Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,101,438	T→G	intergenic (‑41885/‑30665)	unknown ← / → unknown	tRNA‑Ser‑GGA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,101,438	0	T	G	100.0%	14.7 / NA	7	intergenic (‑41885/‑30665)	unknown/unknown	tRNA‑Ser‑GGA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (2/5);  total (2/5)

ACAACGTTTTAACATGATCAGCTATTAAATAATGGTGGAAAATTTGAACGAAATCAGAAGAAAAGAGACAAAAATGGTGATCGTGATCACATTAAAGATTCATGAAATTAAATAACGCGTATATAAAGAATAAGGTTGTTTTTGATTTTTTATATTGTTAAATTACAATTTTATTGTATTTTATCTAAGTATTTATTTTTATCATTAATTTTTGTTTAAAAATAACTTGAATTTTATGAATAGTATGCTATTTTTGTAGCGTTTCTTTTTCATTTTACTCATGTCTATTATAGAGGATTTACTTATGCCCAGCGCGATTGAGAATGTATTTAAACTCATTGAAGAAAATGACATTAAATTTGTATTACTGCGTTTTACCGATATTCAAGGC  >  1/1101280‑1101670                                                                                                                                                               |                                                                                                                                                                                                                                         aCAACGTTTTAACATGATCAGCTATTAAATAATGGTGGAAAATTTGAACGAAATCAGAAGAAAAGAGACAAAAATGGTGATCGTGATCACATTAAAGATTCATGAAATTAAATAACGCGTATATAAAGAATAAGGTTGTTTTTGATTTTTTATATTGTGAAATTACAATTTTATTGTATTTTATCTAAGTATTTATTTTTATCATTAATTTTTGTTTAAAAATAACTTGAATTTTATGAATAGTATGCTAt                                                                                                                                              <  1:824784/251‑1 (MQ=255)                        aTTAAATAATGGTGGAAAATTTGAACGAAATCAGAAGAAAAGAGACAAAAATGGTGATCGTGATCACATTAAAGATTCATGAAATTAAATAACGCGTATATAAAGAATAAGGTTGTTTTTGATTTTTTATATTGTGAAATTACAATTTTATTGTATTTTATCTAAGta                                                                                                                                                                                                          <  1:627682/168‑1 (MQ=255)                                         aaTTTGAACGAAATCAGAAGAAAAGAGACAAAAATGGTGATCGTGATCACATTAAAGATTCATGAAATTAAATAACGCGTATATAAAGAATAAGGTTGTTTTTGATTTTTTATATTGTGAAATTACAATTTTATTGTATTTTATCTAAGTATTTATTTTTATCATTAATTTTTGTTTAAAAATAACTTGAATTTTATGAATAGTATGCTATTTTTGTAGCGTTTCTTTTTCATTTTACTCATGTCtattat                                                                                                      >  1:561666/1‑251 (MQ=255)                                                                              tgatcgtgatcACATTAAAGATTCATGAAATTAAATAACGCGTATATAAAGAATAAGGTTGTTTTTGATTTTTTATATTGTGAAATTACAATTTTATTGTATTTTATCTAAGTATTTATTTTTATCATTAATTTTTGTTTAAAAATAACTTGAATTTTATGAATAGTATGCTATTTTTGTAGCGTTTCTTTTTCATTTTACTCATGTCTATTATAGAGGATTTACTTATGCCCAGCGCGATTGAGAATGTa                                                                 <  1:365163/251‑1 (MQ=255)                                                                                                                    cgcgTATATAAAGAATAAGGTTGTTTTTGATTTTTTATATTGTGAAATTACAATTTTATTGTATTTTATCTAAGTATTTATTTTTATCATTAATTTTTGTTTAAAAATAACTTGAATTTTATGAATAGTATGCTATTTTTGTAGCGTTTCTTTTTCATTTTACTCATGTCTATTATAGAGGATTTACTTATGCCCAGCGCGATTGAGAATGTATTTAAACTCATTGAAGAAAATGACATTAAATTTGTAtt                           >  1:634009/1‑251 (MQ=255)                                                                                                                                             tttGATTTTTTATATTGTGAAATTACAATTTTATTGTATTTTATCTAAGTATTTATTTTTATCATTAATTTTTGTTTAAAAATAACTTGAATTTTATGAATAGTATGCTATTTTTGTAGCGTTTCTTTTTCATTTTACTCATGTCTATTATAGAGGATTTACTTATGCCCAGCGCGATTGAGAATGTATTTAAACTCATTGAAGAAAATGACATTAAATTTGTATTACTGCGTTTTACCGATATTCAAGGc  <  1:161041/251‑1 (MQ=255)                                                                                                                                                        atatTGTGAAATTACAATTTTATTGTATTTTATCTAAGTATTTATTTTTATCATTAATTTTTGTTTAAAAATAACTTGAATTTTATGAATAGTATGCTAtttt                                                                                                                                           <  1:457891/103‑1 (MQ=255)                                                                                                                                                               |                                                                                                                                                                                                                                         ACAACGTTTTAACATGATCAGCTATTAAATAATGGTGGAAAATTTGAACGAAATCAGAAGAAAAGAGACAAAAATGGTGATCGTGATCACATTAAAGATTCATGAAATTAAATAACGCGTATATAAAGAATAAGGTTGTTTTTGATTTTTTATATTGTTAAATTACAATTTTATTGTATTTTATCTAAGTATTTATTTTTATCATTAATTTTTGTTTAAAAATAACTTGAATTTTATGAATAGTATGCTATTTTTGTAGCGTTTCTTTTTCATTTTACTCATGTCTATTATAGAGGATTTACTTATGCCCAGCGCGATTGAGAATGTATTTAAACTCATTGAAGAAAATGACATTAAATTTGTATTACTGCGTTTTACCGATATTCAAGGC  >  1/1101280‑1101670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑