Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,197,682	T→C	intergenic (+38102/+223255)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,197,682	0	T	C	100.0%	97.8 / NA	37	intergenic (+38102/+223255)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/14);  total (23/14)

GAGAAACAACCAAATACCGATGACCCAAAGTATGGATGAAGTGTTGAAAAGCCCAATTTCTCGCGCGGTAGTTGAGGAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCACGCAAAGTAGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACCGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCA  >  1/1197622‑1197922                                                             |                                                                                                                                                                                                                                                 agatggaattgttaTACCGATGACCCAAAGTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGc                                                                     >  1:291606/14‑234 (MQ=255)         ttgttaTACCGATGACCCAAAGTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATgg                                                                                                                                                                     <  1:431875/125‑1 (MQ=255)         ttgttaTACCGATGACCCAAAGTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAgg                                                                                       <  1:419967/203‑1 (MQ=255)         ttgttaTACCGATGACCCAAAGTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAg                                                                                        <  1:757303/202‑1 (MQ=255)         ttgttaTACCGATGACCCAAAGTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAAc                                                                              <  1:119024/212‑1 (MQ=255)         ttgttaTACCGATGACCCAAAGTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAggg                                            <  1:617088/246‑1 (MQ=255)         ttgttaTACCGATGACCCAAAGTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAggg                                            >  1:586823/6‑251 (MQ=255)         ttgttaTACCGATGACCCAAAGTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAggg                                            >  1:551424/6‑251 (MQ=255)         ttgttaTACCGATGACCCAAAGTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAggg                                            <  1:526553/246‑1 (MQ=255)         ttgttaTACCGATGACCCAAAGTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAggg                                            >  1:743301/6‑251 (MQ=255)         ttgttaTACCGATGACCCAAAGTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAggg                                            >  1:753838/6‑251 (MQ=255)         ttgttaTACCGATGACCCAAAGTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAggg                                            <  1:145040/246‑1 (MQ=255)           gttaTACCGATGACCCAAAGTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGaa                                                                                                                                                             <  1:187834/133‑1 (MQ=255)            ttaTACCGATGACCCAAAGTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGt                                         >  1:61885/3‑251 (MQ=255)                  cGATGACCCAAAGTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTAc                                                                                                         >  1:634774/1‑181 (MQ=255)                      gACCCAAAGTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATg                                                                                                                                                                      <  1:32390/116‑1 (MQ=255)                      gACCCAAAGTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAgg                                                                                       >  1:210560/1‑195 (MQ=255)                            aaGTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGaa                                                                                                                                                             <  1:323269/119‑1 (MQ=255)                              gTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGaa                                                                                                                                                             >  1:351878/1‑117 (MQ=255)                              gTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAAc                                                                                                                                                 >  1:516453/1‑129 (MQ=255)                              gTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTcc                                                                                                              >  1:627749/1‑164 (MQ=255)                              gTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCt                                                                    >  1:110964/1‑206 (MQ=255)                              gTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAggg                                            >  1:584936/1‑230 (MQ=255)                              gTATGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAAtt                          >  1:534334/1‑248 (MQ=255)                                aTGGATGAAGTGTTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTA‑GTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCa                    >  1:195667/1‑251 (MQ=255)                                         gtgtTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAAcc                                                                                                                     >  1:56101/1‑146 (MQ=255)                                         gtgtTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAAc                                                                                                                      <  1:729603/145‑1 (MQ=255)                                         gtgtTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAggg                                            >  1:178129/1‑219 (MQ=255)                                         gtgtTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTaa            >  1:739408/1‑251 (MQ=255)                                         gtgtTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTaa            >  1:750601/1‑251 (MQ=255)                                         gtgtTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTaa            >  1:149394/1‑251 (MQ=255)                                         gtgtTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTaa            >  1:851997/1‑251 (MQ=255)                                         gtgtTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTaa            >  1:854387/1‑251 (MQ=255)                                          tgtTGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTaa            <  1:599479/250‑1 (MQ=255)                                            ttGAAAAGCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAgg          <  1:456069/250‑1 (MQ=255)                                                   gCCCAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACAGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCa  >  1:791288/1‑251 (MQ=255)                                                    cccAATTTCCCGCGCGGTAGTTGAGCAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCCCGCAAAGTGGATTATGTGGATGGTTTCGGAAt                                                                                                                                                            <  1:184333/96‑1 (MQ=255)                                                             |                                                                                                                                                                                                                                                 GAGAAACAACCAAATACCGATGACCCAAAGTATGGATGAAGTGTTGAAAAGCCCAATTTCTCGCGCGGTAGTTGAGGAATGTGAAACAACCATTTGTTTACCTAACCCGATTGCACGCAAAGTAGATTATGTGGATGGTTTCGGAATCAGCGAAAAACAATTTGAAATTATCCGTGAATTACAACCGGATTCCCGTACTTTCTTAGTGCGTAAAGGGCTCGAAACCGCCTTAGCTAAGTTAGATTTAAGCGGTTTAGGGCGTGAAAACTTAAAAATTCTGTCAACCAGTAAGGATAACGCA  >  1/1197622‑1197922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑