Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,122,770	C→T	intergenic (‑63217/‑9333)	unknown ← / → unknown	tRNA‑Ser‑GGA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,122,770	0	C	T	100.0%	180.0 / NA	66	intergenic (‑63217/‑9333)	unknown/unknown	tRNA‑Ser‑GGA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (37/29);  total (37/29)

CACTTCTTCCGTTTTGGCTAATTCGGTGATGTCGCGTGGTTCAAGCATACCGATAATTTCTGGTAAACCGCGTCGAATTAATAACGGTTTTTTCTGCCAGTAATCGCGTAAAAAGATTTCCGGTGTAATATTTTGAGGTAAACAAAATTTGTTCATATACATTCCTTCAATGTGTTGAATAAGTGTTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCCGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTCACGGGACGGCTGAAAA‑TGCCGATTTTATTTTCGCGTAAATCAATGTCGGTAAATTGTTGTAAAATGCCGGACTGTAAAATGGCGGTTTGCACCATAGTGCCTTCACCTACACTAAACGATTTCAGATAATGATGATCAGGAAACGCATAGGCAATTTGAAT  >  1/1122528‑1122991                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                               cACTTCTTCCGTTTTGGCTAATTCGGTGATGTCGCGTGGTTCAAGCATACCGATAATTTCTGGTAAACCGCGTCGAATTAATAACGGTTTTTTCTGCCAGTAATCGCGTAAAAAGATTTCCGGTGTAATATTTTGAGGTAAACAAAATTTGTTCATATACATTCCTTCAATGTGTTGAATAAGTGTTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAg                                                                                                                                                                                                                        <  1:829328/251‑1 (MQ=255) cACTTCTTCCGTTTTGGCTAATTCGGTGATGTCGCGTGGTTCAAGCATACCGATAATTTCTGGTAAACCGCGTCGAATTAATAACGGTTTTTTCTGCCAGTAATCGCGTAAAAAGATTTCCGGTGTAATATTTTGAGGTAAACAAAATTTGTTCATATACATTCCTTCAATGTGTTGAATAAGTGTTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAg                                                                                                                                                                                                                        >  1:783314/1‑251 (MQ=255) cACTTCTTCCGTTTTGGCTAATTCGGTGATGTCGCGTGGTTCAAGCATACCGATAATTTCTGGTAAACCGCGTCGAATTAATAACGGTTTTTTCTGCCAGTAATCGCGTAAAAAGATTTCCGGTGTAATATTTTGAGGTAAACAAAATTTGTTCATATACATTCCTTCAATGTGTTGAATAAGTGTTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAg                                                                                                                                                                                                                        <  1:727441/251‑1 (MQ=255) cACTTCTTCCGTTTTGGCTAATTCGGTGATGTCGCGTGGTTCAAGCATACCGATAATTTCTGGTAAACCGCGTCGAATTAATAACGGTTTTTTCTGCCAGTAATCGCGTAAAAAGATTTCCGGTGTAATATTTTGAGGTAAACAAAATTTGTTCATATACATTCCTTCAATGTGTTGAATAAGTGTTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAg                                                                                                                                                                                                                        >  1:678246/1‑251 (MQ=255)                ggCTAATTCGGTGATGTCGCGTGGTTCAAGCATACCGATAATTTCTGGTAAACCGCGTCGAATTAATAACGGTTTTTTCTGCCAGTAATCGCGTAAAAAGATTTCCGGTGTAATATTTTGAGGTAAACAAAATTTGTTCATATACATTCCTTCAATGTGTTGAATAAGTATTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCa                                                                                                                                                                                                                            >  1:442269/1‑232 (MQ=255)                                      ggTTCAAGCATACCGATAATTTCTGGTAAACCGCGTCGAATTAATAACGGTTTTTTCTGCCAGTAATCGCGTAAAAAGATTTCCGGTGTAATATTTTGAGGTAAACAAAATTTGTTCATATACATTCCTTCAATGTGTTGAATAAGTGTTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAg                                                                                                                                                                                    >  1:782606/1‑250 (MQ=255)                                       gTTCAAGCATACCGATAATTTCTGGTAAACCGCGTCGAATTAATAACGGTTTTTTCTGCCAGTAATCGCGTAAAAAGATTTCCGGTGTAATATTTTGAGGTAAACAAAATTTGTTCATATACATTCCTTCAATGTGTTGAATAAGTGTTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAg                                                                                                                                                                                    >  1:893181/1‑249 (MQ=255)                                       gTTCAAGCATACCGATAATTTCTGGTAAACCGCGTCGAATTAATAACGGTTTTTTCTGCCAGTAATCGCGTAAAAAGATTTCCGGTGTAATATTTTGAGGTAAACAAAATTTGTTCATATACATTCCTTCAATGTGTTGAATAAGTGTTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAg                                                                                                                                                                                    >  1:519257/1‑249 (MQ=255)                                       gTTCAAGCATACCGATAATTTCTGGTAAACCGCGTCGAATTAATAACGGTTTTTTCTGCCAGTAATCGCGTAAAAAGATTTCCGGTGTAATATTTTGAGGTAAACAAAATTTGTTCATATACATTCCTTCAAAGTGTTGAATAAGTGTTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGc                                                                                                                                                                                   <  1:288748/250‑1 (MQ=255)                                                  ccGATAATTTCTGGTAAACCGCGTCGAATTAATAACGGTTTTTTCTGCCAGTAATCGCGTAAAAAGATTTCCGGTGTAATATTTTGAGGTAAACAAAATTTGTTCATATACATTCCTTCAATGTGTTGAATAAGTGTTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAAt                                                                                                                                                                       >  1:551509/1‑251 (MQ=255)                                                     aTAATTTCTGGTAAACCGCGTCGAATTAATAACGGTTTTTTCTGCCAGTAATCGCGTAAAAAGATTTCCGGTGTAATATTTTGAGGTAAACAAAATTTGTTCATATACATTCCTTCAATGTGTTGAATAAGTGTTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTc                                                                                                                                                                    <  1:272992/251‑1 (MQ=255)                                                              ggTAAACCGCGTCGAATTAATAACGGTTTTTTCTGCCAGTAATCGCGTAAAAAGATTTCCGGTGTAATATTTTGAGGTAAACAAAATTTGTTCATATACATTCCTTCAATGTGTTGAATAAGTGTTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTCACGGGACgg                                                                                                                                                           >  1:871603/1‑251 (MQ=255)                                                                            aaTTAATAACGGTTTTTTCTGCCAGTAATCGCGTAAAAAGATTTCCGGTGTAATATTTTGAGGTAAACAAAATTTGTTCATATACATTCCTTCAATGTGTTGAATAAGGGTTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTCACGGGACGGCTGAAAA‑TGCCGat                                                                                                                                            >  1:669555/1‑251 (MQ=255)                                                                                              cTGCCAGTAATCGCGTAAAAAGATTTCCGGTGTAATATTTTGAGGTAAACAAAATTTGTTCATATACATTCCTTCAATGTGTTGAATAAGTGTTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTCACGGGACGGCTGAAAA‑TGCCGATTTTATTTTCGCGTAAATc                                                                                                                          <  1:604091/251‑1 (MQ=255)                                                                                                gCCAGTAATCGCGTAAAAAGATTTCCGGTGTAATATTTTGAGGTAAACAAAATTTGTTCATATACATTCCTTCAATGTGTTGAATAAGTGTTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAg                                                                                                                                                                                                                        >  1:95049/1‑156 (MQ=255)                                                                                                             tAAAAAGATTTCCGGTGTAATATTTTGAGGTAAACAAAATTTGTTCATATACATTCCTTCAATGTGTTGAATAAGTGTTATTATTCTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTCACGGGACGGCTGAAAA‑TGCCGATTTTATTTTCGCGTAAATCAATGTCGGTAAAttg                                                                                                           <  1:550351/251‑1 (MQ=255)                                                                                                                               aaTATTTTGAGGTAAACAAAATTTGTTCATATACATTCCTTCAATGTGTTGAATAAGTGTTATTATTTTTTCTTTGCTTGATCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTCACGGGACGGCTGAAAA‑TGCCGATTTTATTTTCGCGTAAATCAATGTCGGTAAATTGTTGTAAAATGCCGGACTg                                                                                         <  1:354280/251‑1 (MQ=255)                                                                                                                                  atTTTGAGGTAAACAAAATTTGTTCATATACATTCCTTCAATGTGTTGAATAAGTGTTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTACTTTCACGGGACGGCTGAAAA‑TGCCGATTTTATTTTCGCGTAAATCAATGTCGGTAAATTGTTGTAAAATGCCGGACTGTaa                                                                                      >  1:444851/1‑251 (MQ=255)                                                                                                                                  atTTTGAGGTAAACAAAATTTGTTCATATACATTCCTTCAATGTGTTGAATAAGTGTTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTCACGGGACGGCTGAAAA‑TGCCGattttatt                                                                                                                                      >  1:397019/1‑203 (MQ=255)                                                                                                                                  atTTTGAGGTAAACAAAATTTGTTCATATACATTCCTTCAATGTGTTGAATAAGTGTTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTCACGGGACGGCTGAAAA‑TGCCGATTTTATTTTCGCGTAAATCAATGTCGGTAAATTGTTGTAAAATGCCGGACTGTaa                                                                                      >  1:571521/1‑251 (MQ=255)                                                                                                                                     ttGAGGTAAACAAAATTTGTTCATATACATTCCTTCAATGTGTTGAATAAGTGTTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTCACGGGACGGCTGAAAA‑TGCCGATTTTATTTTCGCGTAAATCAATGTCGGTAAATTGTTGTAAAATGCCGGACTGTAAAAt                                                                                   <  1:183559/251‑1 (MQ=255)                                                                                                                                                  aaTTTGTTCATATACATTCCTTCAATGTGTTGAATAAGTGTTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTCACGGGACGGCTGAAAA‑TGCCGATTTTATTTTCGCGTAAATCAATGTCGGTAAATTGTTGTAAAATGCCGGACTGTAAAATGGCGGTTTGCAc                                                                       >  1:179901/1‑250 (MQ=255)                                                                                                                                                      tGTTCATATACATTCCTTCAATGTGTTGAATAAGTGTTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTCACGGGACGGCTGAAAA‑TGCCGATTTTATTTTCGCGTAAATCAATGTCGGTAAATTGTTGTAAAATGCCGGACTGTAAAATGGCGGTTTGCACCATAg                                                                  >  1:868731/1‑251 (MQ=255)                                                                                                                                                         tCATATACATTCCTTCAATGTGTTGAATAAGTGTTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTCACGGGACGGCTGAAAA‑TGCCGATTTTATTTTCGCGTAAATCAATGTCGGTAAATTGTTGTAAAATGCCGGACTGTAAAATGGCGGTTTGCACCATAGTGc                                                               <  1:796250/251‑1 (MQ=255)                                                                                                                                                           atatACATTCCTTCAATGTGTTGAATAAGTGTTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGc                                                                                                                                                                                   <  1:751990/134‑1 (MQ=255)                                                                                                                                                            tataCATTCCTTCAATGTGTTGAATAAGTGTTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTCACGGGACGGCTGAAAA‑TGCCGATTTTATTTTCGCGTAAATCAATGTCGGTAAATTGTTGTAAAATGCCGGACTGTAAAATGGCGGTTTGCACCATAGTGCCtt                                                            >  1:856708/1‑251 (MQ=255)                                                                                                                                                                cATTCCTTCAATGTGTTGAATAAGTGTTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTCACGGGACGGCTGAAAA‑TGCCGATTTTATTTTCGCGTAAATCAATGTCgg                                                                                                                  >  1:447777/1‑193 (MQ=255)                                                                                                                                                                cATTCCTTCAATGTGTTGAATAAGTGTTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTCACGGGACGGCTGAAAA‑TGCCGATTTTATTTTCGCGTAAATCAATGTCGGTAAATTGTTGTAAAATGCCGGACTGTAAAATGGCGGTTTGCACCATAg                                                                  <  1:360891/241‑1 (MQ=255)                                                                                                                                                                cATTCCTTCAATGTGTTGAATAAGTGTTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTCACGGGACGGCTGAAAA‑TGCCGATTTTATTTTCGCGTAAATCAATGTCGGTAAATTGTTGTAAAATGCCGGACTGTAAAATGGCGGTTTGCACCATAGTGCCTTCAcc                                                        <  1:843351/251‑1 (MQ=255)                                                                                                                                                                    ccTTCAATGTGTTGAATAAGTGTTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAg                                                                                                                                                                                                                        <  1:318746/88‑1 (MQ=255)                                                                                                                                                                    ccTTCAATGTGTTGAATAAGTGTTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTCACGGGACGGCTGAAAA‑TGCCGATTTTATTTTCGCGTAAATCAATGTCGGTa                                                                                                                <  1:7783/191‑1 (MQ=255)                                                                                                                                                                    ccTTCAATGTGTTGAATAAGTGTTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTCACGGGACGGCTGAAAA‑TGCCGATTTTATTTTCGCGTAAATCAATGTCGGTAAATTGTTGTaa                                                                                                     <  1:27653/202‑1 (MQ=255)                                                                                                                                                                    ccTTCAATGTGTTGAATAAGTGTTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTCACGGGACGGCTGAAAA‑TGCCGATTTTATTTTCGCGTAAATCAATGTCGGTAAATTGTTGTAAAATGc                                                                                                >  1:835340/1‑207 (MQ=255)                                                                                                                                                                    ccTTCAATGTGTTGAATAAGTGTTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTCACGGGACGGCTGAAAA‑TGCCGATTTTATTTTCGCGTAAATCAATGTCGGTAAATTGTTGTAAAATGCCGGACTGTAAAATGGCGGTTTGCACCAt                                                                    <  1:335151/235‑1 (MQ=255)                                                                                                                                                                    ccTTCAATGTGTTGAATAAGTGTTATTATTTTTTCTTTGCTTGTTCTTCCGCACGCTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTCACGGGACGGCTGAAAA‑TGCCGATTTTATTTTCGCGTAAATCAATGTCGGTAAATTGTTGTAAAATGCCGGACTGTAAAATGGCGGTTTGCAcc                                                                      >  1:186250/1‑233 (MQ=255)                                                                                                                                                                     cTTCAATGTGTTGAATAAGTGTTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTCACGGGACGGCTGAAAA‑TGCCGATTTTATTTTCGCGTAAATCAATGTCGGTAAATTGTTGTAAAATGCCGGACTGTAAAATGGCGGTTTGCACCATAGTGCCTTCACCTacac                                                   <  1:313597/251‑1 (MQ=255)                                                                                                                                                                     cTTCAATGTGTTGAATAAGTGTTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTCACGGGACGGCTGAAAA‑TGCCGATTTTATTTTCGCGTAAATCAATGTCGGTAAATTGTTGTAAAATGCCGGACTGTAAAATGGCGGTTTGCACCATAGTGCCTTCACCTacac                                                   <  1:687237/251‑1 (MQ=255)                                                                                                                                                                          aTGTGTTGAATAAGTGTTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTCACGGGACGGCTGAAAA‑TGCCGATTTTATTTTCGCGTAAATCAATGTCGGTAAATTGTTGTAAAATGCCGGACTGTAAAATGGCGGTTTGCACCATAGTGCCTTCACCTACACTAAAc                                              <  1:125179/251‑1 (MQ=255)                                                                                                                                                                          aTGTGTTGAATAAGTGTTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTCACGGGACGGCTGAAAA‑TGCCGATTTTATTTTCGCGTAAATCAATGTCGGTAAATTGTTGTAAAATGCCGGACTGTAAAATGGCGGTTTGCACCATAGTGCCTTCACCTACACTAAAc                                              <  1:739649/251‑1 (MQ=255)                                                                                                                                                                            gtgtTGAATAAGTGTTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTa                                                                                                                                                                         >  1:423672/1‑127 (MQ=255)                                                                                                                                                                            gtgtTGAATAAGTGTTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTCACGGGACGGCTGAAAA‑TGCCGATTTTATTTTCGCGTAAATCAATGTCGGTAAATTGTTGTAAAATGCCGGACTGTAAAATGGCGGTTTGCACCATAGTGCCTTCACCTACACTAAAc                                              <  1:300647/249‑1 (MQ=255)                                                                                                                                                                            gtgtTGAATAAGTGTTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTCACGGGACGGCTGAAAA‑TGCCGATTTTATTTTCGCGTAAATCAATGTCGGTAAATTGTTGTAAAATGCCGGACTGTAAAATGGCGGTTTGCACCATAGTGCCTTCACCTACACTAAACGa                                            >  1:330387/1‑251 (MQ=255)                                                                                                                                                                            gtgtTGAATAAGTGTTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTCACGGGACGGCTGAAAA‑TGCCGATTTTATTTTCGCGTAAATCAATGTCGGTAAATTGTTGTAAAATGCCGGACTGTAAAATGGCGGTTTGCACCATAGTGCCTTCACCTACACTAAACGa                                            >  1:875366/1‑251 (MQ=255)                                                                                                                                                                              gtTGAATAAGTGTTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTCACGGGACGGCTGAAAA‑TGCCGATTTTATTTTCGCGTAAATCAATGTCGGTAAATTGTTGTAAAATGCCGGACTGTAAAATGGCGGTTTGCACCATAGTGCCTTCACCTACACTAAACGAtt                                          >  1:386094/1‑251 (MQ=255)                                                                                                                                                                                 gAATAAGTGTTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGc                                                                                                                                                                                   >  1:802965/1‑112 (MQ=255)                                                                                                                                                                                 gAATAAGTGTTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTCACGGGACGGCTGaaa                                                                                                                                                     <  1:613104/142‑1 (MQ=255)                                                                                                                                                                                 gAATAAGTGTTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTCACGGGACGGCTGAAAA‑TGCCGATTTTATTTTCGCGTAAATCAATGTCGGTAAATTGTTGTAAAATGCCGGACTGTAAAATGGCGGTTTGCACCATAg                                                                  >  1:412389/1‑224 (MQ=255)                                                                                                                                                                                 gAATAAGTGTTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTCACGGGACGGCTGAAAA‑TGCCGATTTTATTTTCGCGTAAATCAATGTCGGTAAATTGTTGTAAAATGCCGGACTGTAAAATGGCGGTTTGCACCATAGTGCCTTCACCTACACTAAACGATTTCa                                       >  1:42784/1‑251 (MQ=255)                                                                                                                                                                                 gAATAAGTGTTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTCACGGGACGGCTGAAAA‑TGCCGATTTTATTTTCGCGTAAATCAATGTCGGTAAATTGTTGTAAAATGCCGGACTGTAAAATGGCGGTTTGCACCATAGTGCCTTCACCTACACTAAACGATTTCa                                       >  1:411138/1‑251 (MQ=255)                                                                                                                                                                                 gAATAAGTGTTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTCACGGGACGGCTGAAAA‑TGCCGATTTTATTTTCGCGTAAATCAATGTCGGTAAATTGTTGTAAAATGCCGGACTGTAAAATGGCGGTTTGCACCATAGTGCCTTCACCTACACTAAACGATTTCa                                       >  1:680476/1‑251 (MQ=255)                                                                                                                                                                                       gtgtTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTCACGGGACGGCTGAAAATTGCCGATTTTATTTTCGCGTAAATCAATGTCGGTAAATTGTTGTAAAATGCCGGACTGTAAAATGGCGGTTTGCACCATAGTGc                                                               <  1:256505/222‑1 (MQ=255)                                                                                                                                                                                       gtgtTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTCACGGGACGGCTGAAAA‑TGCCGATTTTATTTTCGCGTAAATCAATGTCGGTAAAt                                                                                                             <  1:486880/175‑1 (MQ=255)                                                                                                                                                                                       gtgtTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTCACGGGACGGCTGAAAA‑TGCCGATTTTATTTTCGCGTAAATCAATGTCGGTAAATTGTTGTAAAATGCCGGACTGTAAAATGGCGGTTTGCACCATAGTGCCTTCACCTACACTAAACGATTTCAGATAat                                 >  1:681524/1‑251 (MQ=255)                                                                                                                                                                                       gtgtTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTCACGGGACGGCTGAAAA‑TGCCGATTTTATTTTCGCGTAAATCAATGTCGGTAAATTGTTGTAAAATGCCGGACTGTAAAATGGCGGTTTGCACCATAGTGCCTTCACCTACACTAAACGATTTCAGATAat                                 >  1:529116/1‑251 (MQ=255)                                                                                                                                                                                          ttattaTTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGc                                                                                                                                                                                   <  1:541351/103‑1 (MQ=255)                                                                                                                                                                                          ttattaTTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTCACGGGACGGCTGAAAA‑TGCCGATTTTATTTTCGCGTAAATCAATGTCGGTAAATTGTTGTAAAATGCCGGACTGTAAAATGGCGGTTTGCACCATAGTGCCTTCACCTACACTAAACGATTTCAGATAatga                               <  1:400964/250‑1 (MQ=255)                                                                                                                                                                                              tattTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTCACGGGACGGCTGAAAA‑TGCCGATTTTATTTTCGCGTAAATCAATGTCGGTAAATTGTTGTAAAATGCCGGACTGTAAAATGGCGGTTTGCACCATAGTGCCTTCACCTACACTAAACGATTTCAGATAATgatgat                           >  1:864096/1‑250 (MQ=255)                                                                                                                                                                                                         tGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTCACGGGACGGCTGAAAA‑TGCCGATTTTATTTTCGCGTAAATCAATGTCGGTAAAttgttg                                                                                                        >  1:824535/1‑162 (MQ=255)                                                                                                                                                                                                         tGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTCACGGGACGGCTGAAAA‑TGCCGATTTTATTTTCGCGTAAATCAATGTCGGTAAATTGTTGTAAAATGCCGGACTGTAAAATGGCGGTTTGCACCATAGTGCCTTCACCTACACTAAACGATTTCAGATAATGATGATCAGGAAACGCAt               >  1:888123/1‑251 (MQ=255)                                                                                                                                                                                                          gCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTCACGGGACGGCTGAAAA‑TGCCGATTTTATTTTCGCGTAAATCAATGTCGGTAAATTGTTGTAAAATGCCGGACTGTAAAATGGCGGTTTGCACCATAGTGCCTTCACCTACACTAAACGATTTCAGATAATGATGATCAgg                       >  1:826217/1‑242 (MQ=255)                                                                                                                                                                                                          gCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTCACGGGACGGCTGAAAA‑TGCCGATTTTATTTTCGCGTAAATCAATGTCGGTAAATTGTTGTAAAATGCCGGACTGTAAAATGGCGGTTTGCACCATAGTGCCTTCACCTACACTAAACGATTTCAGATAATGATGATCAGGAAACGCATa              >  1:35407/1‑251 (MQ=255)                                                                                                                                                                                                          gCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTCACGGGACGGCTGAAAA‑TGCCGATTTTATTTTCGCGTAAATCAATGTCGGTAAATTGTTGTAAAATGCCGGACTGTAAAATGGCGGTTTGCACCATAGTGCCTTCACCTACACTAAACGATTTCAGATAATGATGATCAGGAAACGCATa              >  1:594488/1‑251 (MQ=255)                                                                                                                                                                                                            ttGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTCACGGGACGGCTGAAAA‑TGCCGATTTTATTTTCGCGTAAATCAATGTCGGTAAATTGTTGTAAAATGCCGGACTGTAAAATGGCGGTTTGCACCATAGTGCCTTCACCTACACTAAACGATTTCAGATAATGATGATCAGGAAACGCATAgg            >  1:460370/1‑251 (MQ=255)                                                                                                                                                                                                             tGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTCACGGGACGGCTGAAAA‑TGCCGATTTTATTTTCGCGTAAATCAATGTCGGTAAATTGTTGTAAAATGCCGGACTGTAAAATGGCGGTTTGCACCATAGTGCCTTCACCTACACTAAAc                                              <  1:497112/216‑1 (MQ=255)                                                                                                                                                                                                             tGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTCACGGGACGGCTGAAAA‑TGCCGATTTTATTTTCGCGTAAATCAATGTCGGTAAATTGTTGTAAAATGCCGGACTGTAAAATGGCGGTTTGCACCATAGTGCCTTCACCTACACTAAACGATTTCAGATAATGATGATCAGGAAACGCATAGGc           <  1:838172/251‑1 (MQ=255)                                                                                                                                                                                                                         cGTTTGCGTCGAATTTCTTTCGGATCTGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTCACGGGACGGCTGAAAA‑TGCCGATTTTATTTTCGCGTAAATCAATGTCGGTAAATTGTTGTAAAATGCCGGACTGTAAAATGGCGGTTTGCACCATAGTGCCTTCACCTACACTAAACGATTTCAGATAATGATGATCAGGAAACGCATAGGCAATTTGAat  <  1:53044/248‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                               CACTTCTTCCGTTTTGGCTAATTCGGTGATGTCGCGTGGTTCAAGCATACCGATAATTTCTGGTAAACCGCGTCGAATTAATAACGGTTTTTTCTGCCAGTAATCGCGTAAAAAGATTTCCGGTGTAATATTTTGAGGTAAACAAAATTTGTTCATATACATTCCTTCAATGTGTTGAATAAGTGTTATTATTTTTTCTTTGCTTGTTCTTCCGCACGTTTGCGTCGAATTTCTTTCGGATCCGCCAACAGCGGGCGATAAATTTCAATGCGATCGCCGTCTTTCAGCTGATCCGTTAATTTCACGGGACGGCTGAAAA‑TGCCGATTTTATTTTCGCGTAAATCAATGTCGGTAAATTGTTGTAAAATGCCGGACTGTAAAATGGCGGTTTGCACCATAGTGCCTTCACCTACACTAAACGATTTCAGATAATGATGATCAGGAAACGCATAGGCAATTTGAAT  >  1/1122528‑1122991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑