Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,191,463	G→A	intergenic (+31883/+229474)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,191,463	0	G	A	100.0%	157.1 / NA	55	intergenic (+31883/+229474)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (39/16);  total (39/16)

TGCGTGGCCTTATGCGTAATGCGTCTATTCTGATTCTGGACGAACCCACCGCCTCTTTAACGCCAATTGAAACGGAGAACCTGTTTAATAAAATTCGTTCGTTATTGGAAAAGGGTGTGGGCATTGTCTTCATTTCTCACAAACTGCCCGAAGTGTGGCAGATTTCTCATCGTATTAGCGTCATGCGCGACGGCTATATTGCACTAACCGGCAGTACTGCCGAGTTGAATAAAGACGATGTGATTACCGCCATCACGCCGAAGGCGAAAAATGCCCAATTAACGGAAAGCCAAAAATTATGGCTGGCGTTGCCGGGACATAACCGTGTGAAAGCTACCGATAAGCCGATTTTGCAGGTGCAGGATTTGACGGGCGAAGGATTTAAAAATGTCACTTTTGATATTTATCCCGGTGAAATTTTAGGCTTGGCGGGCGTGGTTGGCGCAGGGCGAACCGAAT  >  1/1191240‑1191698                                                                                                                                                                                                                                |                                                                                                                                                                                                                                            tGCGTGGCCTTATGCGTAATGCGTCTATTCTGATTCTGGACGACCCCACCGCCTCTTTAACGCCAATTGAAACGGAGAACCTGTTTAATAAAATTCGTTCGTTATTGGAAAAGGGTGTGGGCATTGTCTTCATTTCTCACAAACTGCCCGAAGTGTGGCAGATTTCTCATCGTATTAGCGTCATGCGCGACGGCTATATTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGACGATGTGATTACCGc                                                                                                                                                                                                                   <  1:401854/250‑1 (MQ=255)       gCCTTATGCGTAATGCGTCTATTCTGATTCTGGACGAACCCACCGCCTCTTTAACGCCAATTGAAACGGAGAACCTGTTTAATAAAATTCGTTCGTTATTGGAAAAGGGTGTGGGCATTGTCTTCATTTCTCACAAACTGCCCGAAGTGTGGCAGATTTCTCATCGTATTAGCGTCATGCGCGACGGCTATATTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGa                                                                                                                                                                                                                                  >  1:614404/1‑229 (MQ=255)       gCCTTATGCGTAATGCGTCTATTCTGATTCTGGACGAACCCACCGCCTCTTTAACGCCAATTGAAACGGAGAACCTGTTTAATAAAATTCGTTCGTTATTGGAAAAGGGTGTGGGCATTGTCTTCATTTCTCACAAACTGCCCGAAGTGTGGCAGATTTCTCATCGTATTAGCGTCATGCGCGACGGCTATATTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGACGATGTGATTACCGCCa                                                                                                                                                                                                                 >  1:369729/1‑246 (MQ=255)         cTTATGCGTAATGCGTCTATTCTGATTCTGGACGAACCCACCGCCTCTTTAACGCCAATTGAAACGGAGAACCTGTTTAATAAAATTCGTTCGTTATTGGAAAAGGGTGTGGGCATTGTCTTCATTTCTCACAAACTGCCCGAAGTGTGGCAGATTTCTCATCGTATTAGCGTCATGCGCGACGGCTATATTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGACGATGTGATTACCGCCATCACGc                                                                                                                                                                                                           >  1:164924/1‑250 (MQ=255)             tGCGTAATGCGTCTATTCTGATTCTGGACGAACCCACCGCCTCTTTAACGCCAATTGAAACGGAGAACCTGTTTAATAAAATTCGTTCGTTATTGGAAAAGGGTGTGGGCATTGTCTTCATTTCTCACAAACTGCCCGAAGTGTGGCAGATTTCTCATCGTATTAGCGTCATGCGCGACGGCTATATTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGAc                                                                                                                                                                                                                                 >  1:377829/1‑224 (MQ=255)                      cGTCTATTCTGATTCTGGACGAACCCACCGCCTCTTTAACGCCAATTGAAACGGAGAACCTGTTTAATAAAATTCGTTCGTTATTGGAAAAGGGTGTGGGCATTGTCTTCATTTCTCACAAACTGCCCGAAGTGTGGCAGATTTCTCATCGTATTAGCGTCATGCGCGACGGCTATATTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGACGATGTGAt                                                                                                                                                                                                                         >  1:295785/1‑223 (MQ=255)                          tATTCTGATTCTGGACGAACCCACCGCCTCTTTAACGCCAATTGAAACGGAGAACCTGTTTAATAAAATTCGTTCGTTATTGGAAAAGGGTGTGGGCATTGTCTTCATTTCTCACAAACTGCCCGAAGTGTGGCAGATTTCTCATCGTATTAGCGTCATGCGCGACGGCTATATTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGACGATGTGATTACCGCCATCACGCCGAAGGCGAAAAATGcc                                                                                                                                                                                          >  1:793703/1‑250 (MQ=255)                            ttctgattctgGACGAACCCACCGCCTCTTTAACGCCAATTGAAACGGAGAACCTGTTTAATAAAATTCGTTCGTTATTGGAAAAGGGTGTGGGCATTGTCTTCATTTCTCACAAACTGCCCGAAGTGTGGCAGATTTCTCATCGTATTAGCGTCATGCGCGACGGCTATATTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGa                                                                                                                                                                                                                                  >  1:527813/1‑208 (MQ=255)                            ttctgattctgGACGAACCCACCGCCTCTTTAACGCCAATTGAAACGGAGAACCTGTTTAATAAAATTCGTTCGTTATTGGAAAAGGGTGTGGGCATTGTCTTCATTTCTCACAAACTGCCCGAAGTGTGGCAGATTTCTCATCGTATTAGCGTCATGCGCGACGGCTATATTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGACGATGTGATTACCGCCATCACGCCGAAGGCGAAAAATGCCCaa                                                                                                                                                                                       >  1:544965/1‑251 (MQ=255)                            ttctgattctgGACGAACCCACCGCCTCTTTAACGCCAATTGAAACGGAGAACCTGTTTAATAAAATTCGTTCGTTATTGGAAAAGGGTGTGGGCATTGTCTTCATTTCTCACAAACTGCCCGAAGTGTGGCAGATTTCTCATCGTATTAGCGTCATGCGCGACGGCTATATTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGACGATGTGATTACCGCCATCACGCCGAAGGCGAAAAATGCCCaa                                                                                                                                                                                       >  1:689012/1‑251 (MQ=255)                                gattctgGACGAACCCACCGCCTCTTTAACGCCAATTGAAACGGAGAACCTGTTTAATAAAATTCGTTCGTTATTGGAAAAGGGTGTGGGCATTGTCTTCATTTCTCACAAACTGCCCGAAGTGTGGCAGATTTCTCATCGTATTAGCGTCATGCGCGACGGCTATATTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGACGATGTGATTACCGcc                                                                                                                                                                                                                  <  1:290288/220‑1 (MQ=255)                                               cACCGCCTCTTTAACGCCAATTGAAACGGAGAACCTGTTTAATAAAATTCGTTCGTTATTGGAAAAGGGTGTGGGCATTGTCTTCATTTCTCACAAACTGCCCGAAGTGTGGCAGATTTCTCATCGTATTAGCGTCATGCGCGACGGCTATATTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGACGATGTGATTACCGCCATCACGCCGAAGGCGAAAAATGCCCAATTAACGGAAAGCCAAAAAt                                                                                                                                                                    <  1:235069/251‑1 (MQ=255)                                                 ccGCCTCTTTAACGCCAATTGAAACGGAGAACCTGTTTAATAAAATTCGTTCGTTATTGGAAAAGGGTGTGGGCATTGTCTTCATTTCTCACAAACTGCCCGAAGTGTGGCAGATTTCTCATCGTATTAGCGTCATGCGCGACGGCTATATTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGACGATGTGATTACCGCCATCACGCCGAAGGCGAAAAATGCCCAATTAACGGAAAGCCAAAAATTa                                                                                                                                                                  >  1:370564/1‑251 (MQ=255)                                                                 aaTTGAAACGGAGAACCTGTTTAATAAAATTCGTTCGTTATTGGAAAAGGGTGTGGGCATTGTCTTCATTTCTCACAAACTGCCCGAAGTGTGGCAGATTTCTCATCGTATTAGCGTCATGCGCGACGGCTATATTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGACGATGTGATTACCGCCATCACGCCGAAGGCGAAAAATGCCCAATTAACGGAAAGCCAAAAATTATGGCTGGCGTTGCCgg                                                                                                                                                  >  1:284651/1‑251 (MQ=255)                                                                 aaTTGAAACGGAGAACCTGTTTAATAAAATTCGTTCGTTATTGGAAAAGGGTGTGGGCATTGTCTTCATTTCTCACAAACTGCCAGAAGTGTGGCAGATTTCTCATCGTATTAGCGTCATGCGCGACGGCTATATTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGACGATGTGATTAcc                                                                                                                                                                                                                     >  1:314729/1‑184 (MQ=255)                                                                         cGGAGAACCTGTTTAATAAAATTCTTTCGTTATTGGAAAAGGGTGTGGGCATTGTCTTCATTTCTCACAAACTTCCCGAAGTGTGGCAGATTTCTCATCGTATTAGCGTCATGCGCGACGGCTATATTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGACGATGTGATTACCGCCATCACGCCGAAGGCGAAAAATGCCCAATTAACGGAAAGCCAAAAATTATGGCTGGCGTTGCCGGGACATAAc                                                                                                                                          <  1:338321/251‑1 (MQ=255)                                                                         cGGAGAACCTGTTTAATAAAATTCGTTCGTTATTGGAAAAGGGTGTGGGCATTGTCTTCATTTCTCACAAACTGCCCGAAGTGTGGCAGATTTCTCATCGTATTAGCGTCATGCGCGACGGCTATATTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGACGATGTGATTACCGCCATCACGCCGAAGGCGAAAAATGCCCAATTAACGGAAAGCCAAAAATTATGGCTGGCGTTGCCGGGACATAAc                                                                                                                                          <  1:200351/251‑1 (MQ=255)                                                                         cGGAGAACCTGTTTAATAAAATTCGTTCGTTATTGGAAAAGGGTGTGGGCATTGTCTTCATTTCTCACAAACTGCCCGAAGTGTGGCAGATTTCTCATCGTATTAGCGTCATGCGCGACGGCTATATTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGACGATGTGATTACCGCCATCACGCCGAAGGCGAAAAATGCCCAATTAACGGAAAGCCAAAAATTATGGCTGGCGTTGCCGGGACATAAc                                                                                                                                          <  1:365481/251‑1 (MQ=255)                                                                           gagaACCTGTTTAATAAAATTCGTTCGTTATTGGAAAAGGGTGTGGGCATTGTCTTCATTTCTCACAAACTGCCCGAAGTGTGGCAGATTTCTCATCGTATTAGCGTCATGCGCGACGGCTATATTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGACGATGTGATTACCGCCATCACGCCGAAGGCGAAAAATGCCCAATTAACGGAAAGCCAAAAATTATGGCTGGCGTTGCCGGGACATAACCg                                                                                                                                        >  1:801930/1‑251 (MQ=255)                                                                             gaACCTGTTTAATAAAATTCGTTCGTTATTGGAAAAGGGTGTGGGCATTGTCTTCATTTCTCACAAACTGCCCGAAGTGTGGCAGATTTCTCATCGTATTAGCGTCATGCGCGACGGCTATATTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGACGATGTGATTACCGCCATCACGCCGAAGGCGAAAAATGCCCAATTAACGGAAAGCCAAAAATTATGGCTGGCGTTGCCGGGACATAACCgtg                                                                                                                                      >  1:74658/1‑251 (MQ=255)                                                                                   gTTTAATAAAATTCGTTCGTTATTGGAAAAGGGTGTGGGCATTGTCTTCATTTCTCACAAACTGCCCGAAGTGTGGCAGATTTCTCATCGTATTAGCGTCATGCGCGACGGCTATATTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGACGATGTGATTACCGCCATCACGCCGAAGGCGAAAAATGCCCAATTAACGGAAAGCCAAAAATTATGGCTGGCGTTGCCGGGACATAAc                                                                                                                                          <  1:852943/241‑1 (MQ=255)                                                                                                      ttATTGGAAAAGGGTGTGGGCATTGTCTTCATTTCTCACAAACTGCCCGAAGTGTGGCAGATTTCTCATCGTATTAGCGTCATGCGCGACGGCTATATTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGACGATGTGATTACCGCCATCACGCCGAAGGCGAAAAATGCCCAATTAACGGAAAGCCAAAAATTATGGCTGGCGTTGCCGGGACATAAc                                                                                                                                          <  1:173384/222‑1 (MQ=255)                                                                                                         ttGGAAAAGGGTG‑GGGCATTGTCTTCATTTCTCACAAACTGCCCGAAGTGTGGCAGATTTCTCATCGTATTAGCGTCATGCGCGACGGCTATATTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGACGATGTGATTACCGCCATCACGCCGAAGGCGAAAAATGCCCAATTAACGGAAAGCCAAAAATTATGGCTGGCGTTGCCGGGACATAACCGTGTGAAAGCTACCGATAAGCCGATTTtgcag                                                                                                         >  1:856936/1‑251 (MQ=255)                                                                                                           ggAAAAGGGTGTGGGCATTGTCTTCATTTCTCACAAACTGCCCGAAGTGTGGCAGATTTCTCATCGTATTAGCGTCATGCGCGACGGCTATATTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGACGATGTGATTACCGc                                                                                                                                                                                                                   >  1:293410/1‑144 (MQ=255)                                                                                                                  ggTGTGGGCATTGTCTTCATTTCTCACAAACTGCCCGAAGTGTGGCAGATTTCTCATCGTATTAGCGTCATGCGCGACGGCTATATTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGACGATGTGATTACCGCCATCACGCCGAAGGCGAAAAATGCCCAATTAACGGAAAGCCAAAAATTATGGCTGGCGTTGCCGGGAc                                                                                                                                               >  1:279078/1‑205 (MQ=255)                                                                                                                  ggTGTGGGCATTGTCTTCATTTCTCACAAACTGCCCGAAGTGTGGCAGATTTCTCATCGTATTAGCGTCATGCGCGACGGCTATATTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGACGATGTGATTACCGCCATCACGCCGAAGGCGAAAAATGCCCAATTAACGGAAAGCCAAAAATTATGGCTGGCGTTGCCGGGACATAACCGTGTGAAAGCTACCGATAAGCCGATTTTGCAGGTGCAGGa                                                                                                 >  1:473668/1‑251 (MQ=255)                                                                                                                    tgtgGGCATTGTCTTCATTTCTCACAAACTGCCCGAAGTGTGGCAGATTTCTCATCGTATTAGCGTCATGCGCGACGGCTATATTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGACGATGTGATTACCGCCATCACGCCGAAGGCGAAAAATGCCCAATTAACGGAAAGCCAAAAATTATGGCTGGCGTTGCCGGGACATAACCGTGTGAAAGCTACCGATAAGCCGATTTTGCAGGTGCAGGAt                                                                                                <  1:764617/250‑1 (MQ=255)                                                                                                                              gTCTTCATTTCTCACAAACTGCCCGAAGTGTGGCAGATTTCTCATCGTATTAGCGTCATGCGCGACGGCTATATTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGACGATGTGATTACCGCCATCACGCCGAAGGCGAAAAATGCCCAATTAACGGAAAGCCAAAAATTATGGCTGGCGTTGCCGGGACa                                                                                                                                              <  1:154/194‑1 (MQ=255)                                                                                                                              gTCTTCATTTCTCACAAACTGCCCGAAGTGTGGCAGATTTCTCATCGTATTAGCGTCATGCGCGACGGCTATATTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGACGATGTGATTACCGCCATCACGCCGAAGGCGAAAAATGCCCAATTAACGGAAAGCCAAAAATTATGGCTGGCGTTGCCGGGACATAACCGTGTGAAAGCTACCGATAAGCCGATTTtgca                                                                                                          >  1:96041/1‑230 (MQ=255)                                                                                                                              gTCTTCATTTCTCACAAACTGCCCGAAGTGTGGCAGATTTCTCATCGTATTAGCGTCATGCGCGACGGCTATATTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGACGATGTGATTACCGCCATCACGCCGAAGGCGAAAAATGCCCAATTAACGGAAAGCCAAAAATTATGGCTGGCGTTGCCGGGACATAACCGTGTGAAAGCTACCGATAAGCCGATTTtg                                                                                                            >  1:184935/1‑228 (MQ=255)                                                                                                                                        ctcACAAACTGCCCGAAGTGTGGCAGATTTCTCATCGTATTAGCGTCATGCGCGACGGCTATATTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGACGATGTGATTACCGCCATCACGCCGAAGGCGAAAAATGCCCAATTAACGGAAAGCCAAAAATTAt                                                                                                                                                                 >  1:862646/1‑165 (MQ=255)                                                                                                                                                cTGCCCGAAGTGTGGCAGATTTCTCATCGTATTAGCGTCATGCGCGACGGCTATATTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGACGATGTGATTACCGCCATCACGCCGAAGGCGAAAAATGCCCAATTAACGGAAAGCCAAAAATTATGGCTGGCGTTGCCGGGACATAACCGTGTGAAAGCTACCGATAAGCCGATTTTGCAGGTGCAGGATTTGACGGGCGAAGGATTTAAAAATGTCa                                                                    >  1:51260/1‑250 (MQ=255)                                                                                                                                                  gCCCGAAGTGTGGCAGATTTCTCATCGTATTAGCGTCATGCGCGACGGCTATATTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGACGATGTGATTACCGCCATCACGCCGAAGGCGAAAAATGCCCAATTAACGGAAAGCCAAAAATTATGGCTGGCGTTGCCGGGACATAACCGTGTGAAAGCTACCGATAAGCCGATTTTGCAGGTGCAGGATTTGACGGGCGAAGGATTTAAAAATGTCACtt                                                                 >  1:827493/1‑251 (MQ=255)                                                                                                                                                         gtgtGGCAGATTTCTCATCGTATTAGCGTCATGCGCGACGGCTATATTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGACGATGTGATTACCGCCATCACGCCGAAGGCGAAAAATGCCCAATTAACGGAAAGCCAAAAATTATGGCTGGCGTTGCCGGGACATAAc                                                                                                                                          >  1:699472/1‑171 (MQ=255)                                                                                                                                                         gtgtGGCAGATTTCTCATCGTATTAGCGTCATGCGCGACGGCTATATTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGACGATGTGATTACCGCCATCACGCCGAAGGCGAAAAATGCCCAATTAACGGAAAGCCAAAAATTATGGCTGGCGTTGCCGGGACATAAc                                                                                                                                          >  1:825990/1‑171 (MQ=255)                                                                                                                                                          tgtgGCAGATTTCTCATCGTATTAGCGTCATGCGCGACGGCTATATTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGACGATGTGATTACCGCCATCACGCCGAAGGCGAAAAATGCCCAATTAACGGAAAGCCAAAAATTATGGCTGGCGTTGCCGGGACATAACCGTGTGAAAGCTACCGATAAGCCGATTTTGCAGGTGCAGGATTTGACGGGCGAAGGATTTAAAAATGTCACTTTTGata                                                           <  1:16840/249‑1 (MQ=255)                                                                                                                                                          tgtgGCAGATTTCTCATCGTATTAGCGTCATGCGCGACGGCTATATTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGACGATGTGATTACCGCCATCACGCCGAAGGCGAAAAATGCCCAATTAACGGAAAGCCAAAAATTATGGCTGGCGTTGCCGGGACATAACCGTGTGAAAGCTACCGATAAGCCGATTTTGCAGGTGCAGGATTTGACGGGCGAAGGATTTAAAAATGTCACTTTTGata                                                           <  1:678054/249‑1 (MQ=255)                                                                                                                                                           gtgGCAGATTTCTCATCGTATTAGCGTCATGCGCGACGGCTATATTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGACGATGTGATTACCGCCATCACGCCGAAGGCGAAAAATGCCCAATTAACGGAAAGCCAAAAATTATGGCTGGCGTTGCCGGGACATAACCGTGTGAAAGCTACCGATAAGCCGATTTTGCAGGTGCAGGAt                                                                                                >  1:730779/1‑211 (MQ=255)                                                                                                                                                                            gTATTAGCGTCATGCGCGACGGCTATATTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGACGATGTGATTACCGCCATCACGCCGAAGGCGAAAAATGCCCAATTAACGGAAAGCCAAAAATTATGGCTGGCGTTGCCGGGACATAACCGTGTGAAAGCTACCGATAAGCCGATTTTGCAGGTGCAGGATTTGACGGGCGAAGGATTTAAAAATGTCACTTTTGATATTTATCCCGGTGAAATTTTa                                       >  1:42827/1‑251 (MQ=255)                                                                                                                                                                            gTATTAGCGTCATGCGCGACGGCTATATTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGACGATGTGATTACCGCCATCACGCCGAAGGCGAAAAATGCCCAATTAACGGAAAGCCAAAAATTATGGCTGGCGTTGCCGGGACATAACCGTGTGAAAGCTACCGATAAGCCGATTTTGCAGGTGCAGGATTTGACGGGCGAAGGATTTAAAAATGTCACTTTTGATATTTATCCCGGTGAAATTTTa                                       >  1:307204/1‑251 (MQ=255)                                                                                                                                                                            gTATTAGCGTCATGCGCGACGGCTATATTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGACGATGTGATTACCGCCATCACGCCGAAGGCGAAAAATGCCCAATTAACGGAAAGCCAAAAATTATGGCTGGCGTTGCCGGGACATAACCGTGTGAAAGCTACCGATAAGCCGATTTTGCAGGTGCAGGATTTGACGGGCGAAGGATTTAAAAATGTCACTTTTGATATTTATCCCGGTGAAATTTTa                                       >  1:515428/1‑251 (MQ=255)                                                                                                                                                                             tATTAGCGTCATGCGCGACGGCTATATTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGACGATGTGATTACCGCCATCACGCCGAAGGCGAAAAATGCCCAATTAACGGAAAGCCAAAAATTATGGCTGGCGTTGCCGGGACATAACCGTGTGAAAGCTACCGATAAGCCGATTTTGCAGGTGCAGGATTTGACGGGCGAAGGATTTAAAAATGTCACTTTTGATATTTATCCCGGTGAAATTTTAg                                      >  1:859956/1‑251 (MQ=255)                                                                                                                                                                             tATTAGCGTCATGCGCGACGGCTATATTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGACGATGTGATTACCGCCATCACGCCGAAGGCGAAAAATGCCCAATTAACGGAAAGCCAAAAATTATGGCTGGCGTTGCCGGGACATAACCGTGTGAAAGCTACCGATAAGCCGATTTTGCAGGTGCAGGATTTGACGGGCGAAGGATTTAAAAATGTCACTTTTGATATTTATCCCGGTGAAATTTTAg                                      >  1:257603/1‑251 (MQ=255)                                                                                                                                                                              aTTAGCGTCATGCGCGACGGCTATATTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGACGATGTGATTACCGCCATCACGCCGAAGGCGAAAAATGCCCAATTAACGGAAAGCCAAAAATTATGGCTGGCGTTGCCGGGACATAACCGTGTGAAAGCTACCGATAAGCCGATTTTGCAGGTGCAGGATTTGACGGGCGAAGGATTTAAAAATGTCACTTTTGATATTTATCCCGGTGAAATTTTAgg                                     <  1:339650/251‑1 (MQ=255)                                                                                                                                                                                               cGGCTATATTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGACGATGTGATTACCGCCATCACGCCGAAGGCGAAAAATGCCCAATTAACGGAAAGCCAAAAATTATGGCTGGCGTTGCCGGGACATAACCg                                                                                                                                        <  1:50384/135‑1 (MQ=255)                                                                                                                                                                                               cGGCTATATTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGACGATGTGATTACCGCCATCACGCCGAAGGCGAAAAATGCCCAATTAACGGAAAGCCAAAAATTATGGCTGGCGTTGCCGGGACATAACCGTGTGAAAGCTACCGATAAGCCGATTTTGCAGGTGCAGGATTTGACGGGCGAAGGATTTAAAAATGTCACTTTTGATATTTATCCCGGTGAAATTTTAGGCTTGGCGGGCGTGGTTg                    >  1:318424/1‑251 (MQ=255)                                                                                                                                                                                                 gCTATATTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGACGATGTGATTACCGCCATCACGCCGAAGGCGAAAAATGCCCAATTAACGGAAAGCCAAAAATTATGGCTGGCGTTGCCGGGACATAACCGTGTGAAAGCTACCGATAAGCCGATTTTGCAGGTGCAGGATTTGACGGGCGAAGGATTTAAAAATGTCACTTTTGATATTTATCCCGGTGAAATTTTAGGCTTGGCGGGCGTGGTTggg                  >  1:27871/1‑250 (MQ=255)                                                                                                                                                                                                 gCTATATTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGACGATGTGATTACCGCCATCACGCCGAAGGCGAAAAATGCCCAATTAACGGAAAGCCAAAAATTATGGCTGGCGTTGCCGGGACATAACCGTGTGAAAGCTACCGATAAGCCGATTTTGCAGGTGCAGGATTTGACGGGCGAAGGATTTAAAAATGTCACTTTTGATATTTATCCCGGTGAAATTTTAGGCTTGGCGGGCGTGGTTgg                   >  1:806485/1‑250 (MQ=255)                                                                                                                                                                                                    atatTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGACGATGTGATTACCGCCATCACGCCGAAGGCGAAAAATGCCCAATTAACGGAAAGCCAAAAATTATGGCTGGCGTTGCCGGGACATAACCGTGTGAAAGCTACCGATAAGCCGATTTTGCAGGTGCAGGATTTGACGGGCGAAGGATTTAAAAATGTCACTTTTGATATTTATCCCGGTGAAATTTTAGGCTTGGCGGGCGTGGTTGgcgc                <  1:758233/250‑1 (MQ=255)                                                                                                                                                                                                    atatTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGACGATGTGATTACCGCCATCACGCCGAAGGCGAAAAATGCCCAATTAACGGAAAGCCAAAAATTATGGCTGGCGTTGCCGGGACATAACCGTGTGAAAGCTACCGATAAGCCGATTTTGCAGGTGCAGGATTTGACGGGCGAAGGATTTAAAAATGTCACTTTTGATATTTATCCCGGTGAAATTTTAGGCTTGGCGGGCGTGGTTGGCGCa               >  1:398628/1‑251 (MQ=255)                                                                                                                                                                                                    atatTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGACGATGTGATTACCGCCATCACGCCGAAGGCGAAAAATGCCCAATTAACGGAAAGCCAAAAATTATGGCTGGCGTTGCCGGGACATAACCGTGTGAAAGCTACCGATAAGCCGATTTTGCAGGTGCAGGATTTGACGGGCGAAGGATTTAAAAATGTCACTTTTGATATTTATCCCGGTGAAATTTTAGGCTTGGCGGGCGTGGGTGGCGCa               >  1:692668/1‑251 (MQ=255)                                                                                                                                                                                                    atatTGCACTAACCGGCAGTACTGCCGAATTGAATAAAGACGATGTGATTACCGCCATCACGCCGAAGGCGAAAAATGCCCAATTAACGGAAAGCCAAAAATTATGGCTGGCGTTGCCGGGACATAACCGTGTGAAAGCTACCGATAAGCCGATTTTGCAGGTGCAGGATTTGACGGGCGAAGGATTTAAAAATGTCACTTTTGATATTTATCCCGGCGAAATTTTAGGCTTGGCGGGCGTGGTTGGCGCa               >  1:30451/1‑251 (MQ=255)                                                                                                                                                                                                        tGCACTAACCGGCAGTACTGCCGAATTGAATAAAGACGATGTGATTACCGCCATCACGCCGAAGGCGAAAAATGCCCAATTAACGGAAAGCCAAAAATTATGGCTGGCGTTGCCGGGACATAACCGTGTGAAAGCTACCGATAAGCCGATTTTGCAGGTGCAGGATTTGACGGGCGAAGGATTTAAAAATGTCACTTTTGATATTTATCCCGGTGAAATTTTAGGCTTGGCGGGCGTGGTTGGCGCAGGGc           <  1:296601/251‑1 (MQ=255)                                                                                                                                                                                                              aaCCGGTAGTACTGCCGAATTGAATAAAGACGATGTGATTACCGCCATCACGCCGAAGGCGAAAAATGCCCAATTAACGGAAAGCCAAAAATTATGGCTGGCGTTGCCGGGACATAACCGTGTGAAAGCTACCGATAAGCCGATTTTGCAGGTGCAGGATCTGACGGGCGAAGGATTTAAAAATGTCACTTTTGATATTTATCCCGGTGAAATTTTAGGCTTGGCGGGCGTGGTTGGCGCAGGGCGAACCg     >  1:557283/1‑251 (MQ=255)                                                                                                                                                                                                                 cGGCAGTACTGCCGAATTGAATAAAGACGATGTGATTACCGCCATCACGCCGAAGGCGAAAAATGCCCAATTAACGGAAAGCCAAAAATTATGGCTGGCGTTGCCGGGACATAACCGTGTGAAAGCTACCGATAAGCCGATTTTGCAGGTGCAGGATTTGACGGGCGAAGGATTTAAAAATGTCACTTTTGATATTTATCCCGGTGAAATTTTAGGCTTGGCGGGCGTGGTTGGCGCAGGGCGAAACGAAt  >  1:351483/1‑251 (MQ=255)                                                                                                                                                                                                                                |                                                                                                                                                                                                                                            TGCGTGGCCTTATGCGTAATGCGTCTATTCTGATTCTGGACGAACCCACCGCCTCTTTAACGCCAATTGAAACGGAGAACCTGTTTAATAAAATTCGTTCGTTATTGGAAAAGGGTGTGGGCATTGTCTTCATTTCTCACAAACTGCCCGAAGTGTGGCAGATTTCTCATCGTATTAGCGTCATGCGCGACGGCTATATTGCACTAACCGGCAGTACTGCCGAGTTGAATAAAGACGATGTGATTACCGCCATCACGCCGAAGGCGAAAAATGCCCAATTAACGGAAAGCCAAAAATTATGGCTGGCGTTGCCGGGACATAACCGTGTGAAAGCTACCGATAAGCCGATTTTGCAGGTGCAGGATTTGACGGGCGAAGGATTTAAAAATGTCACTTTTGATATTTATCCCGGTGAAATTTTAGGCTTGGCGGGCGTGGTTGGCGCAGGGCGAACCGAAT  >  1/1191240‑1191698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑