Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,141,795	A→C	intergenic (+4746/‑17709)	unknown → / → unknown	LSU rRNA ## 23S rRNA, large subunit ribosomal RNA/tRNA‑Val‑GAC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,141,795	0	A	C	100.0%	99.8 / NA	37	intergenic (+4746/‑17709)	unknown/unknown	LSU rRNA ## 23S rRNA, large subunit ribosomal RNA/tRNA‑Val‑GAC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (20/17);  total (20/17)

ACTTAAAAGAGATCCGTTCTCTGTTTAGTGTAGTCAAAGTTCGTAAACCTGAAGCCTCCCGAGACCGATCTCGGGAAGTTTATATTGTTGCAAGCGGTTATAAACACTAATCAAATTAAGTTACCAATAAGCTCAATTGATTTGATAACTTTAATTTTTGAGATAAATATAGTAACCTTACAACACGTTGTTAACTTATAAATAAGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCCGGCGGAGGGATAACGGATTATACGACATTCATTTCGGATGTTGAAAATAATCAGGTACGTCAGGCTAAATTTGAAGATAATGAAATTTTAGTCACTAAAACCGACGGCACTAAATATACAACAGTTATTCCATTGGAAGACAAAGAT  >  1/1141591‑1142040                                                                                                                                                                                                             |                                                                                                                                                                                                                                                      aCTTAAAAGAGATCCGTTCTCTGTTTAGTGTAGTCAAAGTTCGTAAACCTGAAGCCTCCCGAGACCGATCTCGGGAAGTTTATATTGTTGCAAGCGGTTATAAACACTAATCAAATTAAGTTACCAATAAGCTCAATTGATTTGATAACTTTAATTTTTGAGATAAATATAGTAACCTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAAc                                                                                                                                                                                                                                      >  1:481209/1‑222 (MQ=255)                       gTTTAGTGTAGTCAAAGTTCGTAAACCTGAAGCCTCCCGAGACCGATCTCGGGAAGTTTATATTGTTGCAAGCGGTTATAAACACTAATCAAATTAAGTTACCAATAAGCTCAATTGATTTGATAACTTTAATTTTTGAGATAAATATAGTAACCTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAAc                                                                                                                                                                                                                                      <  1:585356/200‑1 (MQ=255)                       gTTTAGTGTAGTCAAAGTTCGTAAACCTGAAGCCTCCCGAGACCGATCTCGGGAAGTTTATATTGTTGCAAGCGGTTATAAACACTAATCAAATTAAGTTACCAATAAGCTCAATTGATTTGATAACTTTAATTTTTGAGATAAATATAGTAACCTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCatgatg                                                                                                                                                                                   >  1:455466/1‑251 (MQ=255)                       gTTTAGTGTAGTCAAAGTTCGTAAACCTGAAGCCTCCCGAGACCGATCTCGGGAAGTTTATATTGTTGCAAGCGGTTATAAACACTAATCAAATTAAGTTACCAATAAGCTCAATTGATTTGATAACTTTAATTTTTGAGATAAATATAGTAACCTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCatga                                                                                                                                                                                     >  1:659639/1‑249 (MQ=255)                                              aaCCTGAAGCCTCCCGAGACCGATCTCGGGAAGTTTATATTGTTGCAAGCGGTTATAAACACTAATCAAATTAAGTTACCAATAAGCTCAATTGATTTGATAACTTTAATTTTTGAGATAAATATAGTAACCTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATAc                                                                                                                                                            <  1:35935/251‑1 (MQ=255)                                                        cTCCCGAGACCGATCTCGGGAAGTTTATATTGTTGCAAGCGGTTATAAACACTAATCAAATTAAGTTACCAATAAGCTCAATTGATTTGATAACTTTAATTTTTGAGATAAATATAGTAACCTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCcggc                                                                                                                                                  <  1:92344/251‑1 (MQ=255)                                                         tCCCGAGACCGATCTCGGGAAGTTTATATTGTTGCAAGCGGTTATAAACACTAATCAAATTAAGTTACCAATAAGCTCAATTGATTTGATAACTTTAATTTTTGAGATAAATATAGTAACCTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCcggcg                                                                                                                                                 >  1:249499/1‑251 (MQ=255)                                                              agaCCGATCTCGGGAAGTTTATATTGTTGCAAGCGGTTATAAACACTAATCAAATTAAGTTACCAATAAGCTCAATTGATTTGATAACTTTAATTTTTGAGATAAATATAGTAACCTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATAc                                                                                                                                                            >  1:184127/1‑235 (MQ=255)                                                                               tttATATTGTTGCAAGCGGTTATAAACACTAATCAAATTAAGTTACCAATAAGCTCAATTGATTTGATAACTTTAATTTTTGAGATAAATATAGTAACCTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCCGGCGGAGGGATAACGGATTATACGAc                                                                                                                           <  1:548284/251‑1 (MQ=255)                                                                                               cGGTTATAAACACTAATCAAATTAAGTTACCAATAAGCTCAATTGATTTGATAACTTTAATTTTTGAGATAAATATAGTAACCTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCCGGCGGAGGGATAACGGATTATACGACATTCATTTCGGATGtt                                                                                                           >  1:461357/1‑251 (MQ=255)                                                                                                 gTTATAAACACTAATCAAATTAAGTTACCAATAAGCTCAATTGATTTGATAACTTTAATTTTTGAGATAAATATAGTAACCTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTggg                                                                                                                                                                                                          <  1:110375/154‑1 (MQ=255)                                                                                                   tataAACACTAATCAAATTAAGTTACCAATAAGCTCAATTGATTTGATAACTTTAATTTTTGAGATAAATATAGTAACCTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGcc                                                                                                                                                                                               >  1:190884/1‑163 (MQ=255)                                                                                                             aaTCAAATTAAGTTACCAATAAGCTCAATTGATTTGATAACTTTAATTTTTGAGATAAATATAGTAACCTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCCGGCGGAGGGATAACGGATTATACGACATTCATTTCGGATGTTGAAAATAATCAGGt                                                                                             >  1:36761/1‑251 (MQ=255)                                                                                                                            ccAATAAGCTCAATTGATTTGATAACTTTAATTTTTGAGATAAATATAGTAACCTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCCGGCGGAGGGATAACGGATTATACGACATTCATTTCGGATGTTGAAAATAATCAGGTACGTCAGGCTAAAtt                                                                              >  1:387546/1‑251 (MQ=255)                                                                                                                                        aTTGATTTGATAACTTTAATTTTTGAGATAAATATAGTAACCTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCCGGCGGAGGGATAACGGATTATACGACATTCATTTCGGATGTTGAAAATAATCAGGTACGTCAGGCTAAATTTGAAGATAATGa                                                                  >  1:17795/1‑251 (MQ=255)                                                                                                                                           gatttgatAACTTTAATTTTTGAGATAAATATAGTAACCTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCCGGCGAAGGGATAACGGATTATACGACATTCATTTCGGATGTTGAAAATAATCAGGTACGTCa                                                                                       >  1:435483/1‑227 (MQ=255)                                                                                                                                                                  gaTAAATATAGTAACCTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCCGGCGGAGGGATAACGGATTATACGACATTCATTTCGGATGTTGAAAATAATCAGGTACGTCAGGCTAAAtt                                                                              <  1:422060/213‑1 (MQ=255)                                                                                                                                                                  gaTAAATATAGTAACCTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCCGGCGGAGGGATAACGGATTATACGACATTCATTTCGGATGTTGAAAATAATCAGGTACGTCAGGCTAAATTTGAAGATAATGAAATTTTAGTCACTAAAACCGACGGCa                                        >  1:477553/1‑251 (MQ=255)                                                                                                                                                                     aaaTATAGTAACCTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAAATTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCCGGCGGAGGGATAACGGATTATACGACATTCATTTCGGATGTTGAAAATAATCAGGTACGTCAGGCTAAATTTGAAGATAATGAAATTTTAGTCACTAAAACCGACGGCACTa                                     >  1:26601/1‑251 (MQ=255)                                                                                                                                                                       atatAGTAACCTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGcc                                                                                                                                                                                               <  1:93219/95‑1 (MQ=255)                                                                                                                                                                        tataGTAACCTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCCGGCGGAGGGATAACGGATTATACGACATTCATTTCGGATGTTGAAAATAATCAGGTACGTCAGGCTAAATTTGAAGATAATGAAATTTTAGTCACTAAAACCGACGGCACTAAat                                  >  1:582050/1‑251 (MQ=255)                                                                                                                                                                             tAACCTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCCGGCGGAGGGATAACGGATTATACGACATTCATTTCGGATGTTGAAAATAATCAGGTACGTCAGGCTAAATTTGAAGATAATGAAATTTTAGTCACTAAAACCGACGGCACTAAATATac                              <  1:41718/250‑1 (MQ=255)                                                                                                                                                                               aCCTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCCGGCGGAGGGATAACGGATTATACGACATTCATTTCGGATGTTGAAAATAATCAGGTACGTCAGGCTAAAtt                                                                              >  1:683583/1‑200 (MQ=255)                                                                                                                                                                               aCCTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCCGGCGGAGGGATAACGGATTATACGACATTCATTTCGGATGTTGAAAATAATCAGGTACGTCAGGCTAAATTTGAAGATAATGAAATTTTAGTCACTAAAACCGACGGCACTAAATATacaac                           <  1:321059/251‑1 (MQ=255)                                                                                                                                                                                ccTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCCGGCGGAGGGATAACGGATTATACGACATTCATTTCGGATg                                                                                                             >  1:23211/1‑168 (MQ=255)                                                                                                                                                                                ccTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCCGGCGGAGGGATAACGGATTATACGACATTCATTTCGGATGTTGAAAATAATCAGGTACGTCAGGCt                                                                                   >  1:858452/1‑194 (MQ=255)                                                                                                                                                                                ccTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCCGGCGGAGGGATAACGGATTATACGACATTCATTTCGGATGTTGAAAATAATCAGGTACGTCAGGCTAAATTTGAAGATAATGAAATTTTAGTc                                                        <  1:872061/221‑1 (MQ=255)                                                                                                                                                                                ccTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCCGGCGGAGGGATAACGGATTATACGACATTCATTTCGGATGTTGAAAATAATCAGGTACGTCAGGCTAAATTTGAAGATAATGAAATTTTAGTCACTAAAACCGACGGCACTAAATATacaac                           <  1:169931/250‑1 (MQ=255)                                                                                                                                                                                    acaacaTGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCCGGCGGAGGGATAACGGATTATACGACATTCATTTCGGATGTTGAAAATAATCAGGTACGTCAGGCt                                                                                   <  1:103624/190‑1 (MQ=255)                                                                                                                                                                                        catgttgttAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCCGGCGGAGGGATAACGGATTATACGACATTCATTTCGGATGTTGAAAATAGTCAGGTACGTCAGGCTAAATTTGAAGATAATGAAATTTTAGTCACTAAAACCGACGGCACTAAATATACAACAGTTATTc                   >  1:453412/4‑250 (MQ=255)                                                                                                                                                                                        catgttgttAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCCGGCGGAGGGATAACGGATTATACGACATTCATTTCGGATGTTGAAAATAATCAGGTACGTCAGGCTAAATTTGAAGATAATGAAATTTTAGTCACTAAAACCGACGGCACTAAATATACAACAGTTATTcc                  <  1:279331/248‑1 (MQ=255)                                                                                                                                                                                         atgttgttAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCCGGCGGAGGGATAACGGATTATACGACATTCATTTCGGATGTTGAAAATAATCAGGTACGTCAGGCTAAATTTGAAGATAATGAAATTTTAGTCACTAAAACCGACGGCACTAAat                                  <  1:15540/232‑1 (MQ=255)                                                                                                                                                                                             tgttAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCCGGCGGAGGGATAACGGATTATACGACATTCATTTCGGATGTTGAAAATAATCAGGTACGTCAGGCTAAATTTGAAGATAATGAAATTTTAGTCACTAAAACCGACGGCACTAAATATACAACAGTTATTCCATTgg             <  1:801514/251‑1 (MQ=255)                                                                                                                                                                                                   cTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAAt                                                                                                                                                                                                                  >  1:676196/1‑48 (MQ=255)                                                                                                                                                                                                   cTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCCGGCGGAGGGATAACGGATTATACGACATTCATTTCGGATGTTGAAAATAATCAGGTACGTCAGGCTAAATTTGAAGATAATGAAATTTTAGTCACTAAAACCGACGGCACTAAATATACAACAg                         <  1:776768/233‑1 (MQ=255)                                                                                                                                                                                                   cTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCCGGCGGAGGGATAACGGATTATACGACATTCATTTCGGATGTTGAAAATAATCAGGTACGTCAGGCTAAATTTGAAGATAATGAAATTTTAGTCACTAAAACCGACGGCACTAAATATACAACAGTTATTCCATTGGAAGACa       >  1:146378/1‑251 (MQ=255)                                                                                                                                                                                                     tataAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCCGGCGGAGGGATaa                                                                                                                                        <  1:332595/120‑1 (MQ=255)                                                                                                                                                                                                      ataaatacGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCCGGCGGAGGGATAACGGATTATACGACATTCATTTCGGATGTTGAAAATAATc                                                                                                 <  1:381579/158‑1 (MQ=255)                                                                                                                                                                                                      ataaatacGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCCGGCGGAGGGATAACGGATTATACGACATTCATTTCGGATGTTGAAAATAATCAGGTACGTCAGGCTAAATTTGAAGATAATGAAATTTTAGTCACTAAAACCGACGGCACTAAATATac                              <  1:768478/225‑1 (MQ=255)                                                                                                                                                                                                         aatacGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCCGGCGGAGGGATAACGGATTATACGACATTCATTTCGGATGTTGAAAATAATCAGGTACGTCAGGCTAAATTTGAAGATAATGAAATTTTAGTCACTAAAACCGACGGCACTAAATATACAACAGTTATTCCATTGGAAGACAAAGAt  >  1:369670/1‑250 (MQ=255)                                                                                                                                                                                                             |                                                                                                                                                                                                                                                      ACTTAAAAGAGATCCGTTCTCTGTTTAGTGTAGTCAAAGTTCGTAAACCTGAAGCCTCCCGAGACCGATCTCGGGAAGTTTATATTGTTGCAAGCGGTTATAAACACTAATCAAATTAAGTTACCAATAAGCTCAATTGATTTGATAACTTTAATTTTTGAGATAAATATAGTAACCTTACAACACGTTGTTAACTTATAAATAAGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCCGGCGGAGGGATAACGGATTATACGACATTCATTTCGGATGTTGAAAATAATCAGGTACGTCAGGCTAAATTTGAAGATAATGAAATTTTAGTCACTAAAACCGACGGCACTAAATATACAACAGTTATTCCATTGGAAGACAAAGAT  >  1/1141591‑1142040

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑