Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,104,233	G→A	intergenic (‑44680/‑27870)	unknown ← / → unknown	tRNA‑Ser‑GGA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,104,233	0	G	A	100.0%	75.6 / NA	31	intergenic (‑44680/‑27870)	unknown/unknown	tRNA‑Ser‑GGA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/17);  total (14/17)

TTTTAGGGCGTTGAGGGTTTATATAAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATTCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGTTTACGCATTTGGGTATTGAGAATTTCCACAAATACGGAAAAGCCCATAGCGGAGTAAATATACGCTTTCGGCACATGGATGCTGAAGCTCTCGATAA  >  1/1104080‑1104475                                                                                                                                                          |                                                                                                                                                                                                                                                   ttttAGGGCGTTGAGGGTTTATATAAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAAtg                                                                                                                                                   <  1:46503/251‑1 (MQ=255)   ttAGGGCGTTGAGGGTTTATATAAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAAtgtg                                                                                                                                                 <  1:150356/251‑1 (MQ=255)               gggTTTATATAAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCg                                                                                                                                      >  1:342099/1‑250 (MQ=255)                     atataAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACtttt                                                                                                                               >  1:657698/1‑251 (MQ=255)                      tataAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTg                                                                                                                              >  1:535620/1‑251 (MQ=255)                         aaGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGttt                                                                                                                           <  1:566179/251‑1 (MQ=255)                         aaGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGttt                                                                                                                           <  1:249735/251‑1 (MQ=255)                          aGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGttt                                                                                                                           >  1:465716/1‑250 (MQ=255)                                       ccATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGTTTCTCTAAATTTTAg                                                                                                              >  1:292611/1‑250 (MQ=255)                                                 ctctTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGt                                                                                                   >  1:121836/1‑251 (MQ=255)                                                   ctTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGTTTCTCTAAATTTTAGCGCCCAAGCg                                                                                                    <  1:212050/248‑1 (MQ=255)                                                     ttCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGTTTAc                                                                                               <  1:286532/251‑1 (MQ=255)                                                      tCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCTCTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGTTTACg                                                                                              <  1:471639/251‑1 (MQ=17)                                                                cTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGTTTACGCATTTGGGt                                                                                     <  1:518045/250‑1 (MQ=255)                                                                 ttttCCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCGCTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGTTTACGCATTTGGGTAt                                                                                   <  1:429464/251‑1 (MQ=18)                                                                 ttttCCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGTTTACGCATTTGGGTAt                                                                                   <  1:248147/251‑1 (MQ=255)                                                                     ccAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGTTTACGCATTTGGGTATTgag                                                                               <  1:157598/251‑1 (MQ=255)                                                                      cAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGTTTACGCATTTGGGTATTgaga                                                                              <  1:247082/251‑1 (MQ=255)                                                                                         gagaAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGt                                                                                                                             >  1:764170/1‑185 (MQ=14)                                                                                            aaaTCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGTTTACGCATTTGGGTATTGAGAATTTCCACAAATACGGAAAAg                                                         <  1:75044/250‑1 (MQ=255)                                                                                                   atgaAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGTTTACGCATTTGGGTATTGAGAATTTCCACAAATACGGAAAAGCCCATAGc                                                 <  1:470504/251‑1 (MQ=255)                                                                                                   atgaAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGTTTACGCATTTGGGTATTGAGAATTTCCACAAATACGGAAAAGCCCATAGc                                                 <  1:197995/251‑1 (MQ=255)                                                                                                        gttcaGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGTTTACGCATTTGGGTATTGAGAATTTCCACAAATACGGAAAAGCCCATAGCGGAGt                                            >  1:51218/5‑251 (MQ=255)                                                                                                              ccACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGTTTACGCATTTGGGTATTGAGAATTTCCACAAATACGGAAAAGCCCATAGCGGAGTAAatat                                      <  1:519324/251‑1 (MQ=255)                                                                                                                aCCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTG‑TTTTCGACCGCACTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGTTTACGCATTTGGGTATTGAGAATTTCCACAAATACGGAAAAGCCCATAGCGGAGTAAATATACg                                   >  1:245913/1‑251 (MQ=255)                                                                                                                            ccAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGTTTACGCATTTGGGTATTGAGAATTTCCACAAATACGGAAAAGCCCATAGCGGAGTAAATATACGCTTTCGGcaca                        >  1:895989/1‑251 (MQ=255)                                                                                                                                aatccTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGTTTACGCATTTGGGTATTGAGAATTTCCACAAATACGGAAAAGCCCATAGCGGAGTAAATATACGCTTTCGGCACATGGa                    <  1:585994/247‑1 (MQ=255)                                                                                                                                               ccTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGTTTACGCATTTGGGTATTGAGAATTTCCACAAATACGGAAAAGCCCATAGCGGAGTAAATATACGCTTTCGGCACATGGATGCTGAAGCTCTCGa     >  1:54277/1‑251 (MQ=255)                                                                                                                                                  gTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGAc                                                                                                                                        >  1:810272/1‑117 (MQ=17)                                                                                                                                                  gTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGTTTACGCATTTGGGTATTGAGAATTTCCACAAATACGGAAAAGCCCATAGCGGAGTaa                                          >  1:581617/1‑211 (MQ=255)                                                                                                                                                  gTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGTTTACGCATTTGGGTATTGAGAATTTCCACAAATACGGAAAAGCCCATAGCGGAGTAAATATACGCTTTCGGCACATGGATGCTGAAGCTCTCGATaa  >  1:14030/1‑251 (MQ=255)                                                                                                                                                          |                                                                                                                                                                                                                                                   TTTTAGGGCGTTGAGGGTTTATATAAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATTCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGTTTACGCATTTGGGTATTGAGAATTTCCACAAATACGGAAAAGCCCATAGCGGAGTAAATATACGCTTTCGGCACATGGATGCTGAAGCTCTCGATAA  >  1/1104080‑1104475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑