Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,175,289	G→A	intergenic (+15709/+245648)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,175,289	0	G	A	100.0%	190.9 / NA	69	intergenic (+15709/+245648)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/44);  total (25/44)

TTCCACACGCGCTGCGCCATTTCCGCACGGTGTTCTTCGTATTTTTCTTTGCTGTCCAATTTGCCCTGCATTTCGTACCAAATATCTTTCAACGCAATGAATTTGGCTTTGACATAATGCCAGCCTTCTTGCGGTACGTGACAATCCGCACATTCGGCGCGGATGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCGGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCACCCAGCCCCATCAGGCAAAGTGCGGTTATAATTTTTTTTGTTTTTGACATGGAATCACTCCGATGTTTGAGCGTTTTAATAACTAAAAGGAATTTATAACTACAACGAGTTAGTGTTGATTCTAGTGTATCTAAATATAAATACTTTGATTTCAGTCAAGTAAAATAAATTAATTCCTTTATTTCCTTATAAATC  >  1/1175043‑1175534                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      ttCCACACGCGCTGCGCCATTTCCGCACGGTGTTCTTCGTATTTTTCTTTGCTGTCCAATTTGCCCTGCATTTCGTACCAAATATCTTTCAACGCAATGAATTTGGCTTTGACATAATGCCAGCCTTCTTGCGGTACGTGACAATCCGCACATTCGGCGCGGATGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGt                                                                                                                                                                                                                                                   >  1:469577/1‑251 (MQ=255) ttCCACACGCGCTGCGCCATTTCCGCACGGTGTTCTTCGTATTTTTCTTTGCTGTCCAATTTGCCCTGCATTTCGTACCAAATATCTTTCAACGCAATGAATTTGGCTTTGACATAATGCCAGCCTTCTTGCGGTACGTGACAATCCGCACATTCGGCGCGGATGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGt                                                                                                                                                                                                                                                   >  1:450538/1‑251 (MQ=255)                    tttCCGCACGGTGTTCTTCGTATTTTTCTTTGCTGTCCAATTTGCCCTGCATTTCGTACCAAATATCTTTCAACGCAATGAATTTGGCTTTGACATAATGCCAGCCTTCTTGCGGTACGTGACAATCCGCACATTCGGCGCGGATGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACa                                                                                                                                                                                                                                >  1:317431/1‑251 (MQ=255)                     ttCCGCACGGTGTTCTTCGTATTTTTCTTTGCTGTCCAATTTGCCCTGCATTTCGTACCAAATATCTTTCAACGCAATGAATTTGGCTTTGACATAATGCCAGCCTTCTTGCGGTACGTGACAATCCGCACATTCGGCGCGGATGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACaa                                                                                                                                                                                                                               <  1:735097/251‑1 (MQ=255)                           aCGGTGTTCTTCGTATTTTTCTTTGCTGTCCAATTTGCCCTGCATTTCGTACCAAATATCTTTCAACGCAATGAATTTGGCTTTGACATAATGCCAGCCTTCTTGCGGTACGTGGCAATCCGCACATTCGGCGCGGATGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCg                                                                                                                                                                                                                         <  1:339871/251‑1 (MQ=255)                           aCGGTGTTCTTCGTATTTTTCTTTGCTGTCCAATTTGCCCTGCATTTCGTACCAAATATCTTTCAACGCAATGAATTTGGCTTTGACATAATGCCAGCCTTCTTGCGGTACGTGACAATCCGCACATTCGGCGCGGATGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTg                                                                                                                                                                                                                                       <  1:343464/237‑1 (MQ=255)                              gtgtTCTTCGTATTTTTCTTTGCTGTCCAATTTGCCCTGCATTTCGTACCAAATATCTTTCAACGCAATGAATTTGGCTTTGACATAATGCCAGCCTTCTTGCGGTACGTGACAATCCGCACATTCGGCGCGGATGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGtt                                                                                                                                                                                                                       >  1:490555/1‑250 (MQ=255)                               tgtTCTTCGTATTTTTCTTTGCTGTCCAATTTGCCCTGCATTTCGTACCAAATATCTTTCAACGCAATGAATTTGGCTTTGACATAATGCCAGCCTTCTTGCGGTACGTGACAATCCGCACATTCGGCGCGGATGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTc                                                                                                                                                                                                                      <  1:57869/250‑1 (MQ=255)                                 ttcttcGTATTTTTCTTTGCTGTCCAATTTGCCCTGCATTTCGTACCAAATATCTTTCAACGCAATGAATTTGGCTTTGACATAATGCCAGCCTTCTTGCGGTACGTGACAATCCGCACATTCGGCGCGGATGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTccc                                                                                                                                                                                                                    <  1:315780/250‑1 (MQ=255)                                   cttcGTATTTTTCTTTGCTGTCCAATTTGCCCTGCATTTCGTACCAAATATCTTTCAACGCAATGAATTTGGCTTTGACATAATGCCAGCCTTCTTGCGGTACGTGACAATCCGCACATTCGGCGCGGATGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCcac                                                                                                                                                                                                                 <  1:155412/251‑1 (MQ=255)                                   cttcGTATTTTTCTTTGCTGTCCAATTTGCCCTGCATTTCGTACCAAATATCTTTCAACGCAATGAATTTGGCTTTGACATAATGCCAGCCTTCTTGCGGTACGTGACAATCCGCACATTCGGCGCGGATGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCcac                                                                                                                                                                                                                 <  1:860653/251‑1 (MQ=255)                                      cGTATTTTTCTTTGCTGTCCAATTTGCCCTGCATTTCGTACCAAATATCTTTCAACGCAATGAATTTGGCTTTGACATAATGCCAGCCTTCTTGCGGTACGTGACAATCCGCACATTCGGCGCGGATGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAAc                                                                                                                                                                                                              <  1:152194/251‑1 (MQ=255)                                      cGTATTTTTCTTTGCTGTCCAATTTGCCCTGCATTTCGTACCAAATATCTTTCAACGCAATGAATTTGGCTTTGACATAATGCCAGCCTTCTTGCGGTACGTGACAATCCGCACATTCGGCGCGGATGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAAc                                                                                                                                                                                                              <  1:481919/251‑1 (MQ=255)                                       gTATTTTTCTTTGCTGTCCAATTTGCCCTGCATTTCGTACCAAATATCTTTCAACGCAATGAATTTGGCTTTGACATAATGCCAGCCTTCTTGCGGTACGTGACAATCCGCACATTCGGCGCGGATGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACg                                                                                                                                                                                                             >  1:481145/1‑251 (MQ=255)                                        tATTTTTCTTTGCTGTCCAATTTGCCCTGCATTTCGTACCAAATATCTTTCAACGCAATGAATTTGGCTTTGACATAATGCCAGCCTTCTTGCGGTACGTGACAATCCGCACATTCGGCGCGGATGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGc                                                                                                                                                                                                            <  1:663664/251‑1 (MQ=255)                                            tttCTTTGCTGTCCAATTTGCCCTGCATTTCGTACCAAATATCTTTCAACGCAATGAATTTGGCTTTGACATAATGCCAGCCTTCTTGCGGTACGTGACAATCCGCACATTCGGCGCGGATGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGAc                                                                                                                                                                                                                                 >  1:302346/1‑226 (MQ=255)                                                 ttGCTGTCCAATTTGCCCTGCATTTCGTACCAAATATCTTTCAACGCAATGAATTTGGCTTTGACATAATGCCAGCCTTCTTGCGGTACGTGACAATCCGCACATTCGGCGCGGATGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCAcc                                                                                                                                                                                                   <  1:727230/251‑1 (MQ=255)                                                  tGCTGTCCAATTTGCCCTGCATTTCGTACCAAATATCTTTCAACGCAATGAATTTGGCTTTGACATAATGCCAGCCTTCTTGCGGTACGTGACAATCCGCACATTCGGCGCGGATGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCAccc                                                                                                                                                                                                  <  1:184974/251‑1 (MQ=255)                                                  tGCTGTCCAATTTGCCCTGCATTTCGTACCAAATATCTTTCAACGCAATGAATTTGGCTTTGACATAATGCCAGCCTTCTTGCGGTACGTGACAATCCGCACATTCGGCGCGGATGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCAccc                                                                                                                                                                                                  <  1:229490/251‑1 (MQ=255)                                                   gCTGTCCAATTTGCCCTGCATTTCGTACCAAATATCTTTCAACGCAATGAATTTGGCTTTGACATAATGCCAGCCTTCTTGCGGTACGTGACAATCCGCACATTCGGCGCGGATGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGttt                                                                                                                                                                                                                                           <  1:862349/209‑1 (MQ=255)                                                          aaTTTGCCCTGCATTTCGTACCAAATATCTTTCAACGCAATGAATTTGGCTTTGACATAATGCCAGCCTTCTTGCGGTACGTGACAATCCGCACATTCGGCGCGGATGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCACCCAGCCCCAt                                                                                                                                                                                          >  1:20725/1‑251 (MQ=255)                                                           aTTTGCCCTGCATTTCGTACCAAATATCTTTCAACGCAATGAATTTGGCTTTGACATAATGCCAGCCTTCTTGCGGTACGTGACAATCCGCACATTCGGCGCGGATGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCACCCAGCCCCATc                                                                                                                                                                                         <  1:146473/251‑1 (MQ=255)                                                                cccTGCATTTCGTACCAAATATCTTTCAACGCAATGAATTTGGCTTTGACATAATGCCAGCCTTCTTGCGGTACGTGACAATCCGCACATTCGGCGCGGATGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCACCCAGCCCCATCAGGc                                                                                                                                                                                     <  1:893038/250‑1 (MQ=255)                                                                  cTGCATTTCGTACCAAATATCTTTCAACGCAATGAATTTGGCTTTGACATAATGCCAGCCTTCTTGCGGTACGTGACAATCCGCACATTCGGCGCGGATGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCACCCAGCCCCATCAGGCaaa                                                                                                                                                                                  >  1:774835/1‑251 (MQ=255)                                                                    gCATTTCGTACCAAATATCTTTCAACGCAATGAATTTGGCTTTGACATAATGCCAGCCTTCTTGCGGTACGTGACAATCCGCACATTCGGCGCGGATGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCACCCAGCCCCATCAGGCAAAGt                                                                                                                                                                                >  1:697124/1‑251 (MQ=255)                                                                    gCATTTCGTACCAAATATCTTTCAACGCAATGAATTTGGCTTTGACATAATGCCAGCCTTCTTGCGGCACGTGACAATCCGCACATTCGGCGCGGATGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCACCCAGCCCCATCAGGc                                                                                                                                                                                     <  1:337896/246‑1 (MQ=255)                                                                           gTACCAAATATCTTTCAACGCAATGAATTTGGCTTTGACATAATGCCAGCCTTCTTGCGGTACGTGACAATCCGCACATTCGGCGCGGATGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAAc                                                                                                                                                                                                              >  1:491977/1‑214 (MQ=255)                                                                                  atatCTTTCAACGCAATGAATTTGGCTTTGACATAATGCCAGCCTTCTTGCGGTACGTGACAATCCGCACATTCGGCGCGGATGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCACCCAGCCCCATCAGGCAAAGTGCGGTTATAAtttt                                                                                                                                                                  >  1:236819/1‑251 (MQ=255)                                                                                    atCTTTCAACGCAATGAATTTGGCTTTGACATAATGCCAGCCTTCTTGCGGTACGTGACAATCCGCACATTCGGCGCGGATGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCcac                                                                                                                                                                                                                 >  1:149533/1‑202 (MQ=255)                                                                                     tCTTTCAACGCAATGAATTTGGCTTTGACATAATGCCAGCCTTCTTGCGGTACGTGACAATCCGCACATTCGGCGCGGATGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCACCCAGCCCCATCAGGCAAAGTGCGGTTATAAtt                                                                                                                                                                    >  1:277829/1‑246 (MQ=255)                                                                                       tttCAACGCAATGAATTTGGCTTTGACATAATGCCAGCCTTCTTGCGGTACGTGACAATCCGCACATTCGGCGCGGATGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCACCCAGCCCCATCAGGCAAAGTGCGGTTATAAttttt                                                                                                                                                                 <  1:343293/247‑1 (MQ=255)                                                                                                   gAATTTGGCTTTGACATAATGCCAGCCTTCTTGCGGTACGTGACAATCCGCACATTCGGCGCGGATGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCACCCAGCCCCATCAGGCAAAGTGCGGTTATAATTTTTTTTGTTTTTGACATgg                                                                                                                                                 >  1:771042/1‑251 (MQ=255)                                                                                                               gACATAATGCCAGCCTTCTTGCGGTACGTGACAATCCGCACATTCGGCGCGGATGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCACCCAGCCCCATCAGGCAAAGTGCGGTTATAATTTTTTTTGTTTTTGACATGGAATCACTCCGAt                                                                                                                                     >  1:103437/1‑251 (MQ=255)                                                                                                                aCATAATGCCAGCCTTCTTGCGGTACGTGACAATCCGCACATTCGGCGCGGATGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCAc                                                                                                                                                                                                    <  1:322841/187‑1 (MQ=255)                                                                                                                aCATAATGCCAGCCTTCTTGCGGTACGTGACAATCCGCACATTCGGCGCGGATGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCACCCAGCCCCATCAGGCAAAGTGCGGTTATAATTTTTTTTGTTTTTGACATGGAATCACTCCGATg                                                                                                                                    <  1:203800/251‑1 (MQ=255)                                                                                                                 cATAATGCCAGCCTTCTTGCGGTACGTGACAATCCGCACATTCGGCGCGGATGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCACCCAGCCCCATCAGGCAAAGTGCGGTTATAAt                                                                                                                                                                     <  1:787375/217‑1 (MQ=255)                                                                                                                 cATAATGCCAGCCTTCTTGCGGTACGTGACAATCCGCACATTCGGCGCGGATGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCACCCAGCCCCATCAGGCAAAGTGCGGTTATAATTTTTTTTGTTTTTGACATGGAATCACTCCGATGt                                                                                                                                   <  1:778449/251‑1 (MQ=255)                                                                                                                     aTGCCAGCCTTCTTGCGGTACGTGACAATCCGCACATTCGGCGCGGATGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCACCCAGCCCCATCAGGCAAAGTGCGGTTATAATTTTTTTTGTTTTTGACATGGAATCACTc                                                                                                                                         >  1:594579/1‑241 (MQ=255)                                                                                                                        ccAGCCTTCTTGCGGTACGTGACAATCCGCACATTCGGCGCGGATGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCACCCAGCCCCATCAGGCAAAGTGCGGTTATAATTTTTTTTGTTTTTGACATGGAATCACTCCGATGTTTGAGCg                                                                                                                            <  1:461812/251‑1 (MQ=255)                                                                                                                              ttcttGCGGTACGTGACAATCCGCACATTCGGCGCGGATGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCACCCAGCCCCATCAGGCaa                                                                                                                                                                                   <  1:129527/190‑1 (MQ=255)                                                                                                                              ttcttGCGGTACGTGACAATCCGCACATTCGGCGCGGATGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCACCCAGCCCCATCAGGCAAAGTGCGGTTATAATTTTTTTTGTTTTTGACATGGAATCACTCCGATGTTTGAGCGTTTtaa                                                                                                                      <  1:377191/251‑1 (MQ=255)                                                                                                                                 ttGCGGTACGTGACAATCCGCACATTCGGCGCGGATGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTcccc                                                                                                                                                                                                                   <  1:151162/155‑1 (MQ=255)                                                                                                                                      gTACGTGACAATCCGCACATTCGGCGCGGATGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCACCCAGCCCCATCAGGCAAAGTGCGGTTATAAt                                                                                                                                                                     <  1:175819/196‑1 (MQ=255)                                                                                                                                      gTACGTGACAATCCGCACATTCGGCGCGGATGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCACCCAGCCCCATCAGGCAAAGTGCGGTTATAATTTTTTTTGTTTTTGACATGGAATCACTCCGATGTTTGAGCGTTTTAATAACTaaa                                                                                                              >  1:362887/1‑251 (MQ=255)                                                                                                                                               aaTCCGCACATTCGGCGCGGATGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCACCCAGCCCCATCAGGCAAAGTGCGGTTATAATTTTTTTTGTTTTTGACATGGAAt                                                                                                                                              >  1:836920/1‑210 (MQ=255)                                                                                                                                                   cGCACATTCGGCGCGGATGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCACCCAGCCCCATCAGGCAAAGTGCGGTTATAATTTTTTTTGTTTTTGACATGGAATCACTCCGATGTTTGAGCGTTTTAATAACTAAAAGGAATTTATAAc                                                                                                 <  1:383212/251‑1 (MQ=255)                                                                                                                                                             gcgcGGATGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCACCCAGCCCCATCAGGCAAAGTGCGGTTATAATTTTTTTTGTTTTTGACATGGAATCACTCCGATGTTTGAGCGTTTTAATAACTAAAAGGAATTTATAACTACAACGAg                                                                                        <  1:3278/250‑1 (MQ=255)                                                                                                                                                               gcgGATGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCACCCAGCCCCATCAGGCAAAGTGCGGTTATAATTTTTTTTGTTTTTGACATGGAATCACTCCGATGTTTGAGCGTTTTAATAACTAAAAGGAATTTATAACTAc                                                                                              >  1:273948/1‑242 (MQ=255)                                                                                                                                                                   aTGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCACCCAGCCCCATCAGGCAAAGTGCGGTTATAATTTTTTTTGTTTTTGACATGGAATCACTCCGATGTTTGAGCGTTTTAATAACTAAAAGGAATTTATAACTACAACGAGTTAgtgt                                                                                 <  1:71848/251‑1 (MQ=255)                                                                                                                                                                      ccTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCACCCAGCCCCATCAGGCAAAGTGCGGTTATAATTTTTTTTGTTTTTGACATGGAATCACTCCGATGTTTGAGCGTTTTAATAACTAAAAGGAATTTATAACTACAAc                                                                                           >  1:303615/1‑238 (MQ=255)                                                                                                                                                                        tttGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCACCCAGCCCCATCAGGCAAAGTGCGGTTATAATTTTTTTTGTTTTTGACATGGAATCACTCCGATGTTTGAGCGTTTTAATAACTAAAAGGAATTTATAACTACAACGAGTTAGTGTTGAtt                                                                            <  1:196131/251‑1 (MQ=255)                                                                                                                                                                          tGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCACCCAGCCCCATCAGGCAAAGTGCGGTTATAATTTTTTTTGTTTTTGACATGGAATCACTCCGATGTTTGAGCGTTTTAATAACTAAAAGGAATTTATAACTACAACGAGTTAGTGTTGATTCt                                                                          <  1:223381/251‑1 (MQ=255)                                                                                                                                                                             cGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCACCCAGCCCCATCAGGCAAAGTGCGGTTATAATTTTTTTTGTTTTTGACATGGAATCACTCCGATGTTTGAGCGTTTTAATAACTAAAAGGAATTTATAACTACAACGAGTTAGTGTTGATTCTAg                                                                        <  1:712698/250‑1 (MQ=255)                                                                                                                                                                             cGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCACCCAGCCCCATCAGGCAAAGTGCGGTTATAATTTTTTTTGTTTTTGACATGGAATCACTCCGATGTTTGAGCGTTTTAATAACTAAAAGGAATTTATAACTACAACGAGTTAGTGTTGATTCTAg                                                                        <  1:100433/250‑1 (MQ=255)                                                                                                                                                                               ttGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCACCCAGCCCCATCAGGCAAAGTGCGGTTATAATTTTTTTTGTTTTTGACATGGAATCACTCCGATGTTTGAGCGTTTTAATAACTAAAAGGAATTTATAACTACAACGAGTTAGTGTTGATTCTAgtg                                                                      <  1:365896/250‑1 (MQ=255)                                                                                                                                                                               ttGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCACCCAGCCCCATCAGGCAAAGTGCGGTTATAATTTTTTTTGTTTTTGACATGGAATCACTCCGATGTTTGAGCGTTTTAATAACTAAAAGGAATTTATAACTACAACGAGTTAGTGTTGATTCTAgtg                                                                      <  1:772802/250‑1 (MQ=255)                                                                                                                                                                                        tGGCTGGATCCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCACCCAGCCCCATCAGGCAAAGTGCGGTTATAATTTTTTTTGTTTTTGACATGGAATCACTCCGATGTTTGAGCGTTTTAATAACTAAAAGGAATTTATAACTACAACGAGTTAGTGTTGATTCTAGTGTATCtaaata                                                            <  1:333167/251‑1 (MQ=255)                                                                                                                                                                                         ggCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCACCCAGCCCCATCAGGCAAAGTGCGGTTATAATTTTTTTTGTTTTTGACATGGAATCACTCCGATGTTTGAGCGTTTTAATAACTAAAAGGAATTTATAACTACAAc                                                                                           <  1:96539/219‑1 (MQ=255)                                                                                                                                                                                               aaCCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCACCCAGCCCCATCAGGCAAAGTGCGGTTATAATTTTTTTTGTTTTTGACATGGAATCACTCCGATGTTTGAGCGTTTTAATAACTAAAAGGAATTTATAACTACAACGAGTTAGTGTTGATTCTAGTGTATCTAAATATAAATAc                                                     <  1:85989/251‑1 (MQ=255)                                                                                                                                                                                                           ccTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCACCCAGCCCCATCAGGCAAAGTGCGGTTATAATTTTTTTTGTTTTTGACATGGAATCACTCCGATGTTTGAGCGTTTTAATAACTAAAAGGAATTTATAACTACAACGAg                                                                                        >  1:101344/1‑204 (MQ=255)                                                                                                                                                                                                              gctgcGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCACCCAGCCCCATCAGGCAAAGTGCGGTTATAATTTTTTTTGTTTTTGACATGGAATCACTCCGATGTTTGAGCGTTTTAATAACTAAAAGGAATTTATAACTACAACGAGTTAGTGTTGATTCTAGTGTATCTAAATATAAATACTTTGAttt                                             >  1:286397/1‑244 (MQ=255)                                                                                                                                                                                                                          tCATGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCACCCAGCCCCATCAGGCAAAGTGCGGTTATAATTTTTTTTGTTTTTGACATGGAATCACTCCGATGTTTGAGCGTTTTAATAACTAAAAGGAATTTATAACTACAACGAGTTAGTGTTGATTCTAGTGTATCTAAATATAAATACTTTGATTTCAGTCAAGTAAAATAaatt                          <  1:804684/251‑1 (MQ=255)                                                                                                                                                                                                                             tGGAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCACCCAGCCCCATCAGGCAAAGTGCGGTTATAATTTTTTTTGTTTTTGACATGGAATCACTCCGATGTTTGAGCGTTTTAATAACTAAAAGGAATTTATAACTACAACGAGTTAGTGTTGATTCTAGTGTATCTAAATATAAATAc                                                     >  1:855617/1‑221 (MQ=255)                                                                                                                                                                                                                               gAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCACCCAGCCCCATCAGGCAAAGTGCGGTTATAATTTTTTTTGTTTTTGACATGGAATCACTCCGATGTTTGAGCGTTTTAATAACTAAAAGGAATTTATAACTACAACGAGTTAGTGTTGATTCTAgtg                                                                      >  1:625052/1‑202 (MQ=255)                                                                                                                                                                                                                               gAATGGCAGCTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCACCCAGCCCCATCAGGCAAAGTGCGGTTATAATTTTTTTTGTTTTTGACATGGAATCACTCCGATGTTTGAGCGTTTTAATAACTAAAAGGAATTTATAACTACAACGAGTTAGTGTTGATTCTAGTGTATCTAAATATAAATACTTTGATTTCAGTCAAGTaa                                  <  1:355909/238‑1 (MQ=255)                                                                                                                                                                                                                                        cTGACACAAAATTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCACCCAGCCCCATCAGGCAAAGTGCGGTTATAATTTTTTTTGTTTTTGACATGGAATCACTCCGATGTTTGAGCGTTTTAATAACTAAAAGGAATTTATAACTACAACGAGTTAGTGTTGATTCTAGTGTATCTAAATATAAATACTTTGATTTCAGTCAAGTAAAATAAATTAATTCCTTTATTTc            <  1:323332/251‑1 (MQ=255)                                                                                                                                                                                                                                                aaaTTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCACCCAGCCCCATCAGGCAAAGTGCGGTTATAATTTTTTTTGTTTTTGACATGGAATCACTCCGATGTTTGAGCGTTTTAATAACTAAAAGGAATTTATAACTACAACGAGTTAGTGTTGATTCTAGTGTAt                                                                   <  1:216961/188‑1 (MQ=255)                                                                                                                                                                                                                                                aaaTTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCACCCAGCCCCATCAGGCAAAGTGCGGTTATAATTTTTTTTGTTTTTGACATGGAATCACTCCGATGTTTGAGCGTTTTAATAACTAAAAGGAATTTATAACTACAACGAGTTAGTGTTGATTCTAGTGTATCTAAATATAAATACTTTGATTTCAGTCAAGTAAAATAAATTAATTCCTTTATTTCCTTATaaa    >  1:77792/1‑251 (MQ=255)                                                                                                                                                                                                                                                  aTTCCAGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCACCCAGCCCCATCAGGCAAAGTGCGGTTATAATTTTTTTTGTTTTTGACATGGAATCACTCCGATGTTTGAGCGTTTTAATAACTAAAAGGAATTTATAACTACAACGAGTTAGTGTTGATTCTAGTGTATCTAAATATAAATACTTTGATTTCAGTCAAGTAAAATAAATTAATTCCTTTATTTCCTTATAAATc  <  1:644397/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      TTCCACACGCGCTGCGCCATTTCCGCACGGTGTTCTTCGTATTTTTCTTTGCTGTCCAATTTGCCCTGCATTTCGTACCAAATATCTTTCAACGCAATGAATTTGGCTTTGACATAATGCCAGCCTTCTTGCGGTACGTGACAATCCGCACATTCGGCGCGGATGCCTTTGGCGTTGGCAAAATGGCTGGAACCTTCCCATTCCTGCTGCGGATGGCTCATGGAATGGCAGCTGACACAAAATTCCGGCGTGCCGGTTTCGTGCATGACAACCTGCGTTCCCCACAACGCACCGGCACCCAGCCCCATCAGGCAAAGTGCGGTTATAATTTTTTTTGTTTTTGACATGGAATCACTCCGATGTTTGAGCGTTTTAATAACTAAAAGGAATTTATAACTACAACGAGTTAGTGTTGATTCTAGTGTATCTAAATATAAATACTTTGATTTCAGTCAAGTAAAATAAATTAATTCCTTTATTTCCTTATAAATC  >  1/1175043‑1175534

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑