/6‑251 (MQ=255)         ttgttaTACCGATGACCCAAAGTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAggg                                                                                                                                                                                                                      >  1:753838/6‑251 (MQ=255)         ttgttaTACCGATGACCCAAAGTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAggg                                                                                                                                                                                                                      <  1:145040/246‑1 (MQ=255)            ttaTACCGATGACCCAAAGTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGt                                                                                                                                                                                                                   >  1:61885/3‑251 (MQ=255)                              gTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCt                                                                                                                                                                                                                                              >  1:110964/1‑206 (MQ=255)                              gTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAggg                                                                                                                                                                                                                      >  1:584936/1‑230 (MQ=255)                              gTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAAtt                                                                                                                                                                                                    >  1:534334/1‑248 (MQ=255)                                aTGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTA‑GTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCa                                                                                                                                                                                              >  1:195667/1‑251 (MQ=255)                                         gtgtTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAggg                                                                                                                                                                                                                      >  1:178129/1‑219 (MQ=255)                                         gtgtTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTaa                                                                                                                                                                                      >  1:739408/1‑251 (MQ=255)                                         gtgtTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTaa                                                                                                                                                                                      >  1:854387/1‑251 (MQ=255)                                         gtgtTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTaa                                                                                                                                                                                      >  1:851997/1‑251 (MQ=255)                                         gtgtTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTaa                                                                                                                                                                                      >  1:750601/1‑251 (MQ=255)                                         gtgtTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTaa                                                                                                                                                                                      >  1:149394/1‑251 (MQ=255)                                          tgtTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTaa                                                                                                                                                                                      <  1:599479/250‑1 (MQ=255)                                            ttGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAgg                                                                                                                                                                                    <  1:456069/250‑1 (MQ=255)                                                   gCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCa                                                                                                                                                                            >  1:791288/1‑251 (MQ=255)                                                             ccgcgcgGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTAc                                                                                                                                                                  <  1:322879/250‑1 (MQ=255)                                                                cgcgGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATc                                                                                                                                                               <  1:672786/251‑1 (MQ=255)                                                                    gTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAAt                                                                                                                                                           >  1:807295/1‑251 (MQ=255)                                                                     tAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGc                                                                                                                                                                             >  1:181386/1‑232 (MQ=255)                                                                      aGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGAt                                                                                                                                                                                  >  1:488331/1‑226 (MQ=255)                                                                       gTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTAt                                                                                                                                                        >  1:871563/1‑251 (MQ=255)                                                                               aTGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAAtt                                                                                                                                                          <  1:633343/241‑1 (MQ=255)                                                                               aTGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGaagaag                                                                                                                                                >  1:164158/1‑251 (MQ=255)                                                                               aTGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGaagaag                                                                                                                                                <  1:659899/251‑1 (MQ=255)                                                                                        aacCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAg                                                                                                                                       >  1:632697/1‑251 (MQ=255)                                                                                         acCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGAAAACGcttcatcttttaacc                                                                                                                                                               <  1:257141/226‑15 (MQ=255)                                                                                               tGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTaa                                                                                                                                >  1:178652/1‑251 (MQ=255)                                                                                                    aCCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGatt                                                                                                                           >  1:553512/1‑249 (MQ=255)                                                                                                       tAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGa                                                                                                                        >  1:730905/1‑251 (MQ=255)                                                                                                         aCCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGAtt                                                                                                                      >  1:839225/1‑251 (MQ=255)                                                                                                              aTTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTccaat                                                                                                                 <  1:128730/251‑4 (MQ=255)                                                                                                                  cccGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTgaag                                                                                                                                                   >  1:61151/3‑213 (MQ=255)                                                                                                                   gcGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGAt                                                                                                                                                                                  <  1:512314/180‑1 (MQ=255)                                                                                                                      cAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAaac                                                                                                         <  1:376801/251‑1 (MQ=255)                                                                                                                       aaaGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGcc                                                                                                                                                                                                                                                    <  1:872009/111‑1 (MQ=255)                                                                                                                            ggATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGc                                                                                                                                                                                                                     <  1:255172/136‑1 (MQ=255)                                                                                                                            ggATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAaacaac                                                                                                      >  1:86257/2‑248 (MQ=255)                                                                                                                            ggATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGAATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGtt                                                                                                   >  1:445174/2‑251 (MQ=255)                                                                                                                                 aTGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGta                                                                                              >  1:144466/1‑251 (MQ=255)                                                                                                                                  tgtgGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGtat                                                                                             <  1:649929/251‑1 (MQ=255)                                                                                                                                       aTGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAgg                                                                                         <  1:869660/250‑1 (MQ=255)                                                                                                                                       aTGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAgg                                                                                         <  1:701495/250‑1 (MQ=255)                                                                                                                                       aTGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAgg                                                                                         <  1:280177/250‑1 (MQ=255)                                                                                                                                          gTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAaac                                                                                                         <  1:459239/231‑1 (MQ=255)                                                                                                                                          gTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAg                                                                                          <  1:18203/246‑1 (MQ=255)                                                                                                                                          gTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGga                                                                                        >  1:532746/1‑248 (MQ=255)                                                                                                                                          gTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGaa                                                                                     >  1:880424/1‑251 (MQ=255)                                                                                                                                          gTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGATTAGGGCGTGAAAACTTAAAAATTCTGTAAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGga                                                                                        >  1:284257/1‑248 (MQ=255)                                                                                                                                          gTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGCGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGaa                                                                                     >  1:416843/1‑251 (MQ=255)                                                                                                                                            ttCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGa                                                                                                                                         <  1:130807/197‑1 (MQ=255)                                                                                                                                                   tCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGaa                                                                            <  1:624481/251‑1 (MQ=255)                                                                                                                                                                aTTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAgg                                                                                         >  1:393963/1‑225 (MQ=255)                                                                                                                                                                 tttGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAAc                                                              <  1:184391/251‑1 (MQ=255)                                                                                                                                                                                gAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAg                                                                                          >  1:696031/1‑208 (MQ=255)                                                                                                                                                                                 aaTTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTg                                                  >  1:451251/1‑247 (MQ=255)                                                                                                                                                                                   gtttcgagccctttacgcaCTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTg                                                  >  1:750249/19‑245 (MQ=255)                                                                                                                                                                                        aCCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGtcttgtct                                       <  1:187684/251‑8 (MQ=255)                                                                                                                                                                                              ttCCCGTACTTTTTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGTGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAgg                                                                                         >  1:62856/1‑195 (MQ=255)                                                                                                                                                                                                cccGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCgg                                >  1:564945/1‑250 (MQ=255)                                                                                                                                                                                                  cagaCTTTCTTAGTGCGTAAAGGGCACGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAAAACTGTCAACCAGTAAGGATAACGCAGAAATCATACATCAAATTAAAGAAGAAGTTGGCGAAGCCGCTAATg                                                                                                                              <  1:708177/151‑1 (MQ=255)                                                                                                                                                                                                    tACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGc                                                 <  1:35442/229‑1 (MQ=255)                                                                                                                                                                                                        ttCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAaacaac                                                                                                      >  1:466110/1‑172 (MQ=255)                                                                                                                                                                                                               tGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGct                  <  1:72966/249‑2 (MQ=255)                                                                                                                                                                                                                gCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGctag                >  1:329409/1‑247 (MQ=255)                                                                                                                                                                                                                    aaaGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTa           <  1:897662/251‑1 (MQ=255)                                                                                                                                                                                                                          cTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCgg     <  1:56737/251‑1 (MQ=255)                                                                                                                                                                                                                            cGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGgta   >  1:842939/1‑250 (MQ=255)                                                                                                                                                                                                                              aaaCAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGCAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAgg                                                                                         >  1:430370/1‑163 (MQ=255)                                                                                                                                                                                                                              aaaCAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAAACAACGTTGTGTATAAGGAGAACGTAATGAAAATCTTTAAAAAACTCACTTTAATTGCTGTCTTGTCTATACCGGCATTAGTTCAAGCTAGCGGTATTCCGgtag  <  1:149322/250‑3 (MQ=255)                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                      GAGAAACAACCAAATACCGATGACCCAAAGTATGGATGAAGTGTTGAAAAGCCCAATTTCTCGCGCGGTAGTTGAGGAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCACGCAAAGTAGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACCGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCCTACATCAAATTATCGAAGAAGTCGGCGAAGCCGCTAATGACTGGATTCCGATTTATAAACAACGTTGTGTATAAGGAGAACGTGATGAAAATGTTTAAAAAACTCACTTTAATTGCTGTTTTATCTATGCCGGTATTGGTACAAGCCAGCGGTATTCCGGTTG  >  1/1197622‑1198092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑