Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,179,707	A→G	intergenic (+20127/+241230)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,179,707	0	A	G	100.0%	155.1 / NA	55	intergenic (+20127/+241230)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/29);  total (26/29)

ATTTTCTTGGCTTGGCTTTGTTGCAGTTGTTCAATGACTTCAGACAAGAGAATCGGCGGTAGAATGCTGGTTAAAAAACTGCCGGGACCGAATAAAATCACTTCAGCTTCTTTTAATGCGCTAATGGCTTCCGGCGTGGCTTGCACATAGGGAACAAGAAAAATGGATTTTGGTAGTTCGGTTAAGCTGTCCACGCTCACTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATAGCGGCGAGATGCACGGGCTGCTCCGACATTGGGATGATGAAGGATTTGACATCAAGCAAATCACGAATCAGATTAACCGCTTCCATCGGGCGAATATGTAAATCTTCCAATGCTTTTAGCATCAGATTGCCGAGATTGTGTCCCGCCAGTTCGCCGTTGCCGCTGAAGCGATATTCAAATAAGGCGGAGGCAGTGCTCGGCTGCGTAATGATTTGATTTAAACAAT  >  1/1179474‑1179933                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                   aTTTTCTTGGCTTGGCTTTGTTGCAGTTGTTCAATGACTTCAGACAAGAGAATCGGCGGTAGAATGCTGGTTAAAAAACTGCCGGGACCGAATAAAATCACTTCAGCTTCTTTTAATGCGCTAATGGCTTCCGGCGTGGCTTGCACATAGGGAACAAGAAAAATGGATTTTGGTAGTTCGGTTAAGCTGTCCACGCTCACTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACggg                                                                                                                                                                                                                   <  1:519608/251‑1 (MQ=255)       ttggcttggcTTTGTTGCAGTTGTTCAATGACTTCAGACAAGAGAATCGGCGGTAGAATGCTGGTTAAAAAACTGCCGGGACCGAATAAAATCACTTCAGCTTCTTTTAATGCGCTAATGGCTTCCGGCGTGGCTTGCACATAGGGAACAAGAAAAATGGATTTTGGTAGTTCGGTTAAGCTGTCCACGCTCACTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACGGGCTGCTc                                                                                                                                                                                                             <  1:34309/251‑1 (MQ=255)       ttggcttggcTTTGTTGCAGTTGTTCAATGACTTCAGACAAGAGAATCGGCGGTAGAATGCTGGTTAAAAAACTGCCGGGACCGAATAAAATCACTTCAGCTTCTTTTAATGCGCTAATGGCTTCCGGCGTGGCTTGCACATAGGGAACAAGAAAAATGGATTTTGGTAGTTCGGTTAAGCTGTCCACGCTCACTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACGGGCTGCTc                                                                                                                                                                                                             <  1:332154/251‑1 (MQ=255)         ggcttggcttTGTTGCAGTTGTTCAATGACTTCAGACAAGAGAATCGGCGGTAGAATGCTGGTTAAAAAACTGCCGGGACCGAATAAAATCACTTCAGCTTCTTTTAATGCGCTAATGGCTTCCGGCGTGGCTTGCACATAGGGAACAAGAAAAATGGATTTTGGTAGTTCGGTTAAGCTGTCCACGCTCACTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACGGGCTGCTCCg                                                                                                                                                                                                           >  1:353660/1‑251 (MQ=255)                 tttGTTGCAGTTGTTCAATGACTTCAGACAAGAGAATCGGCGGTAGAATGCTGGTTAAAAAACTGCCGAGACCGAATAAAATCACTTCAGCTTCTTTTAATGCGCTAATGGCTTCCGGCGTGGCTTGCACATAGGGAACAAGAAAAATGGATTTTGGTAGTTCGGTTAAGCTGTCCACGCTCACTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCGCGGGCTGCTCCGACATTggg                                                                                                                                                                                                   <  1:463869/251‑1 (MQ=255)                   tgttgCAGTTGTTCAATGACTTCAGACAAGAGAATCGGCGGTAGAATGCTGGTTAAAAAACTGCCGGGACCGAATAAAATCACTTCAGCTTCTTTTAATGCGCTAATGGCTTCCGGCGTGGCTTGCACATAGGGAACAAGAAAAATGGATTTTGGTAGTTCGGTTAAGCTGTCCACGCTCACTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACGGGCTGCTCCGACATTGGgat                                                                                                                                                                                                 <  1:378260/251‑1 (MQ=255)                   tgttgCAGTTGTTCAATGACTTCAGACAAGAGAATCGGCGGTAGAATGCTGGTTAAAAAACTGCCGGGACCGAATAAAATCACTTCAGCTTCTTTTAATGCGCTAATGGCTTCCGGCGTGGCTTGCACATAGGGAACAAGAAAAATGGATTTTGGTAGTTCGGTTAAGCTGTCCACGCTCACTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACGGGCTGCTCCGACATTGGgat                                                                                                                                                                                                 >  1:245270/1‑251 (MQ=255)                    gttgCAGTTGTTCAATGACTTCAGACAAGAGAATCGGCGGTAGAATGCTGGTTAAAAAACTGCCGGGACCGAATAAAATCACTTCAGCTTCTTTTAATGCGCTAATGGCTTCCGGCGTGGCTTGCACATAGGGAACAAGAAAAATGGATTTTGGTAGTTCGGTTAAGCTGTCCACGCTCACTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACGGGCTGCTCCGACCTTGGgatg                                                                                                                                                                                                >  1:214605/1‑251 (MQ=255)                          gttgttCAATGACTTCAGACAAGAGAATCGGCGGTAGAATGCTGGTTAAAAAACTGCCGGGACCGAATAAAATCACTTCAGCTTCTTTTAATGCGCTAATGGCTTCCGGCGTGGCTTGCACATAGGGAACAAGAAAAATGGATTTTGGTAGTTCGGTTAAGCTGTCCACGCTCACTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACggg                                                                                                                                                                                                                   <  1:128844/226‑1 (MQ=255)                            tgttCAATGACTTCAGACAAGAGAATCGGCGGTAGAATGCTGGTTAAAAAACTGCCGGGACCGAATAAAATCACTTCAGCTTCTTTTAATGCGCTAATGGCTTCCGGCGTGGCTTGCACATAGGGAACAAGAAAAATGGATTTTGGTAGTTCGGTTAAGCTGTCCACGCTCACTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACGGGCTGCTCCGACATTGGGATGATGAAGGa                                                                                                                                                                                        <  1:531771/251‑1 (MQ=255)                            tgttCAATGACTTCAGACAAGAGAATCGGCGGTAGAATGCTGGTTAAAAAACTGCCGGGACCGAATAAAATCACTTCAGCTTCTTTTAATGCGCTAATGGCTTCCGGCGTGGCTTGCACATAGGGAACAAGAAAAATGGATTTTGGTAGTTCGGTTAAGCTGTCCACGCTCACTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACGGGCTGCTCCGACATTGGGATGATGAAGGa                                                                                                                                                                                        <  1:254502/251‑1 (MQ=255)                             gttCAATGACTTCAGACAAGAGAATCGGCGGTAGAATGCTGGTTAAAAAACTGCCGGGACCGAATAAAATCACTTCAGCTTCTTTTAATGCGCTAATGGCTTCCGGCGTGGCTTGCACATAGGGAACAAGAAAAATGGATTTTGGTAGTTCGGTTAAGCTGTCCACGCTCACTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACGGGCTGCTCCGACATTGGga                                                                                                                                                                                                  >  1:510872/1‑240 (MQ=255)                                  aTGACTTCAGACAAGAGAATCGGCGGTAGAATGCTGGTTAAAAAACTGCCGGGACCGAATAAAATCACTTCAGCTTCTTTTAATGCGCTAATGGCTTCCGGCGTGGCTTGCACATAGGGAACAAGAAAAATGGATTTTGGTAGTTCGGTTAAGCTGTCCACGCTCACTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACGGGCTGCTCCGACATTGGGATGATGAAGGATTTGAc                                                                                                                                                                                  <  1:116415/251‑1 (MQ=255)                                   tGACTTCAGACAAGAGAATCGGCGGTAGAATGCTGGTTAAAAAACTGCCGGGACCGAATAAAATCACTTCAGCTTCTTTTAATGCGCTAATGGCTTCCGGCGTGGCTTGCACATAGGGAACAAGAAAAATGGATTTTGGTAGTTCGGTTAAGCTGTCCACGCTCACTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACGGGCTGCTCCGACATTGGGATGATGAAGGATTTGACa                                                                                                                                                                                 <  1:561510/251‑1 (MQ=255)                                     aCTTCAGACAAGAGAATCGGCGGTAGAATGCTGGTTAAAAAACTGCCGGGACCGAATAAAATCACTTCAGCTTCTTTTAATGCGCTAATGGCTTCCGGCGTGGCTTGCACATAGGGAACAAGAAAAATGGATTTTTGTAGTTCGGTTAAGCTGTCCACGCTCACTTCGCCTACCACGCCGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACGGGCCGCTCCGACATTGGGATGATGAAGGATTTGACAt                                                                                                                                                                                >  1:47959/1‑250 (MQ=255)                                      cTTCAGACAAGAGAATCGGCGGTAGAATGCTGGTTAAAAAACTGCCGGGACCGAATAAAATCACTTCAGCTTCTTTTAATGCGCTAATGGCTTCCGGCGTGGCTTGCACATAGGGAACAAGAAAAATGGATTTTGGTAGTTCGGTTAAGCTGTCCACGCTCACTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACGGGCTGCTCCGACATTGGgatga                                                                                                                                                                                               <  1:790430/234‑1 (MQ=255)                                             cAAGAGAATCGGCGGTAGAATGCTGGTTAAAAAACTGCCGGGACCGAATAAAATCACTTCAGCTTCTTTTAATGCGCTAATGGCTTCCGGCGTGGCTTGCACATAGGGAACAAGAAAAATGGATTTTGGTAGTTCGGTTAAGCTGTCCACGCTCACTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACGGGCTGCTCCGACATTGGGATGATGAAGGAt                                                                                                                                                                                       >  1:1132/1‑235 (MQ=255)                                                gagaATCGGCGGTAGAATGCTGGTTAAAAAACTGCCGGGACCGAATAAAATCACTTCAGCTTCTTTTAATGCGCTAATGGCTTCCGGCGTGGCTTGCACATAGGGAACAAGAAAAATGGATTTTGGTAGTTCGGTTAAGCTGTCCACGCTCACTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACGGGCTGCTCCGACATTGGGatgatg                                                                                                                                                                                             <  1:509068/226‑1 (MQ=255)                                                      cggcggTAGAATGCTGGTTAAAAAACTGCCGGGACCGAATAAAATCACTTCAGCTTCTTTTAATGCGCTAATGGCTTCCGGCGTGGCTTGCACATAGGGAACAAGAAAAATGGATTTTGGTAGTTCGGTTAAGCTGTCCACGCTCACTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACGGGCTGCTCCGACATTGGGATGATGAAGGATTTGACATCAAGc                                                                                                                                                                           >  1:73299/1‑238 (MQ=255)                                                           gTAGAATGCTGGTTAAAAAACTGCCGGGACCGAATAAAATCACTTCAGCTTCTTTTAATGCGCTAATGGCTTCCGGCGTGGCTTGCACATAGGGAACAAGAAAAATGGATTTTGGTAGTTCGGTTAAGCTGTCCACGCTCACTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACGGGCTGCTCCGACATTGGga                                                                                                                                                                                                  <  1:473768/210‑1 (MQ=255)                                                                                 gCCGGGACCGAATAAAATCACTTCAGCTTCTTTTAATGCGCTAATGGCTTCCGGCGTGGCTTGCACATAGGGAACAAGAAAAATGGATTTTGGTAGTTCGGTTAAGCTGTCCACGCTCACTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACGGGCTGCTCCGACATTGGGATGATGAAGGATTTGACATCAAGCAAATCACGAATCAGATTAACCGCTTCCATCGGGCGAatat                                                                                                                                   >  1:900692/1‑251 (MQ=255)                                                                                        ccGAATAAAATCACTTCAGCTTCTTTTAATGCGCTAATGGCTTCCGGCGTGGCTTGCACATAGGGAACAAGAAAAATGGATTTTGGTAGTTCGGTTAAGCTGTCCACGCTCACTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACGGGCTGCTCCGACATTGGGATGATGAAGGATTTGACATCAAGCAAATCACGAATCAGATTAACCGCTTCCATCGGGCGAATATGTAAATc                                                                                                                            <  1:474482/251‑1 (MQ=255)                                                                                           aaTAAAATCACTTCAGCTTCTTTTAATGCGCTAATGGCTTCCGGCGTGGCTTGCACATAGGGAACAAGAAAAATGGATTTTGGTAGTTCGGTTAAGCTGTCCACGCTCACTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACGGGCTGCTCCGACATTGGGATGATGAAGGATTTGACATCAAGCAAATCACGAATCAGATTAACCGCTTCCATCGGGCGAATATGTAAATCtt                                                                                                                          >  1:613006/1‑250 (MQ=255)                                                                                           aaTAAAATCACTTCAGCTTCTTTTAATGCGCTAATGGCTTCCGGCGTGGCTTGCACATAGGGAACAAGAAAAATGGATTTTGGTAGTTCGGTTAAGCTGTCCACGCTCACTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACGGGCTGCTCCGACATTGGGATGATGAAGGATTTGACATCAAGCAAATCACGAATCAGATTAACCGCTTCCATCGGGCGAATATGTAAATCtt                                                                                                                          >  1:589021/1‑250 (MQ=255)                                                                                                   cACTTCAGCTTCTTTTAATGCGCTAATGGCTTCCGGCGTGGCTTGCACATAGGGAACAAGAAAAATGGATTTTGGTAGTTCGGTTAAGCTGTCCACGCTCACTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACGGGCTGCTCCGACATTGGGATGATGAAGGATTTGACATCAAGCAAATCACGAATCAGATTAACCGCTTCCATCGGGCGAATATGTAAATCTTCCAAt                                                                                                                     >  1:463617/1‑247 (MQ=255)                                                                                                                       cgcTAATGGCTTCCGGCGTGGCTTGCACATAGGGAACAAGAAAAATGGATTTTGGTAGTTCGGTTAAGCTGTCCACGCTCACTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACGGGCTGCTCCGACATTGGGATGATGAAGGATTTGACATCAAGCAAATCACGAATCAGATTAACCGCTTCCATCGGGCGAATATGTAAATCTTCCAATGCtt                                                                                                                 <  1:351802/231‑1 (MQ=255)                                                                                                                       cgcTAATGGCTTCCGGCGTGGCTTGCACATAGGGAACAAGAAAAATGGATTTTGGTAGTTCGGTTAAGCTGTCCACGCTCACTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACGGGCTGCTCCGACATTGGGATGATGAAGGATTTGACATCAAGCAAATCACGAATCAGATTAACCGCTTCCATCGGGCGAATATGTAAATCTTCCAATGCTTTTAGc                                                                                                            >  1:671163/1‑236 (MQ=255)                                                                                                                       cgcTAATGGCTTCCGGCGTGGCTTGCACATAGGGAACAAGAAAAATGGATTTTGGTAGTTCGGTTAAGCTGTCCACGCTCACTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACGGGCTGCTCCGACATTGGGATGATGAAGGATTTGACATCAAGCAAATCACGAATCAGATTAACCGCTTCCATCGGGCGAATATGTAAATCTTCCAATGCTTTTAGCATCAGATTGCCgaga                                                                                             >  1:893863/1‑251 (MQ=255)                                                                                                                         cTAATGGCTTCCGGCGTGGCTTGCACATAGGGAACAAGAAAAATGGATTTTGGTAGTTCGGTTAAGCTGTCCACGCTCACTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATGGCGGCGCGATGCACGGGCTGCTCCGACATTGGGATGATGAAGGATTTGACATCAAGCAAATCACGAATCAGATTAACCGCTTCCATCGGGCGAATATGTAAATCTTCCAATGCTTTTAGCATCAGATTGCCGAGAtt                                                                                           >  1:787250/1‑251 (MQ=255)                                                                                                                           aaTGGCTTCCGGCGTGGCTTGCACATAGGGAACAAGAAAAATGGATTTTGGTAGTTCGGTTAAGCTGTCCACGCTCACTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACGGGCTGCTCCGACATTGGGATGATGAAGGATTTGACATCAAGCAAATCACGAATCAGATTAACCGCTTCCATCGGGCGAATATGTAAATCTTCCAATGCTTTTAGCATCAGATTGCCGAGATtgt                                                                                         >  1:643848/1‑251 (MQ=255)                                                                                                                              ggCTTCCGGCGTGGCTTGCACATAGGGAACAAGAAAAATGGATTTTGGTAGTTCGGTTAAGCTGTCCACGCTCACTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACGGGCTGCTCCGACATTGGGATGATGAAGGATTTGACATCAAGCAAATCACGAATCAGATTAACCGCTTCCATCGGGCGAATATGTAAATCTTCCAATGCTTTTAGCATCAGATTGCCGAGATTgtgt                                                                                       >  1:687230/1‑250 (MQ=255)                                                                                                                                   ccGGCGTGGCTTGCACATAGGGAACAAGAAAAATGGATTTTGGTAGTTCGGTTAAGCTGTCCACGCTCACTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACGGGCTGCTCCGACATTGGGATGATGAAGGATTTGACATCAAGCAAATCACGAATCAGATTAACCGCTTCCATCGGGCGAATATGTAAATCTTCCAATGCTTTTAGCATCAGATTGCCGAGATTGTGTCCCGcc                                                                                 <  1:566211/251‑1 (MQ=255)                                                                                                                                          ggCTTGCACATAGGGAACAAGAAAAATGGATTTTGGTAGTTCGGTTAAGCTGTCCACGCTCACTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACGGGCTGCTCCGACATTGGGATGATGAAGGATTTGACATCAAGCAAATCACGAATCAGATTAACCGCTTCCATCGGGCGAATATGTAAATCTTCCAATGCTTTTAGCATc                                                                                                         >  1:337762/1‑220 (MQ=255)                                                                                                                                               gCACATAGGGAACAAGAAAAATGGATTTTGGTAGTTCGGTTAAGCTGTCCACGCTCACTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACGGGCTGCTCCGACATTGGGATGATGAAGGATTTGACa                                                                                                                                                                                 <  1:253403/143‑1 (MQ=255)                                                                                                                                               gCACATAGGGAACAAGAAAAATGGATTTTGGTAGTTCGGTTAAGCTGTCCACGCTCACTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACGGGCTGCTCCGACATTGGGATGATGAAGGATTTGACATCAAGCAAATCACGAATCAGATTAACCGCTTCCATCGGGCGAATATGTAAATCTTCCAATGCTTTTAGCATCAGATTGCCGAGATTGTGTCCCGCCAGTTCGCCGTTg                                                                     >  1:646387/1‑251 (MQ=255)                                                                                                                                               gCACATAGGGAACAAGAAAAATGGATTTTGGTAGTTCGGTTAAGCTGTCCACGCTCACTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACGGGCTGCTCCGACATTGGGATGATGAAGGATTTGACATCAAGCAAATCACGAATCAGATTAACCGCTTCCATCGGGCGAATATGTAAATCTTCCAATGCTTTTAGCATCAGATTGCCGAGATTGTGTCCCGCCAGTTCGCCGTTg                                                                     >  1:61868/1‑251 (MQ=255)                                                                                                                                                cacaTAGGGAACAAGAAAAATGGATTTTGGTAGTTCGGTTAAGCTGTCCACGCTCACTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACGGGCTGCTCCGACATTGGGATGATGAAGGATTTGACATCAAGCAAATCACGAATCAGATTAACCACTTCCATCGGGCGAata                                                                                                                                    >  1:451205/1‑187 (MQ=255)                                                                                                                                                         aaCAAGAAAAATGGATTTTGGTAGTTCGGTTAAGCTGTCCACGCTCACTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACGGGCTGCTCCGACATTGGGATGATGAAGGATTTGACATCAAGCAAATCACGAATCAGATTAACCGCTTCCATCGGGCGAATATGTAAATCTTCCAATGCTTTTAGCATCAGATTGCCGAGATTGTGTCCCGCCAGTTCGCCGTTGCCGCTGAAg                                                            <  1:288677/250‑1 (MQ=255)                                                                                                                                                         aaCAAGAAAAATGGATTTTGGTAGTTCGGTTAAGCTGTCCACGCTCACTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACGGGCTGCTCCGACATTGGGATGATGAAGGATTTGACATCAAGCAAATCACGAATCAGATTAACCGCTTCCATCGGGCGAATATGTAAATCTTCCAATGCTTTTAGCATCAGATTGCCGAGATTGTGTCCCGCCAGTTCGCCGTTGCCGCTGAAg                                                            <  1:856678/250‑1 (MQ=255)                                                                                                                                                                                 ttCGGGTAAGCTGTCCACGCTCTCTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACGGGCTGCTCCGACATTGGGATGATGAAGGATTTGACATCAAGCAAATCACGAATCAGATTAACCGCTTCCATCGGGCGAATATGTAAATCTTCCAATGCTTTTAGCATCAGATTGCCGAGATTGTGTCCCGCCAGTTCGCCGTTGCCGCTGAAGCGATATTCAAATAAGGCGGAGGc                                     <  1:421808/249‑1 (MQ=255)                                                                                                                                                                                     gTTAAGCTGTCCACGCTCACTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACGGGCTGCTCCGACATTGGGATGATGAAGGATTTGAc                                                                                                                                                                                  <  1:262453/104‑1 (MQ=255)                                                                                                                                                                                     gTTAAGCTGTCCACGCTCACTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACGGGCTGCTCCGACATTGGGATGATGAAGGATTTGACATCAAGCAAATCACGAATCAGATTAACCGCTTCCATCGGGCGAATATGTAAATCTTCCAATGCTTTTAGCATCAGATTGCCGAGAt                                                                                            >  1:633828/1‑190 (MQ=255)                                                                                                                                                                                     gTTAAGCTGTCCACGCTCACTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACGGGCTGCTCCGACATTGGGATGATGAAGGATTTGACATCAAGCAAATCACGAATCAGATTAACCGCTTCCATCGGGCGAATATGTAAATCTTCCAATGCTTTTAGCATCAGATTGCCGAGATTGTGTCCCGCCAGTTCGCCGTTGCCGCTGAAGCGATATTCAAATAAGGCGGAGGCAGTg                                 >  1:255643/1‑249 (MQ=255)                                                                                                                                                                                      ttAAGCTGTCCACGCTCACTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACGGGCTGCTCCGACATTGGGATGATGAAGGATTTGACATCAAGCAAATCACGAATCAGATTAACCGCTTCCATCGGGCGAATATGTAAATCTTCCAATGCTTTTAGCATCAGATTGCCGAGATTGTGTCCCGCCAGTTCGCCGTTGCCGCTGAAGCGATATTCAAATAAGGCGGAGGCAGTGCt                               <  1:529098/250‑1 (MQ=255)                                                                                                                                                                                           cTGTCCACGCTCACTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACGGGCTGCTCCGACATTGGGATGATGAAGGATTTGACATCAAGCAAATCACGAATCAGATTAACCGCTTCCATCGGGCGAATATGTAAATCTTCCAATGCTTTTAGCATCAGATTGCCGAGATTGTGTCCCGCCAGTTCGCCGTTGCCGCTGAAGCGATATTCAAATAAGGCGGAGGCAGTGCTCGGc                           <  1:838601/249‑1 (MQ=255)                                                                                                                                                                                           cTGTCCACGCTCACTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACGGGCTGCTCCGACATTGGGATGATGAAGGATTTGACATCAAGCAAATCACGAATCAGATTAACCGCTTCCATCGGGCGAATATGTAAATCTTCCAATGCTTTTAGCATCAGATTGCCGAGATTGTGTCCCGCCAGTTCGCCGTTGCCGCTGAAGCGATATTCAAATAAGGCGGAGGCAGTGCTCGGc                           <  1:183278/249‑1 (MQ=255)                                                                                                                                                                                             gTCCACGCTCACTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACGGGCTGCTCCGACATTGGGATGATGAAGGATTTGACATCAAGCAAATCACGAATCAGATTAACCGCTTCCATCGGGCGAATATGTAAATCTTCCAATGCTTTTAGCATCGGATTGCCGAGATTGTGTCCCGCCAGTTCGCCGTTGCCGCTGAAGCGATATTCAAATAAGGCGGAGGCAGTGc                                >  1:433402/1‑242 (MQ=255)                                                                                                                                                                                             gTCCACGCTCACTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACGGGCTGCTCCGACATTGGGATGATGAAGGATTTGACATCAAGCAAATCACGAATCAGATTAACCGCTTCCATCGGGCGAATATGTAAATCTTCCAATGCTTTTAGCATCAGATTGCCGAGATTGTGTCCCGCCAGTTCGCCGTTGCCGCTGAAGCGATATTCAAATAAGGCGGAGGCAGTGCTCGGCTGCg                       >  1:413388/1‑251 (MQ=255)                                                                                                                                                                                              tCCACGCTCACTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACGGGCTGCTCCGACATTGGGATGATGAAGGATTTGACATCAAGCAAATCACGAATCAGATTAACCGCTTCCATCGGGCGAATATGTAAATCTTCCAATGCTTTTAGCATCAGATTGCCGAGATTGTGTCCCGCCAGTTCGCCGTTGCCGCTGAAGCGATATTCAAATAAGGCGGAGGCAGTGCTCGGCTGCGt                      >  1:779721/1‑251 (MQ=255)                                                                                                                                                                                                cACGCTCGCTTCGCCTGCCACGCTGGCGCCGCTGCCTGAAATGGCGGCGGGATGCACGGGCTGCTCCGACATTGGGATGATGAAGGATTTGACATCAAGCAAATCACGAATCAGATTAACCGCTTCCATCGGGCGAATATGTAAATCTTCCAATGCTTTTAGCATCAGATTGCCGAGATTGTGTCCCGCCAGTTCGCCGTTGCCGCTGAAGCGATATTCAAATAAGGCGGAGGCAGTGCTCGGCTGCGTaa                    <  1:226666/251‑1 (MQ=255)                                                                                                                                                                                                      cACTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACGGGCTGCTCCGACATTGGGATGATGAAGGATTTGACATCAAGCAAATCACGAATCAGATTAACCGCTTCCATCGGGCGAATATGTAAATCTTCCAATGCTTTTAGCATCAGATTGCCGAGATTGTGTCCCGCCAGTTCGCCGTTGCCGCTGAAGCGATATTCAAATAAGGCGGAGGCAGTGCTc                              <  1:694468/235‑1 (MQ=255)                                                                                                                                                                                                        cTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACGGGCTGCTCCGACATTGGGATGATGAAGGATTTGACATCAAGCAAATCACGAATCAGATTAACCGCTTCCATCGGGCGAATATGTAAATCTTCCAATGCTTTTAGCATCAGATTGCCGAGAtt                                                                                           >  1:115699/1‑172 (MQ=255)                                                                                                                                                                                                                  cACGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACGGGCTGCTCCGACATTGGGATGATGAAGGATTTGACATCAAGCAAATCACGAATCAGATTAACCGCTTCCATCGGGCGAATATGTAAATCTTCCAATGCTTTTAGCATCAGATTGCCGAGATTGTGTCCCGCCAGTTCGCCGTTGCCGCTGAAGCGATATTCAAATAAGGCGGAGGCAGTGCTCGGCTGCGTAATGATTTGATTTAAACAAt  <  1:48076/251‑1 (MQ=255)                                                                                                                                                                                                                    cGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACGGGCTGCTCCGACATTGGGATGATGAAGGATTTGACATCAAGCAAATCACGAATCAGATTAACCGCTTCCATCGGGCGAATATGTAAATCTTCCAATGCTTTTAGCATCAGATTGCCGAGATTGTGTCCCGCCAGTTCGCCGTTGCCGCTGAAGCGATATTCAAATa                                               <  1:848032/204‑1 (MQ=255)                                                                                                                                                                                                                    cGCTGGCGCCGCTGCCTGAAATGGCGGCGAGATGCACGGGCTGCTCCGACATTGGGATGATGAAGGATTTGACATCAAGCAAATCACGAATCAGATTAACCGCTTCCATCGGGCGAATATGTAAATCTTCCAATGCTTTTAGCATCAGATTGCCGAGATTGTGTCCCGCCAGTTCGCCGTTGCCGCTGAAGCGATATTCAAATAAGGc                                           <  1:512357/208‑1 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                   ATTTTCTTGGCTTGGCTTTGTTGCAGTTGTTCAATGACTTCAGACAAGAGAATCGGCGGTAGAATGCTGGTTAAAAAACTGCCGGGACCGAATAAAATCACTTCAGCTTCTTTTAATGCGCTAATGGCTTCCGGCGTGGCTTGCACATAGGGAACAAGAAAAATGGATTTTGGTAGTTCGGTTAAGCTGTCCACGCTCACTTCGCCTACCACGCTGGCGCCGCTGCCTGAAATAGCGGCGAGATGCACGGGCTGCTCCGACATTGGGATGATGAAGGATTTGACATCAAGCAAATCACGAATCAGATTAACCGCTTCCATCGGGCGAATATGTAAATCTTCCAATGCTTTTAGCATCAGATTGCCGAGATTGTGTCCCGCCAGTTCGCCGTTGCCGCTGAAGCGATATTCAAATAAGGCGGAGGCAGTGCTCGGCTGCGTAATGATTTGATTTAAACAAT  >  1/1179474‑1179933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑