Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,058,310	A→G	intergenic (+17387/+1154)	unknown → / ← unknown	tRNA‑Trp‑CCA/tRNA‑Ser‑GGA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,058,310	0	A	G	100.0%	212.4 / NA	78	intergenic (+17387/+1154)	unknown/unknown	tRNA‑Trp‑CCA/tRNA‑Ser‑GGA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (43/35);  total (43/35)

GCAGTGATCCAATGTAAGTTTTACGGTGACCGCGAATTTCCGCTTCGTCACGCACGCCCCCCAGCGCCATACGCACATATTTACGCCCTGTTGCATTGGCGATAGACTGTCCTAAAGAAGTTTTACCTACCCCCGGTGGTCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGACATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCCAATCAATATAACTGCGCACCACCGTTGCTTCCGCCGACATCGGCGACATCATTTTTAATTTTTGTAATTCGGCTTCGGTTTTCTCGTGCGCTTCCAACGGCATTTTCGCCTGTTCAATTTTTTGGCGCAGGTGCTCAATTTCACTCACGGTATTTTCTACTT  >  1/1058068‑1058547                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                              gCAGTGATCCAATGTAAGTTTTACGGTGACCGCGAATTTCCGCTTCGTCACGCACGCCCCCCAGCGCCATACGCACATATTTACGCCCTGTTGCATTGGCGATAGACTGTCCTAAAGAAGTTTTACCTACCCCCGGTGGTCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTaaa                                                                                                                                                                                                                                       >  1:466578/1‑251 (MQ=255)     tGATCCAATGTAAGTTTTACGGTGACCGCGAATTTCCGCTTCGTCACGCACGCCCCCCAGCGCCATACGCACATATTTACGCCCTGTTGCATTGGCGATAGACTGTCCTAAAGAAGTTTTACCTACCCCCGGTGGTCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAAt                                                                                                                                                                                                                                     <  1:302/249‑1 (MQ=255)      gATCCAATGTAAGTTTTACGGTGACCGCGAATTTCCGCTTCGTCACGCACGCCCCCCAGCGCCATACGCACATATTTACGCCCTGTTGCATTGGCGATAGACTGTCCTAAAGAAGTTTTACCTACCCCCGGTGGTCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAAttt                                                                                                                                                                                                                                   >  1:509024/1‑250 (MQ=255)                  gTTTTACGGTGACCGCGAATTTCCGCTTCGTCACGCACGCCCCCCAGCGCCATACGCACATATTTACGCCCTGTTGCATTGGCGATAGACTGTCCTAAAGAAGTTTTACCTACCCCCGGTGGTCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTgg                                                                                                                                                                                                                       >  1:558548/1‑250 (MQ=255)                                         cGCTTCGTCACGCACGCCCCCCAGCGCCATACGCACATATTTACGCCCTGTTGCATTGGCGATAGACTGTCCTAAAGAAGTTTTACCTACCCCCGGTGGTCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTtactac                                                                                                                                                                                               <  1:391256/251‑1 (MQ=255)                                             tCGTCACGCACGCCCCCCAGCGCCATACGCACATATTTACGCCCTGTTGCATTGGCGATAGACTGTCCTAAAGAAGTTTTACCTACCCCCGGTGGTCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGttt                                                                                                                                                                                           >  1:131361/1‑251 (MQ=255)                                                 cacgcacgCCCCCCAGCGCCATACGCACATATTTACGCCCTGTTGCATTGGCGATAGACTGTCCTAAAGAAGTTTTACCTACCCCCGGTGGTCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGc                                                                                                                                                                                       <  1:590554/251‑1 (MQ=255)                                                  acgcacgcCCCCCAGCGCCATACGCACATATTTACGCCCTGTTGCATTGGCGATAGACTGTCCTAAAGAAGTTTTACCTACCCCCGGTGGTCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGcc                                                                                                                                                                                      <  1:800948/251‑1 (MQ=255)                                                           ccccAGCGCCATACGCACATATTTACGCCCTGTTGCATTGGCGATAGACTGTCCTAAAGAAGTTTTACCTACCCCCGGTGGTCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACtt                                                                                                                                                                             >  1:289962/1‑251 (MQ=255)                                                                gcgcCATACGCACATATTTACGCCCTGTTGCATTGGCGATAGACTGTCCTAAAGAAGTTTTACCTACCCCCGGTGGTCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTaa                                                                                                                                                                         >  1:54033/1‑250 (MQ=255)                                                                 cgcCATACGCACATATTTACGCCCTGTTGCATTGGCGATAGACTGTCCTAAAGAAGTTTTACCTACCCCCGGTGGTCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACa                                                                                                                                                                       <  1:626804/251‑1 (MQ=255)                                                                 cgcCATACGCACATATTTACGCCCTGTTGCATTGGCGATAGACTGTCCTAAAGAAGTTTTACCTACCCCCGGTGGTCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACa                                                                                                                                                                       <  1:683212/251‑1 (MQ=255)                                                                   ccATACGCACATATTTACGCCCTGTTGCATTGGCGATAGACTGTCCTAAAGAAGTTTTACCTACCCCCGGTGGTCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACAt                                                                                                                                                                      <  1:582301/250‑1 (MQ=255)                                                                   ccATACGCACATATTTACGCCCTGTTGCATTGGCGATAGACTGTCCTAAAGAAGTTTTACCTACCCCCGGTGGTCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATc                                                                                                                                                                     <  1:243338/251‑1 (MQ=255)                                                                        cGCACATATTTACGCCCTGTTGCATTGGCGATAGACTGTCCTAAAGAAGTTTTACCTACCCCCGGTGGTCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTc                                                                                                                                                                                                         >  1:794788/1‑210 (MQ=255)                                                                        cGCACATATTTACGCCCTGTTGCATTGGCGATAGACTGTCCTAAAGAAGTTTTACCTACCCCCGGTGGTCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCcaat                                                                                                                                                                 >  1:853512/1‑250 (MQ=255)                                                                        cGCACATATTTACGCCCTGTTGCATTGGCGATAGACTGTCCTAAAGAAGTTTTACCTACCCCCGGTGGTCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGCCACTTGTAACATCcaat                                                                                                                                                                 >  1:712143/1‑250 (MQ=255)                                                                          cacaTATTTACGCCCTGTTGCATTGGCGATAGACTGTCCTAAAGAAGTTTTACCTACCCCCGGTGGTCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGt                                                                                                                                                                                             >  1:630089/1‑220 (MQ=255)                                                                          cacaTATTTACGCCCTGTTGCATTGGCGATAGACTGTCCTAAAGAAGTTTTACCTACCCCCGGTGGTCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCcaatcaa                                                                                                                                                              >  1:162835/1‑251 (MQ=255)                                                                          cacaTATTTACGCCCTGTTGCATTGGCGATAGACTGTCCTAAAGAAGTTTTACCTACCCCCGGTGGTCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTCTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCcaatcaa                                                                                                                                                              >  1:85343/1‑251 (MQ=255)                                                                              tatTTACGCCCTGTTGCATTGGCGATAGACTGTCCTAAAGAAGTTTTACCTACCCCCGGTGGTCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTtactac                                                                                                                                                                                               <  1:347618/214‑1 (MQ=255)                                                                              tatTTACGCCCTGTTGCATTGGCGATAGACTGTCCTAAAGAAGTTTTACCTACCCCCGGTGGTCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTtacta                                                                                                                                                                                                <  1:549318/213‑1 (MQ=255)                                                                              tatTTACGCCCTGTTGCATTGGCGATAGACTGTCCTAAAGAAGTTTTACCTACCCCCGGTGGTCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGt                                                                                                                                                                                             <  1:804382/216‑1 (MQ=255)                                                                              tatTTACGCCCTGTTGCATTGGCGATAGACTGTCCTAAAGAAGTTTTACCTACCCCCGGTGGTCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTa                                                                                                                                                                          <  1:546354/235‑1 (MQ=255)                                                                               atTTACGCCCTGTTGCATTGGCGATAGACTGTCCTAAAGAAGTTTTACCTACCCCCGGTGGTCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCCAATCAATATaa                                                                                                                                                         >  1:110551/1‑251 (MQ=255)                                                                                tttACGCCCTGTTGCATTGGCGATAGACTGTCCTAAAGAAGTTTTACCTACCCCCGGTGGTCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCCAATCAATATAAc                                                                                                                                                        <  1:559893/251‑1 (MQ=255)                                                                                tttACGCCCTGTTGCATTGGCGATAGACTGTCCTAAAGAAGTTTTACCTACCCCCGGTGGTCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCCAATCAATATAAc                                                                                                                                                        <  1:375390/251‑1 (MQ=255)                                                                                tttACGCCCTGTTGCATTGGCGATAGACTGTCCTAAAGAAGTTTTACCTACCCCCGGTGGTCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCCAATCAATATAAc                                                                                                                                                        <  1:448173/251‑1 (MQ=255)                                                                                  tACGCCCTGTTGCATTGGCGATAGACTGTCCTAAAGAAGTTTTACCTACCCCCGGTGGTCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCCAATCAATATAACTg                                                                                                                                                      <  1:627517/251‑1 (MQ=255)                                                                                        cTGTTGCATTGGCGATAGACTGTCCTAAAGAAGTTTTACCTACCCCCGGTGGTCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTtactac                                                                                                                                                                                               >  1:387909/1‑204 (MQ=255)                                                                                        cTGTTGCATTGGCGATAGACTGTCCTAAAGAAGTTTTACCTACCCCCGGTGGTCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAAc                                                                                                                                                                        <  1:542470/227‑1 (MQ=255)                                                                                          gTTGCATTGGCGATAGACTGTCCTAAAGAAGTTTTACCTACCCCCGGTGGTCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCCAATCAATATAACTGCGcacca                                                                                                                                               >  1:561874/1‑250 (MQ=255)                                                                                           ttGCATTGGCGATAGACTGTCCTAAAGAAGTTTTACCTACCCCCGGTGGTCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCCAATCAATATAACTGCGcaccac                                                                                                                                              <  1:562090/250‑1 (MQ=255)                                                                                                  ggCGATAGACTGTCCTAAAGAAGTTTTACCTACCCCCGGTGGTCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCTGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCCAATCAATATAACTGCGCACCACCGTTGCtt                                                                                                                                      >  1:15665/1‑251 (MQ=255)                                                                                                  ggCGATAGACTGTCCTAAAGAAGTTTTACCTACCCCCGGTGGTCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCCAATCAATATAACTg                                                                                                                                                      >  1:833058/1‑235 (MQ=255)                                                                                                  ggCGATAGACTGTCCTAAAGAAGTTTTACCTACCCCCGGTGGTCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCCAATCAATATAACTGCGCACCACCGTTGCtt                                                                                                                                      >  1:563197/1‑251 (MQ=255)                                                                                                  ggCGATAGACTGTCCTAAAGAAGTTTTACCTACCCCCGGTGGTCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCCAATCAATATAACTGCGCACCACCGTTGCtt                                                                                                                                      >  1:171147/1‑251 (MQ=255)                                                                                                  ggCGATAGACTGTCCTAAAGAAGTTTTACCTACCCCCGGTGGTCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCCAATCAATATAACTGCGCACCACCGTTGCtt                                                                                                                                      >  1:754895/1‑251 (MQ=255)                                                                                                   gCGATAGACTGTCCTAAAGAAGTTTTACCTACCCCCGGTGGTCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATcc                                                                                                                                                                    <  1:249652/220‑1 (MQ=255)                                                                                                   gCGATAGACTGTCCTAAAGAAGTTTTACCTACCCCCGGTGGTCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCCAATCAATATAACTGCGCACCACCGTTGCtt                                                                                                                                      >  1:819724/1‑250 (MQ=255)                                                                                                   gCGATAGACTGTCCTAAAGAAGTTTTACCTACCCCCGGTGGTCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCCAATCAATATAACTGCGCACCACCGTTGCtt                                                                                                                                      <  1:547139/250‑1 (MQ=255)                                                                                                             gTCCTAAAGAAGTTTTACCTACCCCCGGTGGTCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCCAATCAATATAAc                                                                                                                                                        <  1:44314/222‑1 (MQ=255)                                                                                                              tCCTAAAGAAGTTTTACCTACCCCCGGTGGTCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCCAATCAATATAACTGCGCACCACCGTTGCTTCCGCCGACATCg                                                                                                                          >  1:303602/1‑251 (MQ=255)                                                                                                                  aaaGAAGTTTTACCTACCCCCGGTGGTCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACa                                                                                                                                                                       >  1:886432/1‑202 (MQ=255)                                                                                                                      aagTTTTACCTACCCCCGGTGGTCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCCAATCAATATAACTGCGCACCACCGTTGCTTCCGCCGACATCGGCGAcatc                                                                                                                  <  1:696827/251‑1 (MQ=255)                                                                                                                        gTTTTACCTACCCCCGGTGGTCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCCCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCCAATCAATATAACTGCGCACCACCGTTGCTTCCGCCGACATCGGCGAcatcat                                                                                                                >  1:326815/1‑251 (MQ=255)                                                                                                                                 accCCCGGTGGTCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCCAATCAATATAACTGCGCACCACCGTTGCTTCCGCCGACATCGGCGACATCATTTTTAAttt                                                                                                       >  1:706891/1‑251 (MQ=255)                                                                                                                                     ccGGTGGTCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCCAATCAATATAACTGCGCACCACCGTTGCTTCCGCCGACATCGGCGACATCATTTTTAATTTTTGt                                                                                                   >  1:94044/1‑251 (MQ=255)                                                                                                                                        gtggtCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCCAATCAATATAACTGCGCACCACCg                                                                                                                                            <  1:879086/207‑1 (MQ=255)                                                                                                                                          ggtCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCCAATCAATATAACTGCGCACCACCGTTGCTTCCGCCGACATCGGCGACATCATTTTTAATTTTTGTAAtt                                                                                               >  1:332103/1‑250 (MQ=255)                                                                                                                                                     acaaaGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGAGCGGCATCTAAAAt                                                                                                                                                                                                                                     <  1:133381/105‑1 (MQ=255)                                                                                                                                                                            gATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCCAATCAatat                                                                                                                                                           <  1:64369/156‑1 (MQ=255)                                                                                                                                                                                  aaCGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCCAATCAATATAACTGCGCACCACCGTTGCTTCCGCCGACATCGGCGACATCATTTTTAATTTTTGTAATTCGGCTTCGGTTTTCTCGTGCGCTTCCAACGGCATTTTCGcc                                                      <  1:56904/251‑1 (MQ=255)                                                                                                                                                                                      ggTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCCAATCAATATAACTGCGCACCACCGTTGCTTCCGCCGACATCGGCGACATCATTTTTAATTTTTGTAATTCGGCTTCGGTTTtct                                                                                >  1:537780/1‑221 (MQ=255)                                                                                                                                                                                      ggTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCCAATCAATATAACTGCGCACCACCGTTGCTTCCGCCGACATCGGCGACATCATTTTTAATTTTTGTAATTCGGCTTCGGTTTTCTCGTGCGCTTCCAACGGCATTTTCGCCTGtt                                                  >  1:525990/1‑251 (MQ=255)                                                                                                                                                                                            cACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCCAATCAATATAACTGCGCACCACCGTTGCTTCCGCCGACATCGGCGACATCATTTTTAATTTTTGTAATTCGGCTTCGGTTTTCTCGTGCGCTTCCAACGGCATTTTCGCCTGTTCAAttt                                            <  1:856539/251‑1 (MQ=255)                                                                                                                                                                                              ccGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCCAATCAATATAACTGCGCACCACCGTTGCTTCCGCCGACATCGGCGACATCATTTTTAATTTTTGTAATTCGGCTTCGGTTTTCTCGTGCGCTTCCAACGGCATTTTCGCCTGTTCAAttttt                                          >  1:800519/1‑251 (MQ=255)                                                                                                                                                                                                gCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCCAATCAATATAACTGCGCACCACCGTTGCTTCCGCCGACATCGGCGACATCATTTTTAATTTTTGTAATTCGGCTTCGGTTTTCTCGTGCGCTTCCAACGGCATTTTCGCCTGTTCAATTTTTTg                                        >  1:529982/1‑251 (MQ=255)                                                                                                                                                                                                   aGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTaa                                                                                                                                                                         >  1:486761/1‑119 (MQ=255)                                                                                                                                                                                                   aGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCCAATCAATATAACTGCGCACCACCGTTGCTTCCGCCGACATCGGCGACATCATTTTTAATTTTTGTAATTCGGCTTCGGTTTTCTCGTGCGCTTCCAACGGCATTTTCGcc                                                      >  1:368277/1‑234 (MQ=255)                                                                                                                                                                                                     gTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCCAATCAATATAACTGCGCACCACCGTTGCTTCCGCCGACATCGGCGACATCATTTTTAATTTTTGTAATTCGGCTTCGGTTTTCTCGTGCGCTTCCAACGGCATTTTCGCCTGTTCAATTTTTTGGCGCa                                   >  1:561015/1‑251 (MQ=255)                                                                                                                                                                                                     gTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCCAATCAATATAACTGCGCACCACCGTTGCTTCCGCCGACATCGGCGACATCATTTTTAATTTTTGTAATTCGGCTTCGGTTTTCTCGTGCGCTTCCAACGGCATTTTCGCCTGTTCAATTTTTTGGCGCa                                   >  1:507716/1‑251 (MQ=255)                                                                                                                                                                                                        cTCCAGAATACGTTCTTTCACCCGCCCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCCAATCAATATAACTGCGCACCACCGTTGCTTCCGCCGACATCGGCGACATCATTTTTAATTTTTGTAATTCGGCTTCGGTTTTCTCGTGCGCTTCCAACGGCATTTTCGCCTGTTCAATTTTTTGGCGCAGGt                                >  1:291837/1‑251 (MQ=255)                                                                                                                                                                                                          ccAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCCAATCAATATAACTGCGCACCACCGTTGCTTCCGCCGACATCGGCGACATCATTTTTAATTTTTGTAATTCGGCTTCGGTTTTCTCGTGCGCTTCCAACGGCATTTTCGcc                                                      >  1:494957/1‑227 (MQ=255)                                                                                                                                                                                                          ccAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCCAATCAATATAACTGCGCACCACCGTTGCTTCCGCCGACATCGGCGACATCATTTTTAATTTTTGTAATTCGGCTTCGGTTTTCTCGTGCGCTTCCAACGGCATTTTCGCCTGTTCAATTTTTTGGCGCAGGTg                               >  1:399328/1‑250 (MQ=255)                                                                                                                                                                                                             gAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCCAATCAATATAACTGCGCACCACCGTTGCTTCCGCCGACATCGGCGACATCATTTTTAATTTTTGTAATTCGGCTTCGGTTTTCTCGTGCGCTTCCAACGGCATTTTCg                                                        <  1:740377/222‑1 (MQ=255)                                                                                                                                                                                                             gAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCCAATCAATATAACTGCGCACCACCGTTGCTTCCGCCGACATCGGCGACATCATTTTTAATTTTTGTAATTCGGCTTCGGTTTTCTCGTGCGCTTCCAACGGCATTTTCGCCTGTTCAATTTTTTGGCGCAGGTGCTCa                           >  1:114361/1‑251 (MQ=255)                                                                                                                                                                                                              aaTACGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCCAATCAATATAACTGCGCACCACCGTTGCTTCCGCCGACATCGGCGACATCATTTTTAATTTTTGTAATTCGGCTTCGGTTTTCTCGTGCGCTTCCAACGGCATTTTCGCCTGTTCAATTTTTTGGCGCAGGTGCTCaa                          <  1:718338/251‑1 (MQ=255)                                                                                                                                                                                                                 aCGTTCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCCAATCAATATAACTGCGCACCACCGTTGCTTCCGCCGACATCGGCGACATCATTTTTAATTTTTGTAATTCGGCTTCGGTTTTCTCGTGCGCTTCCAACGGCATTTTCGCCTGTTCAATTTTTTGGCGCAGGTGCTCAAttt                       <  1:459534/251‑1 (MQ=255)                                                                                                                                                                                                                    ttCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCCAATCAATATAACTGCGCACCACCGTTGCTTCCGCCGACATCGGCGACATCATTTTTAATTTTTGTAATTCGGCTTCGGTTTTCTCGTGCGCTTCCAACGGCATTTTCGCCTGTTCAATTTTTTGGCGCAGGTGCTCAATTtcac                    <  1:699549/251‑1 (MQ=255)                                                                                                                                                                                                                     tCTTTCACCCGCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCCAATCAATATAACTGCGCACCACCGTTGCTTCCGCCGACATCGGCGACATCATTTTTAATTTTTGTAATTCGGCTTCGGTTTTCTCGTGCGCTTCCAACGGCATTTTCGCCTGTTCAATTTTTTGGCGCAGGTGCTCAATTtca                     >  1:305843/1‑249 (MQ=255)                                                                                                                                                                                                                               gCTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCCAATCAATATAACTGCGCACCACCGTTGCTTCCGCCGACATCGGCGACATCATTTTTAATTTTTGTAATTCGGCTTCGGTTTTCTCGTGCGCTTCCAACGGCATTTTCGcc                                                      <  1:347314/206‑1 (MQ=255)                                                                                                                                                                                                                                cTCCAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCCAATCAATATAACTGCGCACCACCGTTGCTTCCGCCGACATCGGCGACATCATTTTTAATTTTTGTAATTCGGCTTCGGTTTTCTCGTGCGCTTCCAACGGCATTTTCGCCTGTTCAATTTTTTGGCGCAGGTGCTCAATTTCACTCACGGTAtttt        >  1:632998/1‑251 (MQ=255)                                                                                                                                                                                                                                  ccAAACCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCCAATCAATATAACTGCGCACCACCGTTGCTTCCGCCGACATCGGCGACATCATTTTTAATTTTTGTAATTCGGCTTCGGTTTTCTCGTGCGCTTCCAACGGCATTTTCGCCTGTTCAATTTTTTGGCGCAGGTGCTCAATTTCACTCACGGTATTTTCt      <  1:571896/251‑1 (MQ=255)                                                                                                                                                                                                                                    aaaCCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCCAATCAATATAACTGCGCACCACCGTTGCTTCCGCCGACATCGGCGACATCATTTTTAATTTTTGTAATTCGGCTTCGGTTTTCTCGTGCGCTTCCAACGGCATTTTCGCCTGTTCAATTTTTTGGCGCAGGTGCTCAATTTCACTCACGGTATTTTCTAc    <  1:744718/251‑1 (MQ=255)                                                                                                                                                                                                                                    aaaCCGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCCAATCAATATAACTGCGCACCACCGTTGCTTCCGCCGACATCGGCGACATCATTTTTAATTTTTGTAATTCGGCTTCGGTTTTCTCGTGCGCTTCCAACGGCATTTTCGCCTGTTCAATTTTTTGGCGCAGGTGCTCAATTTCACTCACGGTATTTTCTAc    <  1:700826/251‑1 (MQ=255)                                                                                                                                                                                                                                       ccGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCCAATCAATATAACTGCGCACCACCGTTGCTTCCGCCGACATCGGCGACATCATTTTTAATTTTTGTAATTCGGCTTCGGTTTTCTCGTGCGCTTCCAACGGCATTTTCGCCTGTTCAATTTTTTGGCGCAGGTGCTCAAttt                       >  1:849406/1‑229 (MQ=255)                                                                                                                                                                                                                                       ccGTAATGATCGGCATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCCAATCAATATAACTGCGCACCACCGTTGCTTCCGCCGACATCGGCGACATCATTTTTAATTTTTGTAATTCGGCTTCGGTTTTCTCGTGCGCTTCCAACGGCATTTTCGCCTGTTCAATTTTTTGGCGCAGGTGCTCAATTTCACTCACGGTATTTTCTACtt  >  1:591065/1‑250 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                              GCAGTGATCCAATGTAAGTTTTACGGTGACCGCGAATTTCCGCTTCGTCACGCACGCCCCCCAGCGCCATACGCACATATTTACGCCCTGTTGCATTGGCGATAGACTGTCCTAAAGAAGTTTTACCTACCCCCGGTGGTCCCACCAAACAAAGAATCGGGCCTTTCAACTGATTTAAACGGGTTTGCACCGCAAGGTACTCCAGAATACGTTCTTTCACCCGCTCCAAACCGTAATGATCGACATCTAAAATTTCCTGCGCTTTGGCAAGATCTTTTTTCACTTTACTACGTTTGTGCCAAGGCACTTGTAACATCCAATCAATATAACTGCGCACCACCGTTGCTTCCGCCGACATCGGCGACATCATTTTTAATTTTTGTAATTCGGCTTCGGTTTTCTCGTGCGCTTCCAACGGCATTTTCGCCTGTTCAATTTTTTGGCGCAGGTGCTCAATTTCACTCACGGTATTTTCTACTT  >  1/1058068‑1058547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑