Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,080,433	T→C	intergenic (‑20880/‑51670)	unknown ← / → unknown	tRNA‑Ser‑GGA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,080,433	0	T	C	100.0%	171.6 / NA	62	intergenic (‑20880/‑51670)	unknown/unknown	tRNA‑Ser‑GGA/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/37);  total (25/37)

TCAACAAATTGTCCGAGTTGGCGAAGCTGCTTTCATAGGCTTTCAGGCTACGAATGAAGCTGTAAAATTCAGGCTCTTTACCAAAGGCATCGGCAAAGATTTTGGCTGCGGTCGCGTCGCCGTCACCGCGTAATTCCTGTGCGGTTTTTTCCGCATTGGCGATAATTAAGGTGACTTTACGATCTACATCCGCCTGAATAAATGCCGCTTTTTCTTTACCTTGAGAGCGGTGTTCACGGGCTACTGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATCCGGCAAGTTAATTTGTTTAATCCGTACGTCGAGCACTTCAATCCCCAGTTCGGCGGTGCTGTCTTGCCCACTGTTGAGCGCTTTTTTCGTGCCTACCATGAGTTCGCCTCGTGTACCGGAAACGATGTCTTTAA‑TGGTGCGGGAACCGATTTCGGAACGCAAACGGTCATTGACTTTACGACGTAATAAGTTGGC  >  1/1080189‑1080678                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       tCAACAAATTGTCCGAGTCGGCGAAGCTGCTTTCATAGGCTTTCAGGCTACGAATGAAGCTGTAAAATTCAGGCTCTTTACCAAAGGCATCGGCAAAGATTTTGGCTGCGGTCGCGTCGCCGTCACCGCGTAATTCCTGTGCGGTTTTTTCCGCATTGGCGATAATTAAGGTGACTTTACGATCTACATCCGCCTGAATAAATGCCGCTTTTTCTTTACCTTGAGAGCGGTGTTCACGGGCTACCGCATcc                                                                                                                                                                                                                                                  <  1:890475/251‑1 (MQ=255) tCAACAAATTGTCCGAGTCGGCGAAGCTGCTTTCATAGGCTTTCAGGCTACGAATGAAGCTGTAAAATTCAGGCTCTTTACCAAAGGCATCGGCAAAGATTTTGGCTGCGGTCGCGTCGCCGTCACCGCGTAATTCCTGTGCGGTTTTTTCCGCATTGGCGATAATTAAGGTGACTTTACGATCTACATCCGCCTGAATAAATGCCGCTTTTTCTTTACCTTGAGAGCGGTGTTCACGGGCTACCGCATcc                                                                                                                                                                                                                                                  <  1:392646/251‑1 (MQ=255)      aaaTTGTCCGAGTCGGCGAAGCTGCTTTCATAGGCTTTCAGGCTACGAATGAAGCTGTAAAATTCAGGCTCTTTACCAAAGGCATCGGCAAAGATTTTGGCTGCGGTCGCGTCGCCGTCACCGCGTAATTCCTGTGCGGTTTTTTCCGCATTGGCGATAATTAAGGTGACTTTACGATCTACATCCGCCTGAATAAATGCCGCTTTTTCTTTACCTTGAGAGCGGTGTTCACGGGCTACCGCATCCCGTTc                                                                                                                                                                                                                                             <  1:26109/251‑1 (MQ=255)          tGTCCGAGTCGGCGAAGCTGCTTTCATAGGCTTTCAGGCTACGAATGAAGCTGTAAAATTCAGGCTCTTTACCAAAGGCATCGGCAAAGATTTTGGCTGCGGTCGCGTCGCCGTCACCGCGTAATTCCTGTGCGGTTTTTTCCGCATTGGCGATAATTAAGGTGACTTTACGATCTACATCCGCCTGAATAAATGCCGCTTTTTCTTTACCTTGAGAGCGGTGTTCACGGGCTACCGCATCCCGTTCGgcg                                                                                                                                                                                                                                         <  1:326944/251‑1 (MQ=255)                  tcggCGAAGCTGCTTTCATAGGCTTTCAGGCTACGAATGAAGCTGTAAAATTCAGGCTCTTTACCAAAGGCATCGGCAAAGATTTTGGCTGCGGTCGCGTCGCCGTCACCGCGTAATTCCTGTGCGGTTTTTTCCGCATTGGCGATAATTAAGGTGACTTTACGATCTACATCCGCCTGAATAAATGCCGCTTTTTCTTTACCTTGAGAGCGGTGTTCACGGGCTACCGCATCCCGTTCGGCGCGCATACg                                                                                                                                                                                                                                 <  1:24797/249‑1 (MQ=255)                   cggCGAAGCTGCTTTCATAGGCTTTCAGGCTACGAATGAAGCTGTAAAATTCAGGCTCTTTACCAAAGGCATCGGCAAAGATTTTGGCTGCGGTCGCGTCGCCGTCACCGCGTAATTCCTGTGCGGTTTTTTCCGCATTGGCGATAATTAAGGTGACTTTACGATCTACATCCGCCTGAATAAATGCCGCTTTTTCTTTACCTTGAGAGCGGTGTTCACGGGCTACCGCATCCCGTTCGGCGCGCATACGc                                                                                                                                                                                                                                <  1:807983/250‑1 (MQ=255)                             gctTTCATAGGCTTTCAGGCTACGAATGAAGCTGTAAAATTCAGGCTCTTTACCAAAGGCATCGGCAAAGATTTTGGCTGCGGTCGCGTCGCCGTCACCGCGTAATTCCTGTGCGGTTTTTTCCGCATTGGCGATAATTAAGGTGACTTTACGATCTACATCCGCCTGAATAAATGCCGCTTTTTCTTTACCTTGAGAGCGGTGTTCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATgg                                                                                                                                                                                                                      >  1:308135/1‑251 (MQ=255)                             gctTTCATAGGCTTTCAGGCTACGAATGAAGCTGTAAAATTCAGGCTCTTTACCAAAGGCATCGGCAAAGATTTTGGCTGCGGTCGCGTCGCCGTCACCGCGTAATTCCTGTGCGGTTTTTTCCGCATTGGCGATAATTAAGGTGACTTTACGATCTACATCCGCCTGAATAAATGCCGCTTTTTCTTTACCTTGAGAGCGGTGTTCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATgg                                                                                                                                                                                                                      >  1:313079/1‑251 (MQ=255)                              ctTTCATAGGCTTTCAGGCTACGAATGAAGCTGTAAAATTCAGGCTCTTTACCAAAGGCATCGGCAAAGATTTTGGCTGCGGTCGCGTCGCCGTCACCGCGTAATTCCTGTGCGGTTTTTTCCGCATTGGCGATAATTAAGGTGACTTTACGATCTACATCCGCCTGAATAAATGCCGCTTTTTCTTTACCTTGAGAGCGGTGTTCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATgg                                                                                                                                                                                                                      <  1:462000/250‑1 (MQ=255)                                ttCATAGGCTTTCAGGCTACGAATGAAGCTGTAAAATTCAGGCTCTTTACCAAAGGCATCGGCAAAGATTTTGGCTGCGGTCGCGTCGCCGTCACCGCGTAATTCCTGTGCGGTTTTTTCCGCATTGGCGATAATTAAGGTGACTTTACGATCTACATCCGCCTGAATAAATGCCGCTTTTTCTTTACCTTGAGAGCGGTGTTCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATggagg                                                                                                                                                                                                                   >  1:174130/1‑251 (MQ=255)                                               gCTACGAATGAAGCTGTAAAATTCAGGCTCTTTACCAAAGGCATCGGCAAAGATTTTGGCTGCGGTCGCGTCGCCGTCACCGCGTAATTCCTGTGCGGTTTTTTCCGCATTGGCGATAATTAAGGTGACTTTACGATCTACATCCGCCTGAATAAATGCCGCTTTTTCTTTACCTTGAGAGCGGTGTTCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATcc                                                                                                                                                                                                     <  1:146173/250‑1 (MQ=255)                                               gCTACGAATGAAGCTGTAAAATTCAGGCTCTTTACCAAAGGCATCGGCAAAGATTTTGGCTGCGGTCGCGTCGCCGTCACCGCGTAATTCCTGTGCGGTTTTTTCCGCATTGGCGATAATTAAGGTGACTTTACGATCTACATCCGCCTGAATAAATGCCGCTTTTTCTTTACCTTGAGAGCGGTGTTCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATc                                                                                                                                                                                                      <  1:866976/249‑1 (MQ=255)                                               gCTACGAATGAAGCTGTAAAATTCAGGCTCTTTACCAAAGGCATCGGCAAAGATTTTGGCTGCGGTCGCGTCGCCGTCACCGCGTAATTCCTGTGCGGTTTTTTCCGCATTGGCGATAATTAAGGTGACTTTACGATCTACATCCGCCTGAATAAATGCCGCTTTTTCTTTACCTTGAGAGCGGTGTTCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATc                                                                                                                                                                                                      <  1:143166/249‑1 (MQ=255)                                                 tACGAATGAAGCTGTAAAATTCAGGCTCTTTACCAAAGGCATCGGCAAAGATTTTGGCTGCGGTCGCGTCGCCGTCACCGCGTAATTCCTGTGCGGTTTTTTCCGCATTGGCGATAATTAAGGTGACTTTACGATCTACATCCGCCTGAATAAATGCCGCTTTTTCTTTACCTTGAGAGCGGTGTTCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATCCGGc                                                                                                                                                                                                  <  1:106498/251‑1 (MQ=255)                                                                 aaaTTCAGGCTCTTTACCAAAGGCATCGGCAAAGATTTTGGCTGCGGTCGCGTCGCCGTCACCGCGTAATTCCTGTGCGGTTTTTTCCGCATTGGCGATAATTAAGGTGACTTTACGATCTACATCCGCCTGAATAAATGCCGCTTTTTCTTTACCTTGAGAGCGGTGTTCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATCCGGCAAGTTAATTTGTTTaa                                                                                                                                                                                  >  1:311625/1‑251 (MQ=255)                                                                   aTTCAGGCTCTTTACCAAAGGCATCGGCAAAGATTTTGGCTGCGGTCGCGTCGCCGTCACCGCGTAATTCCTGTGCGGTTTTTTCCGCATTGGCGATAATTAAGGTGACTTTACGATCTACATCCGCCTGAATAAATGCCGCTTTTTCTTTACCTTGAGAGCGGTGTTCACGGGCTACCGCATcc                                                                                                                                                                                                                                                  >  1:47302/1‑185 (MQ=255)                                                                   aTTCAGGCTCTTTACCAAAGGCATCGGCAAAGATTTTGGCTGCGGTCGCGTCGCCGTCACCGCGTAATTCCTGTGCGGTTTTTTCCGCATTGGCGATAATTAAGGTGACTTTACGATCTACATCCGCCTGAATAAATGCCGCTTTTTCTTTACCTTGAGAGCGGTGTTCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATCCGGCAAGTTAATTTGTTTAATc                                                                                                                                                                                <  1:596476/251‑1 (MQ=255)                                                                         gCTCTTTACCAAAGGCATCGGCAAAGATTTTGGCTGCGGTCGCGTCGCCGTCACCGCGTAATTCCTGTGCGGTTTTTTCCGCATTGGCGATAATTAAGGTGACTTTACGATCTACATCCGCCTGAATAAATGCCGCTTTTTCTTTACCTTGAGAGCGGTGTTCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATCCGGCAAGTTAATTTGTTTAATCCGTACg                                                                                                                                                                          >  1:40451/1‑251 (MQ=255)                                                                          ctctTTACCAAAGGCATCGGCAAAGATTTTGGCTGCGGTCGCGTCGCCGTCACCGCGTAATTCCTGTGCGGTTTTTTCCGCATTGGCGATAATTAAGGTGACTTTACGATCTACATCCGCCTGAATAAATGCCGCTTTTTCTTTACCTTGAGAGCGGTGTTCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATCCGGCAAGTTAATTTGTTTAATCCGTACGt                                                                                                                                                                         >  1:522699/1‑251 (MQ=255)                                                                          ctctTTACCAAAGGCATCGGCAAAGATTTTGGCTGCGGTCGCGTCGCCGTCACCGCGTAATTCCTGTGCGGTTTTTTCCGCATTGGCGATAATTAAGGTGACTTTACGATCTACATCCGCCTGAATAAATGCCGCTTTTTCTTTACCTTGAGAGCGGTGTTCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATCCGGCAAGTTAATTTGTTTAATCCGTACGt                                                                                                                                                                         <  1:875190/251‑1 (MQ=255)                                                                                aCCAAAGGCATCGGCAAAGATTTTGGCTGCGGTCGCGTCGCCGTCACCGCGTAATTCCTGTGCGGTTTTTTCCGCATTGGCGATAATTAAGGTGACTTTACGATCTACATCCGCCTGAATAAATGCCGCTTTTTCTTTACCTTGAGAGCGGTGTTCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATCCGGCAAGTTAATTTGTTTAATCCGTACGTCGAGCa                                                                                                                                                                   >  1:671285/1‑251 (MQ=255)                                                                                                          cTGCGGTCGCGTCGCCGTCACCGCGTAATTCCTGTGCGGTTTTTTCCGCATTGGCGATAATTAAGGTGACTTTACGATCTACATCCGCCTGAATAAATGCCGCTTTTTCTTTACCTTGAGAGCGGTGTTCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATCCGGCAAGTTAATTTGTTTAATCCGTACGTCGAGCACTTCAATCCCCAGTTCGGCGGTg                                                                                                                                            >  1:804725/1‑248 (MQ=255)                                                                                                             cGGTCGCGTCGCCGTCACCGCGTAATTCCTGTGCGGTTTTTTCCGCATTGGCGATAATTAAGGTGACTTTACGATCTACATCCGCCTGAATAAATGCCGCTTTTTCTTTACCTTGAGAGCGGTGTTCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATCCGGCAAGTTAATTTGTTTAATCCGTACGTCGAGCACTTCAATCCCCAGTTCGGCGGTGCTGTCt                                                                                                                                      <  1:310521/251‑1 (MQ=255)                                                                                                                   cgtcgcCGTCACCGCGTAATTCCTGTGCGGTTTTTTCCGCATTGGCGATAATTAAGGTGACTTTACGATCTACATCCGCCTGAATAAATGCCGCTTTTTCTTTACCTTGAGAGCGGTGTTCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATCCGGCAAGTTAATTTGTTTAATCCGTACGTCGAGCACTTCAATcc                                                                                                                                                          >  1:243313/1‑225 (MQ=255)                                                                                                                        ccGTCACCGCGTAATTCCTGTGCGGTTTTTTCCGCATTGGCGATAATTAAGGTGACTTTACGATCTACATCCGCCTGAATAAATGCCGCTTTTTCTTTACCTTGAGAGCGGTGTTCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATCCGGCAAGTTAATTTGTTTAATCCGTACGTCGAGCACTTCAATCCCCAGTTCGGCGGTGCTGTCTTGCCCACTGt                                                                                                                            <  1:35102/250‑1 (MQ=255)                                                                                                                         cGTCACCGCGTAATTCCTGTGCGGTTTTTTCCGCATTGGCGATAATTAAGGTGACTTTACGATCTACATCCGCCTGAATAAATGCCGCTTTTTCTTTACCTTGAGAGCGGTGTTCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATCCGGCAAGTTAATTTGTTTAATCCGTACGTCGAGCACTTCAATCCCCAGTTCGGCGGTGCTGTCTTGCCCACTGTTg                                                                                                                          <  1:162244/251‑1 (MQ=255)                                                                                                                            cACCGCGTAATTCCTGTGCGGTTTTTTCCGCATTGGCGATAATTAAGGTGACTTTACGATCTACATCCGCCTGAATAAATGCCGCTTTTTCTTTACCTTGAGAGCGGTGTTCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATCCGGCAAGTTAATTTGTTTAATCCGTACGTCGAGCACTTCAATCCCCAGTTCGGCGGTGCTGTCTTGCCCACTGTTGAgc                                                                                                                       <  1:232706/251‑1 (MQ=255)                                                                                                                                                gTTTTTTCCGCATTGGCGATAATTAAGGTGACTTTACGATCTACATCCGCCTGAATAAATGCCGCTTTTTCTTTACCTTGAGAGCGGTGTTCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATgg                                                                                                                                                                                                                      <  1:199366/136‑1 (MQ=255)                                                                                                                                                 ttttttCCGCATTGGCGATAATTAAGGTGACTTTACGATCTACATCCGCCTGAATAAATGCCGCTTTTTCTTTACCTTGAGAGCGGTGTTCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATCCGGCAAGTTAATTTGTTTAATCCGTACGTCGAGCACTTCAATCCCCAGTTCGGCGGTGCTGTCTTGCCCACTGTTGAGCGCTTTTTTCGTGCCTACCATg                                                                                                  <  1:172845/251‑1 (MQ=255)                                                                                                                                                    tttCCGCATTGGCGATAATTAAGGTGACTTTACGATCTACATCCGCCTGAATAAATGCCGCTTTTTCTTTACCTTGAGAGCGGTGTTCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATCCGGCAAGTTAATTTGTTTAATCCGTACGTCGAGCACTTCaa                                                                                                                                                             <  1:72879/189‑1 (MQ=255)                                                                                                                                                    tttCCGCATTGGCGATAATTAAGGTGACTTTACGATCTACATCCGCCTGAATAAATGCCGCTTTTTCTTTACCTTGAGAGCGGTGTTCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATCCGGCAAGTTAATTTGTTTAATCCGTACGTCGAGCACTTCAATCCCCAGTTCGGCGGTGCTGTCTTGCCCACTGTTGAGCGCTTTTTTCGTGCCTACCATGAGt                                                                                               >  1:817503/1‑251 (MQ=255)                                                                                                                                                                   tAATTAAGGTGACTTTACGATCTACATCCGCCTGAATAAATGCCGCTTTTTCTTTACCTTGAGAGCGGTGTTCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATCCGGCAAGTTAATTTGTTTAATCCGTACGTCGAGCACTTCAATCCCCAGTTCGGCGGTGCTGTCTTGCCCACTGTTGAGCGCTTTTTTCGTGCCTACCATGAGtt                                                                                              <  1:670817/237‑1 (MQ=255)                                                                                                                                                                             gACTTTACGATCTACATCCGCCTGAATAAATGCCGCTTTTTCTTTACCTTGAGAGCGGTGTTCACGGGCTACCGCATCCCGTTCGGCGCGCATACg                                                                                                                                                                                                                                 >  1:700541/1‑96 (MQ=255)                                                                                                                                                                               cTTTACGATCTACATCCGCCTGAATAAATGCCGCTTTTTCTTTACCTTGAGAGCGGTGTTCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATCCGGCAAGTTAATTTGTTTAATCCGTACGTCGAGCACTTCAATCCCCAGTTCGGCGGTGCTGTCTTGc                                                                                                                                   <  1:682366/188‑1 (MQ=255)                                                                                                                                                                                      aTCTACATCCGCCTGAATAAATGCCGCTTTTTCTTTACCTTGAGAGCGGTGTTCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATCCGGCAAGTTAATTTGTTTAATCCGTACGTCGAGCACTTCAATCCCCAGTTCGGCGGTGCTGTCTTGCCCACTGt                                                                                                                            >  1:388820/1‑188 (MQ=255)                                                                                                                                                                                      aTCTACATCCGCCTGAATAAATGCCGCTTTTTCTTTACCTTGAGAGCGGTGTTCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATCCGGCAAGTTAATTTGTTTAATCCGTACGTCGAGCACTTCAATCCCCAGTTCGGCGGTGCTGTCTTGCCCACTGTTGAGCGCTTTTTTCGTGCCTACCATGAGTTCGCCTCGTGTACCGGAAACGATGTCTTTAA‑Tgg                                                            <  1:874875/251‑1 (MQ=255)                                                                                                                                                                                      aTCTACATCCGCCTGAATAAATGCCGCTTTTTCTTTACCTTGAGAGCGGTGTTCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATCCGGCAAGTTAATTTGTTTAATCCGTACGTCGAGCACTTCAATCCCCAGTTCGGCGGTGCTGTCTTGCCCACTGTTGAGCGCTTTTTTCGTGCCTACCATGAGTTCGCCTCGTGTACCGGAAACGATGTCTTTAA‑Tgg                                                            >  1:481126/1‑251 (MQ=255)                                                                                                                                                                                      aTCTACATCCGCCTGAATAAATGCCGCTTTTTCTTTACCTTGAGAGCGGTGTGCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATCCGGCAAGTTAATTTGTTTAATCCGTACGGCGAGCACTTCAATCCCCAGTTCGGCGGTGCTGTCTTGCCCACTGTTGAGCGCTTTTTTCGTGCCTACCATGAGTTCGcc                                                                                          <  1:455224/222‑1 (MQ=255)                                                                                                                                                                                      aTCTACATCCGCCTGAATAAATGCCGCTTTTTCTTTACCTTGAGAGCGGAGTTCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATCCGGCAAGTTAATTTGTTTAATCCGTACGTCGAGCACTTCAATCCCCAGTTCGGCGGTGCTGTCTTGCCCACTGTTGAGCGCTTTTTTCGTGCCTACCATGAGTTCGCCTCGTGTACCGGAAACGATGTCTTTAA‑Tgg                                                            <  1:542939/251‑1 (MQ=255)                                                                                                                                                                                        cTACATCCGCCTGAATAAATGCCGCTTTTTCTTTACCTTGAGAGCGGTGTTCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATCCGGCAAGTTAATTTGTTTAATCCGTACGTCGAGCACTTCAATCCCCAGTTCGGCGGTGCTGTCTTGCCCACTGTTGAGCGCTTTTTTCGTGCCTACCATGAGTTCGCCTCGTGTACCGGAAACGATGTCTTTAA‑Tgg                                                            <  1:113666/249‑1 (MQ=255)                                                                                                                                                                                          aCATCCGCCTGAATAAATGCCGCTTTTTCTTTACCTTGAGAGCGGTGTTCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATCCGGCAAGTTAATTTGTTTAATCCGTACGTCGAGCACTTCAATcc                                                                                                                                                          >  1:500236/1‑154 (MQ=255)                                                                                                                                                                                          aCATCCGCCTGAATAAATGCCGCTTTTTCTTTACCTTGAGAGCGGTGTTCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATCCGGCAAGTTAATTTGTTTAATCCGTACGTCGAGCACTTCAATCCCCAGTTCGGCGGTGCTGTCTTGCCCACTGTTGAgc                                                                                                                       <  1:184620/189‑1 (MQ=255)                                                                                                                                                                                              ccGCCTGAATAAATGCCGCTTTTTCTTTACCTTGAGAGCGGTGTTCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATCCGGCAAGTTAATTTGTTTAATCCGTACGTCGAGCACTTCAATCCCCAGTTCGGCGGTGCTGTCTTGCCCACTGTTGAGCGCTTTTTTCGTGCCTACCATGAGTTCGCCTCGTGTACCGGAAACGATGTCTTTAA‑TGGTGCGGGaa                                                    >  1:836634/1‑251 (MQ=255)                                                                                                                                                                                               cGCCTGAATAAATGCCGCTTTTTCTTTACCTTGAGAGCGGTGTTCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATCCGGCAAGTTAATTTGTTTAATCCGTACGt                                                                                                                                                                         <  1:543269/134‑1 (MQ=255)                                                                                                                                                                                                         aaTGCCGCTTTTTCTTTACCTTGAGAGCGGTGTTCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATCCGGCAAGTTAATTTGTTTAATCCGTACGTCGAGCACTTCAATCCCCAGTTCGGCGGTGCTGTCTTGCCCACTGTTGAGCGCTTTTTTCGTGCCTACCATGAGTTCGCCTCGTGTACCGGAAACGATGTCTTTAA‑TGGTGCGGGAACCGATTTCGGa                                         <  1:28691/251‑1 (MQ=255)                                                                                                                                                                                                                   tttCTTTACCTTGAGAGCGGTGTTCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATCCGGCAAGTTAATTTGTTTAATCCGTACGTCGAGAACTTCAATCCCCAGTTCGGCGGTGCTGTCTTGCCCACTGTTGAGCGCTTTTTTCGTGCCTACCATGAGTTCGCCTCGTGTACCGGAAACGATGTCTTTAA‑TGGTGCGGGAACCGATTTCGGAACGCAAACgg                               >  1:124705/1‑251 (MQ=255)                                                                                                                                                                                                                           ccTTGAGAGCGGTGTTCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATCCGGCAAGTTAATTTGTTTAATCCGTACGTCGAGCACTTCAATCCCCAGTTCGGCGGTGCTGTCTTGCCCACTGTTGAGCGCTTTTTTCGTGCCTACCATGAGTTCGCCTCGTGTACCGGAAACGATGTCTTTAA‑TGGTGCGGGAACCGATTTCGGAACGCAAACGGTCATTGa                        >  1:48065/1‑250 (MQ=255)                                                                                                                                                                                                                                    cGGTGTTCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATCCGGCAAGTTAATTTGTTTAATCCGTACGTCGAGCACTTCAATCCCCAGTTCGGCGGTGCTGTCTTGCCCACTGTTGAGCGCTTTTTTCGTGCCTACCATGAGTTCGCCTCGTGTACCGGAAACGATGTCTTTAA‑TGGTGCGGGAACCGATTTCGGAACGCAAACGGTCATTGACTTTacgac               <  1:253311/250‑1 (MQ=255)                                                                                                                                                                                                                                    cGGTGTTCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATCCGGCAAGTTAATTTGTTTAATCCGTACGTCGAGCACTTCAATCCCCAGTTCGGCGGTGCTGTCTTGCCCACTGTTGAGCGCTTTTTTCGTGCCTACCATGAGTTCGCCTCGTGTACCGGAAACGATGTCTTTAA‑TGGTGCGGGAACCGATTTCGGAACGCAAACGGTCATTGACTTTacga                <  1:158137/249‑1 (MQ=255)                                                                                                                                                                                                                                      gtgtTCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATCCGGCAAGTTAATTTGTTTAATc                                                                                                                                                                                <  1:703958/88‑1 (MQ=255)                                                                                                                                                                                                                                      gtgtTCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATCCGGCAAGTTAATTTGTTTAATCCGTACGTCGAGCACTTCAATCCCCAGTTCGGCGGTGCTGTCTTGCCCACTGTTGAGCGCTTTTTTCGTGCCTACCATGAGTTCGCCTCGTGTACCGGAAACGATGTCTTTAA‑TGGTGCGGGAACCGATTTCGGAACGCAAACGGTCATTGACTTTACGACGta            >  1:664474/1‑251 (MQ=255)                                                                                                                                                                                                                                      gtgtTCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATCCGGCAAGTTAATTTGTTTAATCCGTACGTCGAGCACTTCAATCCCCAGTTCGGCGGTGCTGTCTTGCCCACTGTTGAGCGCTTTTTTCGTGCCTACCATGAGTTCGCCTCGTGTACCGGAAACGATGTCTTTAA‑TGGTGCGGGAACCGATTTCGGAACGCAAACGGTCATTGACTTTACGACGta            >  1:642713/1‑251 (MQ=255)                                                                                                                                                                                                                                        gtTCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATCCGGCAAGTTAATTTGTTTAATc                                                                                                                                                                                >  1:166885/1‑86 (MQ=255)                                                                                                                                                                                                                                        gtTCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATCCGGCAAGTTAATTTGTTTAATCCGTACGTCGAGCACTTCAATCCCCAGTTCGGCGGTGCTGTCTTGCCCACTGTTGAGCGCTTTTTTCGTGCCTACCATGAGTTCGCCTCGTGTACCGGAAACGATGTCTTTAA‑Tgg                                                            <  1:532263/201‑1 (MQ=255)                                                                                                                                                                                                                                        gtTCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATCCGGCAAGTTAATTTGTTTAATCCGTACGTCGAGCACTTCAATCCCCAGTTCGGCGGTGCTGTCTTGCCCACTGTTGAGCGCTTTTTTCGTGCCTACCATGAGTTCGCCTCGTGTACCGGAAACGATGTCTTTAA‑TGGTGCGGGAACCGATTTCGGAACGCAAACGGTCATTGACTTTac                  >  1:224618/1‑243 (MQ=255)                                                                                                                                                                                                                                        gtTCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATCCGGCAAGTTAATTTGTTTAATCCGTACGTCGAGCACTTCAATCCCCAGTTCGGCGGTGCTGTCTTGCCCACTGTTGAGCGCTTTTTTCGTGCCTACCATGAGTTCGCCTCGTGTACCGGAAACGATGTCTTTAA‑TGGTGCGGGAACCGATTTCGGAACGCAAACGGTCATTGACTTTACGACGtaat          >  1:419286/1‑251 (MQ=255)                                                                                                                                                                                                                                        gtTCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATCCGGCAAGTTAATTTGTTTAATCCGTACGTCGAGCACTTCAATCCCCAGTTCGGCGGTGCTGTCTTGCCCACTGTTGAGCGCTTTTTTCGTGCCTACCATGAGTTCGCCTCGTGTACCGGAAACGATGTCTTTAA‑TGGTGCGGGAACCGATTTCGGAACGCAAACGGTCATTGACTTTACGACGtaat          >  1:248341/1‑251 (MQ=255)                                                                                                                                                                                                                                        gtTCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATCCGGCAAGTTAATTTGTTTAATCCGTACGTCGAGCACTTCAATCCCCAGTTCGGCGGTGCTGTCTTGCCCACTGTTGAGCGCTTTTTTCGTGCCTACCATGAGTTCACCTCGTGTACCGGAAACGATGTCTTTAA‑TGGTGCGGGAACCGACTTCGGAACGCAAACGGTCATTGACTTTACGACGtaat          >  1:456289/1‑251 (MQ=255)                                                                                                                                                                                                                                          tCACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATCCGGCAAGTTAATTTGTTTAATCCGTACGTCGAGCACTTCAATCCCCAGTTCGGCGGTGCTGTCTTGCCCACTGTTGAGCGCTTTTTTCGTGCCTACCATGAGTTCGCCTCGTGTACCGGAAACGATGTCTTTAA‑TGGTGCGGGAACCGATTTCGGAACGCAAACGGTCATTGACTTTACGACGtaataa        <  1:411347/251‑1 (MQ=255)                                                                                                                                                                                                                                           cACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATCCGGCAAGTTAATTTGTTTAATCCGTACGTCGAGCACTTCAATCCCCAGTTCGGCGGTGCTGTCTTGCCCACTGTTGAGCGCTTTTTTCGTGCCTACCATGAGTTCGCCTCGTGTACCGGAAACGATGTCTTTAA‑TGGTGCGGGAACCGATTTCGGAACGCAAACGGTCATTGACTTTACGACGTAATAAg       <  1:660377/251‑1 (MQ=255)                                                                                                                                                                                                                                           cACGGGCTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATCCGGCAAGTTAATTTGTTTAATCCGTACGTCGAGCACTTCAATCCCCAGTTCGGCGGTGCTGTCTTGCCCACTGTTGAGCGCTTTTTTCGTGCCTACCATGAGTTCGCCTCGTGTACCGGAAACGATGTCTTTAA‑TGGTGCGGGAACCGATTTCGGAACGCAAACGGTCATTGACTTTACGACGTAATAAg       <  1:233130/251‑1 (MQ=255)                                                                                                                                                                                                                                                 cTACCGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATCCGGCAAGTTAATTTGTTTAATCCGTACGTCGAGCACTTCAATCCCCAGTTCGGCGGTGCTGTCTTGCCCACTGTTGAGCGCTTTTTTCGTGCCTACCATGAGTTCGCCTCGTGTACCGGAAACGATGTCTTTAATTGGTGCGGGAACCGATTTCGGAACGCAAACGGTCATTGACTTTACGACGTAATAAGTTGgc  <  1:825167/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                       TCAACAAATTGTCCGAGTTGGCGAAGCTGCTTTCATAGGCTTTCAGGCTACGAATGAAGCTGTAAAATTCAGGCTCTTTACCAAAGGCATCGGCAAAGATTTTGGCTGCGGTCGCGTCGCCGTCACCGCGTAATTCCTGTGCGGTTTTTTCCGCATTGGCGATAATTAAGGTGACTTTACGATCTACATCCGCCTGAATAAATGCCGCTTTTTCTTTACCTTGAGAGCGGTGTTCACGGGCTACTGCATCCCGTTCGGCGCGCATACGCTGGTAAATGGAGGAAGACACTTCATCCGGCAAGTTAATTTGTTTAATCCGTACGTCGAGCACTTCAATCCCCAGTTCGGCGGTGCTGTCTTGCCCACTGTTGAGCGCTTTTTTCGTGCCTACCATGAGTTCGCCTCGTGTACCGGAAACGATGTCTTTAA‑TGGTGCGGGAACCGATTTCGGAACGCAAACGGTCATTGACTTTACGACGTAATAAGTTGGC  >  1/1080189‑1080678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑