Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 1,104,210 T→C intergenic (‑44657/‑27893) unknown ← / → unknown tRNA‑Ser‑GGA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,104,2100TC100.0% 68.6 / NA 26intergenic (‑44657/‑27893)unknown/unknowntRNA‑Ser‑GGA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/16);  total (10/16)

TTTTAGGGCGTTGAGGGTTTATATAAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATTCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGTTTACGCATTTGGGTATTGAGAATTTCCACAAATACGGAAAAGCCCATAGCGGAGTAAATATACGCTTTCGGCACA  >  1/1104080‑1104453
                                                                                                                                  |                                                                                                                                                                                                                                                   
ttttAGGGCGTTGAGGGTTTATATAAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAAtg                                                                                                                             <  1:46503/251‑1 (MQ=255)
  ttAGGGCGTTGAGGGTTTATATAAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAAtgtg                                                                                                                           <  1:150356/251‑1 (MQ=255)
              gggTTTATATAAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCg                                                                                                                >  1:342099/1‑250 (MQ=255)
                    atataAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACtttt                                                                                                         >  1:657698/1‑251 (MQ=255)
                     tataAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTg                                                                                                        >  1:535620/1‑251 (MQ=255)
                        aaGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGttt                                                                                                     <  1:249735/251‑1 (MQ=255)
                        aaGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGttt                                                                                                     <  1:566179/251‑1 (MQ=255)
                         aGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGttt                                                                                                     >  1:465716/1‑250 (MQ=255)
                                      ccATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGTTTCTCTAAATTTTAg                                                                                        >  1:292611/1‑250 (MQ=255)
                                                ctctTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGt                                                                             >  1:121836/1‑251 (MQ=255)
                                                  ctTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGTTTCTCTAAATTTTAGCGCCCAAGCg                                                                              <  1:212050/248‑1 (MQ=255)
                                                    ttCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGTTTAc                                                                         <  1:286532/251‑1 (MQ=255)
                                                     tCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCTCTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGTTTACg                                                                        <  1:471639/251‑1 (MQ=17)
                                                               cTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGTTTACGCATTTGGGt                                                               <  1:518045/250‑1 (MQ=255)
                                                                ttttCCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCGCTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGTTTACGCATTTGGGTAt                                                             <  1:429464/251‑1 (MQ=18)
                                                                ttttCCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGTTTACGCATTTGGGTAt                                                             <  1:248147/251‑1 (MQ=255)
                                                                    ccAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGTTTACGCATTTGGGTATTgag                                                         <  1:157598/251‑1 (MQ=255)
                                                                     cAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGTTTACGCATTTGGGTATTgaga                                                        <  1:247082/251‑1 (MQ=255)
                                                                                        gagaAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGt                                                                                                       >  1:764170/1‑185 (MQ=14)
                                                                                           aaaTCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGTTTACGCATTTGGGTATTGAGAATTTCCACAAATACGGAAAAg                                   <  1:75044/250‑1 (MQ=255)
                                                                                                  atgaAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGTTTACGCATTTGGGTATTGAGAATTTCCACAAATACGGAAAAGCCCATAGc                           <  1:197995/251‑1 (MQ=255)
                                                                                                  atgaAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGTTTACGCATTTGGGTATTGAGAATTTCCACAAATACGGAAAAGCCCATAGc                           <  1:470504/251‑1 (MQ=255)
                                                                                                       gttcaGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGTTTACGCATTTGGGTATTGAGAATTTCCACAAATACGGAAAAGCCCATAGCGGAGt                      >  1:51218/5‑251 (MQ=255)
                                                                                                             ccACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGTTTACGCATTTGGGTATTGAGAATTTCCACAAATACGGAAAAGCCCATAGCGGAGTAAatat                <  1:519324/251‑1 (MQ=255)
                                                                                                               aCCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTG‑TTTTCGACCGCACTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGTTTACGCATTTGGGTATTGAGAATTTCCACAAATACGGAAAAGCCCATAGCGGAGTAAATATACg             >  1:245913/1‑251 (MQ=255)
                                                                                                                           ccAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGTTTACGCATTTGGGTATTGAGAATTTCCACAAATACGGAAAAGCCCATAGCGGAGTAAATATACGCTTTCGGcaca  >  1:895989/1‑251 (MQ=255)
                                                                                                                                  |                                                                                                                                                                                                                                                   
TTTTAGGGCGTTGAGGGTTTATATAAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATTCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGTTTACGCATTTGGGTATTGAGAATTTCCACAAATACGGAAAAGCCCATAGCGGAGTAAATATACGCTTTCGGCACA  >  1/1104080‑1104453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑