Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,125,556	G→A	intergenic (‑66003/‑6547)	unknown ← / → unknown	tRNA‑Ser‑GGA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,125,556	0	G	A	100.0%	212.0 / NA	78	intergenic (‑66003/‑6547)	unknown/unknown	tRNA‑Ser‑GGA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/46);  total (32/46)

CCGCATTGTTAAAACAAAATAATAACCATAAAATGATGGTATCCGTTTCCCACACCACCAGACAGTCGCGCCCGGGCGAACAAGAAGGCGTGCATTATTATTTTGTTTCTCAAGGGGAATTTGAAACCTTAATCGAACAAAATGCCTTTTTGGAATACGCGAAAGTCTTTGGCGGCAATTATTACGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATGTTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGATGAATTGGAAAGACGCTTAATCGGACGCGGTCAGGACAGTGCGGACGTGATTGCCGACAGAATGGCGAAAGCGATGAGCGAAATTTCTCATTACAATGAATATGATTATGTGATCGTCAACGATAATTTTGAACAGGCGCTTGTGGATTTG  >  1/1125323‑1125798                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                   ccgcATTGTTAAAACAAAATAATAACCATAAAATGATGGTATCCGTTTCCCACACCACCAGACAGTCGCGCCCGGGCGAACAAGAAGGCGTGCATTATTATTTTGTTTCTCAAGGGGAATTTGAAACCTTAATCGAACAAAATGCCTTTTTGGAATACGCGAAAGTCTTTGGCGGCAATTATTACGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATattgat                                                                                                                                                                                                                                   >  1:637949/1‑251 (MQ=255)     aTTGTTAAAACAAAATAATAACCATAAAATGATGGTATCCGTTTCCCACACCACCAGACAGTCGCGCCCGGGCGAACAAGAAGGCGTGCATTATTATTTTGTTTCTCAAGGGGAATTTGAAACCTTAATCGAACAAAATGCCTTTTTGGAATACGCGAAAGTCTTTGGCGGCAATTATTACGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGc                                                                                                                                                                                                                               <  1:744590/251‑1 (MQ=255)      ttGTTAAAACAAAATAATAACCATAAAATGATGGTATCCGTTTCCCACACCACCAGACAGTCGCGCCCGGGCGAACAAGAAGGCGTGCATTATTATTTTGTTTCTCAAGGGGAATTTGAAACCTTAATCGAACAAAATGCCTTTTTGGAATACGCGAAAGTCTTTGGCGGCAATTATTACGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCa                                                                                                                                                                                                                              >  1:291669/1‑251 (MQ=255)       tGTTAAAACAAAATAATAACCATAAAATGATGGTATCCGTTTCCCACACCACCAGACAGTCGCGCCCGGGCGAACAAGAAGGCGTGCATTATTATTTTGTTTCTCAAGGGGAATTTGAAACCTTAATCGAACAAAATGCCTTTTTGGAATACGCGAAAGTCTTTGGCGGCAATTATTACGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATattga                                                                                                                                                                                                                                    >  1:272949/1‑244 (MQ=255)                   ataataACCATAAAATGATGGTATCCGTTTCCCACACCACCAGACAGTCGCGCCCGGGCGAACAAGAAGGCGTGCATTATTATTTTGTTTCTCAAGGGGAATTTGAAACCTTAATCGAACAAAATGCCTTTTTGGAATACGCGAAAGTCTTTGGCGGCAATTATTACGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGat                                                                                                                                                                                                                                         <  1:385669/227‑1 (MQ=255)                      ataaCCATAAAATGATGGTATCCGTTTCCCACACCACCAGACAGTCGCGCCCGGGCGAACAAGAAGGCGTGCATTATTATTTTGTTTCTCAAGGGGAATTTGAAACCTTAATCGAACAAAATGCCTTTTTGGAATACGCGAAAGTCTTTGGCGGCAATTATTACGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAAtt                                                                                                                                                                                                              <  1:665618/251‑1 (MQ=255)                      ataaCCATAAAATGATGGTATCCGTTTCCCACACCACCAGACAGTCGCGCCCGGGCGAACAAGAAGGCGTGCATTATTATTTTGTTTCTCAAGGGGAATTTGAAACCTTAATCGAACAAAATGCCTTTTTGGAATACGCGAAAGTCTTTGGCGGCAATTATTACGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAAtt                                                                                                                                                                                                              <  1:258202/251‑1 (MQ=255)                        aaCCATAAAATGATGGTATCCGTTTCCCACACCACCAGACAGTCGCGCCCGGGCGAACAAGAAGGCGTGCATTATTATTTTGTTTCTCAAGGGGAATTTGAAACCTTAATCGAACAAAATGCCTTTTTGGAATACGCGAAAGTCTTTGGCGGCAATTATTACGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGgcg                                                                                                                                                                                                                         >  1:46322/1‑238 (MQ=255)                          ccATAAAATGATGGTATCCGTTTCCCACACCACCAGACAGTCGCGCCCGGGCGAACAAGAAGGCGTGCATTATTATTTTGTTTCTCAAGGGGAATTTGAAACCTTAATCGAACAAAATGCCTTTTTGGAATACGCGAAAGTCTTTGGCGGCAATTATTACGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCa                                                                                                                                                                                                          >  1:335315/1‑251 (MQ=255)                          ccATAAAATGATGGTATCCGTTTCCCACACCACCAGACAGTCGCGCCCGGGCGAACAAGAAGGCGTGCATTATTATTTTGTTTCTCAAGGGGAATTTGAAACCTTAATCGAACAAAATGCCTTTTTGGAATACGCGAAAGTCTTTGGCGGCAATTATTACGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCa                                                                                                                                                                                                          >  1:814511/1‑251 (MQ=255)                           cATAAAATGATGGTATCCGTTTCCCACACCACCAGACAGTCGCGCCCGGGCGAACAAGAAGGCGTGCATTATTATTTTGTTTCTCAAGGGGAATTTGAAACCTTAATCGAACAAAATGCCTTTTTGGAATACGCGAAAGTCTTTGGCGGCAATTATTACGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCaa                                                                                                                                                                                                         >  1:395346/1‑251 (MQ=255)                               aaaTGATGGTATCCGTTTCCCACACCACCAGACAGTCGCGCCCGGGCGAACAAGAAGGCGTGCATTATTATTTTGTTTCTCAAGGGGAATTTGAAACCTTAATCGAACAAAATGCCTTTTTGGAATACGCGAAAGTCTTTGGCGGCAATTATTACGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCaaaaaa                                                                                                                                                                                                     >  1:250473/1‑251 (MQ=255)                                      ggTATCCGTTTCCCACACCACCAGACAGTCGCGCCCGGGCGAACAAGAAGGCGTGCATTATTATTTTGTTTCTCAAGGGGAATTTGAAACCTTAATCGAACAAAATGCCTTTTTGGAATACGCGAAAGTCTTTGGCGGCAATTATTACGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGc                                                                                                                                                                                                                               <  1:873095/218‑1 (MQ=255)                                           ccGTTTCCCACACCACCAGACAGTCGCGCCCGGGCGAACAAGAAGGCGTGCATTATTATTTTGTTTCTCAAGGGGAATTTGAAACCTTAATCGAACAAAATGCCTTTTTGGAATACGCGAAAGTCTTTGGCGGCAATTATTACGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGgcgc                                                                                                                                                                                                                        >  1:113006/1‑220 (MQ=255)                                           ccGTTTCCCACACCACCAGACAGTCGCGCCCGGGCGAACAAGAAGGCGTGCATTATTATTTTGTTTCTCAAGGGGAATTTGAAACCTTAATCGAACAAAATGCCTTTTTGGAATACGCGAAAGTCTTTGGCGGCAATTATTACGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGtt                                                                                                                                                                                         >  1:292871/1‑251 (MQ=255)                                                tCCCACACCACCAGACAGTCGCGCCCGGGCGAACAAGAAGGCGTGCATTATTATTTTGTTTCTCAAGGGGAATTTGAAACCTTAATCGAACAAAATGCCTTTTTGGAATACGCGAAAGTCTTTGGCGGCAATTATTACGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAagag                                                                                                                                                                                    <  1:637358/251‑1 (MQ=255)                                                       ccaccaGACAGTCGCGCCCGGGCGAACAAGAAGGCGTGCATTATTATTTTGTTTCTCAAGGGGAATTTGAAACCTTAATCGAACAAAATGCCTTTTTGGAATACGCGAAAGTCTTTGGCGGCAATTATTACGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATattga                                                                                                                                                                                                                                    <  1:507454/196‑1 (MQ=255)                                                       ccaccaGACAGTCGCGCCCGGGCGAACAAGAAGGCGTGCATTATTATTTTGTTTCTCAAGGGGAATTTGAAACCTTAATCGAACAAAATGCCTTTTTGGAATACGCGAAAGTCTTTGGCGGCAATTATTACGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTAttttt                                                                                                                                                                             >  1:251271/1‑251 (MQ=255)                                                       ccaccaGACAGTCGCGCCCGGGCGAACAAGAAGGCGTGCATTATTATTTTGTTTCTCAAGGGGAATTTGAAACCTTAATCGAACAAAATGCCTTTTTGGAATACGCGAAAGTCTTTGGCGGCAATTATTACGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTAttttt                                                                                                                                                                             >  1:322949/1‑251 (MQ=255)                                                                 gTCGCGCCCGGGCGAACAAGAAGGCGTGCATTATTATTTTGTTTCTCAAGGGGAATTTGAAACCTTAATCGAACAAAATGCCTTTTTGGAATACGCGAAAGTCTTTGGCGGCAATTATTACGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTAccg                                                                                                                                                                    >  1:739592/1‑250 (MQ=255)                                                                       cccGGGCGAACAAGAAGGCGTGCATTATTATTTTGTTTCTCAAGGGGAATTTGAAACCTTAATCGAACAAAATGCCTTTTTGGAATACGCGAAAGTCTTTGGCGGCAATTATTACGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCcgtcgt                                                                                                                                                             >  1:781736/1‑251 (MQ=255)                                                                            gCGAACAAGAAGGCGTGCATTATTATTTTGTTTCTCAAGGGGAATTTGAAACCTTAATCGAACAAAATGCCTTTTTGGAATACGCGAAAGTCTTTGGCGGCAATTATTACGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGa                                                                                                                                                         <  1:109839/250‑1 (MQ=255)                                                                              gAACAAGAAGGCGTGCATTATTATTTTGTTTCTCAAGGGGAATTTGAAACCTTAATCGAACAAAATGCCTTTTTGGAATACGCGAAAGTCTTTGGCGGCAATTATTACGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGATGa                                                                                                                                                      >  1:703992/1‑251 (MQ=255)                                                                               aaCAAGAAGGCGTGCATTATTATTTTGTTTCTCAAGGGGAATTTGAAACCTTAATCGAACAAAATGCCTTTTTGGAATACGCGAAAGTCTTTGGCGGCAATTATTACGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGATGaa                                                                                                                                                     >  1:328416/1‑251 (MQ=255)                                                                               aaCAAGAAGGCGTGCATTATTATTTTGTTTCTCAAGGGGAATTTGAAACCTCAATCGAACAAAATGCCTTTTTGGAATACGCGAAAGTCTTTGGCGGCAATTATTACGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGt                                                                                                                                                                                   <  1:435036/221‑1 (MQ=255)                                                                                aCAAGAAGGCGTGCATTATTATTTTGTTTCTCAAGGGGAATTTGAAACCTTAATCGAACAAAATGCCTTTTTGGAATACGCGAAAGTCTTTGGCGGCAATTATTACGGCTCGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTAccgc                                                                                                                                                                   <  1:565586/236‑1 (MQ=255)                                                                                 cAAGAAGGCGTGCATTATTATTTTGTTTCTCAAGGGGAATTTGAAACCTTAATCGAACAAAATGCCTTTTTGGAATACGCGAAAGTCTTTGGCGGCAATTATTACGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGATGAAtt                                                                                                                                                   >  1:276172/1‑251 (MQ=255)                                                                                               ttattattTTGTTTCTCAAGGGGAATTTGAAACCTTAATCGAACAAAATGCCTTTTTGGAATACGCGAAAGTCTTTGGCGGCAATTATTACGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAg                                                                                                                                                                                                                             >  1:847275/1‑163 (MQ=255)                                                                                               ttattattTTGTTTCTCAAGGGGAATTTGAAACCTTAATCGAACAAAATGCCTTTTTGGAATACGCGAAAGTCTTTGGCGGCAATTATTACGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTg                                                                                                                                                                            <  1:306318/212‑1 (MQ=255)                                                                                                 attattTTGTTTCTCAAGGGGAATTTGAAACCTTAATCGAACAAAATGCCTTTTTGGAATACGCGAAAGTCTTTGGCGGCAATTATTACGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTg                                                                                                                                                                            >  1:815482/1‑210 (MQ=255)                                                                                                 attattTTGTTTCTCAAGGGGAATTTGAAACCTTAATCGAACAAAATGCCTTTTTGGAATACGCGAAAGTCTTTGGCGGCAATTATTACGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGATGAATTGGAAAGACGCTTAAt                                                                                                                                    >  1:700274/1‑250 (MQ=255)                                                                                                                aaGGGGAATTTGAAACCTTAATCGAACAAAATGCCTTTTTGGAATACGCGAAAGTCTTTGGCGGCAATTATTACGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGATGAATTGGAAAGACGCTTAATCGGACGCGGTCAGGAc                                                                                                                    <  1:45452/251‑1 (MQ=255)                                                                                                                aaGGGGAATTTGAAACCTTAATCGAACAAAATGCCTTTTTGGAATACGCGAAAGTCTTTGGCGGCAATTATTACGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGATGAATTGGAAAGACGCTTAATCGGACGCGGTCAGGAc                                                                                                                    <  1:212118/251‑1 (MQ=255)                                                                                                                aaGGGGAATTTGAAACCTTAATCGAACAAAATGCCTTTTTGGAATACGCGAAAGTCTTTGGCGGCAATTATTACGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGATGAATTGGAAAGACGCTTAATCGGACGCGGTCAGGAc                                                                                                                    <  1:626111/251‑1 (MQ=255)                                                                                                                aaGGGGAATTTGAAACCTTAATCGAACAAAATGCCTTTTTGGAATACGCGAAAGTCTTTGGCGGCAATTATTACGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGATGAATTGGAAAGACGCTTAATCGGACGCGGTCAGGAc                                                                                                                    <  1:191421/251‑1 (MQ=255)                                                                                                                aaGGGGAATTTGAAACCTTAATCGAACAAAATGCCTTTTTGGAATACGCGAAAGTCTTTGGCGGCAATTATTACGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGATGAATTGGAAAGACGCTTAATCGGACGCGGTCAGGAc                                                                                                                    <  1:325852/251‑1 (MQ=255)                                                                                                                aaGGGGAATTTGAAACCTTAATCGAACAAAATGCCTTTTTGGAATACGCGAAAGTCTTTGGCGGCAATTATTACGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGATGAATTGGAAAGACGCTTAATCGGACGCGGTCAGGAc                                                                                                                    <  1:14647/251‑1 (MQ=255)                                                                                                                aaGGGGAATTTGAAACCTTAATCGAACAAAATGCCTTTTTGGAATACGCGAAAGTCTTTGGCGGCAATTATTACGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGATGAATTGGAAAGACGCTTAATCGGACGCGGTCAGGAc                                                                                                                    <  1:374117/251‑1 (MQ=255)                                                                                                                     gAATTTGAAACCTTAATCGAACAAAATGCCTTTTTGGAATACGCGAAAGTCTTTGGCGGCAATTATTACGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGATGAATTGGAAAGACGCTTAATCGGACGCGGTCAGGAc                                                                                                                    >  1:500918/1‑246 (MQ=255)                                                                                                                       aTTTGAAACCTTAATCGAACAAAATGCCTTTTTGGAATACGCGAAAGTCTTTGGCGGCAATTATTACGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTTGATATTGATTTGCAGGGCGCACAACAAATTCGCAAAAAAATGCCGAATGTTTAGAGGATTTTTGTTTTAccgc                                                                                                                                                                   >  1:655372/1‑197 (MQ=255)                                                                                                                          tGAAACCTTAATCGAACAAAATGCCTTTTTGGAATACGCGAAAGTCTTTGGCGGCAATTATTACGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGATGAATTGGAAAGACGCTTAATCGGACGCGGTCAGGACAGTGCGGACg                                                                                                          <  1:833043/251‑1 (MQ=255)                                                                                                                          tGAAACCTTAATCGAACAAAATGCCTTTTTGGAATACGCGAAAGTCTTTGGCGGCAATTATTACGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGATGAATTGGAAAGACGCTTAATCGGACGCGGTCAGGACAGTGCGGACg                                                                                                          <  1:316639/251‑1 (MQ=255)                                                                                                                          tGAAACCTTAATCGAACAAAATGCCTTTTTGGAATACGCGAAAGTCTTTGGCGGCAATTATTACGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGATGAATTGGAAAGACGCTTAATCGGACGCGGTCAGGACAGTGCGGACg                                                                                                          <  1:140239/251‑1 (MQ=255)                                                                                                                               ccTTAATCGAACAAAATGCCTTTTTGGAATACGCGAAAGTCTTTGGCGGCAATTATTACGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGATGAATTGGAAAGACGCTTAATCGGACGCGGTCAGGAc                                                                                                                    <  1:851106/236‑1 (MQ=255)                                                                                                                               ccTTAATCGAACAAAATGCCTTTTTGGAATACGCGAAAGTCTTTGGCGGCAATTATTACGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGATGAATTGGAAAGACGCTTAATCGGACGCGGTCAGGACAGTGCGGACGTGAtt                                                                                                     <  1:20251/251‑1 (MQ=255)                                                                                                                                    aTCGAACAAAATGCCTTTTTGGAATACGCGAAAGTCTTTGGCGGCAATTATTACGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTAtttt                                                                                                                                                                              <  1:733849/173‑1 (MQ=255)                                                                                                                                          cAAAATGCCTTTTTGGAATACGCGAAAGTCTTTGGCGGCAATTATTACGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGATGAATTGGAAAGACGCTTAATCGGACGCGGTCAGGACAGTGCGGACGTGATTGCCGACAGAAt                                                                                          <  1:462286/251‑1 (MQ=255)                                                                                                                                                   tttttGGAATACGCGAAAGTCTTTGGCGGCAATTATTACGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGATGAATTGGAAAGACGCTTAATCGGACGCGGTCAGGAc                                                                                                                    >  1:501876/1‑216 (MQ=255)                                                                                                                                                                                 aaTTATTACGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGATGAATTGGAAAGACGCTTAATCGGACGCGGTCAGGACAGTGCGGACGTGATTGCCGACAGAATGGCGAAAGCGATGAGCGAAATTTCTCATTACAATGAata                                                   <  1:514123/251‑1 (MQ=255)                                                                                                                                                                                     attaCGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGATGAATTGGAAAGACGCTTAATCGGACGCGGTCAGGACAGTGCGGACGTGATTGCCGACAGAATGGCGAAAGCGATGAGCGAAATTTCTCATTACAATGAATATGAt                                               <  1:702921/251‑1 (MQ=255)                                                                                                                                                                                     attaCGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGATGAATTGGAAAGACGCTTAATCGGACGCGGTCAGGACAGTGCGGACGTGATTGCCGACAGAATGGCGAAAGCGATGAGCGAAATTTCTCATTACAATGAATATGAt                                               <  1:850102/251‑1 (MQ=255)                                                                                                                                                                                     attaCGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGATGAATTGGAAAGACGCTTAATCGGACGCGGTCAGGACAGTGCGGACGTGATTGCCGACAGAATGGCGAAAGCGATGAGCGAAATTTCTCATTACAATGAATATGAt                                               <  1:702185/251‑1 (MQ=255)                                                                                                                                                                                     attaCGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGATGAATTGGAAAGACGCTTAATCGGACGCGGTCAGGACAGTGCGGACGTGATTGCCGACAGAATGGCGAAAGCGATGAGCGAAATTTCTCATTACAATGAATATGAt                                               <  1:357507/251‑1 (MQ=255)                                                                                                                                                                                     attaCGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATGCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGATGAATTGGAAAGACGCTTAATCGGACGCGGTCAGGACAGTGCGGACGTGATTGCCGACAGAATGGCGAAAGCGATGAGCGAAATTTCTCATTACAATGAATATGAt                                               <  1:369821/251‑1 (MQ=255)                                                                                                                                                                                         cGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGATGAATTGGAAAGACGCTTAATCGGACGCGGTCAGGACAGTGCGGACGTGATTGCCGACAGAATGGCGAAAGCGATGAGCGAAATTTCTCATTACAATGAATATGATTAtg                                           <  1:456359/251‑1 (MQ=255)                                                                                                                                                                                            cACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGATGAATTGGAAAGACGCTTAATCGGACGCGGTCAGGACAGTGCGGACGTGATTGCCGACAGAATGGCGAAAGCGATGAGCGAAATTTCTCATTACAATGAATATGATTAtgtg                                         <  1:192840/250‑1 (MQ=255)                                                                                                                                                                                            cACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGATGAATTGGAAAGACGCTTAATCGGACGCGGTCAGGACAGTGCGGACGTGATTGCCGACAGAATGGCGAAAGCGATGAGCGAAATTTCTCATTACAATGAATATGATTATGTGa                                        <  1:377365/251‑1 (MQ=255)                                                                                                                                                                                              cGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGATGAATTGGAAAGACGCTTAATCGGACGCGGTCAGGAc                                                                                                                    >  1:220171/1‑173 (MQ=255)                                                                                                                                                                                              cGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGATGAATTGGAAAGACGCTTAATCGGACGCGGTCAGGACAGTGCGGACGTGATTGCCGACAGAATGGCGAAAGCGATGAGCGAAATTTCTCATTACAATGa                                                      >  1:300151/1‑235 (MQ=255)                                                                                                                                                                                              cGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGATGAATTGGAAAGACGCTTAATCGGACGCGGTCAGGACAGTGCGGACGTGATTGCCGACAGAATGGCGAAAGCGATGAGCGAAATTTCTCATTACAATGAATATGATTAtg                                           <  1:751721/246‑1 (MQ=255)                                                                                                                                                                                               gTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGtt                                                                                                                                                                          <  1:117263/118‑1 (MQ=255)                                                                                                                                                                                               gTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGATGAATTGGAAAGACGCTTAATCGGACGCGGTCAGGACAGTGCGGACGTGATTGCCGACAGAATgg                                                                                        >  1:760983/1‑200 (MQ=255)                                                                                                                                                                                                tCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGATGAATTGGAAAGACGCTTAATCGGACGCGGTCAGGACAGTGCGGACGTGATTGCCGAc                                                                                               >  1:682046/1‑192 (MQ=255)                                                                                                                                                                                                tCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGATGAATTGGAAAGACGCTTAATCGGACGCGGTCAGGACAGTGCGGACGTGATTGCCGACAGAATGGCGAAAGCGATGAGCGAAATTTCTCATTACAATGAATATGATTAtgtg                                         >  1:63033/1‑246 (MQ=255)                                                                                                                                                                                                tCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGATGAATTGGAAAGACGCTTAATCGGACGCGGTCAGGACAGTGCGGACGTGATTGCCGACAGAATGGCGAAAGCGATGAGCGAAATTTCTCATTACAATGAATATGATTAtg                                           >  1:259100/1‑244 (MQ=255)                                                                                                                                                                                                   ttATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGATGAATTGGAAAGACGCTTAATCGGACGCGGTCAGGACAGTGCGGACGTGATTGCCGACAGAATGGCGAAAGCGATGAGCGAAATTTCTCATTACAATGAATATGATTATGTGATCGTCaa                                 <  1:241004/251‑1 (MQ=255)                                                                                                                                                                                                    tATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGATGAATTGGAAAGACGCTTAATCGGACGCGGTCAGGACAGTGCGGACGTGATTGCCGACAGAATGGCGAAAGCGATGAGCGAAATTTCTCATTACAATGAATATGATTATGTGATCGTCAAc                                <  1:404801/251‑1 (MQ=255)                                                                                                                                                                                                          cTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGATGAATTGGAAAGACGCTTAATCGGACGCGGTCAGGAc                                                                                                                    >  1:315550/1‑161 (MQ=255)                                                                                                                                                                                                          cTATTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGATGAATTGGAAAGACGCTTAATCGGACGCGGTCAGGACAGTGc                                                                                                               <  1:613946/166‑1 (MQ=255)                                                                                                                                                                                                            aTTGAAGAAAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGATGAATTGGAAAGACGCTTAATCGGACGCGGTCAGGACAGTGCGGACg                                                                                                          <  1:472503/169‑1 (MQ=255)                                                                                                                                                                                                               gaagaaAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGATGAATTGGAAAGACGCTTAATCGGACGCGGTCAGGAc                                                                                                                    >  1:830134/1‑156 (MQ=255)                                                                                                                                                                                                                 agaaAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGATGAATTGGAAAGACGCTTAATCGGACGCGGTCAGGACAGTGCGGACGTGATTGCCGACAGAATGGCGAAAGCGATGAGCGAAATTTCTCATTACAATGAATATGAt                                               <  1:411676/223‑1 (MQ=255)                                                                                                                                                                                                                  gaaAATTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGATGAATTGGAAAGACGCTTAATCGGACGCGGTCAGGACAGTGCGGACGTGATTGCCGACAGAATGGCGAAAGCGATGAGCGAAATTTCTCATTACAATGAATATGATTAtg                                           <  1:146312/226‑1 (MQ=255)                                                                                                                                                                                                                   aaaaTTTAGCTAAAGGCATTGATATTTTTTTGGATATTGATCGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGATGAATTGGAAAGACGCTTAATCGGACGCGGTCAGGACAGTGCGGACGTGATTGCCGACAGAATGGCGAAAGCGATGAGCGAAATTTCTCATTACAATGAATATGATTATGTGATCGTCAACGATAATTTTGAAc                   <  1:881663/249‑1 (MQ=255)                                                                                                                                                                                                                           gCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGATGAATTGGAAAGACGCTTAATCGGACGCGGTCAGGACAGTGCGGACGTGATTGCCGACAGAATGGCGAAAGCGATGAGCGAAATTTCTCATTACAATGAATATGATTAtgt                                          <  1:159947/218‑1 (MQ=255)                                                                                                                                                                                                                           gCTAAAGGCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGATGAATTGGAAAGACGCTTAATCGGACGCGGTCAGGACAGTGCGGACGTGATTGCCGACAGAATGGCGAAAGCGATGAGCGAAATTTCTCATTACAATGAATATGATTATGTGATCGTCAACGATAATTTTGAAc                   <  1:276975/241‑1 (MQ=255)                                                                                                                                                                                                                                  gCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGATGAATTGGAAAGACGCTTAATCGGACGCGGTCAGGAc                                                                                                                    <  1:562095/137‑1 (MQ=255)                                                                                                                                                                                                                                  gCATTGATATTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGATGAATTGGAAAGACGCTTAATCGGACGCGGTCAGGACAGTGCGGACGTGATTGCCGACAGAATGGCGAAAGCGATGAGCGAAATTTCTCATTACAATGAATATGATTATGTGATCGTCAACGATAATTTTGAACAGGCGCTTGTGGATTTg  >  1:335064/1‑251 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                   CCGCATTGTTAAAACAAAATAATAACCATAAAATGATGGTATCCGTTTCCCACACCACCAGACAGTCGCGCCCGGGCGAACAAGAAGGCGTGCATTATTATTTTGTTTCTCAAGGGGAATTTGAAACCTTAATCGAACAAAATGCCTTTTTGGAATACGCGAAAGTCTTTGGCGGCAATTATTACGGCACGTCTTTATTCGCTATTGAAGAAAATTTAGCTAAAGGCATTGATGTTTTTTTGGATATTGATTGGCAGGGCGCACAACAAATTCGCAAAAAAGTGCCGAATGTTAAGAGTATTTTTGTTTTACCGCCGTCGTTGGATGAATTGGAAAGACGCTTAATCGGACGCGGTCAGGACAGTGCGGACGTGATTGCCGACAGAATGGCGAAAGCGATGAGCGAAATTTCTCATTACAATGAATATGATTATGTGATCGTCAACGATAATTTTGAACAGGCGCTTGTGGATTTG  >  1/1125323‑1125798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑