Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,176,999	G→T	intergenic (+17419/+243938)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,176,999	0	G	T	100.0%	179.9 / NA	67	intergenic (+17419/+243938)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (36/31);  total (36/31)

ATCAAAGCGAAAATCGAGCAGGAAGAAGGCAATTTCAATTTTGCCGTGTTGGAAAGTGCGGTGGAAAATGCGCAATATTTGGATATTCGTCAAATCGCCGAGCAGACGGAGAAAGAGGTGGTTTCCGTCGATACCATTTCCGTGCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCGTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGTATTGCGAACGCGGCGTGATGAGCAAATTGCAGGCGCTGTATTTGAAAGAGAGTGGATTTAATAATGTGAAGGTGTTCAAAAAGATACTTTCCTAATGCCTTCCTCGAATCAAAAGTGCGGTGGGTTTTCACGATGAAAAAACGTTGGGAAAACCGACCGCACTTTTTTATTTCAATCATTAC  >  1/1176760‑1177239                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                 aTCAAAGCGAAAATCGAGCAGGAAGAAGGCAATTTCAATTTTGCCGTGTTGGAAAGTGCGGTGGAAAATGCGCAATATTTGGATATTCGTCAAATCGCCGAGCAGACGGAGAAAGAGGTGGTTTCCGTCGATACCATTTCCGTCCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACaaa                                                                                                                                                                                                                                         >  1:896547/1‑249 (MQ=255)    aaaGCGAAAATCGAGCAGGAAGAAGGCAATTTCAATTTTGCCGTGTTGGAAAGTGCGGTGGAAAATGCGCAATATTTGGATATTCGTCAAATCGCCGAGCAGACGGAGAAAGAGGTGGTTTCCGTCGATACCATTTCCGTGCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTc                                                                                                                                                                                                                                    <  1:162091/251‑1 (MQ=255)          aaaaTCGAGCAGGAAGAAGGCAATTTCAATTTTGCCGTGTTGGAAAGTGCGGTGGAAAATGCGCAATATTTGGATATTCGTCAAATCGCCGAGCAGACGGAGAAAGAGGTGGTTTCCGTCGATACCATTTCCGTGCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCcaatc                                                                                                                                                                                                                               <  1:818451/250‑1 (MQ=255)                   cAGGAAGAAGGCAATTTCAATTTTGCCGTGTTGGAAAGTGCGGTGGAAAATGCGCAATATTTGGATATTCGTCAAATCGCCGAGCAGACGGAGAAAGAGGTGGTTTCCGTCGATACCATTTCCGTGCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGcc                                                                                                                                                                                                                       <  1:229202/249‑1 (MQ=255)                          aagGCAATTTCAATTTTGCCGTGTTGGAAAGTGCGGTGGAAAATGCGCAATATTTGGATATTCGTCAAATCGCCGAGCAGACGGAGAAAGAGGTGGTTTCCGTCGATACCATTTCCGTGCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGAt                                                                                                                                                                                                              <  1:120360/251‑1 (MQ=255)                                     aatattGCCGTGTTGGATAGTGCGGTGGAAAATGCGCAATATTTGGATATTCGTCAAATCGCCGATCAGACGGAGAAATCTGTGGTTTCCGTCTATACCATGTCCTTGCTGGGCATCAATGTCTTGATGTTCGACATCCGCATCCCGGAGGTCCTCGCCGAAAAACCGCTACATATTGACAAGCCTACGATGATTTTTTTGCCTTTTTTCACTCTGTCCAATCACTTTGCCGAACTGGATCAAAGCaaaaa                                                                                                                                                                                                   <  1:725796/247‑1 (MQ=255)                                      atttTGCCGTGTTGGAAAGTGCGGTGGAAAATGCGCAATATTTGGATATTCGTCAAATCGCCGAGCAGACGGAGAAAGAGGTGGTTTCCGTCGATACCATTTCCGTGCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCaaaaa                                                                                                                                                                                                   >  1:202144/1‑250 (MQ=255)                                              gtgtTGGAAAGTGCGGTGGAAAATGCGCAATATTTGGATATTCGTCAAATCGCCGAGCAGACGGAGAAAGAGGTGGTTTCCGTCGATACCATTTCCGTGCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAAc                                                                                                                                                                                                                                        >  1:344842/1‑205 (MQ=255)                                              gtgtTGGAAAGTGCGGTGGAAAATGCGCAATATTTGGATATTCGTCAAATCGCCGAGCAGACGGAGAAAGAGGTGGTTTCCGTCGATACCATTTCCGTGCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTAtgtg                                                                                                                                                                                            >  1:753549/1‑249 (MQ=255)                                              gtgtTGGAAAGTGCGGTGGAAAATGCGCAATATTTGGATATTCGTCAAATCGCCGAGCAGACGGAGAAAGAGGTGGTTTCCGTCGATACCATTTCCGTGCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTAtgtg                                                                                                                                                                                            >  1:644009/1‑249 (MQ=255)                                              gtgtTGGAAAGTGCGGTGGAAAATGCGCAATATTTGGATATTCGTCAAATCGCCGAGCAGACGGAGAAAGAGGTGGTTTCCGTCGATACCATTTCCGTGCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGc                                                                                                                                                                                           <  1:353302/250‑1 (MQ=255)                                                gtTGGAAAGTGCGGTGGAAAATGCGCAATATTTGGATATTCGTCAAATCGCCGAGCAGACGGAGAAAGAGGTGGTTTCCGTCGATACCATTTCCGTGCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTAtgtg                                                                                                                                                                                            >  1:171972/1‑247 (MQ=255)                                                     aaaGTGCGGTGGAAAATGCGCAATATTTGGATATTCGTCAAATCGCCGAGCAGACGGAGAAAGAGGTGGTTTCCGTCGATACCATTTCCGTGCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAATCCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGTATTg                                                                                                                                                                                    <  1:567235/250‑1 (MQ=255)                                                        gTGCGGTGGAAAATGCGCAATATTTGGATATTCGTCAAATCGCCGAGCAGACGGAGAAAGAGGTGGTTTCCGTCGATACCATTTCCGTGCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGTATTGCGaa                                                                                                                                                                                >  1:740305/1‑251 (MQ=255)                                                         tGCGGTGGAAAATGCGCAATATTTGGATATTCGTCAAATCGCCGAGCAGACGGAGAAAGAGGTGGTTTCCGTCGATTCCATTTCCGTGCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGTATTGCGa                                                                                                                                                                                 <  1:610928/249‑1 (MQ=255)                                                                 aaaaTGCGCAATATTTGGATATTCGTCAAATCGCCGAGCAGACGGAGAAAGAGGTGGTTTCCGTCGATACCATTTCCGTGCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATc                                                                                                                                                                                                             >  1:81426/1‑213 (MQ=255)                                                                      gcgcAATATTTGGATATTCGTCAAATCGCCGAGCAGACGGAGAAAGAGGTGGTTTCCGTCGATACCATTTCCGTGCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGTATTGCGAACGCGGCGTGATGAg                                                                                                                                                                  >  1:281526/1‑251 (MQ=255)                                                                         cAATATTTGGATATTCGTCAAATCGCCGAGCAGACGGAGAAAGAGGTGGTTTCCGTCGATACCATTTCCGTGCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGc                                                                                                                                                                                                        >  1:769852/1‑210 (MQ=255)                                                                           atatTTGGATATTCGTCAAATCGCCGAGCAGACGGAGAAAGAGGTGGTTTCCGTCGATACCATTTCCGTGCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGTAt                                                                                                                                                                                      >  1:781983/1‑226 (MQ=255)                                                                           atatTTGGATATTCGTCAAATCGCCGAGCAGACGGAGAAAGAGGTGGTTTCCGTCGATACCATTTCCGTGCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGTATTGCGa                                                                                                                                                                                 <  1:259891/231‑1 (MQ=255)                                                                           atatTTGGATATTCGTCAAATCGCCGAGCAGACGGAGAAAGAGGTGGTTTCCGTCGATACCATTTCCGTGCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGTATTGCGAACGCGGCGTGATGAGCAAAt                                                                                                                                                             >  1:803381/1‑251 (MQ=255)                                                                           atatTTGGATATTCGTCAAATCGCCGAGCAGACGGAGAAAGAGGTGGTTTCCGTCGATACCATTTCCGTGCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGTATTGCGAACGCGGCGTGATGAGCAAAt                                                                                                                                                             >  1:783636/1‑251 (MQ=255)                                                                           atatTTGGATATTCGTCAAATCGCCGAGCAGACGGAGAAAGAGGTGGTTTCCGTCGATACCATTTCCGTGCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGTATTGCGAACGCGGCGTGATGAGCAAAt                                                                                                                                                             >  1:605959/1‑251 (MQ=255)                                                                           atatTTGGATATTCGTCAAATCGCCGAGCAGACGGAGAAAGAGGTGGTTTCCGTCGATACCATTTCCGTGCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGTATTGCGAACGCGGCGTGATGAGCAAAt                                                                                                                                                             >  1:297947/1‑251 (MQ=255)                                                                           atatTTGGATATTCGTCAAATCGCCGAGCAGACGGAGAAAGAGGTGGTTTCCGTCGATACCATTTCCGTGCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGTATTGCGAACGCGGCGTGATGAGCAAAt                                                                                                                                                             >  1:297512/1‑251 (MQ=255)                                                                           atatTTGGATATTCGTCAAATCGCCGAGCAGACGGAGAAAGAGGCGGTTTCCGTCGATACCATTTCCGTGCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTCACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGt                                                                                                                                                                                        >  1:443633/1‑224 (MQ=255)                                                                             atTTGGATATTCGTCAAATCGCCGAGCAGACGGAGAAAGAGGTGGTTTCCGTCGATACCATTTCCGTGCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGTATTGCGAACGCGGCGTGATGAGCAAATTg                                                                                                                                                           >  1:382880/1‑251 (MQ=255)                                                                             atTTGGATATTCGTCAAATCGCCGAGCAGACGGAGAAAGAGGTGGTTTCCGTCGATACCATTTCCGTGCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGTATTGCGAACGCGGCGTGATGAGCAAATTg                                                                                                                                                           >  1:412205/1‑251 (MQ=255)                                                                                              aTCGCCGAGCAGACGGAGAAAGAGGTGGTTTCCGTCGATACCATTTCCGTGCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGTATTGCGAACGCGGCGTGATGAGCAAATTGCAGGCGCTGTATTTGaa                                                                                                                                          <  1:738295/251‑1 (MQ=255)                                                                                              aTCGCCGAGCAGACGGAGAAAGAGGTGGTTTCCGTCGATACCATTTCCGTGCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGTATTGCGAACGCGGCGTGATGAGCAAATTGCAGGCGCTGTATTTGaa                                                                                                                                          <  1:287719/251‑1 (MQ=255)                                                                                              aTCGCCGAGCAGACGGAGAAAGAGGTGGTTTCCGTCGATACCATTTCCGTGCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGTATTGCGAACGCGGCGTGATGAGCAAATTGCAGGCGCTGTATTTGaa                                                                                                                                          <  1:411965/251‑1 (MQ=255)                                                                                              aTCGCCGAGCAGACGGAGAAAGAGGTGGTTTCCGTCGATACCATTTCCGTGCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGTATTGCGAACGCGGCGTGATGAGCAAATTGCAGGCGCTGTATTTGaa                                                                                                                                          <  1:340778/251‑1 (MQ=255)                                                                                              aTCGCCGAGCAGACGGAGAAAGAGGTGGTTTCCGTCGATACCATTTCCGTGCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGTATTGCGAACGCGGCGTGATGAGCAAATTACAGGCGCTGTATTTGaa                                                                                                                                          <  1:508112/251‑1 (MQ=255)                                                                                                             gagaAAGAGGTGGTTTCCGTCGATACCATTTCCGTGCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCcaatcaa                                                                                                                                                                                                                             <  1:848277/153‑1 (MQ=255)                                                                                                             gagaAAGAGGTGGTTTCCGTCGATACCATTTCCGTGCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGTATTGCGAACGCGGCGTGATGAGCAAATTGCAGGCGCTGTATTTGAAAGAGAGTGGATTtaa                                                                                                                           >  1:584413/1‑251 (MQ=255)                                                                                                              agaAAGAGGTGGTTTCCGTCGATACCATTTCCGTGCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGTATTGCGAACGCGGCGTGATGAGCAAATTGCAGGCGCTGTATTTGAAAGAGAGTGGATTtaat                                                                                                                          >  1:173945/1‑251 (MQ=255)                                                                                                                aaaGAGGTGGTTTCCGTCGATACCATTTCCGTGCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGTATTGCGAACGCGGCGTGATGAGCAAATTGCAGGCGCTGTATTTGAAAGAGAGTGGATTtaat                                                                                                                          <  1:235821/249‑1 (MQ=255)                                                                                                                     ggtggtTTCCGTCGATACCATTTCCGTGCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGTATTGCGAACGCGGCGTGATGAGCAAATTGCAGGCGCTGTATTTGAAAGAGAGTGGATTTAATAATGTGa                                                                                                                   >  1:54920/1‑251 (MQ=255)                                                                                                                         gtTTCCGTCGATACCATTTCCGTGCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGTATTGCGAACGCGGCGTGATGAGCAAATTGCAGGCGCTGTATTTGAAAGAGAGTGGATTTAATAATGTGAAgg                                                                                                                <  1:785366/250‑1 (MQ=255)                                                                                                                         gtTTCCGTCGATACCATTTCCGTGCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGTATTGCGAACGCGGCGTGATGAGCAAATTGCAGGCGCTGTATTTGAAAGAGAGTGGATTTAATAATGTGAAgg                                                                                                                <  1:589423/250‑1 (MQ=255)                                                                                                                         gtTTCCGTCGATACCATTTCCGTGCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGTATTGCGAACGCGGCGTGATGAGCAAATTGCAGGCGCTGTATTTGAAAGAGAGTGGATTTAATAATGTGAAgg                                                                                                                <  1:135388/250‑1 (MQ=255)                                                                                                                              cGTCGATACCATTTCCGTGCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGTATTGCGAACGCGGCGTGATGAGCAAATTGCAGGCGCTGTATTTGAAAGAGAGTGGATTTAATAATGTGAAGGTGTTCa                                                                                                          >  1:298600/1‑251 (MQ=255)                                                                                                                               gTCGATACCATTTCCGTGCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGTATTGCGAACGCGGCGTGATGAGCAAATTGCAGGCGCTGTATTTGAAagag                                                                                                                                      >  1:801659/1‑222 (MQ=255)                                                                                                                               gTCGATACCATTTCCGTGCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCCGTATTGCGAACGCGGCGTGATGAGCAAATTGCAGGCGCTGTATTTGAAAGAGAGTGGATTTAATAATGTGAAGGTGTTCaa                                                                                                         >  1:83716/1‑251 (MQ=255)                                                                                                                                    tACCATTTCCGTGCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGTATTGCGAACGCGGCGTGATGAGCAAATTGCAGGCGCTGTATTTGaa                                                                                                                                          <  1:162919/213‑1 (MQ=255)                                                                                                                                    tACCATTTCCGTGCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGTATTGCGAACGCGGCGTGATGAGCAAATTGCAGGCGCTGTATTTGAAag                                                                                                                                        >  1:630237/1‑215 (MQ=255)                                                                                                                                      ccATTTCCGTGCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGTATTGCGAACGCGGCGTGATGAGCAAATTGCAGGCGCTGTATTTGAAAGAGAGTGGATTTAATAATGTGAAGGTGTTCAAAAAGATa                                                                                                  >  1:113446/1‑251 (MQ=255)                                                                                                                                         tttCCGTGCTGGGCATCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGTATTGCGAACGCGGCGTGATGAGc                                                                                                                                                                 <  1:454250/185‑1 (MQ=255)                                                                                                                                         tttCCGTGCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGc                                                                                                                                                                                           >  1:635090/1‑159 (MQ=255)                                                                                                                                           tCCGTGCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGTAt                                                                                                                                                                                      <  1:413759/162‑1 (MQ=255)                                                                                                                                                gCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGTATTGCGAACGCGGCGTGATGAGCAAATTGCAGGCGCTGTATTTGAAAGAGAGTGGATTTAATAATGTGAAgg                                                                                                                >  1:577443/1‑227 (MQ=255)                                                                                                                                                 cTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAAc                                                                                                                                                                                                  >  1:339502/1‑144 (MQ=255)                                                                                                                                                  tGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGTATTGCGAACGCGGCGTGATGAGCAAATTGCAGGCGCTGTATTTGAAAGAGAGTGGATTTAATAATGTGAAGGTGTTCAAAAAGATACTTTCCTAATGc                                                                                      <  1:46286/251‑1 (MQ=255)                                                                                                                                                           aTGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGTATTGCGAACGCGGCGTGATGAGCAAATTGCAGGCGCTGTATTTGAAAGAGAGTGGATTTAATAATGTGAAGGTGTTCAAAAAGATACTTTCCTAATGCCTTCCTCGa                                                                             >  1:480819/1‑251 (MQ=255)                                                                                                                                                               cGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGTATTGCGAACGCGGCGTGATGAGCAAATTGCAGGCGCTGTATTTGAAAGAGAGTGGATTtaat                                                                                                                          <  1:836790/202‑1 (MQ=255)                                                                                                                                                                gTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGTATTGCGAACGCGGCGTGATGAGCAAATTGCAGGCGCTGTATTTGAAAGAGAGTGGATTTAATAAtg                                                                                                                      <  1:505524/205‑1 (MQ=255)                                                                                                                                                                      atcGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGTATTGCGAACGCGGCGTGATGAGCAAATTGCAGGCGCTGTATTTGAAAGAGAGTGGATTtaat                                                                                                                          >  1:698663/1‑195 (MQ=255)                                                                                                                                                                                   gCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGTATTGCGAACGCGGCGTGATGAGCAAATTGCAGGCGCTGTATTTGAAAGAGAGTGGATTTAATAATGTGAAGGTGTTCAAAAAGATAc                                                                                                 <  1:691737/207‑1 (MQ=255)                                                                                                                                                                                   gCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGTATTGCGAACGCGGCGTGATGAGCAAATTGCAGGCGCTGTATTTGAAAGAGAGTGGATTTAATAATGTGAAGGTGTTCAAAAAGATACTTTc                                                                                             <  1:562469/211‑1 (MQ=255)                                                                                                                                                                                   gCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGTATTGCGAACGCGGCGTGATGAGCAAATTGCAGGCGCTGTATTTGAAAGAGAGTGGATTTAATAATGTGAAGGTGTTCAAAAAGATACTTTCCTAATGCCTTCCTCGAATCAAAAGTGCGGTGGGTTTTCa                                                      >  1:715807/1‑250 (MQ=255)                                                                                                                                                                                       ggaggaAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGTATTGCGAACGCGGCGTGATGAGCAAATTGCAGgcg                                                                                                                                                     >  1:59699/1‑151 (MQ=255)                                                                                                                                                                                                     aaaaaCCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGTATTGCGAACGCGGCGTGATGAGCAAATTGCAGGCGCTGTATTTGAAAGAGAGTGGATTTAATAATGTGAAGGTGTTCAAAAAGATACTTTCCTAATGCCTTCCTCGAATCAAAAGTGCGGTGGGTTTTCACGATGAAAAAACGTTGGGa                                   <  1:750899/251‑1 (MQ=255)                                                                                                                                                                                                            gCTACATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGTATTGCGAACGCGGCGTGATGAGCAAATTGCAGGCGCTGTATTTGaa                                                                                                                                          <  1:295531/141‑1 (MQ=255)                                                                                                                                                                                                               aCATATTGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGTATTGCGAACGCGGCGTGATGAGCAAATTGCAGGCGCTGTATTTGAAAGAGAGTGGATTTAATAATGTGAAGGTGTTCAAAAAGATACTTTCCTAATGCCTTCCTCGAATCAAAAGTGCGGTGGGTTTTCACGATGAAAAAACGTTGGGAAAaccgaccg                         >  1:245251/1‑251 (MQ=255)                                                                                                                                                                                                                    ttGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGTATTGCGAACGCGGCGTGATGAGCAAATTGCAGGCGCTGTATTTGAAAGAGAGTGGATTtaat                                                                                                                          <  1:479199/149‑1 (MQ=255)                                                                                                                                                                                                                    ttGAAAAGCAAACGATGATTTTGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGTATTGCGAACGCGGCGTGATGAGCAAATTGCAGGCGCTGTATTTGAAAGAGAGTGGATTTAATAATGTGAAGGTGTTCAAAAAGATACTTTCCTAATGCCTTCCTCGAATCAAAAGTGCGGTGGGTTTTCACGATGAAAAAACGTTGGGAAAACCGACCGCACtt                    >  1:162231/1‑251 (MQ=255)                                                                                                                                                                                                                                      ttttGTTGCCTTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGTATTGCGAACGCGGCGTGATGAGCAAATTGCAGGCGCTGTATTTGAAAGAGAGTGGATTTAATAATGTGAAGGTGTTCAAAAAGATACTTTCCTAATGCCTTCCTCGAATCAAAAGTGCGGTGGGTTTTCACGATGAAAAAACGTTGGGAAAACCGACCGCACTTTTTTATTTCAATCATTAc  <  1:689831/251‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                 ATCAAAGCGAAAATCGAGCAGGAAGAAGGCAATTTCAATTTTGCCGTGTTGGAAAGTGCGGTGGAAAATGCGCAATATTTGGATATTCGTCAAATCGCCGAGCAGACGGAGAAAGAGGTGGTTTCCGTCGATACCATTTCCGTGCTGGGCACCAATGACGTGATCATCGACATCCGCAGCCCGGAGGAAATCGACGAAAAACCGCTACATATTGAAAAGCAAACGATGATTTTGTTGCCGTTTTACAAACTGTCCAATCAATTTGCCGAACTGGATCAAAGCAAAAACTATGTGCTGTATTGCGAACGCGGCGTGATGAGCAAATTGCAGGCGCTGTATTTGAAAGAGAGTGGATTTAATAATGTGAAGGTGTTCAAAAAGATACTTTCCTAATGCCTTCCTCGAATCAAAAGTGCGGTGGGTTTTCACGATGAAAAAACGTTGGGAAAACCGACCGCACTTTTTTATTTCAATCATTAC  >  1/1176760‑1177239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑