Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,168,301	G→A	intergenic (+8721/+252636)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,168,301	0	G	A	100.0%	137.5 / NA	48	intergenic (+8721/+252636)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (42/6);  total (42/6)

ATTAATGCACGACGCGGGACAGGCGCTGCTCGGCGAAAATGATTTCAGCTCCTTCCGCGCCGCTCAATGCCAATCCAACACCCCATGGCGCAACGTCCATCATTTAAACGTCACTCGTCAGGGGCAGTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAATATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTGAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGAAAATGCCAATGGGACCGTTGTTTCTGGCGGATTGATGATTGAATAGATTAAAGCCTGCTTTTGGCTTTGGTATAAACAAAATTTCGATCGCGTTTTCCTACGGCGATGATTTTGACCACAATGCGTT  >  1/1168075‑1168542                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                  aTTAATGCACGACGCGGGACAGGCGCTGCTCGGCGAAAATGATTTCAGCTCCTTCCGCGCCGCTCAATGCCAATCCAACACCCCATGGCGCAACGTCCATCATTTAAACGTCACTCGTCAGGGGCAGTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAg                                                                                                                                                                                                                            >  1:26395/1‑250 (MQ=255)  ttAATGCACGACGCGGGACAGGCGCTGCTCGGCGAAAATGATTTCAGCTCCTTCCGCGCCGCTCAATGCCAATCCAACACCCCATGGCGCAACGTCCATCATTTAAACGTCACTCGTCAGGGGCAGTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCa                                                                                                                                                                                                                          >  1:179502/1‑251 (MQ=255)            acgCGGGACAGGCGCTGCTCGGCGAAAATGATTTCAGCTCCTTCCGCGCCGCTCAATGCCAATCCAACACCCCATGGCGCAACGTCCATCATTTAAACGTCACTCGTCAGGGGCAGTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACcg                                                                                                                                                                                                                 >  1:642268/1‑250 (MQ=255)                      ggCGCTGCTCGGCGAAAATGATTTCAGCTCCTTCCGCGCCGCTCAATGCCAATCCAACACCCCATGGCGCAACGTCCATCATTTAAACGTCACTCGTCAGGGGCAGTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTcgccg                                                                                                                                                                                                      >  1:401969/1‑251 (MQ=255)                            gctCGGCGAAAATGATTTCAGCTCCTTCCGCGCCGCTCAATGCCAATCCAACACCCCATGGCGCAACGTCCATCATTTAAACGTCACTCGTCAGGGGCAGTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCa                                                                                                                                                                                                >  1:380349/1‑251 (MQ=255)                                         gATTTCAGCTCCTTCCGCGCCGCTCAATGCCAATCCAACACCCCATGGCGCAACGTCCATCATTTAAACGTCACTCGTCAGGGGCAGTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGa                                                                                                                                                                                   >  1:740363/1‑251 (MQ=255)                                                    cTTCCGCGCCGCTCAATGCCAATCCAACACCCCATGGCGCAACGTCCATCATTTAAACGTCACTCGTCAGGGGCAGTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTAt                                                                                                                                                                         >  1:38161/1‑250 (MQ=255)                                                              gcTCAATGCCAATCCAACACCCCATGGCGCAACGTCCATCATTTAAACGTCACTCGTCAGGGGCAGTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGt                                                                                                                                                              >  1:468437/1‑251 (MQ=255)                                                                   aTGCCAATCCAACACCCCATGGCGCAACGTCCATCATTTAAACGTCACTCGTCAGGGGCAGTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCAttt                                                                                                                                                         >  1:773272/1‑251 (MQ=255)                                                                         aTCCAACACCCCATGGCGCAACGTCCATCATTTAAACGTCACTCGTCAGGGGCAGTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCg                                                                                                                                                   >  1:85365/1‑251 (MQ=255)                                                                         aTCCAACACCCCATGGCGCAACGTCCATCATTTAAACGTCACTCGTCAGGGGCAGTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCg                                                                                                                                                   >  1:250161/1‑251 (MQ=255)                                                                                     aTGGCGCAACGTCCATCATTTAAACGTCACTCGTCAGGGGCAGTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGaa                                                                                                                                                                                                                                         >  1:292586/1‑153 (MQ=255)                                                                                              cGTCCATCATTTAAACGTCACTCGTCAGGGGCAGTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGaaaa                                                                                                                              >  1:883034/1‑251 (MQ=255)                                                                                                      atTTAAACGTCACTCGTCAGGGGCAGTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCt                                                                                                                                                                       >  1:295206/1‑202 (MQ=255)                                                                                                               tCACTCGTCAGGGGCAGTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGCGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCACTTACCCCGACAACTTTAATCTGCCGAAAATGCCAATGGGACCgttg                                                                                                             >  1:200696/1‑251 (MQ=255)                                                                                                                    cGTCAGGGGCAGTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGAAAATGCCAATGGGACCGTTGttt                                                                                                          >  1:754283/1‑249 (MQ=255)                                                                                                                    cGTCAGGGGCAGTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGAAAATGCCAATGGGACCGTTGTTTc                                                                                                         >  1:847183/1‑250 (MQ=255)                                                                                                                         ggggCAGTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGAAAATGCCAATGGGACCGTTGTTTCTGGCgg                                                                                                   >  1:556808/1‑251 (MQ=255)                                                                                                                               gTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTAccc                                                                                                                                                     >  1:264537/1‑195 (MQ=255)                                                                                                                               gTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGAAAATGCCAATGGGACCGTTGTTTCTGGCGGATtgat                                                                                             >  1:273603/1‑251 (MQ=255)                                                                                                                                 aCATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGAAAATGCCAATGGGACCGTTGTTTCTGGCGGATtgatga                                                                                           >  1:871869/1‑251 (MQ=255)                                                                                                                                 aCATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGAAAATGCCAATGGGACCGTTGTTTCTGGCGGATtgatga                                                                                           >  1:367735/1‑251 (MQ=255)                                                                                                                                 aCATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGAAAATGCCAATGGGACCGTTGTTTCTGGCGGATtgatga                                                                                           >  1:509207/1‑251 (MQ=255)                                                                                                                                 aCATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGAAAATGCCAATGGGACCGTTGTTTCTGGCGGATtgatga                                                                                           >  1:534665/1‑251 (MQ=255)                                                                                                                                               atTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGAAAATGCCAATGGGACCGTTGTTTCTGGCGGATTGATGATTGAATAGATTaaa                                                                             >  1:784667/1‑251 (MQ=255)                                                                                                                                                                   gCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATc                                                                                                                                                                        >  1:494527/1‑140 (MQ=255)                                                                                                                                                                   gCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGAAAATGCCAATGGGACCGTTGTTTCTGGCGGATTGATGATTGAAt                                                                                     <  1:70926/223‑1 (MQ=255)                                                                                                                                                                        acaTGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGAAAATGCCAATGGGACCGTTGTTTCTGGCGGATTGATGATTGAATAGATTAAAGCCTGCTTTTGGCTTTGGTATaaa                                                     >  1:811933/1‑250 (MQ=255)                                                                                                                                                                             gTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGAAAATGCCAATgg                                                                                                                     >  1:38724/1‑181 (MQ=255)                                                                                                                                                                             gTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGAAAATGCCAATGGGACCGTTGTTTCTGGCGGATTGATGATTGaa                                                                                      >  1:565112/1‑212 (MQ=255)                                                                                                                                                                             gTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGAAAATGCCAATGGGACCGTTGTTTCTGGCGGATTGATGATTGAATAGATTAAAGCCTGCTTTTGGCTTTGGTATAAACAAAAt                                               >  1:468872/1‑251 (MQ=255)                                                                                                                                                                             gTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGAAAATGCCAATGGGACCGTTGTTTCTGGCGGATTGATGATTGAATAGATTAAAGCCTGCTTTTGGCTTTGGTATAAACAAAAt                                               >  1:266253/1‑251 (MQ=255)                                                                                                                                                                             gTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGAAAATGCCAATGGGACCGTTGTTTCTGGCGGATTGATGATTGAATAGATTAAAGCCTGCTTTTGGCTTTGGTATAAACAAAAt                                               >  1:163733/1‑251 (MQ=255)                                                                                                                                                                               gCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGAAACTGCCAATGGGACCGTTGTTTCTGGCGGATTGATGATTGAATAGATTAAAGCCTGCTTTTGGCTTTGGTATAAACAAAAttt                                             >  1:428207/1‑251 (MQ=255)                                                                                                                                                                                 gTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGAAAATGCCAATGGGACCGTTGTTTCTGGCGGATTGATGATTGAATAGATTAAAGCCTGCTTTTGGCTTTGGTATAAAc                                                    >  1:233214/1‑242 (MQ=255)                                                                                                                                                                                         gtcgGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGAAAATGCCAATGGGACCGTTGTTTCTGGCGGATTGATGATTGAATAGATTAAAGCCTGCTTTTGGCTTTGGTATAAACAAAATTTCGATCGCGtt                                   >  1:170882/1‑251 (MQ=255)                                                                                                                                                                                           cgGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATc                                                                                                                                                                        <  1:757697/116‑1 (MQ=255)                                                                                                                                                                                                   ttATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTAccc                                                                                                                                                     >  1:625205/1‑127 (MQ=255)                                                                                                                                                                                                   ttATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGAAAATGcc                                                                                                                          <  1:198788/154‑1 (MQ=255)                                                                                                                                                                                                   ttATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGAAAATGCCAATGGGACCGTTGTTTCTGGCGGATTGATGATTGAATAGATTAAAGCCTGCTTTTGGCTTTGGTATAAACAAAATTTCGATCGCGTTTTCCTACgg                          >  1:548472/1‑250 (MQ=255)                                                                                                                                                                                                   ttATTGAAGTGGGCTGTTACAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATc                                                                                                                                       <  1:737878/141‑1 (MQ=255)                                                                                                                                                                                                        gAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGAAAATGCCAATGGGACCGTTGTTTCTGGCGGATTGATGATTGAATAGATTAAAGCCTGCTTTTGGCTTTGGTATAAACAAAATTTCGATCGCGTTTTCCTACGGCgatga                    >  1:326184/1‑251 (MQ=255)                                                                                                                                                                                                              ggCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGAAAATGCCAATGGGACCGTTGTTTCTGGCGGATTGATGATTGAATAGATTAAAGCCTGCTTTTGGCTTTGGTATAAACAAAATTTCGATCGCGTTTTCCTACGGCGATGATTTTGa              >  1:191990/1‑251 (MQ=255)                                                                                                                                                                                                                  gTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGAAAATGCCAATGGGACCGTTGTTTCTGGCGGATTGATGGTTGAATAGATTAAAGCCTGCTTTTGGCTTTGGTATAAACAAAATTTCGATCGCGTTTTCCTACGGCGATGATTTTGACca           >  1:446858/1‑250 (MQ=255)                                                                                                                                                                                                                  gTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGAAAATGCCAATGGGACCGTTGTTTCTGGCGGATTGATGATTGAATAGATTAAAGCCTGCTTTTGGCTTTGGTATAAACAAAATTTCGATCGCGTTTTCCTACGGCGATGATTTTGACca           >  1:580879/1‑250 (MQ=255)                                                                                                                                                                                                                     gCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGGCCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGAAAATGCCAATGGGACCGTTGTTTCTGGCGGATTGATGATTGAATAGATTAAAGCCTGCTTTTGGCTTTGGTATAAACAAAATTTCGATCGCGTTTTCCTACgg                          <  1:93485/232‑1 (MQ=255)                                                                                                                                                                                                                     gCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGAAAATGCCAATGGGACCGTTGTTTCTGGCGGATTGATGATTGAATAGATTAAAGCCTGCTTTTGGCTTTGGTATAAACAAAATTTCGATCGCGTTTTCCTACGGCGATGATTTTGACCACAAt       >  1:852724/1‑251 (MQ=255)                                                                                                                                                                                                                          cAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGAAAATGCCAATGGGACCGTTGTTTCTGGCGGATTGATGATTGAATAGATTAAAGCCTGCTTTTGGCTTTGGTATAAACAAAATTTCGATCGCGTTTTCCTACGGCGATGATTTTGACCACAATGCGtt  <  1:521865/251‑1 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                  ATTAATGCACGACGCGGGACAGGCGCTGCTCGGCGAAAATGATTTCAGCTCCTTCCGCGCCGCTCAATGCCAATCCAACACCCCATGGCGCAACGTCCATCATTTAAACGTCACTCGTCAGGGGCAGTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAATATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTGAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGAAAATGCCAATGGGACCGTTGTTTCTGGCGGATTGATGATTGAATAGATTAAAGCCTGCTTTTGGCTTTGGTATAAACAAAATTTCGATCGCGTTTTCCTACGGCGATGATTTTGACCACAATGCGTT  >  1/1168075‑1168542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑