Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,184,455	G→A	intergenic (+24875/+236482)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,184,455	0	G	A	100.0%	172.7 / NA	64	intergenic (+24875/+236482)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (38/26);  total (38/26)

GAATTAGAAATTTGCGTTACGCGGAGCACGTGGGAACGGGATCACATCACGAATATTTTGCAACCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACGACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAAC‑GTTCTTCACGTTGCGAGCCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTCACCACAACAGGGGATTTGAAATAATCTTCTGCCAAATAGCGCTCATGTTCGGAGGAAAGGTCAATGCCCCAAGAAACCGGGAATTCAAAGGTTTTACCGGATTTCAGTAAAATCTCAATGGCGTCGGTG  >  1/1184230‑1184695                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 gaATTAGAAATTTGCGTTACGCGGAGCACGTGGGAACGGGATCACATCACGAATATTTTGCAACCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAAt                                                                                                                                                                                                                                       >  1:581780/1‑237 (MQ=255)        aaaTTTGCGTTACGCGGAGCACGTGGGAACGGGATCACATCACGAATATTTTGCAACCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCAcc                                                                                                                                                                                                                                 >  1:806297/1‑236 (MQ=255)                        gAGCACGTGGGAACGGGATCACATCACGAATATTTTGCACCCCTGTCACATAAACAATTAAACGTTCAAACCCCAAGCCAAAGCCGGAAGGCGGACCCGTGCCGTAACACCGCAAATCACGATACCACCAATAATCTCCCGGATTTAGCCCCATTCCCGCCATGCGTTTGTCTAATACGTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCAcc                                                                                                                                                                                                                                 <  1:390489/220‑1 (MQ=255)                        gAGCACGTGGGAACGGGATCACATCACGAATATTTTGCAACCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCa                                                                                                                                                                                                  >  1:157029/1‑251 (MQ=255)                             cGTGGGAACGGGATCACATCACGAATATTTTGCAACCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGt                                                                                                                                                                                             >  1:197478/1‑251 (MQ=255)                                   aaCGGGTTCACATCCCGAATATTTTGCACCCCTGTCACAAAAACAATTAAACTTAAAAACCCCAAGCCAAAGCCGAAATGCGGACCCGTGCCATAACAACGAAAATCACGATCCCCCCAATAATCTTCCGGATTTGGCCCCATTCCCGCCATGCTTTTGTCTAATCCGTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTcc                                                                                                                                                                                       <  1:320376/251‑1 (MQ=255)                                       ggATCACATCACGAATATTTTGCACCCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGACCCGTGCCGTAACAACGCAAATCACGATCCCACCAATAATCTCCCGGATTTAGCCCCATTCCCGCCATGCGTTTGTCTAATCCGTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTc                                                                                                                                                                                        <  1:15484/246‑1 (MQ=255)                                       ggATCACATCACGAATATTTTGCAACCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCAc                                                                                                                                                                                                                                  >  1:142697/1‑204 (MQ=255)                                       ggATCACATCACGAATATTTTGCAACCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCACCaa                                                                                                                                                                                                                               >  1:11730/1‑207 (MQ=255)                                                cacGAATTTTTTGAAACCCTGTCACATAAACAATTAACCGTTAAAAGCCCAAGCCAAAGCCGGAATGCGGACCCGTGCCGTAACAACGCAAACCACGATCCCCCCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTc                                                                                                                                                                                        <  1:387449/237‑1 (MQ=255)                                                cacGAATATTTTGCAACCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAg                                                                                                                                                                          >  1:476432/1‑251 (MQ=255)                                                cacGAATATTTTGCAACCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAg                                                                                                                                                                          >  1:355217/1‑251 (MQ=255)                                                           tGCAACCCTGTCACAAAAAAAATTAAACGTTAAAAGCCCAAGCAAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATCCGTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGaa                                                                                                                                                               <  1:91117/251‑1 (MQ=255)                                                               aCCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAgg                                                                                                                                                             >  1:818436/1‑249 (MQ=255)                                                                    gTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAAt                                                                                                                                                      >  1:545296/1‑251 (MQ=255)                                                                     tCACAAAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGACCCGGGCCGTAACACCGCAAATCACGATCCTCCCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATCCGTCTAAAC‑GTTCTTCACGTGGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAAt                                                                                                                                                      <  1:426404/250‑1 (MQ=255)                                                                      cacaTAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAAttt                                                                                                                                                    >  1:397338/1‑251 (MQ=255)                                                                      cacaTAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAAttt                                                                                                                                                    >  1:195012/1‑251 (MQ=255)                                                                              cAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGACCCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATCCGTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGaa                                                                                                                                                               <  1:872604/232‑1 (MQ=255)                                                                              cAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGa                                                                                                                                            >  1:346762/1‑251 (MQ=255)                                                                              cAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGa                                                                                                                                            >  1:670036/1‑251 (MQ=255)                                                                                       gTTCAAAGCCAAAGCAAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACAAAAAATCTCCGGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAAC‑GTTCTTCGCGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGtttt                                                                                                                                     <  1:716542/249‑1 (MQ=255)                                                                                        ttCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAg                                                                                                                                                                 >  1:639203/1‑220 (MQ=255)                                                                                          aaaaGCCCAAGCCAAAGCCGGAATGCGGACCCGTGCCGTAACAACGCAAATCACGATACCCCCAATAATCTTCCGGATTTAGCCCCATTCCCGCCATGCGTTTGTCTAATACGTCTAACC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTcacc                                                                                                                                <  1:418037/250‑1 (MQ=255)                                                                                               cccAAGCAAAAGCCGGAATGCGGAACCGTGCCGTACCAACGCAAACCACGATCCCACAAATAATCTCCCGGATTTAGCCCCATTCCCGCCATGCGTTTGTCTAATACGTCTAAAC‑GTTCTTCCCGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTCACCacaac                                                                                                                           <  1:745855/251‑1 (MQ=255)                                                                                                    gCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTCACCACAACAgggg                                                                                                                      >  1:150810/1‑251 (MQ=255)                                                                                                          gCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTCACCACAACAGGGGATTTGa                                                                                                                >  1:229881/1‑251 (MQ=255)                                                                                                            cGGAATGCGGACCCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAAC‑GTTCTTCACTTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTCACCACAACAGGGGATTTGaaa                                                                                                              <  1:719648/251‑1 (MQ=255)                                                                                                                aTGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAAt                                                                                                                                                      >  1:648823/1‑207 (MQ=255)                                                                                                                aTGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTcacc                                                                                                                                >  1:37358/1‑229 (MQ=255)                                                                                                                aTGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTcac                                                                                                                                 >  1:646891/1‑228 (MQ=255)                                                                                                                 tGCGGACCCGTGCCGTAACACCGCAAATCCCGATACCACCAATAATCTTCCGGATTTAGCCCCATTCCCGCCATGCGTTTGTCTAATCCGTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTCACCACAACAGGGGATTTGAAATAAtc                                                                                                         <  1:209503/251‑1 (MQ=255)                                                                                                                   cGGAGCCGGGCCGAAACAACGCAAATCACGATCCCACAAATAATCTTCCGAATTTAGCCCCATTGCCGCCATGCGTTTGTCAAATCCGTCAAAAC‑TTTCTTCACGTGGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTCACCACAACAGGGGATTTGAAATAAtct                                                                                                        <  1:394106/250‑1 (MQ=255)                                                                                                                       aCCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTCACCACAACAGGGGATTTGAAATAATCTTCTg                                                                                                    >  1:175573/1‑250 (MQ=255)                                                                                                                               gTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTCACCACAACAGGGGATTTGAAATAATCTTCTGCCAAATAg                                                                                            >  1:561997/1‑250 (MQ=255)                                                                                                                                 aacaacgCAAATCACGATCCCACCAATAATCTCCCGGATTTAGCCCCTTTTCCGCCATGCGTTTGTCTAATACGTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTCACCACAACAGGGGATTTGAAATAATCTTCTGCCAAATAgcgc                                                                                         <  1:251019/247‑1 (MQ=255)                                                                                                                                      cgCAAATCACGATACCACAAATAATCTCCCGGATTTAGCCCCATTTCCGCCATGCTTTTGTCTAATACGTCAAAAC‑GTTCTCCCCGTGGCAAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTCACCACAACAGGGGATTTGAAATAATCTTCTGCCAAATAGCGCTCATg                                                                                    <  1:422671/251‑1 (MQ=255)                                                                                                                                            tCACGATACCACCAATAATCTCCCGGATTTAGCCCCTTTTCCGCCATGCGTTTGTCTAATACGTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGa                                                                                                                                                                <  1:335935/169‑1 (MQ=255)                                                                                                                                                 agacaccaaataatCTCCCGGATTTAGCCCCATTTCCGCCTTGTGTTTGTATAAGACGTC‑AAACGGTTTTTCACTTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTCACCACAACAGGGGATTTGAAATAATCTTCTGCCAAATAGCGCTCATGTTCGGAGGaa                                                                          <  1:538120/242‑1 (MQ=255)                                                                                                                                                   accaccAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTCACCACAACAGGGGATTTGAAATAATCTTCTGCCAAATAGCGCTCATGTTCGGAGGAAAgg                                                                       >  1:467811/1‑251 (MQ=255)                                                                                                                                                     caccaATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGaa                                                                                                                                                               >  1:44866/1‑161 (MQ=255)                                                                                                                                                      accaATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTCACCACAACAGGGGATTTGAAATAATCTTCTGCCAAATAGCGCTCATGTTCGGAGGAAAGGTCa                                                                    >  1:454650/1‑251 (MQ=255)                                                                                                                                                      accaATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTCACCACAACAGGGGATTTGAAATAATCTTCTGCCAAATAGCGCTCATGTTCGGAGGAAAGGTCa                                                                    >  1:377910/1‑251 (MQ=255)                                                                                                                                                       ccaATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTCACCACAACAGGGGATTTGAAATAATCTTCTGCCAAATAGCGCTCATGTTCGGAGGAAAGGTCaa                                                                   >  1:282093/1‑251 (MQ=255)                                                                                                                                                           taatCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATCCGTCTAAAC‑GTTCTTCCCGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTCACCACAACAGGGGATTTGAAATAATCTTCTGCCAAATAGCGCTCATGTTCGGAGGAAAGGTCAATGcc                                                               <  1:807123/251‑1 (MQ=255)                                                                                                                                                                  ccGGATTTAGCCCCATTCCCGCCATGCTTTTGTCTAATACGTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTCACCACAACAGGGGATTTGAAATAATCTTCTGCCAAATAGCGCTCATGTTCGGAGGAAAGGTCAATGCCCCAAGaa                                                        <  1:110777/251‑1 (MQ=255)                                                                                                                                                                     gATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAAC‑GTTCTTCAGGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTCACCACAACAGGGGATTTGAAATAATCTTCTGCCAAATAGCGCTCATGTTCGGAGGAAAGGTCAATGCCCCAAGAAAc                                                      <  1:154598/250‑1 (MQ=255)                                                                                                                                                                           gCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTCACCACAACAGGGGATTTGAAATAATCTTCTGCCAAATAGCGCTCATGTTCGGAGGAAAGGTCa                                                                    >  1:343846/1‑230 (MQ=255)                                                                                                                                                                              ccATTTCCGCCATGCGTTTGTCTAATACGTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTCACCACAACAg                                                                                                                         <  1:124692/174‑1 (MQ=255)                                                                                                                                                                                     cGCCATGCGTTTGTCTAATACGTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTCACCACAACAGGGGATTTGAAATAATCTTCTGCCAAATAGCGCTCATGTTCGGAGGAAAGGTCAATGCCCCAAGAAACCGGGAATTCAAAGGttt                                     >  1:829387/1‑251 (MQ=255)                                                                                                                                                                                         aTGCTTTTGTCTAATACGTCTAAGC‑TTTCTTCCCTTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTCACCACAACAGGGGATTTGAAATAATCTTCTGCCAAATAGCGCTCATGTTCGGATGAAAGGTCAATGCCCCAAGAAACCGGGAATTCAAAGGTTTTAcc                                 <  1:447847/251‑1 (MQ=255)                                                                                                                                                                                         aTGCGTTTGTCTAATACGTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTCACCACAACAGGGGATTTGAAATAATCTTCTGCCAAATAGCGCTCATGTTCGGAGGAAAGGTCAATGCCCCAAGAAACCGGGAATTCAAAGGTTTTAcc                                 <  1:272469/251‑1 (MQ=255)                                                                                                                                                                                           gCGTTTGTCTAATACGTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTCACCACAACAGGGGATTTGAAATAATCTTCTGCCAAATAGCGCTCATGTTCGGAGGAAAGGTCAATGCCCCAAGAAACCGGGAATTCAAAGGTTTTACCgg                               >  1:149162/1‑251 (MQ=255)                                                                                                                                                                                                 gTCTAATACGTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTCACCACAACAgggg                                                                                                                      >  1:6257/1‑158 (MQ=255)                                                                                                                                                                                                 gTCTAATACGTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTCACCACAACAGGGGATTTGAAATAATCTTCTGCCAAATAGCGCTCATGTTCGGAGGAAAGGTCAATGCCCCAAGAAACCGGGAATTCAAAGGTTTTACCGGATTTCa                         >  1:220519/1‑251 (MQ=255)                                                                                                                                                                                                 gTCTAATACGTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTCACCACAACAGGGGATTTGAAATAATCTTCTGCCAAATAGCGCTCATGTTCGGAGGAAAGGTCAATGCCCCAAGAAACCGGGAATTCAAAGGTTTTACCGGATTTCa                         >  1:110582/1‑251 (MQ=255)                                                                                                                                                                                                 gTCTAATACGTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTCACCACAACAGGGGATTTGAAATAATCTTCTGCCAAATAGCGCTCATGTTCGGAGGAAAGGTCAATGCCCCAAGAAACCGGGAATTCAAAGGTTTTACCGGATTTCa                         >  1:487137/1‑251 (MQ=255)                                                                                                                                                                                                   cTAATACGTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTCACCACAACAGGGGATTTGAAATAATCTTCTGCCAAATAGCGCTCATGTTCGGAGGAAAGGTCAATGCCCCAAGAAACCGGGAATTCAAAGGTTTTACCGGATTTCAGt                       <  1:616189/251‑1 (MQ=255)                                                                                                                                                                                                         cGTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTCACCACAACAGGGGATTTGAAATAATCTTCTGCCAAATAGCGCTCATGTTCGGAGGAAAGGTCAATGCCCCAAGAAACCGGGAATTCAAAGGTTTTACCGGATTTCAGTAAAAt                  >  1:423466/1‑250 (MQ=255)                                                                                                                                                                                                         cGTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTCACCACAACAGGGGATTTGAAATAATCTTCTGCCAAATAGCGCTCATGTTCGGAGGAAAGGTCAATGCCCCAAGAAACCGGGAATTCAAAGGTTTTACCGGATTTCAGTAAAAt                  >  1:182051/1‑250 (MQ=255)                                                                                                                                                                                                         cGTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTCACCACAACAGGGGATTTGAAATAATCTTCTGCCAAATAGCGCTCATGTTCGGAGGAAAGGTCAATGCCCCAAGAAACCGGGAATTCAAAGGTTTTACCGGATTTCAGTAAAAt                  >  1:467169/1‑250 (MQ=255)                                                                                                                                                                                                          gTCTAAAC‑GTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTCACCACAACAGGGGATTTGAAATAATCTTCTGCCAAATAGCGCTCATGTTCGGAGGAAAGGTCAATGCCCCAAGAAACCGGGAATTCAAAGGt                                       >  1:119702/1‑228 (MQ=255)                                                                                                                                                                                                                 c‑GTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTCACCACAACAGGGGATTTGAAATAATCTTCTGCCAAATAGCGCTCATGTTCGGAGGAAAGGTCAATGCCCCAAGAAACCGGGAATTCAAAGGTTTTACCGGATTTCAGTAAAATCTCAATGGc         <  1:400219/251‑1 (MQ=255)                                                                                                                                                                                                                         cACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTCACCACAACAGGGGATTTGAAATAATCTTCTGCCAAATAGCGCTCATGTTCGGAGGAAAGGTCAATGCCCCAAGAAACCGGGAATTCAAAGGTTTTACCGGATTTCAGTAAAATCTCAATGGCGTCGgtg  <  1:387193/251‑1 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 GAATTAGAAATTTGCGTTACGCGGAGCACGTGGGAACGGGATCACATCACGAATATTTTGCAACCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACGACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAAC‑GTTCTTCACGTTGCGAGCCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTCACCACAACAGGGGATTTGAAATAATCTTCTGCCAAATAGCGCTCATGTTCGGAGGAAAGGTCAATGCCCCAAGAAACCGGGAATTCAAAGGTTTTACCGGATTTCAGTAAAATCTCAATGGCGTCGGTG  >  1/1184230‑1184695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑