Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,152,167	A→C	intergenic (+15118/‑7337)	unknown → / → unknown	LSU rRNA ## 23S rRNA, large subunit ribosomal RNA/tRNA‑Val‑GAC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,152,167	0	A	C	100.0%	63.3 / NA	23	intergenic (+15118/‑7337)	unknown/unknown	LSU rRNA ## 23S rRNA, large subunit ribosomal RNA/tRNA‑Val‑GAC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (8/15);  total (8/15)

TTGGCAGCAGTGTTTGGATTAACGAGTTAAAAAAACAATTACAGAAGCTGGCCAATTCGAATCTACCGGTTTTTATTTGGGGAGAGATTGGCACCGGTCGTCATTTAGCGGCTACTTATCTGCATAAATTAAGCGATCGAAAAACACTACCGATGGTTTTTCATGAATGTACACAAAATGATAATATTAATATTATTGAGCTAATTAAAGAAAAAAAATCAGGGACTTTAATTATTAAGAATGTGCATTATCTTCCTGTTGAAGAACAACAATATTTAATTCAGATTTTACATCATGACGAGAGTCAATTCCGTTTAATTTTGCTTAGTGATATAGAACTTAACGAATTAATTCGCTGTCGGCAGATAAGTGCCGAGTTTTATTATTTTTTTATTTATACTCAAATTGAAATGCTGCCGTTACGTAAACATATCACTGATGTGGCAGATATTT  >  1/1151954‑1152406                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                ttGGCAGCAGTGTTTGGATTAACGAGTTAAAAAAACAATTACAGAAGCTGGCCAATTCGAATCTACCGGTTTTTATTTGGGGAGAGATTGGCACCGGTCGTCATTTAGCGGCTACTTATCTGCATAAATTAAGCGATCGAAAAACACTACCGATGGTTTTTCATGAATGTACACAAAATGATAATATTAATATTATTGAGCTAATTAAAGAAACAAAATCAGGGACTTTAATTATTAAGAATGTGCATTAt                                                                                                                                                                                                            <  1:816438/251‑1 (MQ=255)       gcagTGTTTGGATTAACGAGTTAAAAAAACAATTACAGAAGCTGGCCAATTCGAATCTACCGGTTTTTATTTGGGGAGAGATTGGCACCGGTCGTCATTTAGCGGCTACTTATCTGCATAAATTAAGCGATCGAAAAACACTACCGATGGTTTTTCATGAATGTACACAAAATGATAATATTAATATTATTGAGCTAATTAAAGAAACAAAATCAGGGACTTTAATTATTAAGAATGTGCATTATCTTcc                                                                                                                                                                                                       >  1:152808/1‑250 (MQ=255)                   ttAACGAGTTAAAAAAACAATTACAGAAGCTGGCCAATTCGAATCTACCGGTTTTTATTTGGGGAGAGATTGGCACCGGTCGTCATTTAGCGGCTACTTATCTGCATAAATTAAGCGATCGAAAAACACTACCGATGGTTTTTCATGAATGTACACAAAATGATAATATTAATATTATTGAGCTAATTAAAGAAACAAAATCAGGGACTTTAATTATTAAGAATGTGCATTATCTTCCTGTTGAAGaacaa                                                                                                                                                                                          <  1:437030/251‑1 (MQ=255)                                  aaCAATTACAGAAGCTGGCCAATTCGAATCTACCGGTTTTTATTTGGGGAGAGATTGGCACCGGTCGTCATTTAGCGGCTACTTATCTGCATAAATTAAGCGATCGAAAAACACTACCGATGGTTTTTCATGAATGTACACAAAATGATAATATTAATATTATTGAGCTAATTAAAGAAACAAAATCAGGGACTTTAATTATTAAGAATGTGCa                                                                                                                                                                                                                <  1:1020/214‑1 (MQ=255)                                                                     gTTTTTATTTGGGGAGAGATTGGCACCGGTCGTCATTTAGCGGCTACTTATCTGCATAAATTAAGCGATCGAAAAACACTACCGATGGTTTTTCATGAATGTACACAAAATGATAATATTAATATTATTGAGCTAATTAAAGAAACAAAATCAGGGACTTTAATTATTAAGAATGTGCATTATCTTCCTGTTGAAGAACAACAATATTTAATTCAGATTTTACATCATGACGAGAGTCAATTc                                                                                                                                                >  1:149387/1‑243 (MQ=255)                                                                                      gaTTGGCACCGGTCGTCATTTAGCGGCTACTTATCTGCATAAATTAAGCGATCGAAAAACACTACCGATGGTTTTTCATGAATGTACACAAAATGATAATATTAATATTATTGAGCTAATTAAAGAAACAAAATCAGGGACTTTAATTATTAAGAATGTGCATTATc                                                                                                                                                                                                           >  1:543309/1‑167 (MQ=255)                                                                                      gaTTGGCACCGGTCGTCATTTAGCGGCTACTTATCTGCATAAATTAAGCGATCGAAAAACACTACCGATGGTTTTTCATGAATGTACACAAAATGATAATATTAATATTATTGAGCTAATTAAAGAAACAAAATCAGGGACTTTAATTATTAAGAATGTGCATTATCTTCCTGTTGAAGAACAACAATATTTAATTCAGATTTTACATCATGACGAGAGTCAATTCCGTTTAATTTTGCTTAGTGATATAg                                                                                                                       >  1:705590/1‑251 (MQ=255)                                                                                            cACCGGTCGTCATTTAGCGGCTACTTATCTGCATAAATTAAGCGATCGAAAAACACTACCGATGGTTTTTCATGAATGTACACAAAATGATAATATTAATATTATTGAGCTAATTAAAGAAACAAAATCAGGGACTTTAATTATTAAGAATGTGCATTATCTTCCTGTTGAAGAACAACAATATTTAATTCAGATTTTACATCATGACGAGAGTCAATTCCGTTTAATTTTGCTTAGTGATATAGAACtt                                                                                                                  <  1:253250/250‑1 (MQ=255)                                                                                                                  aCTTATCTGCATAAATTAAGCGATCGAAAAACACTACCGATGGTTTTTCATGAATGTACACAAAATGATAATATTAATATTATTGAGCTAATTAAAGAAACAAAATCAGGGACTTTAATTATTAAGAATGTGCATTATc                                                                                                                                                                                                           <  1:299606/139‑1 (MQ=255)                                                                                                                        cTGCATAAATTAAGCGATCGAAAAACACTACCGATGGTTTTTCATGAATGTACACAAAATGATAATATTAATATTATTGAGCTAATTAAAGAAACAAAATCAGGGACTTTAATTATTAAGAATGTGCATTATCTTCCTGTTGAAGAACAACAATATTTAATTCAGATTTTACATCATGACGAGAGTCAATTCCGTTTAATTTTGCTTAGTGATATAGAACTTAACGAATTAATTCGCTGTCGGCAGATAAg                                                                                     <  1:814587/251‑1 (MQ=255)                                                                                                                          gCATAAATTAAGCGATCGAAAAACACTACCGATGGTTTTTCATGAATGTACACAAAATGATAATATTAATATTATTGAGCTAATTAAAGAAACAAAATCAGGGACTTTAATTATTAAGAATGTGCATTATCTTCCTGTTGAAGAACAACAATATTTAATTCAGATTTTAca                                                                                                                                                                   <  1:740536/171‑1 (MQ=255)                                                                                                                                         tCGAAAAACACTACCGATGGTTTTTCATGAATGTACACAAAATGATAATATTAATATTATTGAGCTAATTAAAGAAACAAAATCAGGGACTTTAATTATTAAGAATGTGCATTATCTTCCTGTTGAAGAACAACAATATTTAATTCAGATTTTACATCATGACGAGAGTCAATTCCGTTTAATTTTGCTTAGTGATATAGAACTTAACGAATTAATTCGCTGTCGGCAGATa                                                                                       >  1:88137/1‑232 (MQ=255)                                                                                                                                                      ccGATGGTTTTTCATGAATGTACACAAAATGATAATATTAATATTATTGAGCTAATTAAAGAAACAAAATCAGGGACTTTAATTATTAAGAATGTGCATTATCTTCCTGTTGAAGAACAACAATATTTAATTCAGATTTTACATCATGACgag                                                                                                                                                         <  1:277990/153‑1 (MQ=255)                                                                                                                                                                           acacAAAATGATAATATTAATATTATTGAGCTAATTAAAGAAACAAAATCAGGGACTTTAATTATTAAGAATGTGCATTATCTTCCTGTTGAAGAACAACAATATTTAATTCAGATTTTAc                                                                                                                                                                    <  1:193183/121‑1 (MQ=255)                                                                                                                                                                                         tattaatattaTTGAGCTAATTAAAGAAACAAAATCAGGGACTTTAATTATTAAGAATGTGCATTATCTTCCTGTTGAAGaac                                                                                                                                                                                            <  1:587179/83‑1 (MQ=255)                                                                                                                                                                                              atattaTTGAGCTAATTAAAGAAACAAAATCAGGGACTTTAATTATTAAGAATGTGCATTATCt                                                                                                                                                                                                          <  1:781965/64‑1 (MQ=255)                                                                                                                                                                                              atattaTTGAGCTAATTAAAGAAACAAAATCAGGGACTTTAATTATTAAGAATGTGCATTATCTTCCTGTTGAAGAACAACAATATTTAATTCAGATTTTACATCATGACGAGAGTCAATTCCGTTTAATTTTGCTTAGTGATATAGAACTTAACGAATTAATTCGCTGTCGGCAGATAAGTGCCGAGTTTTATTATTTTTTTATTTATACTCAAATTGAAATGCTGCCGTTACGTAAACATATCACTGAt               >  1:789096/1‑251 (MQ=255)                                                                                                                                                                                                 ttattGAGCTAATTAAAGAAACAAAATCAGGGACTTTAATTATTAAGAATGTGCATTATCTTCCTGTTGAAGAACAACAATATTTAATTCAGATTTTACATCATGACGAGAGTCAATTCCGTTTAATTTTGCTTAGTGATATAGAAc                                                                                                                    <  1:731207/147‑1 (MQ=255)                                                                                                                                                                                                 ttattGAGCTAATTAAAGAAACAAAATCAGGGACTTTAATTATTAAGAATGTGCATTATCTTCCTGTTGAAGAACAACAATATTTAATTCAGATTTTACATCATGACGAGAGTCAATTCCGTTTAATTTTGCTTAGTGATATAGAACTTAACGAATTAATTCGCTGTCGGCAGATAAGTGCCGAGTTTTATTATTTTTTTATTTATACTCAAATTGAAATGCTGCCGTTACGTAAACa                         <  1:763249/238‑1 (MQ=255)                                                                                                                                                                                                        gCTAATTAAAGAAACAAAATCAGGGACTTTAATTATTAAGAATGTGCATTATCTTCCTGTTGAAGAACAACAATATTTAATTCAGATTTTACATCATGACGAGAGTCAATTCCGTTTAATTTTGCTTAGTGATATAGAACTTAACGAATTAATTCGCTGTCGGCAGATAAGTGCCGAGTTTTATTATTTTTTTATTTATACTCAAATTGAAATGCTGCCGTTACGTAAACATATCACTGAtg              <  1:369515/242‑1 (MQ=255)                                                                                                                                                                                                           aaTTAAAGAAACAAAATCAGGGACTTTAATTATTAAGAATGTGCATTATCTTCCTGTTGAAGAACAACAATATTTAATTCAGATTTTACATCATGACGAGAGTCAATTCCGTTTAATTTTGCTTAGTGATATAg                                                                                                                       >  1:415667/1‑134 (MQ=255)                                                                                                                                                                                                           aaTTAAAGAAACAAAATCAGGGACTTTAATTATTAAGAATGTGCATTATCTTCCTGTTGAAGAACAACAATATTTAATTCAGATTTTACATCATGACGAGAGTCAATTCCGTTTAATTTTGCTTAGTGATATAGAACTTAACGAATTAATTc                                                                                                     <  1:274912/152‑1 (MQ=255)                                                                                                                                                                                                           aaTTAAAGAAACAAAATCAGGGACTTTAATTATTAAGAATGTGCATTATCTTCCTGTTGAAGAACAACAATATTTAATTCAGATTTTACATCATGACGAGAGTCAATTCCGTTTAATTTTGCTTAGTGATATAGAACTTAACGAATTAATTCGCTGTCGGCAGATAAGTGCCGAGTTTTATTATTTTTTTATTTATACTCAAATTGAAATGCTGCCGTTACGTAAACATATCACTGATGTGGCAGATAttt  >  1:15592/1‑251 (MQ=255)                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                TTGGCAGCAGTGTTTGGATTAACGAGTTAAAAAAACAATTACAGAAGCTGGCCAATTCGAATCTACCGGTTTTTATTTGGGGAGAGATTGGCACCGGTCGTCATTTAGCGGCTACTTATCTGCATAAATTAAGCGATCGAAAAACACTACCGATGGTTTTTCATGAATGTACACAAAATGATAATATTAATATTATTGAGCTAATTAAAGAAAAAAAATCAGGGACTTTAATTATTAAGAATGTGCATTATCTTCCTGTTGAAGAACAACAATATTTAATTCAGATTTTACATCATGACGAGAGTCAATTCCGTTTAATTTTGCTTAGTGATATAGAACTTAACGAATTAATTCGCTGTCGGCAGATAAGTGCCGAGTTTTATTATTTTTTTATTTATACTCAAATTGAAATGCTGCCGTTACGTAAACATATCACTGATGTGGCAGATATTT  >  1/1151954‑1152406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑