Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,175,581	A→C	intergenic (+16001/+245356)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,175,581	0	A	C	100.0%	27.2 / NA	12	intergenic (+16001/+245356)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (2/10);  total (2/10)

AGCCCCATCAGGCAAAGTGCGGTTATAATTTTTTTTGTTTTTGACATGGAATCACTCCGATGTTTGAGCGTTTTAATAACTAAAAGGAATTTATAACTACAACGAGTTAGTGTTGATTCTAGTGTATCTAAATATAAATACTTTGATTTCAGTCAAGTAAAATAAATTAATTCCTTTATTTCCTTATAAATCAGTAAATTAATTTCATTTCTGTGATAACAATTACGATATAATCAATAGTTATATATATTTTTATATTAAGAGGGTTTAAAAGGAGTTTGAGCCAATGGTAATTTTTGTGCTATCTGCTAAAATACGCCCCATTTTTCTATGGGTTAAAAAGTGAATTAACATGAAATTTATCGTCAAATTATTTCCTGAAATTATGATTAAAAGCGAATCCGTGCGGAAACGGTTCGTGAAAATTCTGACCGGCAATATTCGTAACGTAT  >  1/1175343‑1175794                                                                                                                                                                                                                                               |                                                                                                                                                                                                                      aGCCCCATCAGGCAAAGTGCGGTTATAATTTTTTTTGTTTTTGACATGGAATCACTCCGATGTTTGAGCGTTTTAATAACTAAAAGGAATTTATAACTACAACGAGTTAGTGTTGATTCTAGTGTATCTAAATATAAATACTTTGATTTCAGTCAAGTAAAATAAATTAATTCCTTTATTTCCTTATAAATCAGTAAATTAATTTCATTTCTGTGATAACAATTACGATATAATCAATCGTtatatat                                                                                                                                                                                                              <  1:894268/248‑1 (MQ=255)                       ttATAATTTTTTTTGTTTTTGACATGGAATCACTCCGATGTTTGAGCGTTTTAATAACTAAAAGGAATTTATAACTACAACGAGTTAGTGTTGATTCTAGTGTATCTAAATATAAATACTTTGATTTCAGTCAAGTAAAATAAATTAATTCCTTTATTTCCTTATAAATCAGTAAATTAATTTCATTTCTGTGATAACAATTACGATATAATCAATCGTTATATATATTTTTATATTAAGAGGGTTTaaa                                                                                                                                                                                      <  1:323273/250‑1 (MQ=255)                                             cATGGAATCACTCCGATGTTTGAGCGTTTTAATAACTAAAAGGAATTTATAACTACAACGAGTTAGTGTTGATTCTAGTGTATCTAAATATAAATACTTTGATTTCAGTCAAGTAAAATAAATTAATTCCTTTATTTCCTTATAAATCAGTAAATTAATTTCATTTCTGTGATAACAATTACGATATAATCAATCGTTATATATATTTTTATATTAAGAGGGTTTAAAAGGAGTTTGAGCCAATGGTAAtt                                                                                                                                                               >  1:884860/1‑251 (MQ=255)                                                               tttGAGCGTTTTAATAACTAAAAGGAATTTATAACTACAACGAGTTAGTGTTGATTCTAGTGTATCTAAATATAAATACTTTGATTTCAGTCAAGTAAAATAAATTAATTCCTTTATTTCCTTATAAATCAGTAAATTAATTTCATTTCTGTGATAACAATTACGATATAATCAATCGTTATATATATTTTTATATTAAGAGGGTTTAAAAGGAGTTTGAGCCAATGGTAATTTTTGTGCTATCTGCTaa                                                                                                                                              <  1:242020/250‑1 (MQ=255)                                                                   aGCGTTTTAATAACTAAAAGGAATTTATAACTACAACGAGTTAGTGTTGATTCTAGTGTATCTAAATATAAATACTTTGATTTCAGTCAAGTAAAATAAATTAATTCCTTTATTTCCTTATAAATCAGTAAATTAATTTCATTTCTGTGATAACAATTACGATATAATCAATCGTTATATATATTTTTATATTAAGAGGGTTTAAAAGGAGTTTGAGCCAATGGTAATTTTTGTGCTATCTGCTAAAATa                                                                                                                                          <  1:51444/250‑1 (MQ=255)                                                                                                    cAACGAGTTAGTGTTGATTCTAGTGTATCTAAATATAAATACTTTGATTTCAGTCAAGTAAAATAAATTAATTCCTTTATTTCCTTATAAATCAGTAAATTAATTTCATTTCTGTGATAACAATTACGATATAATCAATCGTTATATATATTTTTATATTAAGAGGGTTTAAAAGGAGTTTGAGCCAATGGTAATTTTTGTGCTATCTGCTAAAATACGCCCCATTTTTCTATGGGTTAAAAAGTGAATTa                                                                                                        <  1:157798/251‑1 (MQ=255)                                                                                                                               tCTAAATATAAATACTTTGATTTCAGTCAAGTAAAATAAATTAATTCCTTTATTTCCTTATAAATCAGTAAATTAATTTCATTTCTGTGATAACAATTACGATATAATCAATCGTTATATATATTTTTATATTAAGAGGGTTTAAAAGGAGTTTGAGCCAATGGTAATTTTTGTGCTATCTGCTAAAATACGCCCCATTTTTCTATGGGTTaaaa                                                                                                                 <  1:387296/215‑1 (MQ=255)                                                                                                                                    atataaataCTTTGATTTCAGTCAAGTAAAATAAATTAATTCCTTTATTTCCTTATAAATCAGTAAATTAATTTCATTTCTGTGATAACAATTACGATATAATCAATCGTTATATATATTTTTATATTAAGAGGGTTTAAAAGGAGTTTGAGCCAATGGTAATTTTTGTGCTATCTGCTAAAATACGCCCCATTTTTCTATGGGTTAAAAAGTGAAt                                                                                                          <  1:94507/217‑1 (MQ=255)                                                                                                                                     tataaataCTTTGATTTCAGTCAAGTAAAATAAATTAATTCCTTTATTTCCTTATAAATCAGTAAATTAATTTCATTTCTGTGATAACAATTACGATATAATCAATCGTtatatat                                                                                                                                                                                                              >  1:229078/1‑116 (MQ=255)                                                                                                                                                                                                          atttcatttcTGTGATAACAATTACGATATAATCAATCGTTATATATATTTTTATATTAAGAGGGTTTAAAAGGAGTTTGAGCCAATGGTAATTTTTGTGCTATCTGCTAAAATACGCCCCATTTTTCTATGGGTTAAAAAGTGAATTAACATGAAATTTATCGTCAAATTATTTCCTGAAATTATGATTAAAAGCGAATCCGTGCGGAAACGGTTCGTGAAAATTCTGACCGGCAATATTCGTAACGTAt  <  1:523327/251‑1 (MQ=255)                                                                                                                                                                                                                                  cGATATAATCAATCGTTATATATATTTTTATATTAAGAGGGTTTAAAAGGAGTTTGAGCCAATGGTAATTTTTGTGCTATCTGCTAAAATACGCCCCATTTTTCTATGGGTTAAAAAGTGAATTAACATGAAATTTATCGTCAAATTATTTCCTGAAATTATGATTAAAAGCGAATCCGTGCGGAAACGGTTCGTGAAAATTCTGACCGGCAATATTc           <  1:834662/218‑1 (MQ=255)                                                                                                                                                                                                                                      ataATCAATCGTTATATATATTTTTATATTAAGAGGGTTTAAAAGGAGTTTGAGCCAATGGTAATTTTTGTGCTATCTGCTAAAATACGCCCCATTTTTCTATGGGTTAAAAAGTGAATTAACa                                                                                                     <  1:356477/124‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                      AGCCCCATCAGGCAAAGTGCGGTTATAATTTTTTTTGTTTTTGACATGGAATCACTCCGATGTTTGAGCGTTTTAATAACTAAAAGGAATTTATAACTACAACGAGTTAGTGTTGATTCTAGTGTATCTAAATATAAATACTTTGATTTCAGTCAAGTAAAATAAATTAATTCCTTTATTTCCTTATAAATCAGTAAATTAATTTCATTTCTGTGATAACAATTACGATATAATCAATAGTTATATATATTTTTATATTAAGAGGGTTTAAAAGGAGTTTGAGCCAATGGTAATTTTTGTGCTATCTGCTAAAATACGCCCCATTTTTCTATGGGTTAAAAAGTGAATTAACATGAAATTTATCGTCAAATTATTTCCTGAAATTATGATTAAAAGCGAATCCGTGCGGAAACGGTTCGTGAAAATTCTGACCGGCAATATTCGTAACGTAT  >  1/1175343‑1175794

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑