Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,141,776	C→T	intergenic (+4727/‑17728)	unknown → / → unknown	LSU rRNA ## 23S rRNA, large subunit ribosomal RNA/tRNA‑Val‑GAC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,141,776	0	C	T	100.0%	81.5 / NA	31	intergenic (+4727/‑17728)	unknown/unknown	LSU rRNA ## 23S rRNA, large subunit ribosomal RNA/tRNA‑Val‑GAC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/13);  total (18/13)

AAAGGAAGTTTTGTAGTAAAAGTTTTTCAGGGTGAAGGTTTTGATGAATACTTAAAAGAGATCCGTTCTCTGTTTAGTGTAGTCAAAGTTCGTAAACCTGAAGCCTCCCGAGACCGATCTCGGGAAGTTTATATTGTTGCAAGCGGTTATAAACACTAATCAAATTAAGTTACCAATAAGCTCAATTGATTTGATAACTTTAATTTTTGAGATAAATATAGTAACCTTACAACACGTTGTTAACTTATAAATAAGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCCGGCGGAGGGATAACGGATTATACGACATTCATTTCGGATGTTGAAAATAATCAGGTACGTCAGGCTAAATTTGAAGATAATGAAATTTTAGTCACTAAAACCGACGGCACTAAATATACAAC  >  1/1141542‑1142015                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                aaaGGAAGTTTTGTAGTAAAAGTTTTTCAGGGGGAAGGTTTTGATGAATACTTAAAAGAGATCCGTTCTCTGTTTAGTGTAGTCAAAGTTCGTAAACCTGAAGCCTCCCGAGACCGATCTCGGGAAGTTTATATTGTTGCAAGCGGTTATAAACACTAATCAAATTAAGTTACCAATAAGCTCAATTGATTTGATAACTTTAATTTTTGAGATAAATATAGTAACCTTACAACATGTTGTTAACTTataaa                                                                                                                                                                                                                                 >  1:89218/1‑251 (MQ=255)                         tttCAGGGGGAAGGTTTTGATGAATACTTAAAAGAGATCCGTTCTCTGTTTAGTGTAGTCAAAGTTCGTAAACCTGAAGCCTCCCGAGACCGATCTCGGGAAGTTTATATTGTTGCAAGCGGTTATAAACACTAATCAAATTAAGTTACCAATAAGCTCAATTGATTTGATAACTTTAATTTTTGAGATAAATATAGTAACCTTACAACATGTTGTTAACTTataaatac                                                                                                                                                                                                                              <  1:230954/230‑2 (MQ=255)                                                  aCTTAAAAGAGATCCGTTCTCTGTTTAGTGTAGTCAAAGTTCGTAAACCTGAAGCCTCCCGAGACCGATCTCGGGAAGTTTATATTGTTGCAAGCGGTTATAAACACTAATCAAATTAAGTTACCAATAAGCTCAATTGATTTGATAACTTTAATTTTTGAGATAAATATAGTAACCTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAAc                                                                                                                                                                                                             >  1:481209/1‑222 (MQ=255)                                                                        gTTTAGTGTAGTCAAAGTTCGTAAACCTGAAGCCTCCCGAGACCGATCTCGGGAAGTTTATATTGTTGCAAGCGGTTATAAACACTAATCAAATTAAGTTACCAATAAGCTCAATTGATTTGATAACTTTAATTTTTGAGATAAATATAGTAACCTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAAc                                                                                                                                                                                                             <  1:585356/200‑1 (MQ=255)                                                                        gTTTAGTGTAGTCAAAGTTCGTAAACCTGAAGCCTCCCGAGACCGATCTCGGGAAGTTTATATTGTTGCAAGCGGTTATAAACACTAATCAAATTAAGTTACCAATAAGCTCAATTGATTTGATAACTTTAATTTTTGAGATAAATATAGTAACCTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCatgatg                                                                                                                                                          >  1:455466/1‑251 (MQ=255)                                                                        gTTTAGTGTAGTCAAAGTTCGTAAACCTGAAGCCTCCCGAGACCGATCTCGGGAAGTTTATATTGTTGCAAGCGGTTATAAACACTAATCAAATTAAGTTACCAATAAGCTCAATTGATTTGATAACTTTAATTTTTGAGATAAATATAGTAACCTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCatga                                                                                                                                                            >  1:659639/1‑249 (MQ=255)                                                                                               aaCCTGAAGCCTCCCGAGACCGATCTCGGGAAGTTTATATTGTTGCAAGCGGTTATAAACACTAATCAAATTAAGTTACCAATAAGCTCAATTGATTTGATAACTTTAATTTTTGAGATAAATATAGTAACCTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATAc                                                                                                                                   <  1:35935/251‑1 (MQ=255)                                                                                                         cTCCCGAGACCGATCTCGGGAAGTTTATATTGTTGCAAGCGGTTATAAACACTAATCAAATTAAGTTACCAATAAGCTCAATTGATTTGATAACTTTAATTTTTGAGATAAATATAGTAACCTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCcggc                                                                                                                         <  1:92344/251‑1 (MQ=255)                                                                                                          tCCCGAGACCGATCTCGGGAAGTTTATATTGTTGCAAGCGGTTATAAACACTAATCAAATTAAGTTACCAATAAGCTCAATTGATTTGATAACTTTAATTTTTGAGATAAATATAGTAACCTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCcggcg                                                                                                                        >  1:249499/1‑251 (MQ=255)                                                                                                               agaCCGATCTCGGGAAGTTTATATTGTTGCAAGCGGTTATAAACACTAATCAAATTAAGTTACCAATAAGCTCAATTGATTTGATAACTTTAATTTTTGAGATAAATATAGTAACCTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATAc                                                                                                                                   >  1:184127/1‑235 (MQ=255)                                                                                                                                tttATATTGTTGCAAGCGGTTATAAACACTAATCAAATTAAGTTACCAATAAGCTCAATTGATTTGATAACTTTAATTTTTGAGATAAATATAGTAACCTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCCGGCGGAGGGATAACGGATTATACGAc                                                                                                  <  1:548284/251‑1 (MQ=255)                                                                                                                                                cGGTTATAAACACTAATCAAATTAAGTTACCAATAAGCTCAATTGATTTGATAACTTTAATTTTTGAGATAAATATAGTAACCTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCCGGCGGAGGGATAACGGATTATACGACATTCATTTCGGATGtt                                                                                  >  1:461357/1‑251 (MQ=255)                                                                                                                                                  gTTATAAACACTAATCAAATTAAGTTACCAATAAGCTCAATTGATTTGATAACTTTAATTTTTGAGATAAATATAGTAACCTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTggg                                                                                                                                                                                 <  1:110375/154‑1 (MQ=255)                                                                                                                                                    tataAACACTAATCAAATTAAGTTACCAATAAGCTCAATTGATTTGATAACTTTAATTTTTGAGATAAATATAGTAACCTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGcc                                                                                                                                                                      >  1:190884/1‑163 (MQ=255)                                                                                                                                                              aaTCAAATTAAGTTACCAATAAGCTCAATTGATTTGATAACTTTAATTTTTGAGATAAATATAGTAACCTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCCGGCGGAGGGATAACGGATTATACGACATTCATTTCGGATGTTGAAAATAATCAGGt                                                                    >  1:36761/1‑251 (MQ=255)                                                                                                                                                                             ccAATAAGCTCAATTGATTTGATAACTTTAATTTTTGAGATAAATATAGTAACCTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCCGGCGGAGGGATAACGGATTATACGACATTCATTTCGGATGTTGAAAATAATCAGGTACGTCAGGCTAAAtt                                                     >  1:387546/1‑251 (MQ=255)                                                                                                                                                                                         aTTGATTTGATAACTTTAATTTTTGAGATAAATATAGTAACCTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCCGGCGGAGGGATAACGGATTATACGACATTCATTTCGGATGTTGAAAATAATCAGGTACGTCAGGCTAAATTTGAAGATAATGa                                         >  1:17795/1‑251 (MQ=255)                                                                                                                                                                                            gatttgatAACTTTAATTTTTGAGATAAATATAGTAACCTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCCGGCGAAGGGATAACGGATTATACGACATTCATTTCGGATGTTGAAAATAATCAGGTACGTCa                                                              >  1:435483/1‑227 (MQ=255)                                                                                                                                                                                                                   gaTAAATATAGTAACCTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCCGGCGGAGGGATAACGGATTATACGACATTCATTTCGGATGTTGAAAATAATCAGGTACGTCAGGCTAAAtt                                                     <  1:422060/213‑1 (MQ=255)                                                                                                                                                                                                                   gaTAAATATAGTAACCTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCCGGCGGAGGGATAACGGATTATACGACATTCATTTCGGATGTTGAAAATAATCAGGTACGTCAGGCTAAATTTGAAGATAATGAAATTTTAGTCACTAAAACCGACGGCa               >  1:477553/1‑251 (MQ=255)                                                                                                                                                                                                                      aaaTATAGTAACCTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAAATTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCCGGCGGAGGGATAACGGATTATACGACATTCATTTCGGATGTTGAAAATAATCAGGTACGTCAGGCTAAATTTGAAGATAATGAAATTTTAGTCACTAAAACCGACGGCACTa            >  1:26601/1‑251 (MQ=255)                                                                                                                                                                                                                        atatAGTAACCTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGcc                                                                                                                                                                      <  1:93219/95‑1 (MQ=255)                                                                                                                                                                                                                         tataGTAACCTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCCGGCGGAGGGATAACGGATTATACGACATTCATTTCGGATGTTGAAAATAATCAGGTACGTCAGGCTAAATTTGAAGATAATGAAATTTTAGTCACTAAAACCGACGGCACTAAat         >  1:582050/1‑251 (MQ=255)                                                                                                                                                                                                                              tAACCTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCCGGCGGAGGGATAACGGATTATACGACATTCATTTCGGATGTTGAAAATAATCAGGTACGTCAGGCTAAATTTGAAGATAATGAAATTTTAGTCACTAAAACCGACGGCACTAAATATac     <  1:41718/250‑1 (MQ=255)                                                                                                                                                                                                                                aCCTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCCGGCGGAGGGATAACGGATTATACGACATTCATTTCGGATGTTGAAAATAATCAGGTACGTCAGGCTAAAtt                                                     >  1:683583/1‑200 (MQ=255)                                                                                                                                                                                                                                aCCTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCCGGCGGAGGGATAACGGATTATACGACATTCATTTCGGATGTTGAAAATAATCAGGTACGTCAGGCTAAATTTGAAGATAATGAAATTTTAGTCACTAAAACCGACGGCACTAAATATacaac  <  1:321059/251‑1 (MQ=255)                                                                                                                                                                                                                                 ccTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCCGGCGGAGGGATAACGGATTATACGACATTCATTTCGGATg                                                                                    >  1:23211/1‑168 (MQ=255)                                                                                                                                                                                                                                 ccTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCCGGCGGAGGGATAACGGATTATACGACATTCATTTCGGATGTTGAAAATAATCAGGTACGTCAGGCt                                                          >  1:858452/1‑194 (MQ=255)                                                                                                                                                                                                                                 ccTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCCGGCGGAGGGATAACGGATTATACGACATTCATTTCGGATGTTGAAAATAATCAGGTACGTCAGGCTAAATTTGAAGATAATGAAATTTTAGTc                               <  1:872061/221‑1 (MQ=255)                                                                                                                                                                                                                                 ccTTACAACATGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCCGGCGGAGGGATAACGGATTATACGACATTCATTTCGGATGTTGAAAATAATCAGGTACGTCAGGCTAAATTTGAAGATAATGAAATTTTAGTCACTAAAACCGACGGCACTAAATATacaac  <  1:169931/250‑1 (MQ=255)                                                                                                                                                                                                                                     acaacaTGTTGTTAACTTATAAATACGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCCGGCGGAGGGATAACGGATTATACGACATTCATTTCGGATGTTGAAAATAATCAGGTACGTCAGGCt                                                          <  1:103624/190‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                AAAGGAAGTTTTGTAGTAAAAGTTTTTCAGGGTGAAGGTTTTGATGAATACTTAAAAGAGATCCGTTCTCTGTTTAGTGTAGTCAAAGTTCGTAAACCTGAAGCCTCCCGAGACCGATCTCGGGAAGTTTATATTGTTGCAAGCGGTTATAAACACTAATCAAATTAAGTTACCAATAAGCTCAATTGATTTGATAACTTTAATTTTTGAGATAAATATAGTAACCTTACAACACGTTGTTAACTTATAAATAAGAGGTAATGCCTTGAACGATATGGTAAAAAATTTAATTCTTTGGGTGGTTGTGGCCATTGTCATGATGACTGCATATCAAAGTTTTAATACCACTTCCGGCGGAGGGATAACGGATTATACGACATTCATTTCGGATGTTGAAAATAATCAGGTACGTCAGGCTAAATTTGAAGATAATGAAATTTTAGTCACTAAAACCGACGGCACTAAATATACAAC  >  1/1141542‑1142015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑