Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,188,118	A→G	intergenic (+28538/+232819)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,188,118	0	A	G	100.0%	167.6 / NA	63	intergenic (+28538/+232819)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/36);  total (27/36)

GGGTTTTACCGATGTTTTTTACGGTATATCAACAAAAAATCGTAGAGAGTTGTCCCCCCTACGATTTTTTTATCGTTCGCTACAACCCTGGTGCCTTCCACAAAGAAGTAGTTAATCCGCTATCAACCAGTTGTAATTGTTTTTTATAAGTATCTGCCCATTGTGCTTTGTGAGCTTGGTATAAAGCATAGTTATCGGGATTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTCCCCCACCTCGGCTAAGGTCGAATAAATGCCGACTCCGACGCCTGCGGCAATGGCACAACCCAGCGCTGTAGCTTCTTTGACAATCGGAATGTTTACCGTTAATCCGGTGACATCGGCTAAGATCTGGCTCCATAATTTGCCTTTAGACCCGCCGCCGGCAAAGACAATGGAGGTTAATTTAACTCCTGAGAATTTTTGCACCTGATCCAAATTGCAGGAAGAAACGATAGCAGCATTTTCTTCCAAGG  >  1/1187876‑1188361                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    gggTTTTACCGATGTTTTTTACGGTATATCAACAAAAAATCGTAGAGAGTTGTCCCCCCTACGATTTTTTTATCATTCGCTACAACCCTGGTGCCTTCTACAAAGAAGTAGTTAATCCGCTATCAACCAGTTGTAATTGTTTTTTATAAATATCTGCCCATTGTGCTTTGTGAGCTTGGTATAAAGCATAGTTATCGGGATTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTTCCCCGCCTCgg                                                                                                                                                                                                                                               >  1:303988/1‑249 (MQ=255) gggTTTTACCGATGTTTTTTACGGTATATCAACAAAAAATCGTAGAGAGTTGTCCCCCCTACGATTTTTTTATCATTCGCTACAACCCTGGTGCCTTCTACAAAGAAGTAGTTAATCCGCTATCAACCAGTTGTAATTGTTTTTTATAAATATCTGCCCATTGTGCTTTGTGAGCTTGGTATAAAGCATAGTTATCGGGATTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTTCCCCGCCTCgg                                                                                                                                                                                                                                               >  1:822620/1‑249 (MQ=255) gggTTTTACCGATGTTTTTTACGGTATATCAACAAAAAATCGTAGAGAGTTGTCCCCCCTACGATTTTTTTATCATTCGCTACAACCCTGGTGCCTTCTACAAAGAAGTAGTTAATCCGCTATCAACCAGTTGTAATTGTTTTTTATAAATATCTGCCCATTGTGCTTTGTGAGCTTGGTATAAAGCATAGTTATCGGGATTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTTCCCCGCCTCgg                                                                                                                                                                                                                                               >  1:548765/1‑249 (MQ=255)      ttACCGATGTTTTTTACGGTATATCAACAAAAAATCGTAGAGAGTTGTCCCCCCTACGATTTTTTTATCATTCGCTACAACCCTGGTGCCTTCTACAAAGAAGTAGTTAATCCGCTATCAACCAGTTGTAATTGTTTTTTATAAATATCTGCCCATTGTGCTTTGTGAGCTTGGTATAAAGCATAGTTATCGGGATTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTTCCCCGCCTCGGCTAAGGt                                                                                                                                                                                                                                        <  1:538333/251‑1 (MQ=255)            aTGTTTTTTACGGTATATCAACAAAAAATCGTAGAGAGTTGTCCCCCCTACGATTTTTTTATCATTCGCTACAACCCTGGTGCCTTCTACAAAGAAGTAGTTAATCCGCTATCAACCAGTTGTAATTGTTTTTTATAAATATCTGCCCATTGTGCTTTGTGAGCTTGGTATAAAGCATAGTTATCGGGATTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTTCCCCGCCTCGGCTAAGGTCggat                                                                                                                                                                                                                                   <  1:113542/250‑4 (MQ=255)            aTGTTTTTTACGGTATATCAACAAAAAATCGTAGAGAGTTGTCCCCCCTACGATTTTTTTATCATTCGCTACAACCCTGGTGCCTTCTACAAAGAAGTAGTTAATCCGCTATCAACCAGTTGTAATTGTTTTTTATAAATATCTGCCCATTGTGCTTTGTGAGCTTGGTATAAAGCATAGTTATCGGGATTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTTCCCCGCCTCGGCTAAGGTCGGATa                                                                                                                                                                                                                                  <  1:33208/251‑1 (MQ=255)                 ttttACGGTATATCAACAAAAAATCGTAGAGAGTTGTCCCCCCTACGATTTTTTTATCATTCGCTACAACCCTGGTGCCTTCTACAAAGAAGTAGTTAATCCGCTATCAACCAGTTGTAATTGTTTTTTATAAATATCTGCCCATTGTGCTTTGTGAGCTTGGTATAAAGCATAGTTATCGGGATTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTTCCCCGCCTCGGCTAAGGTCGGATAAATGc                                                                                                                                                                                                                             <  1:194914/251‑1 (MQ=255)                 ttttACGGTATATCAACAAAAAATCGTAGAGAGTTGTCCCCCATACGATTTTTTTATCATTCGCTACAACCCTGGTGCCTTCTACAAAGAAGTAGTTAATCCGCTATCAACCAGTTGTAATTGTTTTTTATAAATATCTGCACTTTGTGCTTTGTGAGTTTGGTATAAAGCATAGTTATCGGGATTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTTCCCCGCCTCGGCTAAGGTCGGATAAATGc                                                                                                                                                                                                                             <  1:551592/251‑1 (MQ=255)                        gTATATCAACAAAAAATCGTAGAGAGTTGTCCCCCCTACGATTTTTTTATCATTCGCTACAACCCTGGTGCCTTCTACAAAGAAGTAGTTAATCCGCTATCAACCAGTTGTAATTGTTTTTTATAAATATCTGCCCATTGTGCTTTGTGAGCTTGGTATAAAGCATAGTTATCGGGATTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTTCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTc                                                                                                                                                                                                                       >  1:292046/1‑250 (MQ=255)                        gTATATCAACAAAAAATCGTAGAGAGTTGTCCCCCCTACGATTTTTTTATCATTCACTACAACCCTGGTGCCTTCTACAAAGAAGTAGTTAATCCGCTATCAACCAGTTGTAATTGTTTTTTATAAATATCTGCCCATTGTGCTTTGTGAGCTTGGTATAAAGCATAGTTATCGGGATTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTTCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTc                                                                                                                                                                                                                       >  1:253458/1‑250 (MQ=255)                                   aaaaaTCGTAGAGAGTTGTCCCCCCTACGATTTTTTTATCATTCGCTACGCCCCTGGTGCTTTCTACAAAGAAGTAGTTAATCCGCTATCAACCTGTTGTAATTGTTTTTTATAAATATCTGCCCATTGTGCTTTGTGAGCTTGGTATAAAGCATAGTTATCGGGTTTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTTCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTCCGACGCCTGCgg                                                                                                                                                                                                           <  1:336902/251‑1 (MQ=255)                                                      ccccccTACGATTTTTTTATCATTCGCTACAACCCTGGTGCCTTCTACAAAGAAGTAGTTAATCCGCTATCAACCAGTTGTAATTGTTTTTTATAAATATCTGCCCATTGTGCTTTGTGAGCTTGGTATAAAGCATAGTTATCGGGATTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTTCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTCCGACGCCTGCGGCAATGGCACAACCCAgcgc                                                                                                                                                                                        <  1:831482/251‑1 (MQ=255)                                                                      ttATCATTCGCTACAACCCTGGTGCCTTCTACAAAGAAGTAGTTAATCCGCTATCAACCAGTTGTAATTGTTTTTTATAAATATCTGCCCATTGTGCTTTGTGAGCTTGGTATAAAGCATAGTTATCGGGATTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTTCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTCCGACGCCTGCGGCAATGGCACAACCCAGCGCTGTAGCTTCTTTGACa                                                                                                                                                                        <  1:678313/251‑1 (MQ=255)                                                                                cTACAACCCTGGTGCCTTCTACAAAGAAGTAGTTAATCCGCTATCAACCAGTTGTAATTGTTTTTTATAAATATCTGCCCATTGTGCTTTGTGAGCTTGGTATAAAGCATAGTTATCGGGATTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTTCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTCCGACGCCTGCGGCAATGGCACAACCCAGCGCTGTAGCTTCTTTGACAATCGGAATGt                                                                                                                                                              >  1:712643/1‑251 (MQ=255)                                                                                       ccTGGTGCCTTCTACAAAGAAGTAGTTAATCCGCTATCAACCAGTTGTAATTGTTTTTTATAAATATCTGCCCTTTGTGCTTTGTGAGCTTGGTATAAAGCATAGTTATCGGGATTCGGTTGATGTTGCCGTTCAAATTTAACCATTTTTTTCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTCCGACGCCTGCGGCAATGGCACAACCCAGCGCTGTAGCTTCTTTGACAATCGGAATGTTTACCGt                                                                                                                                                       <  1:599234/251‑1 (MQ=255)                                                                                            tGCCTTCTACAAAGAAGTAGTTAATCCGCTATCAACCAGTTGTAATTGTTTTTTATAAATATCTGCCCATTGTGCTTTGTGAGCTGGGAATAAAGCATAGTTATCGGGATTCGGTTGATGTTGCCGTTAAAATTTAACCAGTTTTTTCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTCCGACGCCTGCGGCAATGGCACAACCCAGCGCTGTAGCTTCTTTGACAATCGGAATGTTTACCGTTAATc                                                                                                                                                  <  1:23436/251‑1 (MQ=255)                                                                                             gCCTTCTACAAAGAAGTAGTTAATCCGCTATCAACCAGTTGTAATTGTTTTTTATAAATATCTGCCCATTGTGCTTTGTGAGCTTGGTATAAAGCATAGTTATCGGGATTCGGTTGATGTTGCCGTTCAAACTTAACCAGTTTTTTCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTCCGACGCCTGCGGCAATGGCACAACCCAGCGCTGTAGCTTCTTTGACAATCGGAATGTTTACCGTTAATc                                                                                                                                                  >  1:357319/1‑250 (MQ=255)                                                                                                  ctacaAAGAAGTAGTTAATCCGCTATCAACCAGTTGTAATTGTTTTTTATAAATATCTGCCCATTGTGCTTTGTGAGCTTGGTATAAAGCATAGTTATCGGGATTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTTCCCCGCCTCGGCTAAGGTc                                                                                                                                                                                                                                       <  1:410807/158‑1 (MQ=255)                                                                                                  ctacaAAGAAGTAGTTAATCCGCTATCAACCAGTTGTAATTGTTTTTTATAAATATCTGCCCATTGTGCTTTGTGAGCTTGGTATAAAGCATAGTTATCGGGATTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTTCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTCCGACGCCTGCGGCAATGGCACAACCCAgcgc                                                                                                                                                                                        <  1:125175/205‑1 (MQ=255)                                                                                                      aaaGAAGTAGTTAATCCGCTATCACCCAGTTGTAATTGTTTTTTATAAATATCTGCCCATTGTGCTTTGTGAGCTTGGTATAAAGCATAGTTATCGGGATTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTTCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTCCGACGCCTGCGGCAATGGCACAACCCAGCGCTGTAGCTTCTTTGACAATCGGAATGTTTACCGTTAATCCGGTGACATc                                                                                                                                        <  1:99631/251‑1 (MQ=255)                                                                                                             tagtTAATCCGCTATCAACCAGTTGTAATTGTTTTTTATAAATATCTGCCCATTGTGCTTTGTGAGCTTGGTATAAAGCATAGTTATCGGGATTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTTCCCCGCCTCGGCTAAGGTCGGATa                                                                                                                                                                                                                                  <  1:125405/154‑1 (MQ=255)                                                                                                                ttAATCCGCTATCAACCAGTTGTAATTGTTTTTTATAAATATCTGCCCATTGTGCTTTGTGAGCTTGGTATAAAGCATAGTTATCGGGATTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTTCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTCCGACGCCTGCGGCAATGGCACAACCCAGCGCTGTAGCTTCTTTGACAATCGGAATGTTTACCGTTAATCCGGTGACATCGGCTAAGATc                                                                                                                              <  1:775665/251‑1 (MQ=255)                                                                                                                ttAATCCCCTCTCATCCAGTTTTAATTGTTTTTTATAAATATCTGCCCTTTGTGCTTTGTGAGCTGGGTATAAAGCATTGTTATCGGGATTGGGTTCATGTTGCCGTTCAAATTTAACTAATTTTTTCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTCCGACGCCTGCGGCAATGGCACAACCCAGCGCTGTAGCTTCTTTGACAATCGGAATGTTTACCGTTAATCCGGTGACATCGGCTAAGAt                                                                                                                               <  1:784371/250‑1 (MQ=255)                                                                                                                      cGCTATCAACCAGTTGTAATTTTTTTTTATAAATATCTGCCCATTGTGCTTTGTGAGCTTGGTATAAAGCATAGTTATCGGGATTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTCCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTCCGACGCCTGCGGCAATGGCACAACCCAGCGCTGTAGCTTCTTTGACAATCGGAATGTTTACCGTTAATCCGGTGACATCGGCTAAGATCTGGCTc                                                                                                                        <  1:201116/251‑1 (MQ=255)                                                                                                                        cTATCAACCAGTTGTAATTGTTTTTTATAAATATCTGCCCATTGTGCTTTGTGAGCTTGGTATAAAGCATAGTTATCGGGATTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTTCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTCCGACGCCTGCGGCa                                                                                                                                                                                                         >  1:250204/1‑168 (MQ=255)                                                                                                                               ccAGTTGTAATTGTTTTTTATAAATTTCTGCCCATTTTGCTTTTTGAGCTTGGTAAAAACCTTAGTTATCGGAATTCGTTTGATGTTGCCGTTCAAATTTACCCAGTTTTTCCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTCCGACGCCTGCGGCAATGGCACAACCCAGCGCTGTAGCTTCTTTGACAATCGGAATGTTTACCGTTAATCCGGTGACATCGGCTAAGATCTGGCTCCATAATTTg                                                                                                               <  1:78209/251‑1 (MQ=255)                                                                                                                                cAGTTGTAATTGTTTTTTATAAATATCTGCCCATTGTGCTTTGTGAGCTTGGTATAAAGCATAGTCATCGGGATTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTTCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTCCGACGCCTGCGGCAATGGCACAACCCAGCGCTGTAGCTTCTTTGACAATCGGAATGTTTACCGTTAATCCGGTGACATCGGCTAAGATc                                                                                                                              <  1:429467/235‑1 (MQ=255)                                                                                                                                  gTTGTAATTGTTTTTTATAAATATCTGCCCATTGTGCTTTGTGAGCTTGGTATAAAGCATAGTTATCGGGATTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTTCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTCCGACGCCTGCGGCAATGGCACAACCCAGCGCTGTAGCTTCTTTGACAATCGGAATGTTTACCGTTAATCCGGTGACATCGGCTAAGATCTGGCTCCATAATTTGc                                                                                                              >  1:245507/1‑249 (MQ=255)                                                                                                                                        atttttttttATAAATATCTGCCCATTGTGCTTTGTGAGCTTGGTATAAAGCATAGTTATCGGGATTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTCCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTCCGACGCCTGCGGCAATGGCACAACCCAGCGCTGTAGCTTCTTTGACAATCGGAATGTTTACCGTTAATCCGGTGACATCGGCTAAGATCTGGCTCCATAATTTGCCTTTaaac                                                                                                      <  1:871066/247‑4 (MQ=255)                                                                                                                                        aTTGTTTTTTATAAATATCTGCCCATTGAGCTTTGTGAGCTTGGTATAAAGCATAGTTATCGGGATTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTTCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTCCGACGCCTGCGGCAATGGCACAACCCAGCGCTGTAGCTTCTTTGACAATCGGAATGTTTACCGTTAATCCGGTGACATCGGCTAAGATCTGGCTCCATAATTTGCCTTTAGAc                                                                                                      <  1:216211/251‑1 (MQ=255)                                                                                                                                                  ataAATATCTGCCCATTGTGCTTTGTGAGCTTGGTATAAAGCATAGTTATCGGGATTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTTCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTCCGACGCCTGCGGCAATGGCACAAccc                                                                                                                                                                                             <  1:280073/154‑1 (MQ=255)                                                                                                                                                  ataAATATCTGCCCATTGTGCTTTGTGAGCTTGGTATAAAGCATAGTTATCGGGATTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTTCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTCCGACGCCTGCGGCAATGGCACAACCCAGCGCTGTAGCTTCTTTGACAATCGGAATGTTTACCGt                                                                                                                                                       <  1:677111/192‑1 (MQ=255)                                                                                                                                                       tATCTGCCCATTGTGCTTTGTGAGCTTGGTATAAAGCATAGTTATCGGGATTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTTCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTCCGACGCCTGCGGCAATGGCACAACCCAGCGCTGTAGCTTCTTTGACAATCGGAATGTTTACCGTTAATCCGGTGACATCGGCTAAGATCTGGCTCCATAATTTGCCTTTAGACCCGCCGCCGGCAAAg                                                                                       >  1:864446/1‑251 (MQ=255)                                                                                                                                                           tGCCCATTGTGCTTTGTGAGCTTGGTATAAAGCATAGTTATCGGGATTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTTCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTCCGACGCCTGCGGCAATGGCACAACCCAgcgc                                                                                                                                                                                        >  1:135464/1‑150 (MQ=255)                                                                                                                                                            gCCCATTGTGCTTTGTGAGCTTGGTATAAAGCATAGTTATCGGGATTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTTCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTCCGACGCCTGCGGCAATGGCACAACCCAGCGCTGTAGCTTCTTTGACAATCGGAATGTTTACCGTTAATCCGGTGACATCGGCTAAGATCTGGCTCCATAATTTGCCTTTAGACCCGCCGCCGGCAAAGACAAt                                                                                  >  1:824343/1‑251 (MQ=255)                                                                                                                                                              ccATTGTGCTTTGTGAGCTTGGTATAAAGCATAGTTATCGGGATTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTTCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTCCGACGCCTGCGGCAATGGCACAACCCAGCGCTGTAGCTTCTTTGACAATCGGAATGTTTACCGTTAATCCGGTGACATCGGCTAAGATCTGGCt                                                                                                                         >  1:198405/1‑210 (MQ=255)                                                                                                                                                              ccATTGTGCTTTGTGAGCTTGGTATAAAGCATAGTTATCGGGATTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTTCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTCCGACGCCTGCGGCAATGGCACAACCCAGCGCTGTAGCTTCTTTGACAATCGGAATGTTTACCGTTAATCCGGTGACATCGGCTAAGATCTGGCTCCATAATTTGCCTTTAGACCCGCCGCCGGCAAAGACAATgg                                                                                >  1:195019/1‑251 (MQ=255)                                                                                                                                                                   gtgCTTTGTGAGCTTGGTATAAAGCATAGTTATCGGGATTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTTCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTCCGACGCCTGCGGCAATGGCACAACCCAGCGCTGTAGCTTCTTTGACAATCGGAATGTTTACCGt                                                                                                                                                       >  1:346797/1‑175 (MQ=255)                                                                                                                                                                   gtgCTTTGTGAGCTTGGTATAAAGCATAGTTATCGGGATTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTTCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTCCGACGCCTGCGGCAATGGCACAACCCAGCGCTGTAGCTTCTTTGACAATCGGAATGTTTACCGTTAATCCGGTGACATCGGCTAAGATCTGGCTCCATAATTTGCCTTTAGACCCGCCGCCGGCAAAGACAATgg                                                                                >  1:423088/1‑246 (MQ=255)                                                                                                                                                                   gtgCTTTGTGAGCTTGGTATAAAGCATAGTTATCGGGATTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTTCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTCCGACGCCTGCGGCAATGGCACAACCCAGCGCTGTAGCTTCTTTGACAATCGGAATGTTTACCGTTAATCCGGTGACATCGGCTAAGATCTGGCTCCATAATTTGCCTTTAGACCCGCCGCCGGCAAAGACAATGGAGGtt                                                                           >  1:348556/1‑251 (MQ=255)                                                                                                                                                                      cTTTGTGAGCTTGGTATAAAGCATAGTTATCGGGATTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTTCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTCCGACGCCTGCGGCAATGGCACAACCCAGCGCTGTAGCTTCTTTGACAATCGGAATGTTTACCGTTAATCCGGTGACATCGGCTAAGATCTGGCTCCATAATTTGCCTTTAGACCCGCCGCCGGCAAAGACAATGGAGGTTAAt                                                                        <  1:774717/251‑1 (MQ=255)                                                                                                                                                                        ttGTGAGCTTGGTATAAAGCATAGTTATCGGGATTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTTCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTCCGACGCCTGCGGCAATGGCACAACCCAGCGCTGTAGCTTCTTTGACAATCGGAATGTTTACCGTTAATCCGGTGACATCGGCTAAGATCTGGCTCCATAATTTGCCTTTAGACCCGCCGCCGGCAAAGACAATGGAGGTTAAttt                                                                      >  1:859423/1‑251 (MQ=255)                                                                                                                                                                              gCTTGGTATAAAGCATAGTTATCGGGATTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTTCCCCGCCTCGGCTAAGGt                                                                                                                                                                                                                                        <  1:609791/83‑1 (MQ=255)                                                                                                                                                                                 tGGTATAAAGCATAGTTATCGGGATTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTCCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTCCGACGCCTGCGGCAATGGCACAACCCAGCGCTGTAGCTTCTTTGACAATCGGAATGTTTACCGTTAATCCGGTGACATCGGCTAAGATCTGGCTCCATAATTTGCCTTTAGa                                                                                                       <  1:400175/209‑1 (MQ=255)                                                                                                                                                                                   gTATAAAGCATAGTTATCGGGATTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTTCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTCCGACGCCTGCGGCAATGGCACAACCCAGCGCTGTAGCTTCTTTGACAATCGGAATg                                                                                                                                                               <  1:91793/151‑1 (MQ=255)                                                                                                                                                                                   gTATAAAGCATAGTTATCGGGATTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTTCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTCCGACGCCTGCGGCAATGGCACAACCCAGCGCTGTAGCTTCTTTGACAATCGGAATGTTTACCGTTAATc                                                                                                                                                  <  1:90446/164‑1 (MQ=255)                                                                                                                                                                                   gTATAAAGCATAGTTATCGGGATTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTTCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTCCGACGCCTGCGGCAATGGCACAACCCAGCGCTGTAGCTTCTTTGACAATCGGAATGTTTACCGTTAATCCGGTGCCATCGGCTAAGATCTGGCTCCATAATTTGCCTTTAGACCCGCCGCCGGCAAAGACAATGGAGGTTAATTTAACTCCTgaga                                                           >  1:442103/1‑251 (MQ=255)                                                                                                                                                                                   gTATAAAGCATAGTTATCGGGATTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTTCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTCCGACGCCTGCGGCAATGGCACAACCCAGCGCTGTAGCTTCTTTGACAATCGGAATGTTTACCGTTAATCCGGTGACATCGGCTAAGATCTGGCTCCATAATTTGcc                                                                                                             >  1:728920/1‑201 (MQ=255)                                                                                                                                                                                     ataAAGCATAGTTATCGGGATTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTTCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTCCGACGCCTGCGGCAATGGCACAACCCAGCGCTGTAGCTTCTTTGACAATCGGAATGTTTACCGTTAATCCGGTGACATCGGCTAAGATCTGGCTCCATAATTTGCCTTTAGACCCGCCGCCGGCAAAGACAATGGAGGTTAATTTAACTCCTGAGAAt                                                         <  1:584872/251‑1 (MQ=255)                                                                                                                                                                                          gCATAGTTATCGGGATTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTTCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTCCGACGCCTGCGGCAATGGCACAACCCAgcgc                                                                                                                                                                                        <  1:820824/119‑1 (MQ=255)                                                                                                                                                                                          gCATAGTTATCGGGATTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTTCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTCCGACGCCTGCGGCAATGGCACAACCCAGCGCTGTAGCTTCTTTGACAATCGGAATGTTTACCGTTAATCCGGTGACATCGGCTAAGATCTGGCTCCATAATTTGCCTTTAGACCCGCCGCCGGCAAAGACAATGGAGGTTAATTTAACTCCTGAGAATTTTTg                                                    >  1:870574/1‑251 (MQ=255)                                                                                                                                                                                          gCATAGTTATCGGGATTCGGTTGATGTGGCCGTTCAAATTTAACCAGTTTTTTCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTCCGACGCCTGCGGCAATGGCACAACCCAgcgc                                                                                                                                                                                        <  1:823838/119‑1 (MQ=255)                                                                                                                                                                                             tAGTTATCGGGATTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTTCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTCCGACGCCTGCGGCAATGGCACAACCCAGCGCTGTAGCTTCTTTGACAATCGGAATGTTTACCGTTAATCCGGTGACATCGGCTAAGATCTGGCTCCATAATTTGCCTTTAGACCCGCCGCCGGCAAAGACAATGGAGGTTAATTTAACTCCTGAGAATTTTTGCAc                                                 <  1:439114/251‑1 (MQ=255)                                                                                                                                                                                             tAGTTATCGGGATTCGGTTGATGTTGCCGTTAAAATTTAACCAGTTTTTCCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTCCGACGCCTGCGGCAATGGCACAACCCAGCGCTGTAGCTTCTTTGACAATCGGAATGTTTACCGTTAATCCGGTGACATCGGCTAAGATCTGGCTCCATAATTTGCCTTTAGACCCGCCGCCGGCAAAGACAATGGAGGTTAATTTAACTCCTGAGAATTTTTGCAc                                                 <  1:872627/251‑1 (MQ=255)                                                                                                                                                                                               gTTATCGGGATTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTCCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTCCGACGCCTGCGGCAATGGCACAACCCAGCGCTGTAGCTTCTTTGACAATCGGAATGTTTACCGTTAATCCGGTGACATCGGCTAAGAt                                                                                                                               <  1:631501/171‑1 (MQ=255)                                                                                                                                                                                                        aTTCGGTTGATGTTGCCGTTCAAATTTACCCATTTTTTTCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTCCGACGCCTGCGGCAATGGCACAACCCAGCGCTGTAGCTTCTTTGACAATCGGAATGTTTACCGTTAATCCGGTGACATCGGCTAAGATCTGGCTCCATAATTTGCCTTTAGACCCGCCGCCGGCAAAGACAATGGAGGTTAATTTAACTCCTGAGAATTTTTGCACCTGATCCAAAt                                      <  1:598396/251‑1 (MQ=255)                                                                                                                                                                                                        aTTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTTCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTCCGACGCCTGCGGCAATGGCACAACCCAGCGCTGTAGCTTCTTTGACAATCGGAATGTTTACCGTTAATCCGGTGACATCGGCTAAGATCTGGCTCCATAATTTGCCTTTAGACCCGCCGCCGGCAAAGACAATGGAGGTTAATTTAACTCCTGAGAATTTTTGCACCTGATCCAAAt                                      >  1:363283/1‑251 (MQ=255)                                                                                                                                                                                                            ggTTGATGTTGCCGTTCAAATTTAACCAGTTTTTTCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTCCGACGCCTGCGGCAATGGCACAACCCAGCGCTGTAGCTTCTTTGACAATCGGAATGTTTACCGTTAATCCGGTGACATCGGCTAAGATCTGGCTCCATAATTTGCCTTTAGACCCGCCGCCGGCAAAGAc                                                                                     >  1:407676/1‑200 (MQ=255)                                                                                                                                                                                                             gTTGATGTTGCCGTTCAAATTTAACCAGTTTTTTCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTCCGACGCCTGCGGCAATGGCACAACCCAgcgc                                                                                                                                                                                        >  1:652220/1‑100 (MQ=255)                                                                                                                                                                                                                              aaTTTAACCAGTTTTTTCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTCCGACGCCTGCGGCAATGGCACAACCCAGCGCTGTAGCTTCTTTGACAATCGGAATGTTTACCGTTAATCCGGTGACATCGGCTAAGATCTGGCTCCATAATTTGCCTTTAGACCCGCCGCCGGCAAAGAc                                                                                     >  1:158142/1‑182 (MQ=255)                                                                                                                                                                                                                              aaTTTAACCAGTTTTTTCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTCCGACGCCTGCGGCAATGGCACAACCCAGCGCTGTAGCTTCTTTGACAATCGGAATGTTTACCGTTAATCCGGTGACATCGGCTAAGATCTGGCTCCATAATTTGCCTTTAGACCCGCCGCCGGCAAAGACAATGGAGGTTAATTTAACTCCTGAGAATTTTTGCACCTGATCCAAATTGCAGgaagaa                           >  1:482650/1‑240 (MQ=255)                                                                                                                                                                                                                                        gTTTTTTCCCCGCCTCGGCTAAGGTCGGATAAATGCCGACTCCGACGCCTGCGGCAATGGCACAACCCAGCGCTGTAGCTTCTTTGACAATCGGAATGTTTACCGTTAATCCGGTGACATCGGCTAAGATCTGGCTCCATAATTTGCCTTTAGACCCGCCGCCGGCAAAGACAATGGAGGTTAATTTAACTCCTGAGAATTTTTGCACCTGATCCAAATTGCAGGAAGAAACGATAGCAGCATTttcttc       >  1:751800/1‑250 (MQ=255)                                                                                                                                                                                                                                            tttccccGCCTCGGCTAAGGTCGGATAAATGCCGACTCCGACGCCTGCGGCAATGGCACAACCCAGCGCTGTAGCTTCTTTGACAATCGGAATGTTTACCGTTAATCCGGTGACATCGGCTAAGATCTGGCTCCATAATTTGCCTTTAGACCCGCCGCCGGCAAAGACAATGGAGGTTAATTTAACTCCTGAGAATTTTTGCACCTGATCCAAATTGCAGGAAGAAACGATAGCAGCATTTTCTTCCAAgg  <  1:414100/248‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                    GGGTTTTACCGATGTTTTTTACGGTATATCAACAAAAAATCGTAGAGAGTTGTCCCCCCTACGATTTTTTTATCGTTCGCTACAACCCTGGTGCCTTCCACAAAGAAGTAGTTAATCCGCTATCAACCAGTTGTAATTGTTTTTTATAAGTATCTGCCCATTGTGCTTTGTGAGCTTGGTATAAAGCATAGTTATCGGGATTCGGTTGATGTTGCCGTTCAAATTTAACCAGTTTTTCCCCCACCTCGGCTAAGGTCGAATAAATGCCGACTCCGACGCCTGCGGCAATGGCACAACCCAGCGCTGTAGCTTCTTTGACAATCGGAATGTTTACCGTTAATCCGGTGACATCGGCTAAGATCTGGCTCCATAATTTGCCTTTAGACCCGCCGCCGGCAAAGACAATGGAGGTTAATTTAACTCCTGAGAATTTTTGCACCTGATCCAAATTGCAGGAAGAAACGATAGCAGCATTTTCTTCCAAGG  >  1/1187876‑1188361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑