Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,190,736	G→A	intergenic (+31156/+230201)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,190,736	0	G	A	100.0%	62.1 / NA	24	intergenic (+31156/+230201)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/13);  total (11/13)

GATTATCCGTCATTAATTCGTTCATATGATTTATTTGTTTTTATTGATTTTGGGTATTATTCATTTGTTTTTTATTATTATCAATTGTTCTTACCGTTTTTTTGTAAAAGCATGATGGAGATCACTTTTTTAAAAATTAAAGGCGATTTTTAGATAATCTTATCCAGATTTGTGATCTAGTTCTCAGAAATTTTGTGCATTCCTGACTAGTATTTTGAAAAAATGTTTTTTTTAAATACATTTGTTCATTCTATGCGGCAGGTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAATATCCGCCCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTCGGCGGTAACGGGGCGGGGAAATCCACGCTGATGAAAATTATCGCCGGCATTCA  >  1/1190520‑1190969                                                                                                                                                                                                                         |                                                                                                                                                                                                                                          gATTATCCGTCATTAATTCGTTCATATGATTTATTTGTTTTTATTGATTTTGGGTATTATTCATTTGTTTTTTATTATTATCAATTGTTCTTACCGTTTTTTTGTAAAAGCATGATGGAGATCAC‑TTTTTAAAAATTAAAGGCGATTTTTAGATAATCTTATCCAGATTTGTGATCTAGTTCTCAGAAATTTTGTGCATTCCTGACTAGTATTTTAAAAAAATGTTTTTTTTAAATa                                                                                                                                                                                                                      <  1:176639/237‑1 (MQ=255)          tCATTAATTCGTTCATATGATTTATTTGTTTTTATTGATTTTGGGTATTATTCATTTGTTTTTTATTATTATCAATTGTTCTTACCGTTTTTTTGTAAAAGCATGATGGAGATCACTTTTTTAAAAATTAAAGGCGATTTTTAGATAATCTTATCCAGATTTGTGATCTAGTTCTCAGAAATTTTGTGCATTCCTGACTAGTATTTTAAAAAAATGTTTTTTTTAAATACATTTGTTCATTCTATGCGGc                                                                                                                                                                                                 <  1:645428/250‑1 (MQ=255)                   cGTTCATATGATTTATTTGTTTTTATTGATTTTGGGTATTATTCATTTGTTTTTTATTATTATCAATTGTTCTTACCGTTTTTTTGTAAAAGCATGATGGAGATCACTTTTTTAAAAATTAAAGGCGATTTTTAGATAATCTTATCCAGATTTGTGATCTAGTTCTCAGAAATTTTGTGCATTCCTGACTAGTATTTTAAAAAAATGTTTTTTTTAAATACATTTGTTCATTCTATGCGGCAGGTAAAAGa                                                                                                                                                                                       <  1:809428/251‑1 (MQ=255)                          atGATTTATTTGTTTTTATTGATTTTGGGTATTATTCATTTGTTTTTTATTATTATCAATTGTTCTTACCGTTTTTTTGTAAAAGCATGATGGAGATCACTTTTTTAAAAATTAAAGGCGATTTTTAGATAATCTTATCCAGATTTGTGATCTAGTTCTCAGAAATTTTGTGCATTCCTGACTAGTATTTTAAAAAAATGTTTTTTTTAAATACATTTGTTCATTCTATGCGGCAGGTAAAAGATGCAAAc                                                                                                                                                                                <  1:724181/251‑1 (MQ=255)                           tGATTTATTTGTTTTTATTGATTTTGGGTATTATTCATTTGTTTTTTATTATTATCAATTGTTCTTACCGTTTTTTTGTAAAAGCATGATGGAGATCACTTTTTTAAAAATTAAAGGCGATTTTTAGATAATCTTATCCAGATTTGTGATCTAGTTCTCAGAAATTTTGTGCATTCCTGACTAGTATTTTAAAAAAATGTTTTTTTTAAATACATTTGTTCATTc                                                                                                                                                                                                         >  1:653878/1‑225 (MQ=255)                                                              cATTTGTTTTTTATTATTATCAATTGTTCTTACCGTTTTTTTGTAAAAGCATGATGGAGATCACTTTTTTAAAAATTAAAGGCGATTTTTAGATAATCTTATCCAGATTTGTGATCTAGTTCTCAGAAATTTTGTGCATTCCTGACTAGTATTTTAAAAAAATGTTTTTTTTAAATACATTTGTTCATTCTATGCGGCAGGTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAATATCCGctcg                                                                                                                                            >  1:296625/1‑248 (MQ=255)                                                                               tatCAATTGTTCTTACCGTTTTTTTGTAAAAGCATGATGGAGATCACTTTTTTAAAAATTAAAGGCGATTTTTAGATAATCTTATCCAGATTTGTGATCTAGTTCTCAGAAATTTTGTGCATTCCTGACTAGTATTTTAAAAAAATGTTTTTTTTAAATACATTTGTTCATTCTATGCGGCAGGTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGt                                                                                                                           >  1:840746/1‑251 (MQ=255)                                                                                  cAATTGTTCTTACCGTTTTTTTGTAAAAGCATGATGGAGATCACTTTTTTAAAAATTAAAGGCGATTTTTAGATAATCTTATCCAGATTTGTGATCTAGTTCTCAGAAATTTTGTGCATTCCTGACTAGTATTTTAAAAAAATGTTTTTTTTAAATACATTTGTTCATTCTATGCGGCAGGTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAatat                                                                                                                                                   <  1:743085/224‑1 (MQ=255)                                                                                  cAATTGTTCTTACCGTTTTTTTGTAAAAGCATGATGGAGATCACTTTTTTAAAAACTAAAGGCGATTTTTAGATAATCTTATCCAGATTTGTGATCTAGTTCTCAGAAATTTTGTGCATTCCTGACTAGTATTTTAAAAAAATGTTTTTTTTAAATACATTTGTTCATTCTATGCGGCAGGTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAATATc                                                                                                                                                  <  1:454612/225‑1 (MQ=255)                                                                                                                                       aaTTAAAGGCGATTTTTAGATAATCTTATCCAGATTTGTGATCTAGTTCTCAGAAATTTTGTGCATTCCTGACTAGTATTTTAAAAAAATGTTTTTTTTAAATACATTTGTTCATTCTATGCGGCAGGTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTg                                                                   >  1:328985/1‑251 (MQ=255)                                                                                                                                               gCGATTTTTAGATAATCTTATCCAGATTTGTGATCTAGTTCTCAGAAATTTTGTGCATTCCTGACTAGTATTTTAAAAAAATGTTTTTTTTAAATACATTTGTTCATTCTATGCGGCAGGTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTgcacgcac                                                            <  1:837841/250‑1 (MQ=255)                                                                                                                                                   tttttAGATAATCTTATCCAGATTTGTGATCTAGTTCTCAGAAATTTTGTGCATTCCTGACTAGTATTTTAAAAAAATGTTTTTTTTAAATACATTTGTTCATTCTATGCGGCAGGTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGc                                                                             <  1:391742/229‑1 (MQ=255)                                                                                                                                                      ttAGATAATCTTATCCAGATTTGTGATCTAGTTCTCAGAAATTTTGTGCATTCCTGACTAGTATTTTAAAAAAATGTTTTTTTTAAATACATTTGTTc                                                                                                                                                                                                             <  1:99412/98‑1 (MQ=255)                                                                                                                                                             aTCTTATCCAGATTTGTGATCTAGTTCTCAGAAATTTTGTGCATTCCTGACTAGTATTTTAAAAAAATGTTTTTTTTAAATACATTTGTTCATTCTATGCGGCAGGTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAatat                                                                                                                                                   <  1:680055/149‑1 (MQ=255)                                                                                                                                                             aTCTTATCCAGATTTGTGATCTAGTTCTCAGAAATTTTGTGCATTCCTGACTAGTATTTTAAAAAAATGTTTTTTTTAAATACATTTGTTCATTCTATGCGGCAGGTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGcc                                                                                                 <  1:208877/199‑1 (MQ=255)                                                                                                                                                             aTCTTATCCAGATTTGTGATCTAGTTCTCAGAAATTTTGTGCATTCCTGACTAGTATTTTAAAAAAATGTTTTTTTTAAATACATTTGTTCATTCTATGCGGCAGGTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTcggcgg                                                  >  1:348258/1‑246 (MQ=255)                                                                                                                                                                  aTCCAGATTTGTGATCTAGTTCTCAGAAATTTTGTGCATTCCTGACTAGTATTTTAAAAAAATGTTTTTTTTAAATACATTTGTTCATTCTATGCGGCAGGTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTCGGCGGTAAcggggcg                                        >  1:486868/1‑251 (MQ=255)                                                                                                                                                                       gATTTGTGATCTAGTTCTCAGAAATTTTGTGCATTCCTGACTAGTATTTTAAAAAAATGTTTTTTTTAAATACATTTGTTCATTCTATGCGGCAGGTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTCGGCGGTAACGGGGCGGGGaa                                   >  1:770588/1‑251 (MQ=255)                                                                                                                                                                          ttGTGATCTAGTTCTCAGAAATTTTGTGCATTCCTGACTAGTATTTTAAAAAAATGTTTTTTTTAAATACATTTGTTCATTCTATGCGGCAGGTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTCGGCGGTAACGGGGCGGGGAAAt                                 >  1:659379/1‑250 (MQ=255)                                                                                                                                                                                                        ttCCTGACTAGTATTTTAAAAAAATGTTTTTTTTAAATACATTTGTTCATTCTATGCGGCAGGTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTCGGCGGTAACGGGGCGGGGAAATCCACGCTGATGAAAATTATCGCCGGCATTc   <  1:880307/250‑1 (MQ=255)                                                                                                                                                                                                        ttCCTGACTAGTATTTTAAAAAAATGTTTTTTTTAAATACATTTGTTCATTCTATGCGGCAGGTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTCGGCGGTAACGGGGCGGGGAAATCCACGCTGATGAAAATTATCGCCGGCATTCa  >  1:741126/1‑251 (MQ=255)                                                                                                                                                                                                          ccTGACTAGTATTTTAAAAAAATGTTTTTTTTAAATACATTTGTTCATTCTATGCGGCAGGTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTgcacgca                                                             <  1:151273/190‑1 (MQ=255)                                                                                                                                                                                                             gACTAGTATTTTAAAAAAATGTTTTTTTTAAATACATTTGTTCATTCTATGCGGCAGGTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTCGGCGGTAAcgggg                                          >  1:822660/1‑206 (MQ=255)                                                                                                                                                                                                             gACTAGTATTTTAAAAAAATGTTTTTTTTAAATACATTTGTTCATTCTATGCGGCAGGTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAATATCCGCTCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTCGGCGGTAACGGGGCGGGGAAATCCACGCTGATGAAAAtt                >  1:312919/1‑232 (MQ=255)                                                                                                                                                                                                                         |                                                                                                                                                                                                                                          GATTATCCGTCATTAATTCGTTCATATGATTTATTTGTTTTTATTGATTTTGGGTATTATTCATTTGTTTTTTATTATTATCAATTGTTCTTACCGTTTTTTTGTAAAAGCATGATGGAGATCACTTTTTTAAAAATTAAAGGCGATTTTTAGATAATCTTATCCAGATTTGTGATCTAGTTCTCAGAAATTTTGTGCATTCCTGACTAGTATTTTGAAAAAATGTTTTTTTTAAATACATTTGTTCATTCTATGCGGCAGGTAAAAGATGCAAACTCACATTTCAGAGCCAAAATGCTTAATATCCGCCCGAAACATCGGTAAGTCGTTTTCCGGCGTGGAAGTGCTGAAAGCCATTGATTTTTCCTTATATGCCGGTGAAGTGCACGCACTGCTCGGCGGTAACGGGGCGGGGAAATCCACGCTGATGAAAATTATCGCCGGCATTCA  >  1/1190520‑1190969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑