Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,190,829	C→T	intergenic (+31249/+230108)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,190,829	0	C	T	100.0%	132.1 / NA	47	intergenic (+31249/+230108)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/18);  total (29/18)

TATCAATTGTTCTTACCGTTTTTTTGTAAAAGCATGATGGAGATCACTTTTTTAAAAATTAAAGGCGATTTTTAGATAATCTTATCCAGATTTGTGATCTAGTTCTCAGAAATTTTGTGCATTCCTGACTAGTATTTTGAAAAAATGTTTTTTTTAAATACATTTGTTCATTCTATGCGGCAGGTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAATATCCGCCCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTCGGCGGTAACGGGGCGGGGAAATCCACGCTGATGAAAATTATCGCCGGCATTCAGCCGCAAGATAGCGGAGAACTGGAGGTTCGGGGTGCGACAAAATAAGCTTTCTCCGAATAAAGCCCATCAGCTAGGTATTTATTTGGTGCCGCAGGAACCG  >  1/1190598‑1191070                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                  tatCAATTGTTCTTACCGTTTTTTTGTAAAAGCATGATGGAGATCACTTTTTTAAAAATTAAAGGCGATTTTTAGATAATCTTATCCAGATTTGTGATCTAGTTCTCAGAAATTTTGTGCATTCCTGACTAGTATTTTAAAAAAATGTTTTTTTTAAATACATTTGTTCATTCTATGCGGCAGGTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGt                                                                                                                                                                                                                                >  1:840746/1‑251 (MQ=255)                                                         aaTTAAAGGCGATTTTTAGATAATCTTATCCAGATTTGTGATCTAGTTCTCAGAAATTTTGTGCATTCCTGACTAGTATTTTAAAAAAATGTTTTTTTTAAATACATTTGTTCATTCTATGCGGCAGGTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTg                                                                                                                                                                        >  1:328985/1‑251 (MQ=255)                                                                 gCGATTTTTAGATAATCTTATCCAGATTTGTGATCTAGTTCTCAGAAATTTTGTGCATTCCTGACTAGTATTTTAAAAAAATGTTTTTTTTAAATACATTTGTTCATTCTATGCGGCAGGTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTgcacgcac                                                                                                                                                                 <  1:837841/250‑1 (MQ=255)                                                                     tttttAGATAATCTTATCCAGATTTGTGATCTAGTTCTCAGAAATTTTGTGCATTCCTGACTAGTATTTTAAAAAAATGTTTTTTTTAAATACATTTGTTCATTCTATGCGGCAGGTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGc                                                                                                                                                                                  <  1:391742/229‑1 (MQ=255)                                                                               aTCTTATCCAGATTTGTGATCTAGTTCTCAGAAATTTTGTGCATTCCTGACTAGTATTTTAAAAAAATGTTTTTTTTAAATACATTTGTTCATTCTATGCGGCAGGTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGcc                                                                                                                                                                                                      <  1:208877/199‑1 (MQ=255)                                                                               aTCTTATCCAGATTTGTGATCTAGTTCTCAGAAATTTTGTGCATTCCTGACTAGTATTTTAAAAAAATGTTTTTTTTAAATACATTTGTTCATTCTATGCGGCAGGTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTcggcgg                                                                                                                                                       >  1:348258/1‑246 (MQ=255)                                                                                    aTCCAGATTTGTGATCTAGTTCTCAGAAATTTTGTGCATTCCTGACTAGTATTTTAAAAAAATGTTTTTTTTAAATACATTTGTTCATTCTATGCGGCAGGTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTCGGCGGTAAcggggcg                                                                                                                                             >  1:486868/1‑251 (MQ=255)                                                                                         gATTTGTGATCTAGTTCTCAGAAATTTTGTGCATTCCTGACTAGTATTTTAAAAAAATGTTTTTTTTAAATACATTTGTTCATTCTATGCGGCAGGTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTCGGCGGTAACGGGGCGGGGaa                                                                                                                                        >  1:770588/1‑251 (MQ=255)                                                                                            ttGTGATCTAGTTCTCAGAAATTTTGTGCATTCCTGACTAGTATTTTAAAAAAATGTTTTTTTTAAATACATTTGTTCATTCTATGCGGCAGGTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTCGGCGGTAACGGGGCGGGGAAAt                                                                                                                                      >  1:659379/1‑250 (MQ=255)                                                                                                                          ttCCTGACTAGTATTTTAAAAAAATGTTTTTTTTAAATACATTTGTTCATTCTATGCGGCAGGTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTCGGCGGTAACGGGGCGGGGAAATCCACGCTGATGAAAATTATCGCCGGCATTc                                                                                                        <  1:880307/250‑1 (MQ=255)                                                                                                                          ttCCTGACTAGTATTTTAAAAAAATGTTTTTTTTAAATACATTTGTTCATTCTATGCGGCAGGTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTCGGCGGTAACGGGGCGGGGAAATCCACGCTGATGAAAATTATCGCCGGCATTCa                                                                                                       >  1:741126/1‑251 (MQ=255)                                                                                                                            ccTGACTAGTATTTTAAAAAAATGTTTTTTTTAAATACATTTGTTCATTCTATGCGGCAGGTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTgcacgca                                                                                                                                                                  <  1:151273/190‑1 (MQ=255)                                                                                                                               gACTAGTATTTTAAAAAAATGTTTTTTTTAAATACATTTGTTCATTCTATGCGGCAGGTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTCGGCGGTAAcgggg                                                                                                                                               >  1:822660/1‑206 (MQ=255)                                                                                                                               gACTAGTATTTTAAAAAAATGTTTTTTTTAAATACATTTGTTCATTCTATGCGGCAGGTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTCGGCGGTAACGGGGCGGGGAAATCCACGCTGATGAAAAtt                                                                                                                     >  1:312919/1‑232 (MQ=255)                                                                                                                                                 aTGTTTTTTTTAAATACATTTGTTCATTCTATGCGGCAGGTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTCGGCGGTAACGGGGCGGGGAAATCCACGCTGATGAAAATTATCGCCGGCATTCAGCCGCAAGATAGCGGAGAACTgg                                                                                <  1:29688/251‑1 (MQ=255)                                                                                                                                                      ttttttAAATACATTTGTTCATTCTATGCGGCAGGTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTCGGCGGTAACGGGGCGGGGAAATCCACGCTGATGAAAATTATCGCCGGCATTCAGCCGCAAGATAGCGGAGAACTGGAGGtt                                                                           <  1:241813/251‑1 (MQ=255)                                                                                                                                                            aaaTACATTTGTTCATTCTATGCGGCAGGTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTCGGCGGTAACGGGGCGGGGAAATCCAAGCTGATGAAAATTATCGCCGGCATTCAGCCGCAAGATAGCGCAGAACTGGAGGTTCggggg                                                                     >  1:314817/1‑250 (MQ=255)                                                                                                                                                                aCATTTGTTCATTCTATGCGGCAGGTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTCGGCGGTAACGGGGCGGGGAAATCCACGCTGATGAAAATTATCGCCGGCATTCAGCCGCAAGAt                                                                                             <  1:541616/223‑1 (MQ=255)                                                                                                                                                                 cATTTGTTCATTCTATGCGGCAGGTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTCGGCGGTAACGGGGCGGGGAAATCCACGCTGATGAAAATTATCGCCGGCATTCAGCCGCAAGATAGCGGAGAACTGGAGGTTCGGGGTGCGa                                                                 >  1:32228/1‑250 (MQ=255)                                                                                                                                                                 cATTTGTTCATTCTATGCGGCAGGTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTCGGCGGTAACGGGGCGGGGAAATCCACGCTGATGAAAATTATCGCCGGCATTCAGCCGCAAGATAGCGGAGAACTGGAGGTTCGGGGTGCGAc                                                                <  1:284326/251‑1 (MQ=255)                                                                                                                                                                     tGTTCATTCTATGCGGCAGGTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTg                                                                                                                                                                        >  1:460162/1‑143 (MQ=255)                                                                                                                                                                     tGTTCATTCTATGCGGCAGGTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTCGGCGGTAACGGGGCGGGGAAATCCACGCTGATGAAAATTATCGCCGGCATTCAGCCGCAAGATAGCGGAGAACTGGAg                                                                              >  1:573243/1‑233 (MQ=255)                                                                                                                                                                     tGTTCATTCTATGCGGCAGGTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTCGGCGGTAACGGGGCGGGGAAATCCACGCTGATGAAAATTATCGCCGGCATTCAGCCGCAAGATAGCGGAGAACTGGAGGtt                                                                           >  1:899345/1‑236 (MQ=255)                                                                                                                                                                     tGTTCATTCTATGCGGCAGGTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTCGGCGGTAACGGGGCGGGGAAATCCACGCTGATGAAAATTATCGCCGGCATTCAGCCGCAAGATAGCGGAGAACTGGAGGTTCGGGGTGCGACaaaa                                                            >  1:837598/1‑251 (MQ=255)                                                                                                                                                                           ttCTATGCGGCAGGTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTCGGCGGTAACGGGGCGGGGAAATCCACGCTGATGAAAATTATCGCCGGCATTCAGCCGCAAGATAGCGGAGAACTGGAGGTTCGGGGTGCGACAAAATAAGCt                                                      <  1:770849/251‑1 (MQ=255)                                                                                                                                                                             cTATGCGGCAGGTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTCGGCGGTAACGGGGCGGGGAAATCCACGCTGATGAAAATTATCGCCGGCATTCAGCCGCAAGATAGCGGAGAACTGGAGGTTCGGGGCGCGACAAAATAAGCttt                                                    >  1:203286/1‑251 (MQ=255)                                                                                                                                                                              tATGCGGCAGGTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTCGGCGGTAACGGGGCGGGGAAATCCACGCTGATGAAAATTATCGCCGGCATTCAGCCGCAAGATAGCGGAGAACTGGAGGTTCGGGGTGCGACAAAATAAGCTTtc                                                   <  1:759501/251‑1 (MQ=255)                                                                                                                                                                                    gCAGGTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTCGGCGGTAACGGGGCGGGGAAATCCACGCTGATGAAAATTATCGCCGGCATTCAGCCGCAAGATAGCGGAGAACTGGAGGTTCGGGGTGCGACCAAATAAGCTTTCTCCGaa                                             >  1:489764/1‑251 (MQ=255)                                                                                                                                                                                       ggTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTCGGCGGTaa                                                                                                                                                    <  1:574372/145‑1 (MQ=255)                                                                                                                                                                                       ggTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTCGGCGGTAACGGGGCGGGGAAATCCACGCTGATGAAAATTATCGCCGGCATTCAGCCGCAAGATAGCGGAGAACTGGAGGTTCGGGGTGCGACAAAATAAGCTTTCTCCGAAt                                            <  1:338517/249‑1 (MQ=255)                                                                                                                                                                                               aTGCAAACTCACATTTCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTCGGCGGTAACGGGGCGGGGAAATCCACGCTGATGAAAATTATCGCCGGCATTCAGCCGCAAGATAGCGGAGAACTGGAGGTTCGGGGTGCGACAAAATAAGCTTTCTCCGAATAAAGCCCAt                                   >  1:724333/1‑250 (MQ=255)                                                                                                                                                                                                    aaCTCACATTTCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTCGGCGGTAACGGGGCGGGGAAATCCACGCTGATGAAAATTATCGCCGGCATTCAGCCGCAAGATAGCGGAGAACTGGAgg                                                                             >  1:860682/1‑203 (MQ=255)                                                                                                                                                                                                      cTCACATTTCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTCGGCGGTAACGGGGCGGGGAAATCCACGCTGATGAAAATTATCGCCGGCATTCAGCCGCAAGATAGCGGAGAACTGGAGGTTCGGGGTGCGACAAAATAAGCTTtctc                                                 >  1:827772/1‑229 (MQ=255)                                                                                                                                                                                                      cTCACATTTCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTCGGCGGTAACGGGGCGGGGAAATCCACGCTGATGAAAATTATCGCCGGCATTCAGCCGCAAGATAGCGGAGAACTGGAGGTTCGGGGTGCGACAAAATAAGCTTTCTCCGAATAAAGCCCATCAGCTAgg                           >  1:589247/1‑251 (MQ=255)                                                                                                                                                                                                      cTCACATTTCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTCGGCGGTAACGGGGCGGGGAAATCCACGCTGATGAAAATTATCGCCGGCATTCAGCCGCAAGATAGCGGAGAACTGGAGGTTCGGGGTGCGACAAAATAAGCTTTCTCCGAATAAAGCCCATCAGCTAgg                           >  1:435380/1‑251 (MQ=255)                                                                                                                                                                                                       tCACATTTCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTCGGCGGTAACGGGGCGGGGAAATCCACGCTGATGAAAATTATCGCCGGCATTCAGCCGCAAGATAGCGGAGAACTGGAGGTTCGGGGTGCGACAAAATAAGCTTTCTCCGAATAAAGCCCATCAGCTAGGt                          <  1:484737/251‑1 (MQ=255)                                                                                                                                                                                                            tttCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTCGGCGGTAACGGGGCGGGGAAATCCACGCTGATGAAAATTATCGCCGGCATTCAGCCGCAAGATAGCGGAGAACTGGAGGTTCGGGGTGCGACAAAATAAGCTTTCTCCGAATAAAGCCCATCAGCTAGGt                          >  1:565714/1‑246 (MQ=255)                                                                                                                                                                                                              tCAGATCCAAAATGCTTAATATCCACTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTCGGCGGTAACGGGGCGGGGAAATCCACGCTGATGAAAATTATCGCCGGCATTCAGCCGCAAGATAGCGGAGAACTGGAGGTTCGGGGTGCGACAAAATAAGCTTTCTCCGAATAAAGCCCATCAGCTAGGtatttatt                   <  1:643449/251‑1 (MQ=255)                                                                                                                                                                                                                 gagCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGAt                                                                                                                                                                                                >  1:551551/1‑75 (MQ=255)                                                                                                                                                                                                                    ccAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTCGGCGGTAACGGGGCGGGGAAATCCACGCTGATGAAAATTATCGCCGGCATTCAGCCGCAAGATAGCGGAGAACTGGAGGTTCGGGGTGCGACAAAATAAGCTTTCTCCGAATAAAGCCCATCAGCTAGGTATTTATTTGGTGc             <  1:753074/251‑1 (MQ=255)                                                                                                                                                                                                                      aaaaTGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTCGGCGGTAACGGGGCGGGGAAATCCATGCTGATGGAAATTATCGCCGGCATTCAGCCGCAAGATAGCGGAGAACTGGAGGTTCGGGGTGCGACAAAATAAGCTTTCTCCGAATAAAGCCCCTCAGCTAGGTATTTATTTGGTGCCg           >  1:88713/1‑251 (MQ=255)                                                                                                                                                                                                                         aTGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTCGGCGGTAACGGGGCGGGGAAATCCACGCTGATGAAAATTATCGCCGGCATTCAGCCGCAAGATAGCGGAGAACTGGAGGTTCGGGGTGCGACAAAATAAGCTTTCTCCGAATAAAGCCCATCAGCTAGGTATTTATTTGGTGCCGCAg        >  1:172804/1‑251 (MQ=255)                                                                                                                                                                                                                           gCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTCGGCGGTAACGGGGCGGGGAAATCCACGCTGATGAAAATTATCGCCGGCATTCAGCCGCAAGATAGCGGAGAACTGGAGGTTCGGGGTGCGACAAAATAAGCTTTCTCCGAATAAAGCCCATCAg                                <  1:858088/225‑1 (MQ=255)                                                                                                                                                                                                                           gCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTCGGCGGTAACGGGGCGGGGAAATCCACGCTGATGAAAATTATCGCCGGCATTCAGCCGCAAGATAGCGGAGAACTGGAGGTTCGGGGTGCGACAAAATAAGCTTTCTCCGAATAAAGCCCATCAGCTAGGTATTTATTTGGTGCCGCAGGa      >  1:284551/1‑251 (MQ=255)                                                                                                                                                                                                                             ttAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTCGGCGGTAACGGGGCGGGGAAATCCACGCTGATGAAAATTATCGCCGGCATTCAGCCGCAAGATAGCGGAGAACTGGAGGTTCGGGGTGCGACAAAATAAGCTTTCTCCGAATAAAGCCCATCAGCTAGGTATTTATTTGGTGCCGCAGGAAc    <  1:527610/251‑1 (MQ=255)                                                                                                                                                                                                                              tAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTCGGCGGTAACGGGGCGGGGAAATCCACGCTGATGAAAATTATCGCCGGCATTCAGCCGCAAGATAGCGGAGAACTGGAGGTTCGGGGTGCGACAAAATAAGCTTTCTCCGAATAAAGc                                       >  1:530760/1‑215 (MQ=255)                                                                                                                                                                                                                                atatCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTCGGCGGTAACGGGGCGGGGAAATCCACGCTGATGAAAATTATCGCCGGCATTCAGCCGCAAGATAGCGGAGAACTGGAGGTTCGGGGTGCGACAAAATAAGCTTTCTCCGAATAAAGCCCATCAGCTAGGTATTTATTTGGTGCCGCAGGAACCg  >  1:835907/1‑250 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                  TATCAATTGTTCTTACCGTTTTTTTGTAAAAGCATGATGGAGATCACTTTTTTAAAAATTAAAGGCGATTTTTAGATAATCTTATCCAGATTTGTGATCTAGTTCTCAGAAATTTTGTGCATTCCTGACTAGTATTTTGAAAAAATGTTTTTTTTAAATACATTTGTTCATTCTATGCGGCAGGTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAATATCCGCCCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTCGGCGGTAACGGGGCGGGGAAATCCACGCTGATGAAAATTATCGCCGGCATTCAGCCGCAAGATAGCGGAGAACTGGAGGTTCGGGGTGCGACAAAATAAGCTTTCTCCGAATAAAGCCCATCAGCTAGGTATTTATTTGGTGCCGCAGGAACCG  >  1/1190598‑1191070

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑