Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,168,255	T→C	intergenic (+8675/+252682)	unknown → / ← unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,168,255	0	T	C	100.0%	119.0 / NA	43	intergenic (+8675/+252682)	unknown/unknown	tRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (42/1);  total (42/1)

CGGCGATTTTGCCGCAAGGCGTAACCCATTGCCATTTAGAACTCGATCATCAATTAATGCACGACGCGGGACAGGCGCTGCTCGGCGAAAATGATTTCAGCTCCTTCCGCGCCGCTCAATGCCAATCCAACACCCCATGGCGCAACGTCCATCATTTAAACGTCACTCGTCAGGGGCAGTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAATATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTGAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGAAAATGCCAATGGGACCGTTGTTTCTGGCGGATTGATGATTGAATAGATTAAAGCCTGCTTTTGGCTTTGGTATAAACAAAATTT  >  1/1168023‑1168499                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                     cGGCGATTTTGCCGCAAGGCGTAACCCATTGCCATTTAGAACTCGATCATCAATTAATGCACGACGCGGGACAGGCGCTGCTCGGCGAAAATGATTTCAGCTCCTTCCGCGCCGCTCAATGCCAATCCAACACCCCATGGCGCAACGTCCATCATTTAAACGTCACTCGTCAGGGGCAGTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTAtt                                                                                                                                                                                                                                    >  1:293021/1‑251 (MQ=255)                 aGGCGTAACCCATTGCCATTTAGAACTCGATCATCAATTAATGCACGACGCGGGACAGGCGCTGCTCGGCGAAAATGATCTCAGCCCCTTCCGCGCCGCTCAATGCCAATCCAACACCCCATGGCGCAACGTCCATCATTTAAACGTCACTCGTCAGGGGCAGCACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCa                                                                                                                                                                                                                    >  1:391475/1‑251 (MQ=255)                   gCGTAACCCATTGCCATTTAGAACTCGATCATCAATTAATGCACGACGCGGGACAGGCGCTGCTCGGCGAAAATGATTTCAGCTCCTTCCGCGCCGCTCAATGCCAATCCAACACCCCATGGCGCAACGTCCATCATTTAAACGTCACTCGTCAGGGGCAGTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAAt                                                                                                                                                                                                                  >  1:459042/1‑251 (MQ=255)                   gCGTAACCCATTGCCATTTAGAACTCGATCATCAATTAATGCACGACGCGGGACAGGCGCTGCTCGGCGAAAATGATTTCAGCTCCTTCCGCGCCGCTCAATGCCAATCCAACACCCCATGGCGCAACGTCCATCATTTAAACGTCACTCGTCAGGGGCAGTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAAt                                                                                                                                                                                                                  >  1:267514/1‑251 (MQ=255)                        aCCCATTGCCATTTAGAACTCGATCATCAATTAATGCACGACGCGGGACAGGCGCTGCTCGGCGAAAATGATTTCAGCTCCTTCCGCGCCGCTCAATGCCAATCCAACACCCCATGGCGCAACGTCCATCATTTAAACGTCACTCGTCAGGGGCAGTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAAc                                                                                                                                                                                                              >  1:784767/1‑250 (MQ=255)                                                  tcaATTAATGCACGACGCGGGACAGGCGCTGCTCGGCGAAAATGATTTCAGCTCCTTCCGCGCCGCTCAATGCCAATCCAACACCCCATGGCGCAACGTCCATCATTTAAACGTCACTCGTCAGGGGCAGTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTg                                                                                                                                                                                                                                   >  1:585538/1‑203 (MQ=255)                                                     aTTAATGCACGACGCGGGACAGGCGCTGCTCGGCGAAAATGATTTCAGCTCCTTCCGCGCCGCTCAATGCCAATCCAACACCCCATGGCGCAACGTCCATCATTTAAACGTCACTCGTCAGGGGCAGTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAg                                                                                                                                                                                 >  1:26395/1‑250 (MQ=255)                                                      ttAATGCACGACGCGGGACAGGCGCTGCTCGGCGAAAATGATTTCAGCTCCTTCCGCGCCGCTCAATGCCAATCCAACACCCCATGGCGCAACGTCCATCATTTAAACGTCACTCGTCAGGGGCAGTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCa                                                                                                                                                                               >  1:179502/1‑251 (MQ=255)                                                                acgCGGGACAGGCGCTGCTCGGCGAAAATGATTTCAGCTCCTTCCGCGCCGCTCAATGCCAATCCAACACCCCATGGCGCAACGTCCATCATTTAAACGTCACTCGTCAGGGGCAGTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACcg                                                                                                                                                                      >  1:642268/1‑250 (MQ=255)                                                                          ggCGCTGCTCGGCGAAAATGATTTCAGCTCCTTCCGCGCCGCTCAATGCCAATCCAACACCCCATGGCGCAACGTCCATCATTTAAACGTCACTCGTCAGGGGCAGTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTcgccg                                                                                                                                                           >  1:401969/1‑251 (MQ=255)                                                                                gctCGGCGAAAATGATTTCAGCTCCTTCCGCGCCGCTCAATGCCAATCCAACACCCCATGGCGCAACGTCCATCATTTAAACGTCACTCGTCAGGGGCAGTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCa                                                                                                                                                     >  1:380349/1‑251 (MQ=255)                                                                                             gATTTCAGCTCCTTCCGCGCCGCTCAATGCCAATCCAACACCCCATGGCGCAACGTCCATCATTTAAACGTCACTCGTCAGGGGCAGTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTg                                                                                                                                                                                                                                   >  1:136127/1‑160 (MQ=255)                                                                                             gATTTCAGCTCCTTCCGCGCCGCTCAATGCCAATCCAACACCCCATGGCGCAACGTCCATCATTTAAACGTCACTCGTCAGGGGCAGTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGa                                                                                                                                        >  1:740363/1‑251 (MQ=255)                                                                                                        cTTCCGCGCCGCTCAATGCCAATCCAACACCCCATGGCGCAACGTCCATCATTTAAACGTCACTCGTCAGGGGCAGTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTAt                                                                                                                              >  1:38161/1‑250 (MQ=255)                                                                                                                  gcTCAATGCCAATCCAACACCCCATGGCGCAACGTCCATCATTTAAACGTCACTCGTCAGGGGCAGTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGt                                                                                                                   >  1:468437/1‑251 (MQ=255)                                                                                                                       aTGCCAATCCAACACCCCATGGCGCAACGTCCATCATTTAAACGTCACTCGTCAGGGGCAGTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCAttt                                                                                                              >  1:773272/1‑251 (MQ=255)                                                                                                                             aTCCAACACCCCATGGCGCAACGTCCATCATTTAAACGTCACTCGTCAGGGGCAGTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCg                                                                                                        >  1:250161/1‑251 (MQ=255)                                                                                                                             aTCCAACACCCCATGGCGCAACGTCCATCATTTAAACGTCACTCGTCAGGGGCAGTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCg                                                                                                        >  1:85365/1‑251 (MQ=255)                                                                                                                                         aTGGCGCAACGTCCATCATTTAAACGTCACTCGTCAGGGGCAGTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGaa                                                                                                                                                                                              >  1:292586/1‑153 (MQ=255)                                                                                                                                                  cGTCCATCATTTAAACGTCACTCGTCAGGGGCAGTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGaaaa                                                                                   >  1:883034/1‑251 (MQ=255)                                                                                                                                                          atTTAAACGTCACTCGTCAGGGGCAGTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGa                                                                                                                                                                                                                                  >  1:634661/1‑100 (MQ=255)                                                                                                                                                          atTTAAACGTCACTCGTCAGGGGCAGTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCt                                                                                                                            >  1:295206/1‑202 (MQ=255)                                                                                                                                                                   tCACTCGTCAGGGGCAGTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGCGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCACTTACCCCGACAACTTTAATCTGCCGAAAATGCCAATGGGACCgttg                                                                  >  1:200696/1‑251 (MQ=255)                                                                                                                                                                        cGTCAGGGGCAGTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGAAAATGCCAATGGGACCGTTGttt                                                               >  1:754283/1‑249 (MQ=255)                                                                                                                                                                        cGTCAGGGGCAGTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGAAAATGCCAATGGGACCGTTGTTTc                                                              >  1:847183/1‑250 (MQ=255)                                                                                                                                                                             ggggCAGTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGAAAATGCCAATGGGACCGTTGTTTCTGGCgg                                                        >  1:556808/1‑251 (MQ=255)                                                                                                                                                                                   gTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTAccc                                                                                                          >  1:264537/1‑195 (MQ=255)                                                                                                                                                                                   gTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGAAAATGCCAATGGGACCGTTGTTTCTGGCGGATtgat                                                  >  1:273603/1‑251 (MQ=255)                                                                                                                                                                                     aCATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGAAAATGCCAATGGGACCGTTGTTTCTGGCGGATtgatga                                                >  1:871869/1‑251 (MQ=255)                                                                                                                                                                                     aCATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGAAAATGCCAATGGGACCGTTGTTTCTGGCGGATtgatga                                                >  1:534665/1‑251 (MQ=255)                                                                                                                                                                                     aCATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGAAAATGCCAATGGGACCGTTGTTTCTGGCGGATtgatga                                                >  1:509207/1‑251 (MQ=255)                                                                                                                                                                                     aCATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGAAAATGCCAATGGGACCGTTGTTTCTGGCGGATtgatga                                                >  1:367735/1‑251 (MQ=255)                                                                                                                                                                                                   atTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGAAAATGCCAATGGGACCGTTGTTTCTGGCGGATTGATGATTGAATAGATTaaa                                  >  1:784667/1‑251 (MQ=255)                                                                                                                                                                                                                       gCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATc                                                                                                                             >  1:494527/1‑140 (MQ=255)                                                                                                                                                                                                                       gCATCACATGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGAAAATGCCAATGGGACCGTTGTTTCTGGCGGATTGATGATTGAAt                                          <  1:70926/223‑1 (MQ=255)                                                                                                                                                                                                                            acaTGGTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGAAAATGCCAATGGGACCGTTGTTTCTGGCGGATTGATGATTGAATAGATTAAAGCCTGCTTTTGGCTTTGGTATaaa          >  1:811933/1‑250 (MQ=255)                                                                                                                                                                                                                                 gTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGAAAATGCCAATgg                                                                          >  1:38724/1‑181 (MQ=255)                                                                                                                                                                                                                                 gTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGAAAATGCCAATGGGACCGTTGTTTCTGGCGGATTGATGATTGaa                                           >  1:565112/1‑212 (MQ=255)                                                                                                                                                                                                                                 gTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGAAAATGCCAATGGGACCGTTGTTTCTGGCGGATTGATGATTGAATAGATTAAAGCCTGCTTTTGGCTTTGGTATAAACAAAAt    >  1:266253/1‑251 (MQ=255)                                                                                                                                                                                                                                 gTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGAAAATGCCAATGGGACCGTTGTTTCTGGCGGATTGATGATTGAATAGATTAAAGCCTGCTTTTGGCTTTGGTATAAACAAAAt    >  1:468872/1‑251 (MQ=255)                                                                                                                                                                                                                                 gTGCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGAAAATGCCAATGGGACCGTTGTTTCTGGCGGATTGATGATTGAATAGATTAAAGCCTGCTTTTGGCTTTGGTATAAACAAAAt    >  1:163733/1‑251 (MQ=255)                                                                                                                                                                                                                                   gCGTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGAAACTGCCAATGGGACCGTTGTTTCTGGCGGATTGATGATTGAATAGATTAAAGCCTGCTTTTGGCTTTGGTATAAACAAAAttt  >  1:428207/1‑251 (MQ=255)                                                                                                                                                                                                                                     gTAACATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTAAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGAAAATGCCAATGGGACCGTTGTTTCTGGCGGATTGATGATTGAATAGATTAAAGCCTGCTTTTGGCTTTGGTATAAAc         >  1:233214/1‑242 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                     CGGCGATTTTGCCGCAAGGCGTAACCCATTGCCATTTAGAACTCGATCATCAATTAATGCACGACGCGGGACAGGCGCTGCTCGGCGAAAATGATTTCAGCTCCTTCCGCGCCGCTCAATGCCAATCCAACACCCCATGGCGCAACGTCCATCATTTAAACGTCACTCGTCAGGGGCAGTACATTATCGTGGATATTCAAGCTAACGCTTTTGTGCATCACATGGTGCGTAATATCGTCGGCAGCCTTATTGAAGTGGGCTGTTGCAATCAACCCGTTGAGTGGATGAAATGGTTGTTGGAGCAAAAAGACCGCGCCCTCGCCGCGCCCACCGCGAAACCGGAAGGGTTGTATCTGGTGAATGTCATTTACCCCGACAACTTTAATCTGCCGAAAATGCCAATGGGACCGTTGTTTCTGGCGGATTGATGATTGAATAGATTAAAGCCTGCTTTTGGCTTTGGTATAAACAAAATTT  >  1/1168023‑1168499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑