Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,121,746	C→A	intergenic (‑62193/‑10357)	unknown ← / → unknown	tRNA‑Ser‑GGA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,121,746	0	C	A	100.0%	200.0 / NA	73	intergenic (‑62193/‑10357)	unknown/unknown	tRNA‑Ser‑GGA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (31/42);  total (31/42)

TAACAACGCCCAGCCGTCGTCCAGCCAATTGCAAATGCTATCGACCAACACTTCCATGGAAATCTGTTGATCCTGCAGGCTGCCGATTAAGCGGGTTAAAAAAGCAAAATCAACGGTTTGACCATCGGCAAGTTGTTTCAAAATTTCCGCTTCGGCGTAGGTCAATTCATCCAGCCATTCGCCGTTGGCATAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATCTGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGCTGACGGTTGTTGCAAGCGCTTGTTGGAATAAGGTGTCGAACTGTGCAGAATTTGCCAGCTGTTGTAAAAATAACGCTTTCATTTGTTGTACGTCTTGCGGTGTCAATTCGCCGGTTTTTTGTTCTGAAGGACTTAAACGGAACGGGATAGCCAGTTCATTCA  >  1/1121499‑1121989                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    tAACAACGCCCAGCCGTCGTCCAGCCAATTGCAAATGCTATCGACCAACACTTCCATGGAAATCTGTTGATCCTGCAGGCTGCCGATTAAGCGGGTTAAAAAAGCAAAATCAACGGTTTGACCATCGGCAAGTTGTTTCAAAATTTCCGCTTCGGCGTAGGTCAATTCATCCAGCCATTCGCCGTTGGCATAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATgc                                                                                                                                                                                                                                                  <  1:381372/251‑1 (MQ=255)  aacaacGCCCAGCCGTCGTCCAGCCAATTGCAAATGCTATCGACCAACACTTCCATGGAAATCTGTTGATCCTGCAGGCTGCCGATTAAGCGGGTTAAAAAAGCAAAATCAACGGTTTGACCATCGGCAAGTTGTTTCAAAATTTCCGCTTCGGCGTAGGTCAATTCATCCAGCCATTCGCCGTTGGCATAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATgcc                                                                                                                                                                                                                                                 <  1:529734/251‑1 (MQ=255)      acGCCCAGCCGTCGTCCAGCCAATTGCAAATGCTATCGACCAACACTTCCATGGAAATCTGTTGATCCTGCAGGCTGCCGATTAAGCGGGTTAAAAAAGCAAAATCAACGGTTTGACCATCGGCAAGTTGTTTCAAAATTTCCGCTTCGGCGTAGGTCAATTCATCCAGCCATTCGCCGTTGGCATAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCa                                                                                                                                                                                                                                             <  1:266541/251‑1 (MQ=255)                 tcgtcCAGCCAATTGCAAATGCTATCGACCAACACTTCCATGGAAATCTGTTGATCCTGCAGGCTGCCGATTAAGCGGGTTAAAAAAGCAAAATCAACGGTTTGACCATCGGCAAGTTGTTTCAAAATTTCCGCTTCGGCGTAGGTCAATTCATCCAGCCATTCGCCGTTGGCATAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGc                                                                                                                                                                                                                                   <  1:466059/250‑1 (MQ=255)                 tcgtcCAGCCAATTGCAAATGCTATCGACCAACACTTCCATGGAAATCTGTTGATCCTGCAGGCTGCCGATTAAGCGGGTTAAAAAAGCAAAATCAACGGTTTGACCATCGGCAAGTTGTTTCAAAATTTCCGCTTCGGCGTAGGTCAATTCATCCAGCCATTCGCCGTTGGCATAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGc                                                                                                                                                                                                                                   <  1:873723/250‑1 (MQ=255)                   gtcCAGCCAATTGCAAATGCTATCGACCAACACTTCCATGGAAATCTGTTGATCCTGCAGGCTGCCGATTAAGCGGGTTAAAAAAGCAAAATCAACGGTTTGACCATCGGCAAGTTGTTTCAAAATTTCCGCTTCGGCGTAGGTCAATTCATCCAGCCATTCGCCGTTGGCATAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCttt                                                                                                                                                                                                                                >  1:382506/1‑251 (MQ=255)                          cAATTGCAAATGCTATCGACCAACACTTCCATGGAAATCTGTTGATCCTGCAGGCTGCCGATTAAGCGGGTTAAAAAAGCAAAATCAACGGTTTGACCATCGGCAAGTTGTTTCAAAATTTCCGCTTCGGCGTAGGTCAATTCATCCAGCCATTCGCCGTTGGCATAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAacac                                                                                                                                                                                                                         <  1:674315/251‑1 (MQ=255)                          cAATTGCAAATGCTATCGACCAACACTTCCATGGAAATCTGTTGATCCTGCAGGCTGCCGATTAAGCGGGTTAAAAAAGCAAAATCAACGGTTTGACCATCGGCAAGTTGTTTCAAAATTTCCGCTTCGGCGTAGGTCAATTCATCCAGCCATTCGCCGTTGGCATAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAacac                                                                                                                                                                                                                         <  1:81750/251‑1 (MQ=255)                            aTTGCAAATGCTATCGACCAACACTTCCATGGAAATCTGTTGATCCTGCAGGCTGCCGATTAAGCGGGTTAAAAAAGCAAAATCAACGGTTTGACCATCGGCAAGTTGTTTCAAAATTTCCGCTTCGGCGTAGGTCAATTCATCCAGCCATTCGCCGTTGGCATAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGt                                                                                                                                                                                                                       <  1:381450/251‑1 (MQ=255)                                   aTGCTATCGACCAACACTTCCATGGAAATCTGTTGATCCTGCAGGCTGCCGATTAAGCGGGTTAAAAAAGCAAAATCAACGGTTTGACCATCGGCAAGTTGTTTCAAAATTTCCGCTTCGGCGTAGGTCAATTCATCCAGCCATTCGCCGTTGGCATAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCgg                                                                                                                                                                                                                <  1:28272/251‑1 (MQ=255)                                    tGCTATCGACCAACACTTCCATGGAAATCTGTTGATCCTGCAGGCTGCCGATTAAGCGGGTTAAAAAAGCAAAATCAACGGTTTGACCATCGGCAAGTTGTTTCAAAATTTCCGCTTCGGCGTAGGTCAATTCATCCAGCCATTCGCCGTTGGCATAAATTCGGAACGGATCTCGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCaa                                                                                                                                                                                                                            <  1:557990/238‑1 (MQ=255)                                      cTATCGACCAACACTTCCATGGAAATCTGTTGATCCTGCAGGCTGCCGATTAAGCGGGTTAAAAAAGCAAAATCAACGGTTTGACCATCGGCAAGTTGTTTCAAAATTTCCGCTTCGGCGTAGGTCAATTCATCCAGCCATTCGCCGTTGGCATAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGAt                                                                                                                                                                                                              <  1:412918/250‑1 (MQ=255)                                               aaCACTTCCATGGAAATCTGTTGATCCTGCAGGCTGCCGATTAAGCGGGTTAAAAAAGCAAAATCAACGGTTTGACCATCGGCAAGTTGTTTCAAAATTTCCGCTTCGGCGTAGGTCAATTCATCCAGCCATTCGCCGTTGGCATAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAAcc                                                                                                                                                                                                    <  1:700040/251‑1 (MQ=255)                                                   cTTCCATGGAAATCTGTTGATCCTGCAGGCTGCCGATTAAGCGGGTTAAAAAAGCAAAATCAACGGTTTGACCATCGGCAAGTTGTTTCAAAATTTCCGCTTCGGCGTAGGTCAATTCATCCAGCCATTCGCCGTTGGCATAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATgccgcc                                                                                                                                                                                                                                              >  1:325151/1‑205 (MQ=255)                                                            aaaTCTGTTGATCCTGCAGGCTGCCGATTAAGCGGGTTAAAAAAGCAAAATCAACGGTTTGACCATCGGCAAGTTGTTTCAAAATTTCCGCTTCGGCGTAGGTCAATTCATCCAGCCATTCGCCGTTGGCATAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGt                                                                                                                                                                                       <  1:408491/251‑1 (MQ=255)                                                              aTCTGTTGATCCTGCAGGCTGCCGATTAAGCGGGTTAAAAAAGCAAAATCAACGGTTTGACCATCGGCAAGTTGTTTCAAAATTTCCGCTTCGGCGTAGGTCAATTCATCCAGCCATTCGCCGTTGGCATAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTaa                                                                                                                                                                                     <  1:188880/251‑1 (MQ=255)                                                                 tGTTGATCCTGCAGGCTGCCGATTAAGCGGGTTAAAAAAGCAAAATCAACGGTTTGACCATCGGCAAGTTGTTTCAAAATTTCCGCTTCGGCGTAGGTCAATTCATCCAGCCATTCGCCGTTGGCATAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTc                                                                                                                                                                                  <  1:673495/251‑1 (MQ=255)                                                                      aTCCTGCAGGCTGCCGATTAAGCGGGTTAAAAAAGCAAAATCAACGGTTTGACCATCGGCAAGTTGTTTCAAAATTTCCGCTTCGGCGTAGGTCAATTCATCCAGCCATTCGCCGTTGGCATAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTc                                                                                                                                                                                                                                        <  1:722576/192‑1 (MQ=255)                                                                      aTCCTGCAGGCTGCCGATTAAGCGGGTTAAAAAAGCAAAATCAACGGTTTGACCATCGGCAAGTTGTTTCAAAATTTCCGCTTCGGCGTAGGTCAATTCATCCAGCCATTCGCCGTTGGCATAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAgc                                                                                                                                                                             <  1:33377/251‑1 (MQ=255)                                                                      aTCCTGCAGGCTGCCGATTAAGCGGGTTAAAAAAGCAAAATCAACGGTTTGACCATCGGCAAGTTGTTTCAAAATTTCCGCTTCGGCGTAGGTCAATTCATCCAGCCATTCGCCGTTGGCATAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAg                                                                                                                                                                              >  1:438150/1‑250 (MQ=255)                                                                                cTGCCGATTAAGCGGGTTAAAAAAGCAAAATCAACGGTTTGACCATCGGCAAGTTGTTTCAAAATTTCCGCTTCGGCGTAGGTCAATTCATCCAGCCATTCGCCGTTGGCATAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGc                                                                                                                                                                    <  1:508953/250‑1 (MQ=255)                                                                                     gATTAAGCGGGTTAAAAAAGCAAAATCAACGGTTTGACCATCGGCAAGTTGTTTCAAAATTTCCGCTTCGGCGTAGGTCAATTCATCCAGCCATTCGCCGTTGGCATAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGCTGACgg                                                                                                                                                              >  1:596957/1‑251 (MQ=255)                                                                                      aTTAAGCGGGTTAAAAAAGCAAAATCAACGGTTTGACCATCGGCAAGTTGTTTCAAAATTTCCGCTTCGGCGTAGGTCAATTCATCCAGCCATTCGCCGTTGGCATAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTATTATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGCTGACgg                                                                                                                                                              <  1:418221/250‑1 (MQ=255)                                                                                      aTTAAGCGGGTTAAAAAAGCAAAATCAACGGTTTGACCATCGGCAAGTTGTTTCAAAATTTCCGCTTCGGCGTAGGTCAATTCATCCAGCCATTCGCCGTTGGCATAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACg                                                                                                                                                                      >  1:676777/1‑242 (MQ=255)                                                                                                       aGCAAAATCAACGGTTTGACCATCGGCAAGTTGTTTCAAAATTTCCGCTTCGGCGTAGGTCAATTCATCCAGCCATTCGCCGTTGGCATAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGCTGACGGTTGTTGCAAGCGCttgtt                                                                                                                                            >  1:35653/1‑251 (MQ=255)                                                                                                            aaTCAACGGTTTGACCATCGGCAAGTTGTTTCAAAATTTCCGCTTCGGCGTAGGTCAATTCATCCAGCCATTCGCCGTTGGCATAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCAt                                                                                                                                                                                <  1:173293/210‑1 (MQ=255)                                                                                                            aaTCAACGGTTTGACCATCGGCAAGTTGTTTCAAAATTTCCGCTTCGGCGTAGGTCAATTCATCCAGCCATTCGCCGTTGGCATAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGCCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGCTGACGGTTGTTGCAAGCGCTTATTGGAAt                                                                                                                                       >  1:157245/1‑251 (MQ=255)                                                                                                                aaCGGTTTGACCATCGGCAAGTTGTTTCAAAATTTCCGCTTCGGCGTAGGTCAATTCATCCAGCCATTCGCCGTTGGCATAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGCTGACGGTTGTTGCAAGCGCTTGTTGGAATAAgg                                                                                                                                   <  1:31797/251‑1 (MQ=255)                                                                                                                aaCGGTTTGACCATCGGCAAGTTGTTTCAAAATTTCCGCTTCGGCGTAGGTCAATTCATCCAGCCATTCGCCGTTGGCATAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGCTGACGGTTGTTGCAAGCGCTTGTTGGAATAAgg                                                                                                                                   <  1:887874/251‑1 (MQ=255)                                                                                                                 aCGGTTTGACCATCGGCAAGTTGTTTCAAAATTTCCGCTTCGGCGTAGGTCAATTCATCCAGCCATTCGCCGTTGGCATAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGCTGACGGTTGTTGCAAGCGCTTGTTGGAATAAGgt                                                                                                                                  <  1:535619/251‑1 (MQ=255)                                                                                                                  cGGTTTGACCATCGGCAAGTTGTTTCAAAATTTCCGCTTCGGCGTAGGTCAATTCATCCAGCCATTCGCCGTTGGCATAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGCTGACGGTTGTTGCAAGCGCTTGTTGGAATAAGgtg                                                                                                                                 >  1:175331/1‑251 (MQ=255)                                                                                                                    gTTTGACCATCGGCAAGTTGTTTCAAAATTTCCGCTTCGGCGTAGGTCAATTCATCCAGCCATTCGCCGTTGGCATAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATc                                                                                                                                                                                                             <  1:18013/173‑1 (MQ=255)                                                                                                                    gTTTGACCATCGGCAAGTTGTTTCAAAATTTCCGCTTCGGCGTAGGTCAATTCATCCAGCCATTCGCCGTTGGCATAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGCTGACGGTTGTTGCAAGCGCTTGTTGGAATAAGgtgt                                                                                                                                >  1:280395/1‑250 (MQ=255)                                                                                                                         aCCATCGGCAAGTTGTTTCAAAATTTCCGCTTCGGCGTAGGTCAATTCATCCAGCCATTCGCCGTTGGCATAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGCTGACGGTTGTTGCAAGCGCTTGTTGGAATAAGGTGTCGAAc                                                                                                                           <  1:280142/250‑1 (MQ=255)                                                                                                                         aCCATCGGCAAGTTGTTTCAAAATTTCCGCTTCGGCGTAGGTCAATTCATCCAGCCATTCGCCGTTGGCATAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGCTGACGGTTGTTGCAAGCGCTTGTTGGAATAAGGTGTCGAAc                                                                                                                           <  1:188227/250‑1 (MQ=255)                                                                                                                            aTCGGCAAGTTGTTTCAAAATTTCCGCTTCGGCGTAGGTCAATTCATCCAGCCATTCGCCGTTGGCATAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGCTGACGGTTGTTGCAAGCGCTTGTTGGAATAAGGTGTCGAACtgtg                                                                                                                       >  1:40797/1‑251 (MQ=255)                                                                                                                              cGGCAAGTTGTTTCAAAATTTCCGCTTCGGCGTAGGTCAATTCATCCAGCCATTCGCCGTTGGCATAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGCTGACGGTTGTTGCAAGCGCTTGTTGGAATAAGGTGTCGAAc                                                                                                                           >  1:719047/1‑245 (MQ=255)                                                                                                                               ggCAAGTTGTTTCAAAATTTCCGCTTCGGCGTAGGTCAATTCATCCAGCCATTCGCCGTTGGCATAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGCTGACGGTTGTTGCAAGCGCTTGTTGGAATAAGGTGTCGAACTGTGCAg                                                                                                                    >  1:595856/1‑251 (MQ=255)                                                                                                                                  aaGTTGTTTCAAAATTTCCGCTTCGGCGTAGGTCAATTCATCCAGCCATTCGCCGTTGGCATAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGCTGACGGTTGTTGCAAGCGCTTGTTGGAATAAGGTGTCGAACTGTGCAGAAt                                                                                                                 <  1:702614/251‑1 (MQ=255)                                                                                                                                            aaaaTTTCCGCTTCGGCGTAGGTCAATTCATCCAGCCATTCGCCGTTGGCATAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGCTGACGGTTGTTGCAAGCGCTTGTTGGAATAAGGTGTCGAACTGTGCAGAATTTGCCAGCtg                                                                                                       <  1:507440/251‑1 (MQ=255)                                                                                                                                              aaTTTCCGCTTCGGCGTAGGTCAATTCATCCAGCCATTCGCCGTTGGCATAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGCTGACGGTTGTTGCAAGCGCTTGTTGGAATAAGGTGTCGAACTGTGCAGAATTTGCCAGCtgtt                                                                                                     >  1:30877/1‑251 (MQ=255)                                                                                                                                                      cTTCGGCGTAGGTCAATTCATCCAGCCATTCGCCGTTGGCATAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGCTGACGGTTGTTGCAAGCGCTTGTTGGAATAAGGTGTCGAACTGTGCAGAATTTGCCAGCTGTTGTAAAAAt                                                                                             >  1:142489/1‑251 (MQ=255)                                                                                                                                                           gCGTAGGTCAATTCATCCAGCCATTCGCCGTTGGCATAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGCTGACGGTTGTTGCAAGCGCTTGTTGGAATAAGGTGTCGAACTGTGCAGAATTTGCCAGCtg                                                                                                       >  1:285651/1‑236 (MQ=255)                                                                                                                                                            cGTAGGTCAATTCATCCAGCCATTCGCCGTTGGCATAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGCTGACGGTTGTTGCAAGCGCTTGTTGGAATAAGGTGTCGAACTGTGCAGAATTTGCCAGCTGTTGTAAAAATAACGc                                                                                        <  1:387667/250‑1 (MQ=255)                                                                                                                                                                ggTCAATTCATCCAGCCATTCGCCGTTGGCATAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGCTGACGGTTGTTGCAAGCGCTTGTTGGAATAAGGTGTCGAAc                                                                                                                           >  1:817337/1‑211 (MQ=255)                                                                                                                                                                ggTCAATTCATCCAGCCATTCGCCGTTGGCATAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGCTGACGGTTGTTGCAAGCGCTTGTTGGAATAAGGTGTCGAACTGTGCAGAATTTGCCAGCTGTTGTAAAAATAACGCTTTCa                                                                                   >  1:564760/1‑251 (MQ=255)                                                                                                                                                                        cATCCAGCCATTCGCCGTTGGCATAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGCTGACGGTTGTTGCAAGCGCTTGTTGGAATAAGGTGTCGAACTGTGCAGAATTTGCCAGCTGTTGTAAAAATAACGCTTTCATTtgttgt                                                                           >  1:479584/1‑251 (MQ=255)                                                                                                                                                                               ccATTCGCCGTTGGCATAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACGCGTCTTCCGGATCGGAAAAACCGACGAATTCCAGTAATACATAGCGCCGACGGCTGACGGTTGTTGCAAGCGCTTGTTGGAATAAGGTGTCGAACTGTGCAGAATTTGCCAGCTGTTGTAGAAATAACGCTTTCATTTGTTGTACGt                                                                       <  1:232771/248‑1 (MQ=255)                                                                                                                                                                                   tCGCCGTTGGCATAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGCTGACGGTTGTTGCAAGCGCTTGTTGGAATAAGGTGTCGAACTGTGCAGAATTTGCCAGCTGTTGTAAAAATAACGCTTTCATTTGTTGTACGTCTTGCgg                                                                <  1:834558/251‑1 (MQ=255)                                                                                                                                                                                              aTAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGCTGACGGTTGTTGCAAGCGCTTGTTGGAATAAGGTGTCGAACTGTGCAGAATTTGCCAGCTGTTGTAAAAATAACGCTTTCATTTGTTGTACGTCTTGCGGTGTCAATTCGc                                                     <  1:314113/251‑1 (MQ=255)                                                                                                                                                                                                            gATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGCTGACGGTTGTTGCAAGCGCTTGTTGGAATAAGGTGTCGAACTGTGCAGAATTTGCCAGCTGTTGTAAAAATAACGCTTTCATTTGTTGTACGTCTTGCGGTGTCAATTCGCCGGTTTTTTg                                           >  1:463081/1‑247 (MQ=255)                                                                                                                                                                                                            gATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGCTGACGGTTGTTGCAAGCGCTTGTTGGAATAAGGTGTCGAACTGTGCAGAATTTGCCAGCTGTTGTAAAAATAACGCTTTCATTTGTTGTACGTCTTGCGGTGTCAATTCGCCGGTTTTTTGtt                                         >  1:13286/1‑249 (MQ=255)                                                                                                                                                                                                            gATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGCTGACGGTTGTTGCAAGCGCTCGTTGGAATAAGGTGTCGAACTGTGCAGAATTTGCCAGCTGTTGTAAAAATAACGCTTTCATTTGTTGTACGTCTTGCGGTGTCAATTCGCCGGTTTTTTGtt                                         >  1:107924/1‑249 (MQ=255)                                                                                                                                                                                                               cTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGCTGACGGTTGTTGCAAGCGCTTGTTGGAATAAGGTGTCGAACTGTGCAGAATTTGCCAGCTGTTGTAAAAATaa                                                                                           <  1:652297/196‑1 (MQ=255)                                                                                                                                                                                                                   ggTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGCTGACGGTTGTTGCAAGCGCTTGTTGGAATAAGGTGTCGAACTGTGCAGAATTTGCCAGCTGTTGTAAAAATAACGCTTTCATTTGTTGTACGt                                                                       >  1:759548/1‑212 (MQ=255)                                                                                                                                                                                                                   ggTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGCTGACGGTTGTTGCAAGCGCTTGTTGGAATAAGGTGTCGAACTGTGCAGAATTTGCCAGCTGTTGTAAAAATAACGCTTTCATTTGTTGTACGTCTTGCGGTGTCAATTCGCCGGTTTTTTGTTCTGAAGGa                                 >  1:546869/1‑250 (MQ=255)                                                                                                                                                                                                                    gTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGCTGACGGTTGTTGCAAGCGCTTGTTGGAATAAGGTGTCGAACTGTGCAGAATTTGCCAGCTGTTGTaaaaa                                                                                              >  1:102500/1‑188 (MQ=255)                                                                                                                                                                                                                    gTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGCTGACGGTTGTTGCAAGCGCTTGTTGGAATAAGGTGTCGAACTGTGCAGAATTTGCCAGCTGTTGTaaaaa                                                                                              <  1:548107/188‑1 (MQ=255)                                                                                                                                                                                                                    gTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGCTGACGGTTGTTGCAAGCGCTTGTTGGAATAAGGTGTCGAACTGTGCAGAATTTGCCAGCTGTTGTaaaa                                                                                               >  1:231499/1‑187 (MQ=255)                                                                                                                                                                                                                    gTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGCTGACGGTTGTTGCAAGCGCTTGTTGGAATAAGGTGTCGAACTGTGCAGAATTTGCCAGCTGTTGTAAAAATAACGCTTTCATTTGTTGTACGTCTTGCGGTGTCAATTCGCCGGTTTTTTGTTCTGAAGGa                                 >  1:26371/1‑249 (MQ=255)                                                                                                                                                                                                                    gTATAAACCAATTTGCAATTGTTGTCTTGACGTAATATGCCACCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGCTGACGGTTGTTGCAAGCGCTTGTTGGAATAAGGTGTCGAACTGTGCAGAATTTGCCAGCTGTTGTAAAAATAACGCTTTCATTTGTTGTACGTCTTGCgg                                                                >  1:895510/1‑218 (MQ=255)                                                                                                                                                                                                                          aCCAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGCTGACGGTTGTTGCAAGCGCTTGTTGGAATAAGGTGTCGAACTGTGCAGAATTTGCCAGCTGTTGTAAAAATAACGCTTTCATTTGTTGTACGTCTTGCGGTGTCAATTCGCCGGTTTTTTGTTCTGAAGGACTTAaacg                         <  1:838071/251‑1 (MQ=255)                                                                                                                                                                                                                           ccAATTTGCAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGCTGACGGTTGTTGCAAGCGCTTGTTGGAATAAGGTGTCGAACTGTGCAGAATTTGCCAGc                                                                                                         <  1:184866/170‑1 (MQ=255)                                                                                                                                                                                                                                   cAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGCTGACGGTTGTTGCAAGCGCTTGTTGGAATAAGGTGTCGAACTGTGCAGAATTTGCCAGCTGTTGTAAAAATAACGCTTTCATTTGTTGTACGTCTTGCGGTGTCAATTCGCCGGTTTTTTGTTCTGAAGGACTTAAACGGAACGGGAt                <  1:64455/251‑1 (MQ=255)                                                                                                                                                                                                                                   cAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGCTGACGGTTGTTGCAAGCGCTTGTTGGAATAAGGTGTCGAACTGTGCAGAATTTGCCAGCTGTTGTAAAAATAACGCTTTCATTTGTTGTACGTCTTGCGGTGTCAATTCGCCGGTTTTTTGTTCTGAAGGACTTAAACGGAACGGGAt                <  1:580129/251‑1 (MQ=255)                                                                                                                                                                                                                                   cAATTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGCTGACGGTTGTTGCAAGCGCTTGTTGGAATAAGGTGTCGAACTGTGCAGAATTTGCCAGCTGTTGTAAAAATAACGCTTTCATTTGTTGTACGTCTTGCGGTGTCAATTCGCCGGTTTTTTGTTCTGAAGGACTTAAACGGAACGGGAt                <  1:437055/251‑1 (MQ=255)                                                                                                                                                                                                                                     aTTGTTGTCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGCTGACGGTTGTTGCAAGCGCTTGTTGGAATAAGGTGTCGAAc                                                                                                                           <  1:872102/142‑1 (MQ=255)                                                                                                                                                                                                                                       tgttgtCTTTACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGCTGACGGTTGTTGCAAGCGCTTGTTGGAATAAGGTGTCGAACTGTGCAGAATTTGCCAGCTGTTGTAAAAATAACGCTTTCATTTGTTGTACGTCTTGCGGTGTCAATTCGCCGGTTTTTTGTTCTGAAGGACTTAAACGGAACGGGATAGcc            <  1:359330/251‑1 (MQ=255)                                                                                                                                                                                                                                        gttgtCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGCTGACGGTTGTTGCAAGCGCTTGTTGGAATAAGGTGTCGAACtg                                                                                                                         <  1:706627/141‑1 (MQ=255)                                                                                                                                                                                                                                        gttgtCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGCTGACGGTTGTTGCAAGCGCTTGTTGGAATAAGGTGTCGAACTGTGCAGAATTTGCCAGCTGTTGTaaaaa                                                                                              >  1:404871/1‑168 (MQ=255)                                                                                                                                                                                                                                           gtCTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGCTGACGGTTGTTGCAAGCGCTTGTTGGAATAAGGTGTCGAACTGTGCAGAATTTGCCAGCTGTTGTAAAAATAACGCTTTCATTTGTTGTACGTCTTGCGGTGTCAATTCGCCGGTTTTTTGTTCTGAAGGACTTAAACGGAACGGGATAGCCAGtt        >  1:854667/1‑251 (MQ=255)                                                                                                                                                                                                                                             cTTGACGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGCTGACGGTTGTTGCAAGCGCTTGTTGGAATAAGGTGTCGAACTGTGCAGAATTTGCCAGCTGTTGTAAAAATAACGCTTTCATTTGTTGTACGTCTTGCGGTGTCAATTCGCCGGTTTTTTGTTCTGAAGGACTTAAACGGAACGGGATAGcc            >  1:474950/1‑245 (MQ=255)                                                                                                                                                                                                                                                 aCGTAATATGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGCTGACGGTTGTTGCAAGCGCTTGTTGGAATAAGGTGTCGAACTGTGCAGAATTTGCCAGCTGTTGTAAAAATAACGCTTTCATTTGTTGTACGTCTTGCGGTGTCAATTCGCCGGTTTTTTGTTCTGAAGGACTTAAACGGAACGGGATAGCCAGttcattca  >  1:17176/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    TAACAACGCCCAGCCGTCGTCCAGCCAATTGCAAATGCTATCGACCAACACTTCCATGGAAATCTGTTGATCCTGCAGGCTGCCGATTAAGCGGGTTAAAAAAGCAAAATCAACGGTTTGACCATCGGCAAGTTGTTTCAAAATTTCCGCTTCGGCGTAGGTCAATTCATCCAGCCATTCGCCGTTGGCATAAATTCGGAACGGATCTTGGGTATAAACCAATTTGCAATTGTTGTCTTGACGTAATCTGCCGCCATGTTCAAAGCTTTCCAACACGTCTTCCGGATCGGAAAAACCGGCGACTTCCAGTAATTCATAGCGCCGACGGCTGACGGTTGTTGCAAGCGCTTGTTGGAATAAGGTGTCGAACTGTGCAGAATTTGCCAGCTGTTGTAAAAATAACGCTTTCATTTGTTGTACGTCTTGCGGTGTCAATTCGCCGGTTTTTTGTTCTGAAGGACTTAAACGGAACGGGATAGCCAGTTCATTCA  >  1/1121499‑1121989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑