Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,178,769	G→A	intergenic (+19189/+242168)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,178,769	0	G	A	100.0%	204.0 / NA	71	intergenic (+19189/+242168)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (44/27);  total (44/27)

GTGATAGGTTTGCTTGGGCAGACCTTGAGTAATCAATCTTTGTTATCTGACGAAGGCAAAGCCGTATTGTCTGTTGTGCAGGATTATGCTAGGAGTTGGAGTTTGTTACAGGCTTACGATGAGCAAAGTCTAACGGCTAATCAGCATAAGCAAAATAATATGGTTCTGTTGGTGTTAGATGACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACGGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTACCACGATTGAACAAGGATTCGGTGACACGCTTTTTTATCCGAATGTTGCCAGCCGAGCGGCGAATTTATTGTATTTTATTATCAAAAATCATCCGTTGACCGATGGCAATAAACGCACGGGATCTTTCCTGTTTTTATGGTATTTGCGGTTGAATCAACACTTATTGGCAAGGCCGGTAGAACAATTGATTAATGACAAT  >  1/1178530‑1179015                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                       gTGATAGGTTTGCTTGGGCAGACCTTGAGTAATCAATCTTTGTTATCTGACGAAGGCAAAGCCGTATTGTCTGTTGTGCAGGATTATGCTAGGAGTTGGAGTTTGTTACAGGCTTACGATGAGCAAAGTCTAACGGCTAATCAGCATAAGCAAAATAATATGGTTCTGTTGGTGTTAGATGACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCgttgt                                                                                                                                                                                                                                                 >  1:172635/1‑247 (MQ=255)    aTAGGTTTGCTTGGGCAGACCTTGAGTAATCAATCTTTGTTATCTGACGAAGGCAAAGCCGTATTGTCTGTTGTGCAGGATTATGCTAGGAGTTGGAGTTTGTTACAGGCTTACGATGAGCAAAGTCTAACGGCTAATCAGCATAAGCAAAATAATATGGTTCTGTTGGTGTTAGATGACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGac                                                                                                                                                                                                                                           <  1:74566/250‑1 (MQ=255)            gCTTGGGCAGACCTTGAGTAATCAATCTTTGTTATCTGACGAAGGCAAAGCCGTATTGTCTGTTGTGCAGGATTATGCTAGGAGTTGGTGTTTGTTACAGGCTTACGATGAGCAAAGTCTAACGGCTAATCAGCATAAGCAAAATACTATGGTTCTGTTGGTGTTACATAACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGCCAACAACGaa                                                                                                                                                                                                                                  >  1:431641/1‑251 (MQ=255)                     gACCTTGAGTAATCAATCTTTGTTATCTGACGAAGGCAAAGCCGTATTGTCTGTTGTGCAGGATTATGCTAGGAGTTGGAGTTTGTTACAGGCTTACGATGAGCAAAGTCTAACGGCTAATCAGCATAAGCAAAATAATATGGTTCTGTTGGTGTTAGATGACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGtt                                                                                                                                                                                                                         >  1:277146/1‑251 (MQ=255)                          tGAGTAATCAATCTTTGTTATCTGACGAAGGCAAAGCCGTATTGTCTGTTGTGCAGGATTATGCTAGGAGTTGGAGTTTGTTACAGGCTTACGATGAGCAAAGTCTAACGGCTAATCAGCATAAGCAAAATAATATGGTTCTGTTGGTGTTAGATGACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGcc                                                                                                                                                                                                                    <  1:153483/251‑1 (MQ=255)                          tGAGTAATCAATCTTTGTTATCTGACGAAGGCAAAGCCGTATTGTCTGTTGTGCAGGATTATGCTAGGAGTTGGAGTTTGTTACAGGCTTACGATGAGCAAAGTCTAACGGCTAATCAGCATAAGCAAAATAATATGGTTCTGTTGGTGTTAGATGACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGcc                                                                                                                                                                                                                    <  1:318235/251‑1 (MQ=255)                                  caatcTTTGTTATCTGACGAAGGCAAAGCCGTATTGTCTGTTGTGCAGGATTATGCTAGGAGTTGGAGTTTGTTACAGGCTTACGATGAGCAAAGTCTAACGGCTAATCAGCATAAGCAAAATAATATGGTTCTGTTGGTGTTAGATGACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCAt                                                                                                                                                                                                            >  1:763339/1‑251 (MQ=255)                                      cTTTGTTATCTGACGAAGGCAAAGCCGTATTGTCTGTTGTGCAGGATTATGCTAGGAGTTGGAGTTTGTTACAGGCTTACGATGAGCAAAGTCTAACGGCTAATCAGCATAAGCAAAATAATATGGTTCTGTTGGTGTTAGATGACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCAttgc                                                                                                                                                                                                         >  1:710472/1‑248 (MQ=255)                                                 gACGAAGGCAAAGCCGTATTGTCTGTTGTGCAGGATTATGCTAGGAGTTGGAGTTTGTTACAGGCTTACGATGAGCAAAGTCTAACGGCTAATCAGCATAAGCAAAATAATATGGTTCTGTTGGTGTTAGATGACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACa                                                                                                                                                                                             >  1:272966/1‑251 (MQ=255)                                                  aCGAAGGCAAAGCCGTATTGTCTGTTGTGCAGGATTATGCTAGGAGTTGGAGTTTGTTACAGGCTTACGATGAGCAAAGTCTAACGGCTAATCAGCATAAGCAAAATAATATGGTTCTGTTGGTGTTAGATGACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACaa                                                                                                                                                                                            <  1:792670/251‑1 (MQ=255)                                                     aaGGCAAAGCCGTATTGTCTGTTGTGCAGGATTATGCTAGGAGCTGGAGTTTGTTACAGGCTTACGATGAGCAAAGTCTAACGGCTAATCAGCATAAGCAAAATAATATGGTTCTGTTGGTGTTAGATGACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGa                                                                                                                                                                                         <  1:527664/251‑1 (MQ=255)                                                             gCCGTATTGTCTGTTGTGCAGGATTATGCTAGGAGTTGGAGTTTGTTACAGGCTTACGATGAGCAAAGTCTAACGGCTAATCAGCATAAGCAAAATAATATGGTTCTGTTGGTGTTAGATGACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCAttgcc                                                                                                                                                                                                        >  1:210457/1‑225 (MQ=255)                                                             gCCGTATTGTCTGTTGTGCAGGATTATGCTAGGAGTTGGAGTTTGTTACAGGCTTACGATGAGCAAAGTCTAACGGCTAATCAGCATAAGCAAAATAATATGGTTCTGTTGGTGTTAGATGACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGATTCGGTGa                                                                                                                                                                                 >  1:269498/1‑251 (MQ=255)                                                                        tgttgtGCAGGATTATGCTAGGAGTTGGAGTTTGTTACAGGCTTACGATGAGCAAAGTCTAACGGCTAATCAGCATAAGCAAAATAATATGGTTCTGTTGGTGTTAGATGACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGATTCGGTGACACGCtttttt                                                                                                                                                                      <  1:274131/251‑1 (MQ=255)                                                                         gttgtGCAGGATTATGCTAGGAGTTGGAGTTTGTTACAGGCTTACGATGAGCAAAGTCTAACGGCTAATCAGCATAAGCAAAATAATATGGTTCTGTTGGTGTTAGATGACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGa                                                                                                                                                                                         >  1:890863/1‑231 (MQ=255)                                                                            gtgCAGGATTATGCTAGGAGTTGGAGTTTGTTACAGGCTTACGATGAGCAAAGTCTAACGGCTAATCAGCATAAGCAAAATAATATGGTTCTGTTGGTGTTAGATGACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGATTCGGTGACACGCTTTTTTATc                                                                                                                                                                   >  1:848744/1‑250 (MQ=255)                                                                                 ggATTATGCTAGGAGTTGGAGTTTGTTACAGGCTTACGATGAGCAAAGTCTAACGGCTAATCAGCATAAGCAAAATAATATGGTTCTGTTGGTGTTAGATGACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGAt                                                                                                                                                                                        <  1:543255/224‑1 (MQ=255)                                                                                  gATTATGCTAGGAGTTGGAGTTTGTTACAGGCTTACGATGAGCAAAGTCTAACGGCTAATCAGCATAAGCAAAATAATATGGTTCTGTTGGTGTTAGATGACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGATTCGGTGACACGCTTTTTTATCCGAATg                                                                                                                                                             <  1:768504/250‑1 (MQ=255)                                                                                   aTTATGCTAGGAGTTGGAGTTTGTTACAGGCTTACGATGAGCAAAGTCTAACGGCTAATCAGCATAAGCAAAATAATATGGTTCTGTTGGTGTTAGATGACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGATTCGGTGACACGCTTTTTTATCCGAATGtt                                                                                                                                                           >  1:24877/1‑251 (MQ=255)                                                                                             gagttggagttTGTTACAGGCTTACGATGAGCAAAGTCTAACGGCTAATCAGCATAAGCAAAATAATATGGTTCTGTTGGTGTTAGATGACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGATTCGGTGACACGCTTTTTTATCCGAATGTTGCCAGCCGAg                                                                                                                                                 >  1:796490/1‑251 (MQ=255)                                                                                                     gttTGTTACAGGCTTACGATGAGCAAAGTCTAACGGCTAATCAGCATAAGCAAAATAATATGGTTCTGTTGGTGTTAGATGACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGATTCGGTGACACGCTTTTTTATCCGAATGTTGCCAGCCGAGCGGCGAAt                                                                                                                                         >  1:877711/1‑251 (MQ=255)                                                                                                         gTTACAGGCTTACGATGAGCAAAGTCTAACGGCTAATCAGCATAAGCAAAATAATATGGTTCTGTTGGTGTTAGATGACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGAt                                                                                                                                                                                                   >  1:569253/1‑189 (MQ=255)                                                                                                               ggCTTACGATGAGCAAAGTCTAACGGCTAATCAGCATAAGCAAAATAATATGGTTCTGTTGGTGTTAGATGACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGATTCGGTGACACGCTTTTTTATCCGAATGTTGCCAGCCGAGCGGCGAATTTATTGTAtt                                                                                                                               >  1:634262/1‑251 (MQ=255)                                                                                                               ggCTTACGATGAGCAAAGTCTAACGGCTAATCAGCATAAGCAAAATAATATGGTTCTGTTGGTGTTAGATGACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTACCATTGCCACGATTGAACAAGGATTCGGTGACACGCTTTTTTATCCGAATGTTGCCAGCCGAGCGGCGAATTTATTGTAtt                                                                                                                               >  1:453119/1‑251 (MQ=255)                                                                                                                                gTCTAACGGCTAATCAGCATAAGCAAAATAATATGGTTCTGTTGGTGTTAGATGACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGAGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGcc                                                                                                                                                                                                                    <  1:119044/149‑1 (MQ=255)                                                                                                                                    aaCGGCTAATCAGCATAAGCAAAATAATATGGTTCTGTTGGTGTTAGATGACGTATTACAGGCAATTTCTCAATCAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGATTCGGTGACACGCTTTTTTATCCGAATGTTGCCAGCCGAGCGGCGAATTTATTGTATTTTATTATCAAAAATCATCCGt                                                                                                          <  1:569589/251‑1 (MQ=255)                                                                                                                                      cGGCTAATCAGCATAAGCAAAATAATATGGTTCTGTTGGTGTTAGATGACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACaa                                                                                                                                                                                            <  1:840134/167‑1 (MQ=255)                                                                                                                                      cGGCTAATCAGCATAAGCAAAATAATATGGTTCTGTTGGTGTTAGATGACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGATTCGGTGACACGCTTTTTTATCCGAATGTTGCCAGCCGAgcgg                                                                                                                                              >  1:467632/1‑213 (MQ=255)                                                                                                                                           aaTCAGCATAAGCAAAATAATATGGTTCTGTTGGTGTTAGATGACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGATTCGGTAacac                                                                                                                                                                              >  1:21824/1‑176 (MQ=255)                                                                                                                                                gCATAAGCAAAATAATATGGTTCTGTTGGTGTTAGATGACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGATTCGGTGACACGCTTTTTTATCCGAATGTTGCCAGCCGAGCGTCGAATTTATTGTATTTTATTATCAAAAATCATCCGTTGACCGATGGCa                                                                                              >  1:497704/1‑251 (MQ=255)                                                                                                                                                       cAAAATAATATGGTTCTGTTGGTGTTAGATGACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGATTCGGTGACACGCTTTTTTATCCGAATGTTGCCAGCCGAGCGGCGAATTTATTGTATTTTATTATCAAAAATCATCCGTTGACCGATGGCAATAAAc                                                                                        <  1:313659/250‑1 (MQ=255)                                                                                                                                                       cAAAATAATATGGTTCTGTTGGTGTTAGATGACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGATTCGGTGACACGCTTTTTTATCCGAATGTTGCCAGCCGAGCGGCGAATTTATTGTATTTTATTATCAAAAATCATCCGTTGACCGATGGCAATAAACg                                                                                       <  1:891714/251‑1 (MQ=255)                                                                                                                                                         aaaTAATATGGTTCTGTTGGTGTTAGATGACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCgttg                                                                                                                                                                                                                                                  >  1:801063/1‑94 (MQ=255)                                                                                                                                                         aaaTAATATGGTTCTGTTGGTGTTAGATGACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGcc                                                                                                                                                                                                                    >  1:289670/1‑124 (MQ=255)                                                                                                                                                         aaaTAATATGGTTCTGTTGGTGTTAGATGACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGATTCGGTGACACGCTTTTTTATCCGAATGTTGCCAGCCGAGCGGCGAATTTATTGTATTTTATTATCAAAAATCATCCGTTGACCGATGGCAATAAACg                                                                                       <  1:23182/249‑1 (MQ=255)                                                                                                                                                         aaaTAATATGGTTCTGTTGGTGTTAGATGACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGCGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGCAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGATTCGGTGACACGCTTTTTTATCCGAATGTTGCTAGCCGAGCTGCGAATTTATTGTATTttat                                                                                                                           >  1:185487/1‑213 (MQ=255)                                                                                                                                                           ataataTGGTTCTGTTGGTGTTAGATGACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGATTCGGTGACACGCTTTTTTATCCGAATGTTGCCAGCCGAGCGGCGAATTTATTGTATTTTATTATCAAAAATCATCCGTTGACCGATGGCAATAAACGCACg                                                                                   >  1:654778/1‑251 (MQ=255)                                                                                                                                                                   gTTCTGTTGGTGTTAGATGACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGATTCGGTGACACGCTTTTTTATCCGAATGTTGCCAGCCGAGCGGCGAATTTATTGTATTTTATTATCAAAAATCATCCGTTGACCGATgg                                                                                                <  1:466187/230‑1 (MQ=255)                                                                                                                                                                   gTTCTGTTGGTGTTAGATGACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGATTCGGTGACACGCTTTTTTATCCGAATGTTGCCAGCCGAGCGGCGAATTTATTGTATTTTATTATCAAAAATCATCCGTTGACCGATGGCAATAAACGCACGGGATCttt                                                                           >  1:767028/1‑251 (MQ=255)                                                                                                                                                                      cTGTTGGTGTTAGATGACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGATTCGGTGACACGCTTTTTTATCCGAATGTTGCCAGCCGAGCGGCGAATTTATTGTATTTTATTATCAAAAATCATCCGTTGACCGATGGCAATAAACGCACGGGATCTTTc                                                                          >  1:463568/1‑249 (MQ=255)                                                                                                                                                                       tGTTGGTGTTAGATGACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGATTCGGTGACACGCTTTTTTATCCGAATGTTGCCAGCCGAGCGGCGAATTTATTGTATTTTATTATCAAAAATCATCCGTTGACCGATGGCAATAAACGCACGGGATCTTTCCTg                                                                       <  1:489334/251‑1 (MQ=255)                                                                                                                                                                       tGTTGGTGTTAGATGACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGATTCGGTGACACGCTTTTTTATCCGAATGTTGCCAGCCGAGCGGCGAATTTATTGTATTTTATTATCAAAAATCATCCGTTGACCGATGGCAATAAACGCACGGGATCTTTCCTg                                                                       <  1:652429/251‑1 (MQ=255)                                                                                                                                                                        gTTGGTGTTAGATGACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGATTCGGTGACACGCtt                                                                                                                                                                          >  1:703540/1‑151 (MQ=255)                                                                                                                                                                        gTTGGTGTTAGATGACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGATTCGGTGACACGCTTTTTTATCCGAATGTTGCCAGCCGAGCGGCGAATTTATTg                                                                                                                                   >  1:215737/1‑190 (MQ=255)                                                                                                                                                                            gtgtTAGATGACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGATTCGGTGACACGCTTTTTTATCCGAATGTTGCCAGCCGAGCGGCGAATTTATTGTATTTTATTATCAAAAATCATCCGTTGACCGATGGCAATAAACGCACGGGATCTTTCCTGttttt                                                                  >  1:213860/1‑251 (MQ=255)                                                                                                                                                                            gtgtTAGATGACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGATTCGGTGACACGCTTTTTTATCCGAATGTTGCCAGCCGAGCGGCGAATTTATTGTATTTTATTATCAAAAATCATCCGTTGACCGATGGCAATAAACGCACGGGATCTTTCCTGttttt                                                                  >  1:417930/1‑251 (MQ=255)                                                                                                                                                                            gtgtTAGATGACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGATTCGGTGACACGCTTTTTTATCCGAATGTTGCCAGCCGAGCGGCGAATTTATTGTATTTTATTATCAAAAATCATCCGTTGACCGATGGCAATAAACGCACGGGATCTTTCCTGttttt                                                                  >  1:552502/1‑251 (MQ=255)                                                                                                                                                                                tAGATGACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGATTCGGTGACACGCTTTTTTATCCGAATGTTGCCAGCCGAGCGGCGAATTTATTGTATTTTATTATCAAAAATCATCCGTTGACCGATGGCAATAAACGCACGGGATCTTTCCTGTTTTTATgg                                                              <  1:456425/251‑1 (MQ=255)                                                                                                                                                                                 aGATGACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGATTCGGTGACACGCTTTTTTATCCGAATGTTGCCAGCCGAGCGGCGAATTTATTGTATTTTATTATCAAAAATCATCCGTTGACCGATGGCAATAAACGCACGGGATCTTTCCTGTTTTTATGGt                                                             >  1:612487/1‑251 (MQ=255)                                                                                                                                                                                     gACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGGCAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGATTCGGTGACACGCTTTTTTATCCGAATGTTGCCAGCCGAGCGGCGAATTTATTGTATTTTATTATCAAAAATCATCCGTTGACCGATGGCAATAAACGCACGGGATCTTTCCTGTTTTTATGGTAttt                                                         >  1:672193/1‑251 (MQ=255)                                                                                                                                                                                     gACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGATTCGGTGACACGCTTTTTTATCCGAATGTTGCCAGCCGAGCGGCGAATTTATTGTATTTTATTATCAAAAATCATCCGTTGACCGATGGCAATAAACGCACGGGATCTTTCCTGTTTTTATGGTAttt                                                         >  1:832347/1‑251 (MQ=255)                                                                                                                                                                                      aCGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGac                                                                                                                                                                                                                                           >  1:363127/1‑72 (MQ=255)                                                                                                                                                                                      aCGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGATTCGGTGACACGCTTTTTTATCCGAATGTTGCCAGCCGAGCGGCGAATTTATTGTATTTTATTATCAAAAATCATCCGTTGACCGATGGCAATAAACGCACGGGATCTTTCCTGTTTTTATGGTATTTg                                                        >  1:3103/1‑251 (MQ=255)                                                                                                                                                                                         tATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGATTCGGTGACACGCTTTTTTATCCGAATGTTGCCAGCCGAGCGGCGAATTTATTGTATTTTATTATCAAAAATCATCCGTTGACCGATGGCAATAAACGCACGGGATCTTTCCTGTTTTTATGGTATTTGCgg                                                     <  1:698017/251‑1 (MQ=255)                                                                                                                                                                                                    aTTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGATTCGGTGACACGCTTTTTTATCCGAATGTTGCCAGCCGAGCGGCGAATTTATTGTATTTTATTATCAAAAATCATCCGTTGACCGATGGCAATAAACGCACGGGATCTTTCCTGTTTTTATGGTATTTGCGGTTGAATCAaca                                          >  1:249776/1‑251 (MQ=255)                                                                                                                                                                                                     tttCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGATTCGGTGACACGCTTTTTTATCCGAATGTTGCCAGCCGAGCGGCGAATTTAtt                                                                                                                                    <  1:706422/160‑1 (MQ=255)                                                                                                                                                                                                     tttCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGATTCGGTGACACGCTTTTTTATCCGAATGTTGCCAGCCGAGCGGCGAATTTATTGTATTTTATTATCAAAAATCATCCGTTGACCGATGGCAATAAACGCACGGGATCTTTCCTGTTTTTATGGTATTTGCGGTTGAATCAacac                                         <  1:623770/251‑1 (MQ=255)                                                                                                                                                                                                     tttCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGATTCGGTGACACGCTTTTTTATCCGAATGTTGCCAGCCGAGCGGCGAATTTATTGTATTTTATTATCAAAAATCATCCGTTGACCGATGGCAATAAACGCACGGGATCTTTCCTGTTTTTATGGTATTTGCGGTTGAATCAacac                                         <  1:78679/251‑1 (MQ=255)                                                                                                                                                                                                     tttCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGATTCGGTGACACGCTTTTTTATCCGAATGTTGCCAGCCGAGCGGCGAATTTATTGTATTTTATTATCAAAAATCATCCGTTGACCGATGGCAATAAACGCACGGGATCTTTCCTGTTTTTATGGTATTTGCGGTTGAATCAacac                                         <  1:335900/251‑1 (MQ=255)                                                                                                                                                                                                          cAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACaa                                                                                                                                                                                            <  1:898018/99‑1 (MQ=255)                                                                                                                                                                                                            aTTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGATTCGGTGACACGCTTTTTTATCCGAATGTTGACAGCCGAGCGGCGAATTTATTGTATTTTATTATCAAAAATCATCCGTTGACCGATGGCAATAAACGCACGGGATCTTTCCTGTTTTTATGGTATTTGCGGTTGAATCAacac                                         >  1:344599/1‑244 (MQ=255)                                                                                                                                                                                                             ttAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGATTCGGTGACACGCTTTTTTATCCGAATGTTGCCAGCCGAGCGGCGAATTTATTGTATTTTATTATCAAAAATCATCCGTTGACCGATGGCAATAAACGCACGGGATCTTTCCTGTTTTTATGGTATTTGCGGTTGAATCAACACTTATTGGc                                 <  1:810608/251‑1 (MQ=255)                                                                                                                                                                                                                aaaCAGGCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGATTCGGTGACACGCTTTTTTATCCGAATGTTGCCAGCCGAGCGGCGAATTTATTGTATTTTATTATCAAAAATCGTCCGTTGACCGATGGCAATAAACGCACGGGATCTTTCCTGTTTTTATGGTATTTGCGGTTGAATCAACACTTATTGGCAAg                              >  1:456341/1‑251 (MQ=255)                                                                                                                                                                                                                      gCGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGATTCGGTGACACGCTTTTTTATCCGAATGTTGCCAGCCGAGCGGCGAATTTATTGTATTTTATTATCAAAAATCATCCGTTGACCGATgg                                                                                                >  1:406308/1‑179 (MQ=255)                                                                                                                                                                                                                       cGTTAGTGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGATTCGGTGACACGCTTTTTTATCCGAATGTTGCCAGCCGAGCGGCGAATTTATTGTATTTTATTATCAAAAATCATCCGTTGACCGATGGCAATAAACGCACGGGATCTTTCCTGTTTTTATGGTATTTGCGGTTGAATCAACACTTATTGGCAAGGCCGGTa                       >  1:201599/1‑251 (MQ=255)                                                                                                                                                                                                                             tGGAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGATTCGGTGACACGCTTTTTTATCCGAATGTTGCCAGCCGAGCGGCGAATTTATTGTATTTTATTATCAAAAATCATCCGTTGACCGATGGCAATAAACGCACGGGATCTTTCCTGTTTTTATGGTATTTGCGGTTGAATCAACACTTATTGGCAAGGCCGGTAGAAc                   <  1:587726/249‑1 (MQ=255)                                                                                                                                                                                                                               gAAAAAGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGATTCGGTGACACGCTTTTTTATCCGAATGTTGCCAGCCGAGCGGCGAATTTATTGTATTTTATTATCAAAAATCATCCGTTGACCGATGACAATAAACGCACGGGATCTTTCCTGTTTTTATGGTATTTGCGGTTGAATCAACACTTa                                      >  1:451803/1‑228 (MQ=255)                                                                                                                                                                                                                                aaaaaGGGGAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGATTCGGTGACACGCTTTTTTATCCGAATGTTGCCAGCCGAGCGGCGAATTTATTGTATTTTATTATCAAAAATCATCCGTTGACCGATGGCAATAAACGCACGGGATCTTTCCTGTTTTTATGGTATTTGCGGTTGAATCAACACTTATTGGCAAGGCCGGTAGAACAATTg              <  1:596048/251‑1 (MQ=255)                                                                                                                                                                                                                                     ggggAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGATTCGGTGACACGCTTTTTTATCCGAATGTTGCCAGCCGAGCGGCGAATTTATTGTATTTTATTATCAAAAATCATCCGTTGACCGATGGCAATAAACGCACGGGATCTTTCCTGTTTTTATGGTATTTGCGGTTGAATCAACACTTATTGGCAAGGCCGGTAGAACAATTGATTaa         >  1:901150/1‑251 (MQ=255)                                                                                                                                                                                                                                      gggAAGCGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGcc                                                                                                                                                                                                              >  1:547567/1‑53 (MQ=255)                                                                                                                                                                                                                                            cGACAGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTGCCACGATTGAACAAGGATTCGGTGACACGCTTTTTTATCCGAATGTTGCCAGCCGAGCGGCGAATTTATTGTATTTTATTATCAAAAATCATCCGTTGACCGATGGCAATAAACGCACGGGATCTTTCCTGTTTTTATGGTATTTGCGGTTGAATCAACACTTATTGGCAAGGCCGGTAGAACAATTGATTAATGACAAt  >  1:879739/1‑251 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                       GTGATAGGTTTGCTTGGGCAGACCTTGAGTAATCAATCTTTGTTATCTGACGAAGGCAAAGCCGTATTGTCTGTTGTGCAGGATTATGCTAGGAGTTGGAGTTTGTTACAGGCTTACGATGAGCAAAGTCTAACGGCTAATCAGCATAAGCAAAATAATATGGTTCTGTTGGTGTTAGATGACGTATTACAGGCAATTTCTCAATTAAAACAGGCGTTAGTGGAAAAAGGGGAAGCGACGGCGTTGTTTGGACAACAACGAAATTCCGGTTTAGCCTCTGCCATTACCACGATTGAACAAGGATTCGGTGACACGCTTTTTTATCCGAATGTTGCCAGCCGAGCGGCGAATTTATTGTATTTTATTATCAAAAATCATCCGTTGACCGATGGCAATAAACGCACGGGATCTTTCCTGTTTTTATGGTATTTGCGGTTGAATCAACACTTATTGGCAAGGCCGGTAGAACAATTGATTAATGACAAT  >  1/1178530‑1179015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑