Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 1,160,338 C→T intergenic (+758/+260599) unknown → / ← unknown tRNA‑Val‑GAC/tRNA‑Met‑CAT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,160,3380CT98.7% 187.9 / NA 79intergenic (+758/+260599)unknown/unknowntRNA‑Val‑GAC/tRNA‑Met‑CAT
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (47/30);  total (49/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01

AATGAGGATATTGGGCGGAGCGGGTTTCCGCCCTTGTTTTATTCAATATTTTATTTTTATTCACCGGTAACCATGCAAGCACTCAATCCGATTTCCATTCCGTTAAACGCCATCAGTCTTATTGAAGCCTCTGCAGGGACGGGCAAAACCTACACCATGGGTTCTCTTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTAGCGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAACGCAGGATCCGACGGTGTTTGAACAAGATGACTTTTTACGCCAATTAGCCGATACCATCACGGATTTCCCTCTTGCCATTCAGCGCCTGACTTTGGCGGAACAAAACATGGATTTGGCGGCGATTTATACCATTCATGGATTTTGCC  >  1/1160100‑1160580
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                  
aaTGAGGATATTGGGCGGAGCGGGTTTCCGCCCTTTTTTTATTCAATATTTTATTTTTATTCACCGGTAACCATGCAAGCACTCAATCCGATTTCCATTCCGTTAAACGCCATCAGTCTTATTGAAGCCTCTGCAGGGACGGGCAAAACCTACACCATGGGTTCTCTTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTa                                                                                                                                                                                                                                         >  1:504394/1‑250 (MQ=255)
 aTGAGGATATTGGGCGGAGCGGGTTTCCGCCCTTTTTTTATTCAATATTTTATTTTTATTCACCGGTAACCATGCAAGCACTCAATCCGATTTCCATTCCGTTAAACGCCATCAGTCTTATTGAAGCCTCTGCAGGGACGGGCAAAACCTACACCATGGGTTCTCTTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTAcc                                                                                                                                                                                                                                       <  1:154587/251‑1 (MQ=255)
       atatTGGGCGGAGCGGGTTTCCGCCCTTTTTTTATTCAATATTTTATTTTTATTCACCGGTAACCATGCAAGCACTCAATCCGATTTCCATTCCGTTAAACGCCATCAGTCTTATTGAAGCCTCTGCAGGGACGGGCAAAACCTACACCATGGGTTCTCTTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATg                                                                                                                                                                                                                                 >  1:740829/1‑251 (MQ=255)
       atatTGGGCGGAGCGGGTTTCCGCCCTTTTTTTATTCAATATTTTATTTTTATTCACCGGTAACCATGCAAGCACTCAATCCGATTTCCATTCCGTTAAACGCCATCAGTCTTATTGAAGCCTCTGCAGGGACGGGCAAAACCTACACCATGGGTTCTCTTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATg                                                                                                                                                                                                                                 >  1:502381/1‑251 (MQ=255)
        tatTGGGCGGAGCGGGTTTCCGCCCTTTTTTTATTCAATATTTTATTTTTATTCACCGGTAACCATGCAAGCACTCAATCCGATTTCCATTCCGTTAAACGCCATCAGTCTTATTGAAGCCTCTGCAGGGACGGGCAAAACCTACACCATGGGTTCTCTTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATgg                                                                                                                                                                                                                                >  1:172490/1‑251 (MQ=255)
                        tttCCGCCCTTTTTTTATTCAATATTTTATTTTTATTCACCGGTAACCATGCAAGCACTCAATCCGATTTCCATTCCGTTAAACGCCATCAGTCTTATTGAAGCCTCTGCAGGGACGGGCAAAACCTACACCATGGGTTCTCTTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGaa                                                                                                                                                                                                                <  1:138017/251‑1 (MQ=255)
                         ttCCGCCCTTTTTTTATTCAATATTTTATTTTTATTCACCGGTAACCATGCAAGCACTCAATCCGATTTCCATTCCGTTAAACGCCATCAGTCTTATTGAAGCCTCTGCAGGGACGGGCAAAACCTACACCATGGGTTCTCTTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGaaa                                                                                                                                                                                                               <  1:336446/251‑1 (MQ=255)
                              cccTTTTTTTATTCAATATTTTATTTTTATTCACCGGTAACCATGCAAGCACTCAATCCGATTTCCATTCCGTTAAACGCCATCAGTCTTATTGAAGCCTCTGCAGGGACGGGCAAAACCTACACCATGGGTTCTCTTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTTAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTaa                                                                                                                                                                                                          >  1:855592/1‑251 (MQ=255)
                              cccTTTTTTTATTCAATATTTTATTTTTATTCACCGGTAACCATGCAAGCACTCAATCCGATTTCCATTCCGTTAAACGCCATCAGTCTTATTGAAGCCTCTGCAGGGACGGGCAAAACCTACACCATGGGTTCTCTTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTaa                                                                                                                                                                                                          <  1:16782/251‑1 (MQ=255)
                                               attttattttTATTCACCGGTAACCATGCAAGCACTCAATCCGATTTCCATTCCGTTAAACGCCATCAGTCTTATTGAAGCCTCTGCAGGGACGGGCAAAACCTACACCATGGGTTCTCTTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGAttt                                                                                                                                                                                         >  1:700728/1‑251 (MQ=255)
                                                    atttttattCACCGGTAACCATGCAAGCACTCAATCCGATTTCCATTCCGTTAAACGCCATCAGTCTTATTGAAGCCTCTGCAGGGACGGGCAAAACCTACACCATGGGTTCTCTTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGAc                                                                                                                                                                                    <  1:221365/251‑1 (MQ=255)
                                                               ccGGTAACCATGCAAGCACTCAATCCGATTTCCATTCCGTTAAACGCCATCAGTCTTATTGAAGCCTCTGCAGGGACGGGCAAAACCTACACCATGGGTTCTCTTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACaaaa                                                                                                                                                                         >  1:45288/1‑251 (MQ=255)
                                                               ccGGTAACCATGCAAGCACTCAATCCGATTTCCATTCCGTTAAACGCCATCAGTCTTATTGAAGCCTCTGCAGGGACGGGCAAAACCTACACCATGGGTTCTCTTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAAAGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACaaaa                                                                                                                                                                         >  1:901566/1‑251 (MQ=255)
                                                               ccGGTAACCATGCAAGCACTCAATCCGATTTCCATTCCGTTAAACGCCATCAGTCTTATTGAAGCCTCTGCAGGGACGGGCAAAACCTACACCATGGGTTCTCTTTATTTGCGCCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAAc                                                                                                                                                                                                                    <  1:331645/208‑1 (MQ=255)
                                                                    aaCCATGCAAGCACTCAATCCGATTTCCATTCCGTTAAACGCCATCAGTCTTATTGAAGCCTCTGCAGGGACGGGCAAAACCTACACCATGGGTTCTCTTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGa                                                                                                                                                                    >  1:265797/1‑251 (MQ=255)
                                                                      ccATGCAAGCACTCAATCCGATTTCCATTCCGTTAAACGCCATCAGTCTTATTGAAGCCTCTGCAGGGACGGGCAAAACCTACACCATGGGTTCTCTTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATCCGTTTAATCTGGAGCAAATTTTGGTGGCGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAAATGACg                                                                                                                                                                  >  1:728560/1‑251 (MQ=255)
                                                                           cAAGCACTCAATCCGATTTCCATTCCGTTAAACGCCATCAGTCTTATTGAAGCCTCTGCAGGGACGGGCAAAACCTACACCATGGGTTCTCTTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTTGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTa                                                                                                                                                             >  1:28508/1‑251 (MQ=255)
                                                                               cACTCAATCCGATTTCCATTCCGTTAAACGCCATCAGTCTTATTGAAGCCTCTGCAGGGACGGGCAAAACCTACACCATGGGTTCTCTTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATcaa                                                                                                                                                         >  1:5046/1‑251 (MQ=255)
                                                                                   cAATCCGATTTCCATTCCGTTAAACGCCATCAGTCTTATTGAAGCCTCTGCAGGGACGGGCAAAACCTACACCATGGGTTCTCTTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACaaa                                                                                                                                                     >  1:724540/1‑251 (MQ=255)
                                                                                       ccGATTTCCATTCCGTTAAACGCCATCAGTCTTATTGAAGCCTCTGCAGGGACGGGCAAAACCTACACCATGGGTTCTCTTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGa                                                                                                                                                                                     >  1:804431/1‑215 (MQ=255)
                                                                                           tttCCATTCCGTTAAACGCCATCAGTCTTATTGAAGCCTCTGCAGGGACGGGCAAAACCTACACCATGGGTTCTCTTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAACGCAGGAt                                                                                                                                             <  1:342587/251‑1 (MQ=255)
                                                                                                aTTCCGTTAAACGCCATCAGTCTTATTGAAGCCTCTGCAGGGACGGGCAAAACCTACACCATGGGTTCTCTTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAACGCAGGATCCGAc                                                                                                                                        <  1:531739/251‑1 (MQ=255)
                                                                                                         aaCGCCATCAGTCTTATTGAAGCCTCTGCAGGGACGGGCAAAACCTACACCATGGGTTCTCTTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCCAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAACGCAGGATCCGACGGTGTTTGa                                                                                                                               >  1:3724/1‑251 (MQ=255)
                                                                                                          aCGCCATCAGTCTTATTGAAGCCTCTGCAGGGACGGGCAAAACCTACACCATGGGTTCTCTTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAACGCAGGATCCGACGGTGTTTGaa                                                                                                                              <  1:320546/251‑1 (MQ=255)
                                                                                                             ccATCAGTCTTATTGAAGCCTCTGCAGGGACGGGCAAAACCTACACCATGGGTTCTCTTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAACGCAGGATCCGACGGTGTTTGAACa                                                                                                                            >  1:540925/1‑250 (MQ=255)
                                                                                                                   gTCTTATTGAAGCCTCTGCAGGGACGGGCAAAACCTACACCATGGGTTCTCTTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCg                                                                                                                                                                                                                                      >  1:652032/1‑138 (MQ=255)
                                                                                                                   gTCTTATTGAAGCCTCTGCAGGGACGGGCAAAACCTACACCATGGGTTCTCTTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTg                                                                                                                                                                     >  1:241669/1‑203 (MQ=255)
                                                                                                                   gTCTTATTGAAGCCTCTGCAGGGACGGGCAAAACCTACACCATGGGTTCTCTTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGGGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAACGCAGGACCCGACGGTGTTTGAAAACGATGa                                                                                                                      >  1:633862/1‑250 (MQ=255)
                                                                                                                   gTCTTATTGAAGCCTCTGCAGGGACGGGCAAAACCTACACCATGGGTTCTCTTTATTTGCGACTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGa                                                                                                                                                                                     >  1:874102/1‑187 (MQ=255)
                                                                                                                      ttATTGAAGCCTCTGCAGGGACGGGCAAAACCTACACCATGGGTTCTCTTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTaa                                                                                                                                                                                                          >  1:153871/1‑163 (MQ=255)
                                                                                                                      ttATTGAAGCCTCTGCAGGGACGGGCAAAACCTACACCATGGGTTCTCTTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAACGCAGGATCCGACGGTGTTTGAACACGATGACttt                                                                                                                  >  1:477017/1‑251 (MQ=255)
                                                                                                                        aTTGAAGCCTCTGCAGGGACGGGCAAAACCTACACCATGGGTTCTCTTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAACGCAGGATCCGACGGTGTTTGAACACGATGACttttt                                                                                                                >  1:11206/1‑251 (MQ=255)
                                                                                                                            aaGCCTCTGCAGGGACGGGCAAAACCTACACCATGGGTTCTCTTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAACGCAGGATCCGACGGTGTTTGAACACGATGACTTTTTACGc                                                                                                            <  1:147579/251‑1 (MQ=255)
                                                                                                                               ccTCTGCAGGGACGGGCAAAACCTACACCATGGGTTCTCTTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAACGCAGGATCCGACGGTGTTTGAACACGATGACTTTTTACGcc                                                                                                           <  1:511767/249‑1 (MQ=255)
                                                                                                                               ccTCTGCAGGGACGGGCAAAACCTACACCATGGGTTCTCTTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAACGCAGGATCCGACGGTGTTTGAACACGATGACTTTTTACGCCaa                                                                                                         >  1:729034/1‑251 (MQ=255)
                                                                                                                               ccTCTGCAGGGACGGGCAAAACCTACACCATGGGTTCTCTTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAACGCAGGATCCGACGGGGTTTGAACACGATGACTTTTTACGCCaa                                                                                                         >  1:873078/1‑251 (MQ=255)
                                                                                                                                  ctGCAGGGACGGGCAAAACCTACACCATGGGTTCTCTTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAACGCAGGATCCGACGGTGTTTGAACACGATGACTTTTTACGCCAATTa                                                                                                      >  1:69515/1‑251 (MQ=255)
                                                                                                                                        ggACGGGCAAAACCTACACCATGGGTTCTCTTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAACGCAGGATCCGACGGTGTTTGAACACGATGACTTTTTACGCCAATTAGCCg                                                                                                  <  1:865298/249‑1 (MQ=255)
                                                                                                                                            gggCAAAACCTACACCATGGGTTCTCTTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCCTTTCTCATGTGTTTAATGTGGGGGAAATTTTGGCGGGGACCTTTACCGAAAATGCAACGGTGGAACTGAAACGTAAAATTTGCGAGCCGATTTATGACGACATACAAAAATTTACGGCGCAACCATAAAAGCAGGATCCGTCGGTGTTTGTACACGATCACTTTTTACGCCAAttttccattact                                                                                             >  1:235714/1‑240 (MQ=255)
                                                                                                                                             ggCAAAACCTACACCATGGGTTCTCTTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAACGCAGGATCCGACGGTGTTTGAACACGATGACTTTTTACGCCAATTAGCCGATACCAt                                                                                           >  1:75908/1‑251 (MQ=255)
                                                                                                                                                  aaCCTACACCATGGGTTCTCTTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCCAACAAAAATTGACGGCGTATCAACAAACGCAGGATc                                                                                                                                            >  1:17044/1‑197 (MQ=255)
                                                                                                                                                  aaCCTACACCATGGGTTCTCTTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAACGCAGGATCCGACgg                                                                                                                                      >  1:893480/1‑203 (MQ=255)
                                                                                                                                                  aaCCTACACCATGGGTTCTCTTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAACGCAGGATCCGACGGTGTTTGAAc                                                                                                                             >  1:502506/1‑212 (MQ=255)
                                                                                                                                                  aaCCTACACCATGGGTTCTCTTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAACGCAGGATCCGACGGTGTTTGAACACGATGACTTTTTACGCCAATTAGCCGATACCATCACgg                                                                                      >  1:377487/1‑251 (MQ=255)
                                                                                                                                                   aCCTACACCATGGGTTCTCTTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAACGCAGGATCCGACGGTGTTTGAACACGATGACTTTTTACGCCAATTAGCCGATACCATCACGGa                                                                                     <  1:839151/251‑1 (MQ=255)
                                                                                                                                                   aCCTACACCATGGGTTCTCTTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAACGCAGGATCCGACGGTGTTTGAACACGATGACTTTTTACGCCAATTAGCCGATACCATCACGGa                                                                                     >  1:321327/1‑251 (MQ=255)
                                                                                                                                                    ccTACACCATGGGTTCTCTTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTAACGGCGTATCAACAAACGCAGGATCCGACGGTGTTTGAACACGATGACTTTTTACGCCAATTAGCCGATACCATCACGGAt                                                                                    >  1:449202/1‑251 (MQ=255)
                                                                                                                                                       acacCATGGGTTCTCTTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAACGCAGGATCCGACGGTGTTTGAACACGATGACTTTTTACGCCAATTAGCCGATACCATCACGGAt                                                                                    <  1:588719/248‑1 (MQ=255)
                                                                                                                                                               ggTTCTCTTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAACGCAGGATCCGACGGTGTTTGAACACGATGACTTTTTACGCCAATTAGCCGATACCATCACGGATTTccc                                                                               <  1:24708/245‑1 (MQ=255)
                                                                                                                                                                gTTCTCTTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAAc                                                                                                                                                    <  1:281791/175‑1 (MQ=255)
                                                                                                                                                                    tctTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAACGCAGGATCCGACGGTGTTTGAACACGATGACTTTTTACGCCAATTAGCCGATACCATCACGGATTTCCCTCTTGCCATTc                                                                    <  1:263757/251‑1 (MQ=255)
                                                                                                                                                                     ctTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCCTGTGTTAAATGTGGAGCAAATTTTGGGGGTGACCTTTACCGAAATGGCAACGGAGGGACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAACGCAGGATCCGACGGGGTTTGAAc                                                                                                                             >  1:481198/1‑193 (MQ=255)
                                                                                                                                                                     ctTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAAc                                                                                                                                                                                                                    >  1:768055/1‑106 (MQ=255)
                                                                                                                                                                     ctTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAACGCAGGATCCGACGGTGTTTGAACACGATGACTTTTTACGCCAATTAGc                                                                                                    <  1:402882/218‑1 (MQ=255)
                                                                                                                                                                     ctTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAACGCAGGATCCGACGGTGTTTGAACACGATGACTTTTTACGCCAATTAGCCGATACCATCACGGATTTCCctc                                                                             <  1:69514/241‑1 (MQ=255)
                                                                                                                                                                       ttATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAACGCAGGATCCGACGGTGTTTGAACACGATGACTTTTTACGCCAATTAGCCGATACCATCa                                                                                         >  1:193366/1‑227 (MQ=255)
                                                                                                                                                                                tCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAACGCAGGATCCGACGGTGTTTGAACACGATGACTTTTTACGCCAATTAGCCGATACCATCACGGATTTCCCTCTTGCCATTCAGCGCCTGACt                                                         <  1:13937/250‑1 (MQ=255)
                                                                                                                                                                                    ttgttgCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAACGCAGGATCCGACGGTGTTTGAACACGATGACTTTTTACGCCAATTAGCCGATACCATCACGGATTTCCCTCTTGCCATTCAGCGCCTGACTTTGGc                                                    <  1:751172/251‑1 (MQ=255)
                                                                                                                                                                                       ttgCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAACGCAGGATCCGACGGTGTTTGAACACGATGACTTTTTACGCCAATTAGCCGATACCATCACGGATTTCCCTCTTGCCATTCAGCGCCTGACTTTGGCgg                                                  <  1:726378/250‑1 (MQ=255)
                                                                                                                                                                                                  ggAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAACGCAGGATCCGACgg                                                                                                                                      <  1:827855/155‑1 (MQ=255)
                                                                                                                                                                                                    aaaaTGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAACGCAGGATCCGACGGTGTTTGAACACGATGACTTTTTACGCCAATTAGCCGATACCATCACGGATTTCCCTCTTGCCATTCAGCGCCTGACTTTGGCGGAACAAAACATGGa                                     <  1:640702/250‑1 (MQ=255)
                                                                                                                                                                                                       aTGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAACGCAGGATCCGACGGTGTTTGAACACGATGACTTTTTACGCCAATTAGCCGATACCATCACGGATTTCCCTCTTGCCATTCAGCGCCTGACTTTGGCGGAACAAAACATGGATTTg                                 >  1:194910/1‑251 (MQ=255)
                                                                                                                                                                                                         gCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAACGCAGGATCCGACGGTGTTTGAACACGATGACTTTTTACGCCAATTAGCCGATACCATCACGGATTTCCCTCTTGCCATTCAGCGCCTGACTTTGGCGGAACAAAACATGGATTTgg                                >  1:36828/1‑250 (MQ=255)
                                                                                                                                                                                                            tttCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAACGCAGGATCCGACGGTGTTTGAACACGATGACTTTTTACGCCAATTAGCCGATACCATCACGGATTTCCCTCTTGCCATTCAGCGCCTGACTTTGGCGGAACAAAACATGGATTTggcgg                             >  1:566729/1‑250 (MQ=255)
                                                                                                                                                                                                             ttCCTCATGTGTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAACGCAGGATCCGACGGTGTTTGAACACGATGACTTTTTACGCCAATTAGCCGATACCATCACGGATTTCCCTCTTGCCATTCAGCGCCTGACTTTGGCGGAACAAAACATGGATTTGgcggcg                           >  1:410333/1‑251 (MQ=255)
                                                                                                                                                                                                                    tgtgTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGaaa                                                                                                                                                                                                               >  1:247261/1‑64 (MQ=255)
                                                                                                                                                                                                                    tgtgTTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATcaaca                                                                                                                                                       >  1:274914/1‑120 (MQ=255)
                                                                                                                                                                                                                      tgtTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAACGCAGGATCCGACGGTGTTTGAACACGATGACTTTTTACGCCAATTAGCCGATACCATCACGGATTTCCCTCTTGCCATTCAGCGCCTGACTTTGGCGGAACAAAACATGGATTTGGCGGCGATTTATAc                   <  1:746325/250‑1 (MQ=255)
                                                                                                                                                                                                                      tgtTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAACGCAGGATCCGACGGTGTTTGAACACGATGACTTTTTACGCCAATTAGCCGATACCATCACGGATTTCCCTCTTGCCATTCAGCGCCTGACTTTGGCGGAACAAAACATGGATTTGGCGGCGATTTATAc                   <  1:704277/250‑1 (MQ=255)
                                                                                                                                                                                                                      tgtTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAACGCAGGATCCGACGGTGTTTGAACACGATGACTTTTTACGCCAATTAGCCGATACCATCACGGATTTCCCTCTTGCCATTCAGCGCCTGACTTTGGCGGAACAAAACATGGATTTGGCGGCGATTTATAc                   <  1:672679/250‑1 (MQ=255)
                                                                                                                                                                                                                      tgtTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAACGCAGGATCCGACGGTGTTTGAACACGATGACTTTTTACGCCAATTAGCCGATACCATCACGGATTTCCCTCTTGCCATTCAGCGCCTGACTTTGGCGGAACAAAACATGGATTTGGCGGCGATTTATAc                   >  1:57117/1‑250 (MQ=255)
                                                                                                                                                                                                                      tgtTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAACGCAAGATCCGACGGTGTTTGAACACGATGACTTTTTACGCCAATTAGCCGATACCATCACGGATTTCCCTCTTGCCATTCAGCGCCTGACTTTGGCGGAACAAAACATGGATTTGGCGGCGATTTATAc                   <  1:9077/250‑1 (MQ=255)
                                                                                                                                                                                                                       gtTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAACGCAGGATCCGACGGTGTTTGAACac                                                                                                                           >  1:393128/1‑144 (MQ=255)
                                                                                                                                                                                                                       gtTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAACGCAGGATCCGACGGTGTTTGAACACGATGACTTTTTACGCCAATTAGCCGATACCATCACGGATTTCCCTCTTGCCATTCAGCGCCTGACTTTGGCGGAACAAAACATGGa                                     >  1:139088/1‑231 (MQ=255)
                                                                                                                                                                                                                       gtTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAACGCAGGATCCGACGGTGTTTGAACACGATGACTTTTTACGCCAATTAGCCGATACCATCACGGATTTCCCTCTTGCCATTCAGCGCCTGACTTTGGCGGAACAAAACATGGATTTggc                               >  1:675499/1‑237 (MQ=255)
                                                                                                                                                                                                                       gtTAAATGTGGAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAACGCAGGATCCGACGGTGTTTGAACACGATGACTTTTTACGCCAATTAGCCGATACCATCACGGATTTCCCTCTTGCCATTCAGCGCCTGACTTTGGCGGAACAAAACATGGATTTGGCGGCGATTTATACCa                 >  1:810357/1‑251 (MQ=255)
                                                                                                                                                                                                                                 gAGCAAATTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAACGCAGGATCCGACGGTGTTTGAACACGATGACTTTTTACGCCAATTAGCCGATACCATCACGGATTTCCCTCTTGCCATTCAGCGCCTGACTTTGGCGGAACAAAACATGGATTTGGCGGCGATTTATACCATTCATGGAt        <  1:282174/250‑1 (MQ=255)
                                                                                                                                                                                                                                     aaaTTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAACGCAGGATCCGACGGTGTTTGAACACGATGACTTTTTACGCCAATTAGCCGATACCATCACGGATTTCCCTCTTGCCATTCAGCGCCTGACTTTGGCGGAACAAAACATGGATTTGGCGGCGATTTATACCATTCATGGATTTTg    >  1:212854/1‑250 (MQ=255)
                                                                                                                                                                                                                                      aaTTTTGGTGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAACGCAGGATCCGACGGTGTTTGAACACGATGACTTTTTACGCCAATTAGCCGATACCATCACGGATTTCCCTCTTGCCATTCAGCGCCTGACTTTGGCGGAACAAAACATGGATTTGGCGGCGATTTATACCATTCATGGATTTTGcc  <  1:879012/251‑1 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                  
AATGAGGATATTGGGCGGAGCGGGTTTCCGCCCTTGTTTTATTCAATATTTTATTTTTATTCACCGGTAACCATGCAAGCACTCAATCCGATTTCCATTCCGTTAAACGCCATCAGTCTTATTGAAGCCTCTGCAGGGACGGGCAAAACCTACACCATGGGTTCTCTTTATTTGCGTCTGTTGTTGCAGGCGGGGGAAAATGCCTTTCCTCATGTGTTAAATGTGGAGCAAATTTTAGCGGTGACCTTTACCGAAATGGCAACGGAGGAACTGAAACGTAAAATTCGCGAGCGGATTTATGACGCCAAACAAAAATTGACGGCGTATCAACAAACGCAGGATCCGACGGTGTTTGAACAAGATGACTTTTTACGCCAATTAGCCGATACCATCACGGATTTCCCTCTTGCCATTCAGCGCCTGACTTTGGCGGAACAAAACATGGATTTGGCGGCGATTTATACCATTCATGGATTTTGCC  >  1/1160100‑1160580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑