Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,040,937	A→G	intergenic (+14/+18527)	unknown → / ← unknown	tRNA‑Trp‑CCA/tRNA‑Ser‑GGA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,040,937	0	A	G	100.0%	217.1 / NA	75	intergenic (+14/+18527)	unknown/unknown	tRNA‑Trp‑CCA/tRNA‑Ser‑GGA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/40);  total (35/40)

CCTATGTGAGAGTAGGTCACTGCTAATTCTCATTCTGGAGTGGTAGTTCAGCTGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCATTCCGCCAACTTTTGTAACCTTTTCAGGTTATACATACCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTAAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTGAGCTCTATATTGCACAGGGTTTAAATCGTCTAACCCGAGCTTAATTTGCTCCTAGGTATAATAACGAATATATCATGTATCATCCATTCTGATTCATCAATGTCCTTAAACTGA  >  1/1040701‑1041170                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                          ccTATGTGAGAGTAGGTCACTGCTAATTCTCATTCTGGAGTGGTAGTTCAGCTGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCATTCCGCCAACTTTTGTAACCTTTTCAGGTTATACATACCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGt                                                                                                                                                                                                                              >  1:412654/1‑250 (MQ=255)  cTATGTGAGAGTAGGTCACTGCTAATTCTCATTCTGGAGTGGTAGTTCAGCTGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCATTCCGCCAACTTTTGTAACCTTTTCAGGTTATACATACCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGt                                                                                                                                                                                                                              >  1:391733/1‑249 (MQ=255)       tgAGAGTAGGTCACTGCTAATTCTCATTCTGGAGTGGTAGTTCAGCTGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCATTCCGCCAACTTTTGTAACCTTTTCAGGTTATACATACCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATc                                                                                                                                                                                                                       <  1:427054/251‑1 (MQ=255)       tgAGAGTAGGTCACTGCTAATTCTCATTCTGGAGTGGTAGTTCAGCTGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCATTCCGCCAACTTTTGTAACCTTTTCAGGTTATACATACCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATc                                                                                                                                                                                                                       <  1:106134/251‑1 (MQ=255)        gagaGTAGGTCACTGCTAATTCTCATTCTGGAGTGGTAGTTCAGCTGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCATTCCGCCAACTTTTGTAACCTTTTCAGGTTATACATACCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGtct                                                                                                                                                                                                                            <  1:659346/245‑1 (MQ=255)            gTAGGTCACTGCTAATTCTCATTCTGGAGTGGTAGTTCAGCTGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCATTCCGCCAACTTTTGTAACCTTTTCAGGTTATACATACCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGttt                                                                                                                                                                                                                  >  1:809954/1‑251 (MQ=255)                                         tGGTAGTTCAGCTGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCATTCCGCCAACTTTTGTAACCTTTTCAGGTTATACATACCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAAc                                                                                                                                                                                      <  1:650979/250‑1 (MQ=255)                                           gTAGTTCAGCTGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCATTCCGCCAACTTTTGTAACCTTTTCAGGTTATACATACCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGaaaa                                                                                                                                                                                       >  1:162268/1‑247 (MQ=255)                                            tAGTTCAGCTGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCATTCCGCCAACTTTTGTAATCTTTTCAGGTTATACATACCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGt                                                                                                                                                                                   <  1:443007/250‑1 (MQ=255)                                                    cTGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCATTCCGCCAACTTTTGTAACCTTTTCAGGTTATACATACCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTaa                                                                                                                                                                            <  1:521414/249‑1 (MQ=255)                                                      ggTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCATTCCGCCAACTTTTGTAACCTTTTCAGGTTATACATACCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAAt                                                                                                                                                                         >  1:417240/1‑250 (MQ=255)                                                      ggTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCATTCCGCCAACTTTTGTAACCTTTTCAGGTTATACATACCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAAt                                                                                                                                                                         >  1:822897/1‑250 (MQ=255)                                                       gTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCATTCCGCCAACTTTTGTAACCTTTTCAGGTTATACATACCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCa                                                                                                                                                                       >  1:478370/1‑251 (MQ=255)                                                        ttAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCATTCCGCCAACTTTTGTAACCTTTTCAGGTTATACATACCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGCTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAg                                                                                                                                                                      >  1:379646/1‑251 (MQ=255)                                                           gAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCATTCCGCCAACTTTTGTAACCTTTTCAGGTTATACATACCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGAtt                                                                                                                                                                   >  1:833322/1‑251 (MQ=255)                                                                        tCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCATTCCGCCAACTTTTGTAACCTTTTCAGGTTATACATACCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAgcgactt                                                                                                                                                      >  1:14663/1‑245 (MQ=255)                                                                          aCGCAGGGGGTCGCGGGTTCGAGTCCCGTCCATTCCGCCAACTTTTGTAACCTTTTCAGGTTATACATACCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGa                                                                                                                                                    >  1:719626/1‑251 (MQ=255)                                                                                           ttCGAGTCCCGTCCATTCCGCCAACTTTTGTAACCTTTTCAGGTTATACATACCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGAc                                                                                                                                    <  1:338181/250‑1 (MQ=255)                                                                                                 tCCCGTCCATTCCGCCAACTTTTGTAACCTTTTCAGGTTATACATACCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAAc                                                                                                                             <  1:820363/251‑1 (MQ=255)                                                                                                  cccGTCCATTCCGCCAACTTTTGTAACCTTTTCAGGTTATACATACCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTCACAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATaa                                                                                                                              >  1:126399/1‑249 (MQ=255)                                                                                                        cATTCCGCCAACTTTTGTAACCTTTTCAGGTTATACATACCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAatat                                                                                                                      <  1:311283/251‑1 (MQ=255)                                                                                                        cATTCCGCCAACTTTTGTAACCTTTTCAGGTTATACATACCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAatat                                                                                                                      >  1:276823/1‑251 (MQ=255)                                                                                                                    ttttGTAACCTTTTCAGGTTATACATACCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTGAGCTCtatac                                                                                                          <  1:358485/251‑2 (MQ=255)                                                                                                                    ttttGTAACCTTTTCAGGTTATACATACCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTGAGCTCtatac                                                                                                          <  1:320011/251‑2 (MQ=255)                                                                                                                    ttttGTAACCTTGTCAGGTTATACATACCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTGAGCTCtatac                                                                                                          <  1:705750/251‑2 (MQ=255)                                                                                                                     tttGTAACCTTTTCAGGTTATACATACCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTg                                                                                                                    >  1:649439/1‑240 (MQ=255)                                                                                                                       tGTAACCTTTTCAGGTTATACATACCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTGAGCTCTATACTGc                                                                                                       <  1:379587/251‑1 (MQ=255)                                                                                                                       tGTAACCTTTTCAGGTTATACATACCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTGAGCTCTATACTGc                                                                                                       <  1:160146/251‑1 (MQ=255)                                                                                                                       tGTAACCTTTTCAGGTTATACATACCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTGAGCTCTATACTGc                                                                                                       <  1:607408/251‑1 (MQ=255)                                                                                                                         tAACCTTTTCAGGTTATACATACCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTGAGCTCTATACTGcac                                                                                                     <  1:895590/251‑1 (MQ=255)                                                                                                                            ccTTTTCAGGTTATACATACCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAatat                                                                                                                      <  1:217707/231‑1 (MQ=255)                                                                                                                              ttttCAGGTTATACATACCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTGAGCTCtatac                                                                                                          >  1:835972/1‑240 (MQ=255)                                                                                                                              ttttCAGGTTATACATACCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATCTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTGAGCTCTATACTGCACAGGGt                                                                                                <  1:881432/251‑1 (MQ=255)                                                                                                                               tttCAGGTTATACATACCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTGAGCTCtatac                                                                                                          <  1:507644/240‑2 (MQ=255)                                                                                                                                   aGGTTATACATACCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTGAGCTCTATACTGcac                                                                                                     >  1:32587/1‑241 (MQ=255)                                                                                                                                    ggTTATACATACCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCGTCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTGAGCTCTATACTGCACAGGGTTTAAAt                                                                                          >  1:413248/1‑251 (MQ=255)                                                                                                                                    ggTTATACATACCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAACATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTGAGc                                                                                                                 >  1:424508/1‑228 (MQ=255)                                                                                                                                    ggTTATACATACCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTGAGCTCTATACTGCACAg                                                                                                   >  1:465185/1‑242 (MQ=255)                                                                                                                                     gTTATACATACCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTTCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTGAGCTCTATACTGCACAGGGTTTaaa                                                                                           <  1:456053/249‑1 (MQ=255)                                                                                                                                     gTTATACATACCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTGAGCTCTATACTGCACAGGGTTTAAAt                                                                                          >  1:688894/1‑250 (MQ=255)                                                                                                                                     gTTATACATACCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTGAGCTCTATACTGCACAGGGTTTAAAt                                                                                          <  1:882174/250‑1 (MQ=255)                                                                                                                                     gTTATACATACCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTGAGCTCTATACTGCACAGGGTTTAAAt                                                                                          <  1:494588/250‑1 (MQ=255)                                                                                                                                     gTTATACATACCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTGAGCTCTATACTGCACAGGGTTTAAAt                                                                                          <  1:607186/250‑1 (MQ=255)                                                                                                                                       tataCATACCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTGAGCTCTATACTGc                                                                                                       >  1:45842/1‑235 (MQ=255)                                                                                                                                        atacatacCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTGAGCTCTATACTGc                                                                                                       <  1:114644/234‑1 (MQ=255)                                                                                                                                        atacatacCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTGAGCTCTATACTGCACAGGGTTTAAAt                                                                                          >  1:643098/1‑247 (MQ=255)                                                                                                                                           catacCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATc                                                                                                                                                                                                                       <  1:650598/119‑1 (MQ=255)                                                                                                                                             tacCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTGAGCTCTATACTGcac                                                                                                     >  1:735932/1‑231 (MQ=255)                                                                                                                                             tacCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTGAGCTCTATACTGcac                                                                                                     >  1:547344/1‑231 (MQ=255)                                                                                                                                             tacCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTGAGCTCTATACTGc                                                                                                       >  1:732301/1‑229 (MQ=255)                                                                                                                                             tacCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTGAGCTCTATACTGCACAGGGTTTAAATCGTCTAAc                                                                                  >  1:631181/1‑250 (MQ=255)                                                                                                                                             tacCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTGAGCTCTATACTGCACAGGGTTTAAATCGTCTAAc                                                                                  >  1:821521/1‑250 (MQ=255)                                                                                                                                                       ggCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTGAGCTCTATACTGCACAGGGTTTAAATCGTCTAACCCGAGCTTAAt                                                                       >  1:688874/1‑251 (MQ=255)                                                                                                                                                             gTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTGAGCTCTATACTGCACAGGGTTTAAATCGTCTAACCCGAGCTTAATTTGCt                                                                  >  1:132504/1‑250 (MQ=255)                                                                                                                                                                 aaTTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTGAGCTCTATACTGCACAGGGTTTAAATCGTCTAACCCGAGCTTAATTTGCt                                                                  <  1:280074/246‑1 (MQ=255)                                                                                                                                                                    tGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTGAGCTCtatac                                                                                                          >  1:692549/1‑202 (MQ=255)                                                                                                                                                                     ggTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAAc                                                                                                                                                                                                                                        <  1:4405/76‑1 (MQ=255)                                                                                                                                                                      gTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGt                                                                                                                                                                                                                              >  1:477242/1‑85 (MQ=255)                                                                                                                                                                      gTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTGAGCTCTATACTGc                                                                                                       <  1:173794/204‑1 (MQ=255)                                                                                                                                                                          agCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTGAGCTCTATACTGCACAGGGTTTAAATCGTCTAACCCGAGCTTAATTTGCTCCTAGGTataat                                                      <  1:348031/249‑1 (MQ=255)                                                                                                                                                                            cACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTGAGCTCTATACTGCACAGGGTTTAAATCGTCTAACCCGAGCTTAATTTGCTCCTAGGTAtaataa                                                    <  1:629780/249‑1 (MQ=255)                                                                                                                                                                            cACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTGAGCTCTATACTGCACAGGGTTTAAATCGTCTAACCCGAGCTTAATTTGCTCCTAGGTAtaataa                                                    <  1:311235/249‑1 (MQ=255)                                                                                                                                                                                              ggTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTGAGCTCTATACTGCACAGGGTTTAAATCGTCTAACCCGAGCTTAAttt                                                                     <  1:551990/214‑1 (MQ=255)                                                                                                                                                                                              ggTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTGAGCTCTATACTGCACAGGGTTTAAATCGTCTAACCCGAGCTTAATTTGCTCCTAGGTATAATAACg                                                  <  1:585281/233‑1 (MQ=255)                                                                                                                                                                                                tgtTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTGAGCTCTATACTGCACAGGGTTTAAATCGTCTAACCCGAGCTTAAttt                                                                     >  1:852937/1‑212 (MQ=255)                                                                                                                                                                                                 gtTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTGAGCTCTATACTGcac                                                                                                     <  1:335815/179‑1 (MQ=255)                                                                                                                                                                                                 gtTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTGAGCTCTATACTGc                                                                                                       <  1:730421/177‑1 (MQ=255)                                                                                                                                                                                                        gTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTGAGCTCTATACTGc                                                                                                       <  1:711783/170‑1 (MQ=255)                                                                                                                                                                                                         ttCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTGAGCTCTATACTGCACAGGGTTTAAATCGTCTAACCCGAGCTTAATTTGCTCCTAGGTATAATAACGAATATATCATGTATCATCCATTCTGAtt                      >  1:120143/1‑250 (MQ=255)                                                                                                                                                                                                                            gCCATTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTGAGCTCTATACTGCACAGGGTTTAAATCGTCTAACCCGAGCTTAATTTGCTCCTAGGTATAATAACGAATATATCATGTATCATCCATTCTGATTCATCAATGTCCTTAAACTGa  >  1:82699/1‑251 (MQ=255)                                                                                                                                                                                                                               aTTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTGAGCTCTATACTGCACAGGGTTTAAATCGTCTAACCCGAGCTTAATTTGCTCCTAGGTATAATAACGAATATATCATg                                       <  1:726918/211‑1 (MQ=255)                                                                                                                                                                                                                               aTTTACTTCATCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTGAGCTCTATACTGCACAGGGTTTAAATCGTCTAACCCGAGCTTAATTTGCTCCTAGGTATAATAACGAATATATCATGTATCATCCATTCTGATTCATCAATGt             <  1:582428/237‑1 (MQ=255)                                                                                                                                                                                                                                        aTCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTGAGCTCtatac                                                                                                          <  1:259967/135‑2 (MQ=255)                                                                                                                                                                                                                                        aTCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTGAGCTCTATACTGCACAGGGTTTAAATCGTCTAACCCGAGCTTAATTTGCTCCTAGGTATAATAACGAATATATCATGTat                                    >  1:135728/1‑205 (MQ=255)                                                                                                                                                                                                                                        aTCCTGAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTGAGCTCTATACTGCACAGGGTTTAAATCGTCTAACCCGAGCTTAATCTGCTCCTAGGTATAATAACGAatatat                                           <  1:823751/198‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                          CCTATGTGAGAGTAGGTCACTGCTAATTCTCATTCTGGAGTGGTAGTTCAGCTGGTTAGAATACCTGCCTGTCACGCAGGGGGTCGCGGGTTCGAGTCCCGTCCATTCCGCCAACTTTTGTAACCTTTTCAGGTTATACATACCAATAGGGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGACTCTCCGCCCCTGCCATTTACTTCATCCTAAACTGAAAATTGTCTCTATCCGTTTTAAATCTAATTTCTTTAAAAAAGAAAACTGTACTTTAAAATCAGATTAAAAAAGTGATTTGAGCCCCAAAAGTTGGACAGATAACTAAATATTGAGCTCTATATTGCACAGGGTTTAAATCGTCTAACCCGAGCTTAATTTGCTCCTAGGTATAATAACGAATATATCATGTATCATCCATTCTGATTCATCAATGTCCTTAAACTGA  >  1/1040701‑1041170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑