Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,184,361	G→A	intergenic (+24781/+236576)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,184,361	0	G	A	100.0%	177.2 / NA	68	intergenic (+24781/+236576)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (44/24);  total (44/24)

TTGGTATCTTTTTTGTTATCGGGGAAACAAAAGTGTGGTCGTTTTTTCCGGCAGTTTTGAAAGTATAAAAAAATCGCCTTGCTATATAAACAAGGCGATTTTTACGTTTATGATGAATTAGAAATTTGCGTTACGCGGAGCACGTGGGAACGGGATCACATCACGAATATTTTGCAACCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACGACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAACGTTCTTCACGTTGCGAGCCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTCACCACAACAGGGGATTTGAAATAATCTTCTGCCAAATAG  >  1/1184116‑1184605                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     ttGGTATCTTTTTTGTTATCGGGGAAACAAAAGTGTGGTCGTTTTTTCCGGCAGTTTTGAAAGTATAAAAAAATCGCCTTGCTATATAAACAAGGCGATTTTTACGTTTATGATGAATTAGAAATTTGCGTTACGCGGAGCACGTGGGAACGGGATCACATCACGAATATTTTGCAACCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCa                                                                                                                                                                                                                                                 >  1:451809/1‑251 (MQ=255)                        gAAACAAAAGTGTGGTCGTTTTTTCCGGCAGTTTTGAAAGTATAAAAAAATCGCCTTGCTATATAAACAAGGCGATTTTTACGTTTATGATGAATTAGAAATTTGCGTTACGCGGAGCACGTGGGAACGGGATCACATCACGAATATTTTGCAACCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATc                                                                                                                                                                                                                           <  1:630794/250‑1 (MQ=255)                         aaaCAAAAGTGTGGTCGTTTTTTCCGGCAGTTTTGAAAGTATAAAAAAATCGCCTTGCTATATAAACAAGGCGATTTTTACGTTTATGATGAATTAGAAATTTGCGTTACGCGGAGCACGTGGGAACGGGATCACATCACGAATATTTTGCAACCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCtt                                                                                                                                                                                                                         >  1:714412/1‑251 (MQ=255)                         aaaCAAAAGTGTGGTCGTTTTTTCCGGCAGTTTTGAAAGTATAAAAAAATCGCCTTGCTATATAAACAAGGCGATTTTTACGTTTATGATGAATTAGAAATTTGCGTTACGCGGAGCACGTGGGAACGGGATCACATCACGAATATTTTGCAACCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCt                                                                                                                                                                                                                          <  1:378377/250‑1 (MQ=255)                          aaCAAAAGTGTGGTCGTTTTTTCCGGCAGTTTTGAAAGTATAAAAAAATCGCCTTGCTATATAAACAAGGCGATTTTTACGTTTATGATGAATTAGAAATTTGCGTTACGCGGAGCACGTGGGAACGGGATCACATCACGAATATTTTGCAACCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCtt                                                                                                                                                                                                                         >  1:490153/1‑250 (MQ=255)                            cAAAAGTGTGGTCGTTTTTTCCGGCAGTTTTGAAAGTATAAAAAAATCGCCTTGCTATATAAACAAGGCGATTTTTACGTTTATGATGAATTAGAAATTTGCGTTACGCGGAGCACGTGGGAACGGGATCACATCACGAATATTTTGCAACCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCaataa                                                                                                                                                                                                                             >  1:366691/1‑244 (MQ=255)                            cAAAAGTGTGGTCGTTTTTTCCGGCAGTTTTGAAAGTATAAAAAAATCGCCTTGCTATATAAACAAGGCGATTTTTACGTTTATGATGAATTAGAAATTTGCGTTACGCGGAGCACGTGGGAACGGGATCACATCACGAATATTTTGCAACCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCg                                                                                                                                                                                                                      >  1:617006/1‑251 (MQ=255)                                          ttttttCCGGCAGTTTTGAAAGTATAAAAAAATCGCCTTGCTATATAAACAAGGCGATTTTTACGTTTATGATGAATTAGAAATTTGCGTTACGCGGAGCACGTGGGAACGGGATCACATCACGAATATTTTGCAACCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCAtt                                                                                                                                                                                                        <  1:398516/251‑1 (MQ=255)                                                      gTTTTGAAAGTATAAAAAAATCGCCTTGCTATATAAACAAGGCGATTTTTACGTTTATGATGAATTAGAAATTTGCGTTACGCGGAGCACGTGGGAACGGGATCACATCACGAATATTTTGCAACCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCAGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGt                                                                                                                                                                                            >  1:433351/1‑251 (MQ=255)                                                               gTATAAAAAAATCGCCTTGCTATATAAACAAGGCGATTTTTACGTTTATGATGAATTAGAAATTTGCGTTACGCGGAGCACGTGGGAACGGGATCACATCACGAATATTTTGCAACCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGAttt                                                                                                                                                                                                                 >  1:139969/1‑221 (MQ=255)                                                                tataAAAAAATCGCCTTGCTATATAAACAAGGCGATTTTTACGTTTATGATGAATTAGAAATTTGCGTTACGCGGAGCACGTGGGAACGGGATCACATCACGAATATTTTGCAACCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATa                                                                                                                                                                                  <  1:215437/251‑1 (MQ=255)                                                                                 gCTATATAAACAAGGCGATTTTTACGTTTATGATGAATTAGAAATTTGCGTTACGCGGAGCACGTGGGAACGGGATCACATCACGAATATTTTGCAACCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTc                                                                                                                                                                              <  1:537365/238‑1 (MQ=255)                                                                                 gCTATATAAACAAGGCGATTTTTACGTTTATGATGAATTAGAAATTTGCGTTACGCGGAGCACGTGGGAACGGGATCACATCACGAATATTTTGCAACCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAACGTTCTTCa                                                                                                                                                                 >  1:324202/1‑251 (MQ=255)                                                                                 gCTATATAAACAAGGCGATTTTTACGTTTATGATGAATTAGAAATTTGCGTTACGCGGAGCACGTGGGAACGGGATCACATCACGAATATTTTGCAACCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAACGTTCTTCa                                                                                                                                                                 >  1:363208/1‑251 (MQ=255)                                                                                 gCTATATAAACAAGGCGATTTTTACGTTTATGATGAATTAGAAATTTGCGTTACGCGGAGCACGTGGGAACGGGATCACATCACGAATATTTTGCAACCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAACGTTCTTCa                                                                                                                                                                 >  1:180269/1‑251 (MQ=255)                                                                                  cTATATAAACAAGGCGATTTTTACGTTTATGATGAATTAGAAATTTGCGTTACGCGGAGCACGTGGGAACGGGATCACATCACGAATATTTTGCAACCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATc                                                                                                                                                                                                                                             >  1:324153/1‑174 (MQ=255)                                                                                  cTATATAAACAAGGCGATTTTTACGTTTATGATGAATTAGAAATTTGCGTTACGCGGAGCACGTGGGAACGGGATCACATCACGAATATTTTGCAACCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGc                                                                                                                                                                                                   >  1:70523/1‑216 (MQ=255)                                                                                  cTATATAAACAAGGCGATTTTTACGTTTATGATGAATCAGAAATTTGCGTTACGCGGAGCACGTGGGAACGGGATCACATCACGAATATTTTGCAACCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAACGTTCTTCAc                                                                                                                                                                <  1:839427/251‑1 (MQ=255)                                                                                    atataAACAAGGCGATTTTTACGTTTATGATGAATTAGAAATTTGCGTTACGCGGAGCACGTGGGAACGGGATCACATCACGAATATTTTGCAACCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAACGTTCTTCACGt                                                                                                                                                              >  1:853330/1‑251 (MQ=255)                                                                                           cAAGGCGATTTTTACGTTTATGATGAATTAGAAATTTGCGTTACGCGGAGCACGTGGGAACGGGATCACATCACGAATATTTTGCAACCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCC‑AAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAACGttcttc                                                                                                                                                                  <  1:616987/239‑1 (MQ=255)                                                                                                           tttATGATGAATTAGAAATTTGCGTTACGCGGAGCACGTGGGAACGGGATCACATCACGAATATTTTGCAACCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTc                                                                                                                                                                              <  1:434448/212‑1 (MQ=255)                                                                                                                gatgaATTAGAAATTTGCGTTACGCGGAGCACGTGGGAACGGGATCACATCACGAATATTTTGCAACCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCa                                                                                                                                                                                                                                            >  1:88825/1‑145 (MQ=255)                                                                                                                   gaATTAGAAATTTGCGTTACGCGGAGCACGTGGGAACGGGATCACATCACGAATATTTTGCAACCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCt                                                                                                                                                                                      >  1:314200/1‑196 (MQ=255)                                                                                                                   gaATTAGAAATTTGCGTTACGCGGAGCACGTGGGAACGGGATCACATCACGAATATTTTGCAACCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAACGTTCTTCACGTTGCGAACCGCCGATAAt                                                                                                                                             >  1:581780/1‑237 (MQ=255)                                                                                                                    aaTTAGAAATTTGCGTTACGCGGAGCACGTGGGAACGGGATCACATCACGAATATTTTGCAACCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGAt                                                                                                                                                                                                                   >  1:34428/1‑166 (MQ=255)                                                                                                                    aaTTAGAAATTTGCGTTACGCGGAGCACGTGGGAACGGGATCACATCACGAATATTTTGCAACCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATAc                                                                                                                                                                                 >  1:285477/1‑200 (MQ=255)                                                                                                                          aaaTTTGCGTTACGCGGAGCACGTGGGAACGGGATCACATCACGAATATTTTGCAACCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAACGTTCTTCACGTTGCGAACCGCCGATAATTTCAcc                                                                                                                                       >  1:806297/1‑236 (MQ=255)                                                                                                                                gCGTTACGCGGAGCACGTGGGAACGGGATCACATCACGAATATTTTGCAACCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTc                                                                                                                                                                                       >  1:29436/1‑182 (MQ=255)                                                                                                                                gCGTTACGCGGAGCACGTGGGAACGGGATCACATCACGAATATTTTGCAACCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAAc                                                                                                                                                                         <  1:410078/196‑1 (MQ=255)                                                                                                                                     aCGCGGAGCACGTGGGAACGGGATCACATCACGAATATTTTGCAACCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATc                                                                                                                                                                                                                           >  1:895575/1‑141 (MQ=255)                                                                                                                                          gAGCACGTGGGAACGGGATCACATCACGAATATTTTGCACCCCTGTCACATAAACAATTAAACGTTCAAACCCCAAGCCAAAGCCGGAAGGCGGACCCGTGCCGTAACACCGCAAATCACGATACCACCAATAATCTCCCGGATTTAGCCCCATTCCCGCCATGCGTTTGTCTAATACGTCTAAACGTTCTTCACGTTGCGAACCGCCGATAATTTCAcc                                                                                                                                       <  1:390489/220‑1 (MQ=255)                                                                                                                                          gAGCACGTGGGAACGGGATCACATCACGAATATTTTGCAACCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAACGTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCa                                                                                                        >  1:157029/1‑251 (MQ=255)                                                                                                                                               cGTGGGAACGGGATCACATCACGAATATTTTGCAACCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAACGTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGt                                                                                                   >  1:197478/1‑251 (MQ=255)                                                                                                                                                  gggAACGGGATCACATCACGAATATTTTGCAACCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCt                                                                                                                                                                                      >  1:684309/1‑165 (MQ=255)                                                                                                                                                     aaCGGGTTCACATCCCGAATATTTTGCACCCCTGTCACAAAAACAATTAAACTTAAAAACCCCAAGCCAAAGCCGAAATGCGGACCCGTGCCATAACAACGAAAATCACGATCCCCCCAATAATCTTCCGGATTTGGCCCCATTCCCGCCATGCTTTTGTCTAATCCGTCTAAACGTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTcc                                                                                             <  1:320376/251‑1 (MQ=255)                                                                                                                                                         ggATCACATCACGAATATTTTGCACCCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGACCCGTGCCGTAACAACGCAAATCACGATCCCACCAATAATCTCCCGGATTTAGCCCCATTCCCGCCATGCGTTTGTCTAATCCGTCTAAACGTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTc                                                                                              <  1:15484/246‑1 (MQ=255)                                                                                                                                                         ggATCACATCACGAATATTTTGCAACCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAACGTTCTTCACGTTGCGAACCGCCGATAATTTCAc                                                                                                                                        >  1:142697/1‑204 (MQ=255)                                                                                                                                                         ggATCACATCACGAATATTTTGCAACCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAACGTTCTTCACGTTGCGAACCGCCGATAATTTCACCaa                                                                                                                                     >  1:11730/1‑207 (MQ=255)                                                                                                                                                                  cacGAATTTTTTGAAACCCTGTCACATAAACAATTAACCGTTAAAAGCCCAAGCCAAAGCCGGAATGCGGACCCGTGCCGTAACAACGCAAACCACGATCCCCCCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAACGTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTc                                                                                              <  1:387449/237‑1 (MQ=255)                                                                                                                                                                  cacGAATATTTTGCAACCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAACGTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAg                                                                                >  1:355217/1‑251 (MQ=255)                                                                                                                                                                  cacGAATATTTTGCAACCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAACGTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAg                                                                                >  1:476432/1‑251 (MQ=255)                                                                                                                                                                             tGCAACCCTGTCACAAAAAAAATTAAACGTTAAAAGCCCAAGCAAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATCCGTCTAAACGTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGaa                                                                     <  1:91117/251‑1 (MQ=255)                                                                                                                                                                                 aCCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCt                                                                                                                                                                                      >  1:566393/1‑134 (MQ=255)                                                                                                                                                                                 aCCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAACGTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAgg                                                                   >  1:818436/1‑249 (MQ=255)                                                                                                                                                                                    cTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATAc                                                                                                                                                                                 <  1:279121/136‑1 (MQ=255)                                                                                                                                                                                      gTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAACGTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAAt                                                            >  1:545296/1‑251 (MQ=255)                                                                                                                                                                                       tCACAAAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGACCCGGGCCGTAACACCGCAAATCACGATCCTCCCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATCCGTCTAAACGTTCTTCACGTGGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAAt                                                            <  1:426404/250‑1 (MQ=255)                                                                                                                                                                                        cacaTAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCa                                                                                                                                                                                                                                            <  1:471687/73‑1 (MQ=255)                                                                                                                                                                                        cacaTAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAACGTTCTTCAc                                                                                                                                                                >  1:634762/1‑149 (MQ=255)                                                                                                                                                                                        cacaTAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAACGTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAAttt                                                          >  1:397338/1‑251 (MQ=255)                                                                                                                                                                                        cacaTAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAACGTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAAttt                                                          >  1:195012/1‑251 (MQ=255)                                                                                                                                                                                                cAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGACCCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATCCGTCTAAACGTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGaa                                                                     <  1:872604/232‑1 (MQ=255)                                                                                                                                                                                                cAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAACGTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGa                                                  >  1:670036/1‑251 (MQ=255)                                                                                                                                                                                                cAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAACGTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGa                                                  >  1:346762/1‑251 (MQ=255)                                                                                                                                                                                                         gTTCAAAGCCAAAGCAAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACAAAAAATCTCCGGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAACGTTCTTCGCGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGtttt                                           <  1:716542/249‑1 (MQ=255)                                                                                                                                                                                                          ttCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAACGTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAg                                                                       >  1:639203/1‑220 (MQ=255)                                                                                                                                                                                                            aaaaGCCCAAGCCAAAGCCGGAATGCGGACCCGTGCCGTAACAACGCAAATCACGATACCCCCAATAATCTTCCGGATTTAGCCCCATTCCCGCCATGCGTTTGTCTAATACGTCTAACCGTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTcacc                                      <  1:418037/250‑1 (MQ=255)                                                                                                                                                                                                                 cccAAGCAAAAGCCGGAATGCGGAACCGTGCCGTACCAACGCAAACCACGATCCCACAAATAATCTCCCGGATTTAGCCCCATTCCCGCCATGCGTTTGTCTAATACGTCTAAACGTTCTTCCCGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTCACCacaac                                 <  1:745855/251‑1 (MQ=255)                                                                                                                                                                                                                      gCCAAAGCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAACGTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTCACCACAACAgggg                            >  1:150810/1‑251 (MQ=255)                                                                                                                                                                                                                            gCCGGAATGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAACGTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTCACCACAACAGGGGATTTGa                      >  1:229881/1‑251 (MQ=255)                                                                                                                                                                                                                              cGGAATGCGGACCCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAACGTTCTTCACTTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTCACCACAACAGGGGATTTGaaa                    <  1:719648/251‑1 (MQ=255)                                                                                                                                                                                                                                  aTGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAACGTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAAt                                                            >  1:648823/1‑207 (MQ=255)                                                                                                                                                                                                                                  aTGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAACGTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTcacc                                      >  1:37358/1‑229 (MQ=255)                                                                                                                                                                                                                                  aTGCGGAACCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAACGTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTcac                                       >  1:646891/1‑228 (MQ=255)                                                                                                                                                                                                                                   tGCGGACCCGTGCCGTAACACCGCAAATCCCGATACCACCAATAATCTTCCGGATTTAGCCCCATTCCCGCCATGCGTTTGTCTAATCCGTCTAAACGTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTCACCACAACAGGGGATTTGAAATAAtc               <  1:209503/251‑1 (MQ=255)                                                                                                                                                                                                                                     cGGAGCCGGGCCGAAACAACGCAAATCACGATCCCACAAATAATCTTCCGAATTTAGCCCCATTGCCGCCATGCGTTTGTCAAATCCGTCAAAACTTTCTTCACGTGGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTCACCACAACAGGGGATTTGAAATAAtct              <  1:394106/250‑1 (MQ=255)                                                                                                                                                                                                                                         aCCGTGCCGTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAACGTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTCACCACAACAGGGGATTTGAAATAATCTTCTg          >  1:175573/1‑250 (MQ=255)                                                                                                                                                                                                                                                 gTAACAACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAACGTTCTTCACGTTGCGAACCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTCACCACAACAGGGGATTTGAAATAATCTTCTGCCAAATAg  >  1:561997/1‑250 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                     TTGGTATCTTTTTTGTTATCGGGGAAACAAAAGTGTGGTCGTTTTTTCCGGCAGTTTTGAAAGTATAAAAAAATCGCCTTGCTATATAAACAAGGCGATTTTTACGTTTATGATGAATTAGAAATTTGCGTTACGCGGAGCACGTGGGAACGGGATCACATCACGAATATTTTGCAACCCTGTCACATAAACAATTAAACGTTCAAAGCCCAAGCCAAAGCCGGAATGCGGAACCGTGCCGTAACGACGCAAATCACGATACCACCAATAATCTTCCGGATTTAGCCCCATTTCCGCCATGCGTTTGTCTAATACGTCTAAACGTTCTTCACGTTGCGAGCCGCCGATAATTTCACCAATGCCCGGCGCTAAAACATCCATTGCCGCAACGGTTTGTCCATCGTCATTTAAGCGCATATAGAAGGCTTTAATTTCTTTCGGATAGTTTTTCACCACAACAGGGGATTTGAAATAATCTTCTGCCAAATAG  >  1/1184116‑1184605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑