Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,109,367	C→T	intergenic (‑49814/‑22736)	unknown ← / → unknown	tRNA‑Ser‑GGA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,109,367	0	C	T	100.0%	32.4 / NA	14	intergenic (‑49814/‑22736)	unknown/unknown	tRNA‑Ser‑GGA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/10);  total (4/10)

ATTTTTCTGAATTAAGAAATTTTGCTCTGGAATGTTCTGGCTATTCGTGGGTATTTTTTATAGATGCTGATGAGTGGGTTGATGGCAGAGCGTACAGAGACCTTCGAGACTTTCTTATTATGTATGGTAGATGCAACGACGCAACAGTTTTTTGCCCTAGGTTTTTGGATTCTAAAAATAATAAAAATTATGGTGTTGGGAGAATATTCAAAAATAATGGACTCATTAAATACAATGGAAGAGCGCATGAATACCCATATTCTAAAAATAACATAGTTAATATACCTATAAAAATTGATAGAAATATAAGGCTATTAGAGATAAATCAAATAGAGGATCCTTATAATATAAGATGGAAGTTTTTTCTCG  >  1/1109124‑1109492                                                                                                                                                                                                                                                    |                                                                                                                              attcttCTGAATTAAGAAATTTTGCTCTGGAATGTTCTGGCTATTCGTGGGTATTTTTTATAGATGCTGATGAGTGGGTTGATGGCAGAGCGTACAGAGACCTTCGAGACTTTCTTATTATGTATGGTAGATGCAACGACGCAACAGTTTTTTGCCCTAGGTTTTTGGATTCTAAAAATAATAAAAATTATGGTGTTGGGAGAATATTCAAAAATAATGGACTCATTAAATACAATGGAAGAGTGCATGaa                                                                                                                        <  1:430814/247‑1 (MQ=255) aTTTTTCTGAATTAAGAAATTTTGCTCTGGAATGTTCTGGCTATTCGTGGGTATTTTTTATAGATGCTGATGAGTGGGTTGATGGCAGAGCGTACAGAGACCTTCGAGACTTTCTTATTATGTATGGTAGATGCAACGACGCAACAGTTTTTTGCCCTAGGTTTTTGGATTCTAAAAATAATAAAAATTATGGTGTTGGGAGAATATTCAAAAATAATGGACTCATTAAATACAATGGAAGAGTGCATGaa                                                                                                                        <  1:314288/251‑1 (MQ=255)            ttAAGAAATTTTGCTCTGGAATGTTCTGGCTATTCGTGGGTATTTTTTATAGATGCTGATGAGTGGGTTGATGGCAGAGCGGACAGAGACCTTCGAGACTTTCTTATTGTGTATGGTAGATGCAACGACGCAACAGTTTTTTGCCCTAGGTTTTTGGATTCTAAAAATAATAAAAATTATGGTGTTGGGAGAATATTCAAAAATAATGGACTCATTAAATACAATGGAAGAGTGCATGAATACCCATATTc                                                                                                             <  1:446835/251‑1 (MQ=255)                                  gTTCTGGCTATTCGTGGGTATTTTTTATAGATGCTGATGAGTGGGTTGATGGCAGAGCGTACAGAGACCTTCGAGACTTTCTTATTATGTATGGTAGATGCAACGACGCAACAGTTTTTTGCCCTAGGTTTTTGGATTCTAAAAATAATAAAAATTATGGTGTTGGGAGAATATTCAAAAATAATGGACTCATTAAATACAATGGAAGAGTGCATGAATACCCATATTCTAAAAATAACAt                                                                                                 <  1:584146/241‑1 (MQ=255)                                       ggCTATTCGTGGGTATTTTTTATAGATGCTGATGAGTGGGTTGATGGCAGAGCGTACAGAGACCTTCGAGACTTTCTTATTATGTATGGTAGATGCAACGACGCAACAGTTTTTTGCCCTAGGTTTTTGGATTCTAAAAATAATAAAAATTATGGTGTTGGGAGAATATTCAAAAATAATGGACTCATTAAATACAATGGAAGAGTGCATGAATACCCATATTCTAAAAATAACATAGTTAATATAc                                                                                      <  1:763280/247‑1 (MQ=255)                                                        ttttATAGATGCTGATGAGTGGGTTGATGGCAGAGCGTACAGAGACCTTCGAGACTTTCTTATTATGTATGGTAGATGCAACGACGCAACAGTTTTTTGCCCTAGGTTTTTGGATTCTAAAAATAATAAAAATTATGGTGTTGGGAGAATATTCAAAAATAATGGACTCATTAAATACAATGGAAGAGTGCATGAATACCCATATTCTAAAAATAACATAGTTAATATAc                                                                                      <  1:364142/230‑1 (MQ=255)                                                                         aGTGGGTTGATGGCAGAGCGTACAGAGACCTTCGAGACTTTCTTATTATGTATGGTAGATGCAACGACGCAACAGTTTTTTGCCCTAGGTTTTTGGATTCTAAAAATAATAAAAATTATGGTGTTGGGAGAATATTCAAAAATAATGGACTCATTAAATACAATGGAAGAGTGCATGAATACCCATATTCTAAAAATAACATAGTTAATATACCTATAAAAATTGATAGAAATATAAGGCTATTAGAGAt                                                 <  1:676206/250‑1 (MQ=255)                                                                              gTTGATGGCAGAGCGTACAGAGACCTTCGAGACTTTCTTATTATGTATGGTAGATGCAACGACGCAACAGTTTTTTGCCCTAGGTTTTTGGATTCTAAAAATAATAAAAATTATGGTGTTGGGAGAATATTCAAAAATAATGGACTCATTAAATACAATGGAAGAGTGCATGAATACCCATATTCTAAAAATAACATAGTTAATATAcc                                                                                     <  1:231494/209‑1 (MQ=255)                                                                                          gCGTACAGAGACCTTCGAGACTTTCTTATTATGTATGGTAGATGCAACGACGCAACAGTTTTTTGCCCTAGGTTTTTGGATTCTAAAAATAATAAAAATTATGGTGTTGGGAGAATATTCAAAAATAATGGACTCATTAAATACAATGGAAGAGTGCATGAATACCCATATTCTAAAAATAACATAGTTAATATACCTATAAAAATTGATAGAAATATAAGGCTATTag                                                     <  1:49440/229‑1 (MQ=255)                                                                                                                        atgtatgGTAGATGCAACGACGCAACAGTTTTTTGCCCTAGGTTTTTGGATTCTAAAAATAATAAAAATTATGGTGTTGGGAGAATATTCAAAAATAATGGACTCATTAAATACAATGGAAGAGTGCATGAATACCCATATTCTAAAAATAACATAGTTAATATACCTATAAAAATTGATAGAAATATAAGGCTATTAGAGATAAATCAAATAGAGGATCCTTATAATATAAGATGGAAGTTTTTTCTCg  >  1:867352/1‑250 (MQ=255)                                                                                                                          gtatgGTAGATGCAACGACGCAACAGTTTTTTGCCCTAGGTTTTTGGATTCTAAAAATAATAAAAATTATGGTGTTGGGAGAATATTCAAAAATAATGGACTCATTAAATACAATGGAAGAGTGCATGAATACCCATATTCTAAAAATAACATAGTTAATATAc                                                                                      <  1:827990/164‑1 (MQ=255)                                                                                                                                                                                                 gtgtTGGGAGAATATTCAAAAATAATGGACTCATTAAATACAATGGAAGAGTGCATGAATACCCATATTCTAAAAATAACATAGTTAATATAc                                                                                      >  1:450967/1‑93 (MQ=255)                                                                                                                                                                                                                              cTCATTAAATACAATGGAAGAGTGCATGAATACCCATATTCTAAAAATAACATAGTTAATATAc                                                                                      >  1:255800/1‑64 (MQ=255)                                                                                                                                                                                                                                           aTGGAAGAGTGCATGAATACCCATATTCTAAAAATAACATAGTTAATATACCTATAAAAATTGATAGAAATATAAGGCTATTAGAGATAAATCAAATAGAGGATCCTta                            >  1:117674/1‑109 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                              ATTTTTCTGAATTAAGAAATTTTGCTCTGGAATGTTCTGGCTATTCGTGGGTATTTTTTATAGATGCTGATGAGTGGGTTGATGGCAGAGCGTACAGAGACCTTCGAGACTTTCTTATTATGTATGGTAGATGCAACGACGCAACAGTTTTTTGCCCTAGGTTTTTGGATTCTAAAAATAATAAAAATTATGGTGTTGGGAGAATATTCAAAAATAATGGACTCATTAAATACAATGGAAGAGCGCATGAATACCCATATTCTAAAAATAACATAGTTAATATACCTATAAAAATTGATAGAAATATAAGGCTATTAGAGATAAATCAAATAGAGGATCCTTATAATATAAGATGGAAGTTTTTTCTCG  >  1/1109124‑1109492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑