Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,151,494	G→A	intergenic (+14445/‑8010)	unknown → / → unknown	LSU rRNA ## 23S rRNA, large subunit ribosomal RNA/tRNA‑Val‑GAC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,151,494	0	G	A	100.0%	107.7 / NA	41	intergenic (+14445/‑8010)	unknown/unknown	LSU rRNA ## 23S rRNA, large subunit ribosomal RNA/tRNA‑Val‑GAC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/12);  total (29/12)

CACTGGAAGCTATGCAGCATGAAAAACCGGAGATTGAGATAAAAATACAAAATATATCGGATCAGAGAGTAGAATTATGGGTTTCTGATAATGGTTCCGGTTGGCCGTTGTCAGATAAATTACTACAACCTTTCTCCAGTAGTAAATCCATTAATTTAGGCTTAGGATTATCAATAAGTCTATCCATTATGCAACAATGTCAAGGAAACTTATTAATTGCTTCTACATTGACAAAAAATGCGCTGGTTATTTTGGAATTTAAGGTGGTAGATAATGTTTAATTATCAGAATAATGTTTTATTAATTGATGATGATTTTGATATTTTGGAATCGTATGCCGATTTATTACAACAAGAAGGCTATTCTGTATTTGCAACTGCCGAACCTAAAGAGATTATTAATCAAATTCCGGAAAATTGGTATGGCGTTGTGATTTGCGACGTATTATTGCTGGGCATTTCAGGATTAGA  >  1/1151255‑1151724                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                       cACTGGAAGCTATGCAGCATGAAAAACCGGAGATTGAGATAAAAATACAAAATATATCGGATCAGAGAGTAGAATTATGGGTTTCTGATAATGGTTCCGGTTGGCCGTTGTCAGATAAATTACTACAACCTTTCTCCAGTAGTAAATCCATTAATTTAGGCTTAGGATTATCAATAAGTCTATCCATTATGCAACAATGTCAAGGAAACTTATTAATTGCTTCTACATTGACAAAAAATACGCTGGTTAtt                                                                                                                                                                                                                             >  1:240723/1‑251 (MQ=255)       aaGCTATGCAGCATGAAAAACCGGAGATTGAGATAAAAATACAAAATATATCGGATCAGAGAGTAGAATTATGGGTTTCTGATAATGGTTCCGGTTGGCCGTTGTCAGATAAATTACTACAACCTTTCTCCAGTAGTAAATCCATTAATTTAGGCTTAGGATTATCAATGAGTCTATCCATTATGCAACAATGTCAAGGAAACTTATTAATTGCTTCTACATTGACAAAAAATACGCTGGTTATTTTGGaa                                                                                                                                                                                                                       >  1:423417/1‑251 (MQ=255)       aaGCTATGCAGCATGAAAAACCGGAGATTGAGATAAAAATACAAAATATATCGGATCAGAGAGTAGAATTATGGGTTTCTGATAATGGTTCCGGTTGGCCGTTGTCAGATAAATTACTACAACCTTTCTCCAGTAGTAAATCCATTAATTTAGGCTTAGGATTATCAATAAGTCTATCCATTATGCAACAATGTCAAGGAAACTTATTAATTGCTTCTACATTGACAAAAAATACGCTGGtt                                                                                                                                                                                                                                <  1:841573/242‑1 (MQ=255)         gCTATGCAGCATGAAAAACCGGAGATTGAGATAAAAATACAAAATATATCGGATCAGAGAGTAGAATTATGGGTTTCTGATAATGGTTCCGGTTGGCCGTTGTCAGATAAATTACTACAACCTTTCTCCAGTAGTAAATCCATTAATTTAGGCTTAGGATTATCAATAAGTCTATCCATTATGCAACAATGTCAAGGAAACTTATTAATTGCTTCTACATTGACAAAAAATACGCTGGTTATTTTGGAAtt                                                                                                                                                                                                                     >  1:811138/1‑251 (MQ=255)                      aaaaaCCGGAGATTGAGATAAAAATACAAAATATATCGGATCAGAGAGTAGAATTATGGGTTTCTGATAATGGTTCCGGTTGGCCGTTGTCAGATAAATTACTACAACCTTTCTCCAGTAGTAAATCCATTAATTTAGGCTTAGGATTATCAATAAGTCTATCCATTATGCAACAATGTCAAGGAAACTTATTAATTGCTTCTACATTGACAAAAAATACGCTGGTTATTTTGGAATTTAAGGTGGTAGAt                                                                                                                                                                                                        <  1:470412/251‑1 (MQ=255)                          aCCGGAGATTGAGATAAAAATACAAAATATATCGGATCAGAGAGTAGAATTATGGGTTTCTGATAATGGTTCCGGTTGGCCGTTGTCAGATAAATTACTACAACCTTTCTCCAGTAGTAAATCCATTAATTTAGGCTTAGGATTATCAATAAGTCTATCCATTATGCAACAATGTCAAGGAAACTTATTAATTGCTTCTACATTGACAAAAAATACGCTGGTTATTTTGGAATTTAAGGTGGTAGATAATg                                                                                                                                                                                                    <  1:308608/251‑1 (MQ=255)                                  ttGAGATAAAAATACAAAATATATCGGATCAGAGAGTAGAATTATGGGTTTCTGATAATGGTTCCGGTTGGCCGTTGTCAGATAAATTACTACAACCTTTCTCCAGTAGTAAATCCATTAATTTAGGCTTAGGATTATCAATAAGTCTATCCATTATGCAACAATGTCAAGGAAACTTATTAATTGCTTCTACATTGACAAAAAATACGCTGGTTATTTTGGAATTTAAGGTGGTAGATAATGTTTAATTa                                                                                                                                                                                            <  1:437118/251‑1 (MQ=255)                                     agaTAAAAATACAAAATATATCGGATCAGAGAGTAGAATTATGGGTTTCTGATAATGGTTCCGGTTGGCCGTTGTCAGATAAATTACTACAACCTTTCTCCAGTAGTAAATCCATTAATTTAGGCTTAGGATTATCAATAAGTCTATCCATTATGCAACAATGTCAAGGAAACTTATTAATTGCTTCTACATTGACAAAAAATACGCTGGTTATTTTgg                                                                                                                                                                                                                         >  1:537341/1‑219 (MQ=255)                                                           ggATCAGAGAGTAGAATTATGGGTTTCTGATAATGGTTCCGGTTGGCCGTTGTCAGATAAATTACTACAACCTTTCTCCAGTAGTAAATCCATTAATTTAGGCTTAGGATTATCAATAAGTCTATCCATTATGCAACAATGTCAAGGAAACTTATTAATTGCTTCTACATTGACAAAAAATACGCTGGTTATTTTGGAATTTAAGGTGGTAGAt                                                                                                                                                                                                        >  1:738622/1‑214 (MQ=255)                                                            gATCAGAGAGTAGAATTATGGGTTTCTGATAATGGTTCCGGTTGGCCGTTGTCAGATAAATTACTACAACCTTTCTCCAGTAGTAAATCCATTAATTTAGGCTTAGGATTATCAATAAGTCTATCCATTATGCAACAATGTCAAGGAAACTTATTAATTGCTTCTACATTGACAAAAAATACGCTGGTTATTTTGGAATTTAAGGTGGTAGATAATGTTTAATTATCAGAATAATGTTTTATTAATtgatg                                                                                                                                                                  >  1:233254/1‑251 (MQ=255)                                                                        gAATTATGGGTTTCTGATAATGGTTCCGGTTGGCCGTTGTCAGATAAATTACTACAACCTTTCTCCAGTAGTAAATCCATTAATTTAGGCTTAGGATTATCAATAAGTCTATCCATTATGCAACAATGTCAAGGAAACTTATTAATTGCTTCTACATTGACAAAAAATACGCTGGTTATTTTGGAATTTAAGGTGGTAGATAATGTTTAATTATCAGAATAATGTTTTATTAATTGATGATGATTTTGata                                                                                                                                                      >  1:673044/1‑251 (MQ=255)                                                                         aaTTATGGGTTTCTGATAATGGTTCCGGTTGGCCGTTGTCAGATAAATTACTACAACCTTTCTCCAGTAGTAAATCCATTAATTTAGGCTTAGGATTATCAATAAGTCTATCCATTATGCAACAATGTCAAGGAAACTTATTAATTGCTTCTACATTGACAAAAAATACGCTGGTTATTTTGGAATTTAAGGTGGTAGATaa                                                                                                                                                                                                      <  1:692097/202‑1 (MQ=255)                                                                          aTTATGGGTTTCTGATAATGGTTCCGGTTGGCCGTTGTCAGATAAATTACTACAACCTTTCTCCAGTAGTAAATCCATTAATTTAGGCTTAGGATTATCAATAAGTCTATCCATTATGCAACAATGTCAAGGAAACTTATTAATTGCTTCTACATTGACAAAAAATACGCTGGTTATTTTGGAATTTAAGGTGGTAGATAATGTTTAATTATCAGAATAATGt                                                                                                                                                                                >  1:682195/1‑223 (MQ=255)                                                                          aTTATGGGTTTCTGATAATGGTTCCGGTTGGCCGTTGTCAGATAAATTACTACAACCTTTCTCCAGTAGTAAATCCATTAATTTAGGCTTAGGATTATCAATAAGTCTATCCATTATGCAACAATGTCAAGGAAACTTATTAATTGCTTCTACATTGACAAAAAATACGCTGGTTATTTTGGAATTTAAGGTGGTAGATAATGTTTAATTATCAGAATAATGTTTTATTAATTGATGATGATTTTGATAtt                                                                                                                                                    >  1:328251/1‑251 (MQ=255)                                                                                       gATAATGGTTCCGGTTGGCCGTTGTCAGATAAATTACTACAACCTTTCTCCAGTAGTAAATCCATTAATTTAGGCTTAGGATTATCAATAAGTCTATCCATTATGCAACAATGTCAAGGAAACTTATTAATTGCTTCTACATTGACAAAAAATACGCTGGTTATTTTGGAATTTAAGGTGGTAGATAAt                                                                                                                                                                                                     <  1:488371/189‑1 (MQ=255)                                                                                        aTAATGGTTCCGGTTGGCCGTTGTCAGATAAATTACTACAACCTTTCTCCAGTAGTAAATCCATTAATTTAGGCTTAGGATTATCAATAAGTCTATCCATTATGCAACAATGTCAAGGAAACTTATTAATTGCTTCTACATTGACAAAAAATACGCTGGTTATTTTGGAATTTAAGGTGGTAGATAATGTTTAATTATCAGAATAATGTTTTATTAATTGATGATGATTTTGATATTTTGGAATCGTATGc                                                                                                                                      <  1:483987/251‑1 (MQ=255)                                                                                                                                aCCTTTCTCCAGTAGTAAATCCATTAATTTAGGCTTAGGATTATCAATAAGTCTATCCATTATGCAACAATGTCAAGGAAACTTATTAATTGCTTCTACATTGACAAAAAATACGCTGGTTATTTTGGAATTTAAGGTGGTAGATAATGTTTAATTATCAGAATAATGTTTTATTAATTGATGATGATTTTGATATTTTGGAATCGTATGCCGATTTATTACAACAAGAAGGCTATTCTGTATTTGCaa                                                                                                >  1:373759/1‑249 (MQ=255)                                                                                                                                aCCTTTCTCCAGTAGTAAATCCATTAATTTAGGCTTAGGATTATCAATAAGTCTATCCATTATGCAACAATGTCAAGGAAACTTATTAATTGCTTCTACATTGACAAAAAATACGCTGGTTATTTTGGAATTTAAGGTGGTAGATAATGTTTAATTATCAGAATAATGTTTTATTAATTGATGATGATTTTGATATTTTGGAATCGTATGCCGATTTATTACAACAAGAAGGCTATTCTGTATTTGCaa                                                                                                >  1:373799/1‑249 (MQ=255)                                                                                                                                aCCTTTCTCCAGTAGTAAATCCATTAATTTAGGCTTAGGATTATCAATAAGTCTATCCATTATGCAACAATGTCAAGGAAACTTATTAATTGCTTCTACATTGACAAAAAATACGCTGGTTATTTTGGAATTTAAGGTGGTAGATAATGTTTAATTATCAGAATAATGTTTTATTAATTGATGATGATTTTGATATTTTGGAATCGTATGCCGATTTATTACAACAAGAAGGCTATTCTGTATTTGCaa                                                                                                >  1:510750/1‑249 (MQ=255)                                                                                                                                                         aaTTTAGGCTTAGGATTATCAATAAGTCTATCCATTATGCAACAATGTCAAGGAAACTTATTAATTGCTTCTACATTGACAAAAAATACGCTGGTTATTTTGGAATTTAAGGTGGTAGATAATGTTTAATTATCAGAATAATGTTTTATTAATTGATGATGATTTTGATATTTTGGaa                                                                                                                                              >  1:56712/1‑178 (MQ=255)                                                                                                                                                               ggCTTAGGATTATCAATAAGTCTATCCATTATGCAACAATGTCAAGGAAACTTATTAATTGCTTCTACATTGACAAAAAATACGCTGGTTATTTTGGAATTTAAGGTGGTAGATAATGTTTAATTATCAGAATAATGTTTTATTAATTGATGATGATTTTGATATTTTGGAATCGTATGCCGATTTATTACAACAAGAAGGCTATTCTGTATTTGCAAc                                                                                               >  1:417966/1‑219 (MQ=255)                                                                                                                                                               ggCTTAGGATTATCAATAAGTCTATCCATTATGCAACAATGTCAAGGAAACTTATTAATTGCTTCTACATTGACAAAAAATACGCTGGTTATTTTGGAATTTAAGGTGGTAGATAATGTTTAATTATCAGAATAATGTTTTATTAATTGATGATGATTTTGATATTTTGGAATCGTATGCCGATTTATTACAACAAGAAGGCTATTCTGTATTTGCAACTGCCGAACCTAAAgaga                                                                              >  1:114138/1‑236 (MQ=255)                                                                                                                                                               ggCTTAGGATTATCAATAAGTCTATCCATTATGCAACAATGTCAAGGAAACTTATTAATTGCTTCTACATTGACAAAAAATACGCTGGTTATTTTGGAATTTAAGGTGGTAGATAATGTTTAATTATCAGAATAATGTTTTATTAATTGATGATGATTTTGATATTTTGGAATCGTATGCCGATTTATTACAACAAGAAGGCTATTCTGTATTTGCAACTGCCGAACCTAAAGAGATTATTAATCAAAtt                                                                >  1:139963/1‑250 (MQ=255)                                                                                                                                                                          aTCAATAAGTCTATCCATTATGCAACAATGTCAAGGAAACTTATTAATTGCTTCTACATTGACAAAAAATACGCTGGTTAtttt                                                                                                                                                                                                                           <  1:201270/84‑1 (MQ=255)                                                                                                                                                                          aTCAATAAGTCTATCCATTATGCAACAATGTCAAGGAAACTTATTAATTGCTTCTACATTGACAAAAAATACGCTGGTTATTTTGGAATTTAAGGTGGTAGATAATGTTTAATTATCAGAATAATGTTTTATTAATTGATGATGATTTTGATATTTTGGAATCGTATGCCGATTTATTACAACAAGAAGGCTATTCTGTATTTGCAACTGCCGAACCTAAAGAGATTATTAATCAAATTCCAGAAAATTgg                                                    >  1:103449/1‑251 (MQ=255)                                                                                                                                                                                aaGTCTATCCATTATGCAACAATGTCAAGGAAACTTATTAATTGCTTCTACATTGACAAAAAATACGCTGGTTATTTTGGAATTTAAGGTGGTAGAt                                                                                                                                                                                                        >  1:256939/1‑97 (MQ=255)                                                                                                                                                                                 aGTCTATCCATTATGCAACAATGTCAAGGAAACTTATTAATTGCTTCTACATTGACAAAAAATACGCTGGTTATTTTGGAATTTAAGGTGGTAGATAATGTTTAATTATCAGAATAATGTTTTATTAATTgatgatgat                                                                                                                                                             <  1:48236/139‑1 (MQ=255)                                                                                                                                                                                 aGTCTATCCATTATGCAACAATGTCAAGGAAACTTATTAATTGCTTCTACATTGACAAAAAATACGCTGGTTATTTTGGAATTTAAGGTGGTAGATAATGTTTAATTATCAGAATAATGTTTTATTAATTGATGATGATTTTGATATTTTGGAATCGTATGCCGATTTATTACAACAAGAAGGCTATTCTGTATTTGCAAc                                                                                               >  1:667499/1‑201 (MQ=255)                                                                                                                                                                                 aGTCTATCCATTATGCAACAATGTCAAGGAAACTTATTAATTGCTTCTACATTGACAAAAAATACGCTGGTTATTTTGGAATTTAAGGTGGTAGATAATGTTTAATTATCAGAATAATGTTTTATTAATTGATGATGATTTTGATATTTTGGAATCGTATGCCGATTTATTACAACAAGAAGGCTATTCTGTATTTGCAACTGCCGAACCTAAAGAGATTATTAATCAAATTCCAGAAAATTGGTATGGCg                                             >  1:837162/1‑251 (MQ=255)                                                                                                                                                                                             aTGCAACAATGTCAAGGAAACTTATTAATTGCTTCTACATTGACAAAAAATACGCTGGTTATTTTGGAATTTAAGGTGGTAGAt                                                                                                                                                                                                        >  1:600520/1‑84 (MQ=255)                                                                                                                                                                                             aTGCAACAATGTCAAGGAAACTTATTAATTGCTTCTACATTGACAAAAAATACGCTGGTTATTTTGGAATTTAAGGTGGTAGATAATGTTTAATTATCAGAATAATGt                                                                                                                                                                                <  1:342493/108‑1 (MQ=255)                                                                                                                                                                                                     aTGTCAAGGAAACTTATTAATTGCTTCTACATTGACAAAAAATACGCTGGTTATTTTGGAATTTAAGGTGGTAGATAATGTTTAATTATCAGAATAATGTTTTATTAATTGATGATGATTTTGATATTTTGGAATCGTATGCCGATTTATTACAACAAGAAGGCTATTCTGTATTTGCAACTGCCGAACCTAAAGAGATTATTAATCAAATTCCAGAAAATTGGTATGGCGTTGTGATTTGCGACGtatta                         >  1:40952/1‑251 (MQ=255)                                                                                                                                                                                                     aTGTCAAGGAAACTTATTAATTGCTTCTACATTGACAAAAAATACGCTGGTTATTTTGGAATTTAAGGTGGTAGATAATGTTTAATTATCAGAATAATGTTTTATTAATTGATGATGATTTTGATATTTTGGAATCGTATGCCGATTTATTACAACAAGAAGGCTATTCTGTATTTGCAACTGCCGAACCTAAAGAGATTATTAATCAAATTCCAGAAAATTGGTATGGCGTTGTGATTTGCGACGtatta                         >  1:261453/1‑251 (MQ=255)                                                                                                                                                                                                        tCAAGGAAACTTATTAATTGCTTCTACATTGACAAAAAATACGCTGGTTATTTTGGAATTTAAGGTGGTAGATAATGTTTAATTATCAGAATAATGTTTTATTAATTGATGATGATTTTGATATTTTGGAATCGTATGCCGATTTATTACAACAAGAAGGCTATTCTGTATTTGCAACTGCCGAACCTAAAGAGATTATTAATCAAATTCCAGAAAATTGGTATGGCGTTGTGATTTGCGACGTATTATTg                      <  1:578002/251‑1 (MQ=255)                                                                                                                                                                                                                aCTTATTAATTGCTTCTACATTGACAAAAAATACGCTGGTTATTTTGGAATTTAAGGTGGTAGATAATGTTTAATTATCAGAATAATGTTTTATTAATTGATGATGATTTTGATATTTTGGAATCGTATGCCGATTTATTACAACAAGAAGGCTATTCTGTATTTGCAACTGCCGAACCTAAAGAGATTATTAATCAAATTCCAGAAAAt                                                       >  1:178215/1‑210 (MQ=255)                                                                                                                                                                                                                 cTTATTAATTGCTTCTACATTGACAAAAAATACGCTGGTTATTTTGGAATTTAAGGTGGTAGATAATGTTTAAt                                                                                                                                                                                              <  1:651283/74‑1 (MQ=255)                                                                                                                                                                                                                 cTTATTAATTGCTTCTACATTGACAAAAAATACGCTGGTTATTTTGGAATTTAAGGTGGTAGATAATGTTTAATTATCAGAATAATGTTTTATTAATTGATGATGATTTTGATATTTTGGAATCGTATGCCGATTTATTacaaca                                                                                                                       >  1:306307/1‑145 (MQ=255)                                                                                                                                                                                                                   tattaATTGCTTCTACATTGACAAAAAATACGCTGGTTATTTTGGAATTTAAGGTGGTAGATAATGTTTAATTATCAGAATAATGTTTTATTAATTGATGATGATTTTGATATTTTGGAATCGTATGCCGATTTATTACAACAAGAAGGCTATTCTGTATTTGCAACTGCCGAACCTAAAGAGATTATTAATCAAATTCCAGAAAATTGGTATGGCGTTGTGa                                       >  1:763912/1‑223 (MQ=255)                                                                                                                                                                                                                            cTTCTACATTGACAAAAAATACGCTGGTTATTTTGGAATTTAAGGTGGTAGATAATGTTTAATTATCAGAATAATGTTTTATTAATTGATGATGATTTTGATATTTTGGAATCGTATGCCGATTTATTACAACaagaa                                                                                                                   >  1:283099/1‑138 (MQ=255)                                                                                                                                                                                                                            cTTCTACATTGACAAAAAATACGCTGGTTATTTTGGAATTTAAGGTGGTAGATAATGTTTAATTATCAGAATAATGTTTTATTAATTGATGATGATTTTGATATTTTGGAATCGTATGCCGATTTATTACAACAAGAAGGCTATTCTGTATTTGCAACTGCCGAACCTAAAGAGATTATTAATCAAATTCCAGAAAATTGGTATGGCGTTGTGATTTGCGACGTATTATTGCTGGGCATTTCAGgattaga  >  1:196323/1‑251 (MQ=255)                                                                                                                                                                                                                             ttCTACATTGACAAAAAATACGCTGGTTATTTTGGAATTTAAGGTGGTAGATAATGTTTAATTATCAGAATAATGTTTTATTAATTGATGATGATTTTGATATTTTGGAATCGTATGCCGATTTATTACAACAAGAAGGCTATTCTGTATTTGCAACTGCCGAACCTAAAGAGATTATTAATCAAATTCCAGAAAATTGGTATGGCGTTGTGATTTGCGACGtattat                        >  1:737832/1‑228 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                       CACTGGAAGCTATGCAGCATGAAAAACCGGAGATTGAGATAAAAATACAAAATATATCGGATCAGAGAGTAGAATTATGGGTTTCTGATAATGGTTCCGGTTGGCCGTTGTCAGATAAATTACTACAACCTTTCTCCAGTAGTAAATCCATTAATTTAGGCTTAGGATTATCAATAAGTCTATCCATTATGCAACAATGTCAAGGAAACTTATTAATTGCTTCTACATTGACAAAAAATGCGCTGGTTATTTTGGAATTTAAGGTGGTAGATAATGTTTAATTATCAGAATAATGTTTTATTAATTGATGATGATTTTGATATTTTGGAATCGTATGCCGATTTATTACAACAAGAAGGCTATTCTGTATTTGCAACTGCCGAACCTAAAGAGATTATTAATCAAATTCCGGAAAATTGGTATGGCGTTGTGATTTGCGACGTATTATTGCTGGGCATTTCAGGATTAGA  >  1/1151255‑1151724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑