Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,143,459	T→C	intergenic (+6410/‑16045)	unknown → / → unknown	LSU rRNA ## 23S rRNA, large subunit ribosomal RNA/tRNA‑Val‑GAC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,143,459	0	T	C	100.0%	199.8 / NA	74	intergenic (+6410/‑16045)	unknown/unknown	LSU rRNA ## 23S rRNA, large subunit ribosomal RNA/tRNA‑Val‑GAC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (41/33);  total (41/33)

GTTATACGCAGGTCGTTTGGCAGAAGATTTGATTTACGGTGAAGAAAATATTTCTACCGGGGCGTCCAATGATATTAAAGTGGCAACCAACATCGCCCGTAATATGGTGACTCAGTGGGGTTTCTCCGATAAACTTGGTCCAATTCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGTGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTACACGCGATGAAAGATGCGCTGGTGAAATACGAAACTATTGAAGAAGAACAAATCAAGCAGTTAATGAATCGTCAACCAGTGACACCGCCTCCAGGTTGGGAAGAACCAAAGAATACAGATAAAGCCGAACCTCAGCAGCCGAAAGCTGAAGAGCCAAAAATGGCAGATGATGCGCAGGGC  >  1/1143213‑1143702                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    gTTATACGCAGGTCGTTTGGCAGAAGATTTGATTTACGGTGAAGAAAATATTTCTACCGGGGCGTCCAATGATATTAAAGTGGCAACCAACATCGCCCGTAATATGGTGACTCAGTGGGGTTTCTCCGATAAACTTGGTCCAATTCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTa                                                                                                                                                                                                                                                 >  1:468228/1‑251 (MQ=255) gTTATACGCAGGTCGTTTGGCAGAAGATTTGATTTACGGTGAAGAAAATATTTCTACCGGGGCGTCCAATGATATTAAAGTGGCAACCAACATCGCCCGTAATATGGTGACTCAGTGGGGTTTCTCCGATAAACTTGGTCCAATTCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTa                                                                                                                                                                                                                                                 >  1:782771/1‑251 (MQ=255)    ataCGCAGGTCGTTTGGCAGAAGATTTGATTTACGGTGAAGAAAATATTTCTACCGGGGCGTCCAATGATATTAAAGTGGCAACCAACATCGCCCGTAATATGGTGACTCAGTGGGGTTTCTCCGATAAACTTGGTCCAATTCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATtg                                                                                                                                                                                                                                              <  1:302301/251‑1 (MQ=255)    ataCGCAGGTCGTTTGGCAGAAGATTTGATTTACGGTGAAGAAAATATTTCTACCGGGGCGTCCAATGATATTAAAGTGGCAACCAACATCGCCCGTAATATGGTGACTCAGTGGGGTTTCTCCGATAAACTTGGTCCAATTCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATtg                                                                                                                                                                                                                                              <  1:196864/251‑1 (MQ=255)            gTCGTTTGGCAGAAGATTTGATTTACGGTGAAGAAAATATTTCTACCGGGGCGTCCAATGATATTAAAGTGGCAACCAACATCGCCCGTAATATGGTGACTCAGTGGGGTTTCTCCGATAAACTTGGTCCAATTCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAAc                                                                                                                                                                                                                                         <  1:248741/248‑1 (MQ=255)             tCGTTTGGCAGAAGATTTGATTTACGGTGAAGAAAATATTTCTACCGGGGCGTCCAATGATATTAAAGTGGCAACCAACATCGCCCGTAATATGGTGACTCAGTGGGGTTTCTCCGATAAACTTGGTCCAATTCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATtg                                                                                                                                                                                                                                              >  1:84986/1‑242 (MQ=255)             tCGTTTGGCAGAAGATTTGATTTACGGTGAAGAAAATATTTCTACCGGGGCGTCCAATGATATTAAAGTGGCAACCAACATCGCCCGTAATATGGTGACTCAGTGGGGTTTCTCCGATAAACTTGGTCCAATTCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATtg                                                                                                                                                                                                                                              >  1:788609/1‑242 (MQ=255)                                          aagaaAATATTTCTACCGGGGCGTCCAATGATATTAAAGTGGCAACCAACATCGCCCGTAATATGGTGACTCAGTGGGGTTTCTCCGATAAACTTGGTCCAATTCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAAt                                                                                                                                                                                                         <  1:473347/250‑1 (MQ=255)                                                 tatTTCTACCGGGGCGTCCAATGATATTAAAGTGGCAACCAACATCGCCCGTAATATGGTGACTCAGTGGGGTTTCTCCGATAAACTTGGTCCAATTCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGataata                                                                                                                                                                                                 >  1:806100/1‑251 (MQ=255)                                                      cTACCGGGGCGTCCAATGATATTAAAGTGGCAACCAACATCGCCCGTAATATGGTGACTCAGTGGGGTTTCTCCGATAAACTTGGTCCAATTCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATgg                                                                                                                                                                                              >  1:408653/1‑249 (MQ=255)                                                      cTACCGGGGCGTCCAATGATATTAAAGTGGCAACCAACATCGCCCGTAATATGGTGACTCAGTGGGGTTTCTCCGATAAACTTGGTCCAATTCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGat                                                                                                                                                                                            >  1:185044/1‑251 (MQ=255)                                                      cTACCGGGGCGTCCAATGATATTAAAGTGGCAACCAACATCGCCCGTAATATGGTGACTCAGTGGGGTTTCTCCGATAAACTTGGTCCAATTCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGat                                                                                                                                                                                            <  1:742960/251‑1 (MQ=255)                                                        aCCGGGGCGTCCAATGATATTAAAGTGGCAACCAACATCGCCCGTAATATGGTGACTCAGTGGGGTTTCTCCGATAAACTTGGTCCAATTCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGatat                                                                                                                                                                                          <  1:548652/251‑1 (MQ=255)                                                          cGGGGCGTCCAATGATATTAAAGTGGCAACCAACATCGCCCGTAATATGGTGACTCAGTGGGGTTTCTCCGATAAACTTGGTCCAATTCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTc                                                                                                                                                                                        <  1:474771/251‑1 (MQ=255)                                                              gCGTCCAATGATATGAAAGTGGCAACCAACATCGCCCGTAATATGGTGACTCAGTGGGGTTTCTCCGATAAACTTGGTCCAATTCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTac                                                                                                                                                                                     <  1:747376/250‑1 (MQ=255)                                                               cGTCCAATGATATTAAAGTGGCAACCAACATCGCCCGTAATATGGTGACTCAGTGGGGTTTCTCCGATAAACTTGGTCCAATTCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTacac                                                                                                                                                                                   <  1:661188/251‑1 (MQ=255)                                                                gTCCAATGATATTAAAGTGGCAACCAACATCGCCCGTAATATGGTGACTCAGTGGGGTTTCTCCGATAAACTTGGTCCAATTCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGa                                                                                                                                                                                                                                           <  1:637579/194‑1 (MQ=255)                                                                gTCCAATGATATTAAAGTGGCAACCAACATCGCCCGTAATATGGTGACTCAGTGGGGTTTCTCCGATAAACTTGGTCCAATTCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAAc                                                                                                                                                                                                                                         <  1:771124/196‑1 (MQ=255)                                                                gTCCAATGATATTAAAGTGGCAACCAACATCGCCCGTAATATGGTGACTCAGTGGGGTTTCTCCGATAAACTTGGTCCAATTCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTACAcg                                                                                                                                                                                  >  1:324593/1‑251 (MQ=255)                                                                        atatTAAAGTGGCAACCAACATCGCCCGTAATATGGTGACTCAGTGGGGTTTCTCCGATAAACTTGGTCCAATTCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTacac                                                                                                                                                                                   <  1:173115/242‑1 (MQ=255)                                                                        atatTAAAGTGGCAACCAACATCGCCCGTAATATGGTGACTCAGTGGGGTTTCTCCGATAAACTTGGTCCAATTCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTACACGCGATGaaa                                                                                                                                                                          >  1:680831/1‑251 (MQ=255)                                                                                  ggCAACCAACATCGCCCGTAATATGGTGACTCAGTGGGGTTTCTCCGATAAACTTGGTCCAATTCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAatat                                                                                                                                                                                                >  1:191527/1‑219 (MQ=255)                                                                                     aaccaacATCGCCCGTAATATGGTGACTCAGTGGGGTTTCTCCGATAAACTTGGTCCAATTCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTACACGCGATGAAAGa                                                                                                                                                                        >  1:879790/1‑240 (MQ=255)                                                                                     aaccaacATCGCCCGTAATATGGTGACTCAGTGGGGTTTCTCCGATAAACTTGGTCCAATTCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTACACGCGATGAAAGATGCGCTGGTGa                                                                                                                                                             >  1:728343/1‑251 (MQ=255)                                                                                     aaccaacATCGCCCGTAATATGGTGACTCAGTGGGGTTTCTCCGATAAACTTGGTCCAATTCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTACACGCGATGAAAGATGCGCTGGTGa                                                                                                                                                             >  1:751203/1‑251 (MQ=255)                                                                                       ccaacATCGCCCGTAATATGGTGACTCAGTGGGGTTTCTCCGATAAACTTGGTCCAATTCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTACACGCGATGAAAGATGCGCTGGTGaaa                                                                                                                                                           <  1:698961/251‑1 (MQ=255)                                                                                                cccGTAATATGGTGACTCAGTGGGGTTTCTCCGATAAACTTGGTCCAATTCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTACACGCGATGAAAGATGCGCTGGTGAAATACGAAAc                                                                                                                                                   <  1:17452/250‑1 (MQ=255)                                                                                                   gTAATATGGTGACTCAGTGGGGTTTCTCCGATAAACTTGGTCCAATTCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGa                                                                                                                                                                                                                                           >  1:426707/1‑159 (MQ=255)                                                                                                   gTAATATGGTGACTCAGTGGGGTTTCTCCGATAAACTTGGTCCAATTCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGat                                                                                                                                                                                                     >  1:563810/1‑197 (MQ=255)                                                                                                   gTAATATGGTGACTCAGTGGGGTTTCTCCGATAAACTTGGTCCAATTCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCt                                                                                                                                                                                       >  1:572269/1‑211 (MQ=255)                                                                                                   gTAATATGGTGACTCAGTGGGGTTTCTCCGATAAACTTGGTCCAATTCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTACACGCGATGAAAGa                                                                                                                                                                        >  1:603617/1‑226 (MQ=255)                                                                                                   gTAATATGGTGACTCAGTGGGGTTTCTCCGATAAACTTGGTCCAATTCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTACACGCGATGAAAGATGCGCTGGTGAAATACGAAACTAtt                                                                                                                                               >  1:344319/1‑251 (MQ=255)                                                                                                   gTAATATGGTGACTCAGTGGGGTTTCTCCGATAAACTTGGTCCAATTCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTACACGCGATGAAAGATGCGCTGGTGAAATACGAAACTAtt                                                                                                                                               >  1:597154/1‑251 (MQ=255)                                                                                                    tAATATGGTGACTCAGTGGGGTTTCTCCGATAAACTTGGTCCAATTCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTACACGCGATGAAAGATGCGCTGGTGAAATACGAAACTATTg                                                                                                                                              >  1:827743/1‑251 (MQ=255)                                                                                                    tAATATGGTGACTCAGTGGGGTTTCTCCGATAAACTTGGTCCAATTCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATAAGGATATTCTACACGCGATGAAAGATGCGCTGGTGAAATACGAAACTATTg                                                                                                                                              >  1:204743/1‑251 (MQ=255)                                                                                                    tAATATGGTGACTCAGTGGGGTTTCTCCGATAAACTTGGTCCAATTCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGCGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTACACGCGATGAAAGa                                                                                                                                                                        >  1:807719/1‑225 (MQ=255)                                                                                                       tatGGTGACTCAGTGGGGTTTCTCCGATAAACTTGTTCCAATTCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTTCGATGAATCCGCACATGTGATCGTTGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTACACGCGATGAAAGATGCGCTGGTGAAATACGAAACTATTgaag                                                                                                                                           <  1:860427/251‑1 (MQ=255)                                                                                                         tGGTGACTCAGTGGGGTTTCTCCGATAAACTTGGTCCAATTCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTACACGCGATGAAAGATGCGCTGGTGAAATACGAAACTATTgaagaa                                                                                                                                         >  1:253779/1‑251 (MQ=255)                                                                                                                 cAGTGGGGTTTCTCCGATAAACTTGGTCCAATTCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATgg                                                                                                                                                                                              <  1:494167/190‑1 (MQ=255)                                                                                                                 cAGTGGGGTTTCTCCGATAAACTTGGTCCAATTCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTACAcg                                                                                                                                                                                  <  1:242353/202‑1 (MQ=255)                                                                                                                 cAGTGGGGTTTCTCCGATAAACTTGGTCCAATTCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTACACGCGATGAAAGATGCGCTGGTGAAATACGAAAc                                                                                                                                                   >  1:879471/1‑233 (MQ=255)                                                                                                                    tGGGGTTTCTCCGATAAACTTGGTCCAATTCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTACACGCGATGAAAGATGCGCTGGTGAAATACGAAACTATTGAAGAAGAACAAATc                                                                                                                                <  1:500481/249‑1 (MQ=255)                                                                                                                                 aTAAACTTGGTCCAATTCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAGAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTACACGCGATGAAAGATGCGCTGGTGAAATACGAAACTATTGAAGAAGAACAAATCAAGCAGTTAATGaa                                                                                                                  <  1:73656/250‑1 (MQ=255)                                                                                                                                 aTAAACTTGGTCCAATTCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTACACGCGATGAAAGATGCGCTGGTGAAATACGAAACTATTGAAGAAGAACAAATCAAGCAGTTAATGaa                                                                                                                  <  1:115851/250‑1 (MQ=255)                                                                                                                                 aTAAACTTGGTCCAATTCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTACACGCGATGAAAGATGCGCTGGTGAAATACGAAACTATTGAAGAAGAACAAATCAAGCAGTTAATGAAt                                                                                                                 <  1:50185/251‑1 (MQ=255)                                                                                                                                     aCTTGGTCCAATTCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTACACGCGATGAAAGATGCGCTGGTGAAATACGAAACTATTGAAGAAGAACAAATCAAGCAGTTAATGAAt                                                                                                                 >  1:76397/1‑247 (MQ=255)                                                                                                                                      cTTGGTCCAATTCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGGTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGAGAGACAGATTTTAATCGATAATATGGATATTCTACACGCGATGAAAGATGCGCTGGTGAAATACGAAACTATTGAAGAAGAACAAATCAAGCAGTTAATGAATCGTCa                                                                                                            <  1:253350/251‑1 (MQ=255)                                                                                                                                                ttCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTACACGCGATGAAAGATGCGCTGGTGAAATACGAAACTATTGAAGAAGAACAAATCAAGCAGTTAATGAATCGTCAACCAGTGaca                                                                                                  >  1:333414/1‑251 (MQ=255)                                                                                                                                                 tCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAAc                                                                                                                                                                                                                                         <  1:675334/115‑1 (MQ=255)                                                                                                                                                 tCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAAc                                                                                                                                                                                                                                         >  1:480159/1‑115 (MQ=255)                                                                                                                                                                          gTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTACACGCGATGAAAGATGCGCTGGTGAAATACGAAACTATTGAAGAAGAACAAATc                                                                                                                                <  1:268187/195‑1 (MQ=255)                                                                                                                                                                          gTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTACACGCGATGAAAGATGCGCTGGTGAAATACGAAACTATTGAAGAAGAACAAATCAAGCAGTTAATGAATCGTCAACCAGTGacac                                                                                                 >  1:630750/1‑226 (MQ=255)                                                                                                                                                                          gTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTACACGCGATGAAAGATGCGCTGGTGAAATACGAAACTATTGAAGAAGAACAAATCAAGCAGTTAATGAATCGTCAACCAGTGACACCGCCTCCAGGTTGGGAAGAACCaaa                                                                        >  1:756235/1‑251 (MQ=255)                                                                                                                                                                             ttCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTACACGCGATGAAAGAt                                                                                                                                                                       >  1:221323/1‑153 (MQ=255)                                                                                                                                                                             ttCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTACACGCGATGAAAGATGCGCTGGTGAAATACGAAACTATTgaag                                                                                                                                           <  1:399534/181‑1 (MQ=255)                                                                                                                                                                             ttCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTACACGCGATGAAAGATGCGCTGGTGAAATACGAAACTATTGAAGAAGAACAAATCAAGCAGTTAATGAATCGTCAACCAGTGac                                                                                                   >  1:837053/1‑221 (MQ=255)                                                                                                                                                                             ttCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTACACGCGATGAAAGATGCGCTGGTGAAATACGAAACTATTGAAGAAGAACAAATCAAGCAGTTAATGAATCGTCAACCAGTGACACCGCCTCCAGGTTGGGAAGAACCAAAGaa                                                                     >  1:708839/1‑251 (MQ=255)                                                                                                                                                                             ttCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTACACGCGATGAAAGATGCGCTGGTGAAATACGAAACTATTGAAGAAGAACAAATCAAGCAGTTAATGAATCGTCAACCAGTGACACCGCCTCCAGGTTGGGAAGAACCAAAGaa                                                                     >  1:357850/1‑251 (MQ=255)                                                                                                                                                                             ttCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTACACGCGATGAAAGATGCGCTGGTGAAATACGAAACTATTGAAGAAGAACAAATCAAGCAGTTAATGAATCGTCAACCAGTGACACCGCCTCCAGGTTGGGAAGAACCAAAGaa                                                                     >  1:43127/1‑251 (MQ=255)                                                                                                                                                                                   gggCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTACACGCGATGAAAGATGCGCTGGTGAAATACGAAACTATTGAAGAAGAACAAATCAAGCAGTTAATGAATCGTCAACCAGTGACACCGCCTCCAGGTTGGGAAGAAcc                                                                           <  1:836128/239‑1 (MQ=255)                                                                                                                                                                                        ttCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTACACGCGATGAAAGATGCGCTGGTGAAATACGAAACTATTGAAGAAGAACAAATCAAGCAGTTAAt                                                                                                                     <  1:458206/192‑1 (MQ=255)                                                                                                                                                                                                     gCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTACACGCGATGAAAGATGCGCTGGTGAAATACGAAACTATTGAAGAAGAACAAATCAAGCAGTTAATGAATCGTCAACCAGTGACACCGCCTCCAGGTTGGGAAGAACCAAAGAATACAGATAAAGc                                                         >  1:464157/1‑239 (MQ=255)                                                                                                                                                                                                      ccAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTACACGCGATGAAAGATGCGCTGGTGAAATACGAAACTATTGAAGAAGAACAAATCAAGCAGTTAATGAATCGTCAACCAGTGACACCGCCTCCAGGTTGGGAAGAACCAAAGAATACAGATAAAGCTGAACCTcagcag                                            >  1:202221/1‑251 (MQ=255)                                                                                                                                                                                                           cacaTGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTACACGCGATGAAAGATGCGCTGGTGAAATACGAAACTATTGAAGAAGAACAAATCAAGCAGTTAATGAATCGTCAACCAGTGACACCGCCTCCAGGTTGGGAAGAACCAAAGAATACAGATAAAGc                                                         >  1:337451/1‑233 (MQ=255)                                                                                                                                                                                                           cacaTGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTACACGCGATGAAAGATGCGCTGGTGAAATACGAAACTATTGAAGAAGAACAAATCAAGCAGTTAATGAATCGTCAACCAGTGACACCGCCTCCAGGTTGGGAAGAACCAAAGAATACAGATAAAGCTGAACCTCAGCAGCCGaa                                       >  1:735712/1‑251 (MQ=255)                                                                                                                                                                                                           cacaTGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTACACGCGATGAAAGATGCGCTGGTGAAATACGAAACTATTGAAGAAGAACAAATCAAGCAGTTAATGAATCGTCAACCAGTGACACCGCCTCCAGGTTGGGAAGAACCAAAGAATACAGATAAAGCTGAACCTCAGCAGCCGaa                                       >  1:753154/1‑251 (MQ=255)                                                                                                                                                                                                              aTGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTACACGCGATGAAAGATGCGCTGGTGAAATACGAAACTATTGAAGAAGAACAAATCAAGCAGTTAATGAATCGTCAACCAGTGACACCGCCTCCAGGTTGGGAAGAACCAAAGAATACAGATAAAGCTGAACCTCAGCAGCCGAAAGc                                    <  1:388374/251‑1 (MQ=255)                                                                                                                                                                                                                     aTGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTACACGCGATGAAAGATGCGCTGGTGAAATACGAAACTATTGAAGAAGAACAAATCAAGCAGTTAATGAATCGTCAACCAGTGACACCGCCTCCAGGTTGGGAAGAACCAAAg                                                                       <  1:254410/209‑1 (MQ=255)                                                                                                                                                                                                                     aTGAAACCGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTACACGCGATGAAAGATGCGCTGGTGAAATACGAAACTATTGAAGAAGAACAAATCAAGCAGTTAATGAATCGTCAACCAGTGACACCGCCTCCAGGTTGGGAAGAACCAAAg                                                                       <  1:688685/209‑1 (MQ=255)                                                                                                                                                                                                                           ccGCACATGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTACACGCGATGAAAGATGCGCTGGTGAAATACGAAACTATTGAAGAAGAACAAATCAAGCAGTTAATGaa                                                                                                                  >  1:877886/1‑160 (MQ=255)                                                                                                                                                                                                                                caTGTGATCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTACACGCGATGAAAGATGCGCTGGTGAAATACGAAACTATTGAAGAAGAACAAATCAAGCAGTTAATGAATCGTCAACCAGTGACACCGCCTCCAGGTTGGGAAGAACCAAAGAATACAGATAAAGCTGAACCTCAGCAGCCGAAAGCTGAAGAGCCAAAAATGGc                  <  1:164982/251‑1 (MQ=255)                                                                                                                                                                                                                                      aTCGATGAGGAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTACACGCGATGAAAGATGCGCTGGTGAAATACGAAACTATTGAAGAAGAACAAATCAAGCAGTTAATGAATCGTCAACCAGTGACACCGCCTCCAGGTTGGGAAGAACCAAAGAATACAGATaaa                                                           >  1:274397/1‑204 (MQ=255)                                                                                                                                                                                                                                               gAAGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTACACGCGATGAAAGATGCGCTGGTGAAATACGAAACTATTGAAGAAGAACAAATCAAGCAGTTAATGAATCGTCAACCAGTGACACCGCCTCCAGGTTGGGAAGAACCAAAGAATACAGATAAAGCTGAACCTCAGCAGCCGAAAGCTGAAGAGCCAAAAATGGCAGATGATGCGCAg     <  1:839161/249‑1 (MQ=255)                                                                                                                                                                                                                                                aaGTTCGCGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTACACGCGATGAAAGATGCGCTGGTGAAATACGAAACTATTGAAGAAGAACAAATCAAGCAGTTAATGAATCGTCAACCAGTGACACCGCCTCCAGGTTGGGAAGAACCAAAGAATACAGATAAAGCTGAACCTCAGCAGCCGAAAGCTGAAGAGCCAAAAATGGCAGATGATGCGCAGGGc  <  1:691323/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                    GTTATACGCAGGTCGTTTGGCAGAAGATTTGATTTACGGTGAAGAAAATATTTCTACCGGGGCGTCCAATGATATTAAAGTGGCAACCAACATCGCCCGTAATATGGTGACTCAGTGGGGTTTCTCCGATAAACTTGGTCCAATTCTTTATGCCGAAGACGACGGGGAAGTATTCTTAGGGCGTTCCATGGCGAAAGCCAAACACATGTCCGATGAAACCGCACATGTGATCGATGAGGAAGTTCGTGCTATTGTGAACCGAAATTATGGTCGCGCGAGACAGATTTTAATCGATAATATGGATATTCTACACGCGATGAAAGATGCGCTGGTGAAATACGAAACTATTGAAGAAGAACAAATCAAGCAGTTAATGAATCGTCAACCAGTGACACCGCCTCCAGGTTGGGAAGAACCAAAGAATACAGATAAAGCCGAACCTCAGCAGCCGAAAGCTGAAGAGCCAAAAATGGCAGATGATGCGCAGGGC  >  1/1143213‑1143702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑