Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 1,104,186 T→C intergenic (‑44633/‑27917) unknown ← / → unknown tRNA‑Ser‑GGA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,104,1860TC100.0% 54.4 / NA 22intergenic (‑44633/‑27917)unknown/unknowntRNA‑Ser‑GGA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/15);  total (7/15)

TTTTAGGGCGTTGAGGGTTTATATAAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATTCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGTTTACGCATTTGGGTATTGAGAATTTCCACAAATACGGAAAAGCCCATAGC  >  1/1104080‑1104428
                                                                                                          |                                                                                                                                                                                                                                                  
ttttAGGGCGTTGAGGGTTTATATAAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAAtg                                                                                                    <  1:46503/251‑1 (MQ=255)
  ttAGGGCGTTGAGGGTTTATATAAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAAtgtg                                                                                                  <  1:150356/251‑1 (MQ=255)
              gggTTTATATAAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCg                                                                                       >  1:342099/1‑250 (MQ=255)
                    atataAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACtttt                                                                                >  1:657698/1‑251 (MQ=255)
                     tataAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTg                                                                               >  1:535620/1‑251 (MQ=255)
                        aaGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGttt                                                                            <  1:566179/251‑1 (MQ=255)
                        aaGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGttt                                                                            <  1:249735/251‑1 (MQ=255)
                         aGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGttt                                                                            >  1:465716/1‑250 (MQ=255)
                                      ccATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGTTTCTCTAAATTTTAg                                                               >  1:292611/1‑250 (MQ=255)
                                                ctctTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGt                                                    >  1:121836/1‑251 (MQ=255)
                                                  ctTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGTTTCTCTAAATTTTAGCGCCCAAGCg                                                     <  1:212050/248‑1 (MQ=255)
                                                    ttCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGTTTAc                                                <  1:286532/251‑1 (MQ=255)
                                                     tCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCTCTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGTTTACg                                               <  1:471639/251‑1 (MQ=17)
                                                               cTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGTTTACGCATTTGGGt                                      <  1:518045/250‑1 (MQ=255)
                                                                ttttCCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCGCTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGTTTACGCATTTGGGTAt                                    <  1:429464/251‑1 (MQ=18)
                                                                ttttCCAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGTTTACGCATTTGGGTAt                                    <  1:248147/251‑1 (MQ=255)
                                                                    ccAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGTTTACGCATTTGGGTATTgag                                <  1:157598/251‑1 (MQ=255)
                                                                     cAATAAATGACTTGAGAACGAGAAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGTTTACGCATTTGGGTATTgaga                               <  1:247082/251‑1 (MQ=255)
                                                                                        gagaAATCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGt                                                                              >  1:764170/1‑185 (MQ=14)
                                                                                           aaaTCTGATGAAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGTTTACGCATTTGGGTATTGAGAATTTCCACAAATACGGAAAAg          <  1:75044/250‑1 (MQ=255)
                                                                                                  atgaAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGTTTACGCATTTGGGTATTGAGAATTTCCACAAATACGGAAAAGCCCATAGc  <  1:470504/251‑1 (MQ=255)
                                                                                                  atgaAGTTCAGCCACCTCACGGATACCAAAATCCTGCTCGGTGACCTGTTTGATAATTTCTAAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGTTTACGCATTTGGGTATTGAGAATTTCCACAAATACGGAAAAGCCCATAGc  <  1:197995/251‑1 (MQ=255)
                                                                                                          |                                                                                                                                                                                                                                                  
TTTTAGGGCGTTGAGGGTTTATATAAGGGGATTTTACGCCATTAAGCCCTCTTTCACGAAAGGCTTTTCCAATAAATGACTTGAGAACGAGAAATCTGATGAAATTTAGCCACCTCACGGATACCAAAATTCTGCTCGGTGACCTGTTTGATAGTTTTTTAACGAAATTGATAAGAGTAAGACATAATCTGCACCTCAAATTAGGTGTCCAGGTTTTGGGGTGCAGAGCAAAGTGCGGTTGAAATTTAATGTGTTTTTCGACCGCACTTTTGTTTCTCTAAATTTTAGCGCCCAAGCGTTTACGCATTTGGGTATTGAGAATTTCCACAAATACGGAAAAGCCCATAGC  >  1/1104080‑1104428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑