Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,151,665	G→A	intergenic (+14616/‑7839)	unknown → / → unknown	LSU rRNA ## 23S rRNA, large subunit ribosomal RNA/tRNA‑Val‑GAC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,151,665	0	G	A	100.0%	95.7 / NA	37	intergenic (+14616/‑7839)	unknown/unknown	LSU rRNA ## 23S rRNA, large subunit ribosomal RNA/tRNA‑Val‑GAC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/18);  total (19/18)

ATCAATAAGTCTATCCATTATGCAACAATGTCAAGGAAACTTATTAATTGCTTCTACATTGACAAAAAATGCGCTGGTTATTTTGGAATTTAAGGTGGTAGATAATGTTTAATTATCAGAATAATGTTTTATTAATTGATGATGATTTTGATATTTTGGAATCGTATGCCGATTTATTACAACAAGAAGGCTATTCTGTATTTGCAACTGCCGAACCTAAAGAGATTATTAATCAAATTCCGGAAAATTGGTATGGCGTTGTGATTTGCGACGTATTATTGCTGGGCATTTCAGGATTAGATGATTAGATCGAATGATTGAAATGGATAGCCAAATTCCGGTGATTATGATTACCGGGCATGGTGATGTTCCCATGGCGGTAGAAGCTGTCAAGAAAGGGGCGACAAATTTCTTTGAAAAACCGGTTTCCCCTGAAAAATTACTTATTCAAGTCGATAACGCTT  >  1/1151424‑1151887                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                               aTCAATAAGTCTATCCATTATGCAACAATGTCAAGGAAACTTATTAATTGCTTCTACATTGACAAAAAATACGCTGGTTATTTTGGAATTTAAGGTGGTAGATAATGTTTAATTATCAGAATAATGTTTTATTAATTGATGATGATTTTGATATTTTGGAATCGTATGCCGATTTATTACAACAAGAAGGCTATTCTGTATTTGCAACTGCCGAACCTAAAGAGATTATTAATCAAATTCCAGAAAATTgg                                                                                                                                                                                                                       >  1:103449/1‑251 (MQ=255)        aGTCTATCCATTATGCAACAATGTCAAGGAAACTTATTAATTGCTTCTACATTGACAAAAAATACGCTGGTTATTTTGGAATTTAAGGTGGTAGATAATGTTTAATTATCAGAATAATGTTTTATTAATTGATGATGATTTTGATATTTTGGAATCGTATGCCGATTTATTACAACAAGAAGGCTATTCTGTATTTGCAACTGCCGAACCTAAAGAGATTATTAATCAAATTCCAGAAAATTGGTATGGCg                                                                                                                                                                                                                >  1:837162/1‑251 (MQ=255)                            aTGTCAAGGAAACTTATTAATTGCTTCTACATTGACAAAAAATACGCTGGTTATTTTGGAATTTAAGGTGGTAGATAATGTTTAATTATCAGAATAATGTTTTATTAATTGATGATGATTTTGATATTTTGGAATCGTATGCCGATTTATTACAACAAGAAGGCTATTCTGTATTTGCAACTGCCGAACCTAAAGAGATTATTAATCAAATTCCAGAAAATTGGTATGGCGTTGTGATTTGCGACGtatta                                                                                                                                                                                            >  1:261453/1‑251 (MQ=255)                            aTGTCAAGGAAACTTATTAATTGCTTCTACATTGACAAAAAATACGCTGGTTATTTTGGAATTTAAGGTGGTAGATAATGTTTAATTATCAGAATAATGTTTTATTAATTGATGATGATTTTGATATTTTGGAATCGTATGCCGATTTATTACAACAAGAAGGCTATTCTGTATTTGCAACTGCCGAACCTAAAGAGATTATTAATCAAATTCCAGAAAATTGGTATGGCGTTGTGATTTGCGACGtatta                                                                                                                                                                                            >  1:40952/1‑251 (MQ=255)                               tCAAGGAAACTTATTAATTGCTTCTACATTGACAAAAAATACGCTGGTTATTTTGGAATTTAAGGTGGTAGATAATGTTTAATTATCAGAATAATGTTTTATTAATTGATGATGATTTTGATATTTTGGAATCGTATGCCGATTTATTACAACAAGAAGGCTATTCTGTATTTGCAACTGCCGAACCTAAAGAGATTATTAATCAAATTCCAGAAAATTGGTATGGCGTTGTGATTTGCGACGTATTATTg                                                                                                                                                                                         <  1:578002/251‑1 (MQ=255)                                       aCTTATTAATTGCTTCTACATTGACAAAAAATACGCTGGTTATTTTGGAATTTAAGGTGGTAGATAATGTTTAATTATCAGAATAATGTTTTATTAATTGATGATGATTTTGATATTTTGGAATCGTATGCCGATTTATTACAACAAGAAGGCTATTCTGTATTTGCAACTGCCGAACCTAAAGAGATTATTAATCAAATTCCAGAAAAt                                                                                                                                                                                                                          >  1:178215/1‑210 (MQ=255)                                          tattaATTGCTTCTACATTGACAAAAAATACGCTGGTTATTTTGGAATTTAAGGTGGTAGATAATGTTTAATTATCAGAATAATGTTTTATTAATTGATGATGATTTTGATATTTTGGAATCGTATGCCGATTTATTACAACAAGAAGGCTATTCTGTATTTGCAACTGCCGAACCTAAAGAGATTATTAATCAAATTCCAGAAAATTGGTATGGCGTTGTGa                                                                                                                                                                                                          >  1:763912/1‑223 (MQ=255)                                                   cTTCTACATTGACAAAAAATACGCTGGTTATTTTGGAATTTAAGGTGGTAGATAATGTTTAATTATCAGAATAATGTTTTATTAATTGATGATGATTTTGATATTTTGGAATCGTATGCCGATTTATTACAACAAGAAGGCTATTCTGTATTTGCAACTGCCGAACCTAAAGAGATTATTAATCAAATTCCAGAAAATTGGTATGGCGTTGTGATTTGCGACGTATTATTGCTGGGCATTTCAGgattaga                                                                                                                                                                     >  1:196323/1‑251 (MQ=255)                                                    ttCTACATTGACAAAAAATACGCTGGTTATTTTGGAATTTAAGGTGGTAGATAATGTTTAATTATCAGAATAATGTTTTATTAATTGATGATGATTTTGATATTTTGGAATCGTATGCCGATTTATTACAACAAGAAGGCTATTCTGTATTTGCAACTGCCGAACCTAAAGAGATTATTAATCAAATTCCAGAAAATTGGTATGGCGTTGTGATTTGCGACGtattat                                                                                                                                                                                           >  1:737832/1‑228 (MQ=255)                                                                                              ggtggtAGATAATGTTTAATTATCAGAATAATGTTTTATTAATTGATGATGATTTTGATATTTTGGAATCGTATGCCGATTTATTACAACAAGAAGGCTATTCTGTATTTGCAACTGCCGAACCTAAAGAGATTATTAATCAAATTCCAGAAAATTGGTATGGCGTTGTGATTTGCGACGTATTATTGc                                                                                                                                                                                        <  1:673823/189‑1 (MQ=255)                                                                                               gtggtAGATAATGTTTAATTATCAGAATAATGTTTTATTAATTGATGATGATTTTGATATTTTGGAATCGTATGCCGATTTATTACAACAAGAAGGCTATTCTGTATTTGCAACTGCCGAACCTAAAGAGATTATTAATCAAATTCCAGAAAATTGGTATGGCGTTGTGATTTGCGACGTATTATTGCTGGGCATTTCAGGATTAGATGATTAGATCGAATGATTGAAATGGATAGCCAAATTCCGGtgat                                                                                                                         >  1:116524/1‑251 (MQ=255)                                                                                                     gATAATGTTTAATTATCAGAATAATGTTTTATTAATTGATGATGATTTTGATATTTTGGAATCGTATGCCGATTTATTACAACAAGAAGGCTATTCTGTATTTGCAACTGCCGAACCTAAAGAGATTATTAATCAAATTCCAGAAAATTGGTATGGCGTTGTGATTTGCGACGTATTATTGCTGGGCATTTCAGGATTAGATGATTAGATCGAATGATTGAAATGGATAGCCAAATTCCGGtgattatga                                                                                                                    <  1:733873/250‑1 (MQ=255)                                                                                                         aTGTTTAATTATCAGAATAATGTTTTATTAATTGATGATGATTTTGATATTTTGGAATCGTATGCCGATTTATTACAACAAGAAGGCTATTCTGTATTTGCAACTGCCGAACCTAAAGAGATTATTAATCAAATTCCAGAAAATTGGTATGGCGTTGTGATTTGCGa                                                                                                                                                                                                   <  1:398626/167‑1 (MQ=255)                                                                                                           gTTTAATTATCAGAATAATGTTTTATTAATTGATGATGATTTTGATATTTTGGAATCGTATGCCGATTTATTACAACAAGAAGGCTATTCTGTATTTGCAACTGCCGAACCTAAAGAGATTATTAATCAAATTCCAGAAAATTGGTATGGCGTTGTGATTTGCGACGTATTATTGCTGGGCATTTCAGGATTAGATGATTAGATCGAATGATTGAAATGGATAGCCAAATTCCGGTGATTATGATTACCgg                                                                                                             >  1:641197/1‑251 (MQ=255)                                                                                                             ttAATTATCAGAATAATGTTTTATTAATTGATGATGATTTTGATATTTTGGAATCGTATGCCGATTTATTACAACAAGAAGGCTATTCTGTATTTGCAACTGCCGAACCTAAAGAGATTATTAATCAAATTCCAGAAAATTGGTATGGCGTTGTGATTTGCGACGTATTATTGCTGGGCATTTCAGGATTAGATGATTAGATCGAATGATTGAAATGGATAGCCAAATTCCGGTGATTATGATTACCGGGc                                                                                                           <  1:862093/251‑1 (MQ=255)                                                                                                                              gTTTTATTAATTGATGATGATTTTGATATTTTGGAATCGTATGCCGATTTATTACAACAAGAAGGCTATTCTGTATTTGCAACTGCCGAACCTAAAGAGATTATTAATCAAATTCCAGAAAATTGGTATGGCGTTGTGATTTGCGACGTATTATTGCTGGGCATTTCAGgattagatgattagat                                                                                                                                                            <  1:900887/185‑1 (MQ=255)                                                                                                                              gTTTTATTAATTGATGATGATTTTGATATTTTGGAATCGTATGCCGATTTATTACAACAAGAAGGCTATTCTGTATTTGCAACTGCCGAACCTAAAGAGATTATTAATCAAATTCCAGAAAATTGGTATGGCGTTGTGATTTGCGACGTATTATTGCTGGGCATTTCAGGATTAGATGATTAGATCGAATGATTGAAATGGATAGCCAAATTCCGGTGATTATGATTACCGGGCATGGTGATGTTc                                                                                               <  1:251361/246‑1 (MQ=255)                                                                                                                              gTTTTATTAATTGATGATGATTTTGATATTTTGGAATCGTATGCCGATTTATTACAACAAGAAGGCTATTCTGGATTTGCAACTGCCGAACCTAAAGAGATTATTAATCAAATTCCAGAAAATTGGTATGGCGTTGTGATTTGCGACGTATTATTGCTGGGCATTTCAGGATTAGATGATTAGATCGAATGATTGAAATGGATAGCCAAATTCCGGTGATTATGATTACCGGGCATGGTGATg                                                                                                  <  1:449481/243‑1 (MQ=255)                                                                                                                                   attaATTGATGATGATTTTGATATTTTGGAATCGTATGCCGATTTATTACAACAAGAAGGCTATTCTGTATTTGCAACTGCCGAACCTAAAGAGATTATTAATCAAATTCCAGAAAATTGGTATGGCGTTGTGATTTGCGACGTATTATTGCTGGGCATTTCAGGATTAGATGATTAGATCGAATGATTGAAATGGATAGCCAAATTCCGGtgattatg                                                                                                                     >  1:201812/1‑219 (MQ=255)                                                                                                                                                  ttttGATATTTTGGAATCGTATGCCGATTTATTACAACAAGAAGGCTATTCTGTATTTGCAACTGCCGAACCTAAAGAGATTATTAATCAAATTCCAGAAAATTGGTATGGCGTTGTGATTTGCGa                                                                                                                                                                                                   <  1:159826/126‑1 (MQ=255)                                                                                                                                                  ttttGATATTTTGGAATCGTATGCCGATTTATTACAACAAGAAGGCTATTCTGTATTTGCAACTGCCGAACCTAAAGAGATTATTAATCAAATTCCAGAAAATTGGTATGGCGTTGTGATTTGCGACGTATTATTGCTGGGCATTTCAGgattagatgattagat                                                                                                                                                            <  1:546426/165‑1 (MQ=255)                                                                                                                                                     tGATATTTTGGAATCGTATGCCGATTTATTACAACAAGAAGGCTATTCTGTATTTGCAACTGCCGAACCTAAAGAGATTATTAATCAAATTCCAGAAAATTGGTATGGCGTTGTGATTTGCGACGTATTATTGCTGGGCATTTCAGGATTAGATGATTAGATCGAATGATTGAAATGGATAGCCAAATTCCGGTGATTATGATTACCGGGCATGGTGATGTTCCCATGGCGGTAGAAGCTGTCAAGAAAgg                                                                   <  1:809233/251‑1 (MQ=255)                                                                                                                                                          ttttGGAATCGTATGCCGATTTATTACAACAAGAAGGCTATTCTGTATTTGCAACTGCCGAACCTAAAGAGATTATTAATCAAATTCCAGAAAATTGGTATGGCGTTGTGATTTGCGACGTATTATTGCTGGGCATTTCAGGATTAGATGATTAGATCGAATGATTGAAATGGATAGCCAAATTCCGGTGATTATGATTACCGGGCATGGTGATGTTCCCATgg                                                                                         <  1:336973/224‑1 (MQ=255)                                                                                                                                                                    gTATGCCGATTTATTACAACAAGAAGGCTATTCTGTATTTGCAACTGCCTAACCTAAAGAGATTATTAATCAAATTCCAGAAAATTGGTATGGCGTTGTGATTTGCGACGTATTATTGCTGGGCATTTCAGGATTAGATGATTAGATCGAATGATTGAAATGGATAGCCAAATTCCGGTGATTATGATTACCGGGCATGGTGATGTTc                                                                                               <  1:710679/208‑1 (MQ=255)                                                                                                                                                                        gCCGATTTATTACAACAAGAAGGCTATTCTGTATTTGCAACTGCCGAACCTAAAGAGATTATTAATCAAATTCCAGAAAATTGGTATGGCGTTGTGATTTGCGACGTATTATTGCTGGGCAt                                                                                                                                                                                 >  1:520114/1‑122 (MQ=255)                                                                                                                                                                                       caaGAAGGCTATTCTGTATTTGCAACTGCCGAACCTAAAGAGATTATTAATCAAATTCCAGAAAATTGGTATGGCGTTGTGATTTGCGACGTATTATTGCTGGGCATTTCAGGATTAGATGATTAGATCGAATGATTGAAATGGAt                                                                                                                                          >  1:780717/1‑146 (MQ=255)                                                                                                                                                                                       caaGAAGGCTATTCTGTATTTGCAACTGCCGAACCTAAAGAGATTATTAATCAAATTCCAGAAAATTGGTATGGCGTTGTGATTTGCGACGTATTATTGCTGGGCATTTCAGGATTAGATGATTAGATCGAATGATTGAAATGGATAGCCAAATTCCGGTGATTATGATTACCGGGCATGGTGATGTTCCCATGGCGGTAGAAGCTGTCAAGAAAGGGGCGACAAATTTCTTTGAAAAACCGGTTTccc                                   >  1:806412/1‑249 (MQ=255)                                                                                                                                                                                        aagaagGCTATTCTGTATTTGCAACTGCCGAACCTAAAGAGATTATTAATCAAATTCCAGAAAATTGGTATGGCGTTGTGATTTGCGACGTATTATTGCTGGGCATTTCAGGATTAGATGATTAGATCGAATGATTGAAATGGATAGCCAAATTCCGGTGATTATGATTACCGGGCATGGTGATGTTCCCATGGCGGTAGAAGCTGTCAAGAAAGGGGCGACAAATTTCTTTGAAAAACCGGTTTccc                                   >  1:422623/1‑248 (MQ=255)                                                                                                                                                                                            agGCTATTCTGTATTTGCAACTGCCGAACCTAAAGAGATTATTAATCAAATTCCAGAAAATTGGTATGGCGTTGTGATTTGCGACGTATTATTGCTGGGCATTTCAGGATTAGATGATTAGATCGAATGATTGAAATGGATAGCCAAATTCCGGtgattatgat                                                                                                                   <  1:834136/164‑1 (MQ=255)                                                                                                                                                                                             ggCTATTCTGTATTTGCAACTGCCGAACCTAAAGAGATTATTAATCAAATTCCAGAAAATTGGTATGGCGTTGTGATTTGCGACGTATTATTGCTGGGCATTTCAGGATTAGATGATTAGATCGAATGATTGAAATGGATAGCCAAATTCCGGTGATTATGATTACCGGGCATGGTGATGTTCCCATGGCGGTAGAAGCTGTCAAGAAAGGGGCGACAAATTTCTTTGAAAAACCGGTTTCCCCTGaaaaa                           >  1:356504/1‑251 (MQ=255)                                                                                                                                                                                              gCTATTCTGTATTTGCAACTGCCGAACCTAAAGAGATTATTAATCAAATTCCAGAAAATTgg                                                                                                                                                                                                                       <  1:788689/62‑1 (MQ=255)                                                                                                                                                                                                      gTATTTGCAACTGCCGAACCTAAAGAGATTATTAATCAAATTCCAGAAAATTGGTATGGCGTTGTGATTTGCGACGTATTATTGCTGGGCATTTCAGGATTAGATGATTAGATCGAATGATTGAAATGGATAGCCAAATTCCGGTGATTATGATTACCGGGCATGGTGATGTTCCCATGGCGGTAGAAGCTGTCAAGaaa                                                                     >  1:662463/1‑200 (MQ=255)                                                                                                                                                                                                         tttGCAACTGCCGAACCTAAAGAGATTATTAATCAAATTCCAGAAAATTGGTATGGCGTTGTGATTTGCGACGTATTATTGc                                                                                                                                                                                        <  1:525520/82‑1 (MQ=255)                                                                                                                                                                                                                 tGCCGAACCTAAAGAGATTATTAATCAAATTCCAGAAAATTGGTATGGCGTTGTGATTTGCGACGTATTATTGCTGGGCATTTCAGGATTAGATGATTAGATCGAATGATTGAAATGGATAGCCAAATTCCGGTGATTATGATTACCGGGCATGGTGATGTTCCCATGGCGGTAGAAGCTGTCAAGAAAGGGGCGACAAATTTCTTTGAAAAACCGGTTTCCCCTGAAAAATTAc                       <  1:374872/235‑1 (MQ=255)                                                                                                                                                                                                                      aaCCTAAAGAGATTATTAATCAAATTCCAGAAAATTGGTATGGCGTTGTGATTTGCGACGTATTATTGCTGGGCATTTCAGGATTAGATGATTAGATCGAATGATTGAAATGGATAGCCAAATTCCGGTGATTATGATTACCGGGCATGGTGATGTTCCCATGGCGGTAGAAGCTGTCAAGAAAGGGGCGACAAATTTCTTTGAAAAACCGGTTTCCCCTGAAAAATTACTTATTCAAGTCGATAACGCtt  >  1:384619/1‑251 (MQ=255)                                                                                                                                                                                                                       aCCTAAAGAGATTATTAATCAAATTCCAGAAAATTGGTATGGCGTTGTGATTTGCGACGTATTATTGCTGGGCATTTCAGGATTAGATGATTAGATCGAATGATTGAAATGGATAGCCAAATTCCGGtgattatgat                                                                                                                   >  1:47022/1‑137 (MQ=255)                                                                                                                                                                                                                        ccTAAAGAGATTATTAATCAAATTCCAGAAAATTGGTATGGCGTTGTGATTTGCGACGTATTATTGCTGGGCATTTCAGGATTAGATGATTAGATCGAATGATTGAAATGGATAGCCAAATTCCGGTGATTATGATTACCGGGc                                                                                                           <  1:175362/144‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                               ATCAATAAGTCTATCCATTATGCAACAATGTCAAGGAAACTTATTAATTGCTTCTACATTGACAAAAAATGCGCTGGTTATTTTGGAATTTAAGGTGGTAGATAATGTTTAATTATCAGAATAATGTTTTATTAATTGATGATGATTTTGATATTTTGGAATCGTATGCCGATTTATTACAACAAGAAGGCTATTCTGTATTTGCAACTGCCGAACCTAAAGAGATTATTAATCAAATTCCGGAAAATTGGTATGGCGTTGTGATTTGCGACGTATTATTGCTGGGCATTTCAGGATTAGATGATTAGATCGAATGATTGAAATGGATAGCCAAATTCCGGTGATTATGATTACCGGGCATGGTGATGTTCCCATGGCGGTAGAAGCTGTCAAGAAAGGGGCGACAAATTTCTTTGAAAAACCGGTTTCCCCTGAAAAATTACTTATTCAAGTCGATAACGCTT  >  1/1151424‑1151887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑