Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,197,745	A→G	intergenic (+38165/+223192)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,197,745	0	A	G	100.0%	170.7 / NA	60	intergenic (+38165/+223192)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/24);  total (36/24)

GAGAAACAACCAAATACCGATGACCCAAAGTATGGATGAAGTGTTGAAAAGCCCAATTTCTCGCGCGGTAGTTGAGGAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCACGCAAAGTAGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACCGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCCTACATCAAATTATCGAAGAAGTCGGCGAAGCCGCTAATGACTGGATTCCGATTTATAAAC  >  1/1197622‑1197989                                                                                                                            |                                                                                                                                                                                                                                                     agatggaattgttaTACCGATGACCCAAAGTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGc                                                                                                                                        >  1:291606/14‑234 (MQ=255)         ttgttaTACCGATGACCCAAAGTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATgg                                                                                                                                                                                                                                        <  1:431875/125‑1 (MQ=255)         ttgttaTACCGATGACCCAAAGTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAgg                                                                                                                                                          <  1:419967/203‑1 (MQ=255)         ttgttaTACCGATGACCCAAAGTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAg                                                                                                                                                           <  1:757303/202‑1 (MQ=255)         ttgttaTACCGATGACCCAAAGTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAAc                                                                                                                                                 <  1:119024/212‑1 (MQ=255)         ttgttaTACCGATGACCCAAAGTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAggg                                                                                                               >  1:753838/6‑251 (MQ=255)         ttgttaTACCGATGACCCAAAGTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAggg                                                                                                               <  1:617088/246‑1 (MQ=255)         ttgttaTACCGATGACCCAAAGTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAggg                                                                                                               >  1:743301/6‑251 (MQ=255)         ttgttaTACCGATGACCCAAAGTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAggg                                                                                                               <  1:526553/246‑1 (MQ=255)         ttgttaTACCGATGACCCAAAGTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAggg                                                                                                               >  1:586823/6‑251 (MQ=255)         ttgttaTACCGATGACCCAAAGTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAggg                                                                                                               >  1:551424/6‑251 (MQ=255)         ttgttaTACCGATGACCCAAAGTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAggg                                                                                                               <  1:145040/246‑1 (MQ=255)           gttaTACCGATGACCCAAAGTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGaa                                                                                                                                                                                                                                <  1:187834/133‑1 (MQ=255)            ttaTACCGATGACCCAAAGTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGt                                                                                                            >  1:61885/3‑251 (MQ=255)                  cGATGACCCAAAGTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTAc                                                                                                                                                                            >  1:634774/1‑181 (MQ=255)                      gACCCAAAGTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATg                                                                                                                                                                                                                                         <  1:32390/116‑1 (MQ=255)                      gACCCAAAGTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAgg                                                                                                                                                          >  1:210560/1‑195 (MQ=255)                            aaGTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGaa                                                                                                                                                                                                                                <  1:323269/119‑1 (MQ=255)                              gTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGaa                                                                                                                                                                                                                                >  1:351878/1‑117 (MQ=255)                              gTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAAc                                                                                                                                                                                                                    >  1:516453/1‑129 (MQ=255)                              gTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTcc                                                                                                                                                                                 >  1:627749/1‑164 (MQ=255)                              gTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCt                                                                                                                                       >  1:110964/1‑206 (MQ=255)                              gTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAggg                                                                                                               >  1:584936/1‑230 (MQ=255)                              gTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAAtt                                                                                             >  1:534334/1‑248 (MQ=255)                                aTGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTA‑GTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCa                                                                                       >  1:195667/1‑251 (MQ=255)                                         gtgtTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAAcc                                                                                                                                                                                        >  1:56101/1‑146 (MQ=255)                                         gtgtTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAAc                                                                                                                                                                                         <  1:729603/145‑1 (MQ=255)                                         gtgtTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAggg                                                                                                               >  1:178129/1‑219 (MQ=255)                                         gtgtTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTaa                                                                               >  1:739408/1‑251 (MQ=255)                                         gtgtTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTaa                                                                               >  1:750601/1‑251 (MQ=255)                                         gtgtTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTaa                                                                               >  1:149394/1‑251 (MQ=255)                                         gtgtTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTaa                                                                               >  1:854387/1‑251 (MQ=255)                                         gtgtTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTaa                                                                               >  1:851997/1‑251 (MQ=255)                                          tgtTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTaa                                                                               <  1:599479/250‑1 (MQ=255)                                            ttGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAgg                                                                             <  1:456069/250‑1 (MQ=255)                                                   gCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCa                                                                     >  1:791288/1‑251 (MQ=255)                                                    cccAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAAt                                                                                                                                                                                                                               <  1:184333/96‑1 (MQ=255)                                                             ccgcgcgGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTAc                                                           <  1:322879/250‑1 (MQ=255)                                                                cgcgGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATc                                                        <  1:672786/251‑1 (MQ=255)                                                                    gTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAAt                                                    >  1:807295/1‑251 (MQ=255)                                                                     tAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGc                                                                      >  1:181386/1‑232 (MQ=255)                                                                      aGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGAt                                                                           >  1:488331/1‑226 (MQ=255)                                                                       gTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTAc                                                                                                                                                                            >  1:885855/1‑128 (MQ=255)                                                                       gTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTAt                                                 >  1:871563/1‑251 (MQ=255)                                                                               aTGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAAtt                                                   <  1:633343/241‑1 (MQ=255)                                                                               aTGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGaagaag                                         >  1:164158/1‑251 (MQ=255)                                                                               aTGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGaagaag                                         <  1:659899/251‑1 (MQ=255)                                                                                        aacCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAg                                >  1:632697/1‑251 (MQ=255)                                                                                         acCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGAAAACGcttcatcttttaacc                                                        <  1:257141/226‑15 (MQ=255)                                                                                           cATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAg                                                                                                                                                                    >  1:811347/1‑116 (MQ=255)                                                                                               tGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTaa                         >  1:178652/1‑251 (MQ=255)                                                                                                    aCCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAAcagc                                                                                                                                              <  1:358533/129‑4 (MQ=255)                                                                                                    aCCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGatt                    >  1:553512/1‑249 (MQ=255)                                                                                                       tAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGa                 >  1:730905/1‑251 (MQ=255)                                                                                                         aCCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGAtt               >  1:839225/1‑251 (MQ=255)                                                                                                              aTTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTccaat          <  1:128730/251‑4 (MQ=255)                                                                                                                  cccGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTgaag                                            >  1:61151/3‑213 (MQ=255)                                                                                                                   gcGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGAt                                                                           <  1:512314/180‑1 (MQ=255)                                                                                                                      cAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCTTACATCAAATTATTGAAGAAGTTGGCGAAGCCGCTAATGATTGGATTCCAATTTATAaac  <  1:376801/251‑1 (MQ=255)                                                                                                                       aaaGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGcc                                                                                                                                             <  1:872009/111‑1 (MQ=255)                                                                                                                            |                                                                                                                                                                                                                                                     GAGAAACAACCAAATACCGATGACCCAAAGTATGGATGAAGTGTTGAAAAGCCCAATTTCTCGCGCGGTAGTTGAGGAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCACGCAAAGTAGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACCGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCAGAAATCCTACATCAAATTATCGAAGAAGTCGGCGAAGCCGCTAATGACTGGATTCCGATTTATAAAC  >  1/1197622‑1197989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑