Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,105,703	C→T	intergenic (‑46150/‑26400)	unknown ← / → unknown	tRNA‑Ser‑GGA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,105,703	0	C	T	100.0%	150.1 / NA	56	intergenic (‑46150/‑26400)	unknown/unknown	tRNA‑Ser‑GGA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/31);  total (25/31)

GCCTTCAATGGGAATCCAAAGAACGCGGGATAATTTATGGCGGACATGGGAATTTTGCCAGTTCACGCCACTGTTTTGAATAAGTGGCGCGAGACTCACAAAAGTAGTTTCCAGTGGATAACCTTGTTCATTGATGGGCAGGGTTTTCAGCTCAATGCCTAAATGGGTGAAATCCTGTTCCGCTTTACTACCTGCGGTGGCGCCTAACGCCGTTTCAAGCAGCATACCGACCCAGCCTTTATCACGTTTTAAATTCGGTGGCACAGAAATATGCCATTGCGCCGCCAGTTCACCGAAGGTGAGACCGGCAATAGACTGTGCGCGGGAGAGTAATGCCTCGGTGGTTTGCGGGATCATTGGCGATACTTCTTGAAAAAACGCGGTTTAACGATCTCTTTGCCTTTGTCGATATAAGAAATATTTTCCAGTTGCAACAAAAAGCGCTTGGCAACCAAACCGCCACCGAAGCCGGTAAGCGTTCCGT  >  1/1105459‑1105942                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                gCCTTCAATGGGAATCCAAAGAACGCGGGATAATTTATGGCGGACATGGGAATTTTGCCAGTTCACGCCACTGTTTTGAATAAGTGGCGCGAGACTCACAAAAGTAGTTTCCAGTGGATAACCTTGTTCATTGATGGGCAGGGTTTTCAGCTCAATGCCTAAATGGGTGAAATCCTGTTCCGCTTTACTACCTGCGGTGGCGCCTAACGCCGTTTCAAGCAGCATACCGACCCAGCCTTTATCATGTTTTa                                                                                                                                                                                                                                           >  1:480382/1‑251 (MQ=255) gCCTTCAATGGGAATCCAAAGAACGCGGGATAATTTATGGCGGACATGGGAATTTTGCCAGTTCACGCCACTGTTTTGAATAAGGGGCGCGAGACTCACAAAAGTAGTTTCCAGTGGATAACCTTGTTCATTGATGGGCAGGGTTTTCAGCTCAATGCCTAAATGGGGGAAATCCTGTTCCGCTTTACTACCTGCGGTGGCGCCTAACGCCGTTTCAAGCAGCATACCGACCCAGCCTTTATCATGTTTTa                                                                                                                                                                                                                                           >  1:714569/1‑251 (MQ=255)   cTTCAATGGGAATCCAAAGAACGCGGGATAATTTATGGCGGACATGGGAATTTTGCCAGTTCACGCCACTGTTTTGAATAAGTGGCGCGAGACTCACAAAAGTAGTTTCCAGTGGATAACCTTGTTCATTGATGGGCAGGGTTTTCAGCTCAATGCCTAAATGGGTGAAATCCTGTTCCGCTTTACTACCTGCGGTGGCGCCTAACGCCGTTTCAAGCAGCATACCGACCCAGCCTTTATCATGttt                                                                                                                                                                                                                                             <  1:518459/247‑1 (MQ=255)             aaTCCAAAGAACGCGGGATAATTTATGGCGGACATGGGAATTTTGCCAGTTCACGCCACTGTTTTGAATAAGTGGCGCGAGACTCACAAAAGTAGTTTCCAGTGGATAACCTTGTTCATTGATGGGCAGGGTTTTCAGCTCAATGCCTAAATGGGTGAAATCCTGTTCCGCTTTACTACCTGCGGTGGCGCCTAACGCCGTTTCAAGCAGCATACCGACCCAGCCTTTATCATGTTTTAAATTCGGTGGca                                                                                                                                                                                                                               >  1:756337/1‑251 (MQ=255)                   aaGAACGCGGGATAATTTATGGCGGACATGGGAATTTTGCCAGTTCACGCCACTGTTTTGAATAAGTGGCGCGAGACTCACAAAAGTAGTTTCCAGTGGATAACCTTGTTCATTGATGGGCAGGGTTTTCAGCTCAATGCCTAAATGGGTGAAATCCTGTTCCGCTTTACTACCTGCGGTGGCGCCTAACGCCGTTTCAAGCAGCATACCGACCCAGCCTTTATCATGTTTTAAATTCGGTGGCACAGaaa                                                                                                                                                                                                                         <  1:127015/251‑1 (MQ=255)                   aaGAACGCGGGATAATTTATGGCGGACATGGGAATTTTGCCAGTTCACGCCACTGTTTTGAATAAGTGGCGCGAGACTCACAAAAGTAGTTTCCAGTGGATAACCTTGTTCATTGATGGGCAGGGTTTTCAGCTCAATGCCTAAATGGGTGAAATCCTGTTCCGCTTTACTACCTGCGGTGGCGCCTAACGCCGTTTCAAGCAGCATACCGACCCAGCCTTTATCATGTTTTAAATTCGGTGGCACAGaaa                                                                                                                                                                                                                         <  1:224861/251‑1 (MQ=255)                          cgGGATAATTTATGGCGGACATGGGAATTTTGCCAGTTCACGCCACTGTTTTGAATAAGTGGCGCGAGACTCACAAAAGTAGTTTCCAGTGGATAACCTTGTTCATTGATGGGCAGGGTTTTCAGCTCAATGCCTAAATGGGTGAAATCCTGTTCCGCTTTACTACCTGCGGTGGCGCCTAACGCCGTTTCAAGCAGCATACCGACCCAGCCTTTATCATGTTTTAAATTCGGTGGCACAGAAATATGcc                                                                                                                                                                                                                   <  1:304795/250‑1 (MQ=255)                                                  gAATTTTGCCAGTTCACGCCACTGTTTTGAATAAGTGGCGCGAGACTCACAAAAGTAGTTTCCAGTGGATAACCTTGTTCATTGATGGGCAGGGTTTTCAGCTCAATGCCTAAATGGGTGAAATCCTGTTCCGCTTTACTACCTGCGGTGGCGCCTAACGCCGTTTCAAGCAGCATACCGACCCAGCCTTTATCATGTTTTAAATTCGGTGGCACAGAAATATGCCATTGCGCCGCCAGTTCACCGAAgg                                                                                                                                                                                           <  1:559624/250‑1 (MQ=255)                                                       ttGCCAGTTCACGCCACTGTTTTGAATAAGTGGCGCGAGACTCACAAAAGTAGTTTCCAGTGGATAACCTTGTTCATTGATGGGCAGGGTTTTCAGCTCAATGCCTAAATGGGTGAAATCCTGTTCCGCTTTACTACCTGCGGTGGCGCCTAACGCCGTTTCAAGCAGCATACCGACCCAGCCTTTATCATGTTTTAAATTCGGTGGCACAGAAATATGCCATTGCGCCGCCAGTTCACCGAAGGTGAGAc                                                                                                                                                                                     <  1:596430/251‑1 (MQ=255)                                                             gTTCACGCCACTGTTTTGAATAAGTGGCGCGAGACTCACAAAAGTAGTTTCCAGTGGATAACCTTGTTCATTGATGGGCAGGGTTTTCAGCTCAATGCCTAAATGGGTGAAATCCTGTTCCGCTTTACTACCTGCGGTGGCGCCTAACGCCGTTTCAAGCAGCATACCGCCCCAGCCTTTATCATGTTTTAAATTCGGTGGCACAGAAATATGCCATTGCGCCGCCAGTTCACCGAAGGTGAGACCGGCaa                                                                                                                                                                               >  1:439136/1‑251 (MQ=255)                                                                             tGAATAAGTGGCGCGAGACTCACAAAAGTAGTTTCCAGTGGATAACCTTGTTCATTGATGGGCAGGGTTTTCAGCTCAATGCCTAAATGGGTGAAATCCTGTTCCGCTTTACTACCTGCGGTGGCGCCTAACGCCGTTTCAAGCAGCATACCGACCCAGCCTTTATCATGTTTTAAATTCGGTGGCACAGAAATATGCCATTGCGCCGCCAGTTCACCGAAGGTGAGACCGGCAATAGACTGTGCGCGGga                                                                                                                                                               >  1:504888/1‑251 (MQ=255)                                                                               aaTAAGTGGCGCGAGACTCACAAAAGTAGTTTCCAGTGGATAACCTTGTTCATTGATGGGCAGGGTTTTCAGCTCAATGCCTAAATGGGTGAAATCCTGTTCCGCTTTACTACCTGCGGTGGCGCCTAACGCCGTTTCAAGCAGCATACCGACCCAGCCTTTATCATGTTTTAAATTCGGTGGCACAGAAATATGCCATTGCGCCGCCAGTTCACCGAAGGTGAGACCGGCAATAGACTGTGCGCGGgag                                                                                                                                                              <  1:662544/250‑1 (MQ=255)                                                                                  aaGTGGCGCGAGACTCACAAAAGTAGTTTCCAGTGGATAACCTTGTTCATTGATGGGCAGGGTTTTCAGCTCAATGCCTAAATGGGTGAAATCCTGTTCCGCTTTACTACCTGCGGTGGCGCCTAACGCCGTTTCAAGCAGCATACCGACCCAGCCTTTATCATGTTTTAAATTCGGTGGCACAGAAATATGCCATTGCGCCGCCAGTTCACCGAAGGTGAGACCGGCAATAGACTGTGCGCGGGAGAGTa                                                                                                                                                          >  1:94950/1‑251 (MQ=255)                                                                                   aGTGGCGCGAGACTCACAAAAGTAGTTTCCAGTGGATAACCTTGTTCATTGATGGGCAGGGTTTTCAGCTCAATGCCTAAATGGGTGAAATCCTGTTCCGCTTTACTACCTGCGGTGGCGCCTAACGCCGTTTCAAGCAGCATACCGACCCAGCCTTTATCATGTTTTAAATTCGGTGGCACAGAAATATGCCATTGCGCCGCCAGTTCACCGAAGGTGAGACCGGCAATAGAcc                                                                                                                                                                         <  1:160700/235‑2 (MQ=255)                                                                                              aCTCACAAAAGTAGTTTCCAGTGGATAACCTTGTTCATTGATGGGCAGGGTTTTCAGCTCAATGCCTAAATGGGTGAAATCTTGTTCCGCTTTACTACCTGCGGTGGCGCCTAACGCCGTTTCAAGCAGCATACCGACCCAGCCTTTATCATGTTTTAAATTCGGTGGCACAGAAATATGCCATTGCGCCGCCAGTTCACCGAAGGTGAGACCGGCAATAGACTGTGCGCGGGAGAGTAATGCCTCggtgg                                                                                                                                              <  1:425048/251‑1 (MQ=255)                                                                                                 cacaAAAGTAGTTTCCAGTGGATAACCTTGTTCATTGATGGGCAGGGTTTTCAGCTCAATGCCTAAATGGGTGAAATCCTGTTCCGCTTTACTACCTGCGGTGGCGCCTAACGCCGTTTCAAGCAGCATACCGACCCAGCCTTTATCATGTTTTAAATTCGGTGGCACAGaaa                                                                                                                                                                                                                         <  1:109098/173‑1 (MQ=255)                                                                                                 cacaAAAGTAGTTTCCAGTGGATAACCTTGTTCATTGATGGGCAGGGTTTTCAGCTCAATGCCTAAATGGGTGAAATCCTGTTCCGCTTTACTACCTGCGGTGGCGCCTAACGCCGTTTCAAGCAGCATACCGACCCAGCCTTTATCATGTTTTAAATTCGGTGGCACAGAAATATGCCATTg                                                                                                                                                                                                               <  1:831714/183‑1 (MQ=255)                                                                                                 cacaAAAGTAGTTTCCAGTGGATAACCTTGTTCATTGATGGGCAGGGTTTTCAGCTCAATGCCTAAATGGGTGAAATCCTGTTCCGCTTTACTACCTGCGGTGGCGCCTAACGCCGTTTCAAGCAGCATACCGACCCAGCCTTTATCATGTTTTAAATTCGGTGGCACAAAAATATGCCATTGCGCCGCCAGTTCACCGAAGGTGAGACCGGCAATAGACTGTGCGCGGGAGAGTAATGCCTCGGTGGttt                                                                                                                                           >  1:425965/1‑251 (MQ=255)                                                                                                   caAAAGTAGTTTCCAGTGGATAACCTTGTTCATTGATGGGCAGGGTTTTCAGCTCAATGCCTAAATGGGTGAAATCCTGTTCCGCTTTACTACCTGCGGTGGCGCCTAACGCCGTTTCAAGCAGCATACCGACCCAGCCTTTATCATGTTTTAAATTCGGTGGCACAGAAATATGCCATTGCGCCGCCAGTTCACCGAAgg                                                                                                                                                                                           <  1:625014/201‑1 (MQ=255)                                                                                                            tttCCAGTGGATAACCTTGTTCATTGATGGGCAGGGTTTTCAGCTCAATGCCTAAATGGGTGAAATCCTGTTCCGCTTTACTACCTGCGGTGGCGCCTAACGCCGTTTCAAGCAGCATACCGACCCAGCCTTTATCATGTTTTAAATTCGGTGGCACAGAAATATGCCATTGCGCCGCCAGTTCACCGAAGGTGAGACCGGCAATAGACTGTGCGCGGGAGAGTAATGCCTCGGTGGTTTGCGGGATCa                                                                                                                                  <  1:824598/249‑1 (MQ=255)                                                                                                               ccAGTGGATAACCTTGTTCATTGATGGGCAGGGTTTTCAGCTCAATGCCTAAATGGGTGAAATCCTGTTCCGCTTTACTACCTGCGGTGGCGCCTAACGCCGTTTCAAGCAGCATACCGACCCAGCCTTTATCATGTTTTAAATTCGGTGGCACAGAAATATGCCATTGCGCCGCCAGTTCACCGAAGGTGAGACCGGCAATAGACTGTGCGCGGGAGAGTAATGCCTCGGTGGTTTGCGGGATCATTgg                                                                                                                              >  1:78249/1‑250 (MQ=255)                                                                                                                    ggATAACCTTGTTCATTGATGGGCAGGGTTTTCAGCTCAATGCCTAAATGGGTGAAATCCTGTTCCGCTTTACTACCTGCGGTGGCGCCTAACGCCGTTTCAAGCAGCATACCGACCCAGCCTTTATCATGTTTTAAATTCGGTGGCACAGAAATATGCCATTGCGCCGCCAGTTCACCGAAGGTg                                                                                                                                                                                         >  1:142224/1‑186 (MQ=255)                                                                                                                    ggATAACCTTGTTCATTGATGGGCAGGGTTTTCAGCTCAATGCCTAAATGGGTGAAATCCTGTTCCGCTTTACTACCTGCGGTGGCGCCTAACGCCGTTTCAAGCAGCATACCGACCCAGCCTTTATCATGTTTTAAATTCGGTGGCACAGAAATATGCCATTGCGCCGCCAGTTCACCGAAGGTGAGACCGGCAATAGACTGTGCGCGGGAGAGTaa                                                                                                                                                         >  1:866671/1‑218 (MQ=255)                                                                                                                    ggATAACCTTGTTCATTGATGGGCAGGGTTTTCAGCTCAATGCCTAAATGGGTGAAATCCTGTTCCGCTTTACTACCTGCGGTGGCGCCTAACGCCGTTTCAAGCAGCATACCGACCCAGCCTTTATCATGTTTTAAATTCGGTGGCACAGAAATATGCCATTGCGCCGCCAGTTCACCGAAGGTGAGACCGGCAATAGACTGTGCGCGGGAGAGTAATGCCTCGGTGGTTTGCGGGATCATTGGCGATa                                                                                                                         >  1:718799/1‑250 (MQ=255)                                                                                                                              gTTCATTGATGGGCAGGGTTTTCAGCTCAATGCCTAAATGGGTGAAATCCTGTTCCGCTTTACTACCTGCGGTGGCGCCTAACGCCGTTTCAAGCAGCATACCGACCCAGCCTTTATCATGTTTTAAATTCGGTGGCACAGAAATATGCCATTGCGCCGCCAGTTCACCGAAGGTGAGACCGGCAATAGACTGTGCGCggg                                                                                                                                                                <  1:648202/201‑1 (MQ=255)                                                                                                                                     gATGGGCAGGGTTTTCAGCTCAATGCCTAAATGGGTGAAATCCTGTTCCGCTTTACTACCTGCGGTGGCGCCTAACGCCGTTTCAAGCAGCATACCGACCCAGCCTTTATCATGTTTTAAATTCGGTGGCACAGAAATATGCCATTGCGCCGCCAGTTCACCGAAGGTGAGACCGGCAATAGACTGTGCGCGGGAGAGTAATGCCt                                                                                                                                                    >  1:26245/1‑206 (MQ=255)                                                                                                                                             gggTTTTCAGCTCCATGCCTAAATGGGTGAAATCCTGTTCCGCTTTACTACCTGCGGTGGCGCCTAACGCCGTTTCAAGCAGCATACCGACCCAGCCTTTATCATGTTTTAAATTCGGTGGCACAGAAATATGCCATTGCGCCGCCAGTTCACCGAAGGTGAGACCGGCAATAGACTGTGCGCGGGAGAGTAATGCCTCGGTGGTTTGCGGGATCATTGGCGATACTTCTTGAAAAAACGCGGTTTAACGa                                                                                               >  1:157585/1‑251 (MQ=255)                                                                                                                                               gTTTTCAGCTCAATGCCTAAATGGGCGAAATCCTGTTCCGCTTTACTACCTGCGGTGGCGCCTAACGCCGTTTCAAGCAGCATACCGACCCAGCCTTTATCATGTTTTAAATTCGGTGGCACAGAAATATGCCATTGCGCCGCCAGTTCACCGAAGGTGAGACCGGCAATAGACTGTGCGCGGGAGAGTAATGCCTCGGTGGTTTGCGGGATCATTGGCGATACTTCTTGAAAAAACGCGGTTTAACGa                                                                                               >  1:520258/1‑249 (MQ=255)                                                                                                                                                 tttCAGCTCAATGCCTAAATGGGTGAAATCCTGTTCCGCTTTACTACCTGCGGTGGCGCCTAACGCCGTTTCAAGCAGCATACCGACCCAGCCTTTATCATGTTTTAAATTCGGTGGCACAGAAATATGCCATTGCGCCGCCAGTTCACCGAAGGTGAGACCGGCAATAGACTGTGCGCGGGAGAGTAATGcc                                                                                                                                                     <  1:351183/193‑1 (MQ=255)                                                                                                                                                 tttCAGCTCAATGCCTAAATGGGTGAAATCCTGTTCCGCTTTACTACCTGCGGTGGCGCCTAACGCCGTTTCAAGCAGCATACCGACCCAGCCTTTATCATGTTTTAAATTCGGTGGCACAGAAATATGCCATTGCGCCGCCAGTTCACCGAAGGTGAGACCGGCAATAGACTGTGCGCGGGAGAGTAATGCCTCGGTGGTTTGCGGGATCATTGGCGATACTTCTTGAAAAAACGCGGTTTAACGAtctc                                                                                           <  1:723240/251‑1 (MQ=255)                                                                                                                                                    cAGCTCAATGCCTAAATGGGTGAAATCCTGTTCCGCTTTACTACCTGCGGTGGCGCCTAACGCCGTTTCAAGCAGCATACCGACCCAGCCTTTATCATGTTTTAAATTCGGTGGCACAGAAATATGcc                                                                                                                                                                                                                   >  1:858636/1‑128 (MQ=255)                                                                                                                                                      gCTCAATGCCTAAATGGGTGAAATCCTGTTCCGCTTTACTACCTGCGGTGGCGCCTAACGCCGTTTCAAGCAGCATACCGACCCAGCCTTTATCATGTTTTAAATTCGGTGGCACAGAAATATGCCATTGCGCCGCCAGTTCACCGAAGGTGAGACCGGCAATAGACTGTGCGCGGGAGAGTAATGCCt                                                                                                                                                    <  1:135552/189‑1 (MQ=255)                                                                                                                                                       cTCAATGCCTAAATGGGTGAAATCCTGTTCCGCTTTACTACCTGCGGTGGCGCCTAACGCCGTTTCAAGCAGCATACCGACCCAGCCTTTATCATGTTTTAAATTCGGTGGCACAGAAATATGCCATTGCGCCGCCAGTTCACCGAAGGTGAGACCGGCAATAGACTGTGCGCGGGAGAGTAATGCCTCGGTGGTTTGCGGGATCATTGGCGATACTTCTTGAAAAAACGCGGTTTAACGATCTCTTTGcc                                                                                     <  1:507285/251‑1 (MQ=255)                                                                                                                                                             gCCTAAATGGGTGAAATCCTGTTCCGCTTTACTACCTGCGGTGGCGCCTAACGCCGTTTCAAGCAGCATACCGACCCAGCCTTTATCATGTTTTAAATTCGGTGGCACAGAAATATGCCATTGCGCCGCCAGTTCACCGAAGGTGAGACCGGCAATAg                                                                                                                                                                            <  1:91199/158‑1 (MQ=255)                                                                                                                                                             gCCTAAATGGGTGAAATCCTGTTCCGCTTTACTACCTGCGGTGGCGCCTAACGCCGTTTCAAGCAGCATACCGACCCAGCCTTTATCATGTTTTAAATTCGGTGGCACAGAAATATGCCATTGCGCCGCCAGTTCACCGAAGGTGAGACCGGCAATAGACTGTGCGCGGGAGAGTAATGCCt                                                                                                                                                    <  1:649920/182‑1 (MQ=255)                                                                                                                                                             gCCTAAATGGGTGAAATCCTGTTCCGCTTTACTACCTGCGGTGGCGCCTAACGCCGTTTCAAGCAGCATACCGACCCAGCCTTTATCATGTTTTAAATTCGGTGGCACAGAAATATGCCATTGCGCCGCCAGTTCACCGAAGGTGAGACCGGCAATAGACTGTGCGCGGAAGAGTAATGCCTCGGTGGTTTGCGGGATCATTGGCGATACTTCTTGAAAAAACGCGGTTTAACGATCTCTTTGCCTTTGt                                                                                >  1:429497/1‑250 (MQ=255)                                                                                                                                                               cTAAATGGGTGAAATCCTGTTCCGCTTTACTACCTGCGGTGGCGCCTAACGCCGTTTCAAGCAGCATACCGACCCAGCCTTTATCATGTTTTAAATTCGGTGGCACAGAAATATGCCATTGCGCCGCCAGTTCACCGAAGGTGAGACCGGCAATAGACTGTGCGCGGGAGAGTAATGCCTCGGTGGTTTGCGGGATCATTGGCGATACTTCTTGAAAAAACGCGGTTTAACGATCTCTTTGCCTTTGTCGa                                                                             >  1:502322/1‑251 (MQ=255)                                                                                                                                                                      ggTGAAATCCTGTTCCGCTTTACTACCTGCGGTGGCGCCTAACGCCGTTTCAAGCAGCATACCGACCCAGCCTTTATCATGTTTTAAATTCGGTGGCACAGAAATATGCCATTGCGCCGCCAGTTCACCGAAGGTGAGACCg                                                                                                                                                                                   <  1:523612/142‑1 (MQ=255)                                                                                                                                                                      ggTGAAATCCTGTTCCGCTTTACTACCTGCGGTGGCGCCTAACGCCGTTTCAAGCAGCATACCGACCCAGCCTTTATCATGTTTTAAATTCGGTGGCACAGAAATATGCCATTGCGCCGCCAGTTCACCGAAGGTGAGACCGGCAATAGACTGTGCGCGGGAGAGTAATGCCTCGGTGGTTTGCGGGATCATTGGCGATACTTCTTGAAAAAACGCGGTTTAACGATCTCTTTGCCTTTGTCGATATAAGa                                                                      >  1:213510/1‑251 (MQ=255)                                                                                                                                                                           aaTCCTGTTCCGCTTTACTACCTGCGGTGGCGCCTAACGCCGTTTCAAGCAGCATACCGACCCAGCCTTTATCATGTTTTAAATTCGGTGGCACAGAAATATGCCATTGCGCCGCCAGTTCACCGAAGGTGAGACCGGCAATAGACTGTGCGCGGGAGAGTAATGCCTCGGTGGTTTGCGGGATCATTGGCGATACTTCTTGAAAAAACGCGGTTTAACGATCTCTTTGCCTTTGTCGATATAAGAAatat                                                                 <  1:616363/251‑1 (MQ=255)                                                                                                                                                                            aTCCTGTTCCGCTTTACTACCTGCGGTGGCGCCTAACGCCGTTTCAAGCAGCATACCGACCCAGCCTTTATCATGTTTTAAATTCGGTGGCACAGAAATATGCCATTGCGCCGCCAGTTCACCGAAGGTGAGACCGGCAATAGACTGTGCGCGGGAGAGTAATGCCTCGGTGGTTTGCGGGATCATTGGCGATACTTCTTGAAAAAACGCGGTTTAACGATCTCTTTGCCt                                                                                    <  1:303467/231‑1 (MQ=255)                                                                                                                                                                            aTCCTGTTCCGCTTTACTACCTGCGGTGGCGCCTAACGCCGTTTCAAGCAGCATACCGACCCAGCCTTTATCATGTTTTAAATTCGGTGGCACAGAAATATGCCATTGCGCCGCCAGTTCACCGAAGGTGAGACCGGCAATAGACTGTGCGCGGGAGAGTAATGCCTCGGTGGTTTGCGGGATCATTGGCGATACTTCTTGAAAAAACGCGGTTTAACGATCTCTTTGCCTTTGTCGATATAAGAAATAtt                                                                >  1:531079/1‑251 (MQ=255)                                                                                                                                                                                             tacCTGCGGTGGCGCCTAACGCCGTTTCAAGCAGCATACCGACCCAGCCTTTATCATGTTTTAAATTCGGTGGCACAGAAATATGCCATTGCGCCGCCAGTTCACCGAAGGTGAGACCGGCAATAGACTGTGCGCGGGAGAGTAATGCCTCGGTGGTTTGCGGGATCATTGGCGATACTTCTTGAAAAAACGCGGTTTAACGATCTCTTTGCCTTTGTCGATATAAGAAATATTTTCCAGTTGCAACaaaa                                               >  1:860898/1‑251 (MQ=255)                                                                                                                                                                                                         cgcCTAACGCCGTTTCAAGCAGCATACCGACCCAGCCTTTATCATGTTTTAAATTCGGTGGCACAGAAATATGCCATTGCGCCGCCAGTTCACCGAAGGTGAGACCGGCAATAGACTGTGCGCGGGAGAGTAATGCCTCGGTGGTTTGCGGGATCATTGGCGATACTTCTTGAAAAAACGCGGTTTAACGATCTCTTTGCCTTTGTCGATATAAGAAATATTTTCCAg                                                          <  1:17091/228‑1 (MQ=255)                                                                                                                                                                                                          gcCTAACGCCGTTTCAAGCAGCATACCGACCCAGCCTTTATCATGTTTTAAATTCGGTGGCACAGAAATATGCCATTGCGCCGCCAGTTCACCGAAGGTGAGACCGGCAATAGAc                                                                                                                                                                          >  1:602454/1‑115 (MQ=255)                                                                                                                                                                                                                      ttCAAGCAGCATACCGACCCAGCCTTTATCATGTTTTAAATTCGGTGGCACAGAAATATGCCATTGCGCCGCCAGTTCACCGAAGGTGAGACCGGCAATAGAc                                                                                                                                                                          <  1:300099/103‑1 (MQ=255)                                                                                                                                                                                                                            cagcaTACCGACCCAGCCTTTATCATGTTTTAAATTCGGTGGCACAGAAATATGCCATTGCGCCGCCAGTTCACCGAAGGTGAGACCGGCAATAGACTGTGCGCGGGAGAGTAATGCCTCGGTGGTTTGCGGGATCATTGGCGATACTTCTTGAAAAAACGCGGTTTAACGATCTCTTTGCCTTTGTCGATATAAGAAATATTTTCCAGTTGCAACAAAAAGCGCTTGGCAACCAAACCGCCACCGAAg                  <  1:181205/249‑1 (MQ=255)                                                                                                                                                                                                                              gcaTACCGACCCAGCCTTTATCATGTTTTAAATTCGGTGGCACAGAAATATGCCATTGCGCCGCCAGTTCACCGAAGGTGAGACCGGCAATAGACTGTGCGCGGGAGAGTAATGCCTCGGTGGTTTGCGGGATCATTGGCGATACTTCTTGAAAAAACGCGGTTTAACGATCTCTTTGCCTTTGTCGATATAAGAAATATTTTCCAGTTGcaa                                                    >  1:676234/1‑213 (MQ=255)                                                                                                                                                                                                                              gcaTACCGACCCAGCCTTTATCATGTTTTAAATTCGGCGGCACAGAAATATGCCATTGCGCCGCCAGTTCACCGAAGGTGAGACCGGCAATAGACTGTGCGCGGGAGAGTAATGCCTCGGTGGTTTGCGGGATCATTGGCGATACTTCTTGAAAAAACGCGGTTTAACGATCTCTTTGCCTTTGTCGATATAAGAAATATTTTCCAGTTGCAACAAAAAGCGCTTGGCAACCAAACCGCCACCGAAGCCgg              >  1:414968/1‑251 (MQ=255)                                                                                                                                                                                                                                   ccGACCCAGCCTTTATCATGTTTTAAATTCGGTGGCACAGAAATATGCCATTGCGCCGCCAGTTCACCGAAGGTGAGACCGGCAATAGACTGTGCGCGGGAGAGTAATGCCTCGGTGGTTTGCGGGATCATTGGCGATACTTCTTGAAAAAACGCGGTTTAACGATCTCTTTGCCTTTGTCGATATAAGAAATATTTTCCAGTTGCAACAAAAAGCGCTTGGCAACCAAACCGCCACCGAAGCCGGTAAGc         <  1:482060/251‑1 (MQ=255)                                                                                                                                                                                                                                   ccGACCCAGCCTTTATCATGTTTTAAATTCGGTGGCACAGAAATATGCCATTGCGCCGCCAGTTCACCGAAGGTGAGACCGGCAATAGACTGTGCGCGGGAGAGTAATGCCTCGGTGGTTTGCGGGATCATTGGCGATACTTCTTGAAAAAACGCGGTTTAACGATCTCTTTGCCTTTGTCGATATAAGAAATATTTTCCAGTTGCAACAAAAAGCGCTTGGCAACCAAACCGCCACCGAAGCCGGTAAGc         <  1:797438/251‑1 (MQ=255)                                                                                                                                                                                                                                   ccGACCCAGCCTTTATCATGTTTTAAATTCGGTGGCACAGAAATATGCCATTGCGCCGCCAGTTCACCGAAGGTGAGACCGGCAATAGACTGTGCGCGGGAGAGTAATGCCTCGGTGGTTTGCGGGATCATTGGCGATACTTCTTGAAAAAACGCGGTTTAACGATCTCTTTGCCTTTGTCGATATAAGAAATATTTTCCAGTTGCAACAAAAAGCGCTTGGCAACCAAACCGCCACCGAAGCCGGTAAGc         <  1:541188/251‑1 (MQ=255)                                                                                                                                                                                                                                     gACCCAGCCTTTATCATGTTTTAAATTCGGTGGCACAGAAATATGCCATTGCACCGCCAGTTCACCGAAGGTg                                                                                                                                                                                         <  1:527836/73‑1 (MQ=255)                                                                                                                                                                                                                                      aCCCAGCCTTTATCATGTTTTAAATTCGGTGGCACAGAAATATGCCATTGCGCCGCCAGTTCACCGAAGGTGAGACCGGCAATAGACTGTGCGCGGGAGAGTAATGCCTCGGTGGTTTGCGGGATCATTGGCGATACTTCTTGAAAAAACGCGGTTTAACGATCTCTTTACCTTTGTCGATATAAGAAATATTTTCCAGTTGCAACAAAAAGCGCTTGGCAACCAAACCGCCACCGAAGCCGGTAAGCGtt      >  1:454238/1‑251 (MQ=255)                                                                                                                                                                                                                                           gCCTTTATCATGTTTTAAATTCGGTGGCACAGAAATATGCCATTGCGCCGCCAGTTCACCGAAGGTGAGACCGGCAATAGACTGTGCGCGGGAGAGTAATGCCTCGGTGGTTTGCGGGATCATTGGCGATACTTCTTGAAAAAACGCGGTTTAACGa                                                                                               <  1:658306/157‑1 (MQ=255)                                                                                                                                                                                                                                            ccTTTATCATGTTTTAAATTCGGTGGCACAGAAATATGCCATTGCGCCGCCAGTTCACCGAAGGTGAGACCGGCAATAGACTGTGCGCGGGAGAGTAATGCCTCGGTGGTTTGCGGGATCATTGGCGATACTTCTTGAAAAAACGCGGTTTAACGATCTCTTTGCCTTTGTCGATATAAGAAATATTTTCCAGTTGCAACAAAAAGCGCTTGGCAACCAAACCGCCACCGAAGCCGGTAAGCGTTCCGt  >  1:643851/1‑249 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                GCCTTCAATGGGAATCCAAAGAACGCGGGATAATTTATGGCGGACATGGGAATTTTGCCAGTTCACGCCACTGTTTTGAATAAGTGGCGCGAGACTCACAAAAGTAGTTTCCAGTGGATAACCTTGTTCATTGATGGGCAGGGTTTTCAGCTCAATGCCTAAATGGGTGAAATCCTGTTCCGCTTTACTACCTGCGGTGGCGCCTAACGCCGTTTCAAGCAGCATACCGACCCAGCCTTTATCACGTTTTAAATTCGGTGGCACAGAAATATGCCATTGCGCCGCCAGTTCACCGAAGGTGAGACCGGCAATAGACTGTGCGCGGGAGAGTAATGCCTCGGTGGTTTGCGGGATCATTGGCGATACTTCTTGAAAAAACGCGGTTTAACGATCTCTTTGCCTTTGTCGATATAAGAAATATTTTCCAGTTGCAACAAAAAGCGCTTGGCAACCAAACCGCCACCGAAGCCGGTAAGCGTTCCGT  >  1/1105459‑1105942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑