Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,181,574	C→T	intergenic (+21994/+239363)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,181,574	0	C	T	89.2%	144.1 / ‑2.9	67	intergenic (+21994/+239363)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base C (2/0);  major base T (23/35);  minor base G (7/0);  total (32/35)
Fisher's exact test for biased strand distribution p-value = 2.92e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAAATTGCACCTTTGTTTATTCGGCGAGGAAGGCATTGAATTACGCGATTTGTTACAATACGATAAGCAGCAACTCATTTTGCAATCAAGAATTTTTTCTGCGTTACAAGGTAAGCGTGAACATCATTATCTGCACATCGGCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTAATGCA‑CGCC‑G‑A‑TTTTGAAAAACAGCAAG‑AAAATCAACCGCACTTTAATTATCATGG‑CCACATTTACTCA‑CA‑TT‑AATTCCCATGGGGAAGCCAATATGGTGGACGTTTCC‑GCCAAAGCCGAAACCGTCCGTGAAGCCCGCGCTGAGGCTTATGTTTGTATGGCGCCCGAAACCTTACAAATGATCCTGTCCGGTCAGCATCACAAAGGCGATGTGTTCGCCACCGCCCGCATTGCCGGCATTCAGGCGGCAAAACGCACGTGGGAATTGATTCCCTTGTGCCATCCGTTATTGCTT  >  1/1181335‑1181812                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   gAAATTGCACCTTTGTTTATTCGGCGAGGAAGGCATTGAATTACGCGATTTGTTACAATACGATAAGCAGCAACTCATTTTGCAATCAAGAATTTTTTCTGCGTTACAAGGTAAGCGTGAACATCATTATCTGCACATCGGCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTAATGCA‑CGCC‑G‑G‑TTTTGTAAAAAAGGAAA‑AAAAACAACCGCACCTTTATTATAATGG‑TCACAttttatt                                                                                                                                                                                                                                         >  1:94136/1‑247 (MQ=255)  aaaTTGCACCTTTGTTTATTCGGCGAGGAAGGCATTGAATTACGCGATTTGTTACAATACGATAAGCAGCAACTCATTTTGCAATCAAGAATTTTTTCTGCGTTACAAGGTAAGCGTGAACATCATTATCTGCACATCGGCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTAATGCA‑CGCC‑G‑A‑TTTTGAAAAACAGCAAG‑AAAATCAACCGCACTTTAATTATCATGG‑TCACATTTACTc                                                                                                                                                                                                                                         <  1:27230/250‑1 (MQ=255)  aaaTTGCACCTTTGTTTATTCGGCGAGGAAGGCATTGAATTACGCGATTTGTTACAATACGATAAGCAGCAACTCATTTTGCAATCAAGAATTTTTTCTGCGTTACAAGGTAAGCGTGAACATCATTATCTGCACATCGGCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTAATGCA‑CGCC‑G‑A‑TTTTGAAAAACAGCAAG‑AAAATCAACCGCACTTTAATTATCATGG‑TCACATTTACTc                                                                                                                                                                                                                                         <  1:749994/250‑1 (MQ=255)     ttGCACCTTTGTTTATTCGGCGAGGAAGGCATTGAATTACGCGATTTGTTACAATACGATAAGCAGCAACTCATTTTGCAATCAAGAATTTTTTCTGCGTTACAAGGTAAGCGTGAACATCATTATCTGCACATCGGCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTAATGCA‑CGCC‑G‑A‑TTTTGAAAAACAGCAAG‑AAAATCAACCGCACTTTAATTATCATGG‑TCACATTTACTCA‑CA‑t                                                                                                                                                                                                                                   <  1:511733/251‑1 (MQ=255)           cTTTGTTTATTCGGCGAGGAAGGCATTGAATTACGCGATTTGTTACAATACGATAAGCAGCAACTCATTTTGCAATCAAGAATTTTTTCTGCGTTACAAGGTAAGCGTGAACATCATTATCTGCACATCGGCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTAATGCA‑CGCC‑G‑A‑TTTTGAAAAACAGCAAG‑AAAATCAACCGCACTTTAATTATCATGG‑TCACATTTACTCA‑CA‑TT‑AATTc                                                                                                                                                                                                                            <  1:758091/251‑1 (MQ=255)            tttGTTTATTCGGCGAGGAAGGCATTGAATTACGCGATTTGTTACAATACGATAAGCAGCAACTCATTTTGCAATCAAGAATTTTTTCTGCGTTACAAGGTAAGCGTGAACATCATTATCTGCACATCGGCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTTATATC‑CGCCAC‑A‑TTTTTAAAAA‑AACAAG‑AAAAACAAACGCCCCTTTATTTTCATGG‑TCACCTTTACTCA‑CA‑AT‑AAttt                                                                                                                                                                                                                            >  1:258517/1‑249 (MQ=255)              tGTTTATTCGGCGAGGAAGGCATTGAATTACGCGATTTGTTACAATACGATAAGCAGCAACTCATTTTGCAATCAAGAATTTTTTCTGCGTTACAAGGTAAGCGTGAACATCATTATCTGCACATCGGCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTAATGTA‑CGCC‑G‑A‑TTTTGAAAAAAAAGAAA‑AAAAAAAAACGCACCTTAATTTTAATGG‑GCACATTTAATCA‑CA‑TA‑AAtttcc                                                                                                                                                                                                                          >  1:413606/1‑247 (MQ=255)                         cGAGGAAGGCATTGAATTACGCGATTTGTTACAATACGATAAGCAGCAACTCATTTTGCAATCAAGAATTTTTTCTGCGTTACAAGGTAAGCGTGAACATCATTATCTGCACATCGGCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTAATGCA‑CGCC‑G‑A‑TTTTGAAAAACAGCAAG‑AAAATCAACCGCACTTTAATTATCATGG‑TCACATTTACTCA‑CA‑TT‑AATTCCCATGGGGAAGcc                                                                                                                                                                                                               <  1:733175/250‑1 (MQ=255)                                 gCATTGAATTACGCGATTTGTTACAATACGATAAGCAGCAACTCATTTTGCAATCAAGAATTTTTTCTGCGTTACAAGGTAAGCGTGAACATCATTATCTGCACATCGGCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTAATGCA‑CGCC‑G‑A‑TTTTGAAAAACAGCAAG‑AAAATCAACCGCACTTTAATTATCATGG‑TCACATTTACTc                                                                                                                                                                                                                                         <  1:372700/219‑1 (MQ=255)                                     tGAATTACGCGATTTGTTACAATACGATAAGCAGCAACTCATTTTGCAATCAAGAATTTTTTCTGCGTTACAAGGTAAGCGTGAACATCATTATCTGCACATCGGCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTAATGCA‑CGCC‑G‑A‑TTTTGAAAAACAGCAAG‑AAAATCAACCGCACTTTAATTATCATGG‑TCACATTTACTCA‑CA‑TT‑AATTCCCATGGGGAAGCCAATATGGTGGACg                                                                                                                                                                                                  <  1:731511/251‑1 (MQ=255)                                      gAATTACGCGATTTGTTACAATACGATAAGCAGCAACTCATTTTGCAATCAAGAATTTTTTCTGCGTTACAAGGTAAGCGTGAACATCATTATCTGCACATCGGCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTAATGCA‑CGCC‑G‑A‑TTTTGAAAAACAGCAAG‑AAAATCAACCGCACTTTAATTATCATGG‑TCACATTTACTCA‑CA‑TT‑AATTCCCATGGGGAAGCCAATATGGTGGAc                                                                                                                                                                                                   <  1:387996/249‑1 (MQ=255)                                        aTTACGCGATTTGTTACAATACGATAAGCAGCAACTCATTTTGCAATCAAGAATTTTTTCTGCGTTACAAGGTAAGCGTGAACATCATTATCTGCACATCGGCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTAATGCA‑CGCC‑G‑A‑TTTTGAAAAACAGCAAG‑AAAATCAACCGCACTTTAATTATCATGG‑TCACATTTACTc                                                                                                                                                                                                                                         <  1:182124/212‑1 (MQ=255)                                                aTTTGTTACAATACGATAAGCAGCAACTCATTTTGCAATCAAGAATTTTTTCTGCGTTACAAGGTAAGCGTGAACATCATTATCTGCACATCGGCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTAATGCA‑CGCC‑G‑A‑TTTTTAAAAAAAACAAA‑AAAAACAAACGCACTTTTATTATCATGG‑TCACAATTAATca‑caa                                                                                                                                                                                                                                    >  1:362336/1‑207 (MQ=255)                                                aTTTGTTACAATACGATAAGCAGCAACTCATTTTGCAATCAAGAATTTTTTCTGCGTTACAAGGTAAGCGTGAACATCATTATCTGCACATCGGCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTAATGCA‑CGCC‑G‑A‑TTTTGAAAAACAGCAAG‑AAAATCAACCGCACTTTAATTATCATGG‑TCACATTTACTCA‑CA‑TT‑AATTCCCATgg                                                                                                                                                                                                                      <  1:55194/220‑1 (MQ=255)                                                aTTTGTTACAATACGATAAGCAGCAACTCATTTTGCAATCAAGAATTTTTTCTGCGTTACAAGGTAAGCGTGAACATCATTATCTGCACATCGGCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTAATGCA‑CGCC‑G‑A‑TTTTGAAAAACAGCAAG‑AAAATCAACCGCACTTTAATTATCATGG‑TCACATTTACTCA‑CA‑TT‑AATTCCCATGGGGAAGCCAATAtgg                                                                                                                                                                                                        <  1:299711/234‑1 (MQ=255)                                                aTTTGTTACAATACGATAAGCAGCAACTCATTTTGCAATCAAGAATTTTTTCTGCGTTACAAGGTAAGCGTGAACATCATTATCTGCACATCGGCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTAAAGTA‑CGCC‑C‑G‑TTTTTATAAACAACAAA‑AAAAACAAACCCACTTTTATTTTCATTG‑GCACATTTTCTCA‑CA‑TT‑AATTCCCCACGGGAAGCCAATAttgtgggaggttccccccaa                                                                                                                                                                                       >  1:399756/1‑237 (MQ=255)                                                  ttGTTACAATACGATAAGCAGCAACTCATTTTGCAATCAAGAATTTTTTCTGCGTTACAAGGTAAGCGTGAACATCATTATCTGCACATCGGCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGATAAAGAA‑CGCC‑G‑G‑TTTTTAAAAAAAGCAAA‑AAAATTAAACGCACTTTTATTATTTTGG‑CCACAATTACTCA‑CA‑AT‑AAATTCCAAGGGGAAGGCaaaataggggagggttttgccaaaa                                                                                                                                                                                      >  1:288468/1‑227 (MQ=255)                                                   tGTTACAATACGATAAGCAGCAACTCATTTTGCAATCAAGAATTTTTTCTGCGTTACAAGGTAAGCGTGAACATCATTATCTGCACATCGGCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTAATGCA‑CGCC‑C‑A‑CTTTTAAAAAAAACAAG‑AAAAACAACCGCACTTTAATTATCATGG‑GCACATTTACTTA‑CATTT‑ATTTCCCTTGGGG‑AGGCAAAAtggtgggaggttccgcccaaac                                                                                                                                                                                     >  1:850667/1‑234 (MQ=255)                                                    gTTACAATACGATAAGCAGCAACTCATTTTGCAATCAAGAATTTTTTCTGCGTTACAAGGTAAGCGTGAACATCATTATCTGCACATCGGCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTAATTCA‑CGCC‑G‑ATTTTTGAAAAA‑AGCAAA‑AAAAAAAAACCCACCTTAAATATAATGG‑TCAAAATTAATCA‑CA‑TTAAATTCCC‑TGGGGAAGGCAAAAAGGTGGAAGGTTCC‑GCCaaaaacg                                                                                                                                                                                  >  1:466331/1‑247 (MQ=255)                                                    gTTACAATACGATAAGCAGCAACTCATTTTGCAATCAAGAATTTTTTCTGCGTTACAAGGTAAGCGTGAACATCATTATCTGCACATCGGCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTAATGCA‑CGCC‑G‑A‑TTTTGAAAAACAGCAAG‑AAAATCAACCGCACTTTAATTATCATGG‑TCACATTTACTCA‑CA‑TT‑AATTCCCATGGGGAAGCCAatat                                                                                                                                                                                                          <  1:527808/228‑1 (MQ=255)                                                     ttACAATACGATAAGCAGCAACTCATTTTGCAATCAAGAATTTTTTCTGCGTTACAAGGTAAGCGTGAACATCATTATCTGCACATCGGCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTTATGCA‑CCCC‑C‑G‑TTTTTTAAAACAAGAAA‑AAAATCAACCCCACTTTCATTATCATGGTCCCCA‑TTAATCA‑CA‑TT‑AAATCCCCTGGGGGAACCAATATGGTGGACGGTTCC‑CCCCAAGCCGa                                                                                                                                                                                 >  1:261139/1‑251 (MQ=255)                                                     ttACAATACGATAAGCAGCAACTCATTTTGCAATCAAGAATTTTTTCTGCGTTACAAGGTAAGCGTGAACATCATTATCTGCACATCGGCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTAATGCA‑CGCC‑G‑A‑TTTTGAAAAACAGCAAG‑AAAATCAACCGCACTTTAATTATCATGG‑TCACATTTACTCA‑CA‑TT‑AATTCCCATGGGGAAGCCAATAtggtgg                                                                                                                                                                                                     <  1:165674/232‑1 (MQ=255)                                                       aCAATACGATAAGCAGCAACTCATTTTGCAATCAAGAATTTTTTCTGCGTTACAAGGTAAGCGTGAACATCATTATCTGCACATCGGCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTAATGCA‑CGCC‑G‑A‑TTTTTAAAAAAAACAAG‑AAAAACAAACGCACTTTTATTATTATGG‑TCACATTTACTCA‑CA‑TT‑AATTCCCATGGGGGAGGCAATAtgg                                                                                                                                                                                                        >  1:201005/1‑227 (MQ=255)                                                            aCGATAAGCAGCAACTCATTTTGCAATCAAGAATTTTTTCTGCGTTACAAGGTAAGCGTGAACATCATTATCTGCACTTCGGCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTAATGCA‑CGCC‑G‑A‑TTTTTAAAAAAAACAAG‑AAAAACAAACGCACTTTTATTATCATTG‑TCACATTTTCTCA‑CA‑TT‑AATTTCCATGGGGGAGCCAATATGGTGGGCGGTTCC‑GCCAAAACCGAAAccgt                                                                                                                                                                           >  1:15591/1‑250 (MQ=255)                                                            aCGATAAGCAGCAACTCATTTTGCAATCAAGAATTTTTTCTGCGTTACAAGGTAAGCGTGAACATCATTATCTGCACATCGGCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTTATTCA‑ACCC‑GCA‑TTTTTAAAAAAACCAAA‑AAAAAAAACC‑CCCTTTAATTATCCTTG‑GCACATTTACTCACCA‑TA‑AATCCCCTTGGGG‑AGCCCATATGGTGGAAGGTTCCTCCCAAACCCGAAAccg                                                                                                                                                                            >  1:363804/1‑250 (MQ=255)                                                            aCGATAAGCAGCAACTCATTTTGCAATCAAGAATTTTTTCTGCGTTACAAGGTAAGCGTGAACATCATTATCTGCACATCGGCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTTATGCA‑CGCC‑G‑A‑TTTTGAAAAACAACAAG‑AAAATCAACCGCACTTTAATTATCATGG‑TCACATTTACTCA‑CA‑TT‑AATTTCCATGGGGAAGCCAATATGGTGGGCGTTTTC‑GCCAAAACCGAAAacgt                                                                                                                                                                           >  1:588873/1‑250 (MQ=255)                                                            aCGATAAGCAGCAACTCATTTTGCAATCAAGAATTTTTTCTGCGTTACAAGGTAAGCGTGAACATCATTATCTGCACATCGGCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTAATTCA‑CGCC‑G‑A‑TTTTTAAAAAAAGCAAA‑AAAAACAACCGCACTTTTATTATCATTG‑GCACATTTACTCA‑CA‑TT‑AATTTCCCTGGGGGAGCCAATATTGTGGACGTTTCC‑GCCAAAACCGaaaacct                                                                                                                                                                           >  1:114468/1‑246 (MQ=255)                                                                            cATTTTGCAATCAAGAATTTTTTCTGCGTTACAAGGTAAGCGTGAACATCATTATCTGCACATCGGCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTAATGCA‑CGCC‑G‑A‑TTTTGAAAAACAGCAAG‑AAAATCAACCGCACTTTAATTATCATGG‑TCACATTTACTCA‑CA‑TT‑AATTCCCATGGGGAAGCCAATATGGTGGACGTTTCC‑GCCAAAGCCGAAACCGTCCGTGAAGCCCGCGCTg                                                                                                                                                          <  1:400091/251‑1 (MQ=255)                                                                              ttttGCAATCAAGAATTTTTTCTGCGTTACAAGGTAAGCGTGAACATCATTATCTGCACATCGGCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTAATGCA‑CGCC‑G‑A‑TTTTGAAAAACAGCAAG‑AAAATCAACCGCACTTTAATTATCATGG‑TCACATTTACTCA‑CA‑TT‑AATTc                                                                                                                                                                                                                            <  1:429311/184‑1 (MQ=255)                                                                                      tCAAGAATTTTTTCTGCGTTACAAGGTAAGCGTGAACATCATTATCTGCACATCGGCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTAATGCA‑CGCC‑G‑A‑TTTTGAAAAACAGCAAG‑AAAATCAACCGCACTTTAATTATCATGG‑TCACATTTACTCA‑CA‑TT‑AATTCCCATGGGGAAGCCAATATGGTGGACGTTTCC‑GCCAAAGCCGAAACCGTCCGTGAAGCCCGCGCTGAGGCTTATGt                                                                                                                                                <  1:375268/251‑1 (MQ=255)                                                                                       cAAGAATTTTTTCTGCGTTACAAGGTAAGCGTGAACATCATTATCTGCACATCGGCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTAATGCA‑CGCC‑G‑A‑TTTTGAAAAACAGCAAG‑AAAATCAACCGCACTTTAATTATCATGG‑TCACATTTACTCA‑CA‑TT‑AATTCCCATGGGGAAGCCAATATGGTGGACGTTTCC‑GCCAAAGCCGAAACCGTCCGTGAAGCCCGCGCTGAGGCTTATGtt                                                                                                                                               <  1:312210/251‑1 (MQ=255)                                                                                            aagaaacaggCGTTACAAGGTAAGCGTGAACATCATTATCTGCACATCGGCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTAATGCA‑CGCC‑G‑A‑TTTTGAAAAACAGCAAG‑AAAATCAACCGCACTTTAATTATCATGG‑TCACATTTACTCA‑CA‑TT‑AATTCCCATGGGGAAGCCAATATGGTGGACGTTTCC‑GCCAAAGCCGAAACCGTCCGTGAAGCCCGCGCTGAGGCTTATGTTTGTAt                                                                                                                                          <  1:772694/242‑1 (MQ=255)                                                                                            aTTTTTTCTGCGTTACAAGGTAAGCGTGAACATCATTATCTGCACATCGGCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTAATGCA‑CGCC‑G‑A‑TTTTGAAAAACAGCAAG‑AAAATCAACCGCACTTTAATTATCATGG‑TCACATTTACTCA‑CA‑TT‑AATTCCCATGGGGAAGCCAATATGGTGGACGTTTCC‑GCCAAAGCCGAAACCGTCCGTGAAGCCCGCGCTGAGGCTTATGTTTGTAt                                                                                                                                          <  1:430650/251‑1 (MQ=255)                                                                                                             aGGTAAGCGTGAACATCATTATCTGCACATCGGCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTAATGCA‑CGCC‑G‑A‑TTTTTAAAAAAAACAAG‑AAAAACAACCGCACTTTTATTATTATGG‑TCACATTTTCTCA‑CA‑TT‑AATTTCCCTGGGGGAGCCAATATTGTGGAGGTTTCC‑GCCAAAACCGAAACCGTCCGTGAAGCCCGCGCTGAGGCTTATGTTTGTATGGCGCCCGAAAcccta                                                                                                                          >  1:794017/1‑247 (MQ=255)                                                                                                             aGGTAAGCGTGAACATCATTATCTGCACATCGGCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTAATGCA‑CGCC‑G‑A‑TTTTGAAAAACAGCAAG‑AAAATCAACCGCACTTTAATTATCATGG‑TCACATTTACTCA‑CA‑TT‑AAtt                                                                                                                                                                                                                             <  1:615531/152‑1 (MQ=255)                                                                                                             aGGTAAGCGTGAACATCATTATCTGCACATCGGCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTAATGCA‑CGCC‑G‑A‑TTTTGAAAAACAGCAAG‑AAAATCAACCGCACTTTAATTATCATGG‑TCACATTTACTCA‑CA‑TT‑AATTCCCATGGGGAAGCCAATATGGTGGACGTTTCC‑GCCAAAGCCGAAACCGTCCGTGAAGCCCGCGCTGAGGc                                                                                                                                                      <  1:718695/222‑1 (MQ=255)                                                                                                             aGGTAAGCGTGAACATCATTATCTGCACATCGGCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTAATGCA‑CGCC‑G‑A‑TTTTGAAAAACAGCAAG‑AAAATCAACCGCACTGTAATTATCATGG‑TCACATTTACTCA‑CA‑TT‑AATTCCCATGGGGAAGCCAATATGGTGGACGTTTCC‑GCCAAAGCCGAAACCGTCCGTGAAGCCCGCGCTGAGGCTTATGt                                                                                                                                                <  1:58484/228‑1 (MQ=255)                                                                                                             aGGTAAGCGTGAACATCATTATCTGCACATCGGCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGCTTATACA‑CCCC‑C‑A‑TTTTTAAAAAAAGCCAGAAAAATAAACC‑CACTTTTATTATCATGG‑TCACAATTAATCACCA‑TA‑ATTTCCCTAGGGGAA‑CCAAAATCGTGGACGGTTCC‑GCCCAAACCCAAACCCTTCGCGTAGCCCGCGCTGAGGCTTTTGGTTTTATAGTGCCCCAAACCttt                                                                                                                          >  1:327155/1‑249 (MQ=255)                                                                                                                tAAGCGTGAACATCATTATCTGCACATCGGCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTAATGCA‑CGCC‑G‑A‑TTTTGAAAAACAGCAAG‑AAAATCAACCGCACTTTAATTATCATGG‑TCACATTTACTCA‑CA‑TT‑AATTCCCATGGGGAAGCCAATATGGTGGACGTTTCC‑GCCAAAGCCGAAACCGTCCGTGAAGCCCGCGCTGAGGCTTATGTTTGTATGGCGCCCGAAACCTTACaaa                                                                                                                      <  1:194584/251‑1 (MQ=255)                                                                                                                  aGCGTGAACATCATTATCTGCACATCGGCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTAATTCA‑CCCC‑G‑A‑TTTTTTAAAAAAACAAA‑AAAAACAAACCCACTTTTATTTTCATGG‑TTACATTTTCTCA‑CA‑TT‑AATTTCCATGGGGGAGCCAAAATGGTAGACGGTTCC‑CCCAAAACCGCAACCCTCCGTGAAGCCCCCGCCGAGGCTTATGTTTTTATGGGGCCCCAAACCCTACAAATg                                                                                                                    >  1:438616/1‑251 (MQ=255)                                                                                                                      tGAACATCATTATCTGCACATCGGCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTAATGCA‑CGCC‑G‑A‑TTTTGAAAAACAGCAAG‑AAAATCAACCGCACTTTAATTATCATGG‑TCACATTTACTCA‑CA‑TT‑AATTCCCATGGGGAAGCCAATATGGTGGACGTTTCC‑GCCAAAGCCGAAACCGTCCGTGAAGCCCGCGCTGAGGCTTATGTTTGTATGGCGCCCGAAACCTTACAAATGATcc                                                                                                                <  1:491158/251‑1 (MQ=255)                                                                                                                       gAACATCATTATCTGCACATCGGCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTAATTCA‑CGCC‑G‑ATTTTTGAAAAA‑AACAAA‑AAAAACAAACCCACCTTTATTATCATGG‑TCACATTTACTCA‑CA‑TT‑AATTTCCCTCGGGGAGGCAAAATGGTGGGCGTTTCC‑CCCAAAACCCAAAACCCCCCTGGAACCCGCGCCGCGGGTTTTGTTTTTATTGCGCCCCAAACCTTTCaaaagaacc                                                                                                                >  1:854290/1‑244 (MQ=255)                                                                                                                       gAACATCATTATCTGCACATCGGCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTAATGCA‑CGCC‑G‑G‑TTTTTAAAAAAAGCAAG‑AAAATCAAACGCACTTTAATTATTATGG‑GCACATTTACTCA‑CA‑TT‑AATTTCCATGGGGGAGCCAAAATGGTGGACGTTTCC‑GCCAAAACCGAAAACGTCCGTGAAGCCCCCGCCGAGGCTTTTGTTTTTATTGCGCCCGCAACCTTTCAAAAGATcc                                                                                                                >  1:68246/1‑250 (MQ=255)                                                                                                                         aCATCATTATCTGCACATCGGCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTAATGCA‑CGCC‑G‑A‑TTTTGAAAAACAGCAAG‑AAAATCAACCGCACTTTAATTATCATGG‑TCACATTTACTCA‑CA‑TT‑AATTCCCATGGGGAAGCCAATATGGTGGACGTTTCC‑GCCAAAGCCGAAACCGTCCGTGa                                                                                                                                                                     <  1:806019/195‑1 (MQ=255)                                                                                                                           atcatTATCTGCACATCGGCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTTATGCA‑CGCC‑G‑A‑TTTTTTAAAACAGCAAA‑AAAATCAACCGCACTTTTATTATCATGG‑GCACATTTACTCA‑CA‑TT‑AATTCCCCTGGGGGAGCCCATATGGTGGGCGTTTTC‑GCCAAAACCGAAACCCTCCCTGAAGCCCCCGCCGAGGCTTATTTTTTTATTGCGCCCGCAACCTTACAAAAAATCCTGTc                                                                                                            >  1:592206/1‑250 (MQ=255)                                                                                                                              atTATCTGCACATCGGCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTAATGCA‑CGCC‑G‑A‑TTTTGAAAAACAGCAAG‑AAAATCAACCGCACTTTAATTATCATGG‑TCACATTTACTCA‑CA‑TT‑AATTCCCATGGGGAAGCCAATATGGTGGACGTTTCC‑GCCAAAGCCGAAACCGTCCGTGAAGCCCGCGCTGAGGCTTATg                                                                                                                                                 <  1:97899/210‑1 (MQ=255)                                                                                                                                 aTCTGCACATCGGCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTTATTCACCCCC‑C‑A‑TTTTTAAAAA‑AGCAAGAAAAATCAACC‑CACCTTAATTCTCACTG‑TCACACTTATTCC‑CA‑AT‑AATTCCCCTCGGGGAGCCAATAtggtgggcggtttctcc                                                                                                                                                                                          >  1:39227/1‑156 (MQ=255)                                                                                                                                     gCACATCGGCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTAATGCA‑CGCC‑G‑A‑TTTTGAAAAACAGCAAG‑AAAATCAACCGCACTTTAATTATCATGG‑TCACATTTACTCA‑CA‑TT‑AATTCCCATGGGGAAGCCAATATGGTGGACGTTTCC‑GCCAAAGCCGAAACCGTCCGTGAAGCCCGCGCTGAGGCTTATg                                                                                                                                                 <  1:94239/203‑1 (MQ=255)                                                                                                                                            ggCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTAATGCA‑CGCC‑G‑A‑TTTTGAAAAACAGCAAG‑AAAATCAACCGCACTTTAATTATCATGG‑TCACATTTACTCA‑CA‑TT‑AATTc                                                                                                                                                                                                                            <  1:743942/122‑1 (MQ=255)                                                                                                                                              cGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTAATGCA‑CGCC‑G‑A‑TTTTGAAAAACAGCAAG‑AAAATCAACCGCACTTTAATTATCATGG‑TCACATTTACTCA‑CA‑TT‑AATTCCCATGGGGAAGCCAATATGGTGGACGTTTCC‑GCCAAAGCCGAAACCGTCCGTGAAGCCCGCGCTGAGGCTTATGTTTGTATGGCGCCCGAAACCTTACAAATGATCCTGTCCGGTCAGCATCACAAAGGc                                                                                         <  1:763609/250‑1 (MQ=255)                                                                                                                                                   gTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTAAAGCC‑CGCC‑G‑A‑TTTTTAAAAAAAGCAAG‑AAAAACAACCGCACCTTTATTATCATTG‑TCACAATTACTCA‑CA‑TT‑TATTCCCATGGGGGAGCCCATATGGTGGGCGTTTTC‑GCCCAAACCGAAACCGTCCCTGAAGCCCccgc                                                                                                                                                            >  1:873162/1‑178 (MQ=255)                                                                                                                                                         tacgtaGTCATTTGGCAAGTATCGGCGGTTAATGCA‑CGCC‑G‑A‑TTTTTAAAAAAAACAAA‑AAAAAAAAACGCACCTTTATTATTATGG‑TCACATTTAATCA‑CA‑AT‑AATTCCCATTGGGAAGCCAAAATGGTGGACGTTTCC‑GCCCAAACCGAAAACCTCCGTGAAGCCCGCGCTGAGGGTTATGTTTTTATGGCGCCCCAAAACTTACAAAAGATCCTGTCCGGTCAGCATCACAAAAGCGGAGTGTTCGc                                                                              >  1:487616/1‑250 (MQ=255)                                                                                                                                                             taGTCATTTGGCAAGTATCGGCGGTTAATGCA‑CCCC‑G‑A‑TTTTTAAAAAAAACAAG‑AAAAACAACCCCACTTTTATTATCATGG‑TCACATTTTCTCA‑CA‑TT‑AATTTCCATGGGGGAGCCAATATGGTGGGCGTTTCC‑GCCAAAACCGAAACCGTCCGTGAAGCCCGCGCCGAGGCTTTTGTTTGTTTGGCGCCCGAAACCTTACAAAAGATCCTGTCCGGGCAGCATCACAAAAGCGAAGTGGTTGCCAcc                                                                          >  1:281306/1‑250 (MQ=255)                                                                                                                                                                 cATTTGGCAAGTATCGGCGGTTAATGCA‑CGCC‑G‑A‑TTTTGAAAAACAGCAAG‑AAAATCAACCGCACTTTAATTATCATGG‑TCACATTTACTCA‑CA‑TT‑AATTCCCATGGGGAAGCCAATATGGTGGACGTTTCC‑GCCAAAGCCGAAACCGTCCGTGAAGCCCGCGCTGAGGCTTATGTTTGTATGGCGCCCGAAACCTTACAAATGATc                                                                                                                 <  1:887906/207‑1 (MQ=255)                                                                                                                                                                                   ggTTAATGCA‑CGCC‑G‑A‑TTTTGAAAAACAGCAAG‑AAAATCAACCGCACTTTAATTATCATGG‑TCACATTTACTCA‑CA‑TT‑AATTCCCATGGGGAAGCCAATATGGTGGACGTTTCC‑GCCAAAGCCGAAACCGTCCGTGAAGCCCGCGCTGAGGCTTATGTTTGTATGGCGCCCGAAACCTTACAAATGATCCTGTCCGGTCAGCATCACAAAGGCGATGTGTTCGCCACCGCCCGCATTGCCGGCATTCAgg                                                    >  1:376771/1‑250 (MQ=255)                                                                                                                                                                                    gTTAATGCA‑CGCC‑G‑A‑TTTTGAAAAACAGCAAG‑AAAATCAACCGCACTTTAATTATCATGG‑TCACATTTACTCA‑CA‑TT‑AATTCCCATGGGGAAGCCAATATGGTGGACGTTTCC‑GCCAAAGCCGAAACCGTCCGTGAAGCCCGCGCTGAGGCTTATGTTTGTATGGCGCCCGAAACCTTACAAATGATCCTGTCCGGTCAGCATCACAAAGGCGATGTGTTCGCCAccgcccgc                                                                    >  1:372039/1‑233 (MQ=255)                                                                                                                                                                                    gTTAATGCA‑CGCC‑G‑A‑TTTTGAAAAACAGCAAG‑AAAATCAACCGCACTTTAATTATCATGG‑TCACATTTACTCA‑CA‑TT‑AATTCCCATGGGGAAGCCAATATGGTGGACGTTTCC‑GCCAAAGCCGAAACCGTCCGTGAAGCCCGCGCTGAGGCTTATGTTTGTATGGCGCCCGAAACCTTACAAATGATCCTGTCCGGTCAGCATCACAAAGGCGATGTGTTCGCCACCGCCCGCATTGCCGGCATTCAggcg                                                  >  1:69813/1‑251 (MQ=255)                                                                                                                                                                                        aTGCA‑CGCC‑G‑A‑TTTTGAAAAACAGCAAG‑AAAATCAACCGCACTTTAATTATCATGG‑TCACATTTACTCA‑CA‑TT‑AATTCCCATGGGGAAGCCAATATGGTGGACGTTTCC‑GCCAAAGCCGAAACCGTCCGTGAAGCCCGCGCTGAGGCTTATGTTTGTATGGCGCCCGAAACCTTACAAATGATCCTGTCCGGTCAGCATCACAAAGGCGATGTGTTCGCCACCGCCCGCATTGCCGGCATTCAGGCGGCaa                                              >  1:557684/1‑251 (MQ=255)                                                                                                                                                                                                            aaaaaCAGCAAG‑AAAATCAACCGCACTTTAATTATCATGG‑TCACATTTACTCA‑CA‑TT‑AATTCCCATGGGGAAGCCAATATGGTGGACGTTTCC‑GCCAAAGCCGAAACCGTCCGTGAAGCCCGCGCTGAGGCTTATGTTTGTATGGCGCCCGAAACCTTACAAATGATCCTGTCCGGTCAGCATCACAAAGGCGATGTGTTCGCCACCGCCCGCATTGCCGGCATTCAGGCGGCAAAACGCACGTGGGAAt                               <  1:62263/250‑1 (MQ=255)                                                                                                                                                                                                             aaaaCAGCAAG‑AAAATCAACCGCACTTTAATTATCATGG‑TCACATTTACTCA‑CA‑TT‑AATTCCCATGGGGAAGCCAATATGGTGGACGTTTCC‑GCCAAAGCCGAAACCGTCCGTGAAGCCCGCGCTGAGGCTTATGTTTGTATGGCGCCCGAAACCTTACAAATGATCCTGTCCGGTCAGCATCACAAAGGCGATGTGTTCGCCACCGCCCGCATTGCCGGCATTCAGGCGGCAAAACGCACGTGGGAATTg                             <  1:750914/251‑1 (MQ=255)                                                                                                                                                                                                                          aaaTCAACCGCACTTTAATTATCATGG‑TCACATTTACTCA‑CA‑TT‑AATTCCCATGGGGAAGCCAATATGGTGGACGTTTCC‑GCCAAAGCCGAAACCGTCCGTGAAGCCCGCGCTGAGGCTTATGTTTGTATGGCGCCCGAAACCTTACAAATGATCCTGTCCGGTCAGCATCACAAAGGCGATGTGTTCGCCACCGCCCGCATTGCCGGCATTCAGGCGGCAAAACGCACGTGGGAATTGATTCCCTTGTGc                 <  1:24972/251‑1 (MQ=255)                                                                                                                                                                                                                           aaTCAACCGCACTTTAATTATCATGG‑TCACATTTACTCA‑CA‑TT‑AATTCCCATGGGGAAGCCAATATGGTGGACGTTTCC‑GCCAAAGCCGAAACCGTCCGTGAAGCCCGCGCTGAGGCTTATGTTTGTATGGCGCCCGAAACCTTACAAATGATCCTGTCCGGTCa                                                                                                      >  1:639285/1‑165 (MQ=255)                                                                                                                                                                                                                            aTCAACCGCACTTTAATTATCATGG‑TCACATTTACTCA‑CA‑TT‑AATTCCCATGGGGAAGCCAATATGGTGGACGTTTCC‑GCCAAAGCCGAAACCGTCCGTGAAGCCCGCGCTGAGGCTTATGTTTGTATGGCGCCCGAAACCTTACAAATGATCCTGTCCGGTCAGCATCACAAAGGCGATGTGTTCGCCACCGCCCGCATTGCCGGCATTCAGGCGGCAAAACGCACGTGGGAATTGATTCCCTTGTGCCa               <  1:105522/251‑1 (MQ=255)                                                                                                                                                                                                                                      cTTTAATTATCATGG‑TCACATTTACTCA‑CA‑TT‑AATTCCCATGGGGAAGCCAATATGGTGGACGTTTCC‑GCCAAAGCCGAAACCGTCCGTGAAGCCCGCGCTGAGGCTTATGTTTGTATGGCGCCCGAAACCTTACAAATGATCCTGTCCGGTCAGCATCACaactg                                                                                          <  1:257776/166‑4 (MQ=255)                                                                                                                                                                                                                                      cTTTAATTATCATGG‑TCACATTTACTCA‑CA‑TT‑AATTCCCATGGGGAAGCCAATATGGTGGACGTTTCC‑GCCAAAGCCGAAACCGTCCGTGAAGCCCGCGCTGAGGCTTATGTTTGTATGGCGCCCGAAACCTTACAAATGATCCTGTCCGGTCAGCATCACAAAGGCGATGTGTTCGCCACCGCCCGCATTGCCGGCATTCAGGCGGCAAAACGCACGTGGGAATTGATTCCCTTGTGCCATCCGTTATTg     >  1:202925/1‑251 (MQ=255)                                                                                                                                                                                                                                         tAATTATCATGG‑TCACATTTACTCA‑CA‑TT‑AATTCCCATGGGGAAGCCAATATGGTGGACGTTTCC‑GCCAAAGCCGAAACCGTCCGTGAAGCCCGCGCTGAGGCTTATGTTTGTATGGCGCCCGAAACCTTACAAATGATCCTGTCCGGTCAGCATCACAAAGGCGATGTGTTCGCCACCGCCCGCATTGCCGGCATTCAGGCGGCAAAACGCACGTGGGAATTGATTCCCTTGTGCCATCCGTTATTGCtt  >  1:900931/1‑251 (MQ=255)                                                                                                                                                                                                                                              aTCATGG‑TCACATTTACTCA‑CA‑TT‑AATTCCCATGGGGAAGCCAATATGGTGGACGTTTCC‑GCCAAAGCCGAAACCGTCCGTGAAGCCCGCGCTGAGGCTTATGTTTGTAt                                                                                                                                          >  1:649995/1‑110 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   GAAATTGCACCTTTGTTTATTCGGCGAGGAAGGCATTGAATTACGCGATTTGTTACAATACGATAAGCAGCAACTCATTTTGCAATCAAGAATTTTTTCTGCGTTACAAGGTAAGCGTGAACATCATTATCTGCACATCGGCGATAGTGGCGTACGTAGTCATTTGGCAAGTATCGGCGGTTAATGCA‑CGCC‑G‑A‑TTTTGAAAAACAGCAAG‑AAAATCAACCGCACTTTAATTATCATGG‑CCACATTTACTCA‑CA‑TT‑AATTCCCATGGGGAAGCCAATATGGTGGACGTTTCC‑GCCAAAGCCGAAACCGTCCGTGAAGCCCGCGCTGAGGCTTATGTTTGTATGGCGCCCGAAACCTTACAAATGATCCTGTCCGGTCAGCATCACAAAGGCGATGTGTTCGCCACCGCCCGCATTGCCGGCATTCAGGCGGCAAAACGCACGTGGGAATTGATTCCCTTGTGCCATCCGTTATTGCTT  >  1/1181335‑1181812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑