Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,059,227	T→C	intergenic (+18304/+237)	unknown → / ← unknown	tRNA‑Trp‑CCA/tRNA‑Ser‑GGA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,059,227	0	T	C	100.0%	235.7 / NA	82	intergenic (+18304/+237)	unknown/unknown	tRNA‑Trp‑CCA/tRNA‑Ser‑GGA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (39/43);  total (39/43)

CACTTTTACTGTGCCGTCCGGCAACTTTAGCAGCTGAATAATGTTGGCAATAGTGCCCACATCATATAAATCTTCAATGCCCGGCTCTTCCAATTCCGCCTGTTTTTGCGAAACCAACAACAGCTGTTTTTCATTATTCATGGCTTCGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGTAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTGGGATGGATTATGTTAATTCAAGACCTAAAACAGATATGCCGGCACAGAAAAGTGCGGTCGTTTTTTTCAGTGTTTTTTATTATTGAAACGGCTGTCAGTATAACCGAAATTGATTGGTGTAAGGCAAAATAAAATTCATTAAGAATGGCGG  >  1/1059005‑1059469                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                   cacTTTTACTGTGCCGTCCGGCAACTTTAGCAGCTGAATAATGTTGGCAATAGTGCCCAAATCATATAAATCTTCAATGCCCGGCTCTTCCAATTCCGCCTGTTTTTGCGAAACCAACAACAGCTGTTTTTCATTATTCATGGCTTCGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACGACATCGCGCAATGGCAATACCGGTATGGTTTGTaa                                                                                                                                                                                                                          >  1:19285/1‑249 (MQ=255) cacTTTTACTGTGCCGTCCGGCAACTTTAGCAGCTGAATAATGTTGGCAATAGTGCCCAAATCATATAAATCTTCAATGCCCGGCTCTTCCAATTCCGCCTGTTTTTGCGAAACCAACAACAGCTGTTTTTCATTATTCATGGCTTCGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAAtt                                                                                                                                                                                                                        >  1:24651/1‑251 (MQ=255) cacTTTTACTGTGCCGTCCGGCAACTTTAGCAGCTGAATAATGTTGGCAATAGTGCCCAAATCATATAAATCTTCAATGCCCGGCTCTTCCAATTCCGCCTGTTTTTGCGAAACCAACAACAGCTGTTTTTCATTATTCATGGCTTCGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAAt                                                                                                                                                                                                                         >  1:409312/1‑250 (MQ=255)      ttACTGTGCCGTCCGGCAACTTTAGCAGCTGAATAATGTTGGCAATAGTGCCCAAATCATATAAATCTTCAATGCCCGGCTCTTCCAATTCCGCCTGTTTTTGCGAAACCAACAACAGCTGTTTTTCATTATTCATGGCTTCGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGAt                                                                                                                                                                                                                   >  1:765035/1‑251 (MQ=255)      ttACTGTGCCGTCCGGCAACTTTAGCAGCTGAATAATGTTGGCAATAGTGCCCAAATCATATAAATCTTCAATGCCCGGCTCTTCCAATTCCGCCTGTTTTTGCGAAACCAACAACAGCTGTTTTTCATTATTCATGGCTTCGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGAt                                                                                                                                                                                                                   >  1:635817/1‑251 (MQ=255)      ttACTGTGCCGTCCGGCAACTTTAGCAGCTGAATAATGTTGGCAATAGTGCCCAAATCATATAAATCTTCAATGCCCGGCTCTTCCAATTCCGCCTGTTTTTGCGAAACCAACAACAGCTGTTTTTCATTATTCATGGCTTCGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGAt                                                                                                                                                                                                                   >  1:640172/1‑251 (MQ=255)                 tCCGGCAACTTTAGCAGCTGAATAATGTTGGCAATAGTGCCCAAATCATATAAATCTTCAATGCCCGGCTCTTCCAATTCCGCCTGTTTTTGCGAAACCAACAACAGCTGTTTTTCATTATTCATGGCTTCGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGctc                                                                                                                                                                                                         <  1:791595/250‑1 (MQ=255)                      cAACTTTAGCAGCTGAATAATGTTGGCAATAGTGCCCAAATCATATAAATCTTCAATGCCCGGCTCTTCCAATTCCGCCTGTTTTTGCGAAACCAACAACAGCTGTTTTTCATTATTCATGGCTTCGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCtcatc                                                                                                                                                                                                    <  1:784341/250‑1 (MQ=255)                        aCTTTAGCAGCTGAATAATGTTGGCAATAGTGCCCAAATCATATAAATCTTCAATGCCCGGCTCTTCCAATTCCGCCTGTTTTTGCGAAACCAACAACAGCTGTTTTTCATTATTCATGGCTTCGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATg                                                                                                                                                                                                 >  1:219861/1‑251 (MQ=255)                         cTTTAGCAGCTGAATAATGTTGGCAATAGTGCCCAAATCATATAAATCTTCAATGCCCGGCTCTTCCAATTCCGCCTGTTTTTGCGAAACCAACAACAGCTGTTTTTCATTATTCATGGCTTCGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGt                                                                                                                                                                                                >  1:134745/1‑251 (MQ=255)                           ttAGCAGCTGAATAATGTTGGCAATAGTGCCCAAATCATATAAATCTTCAATGCCCGGCTCTTCCAATTCCGCCTGTTTTTGCGAAACCAACAACAGCTGTTTTTCATTATTCATGGCTTCGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTAc                                                                                                                                                                                              <  1:387739/251‑1 (MQ=255)                                    gAATAATGTTGGCAATAGTGCCCAAATCATATAAATCTTCAATGCCCGGCTCTTCCAATTCCGCCTGTTTTTGCGAAACCAACAACAGCTGTTTTTCATTATTCATGGCTTCGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATgg                                                                                                                                                                                                                                               <  1:184498/193‑1 (MQ=255)                                    gAATAATGTTGGCAATAGTGCCCAAATCATATAAATCTTCAATGCCCGGCTCTTCCAATTCCGCCTGTTTTTGCGAAACCAACAACAGCTGTTTTTCATTATTCATGGCTTCGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTAt                                                                                                                                                                                      >  1:774918/1‑250 (MQ=255)                                    gAATAATGTTGGCAATAGTGCCCAAATCATATAAATCTTCAATGCCCGGCTCTTCCAATTCCGCCTGTTTTTGCGAAACCAACAACAGCTGTTTTTCATTATTCATGGCTTCGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTAt                                                                                                                                                                                      >  1:7049/1‑250 (MQ=255)                                    gAATAATGTTGGCAATAGTGCCCAAATCATATAAATCTTCAATGCCCGGCTCTTCCAATTCCGCCTGTTTTTGCGAAACCAACAACAGCTGTTTTTCATTATTCATGGCTTCGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTAt                                                                                                                                                                                      >  1:138069/1‑250 (MQ=255)                                     aataatGTTGGCAATAGTGCCCAAATCATATAAATCTTCAATGCCCGGCTCTTCCAATTCCGCCTGTTTTTGCGAAACCAACAACAGCTGTTTTTCATTATTCATGGCTTCGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCt                                                                                                                                                                                    <  1:130715/251‑1 (MQ=255)                                         atGTTGGCAATAGTGCCCAAATCATATAAATCTTCAATGCCCGGCTCTTCCAATTCCGCCTGTTTTTGCGAAACCAACAACAGCTGTTTTTCATTATTCATGGCTTCGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAAt                                                                                                                                                                                <  1:20499/251‑1 (MQ=255)                                            ttGGCAATAGTGCCCAAATCATATAAATCTTCAATGCCCGGCTCTTCCAATTCCGCCTGTTTTTGCGAAACCAACAACAGCTGTTTTTCATTATTCATGGCTTCGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTc                                                                                                                                                                              <  1:66442/250‑1 (MQ=255)                                            ttGGCAATAGTGCCCAAATCATATAAATCTTCAATGCCCGGCTCTTCCAATTCCGCCTGTTTTTGCGAAACCAACAACAGCTGTTTTTCATTATTCATGGCTTCGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTc                                                                                                                                                                              <  1:828998/250‑1 (MQ=255)                                                       gCCCAAATCATATAAATCTTCAATGCCCGGCTCTTCCAATTCCGCCTGTTTTTGCGAAACCACCAACAGCTGTTTTTCATTATTCATGGCTTAGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTAATCATGTa                                                                                                                                                                                               <  1:389559/222‑1 (MQ=255)                                                       gCCCAAATCATATAAATCTTCAATGCCCGGCTCTTCCAATTCCGCCTGTTTTTGCGAAACCAACAACAGCTGTTTTTCATTATTCATGGCTTCGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCtggcttgg                                                                                                                                                                   >  1:251198/1‑250 (MQ=255)                                                             atcatATAAATCTTCAATGCCCGGCTCTTCCAATTCCGCCTGTTTTTGCGAAACCAACAACAGCTGTTTTTCATTATTCATGGCTTCGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTActc                                                                                                                                                                                            <  1:845245/219‑1 (MQ=255)                                                              tcatATAAATCTTCAATGCCCGGCTCTTCCAATTCCGCCTGTTTTTGCGAAACCAACAACAGCTGTTTTTCATTATTCATGGCTTCGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTatat                                                                                                                                                           <  1:619092/251‑1 (MQ=255)                                                                atatAAATCTTCAATGCCCGGCTCTTCCAATTCCGCCTGTTTTTGCGAAACCAACAACAGCTGTTTTTCATTATTCATGGCTTCGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATgg                                                                                                                                                                                                                                 >  1:541640/1‑179 (MQ=255)                                                                 tataAATCTTCAATGCCCGGCTCTTCCAATTCCGCCTGTTTTTGCGAAACCAACAACAGCTGTTTTTCATTATTCATGGCTTCGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTgg                                                                                                                                                        <  1:404911/251‑1 (MQ=255)                                                                   taAATCTTCAATGCCCGGCTCTTCCAATTCCGCCTGTTTTTGCGAAACCAACAACAGCTGTTTTTCATTATTCATGGCTTCGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTGgga                                                                                                                                                      <  1:821781/251‑1 (MQ=255)                                                                   taAATCTTCAATGCCCGGCTCTTCCAATTCCGCCTGTTTTTGCGAAACCAACAACAGCTGTTTTTCATTATTCATGGCTTCGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTGgga                                                                                                                                                      <  1:550809/251‑1 (MQ=255)                                                                   taAATCTTCAATGCCCGGCTCTTCCAATTCCGCCTGTTTTTGCGAAACCAACAACAGCTGTTTTTCATTATTCATGGCTTCGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTGgga                                                                                                                                                      <  1:440813/251‑1 (MQ=255)                                                                   taAATCTTCAATGCCCGGCTCTTCCAATTCCGCCTGTTTTTGCGAAACCAACAACAGCTGTTTTTCATTATTCATGGCTTCGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTGgga                                                                                                                                                      <  1:735886/251‑1 (MQ=255)                                                                      aTCTTCAATGCCCGGCTCTTCCAATTCCGCCTGTTTTTGCGAAACCAACAACAGCTGTTTTTCATTATTCATGGCTTCGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTGggatgg                                                                                                                                                   >  1:200/1‑251 (MQ=255)                                                                      aTCTTCAATGCCCGGCTCTTCCAATTCCGCCTGTTTTTGCGAAACCAACAACAGCTGTTTTTCATTATTCATGGCTTCGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTGgga                                                                                                                                                      <  1:684705/248‑1 (MQ=255)                                                                      aTCTTCAATGCCCGGCTCTTCCAATTCCGCCTGTTTTTGCGAAACCAACAACAGCTGTTTTTCATTATTCATGGCTTCGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTGgga                                                                                                                                                      >  1:88013/1‑248 (MQ=255)                                                                        cTTCAATGCCCGGCTCTTCCAATTCCGCCTGTTTTTGCGAAACCAACAACAGCTGTTTTTCATTATTCATGGCTTCGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTGgga                                                                                                                                                      >  1:695637/1‑246 (MQ=255)                                                                             aTGCCCGGCTCTTCCAATTCCGCCTGTTTTTGCGAAACCAACAACAGCTGTTTTTCATTATTCATGGCTTCGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTGGGATGGATTATGt                                                                                                                                            <  1:298514/251‑1 (MQ=255)                                                                                    gCTCTTCCAATTCCGCCTGTTTTTGCGAAACCAACAACAGCTGTTTTTCATTATTCATGGCTTCGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTGGGATGGATTATGTTAATTc                                                                                                                                      <  1:415519/250‑1 (MQ=255)                                                                                       ctTCCAATTCCGCCTGTTTTTGCGAAACCAACAACAGCTGTTTTTCATTATTCATGGCTTCGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTGGGATGGATTATGtt                                                                                                                                           >  1:109385/1‑242 (MQ=255)                                                                                       ctTCCAATTCCGCCTGTTTTTGCGAAACCAACAACAGCTGTTTTTCATTATTCATGGCTTCGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTGGGATGGATTATGTTAATTCAAGa                                                                                                                                  >  1:723516/1‑251 (MQ=255)                                                                                               tCCGCCTGTTTTTGCGAAACCAACAACAGCTGTTTTTCATTATTCATGGCTTCGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTGGGATGGATTATGTTAATTCAAGACCTAAAAc                                                                                                                          <  1:91334/251‑1 (MQ=255)                                                                                                                      caacaGCTGTTTTTCATTATTCATGGCTTCGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTGGGATGGATTATGt                                                                                                                                            >  1:382892/1‑210 (MQ=255)                                                                                                                      caacaGCTGTTTTTCATTATTCATGGCTTCGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTGGGATGGATTATGTTAATTCAAGACCTAAAACAGATATGCCGGCACAGAAAAGTg                                                                                                   >  1:332720/1‑251 (MQ=255)                                                                                                                        acaGCTGTTTTTCATTATTCATGGCTTCGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCt                                                                                                                                                                                    <  1:367780/168‑1 (MQ=255)                                                                                                                         caGCTGTTTTTCATTATTCATGGCTTCGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTGGGATGGATTATGTTAATTCAAGACCTAAAACAGATATGCCGGCACAGAAAAGTGCgg                                                                                                >  1:833026/1‑251 (MQ=255)                                                                                                                              gTTTTTCATTATTCATGGCTTCGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTGGGATGGATTATGTTAATTCAAGACCTAAAACAGATATGCCGGCACAGAAAAGTGc                                                                                                  >  1:618270/1‑244 (MQ=255)                                                                                                                              gTTTTTCATTATTCATGGCTTCGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTGGGATGGATTATGTTAATTCAAGACCTAAAACAGATATGCCGGCACAGAAAAGTGCGGTCGtt                                                                                           >  1:265931/1‑251 (MQ=255)                                                                                                                                   tCATTATTCATGGCTTCGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATc                                                                                                                                                                                     <  1:865760/156‑1 (MQ=255)                                                                                                                                        attCATGGCTTCGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTGGGATGGATTATGTTAATTCAAGACCTAAAACAGATATGCCGGCACAGAAAAGTGCGGTCGTTTTTTTCAgtg                                                                                 >  1:352860/1‑251 (MQ=255)                                                                                                                                         ttCATGGCTTCGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTGGGATGGATTATGTTAATTCAAGACCTAAAACAGATATGCCGGCACAGAAAAGTGCGGTCGTTTTTTTCAgtgt                                                                                >  1:469456/1‑251 (MQ=255)                                                                                                                                          tCATGGCTTCGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTGGGATGGATTATGTTAATTCAAGACCTAAAACAGATATGCCGGCACAGAAAAGTGCGGTCGTTTTTTTCAGTGtt                                                                               >  1:815566/1‑251 (MQ=255)                                                                                                                                                cTTCGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTGGGATGGATTATGTTAATTCAAGACCTAAAACAGATATGCCGGCACAGAAAAGTGCGGTCGTTTTTTTCAGTGTTTTttat                                                                         <  1:167502/251‑1 (MQ=255)                                                                                                                                                    gTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTGgga                                                                                                                                                      <  1:279935/170‑1 (MQ=255)                                                                                                                                                        aGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTatat                                                                                                                                                           <  1:558031/161‑1 (MQ=255)                                                                                                                                                          aCTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAAt                                                                                                                                                                                                                         <  1:33544/97‑1 (MQ=255)                                                                                                                                                          aCTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTGGGATGGATTATGTTAATTCAAGACCTAAAACAGATATGCCGGCACAGAAAAGTGCGGTCGTTTTTTTCAGTGTTTTTTATTATTGaaa                                                                 <  1:287612/249‑1 (MQ=255)                                                                                                                                                           cTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCtggcttggct                                                                                                                                                                 <  1:761031/152‑1 (MQ=255)                                                                                                                                                           cTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTGGGATGGATTATGTTAATTCAAGACCTAAAACAGATATGCCGGCACAGAAAAGTGCGGTCGTTTTTTTCAGTGTTTTTTATTATTGAAACgg                                                              >  1:76223/1‑251 (MQ=255)                                                                                                                                                             gCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCt                                                                                                                                                                                    <  1:324901/131‑1 (MQ=255)                                                                                                                                                             gCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTGGGATGGATTATGTTAATTCAAGACCTAAAACAGATATGCCGGCACAGAAAAGTGCGGTCGTTTTTTTCAGTGTTTTTTATTATTGAAACgg                                                              >  1:156207/1‑249 (MQ=255)                                                                                                                                                              cTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTGGGATGGATTATGtt                                                                                                                                           >  1:371437/1‑171 (MQ=255)                                                                                                                                                                         cgGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTGGGATGGATTATGTTAATTCAAGACCTAAAACAGATATGCCGGCACAGAAAAGTGCGGTCGTTTTTTTCAGTGTTTTTTATTATTGAAACGGCTGTCAGTATAAc                                                 >  1:467241/1‑250 (MQ=255)                                                                                                                                                                                ccGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTaa                                                                                                                                                                                  >  1:202744/1‑114 (MQ=255)                                                                                                                                                                                   aCAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTGGGATGGATTATGTTAATTCAAGACCTAAAACAGATATGCCGGCACAGAAAAGTGCGGTCGTTTTTTTCAGTGTTTTTTATTATTGAAACGGCTGTCAGTATAACCGAAattgatt                                      <  1:228933/251‑1 (MQ=255)                                                                                                                                                                                   aCAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTGGGATGGATTATGTTAATTCAAGACCTAAAACAGATATGCCGGCACAGAAAAGTGCGGTCGTTTTTTTCAGTGTTTTTTATTATTGAAACGGCTGTCAGTATAACCGAAattgat                                       <  1:34389/250‑1 (MQ=255)                                                                                                                                                                                        tAACGGCATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTGGGATGGATTATGTTAATTCAAGACCTAAAACAGATATGCCGGCACAGAAAAGTGCGGTCGTTTTTTTCAGTGTTTTTTATTATTGAAACGGCTGTCAGTATAACCGAAATTGATTGgtgt                                 <  1:128482/251‑1 (MQ=255)                                                                                                                                                                                              cATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTGGGATGGATTATGTTAATTCAAGACCTAAAACAGATATGCCGGCACAGAAAAGTGCGGTCGTTTTTTTCAGTGTTTTTTATTATTGAAACGGCTGTCAGTATAACCGAAATTGATTGGTGTAAGGc                            <  1:580448/250‑1 (MQ=255)                                                                                                                                                                                              cATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTGGGATGGATTATGTTAATTCAAGACCTAAAACAGATATGCCGGCACAGAAAAGTGCGGTCGTTTTTTTCAGTGTTTTTTATTATTGAAACGGCTGTCAGTATAACCGAAATTGATTGGTGTAAGGc                            <  1:91431/250‑1 (MQ=255)                                                                                                                                                                                              cATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTGGGATGGATTATGTTAATTCAAGACCTAAAACAGATATGCCGGCACAGAAAAGTGCGGTCGTTTTTTTCAGTGTTTTTTATTATTGAAACGGCTGTCAGTATAACCGAAATTGATTGGTGTAAGGc                            <  1:508063/250‑1 (MQ=255)                                                                                                                                                                                              cATCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCAGCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTGGGATGGATTATGTTAATTCAAGACCTAAAACAGATATGCCGGCACAGAAAAGTGCGGTCGTTTTTTTCAGTGTTTTTTATTATTGAAACGGCTGTCAGTATAACCGAAATTGATTGGTGTAAGGc                            <  1:431034/250‑1 (MQ=255)                                                                                                                                                                                               aTCACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTGGGATGGATTATGTTAATTCAAGACCTAAAACAGATATGCCGGCACAGAAAAGTGCGGTCGTTTTTTTCAGTGTTTTTTATTATTGAAACGGCTGTCAGTATAACCGAAATTGATTGGTGTAAGGc                            >  1:170707/1‑249 (MQ=255)                                                                                                                                                                                                 cACCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTaa                                                                                                                                                                                                                          >  1:492326/1‑57 (MQ=255)                                                                                                                                                                                                  aCCATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTGGGATGGATTATGTTAATTCAAGACCTAAAACAGATATGCCGGCACAGAAAAGTGCGGTCGTTTTTTTCAGTGTTTTTTATTATTGAAACGGCTGTCAGTATAACCGAAATTGATTGGTGTAAGGCaaaat                       >  1:185645/1‑251 (MQ=255)                                                                                                                                                                                                   ccATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTatat                                                                                                                                                           <  1:601442/118‑1 (MQ=255)                                                                                                                                                                                                   ccATGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTGGGATGGATTATGTTAATTCAAGACCTAAAACAGATATGCCGGCACAGAAAAGTGCGGTCGTTTTTTTCAGTGTTTTTTATTATTGAAACGGCTGTCAGTATAACCGAAATTGATTGGTGTAAGGc                            <  1:877442/245‑1 (MQ=255)                                                                                                                                                                                                     aTGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTGGGATGGATTATGTTAATTCAAGACCTAAAACAGATATGc                                                                                                                  <  1:571265/157‑1 (MQ=255)                                                                                                                                                                                                     aTGTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTGGGATGGATTATGTTAATTCAAGACCTAAAACAGATATGCCGGCACAGaaa                                                                                                       >  1:265315/1‑168 (MQ=255)                                                                                                                                                                                                       gTAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTGGGATGGATTATGTTAATTCAAGACCTAAAACAGATATGCCGGCACAGAAAAGTGCGGTCGTTTTTTTc                                                                                     <  1:210463/184‑1 (MQ=255)                                                                                                                                                                                                        tAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTGGGATGGATTATGTTAATTCAAGACCTAAAACAGATATGCCGGCACAGAAAAGTGCGGTCGTTTTTTTCAGTGTTTTTTATTATTGAAACGGCTGTCAGTATAACCGAAATTGATTGGTGTAAGGCaaaataaaat                  <  1:89245/250‑1 (MQ=255)                                                                                                                                                                                                        tAAGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTGGGATGGATTATGTTAATTCAAGACCTAAAACAGATATGCCGGCACAGAAAAGTGCGGTCGTTTTTTTCAGTGTTTTTTATTATTGAAACGGCTGTCAGTATAACCGAAATTGATTGGTGTAAGGCAAAATAAAAtt                 >  1:24819/1‑251 (MQ=255)                                                                                                                                                                                                          aGGAAACACGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTGgga                                                                                                                                                      <  1:528577/116‑1 (MQ=255)                                                                                                                                                                                                               acacGACAACATCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTGGGATGGATTATGTTAATTCAAGACCTAAAACAGATATGCCGGCACAGAAAAGTGCGGTCGTTTTTTTCAGTGTTTTTTATTATTGAAACGGCTGTCAGTATAACCGAAATTGATTGGTGTAAGGCAAAATAAAATTCATTAAg          >  1:98664/1‑251 (MQ=255)                                                                                                                                                                                                                  cGACAACATCGCGCAATGGCAATACCGGTATGGGTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTGgga                                                                                                                                                      >  1:350775/1‑108 (MQ=255)                                                                                                                                                                                                                  cGACAACATCGCGCAATGGCAATACCGGCATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTGgga                                                                                                                                                      >  1:751324/1‑108 (MQ=255)                                                                                                                                                                                                                       acaTCGCGCAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTGGGATGGATTATGTTAATTCAAGACCTAAAACAGATATGCCGGCACAGAAAAGTGCGGTCGTTTTTTTCAGTGTTTTTTATTATTGAAACGGCTGTCAGTATAACCGAAATTGATTGGTGTAAGGCAAAATAAAATTCATTAAGAATGGCgg  <  1:861430/251‑1 (MQ=255)                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                   CACTTTTACTGTGCCGTCCGGCAACTTTAGCAGCTGAATAATGTTGGCAATAGTGCCCACATCATATAAATCTTCAATGCCCGGCTCTTCCAATTCCGCCTGTTTTTGCGAAACCAACAACAGCTGTTTTTCATTATTCATGGCTTCGTCAAGACTGCTAATGGATCGCGGTCTGCCGACAAATAACGGCATCACCATGTAAGGAAACACGACAACATCGCGTAATGGCAATACCGGTATGGTTTGTAATTCTGATTTTTTTGCTCTCATCATGTACTCTCTTATCTAAATTCCGCTGGCTTGGCTCTATATTGGGATGGATTATGTTAATTCAAGACCTAAAACAGATATGCCGGCACAGAAAAGTGCGGTCGTTTTTTTCAGTGTTTTTTATTATTGAAACGGCTGTCAGTATAACCGAAATTGATTGGTGTAAGGCAAAATAAAATTCATTAAGAATGGCGG  >  1/1059005‑1059469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑