Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,111,950	G→A	intergenic (‑52397/‑20153)	unknown ← / → unknown	tRNA‑Ser‑GGA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,111,950	0	G	A	100.0%	55.1 / NA	22	intergenic (‑52397/‑20153)	unknown/unknown	tRNA‑Ser‑GGA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/8);  total (14/8)

ATACAAAGAAGCTTTAATTGGCAAATCTCCTCTAACTAACTGTACCAGTAATAATCCCATTTCTTACAGCTACGATTCCCACCCACTGTAAATTTGCTTAAGTGTTTTCATTTATCCCCCAACACAAAAATAAACAGGGAGCTTTCAATCTTTCCAAAGTTATATCTACTAAATGAATATTGTAGCCCCGACTACAATTAATAATAATTCGAAAGATAAAAACAATTATAAACAGATTTCTTGGGTAATTTTAATTTATTCTATTTCTTCCGAAAAACGTTCAATACGAGCGCCTAAGCCGCGGAGCTTTTCTTCAATATGTTCATAGCCGCGGTCGATATGGTAGATACGATCCACAATGGTTTCACCGGTGGCGATACAGCCTGCCAGCACTAAGCTGATAGAAGCGCGCAGATCCGTTGCCATGACTTCTGCACCGGATAAATGGGAAACCCCATGGCAAATGGCGGTGTTG  >  1/1111706‑1112180                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                       aTACAAAGAAGCTTTAATTGGCAAATCTCCTCTAACTAACTGTACCAGTAATAATCCCATTTCTTACAGCTACGATTCCCACCCACTGTAAATTTGCTTAAGTGTTTTCATTTATCCCCCAACACAAAAATAAACAGGGAGCTTTCAATCTTTCCAAAGTTATATCTACTAAATGAATATTGTAGCCCCGACTACAATTAATAATAATTCGAAAGATAAAAACAATTATAAACAGATTTCTTGGAtaattt                                                                                                                                                                                                                                  >  1:443153/1‑251 (MQ=255)    cAAAGAAGCTTTAATTGGCAAATCTCCTCTAACTAACTGTACCAGTAATAATCCCATTTCTTACAGCTACGATTCCCACCCACTGTAAATTTGCTTAAGTGTTTTCATTTATCCCCCAACACAAAAATAAACAGGGAGCTTTCAATCTTTCCAAAGTTATATCTACTAAATGAATATTGTAGCCCCGACTACAATTAATAATAATTCGAAAGATAAAAACAATTATAAACAGATTTCTTGGAtaattttaa                                                                                                                                                                                                                               >  1:891421/1‑251 (MQ=255)                                                          cATTTCTTACAGCTACGATTCCCACCCACTGTAAATTTGCTTAAGTGTTTTCATTTATCCCCCAACACAAAAATAAACAGGGAGCTTTCAATCTTTCCAAAGTTATATCTACTAAATGAATATTGTAGCCCCGACTACAATTAATAATAATTCGAAAGATAAAAACAATTATAAACAGATTTCTTGGATAATTTTAATTTATTCTATTTCTTCCGAAAAACGTTCAATACGAGCGCCTAAGCCGCGGAg                                                                                                                                                                           >  1:870818/1‑249 (MQ=255)                                                                            ttCCCACCCACTGTAAATTTGCTTAAGTGTTTTCATTTATCCCCCAACACAAAAATAAACAGGGAGCTTTCAATCTTTCCAAAGTTATATCTACTAAATGAATATTGTAGCCCCGACTACAATTAATAATAATTCGAAAGATAAAAACAATTATAAACAGATTTCTTGGATAATTTTAATTTATTCTATTTCTTCCGAAAAACGTTCAATACGAGCGCCTAAGCCGCGGAGCTTTTCTTCAATATGTTCAt                                                                                                                                                       >  1:156716/1‑251 (MQ=255)                                                                                                                                     gaCAGGGAGCTTTCAATCTTTCCAAAGTTATATCTACTAAATGAATATTGTAGCCCCGACTACAATTAATAATAATTCGAAAGATAAAAACAATTATAAACAGATTTCTTGGATAATTTTAATTTATTCTATTTCTTCCGAAAAACGTTCAATACGAGCGCCTAAGCCGCGGAGCTTTTCTTCAATATGTTCATAGCCGCGGTCGATATGGTAGAtc                                                                                                                                >  1:253170/2‑216 (MQ=255)                                                                                                                                             gCTTTCAATCTTTCCAAAGTTATATCTACTAAATGAATATTGTAGCCCCGACTACAATTAATAATAATTCGAAAGATAAAAACAATTATAAACAGATTTCTTGGATAATTTTAATTTATTCTATTTCTTCCGAAAAACGTTCAATACGAGCGCCTAAGCCGCGGAGCTTTTCTTCAATATGTTCATAGCCGCGGTCGATATGGTAGATACGATCCACAATGGTTTCACCGGTGGCGATACAGCCTGcc                                                                                         >  1:633372/1‑248 (MQ=255)                                                                                                                                                        ttCCAAAGTTATATCTACTAAATGAATATTGTAGCCCCGACTACAATTAATAATAATTCGAAAGATAAAAACAATTATAAACAGATTTCTTGGATAATTTTAATTTATTCTATTTCTTCCGAAAAACGTTCAATACGAGCGCCTAAGCCGCGGAGCTTTTCTTCAATATGTTCATAGCCGCGGTCGATATgg                                                                                                                                      <  1:844244/192‑1 (MQ=255)                                                                                                                                                           cAAAGTTATATCTACTAAATGAATATTGTAGCCCCGACTACAATTAATAATAATTCGAAAGATAAAAACAATTATAAACAGATTTCTTGGATAATTTTAATTTATTCTATTTCTTCCGAAAAACGTTCAATACGAGCGCCTAAGCCGCGGAGCTTTTCTTCAATATGTTCATAGCCGCGGTCGATATGGTAGATACGATCCACAATGGTTTCACCGGTGGCGATACAGCCTGCCAGCACTAAGCTGATAGa                                                                        <  1:477490/251‑1 (MQ=255)                                                                                                                                                             aaGTTATATCTACTAAATGAATATTGTAGCCCCGACTACAATTAATAATAATTCGAAAGATAAAAACAATTATAAACAGATTTCTTGGATAATTTTAATTTATTCTATTTCTTCCGAAAAACGTTCAATACGAGCGCCTAAGCCGCGGAGCTTTTCTTCAATATGTTCATAGCCGCGGTCGATATGGTAGATACGATCCACAATGGTTTCACCGGTGGCGATACAGCCTGCCAGCACTAAGCTGATAGAAg                                                                      <  1:788973/251‑1 (MQ=255)                                                                                                                                                               gTTATATCTACTAAATGAATATTGTAGCCCCGACTACAATTAATAATAATTCGAAAGATAAAAACAATTATAAACAGATTTCTTGGATAATTTTAATTTATTCTATTTCTTCCGAAAAACGTTCAATACGAGCGCCTAAGCCGCGGAGCTTTTCTTCAATATGTTCATAGCCGCGGTCGATATGGTAGATACGATCCACAATGGTTTCACCGGTGGCGATACAGCCTGCCAGCACTAAGCTGATAg                                                                         <  1:742987/246‑1 (MQ=255)                                                                                                                                                                           aaaTGAATATTGTAGCCCCGACTACAATTAATAATAATTCGAAAGATAAAAACAATTATAAACAGATTTCTTGGATAATTTTAATTTATTCTATTTCTTCCGAAAAACGTTCAATACGAGCGCCTAAGCCGCGGAGCTTTTCTTCAATATGTTCATAGCCGCGGTCGATATGGTAGATACGATCCACAAt                                                                                                                     >  1:179010/1‑190 (MQ=255)                                                                                                                                                                               gAATATTGTAGCCCCGACTACAATTAATAATAATTCGAAAGATAAAAACAATTATAAACAGATTTCTTGGATAATTTTAATTTATTCTATTTCTTCCGAAAAACGTTCAATACGAGCGCCTAAGCCGCGGAGCTTTTCTTCAATATGTTCATAGCCGCGGTCGATATGGTAGATACGATCCACAATGGTTTCACCGGTGGCGATACAGCCTGCCAGCACTAAGCTGATAGAAGCGCGCAGATCCGTTGCCa                                                    >  1:447175/1‑251 (MQ=255)                                                                                                                                                                                    ttGTAGCCCCGACTACAATTAATAATAATTCGAAAGATAAAAACAATTATAAACAGATTTCTTGGATAATTTTAATTTATTCTATTTCTTCCGAAAAACGTTCAATACGAGCGCCTAAGCCGCGGAGCTTTTCTTCAATATGTTCATAGCCGCGGTCGATATGGTAGATACGATCCACAATGGTTTCACCGGTGGCGATACAGCCTGCCAGCACTAAGCTGATAGAAGCGCGCAGATCCGTTGCCATGa                                                 >  1:565666/1‑249 (MQ=255)                                                                                                                                                                                    ttGTAGCCCCGACTACAATTAATAATAATTCGAAAGATAAAAACAATTATAAACAGATTTCTTGGATAATTTTAATTTATTCTATTTCTTCCGAAAAACGTTCAATACGAGCGCCTAAGCCGCGGAGCTTTTCTTCAATATGTTCATAGCCGCGGTCGATATGGTAGATACGATCCACAATGGTTTCACCGGTGGCGATACAGCCTGCCAGCACTAAGCTGATAGAAGCGCGCAGATCCGTTGCCATGACt                                               <  1:236014/251‑1 (MQ=255)                                                                                                                                                                                       tAGCCCCGACTACAATTAATAATAATTCGAAAGATAAAAACAATTATAAACAGATTTCTTGGATAATTTTAATTTATTCTATTTCTTCCGAAAAACGTTCAATACGAGCGCCTAAGCCGCGGAGCTTTTCTTCAATATGTTCATAGCCGCGGTCGATATGGTAGATACGATCCACAATGGTTTCACCGGTGGCGGTACAGCCTGCCAGCACTAAGCTGATAGAAGCGCGCAGATCCGTTGCCATGACTTc                                             <  1:481272/250‑1 (MQ=255)                                                                                                                                                                                                  aCAATTAATAATAATTCGAAAGATAAAAACAATTATAAACAGATTTCTTGGATAATTTTAATTTATTCTATTTCTTCCGAAAAACGTTCAATACGAGCGCCTAAGCCGCGGAGCTTTTCTTCAATATGTTCATAGCCGCGGTCGATATGGTAGATACGATCCACAATGGTTTCACCGGTGGCGATACAGCCTGCCAGCACTAAGCTGATAGa                                                                        >  1:748588/1‑212 (MQ=255)                                                                                                                                                                                                                               cAATTATAAACAGATTTCTTGGATAATTTTAATTTATTCTATTTCTTCCGAAAAACGTTCAATACGAGCGCCTAAGCCGCGGAGCTTTTCTTCAATATGTTCATAGCCGCGGTCGATATGGTAGATACGATCCACAATGGTTTCAcc                                                                                                            <  1:169416/147‑1 (MQ=255)                                                                                                                                                                                                                                 aTTATAAACAGATTTCTTGGATAATTTTAATTTATTCTATTTCTTCCGAAAAACGTTCAATACGAGCGCCTAAGCCGCGGAGCTTTTCTTCAATATGTTCATAGCCGCGGTCGATATGGTAGATACGATCCACAATGGTTTCACCGGTGGCGATACAGCCTGCCAGCACTAAGCTGATAGAAGCGCGCAGATCCGTTGCCATGACTTCTGCACCGGATAAATg                              >  1:173187/1‑223 (MQ=255)                                                                                                                                                                                                                                 aTTATAAACAGATTTCTTGGATAATTTTAATTTATTCTATTTCTTCCGAAAAACGTTCAATACGAGCGCCTAAGCCGCGGAGCTTTTCTTCAATATGTTCATAGCCGCGGTCGATATGGTAGATACGATCCACAATGGTTTCACCGGTGGCGATACAGCCTGCCAGCACTAAGCTGATAGAAGCGCGCAGATCCGTTGCCATGACTTCTGCACCGGATAAATGGGAAACCCCATGGc                >  1:276125/1‑237 (MQ=255)                                                                                                                                                                                                                                 aTTATAAACAGATTTCTTGGATAATTTTAATTTATTCTATTTCTTCCGAAAAACGTTCAATACGAGCGCCTAAGCCGCGGAGCTTTTCTTCAATATGTTCATAGCCGCGGTCGATATGGTAGATACGATCCACAATGGTTTCACCGGTGGCGATACAGCCTGCCAGCACTAAGCTGATAGAAGCGCGCAGATCCGTTGCCATGACTTCTGCACCGGATAAATGGGAAACCCCATGGCAAATGGCGGTGTTg  >  1:36757/1‑251 (MQ=255)                                                                                                                                                                                                                                 aTTATAAACAGATTTCTTGGATAATTTTAATTTATTCTATTTCTTCCGAAAAACGTTCAATACGAGCGCCTAAGCCGCGGAGCTTTTCTTCAATATGTTCATAGCCGCGGTCGATATGGTAGATACGATCCACAATGGTTTCACCGGTGGCGATACAGCCTGCCAGCACTAAGCTGATAGAAGCGCGCAGATCCGTTGCCATGACTTCTGCACCGGATAAATGGGAAACCCCATGGCAAATGGCGGTGTTg  >  1:245897/1‑251 (MQ=255)                                                                                                                                                                                                                                                cTTGGATAATTTTAATTTATTCTATTTCTTCCGAAAAACGTTCAATACGAGCGCCTAAGCCGCGGAGCTTTTCTTCAATATGTTCATAGCCGCGGTCGATATGGTAGATACGATCCACAATGGTTTCACCGGTGGCGATACAGCCTGCCAGCACTAAGCTGATAGa                                                                        <  1:765551/166‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                       ATACAAAGAAGCTTTAATTGGCAAATCTCCTCTAACTAACTGTACCAGTAATAATCCCATTTCTTACAGCTACGATTCCCACCCACTGTAAATTTGCTTAAGTGTTTTCATTTATCCCCCAACACAAAAATAAACAGGGAGCTTTCAATCTTTCCAAAGTTATATCTACTAAATGAATATTGTAGCCCCGACTACAATTAATAATAATTCGAAAGATAAAAACAATTATAAACAGATTTCTTGGGTAATTTTAATTTATTCTATTTCTTCCGAAAAACGTTCAATACGAGCGCCTAAGCCGCGGAGCTTTTCTTCAATATGTTCATAGCCGCGGTCGATATGGTAGATACGATCCACAATGGTTTCACCGGTGGCGATACAGCCTGCCAGCACTAAGCTGATAGAAGCGCGCAGATCCGTTGCCATGACTTCTGCACCGGATAAATGGGAAACCCCATGGCAAATGGCGGTGTTG  >  1/1111706‑1112180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑