Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,030,847	G→A	intergenic (‑24391/‑4372)	unknown ← / → unknown	tRNA‑Gly‑GCC/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,030,847	0	G	A	100.0%	94.1 / NA	36	intergenic (‑24391/‑4372)	unknown/unknown	tRNA‑Gly‑GCC/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/20);  total (16/20)

ACTTGAGTCTTCAAGAGACTTTAAAAAGAATGTTGAAGCTGTAAAGCAAAATTTAAGTGAGAAAAGTCAATCAATCTCTACTGAAAATCACTCAATAAAATTCAAGCTATAAGAGATAAAATAGGGTAGGATATTTTTAAATTGAGATATTAGATGGAAATATCAAATTCCCATTTACATAAACTTCAAAGTTTTTAAGAGCTAGTGGATTTTAAGAGGTATAGATCGGGCTTGTTGGATCCACCCGTGCCAGTACCGTTTGGCAAAATTATGAAGAGC‑AGATTAAACAACTTAAAAATGGAGGTTGTTTTAAAATTTTTACAGGCAAGAATTCTGGTTCAAAAGCTAAAAACCGTGCAAGACTTGATGAATTATTAAATTTCGTGCGATAAGGGAGTACCGTTGTAGTAAAAAATTAGATCGCCTTGGTCGTAGTACTAGTCA  >  1/1030647‑1031090                                                                                                                                                                                                         |                                                                                                                                                                                                                                                     aCTTGAGTCTTCAAGAGACTTTAAAAAGAATGTTGAAGCTGTAAAGCAAAATTTAAGTGAGAAAAGTCAATCAATCTCTACTGAAAATCACTCAATAAAATTCAAGCTATAAGAGATAAAATAGGGTAGGATATTTTTAAATTGAGATATTAGATGGAAATATCAAATTCCCATTTACATAAACTTCAAAGTTTTTAAGAACTAGTGGATTTTAAGAGGTATAGATCGGGCTTGTTGGATCCACCCGTGcc                                                                                                                                                                                                    <  1:167806/251‑1 (MQ=255)         cTTCAAGAGACTTTAAAAAGAATGTTGAAGCTGTAAAGCAAAATTTAAGTGAGAAAAGTCAATCAATCTCTACTGAAAATCACTCAATAAAATTCAAGCTATAAGAGATAAAATAGGGTAGGATATTTTTAAATTGAGATATTAGATGGAAATATCAAATTCCCATTTACATAAACTTCAAAGTTTTTAAGAACTAGTGGATTTTAAGAGGTATAGATCGGGCTTGTTGGATc                                                                                                                                                                                                              <  1:617257/233‑1 (MQ=255)                                       cTGTAAAGCAAAATTTAAGTGAGAAAAGTCAATCAATCTCTACTGAAAATCACTCAATAAAATTCAAGCTATAAGAGATAAAATAGGGTAGGATATTTTTAAATTGAGATATTAGATGGAAATATCAAATTCCCATTTACATAAACTTCAAAGTTTTTAAGAACTAGTGGATTTTAAGAGGTATAGATCGGGCTTGTTGGATCCACCCGTGCCAGTACCGTTTGGCAAAATTATGAAGAGC‑AGATTAaaca                                                                                                                                                             <  1:127221/251‑1 (MQ=255)                                                                                             cAATAAAATTCAAGCTATAAGAGATAAAATAGGGTAGGATATTTTTAAATTGAGATATTAGATGGAAATATCAAATTCCCATTTACATAAACTTCAAAGTTTTTAAGAACTAGTGGATTTTAAGAGGTATAg                                                                                                                                                                                                                               >  1:281045/1‑132 (MQ=255)                                                                                                         aGCTATAAGAGATAAAATAGGGTAGGATATTTTTAAATTGAGATATTAGATGGAAATATCAAATTCCCATTTACATAAGCTTCAAAGTTTTTAAGAACTAGTGGATTTTAAGAGGTATAGATCGGGCTTGTTGGATCCACCCGTGCCAGTACCGTTTGGCAAAATTATGAAGAGC‑AGATTAAACAACTTAAAAATGGAGGTTGTTTTAAAATTTTTACAGGCAAGAAt                                                                                                                  <  1:339906/228‑1 (MQ=255)                                                                                                         aGCTATAAGAGATAAAATAGGGTAGGATATTTTTAAATTGAGATATTAGATGGAAATATCAAATTCCCATTTACATAAACTTCAAAGTTTTTAAGAACTAGTGGATTTTAAGAGGTATAGATCGGGCTTGTTGGATc                                                                                                                                                                                                              <  1:690981/137‑1 (MQ=255)                                                                                                         aGCTATAAGAGATAAAATAGGGTAGGATATTTTTAAATTGAGATATTAGATGGAAATATCAAATTCCCATTTACATAAACTTCAAAGTTTTTAAGAACTAGTGGATTTTAAGAGGTATAGATCGGGCTTGTTGGATCCACCCGTGCCAGTACCGTTTGGCAAAATTATGAAGAGC‑AGATTAAACAACTTAAAAATGGAGGTTGTTTTAAAATTTTTACAGGCAAGAATTCTGGTTCAAAAGCTAAAAACCg                                                                                           >  1:826216/1‑251 (MQ=255)                                                                                                          gCTATAAGAGATAAAATAGGGTAGGATATTTTTAAATTGAGATATTAGATGGAAATATCAAATTCCCATTTACATAAACTTCAAAGTTTTTAAGAACTAGTGGATTTTAAGAGGTATAGATCGGGCTTGTTGGATCCACCCGTGCCAGTACCGTTTGGCAAAATTATGa                                                                                                                                                                             <  1:427422/169‑1 (MQ=255)                                                                                                          gCTATAAGAGATAAAATAGGGTAGGATATTTTTAAATTGAGATATTAGATGGAAATATCAAATTCCCATTTACATAAACTTCAAAGTTTTTAAGAACTAGTGGATTTTAAGAGGTATAGATCGGGCTTGTTGGATCCACCCGTGCCAGTACCGTTTGGCAAAATTATGAAGAGC‑AGATTAAACAACTTAAAAATGGAGGTTGTTTTAAAATTTTTACAGGCAAGAAt                                                                                                                  >  1:871422/1‑227 (MQ=255)                                                                                                           cTATAAGAGATAAAATAGGGTAGGATATTTTTAAATTGAGATATTAGATGGAAATATCAAATTCCCATTTACATAAACTTCAAAGTTTTTAAGAACTAGTGGATTTTAAGAGGTATAGATCGGGCTTGTTGGATCCACCCGTGCCAGTACCGTTTGGCAAAATTATGAAGAGC‑AGATTAAACAACTTAAAAATGGAGGTTGTTTTAAAATTTTTACAGGCAAGAATTCTGGTTCAAAAGCTAAAAACCGTg                                                                                         >  1:381859/1‑251 (MQ=255)                                                                                                                            gggTAGGATATTTTTAAATTGAGATATTAGATGGAAATATCAAATTCCCATTTACATAAACTTCAAAGTTTTTAAGAACTAGTGGATTTTAAGAGGTATAGATCGGGCTTGTTGGATCCACCCGTGCCAGTACCGTTTGGCAAAATTATGAAGAGC‑AGATTAAACAACTTAAAAATGGAGGTTGTTTTAAAATTTTTACAGGCAAGAATTCTGGTTCAAAAGCTAAAAACCGTGCAAGACttgatgaattg                                                                        >  1:261436‑M1/1‑240 (MQ=255)                                                                                                                            gggTAGGATATTTTTAAATTGAGATATTAGATGGAAATATCAAATTCCCATTTACATAAACTTCAAAGTTTTTAAGAACTAGTGGATTTTAAGAGGTATAGATCGGGCTTGTTGGATCCACCCGTGCCAGTACCGTTTGGCAAAATTATGAAGAGC‑AGATTAAACAACTTAAAAATGGAGGTTGTTTTAAAATTTTTACAGGCAAGAATTCTGGTTCAAAAGCTAAAAACCGTGCAAGACttgatgaattg                                                                        >  1:729828‑M1/1‑240 (MQ=255)                                                                                                                               tAGGATATTTTTAAATTGAGATATTAGATGGAAATATCAAATTCCCATTTACATAAACTTCAAAGTTTTTAAGAACTAGTGGATTTTAAGAGGTATAGATCGGGCTTGTTGGATCCACCCGTGCCAGTACCGTTTGGCAAAATTATGAAGAGC‑AGATTAAACAACTTAAAAATGGAGGTTGTTTTAAAATTTTTACAGGCAAGAATTCTGGTTCAAAAGCTAAAAACCGTGCAAGACttgatgaattgatg                                                                     <  1:79870‑M1/251‑15 (MQ=255)                                                                                                                                 ggATATTTTTAAATTGAGATATTAGATGGAAATATCAAATTCCCATTTACATAAACTTCAAAGTTTTTAAGAACTAGTGGATTTTAAGAGGTATAGATCGGGCTTGTTGGATCCACCCGTGCCAGTACCGTTTGGCAAAATTATGAAGAGC‑AGATTAAACAACTTAAAAATGGAGGTTGTTTTAAAATTTTTACAGGCAAGAATTCTGGTTCAAAAGCTAAAAACCGTGCAAGACttgatgaattgatgaa                                                                   >  1:496949‑M1/1‑235 (MQ=255)                                                                                                                                      tttttAAATTGAGATATTAGATGGAAATATCAAATTCCCATTTACATAAACTTCAAAGTTTTTAAGAACTAGTGGATTTTAAGAGGTATAGATCGGGCTTGTTGGATCCACCCGTGCCAGTACCGTTTGGCAAAATTATGAAGAGc                                                                                                                                                                        >  1:553044/1‑146 (MQ=255)                                                                                                                                      tttttAAATTGAGATATTAGATGGAAATATCAAATTCCCATTTACATAAACTTCAAAGTTTTTAAGAACTAGTGGATTTTAAGAGGTATAGATCGGGCTTGTTGGATCCACCCGTGCCAGTACCGTTTGGCAAAATTATGAAGAGC‑AGATTAAACAACTTAAAAATGGAGGTTGTTTTAAAATTTTTACAGGCAAGAATTCTGGTTCAAAAGCTAAAAACCGTGCAAGACttgatgaattgatgaattatt                                                              >  1:749897‑M1/1‑230 (MQ=255)                                                                                                                                          tAAATTGAGATATTAGATGGAAATATCAAATTCCCATTTACATAAACTTCAAAGTTTTTAAGAACTAGTGGATTTTAAGAGGTATAGATCGGGCTTGTTGGATCCACCCGTGCCAGTACCGTTTGGCAAAATTATGAAGAGC‑AGATTAAACAACTTAAAAATGGAGGTTGTTTTAAAATTTTTACAGGCAAGAATTCTGGTTCAAAAGCTAAAAACCGTGCAAGACttgatgaattgatgaattattaaat                                                          <  1:361678‑M1/251‑26 (MQ=255)                                                                                                                                                             gAAATATCAAATTCCCATTTACATAAACTTCAAAGTTTTTAAGAACTAGTGGATTTTAAGAGGTATAGATCGGGCTTGTTGGATCCACCCGTGCCAGTACCGTTTGGCAAAATTATGAAGAGC‑AGATTAAACAACTTaaaaa                                                                                                                                                    >  1:692471/1‑142 (MQ=255)                                                                                                                                                             gAAATATCAAATTCCCATTTACATAAACTTCAAAGTTTTTAAGAACTAGTGGATTTTAAGAGGTATAGATCGGGCTTGTTGGATCCACCCGTGCCAGTACCGTTTGGCAAAATTATGAAGAGC‑AGATTAAACAACTTAAAAATGGAGGTTGTTTTAAAATTTTTACAGGCAAGAATTCTGGTTCAAAAGCTAAAAACCGTGCAAGAc                                                                                   <  1:303647/207‑1 (MQ=255)                                                                                                                                                               aaTATCAAATTCCCATTTACATAAACTTCAAAGTTTTTAAGAACTAGTGGATTTTAAGAGGTATAGATCGGGCTTGTTGGATCCACCCGTGCCAGTACCGTTTGGCAAAATTATGAAGAGC‑AGATTAAACAACTTAAAAATGGAGGTTGTTTTAAAATTTTTACAGGCAAGAATTCTGGTTCAAAAGCTAAAAACCGTGCAAGACttgatgaattgatgaattattaaatttcgtgcgataagggagtacc                                     <  1:398455‑M1/251‑47 (MQ=255)                                                                                                                                                                  atCAAATTCCCATTTACATAAACTTCAAAGTTTTTAAGAACTAGTGGATTTTAAGAGGTATAGATCGGGCTTGTTGGATCCACCCGTGCCAGTACCGTTTGGCAAAATTATGAAGAGC‑AGATTAAACAACTTAAAAATGGAGGTTGTTTTAAAATTTTTACAGGCAAGAATTCTGGTTCAAAAGCTAAAAACCGTGCAAGACttgatgaattgatgaattattaaatttcgtgcgataaggg                                           <  1:607913‑M1/242‑41 (MQ=255)                                                                                                                                                                  atCAAATTCCCATTTACATAAACTTCAAAGTTTTTAAGAACTAGTGGATTTTAAGAGGTATAGATCGGGCTTGTTGGATCCACCCGTGCCAGTACCGTTTGGCAAAATTATGAAGAGC‑AGATTAAACAACTTAAAAATGGAGGTTGTTTTAAAATTTTTACAGGCAAGAATTCTGGTTCAAAAGCTAAAAACCGTGCAAGACttgatgaattgatgaattattaaatttcgtgcgataaggg                                           <  1:139307‑M1/242‑41 (MQ=255)                                                                                                                                                                   tCAAATTCCCATTTACATAAACTTCAAAGTTTTTAAGAACTAGTGGATTTTAAGAGGTATAGATCGGGCTTGTTGGATCCACCCGTGCCAGTACCGTTTGGCAAAATTATGAAGAGC‑AGATTAAACAACTTAAAAATGGAGGTTGTTTTAAAATTTTTACAGGCAAGAATTCTGGTTCAAAAGCTAAAAACCGTGCAAGACttgatgaattgatgaattattaaatttcgtgcgataagggagtaccgttg                                 >  1:848973‑M1/1‑201 (MQ=255)                                                                                                                                                                      aaTTCCCATTTACATAAACTTCAAAGTTTTTAAGAACTAGTGGATTTTAAGAGGTATAGATCGGGCTTGTTGGATCCACCCGTGCCAGTACCGTTTGGCAAAATTATGAAGAGC‑AGATTAAACAACTTAAAAATGGAGGTTGTTTTAAAATTTTTACAGGCAAGAATTCTGGTTCAAAAGCTAAAAACCGTGCAAGACttgatga                                                                            >  1:821273‑M1/1‑198 (MQ=255)                                                                                                                                                                       aTTCCCATTTACATAAACTTCAAAGTTTTTAAGAACTAGTGGATTTTAAGAGGTATAGATCGGGCTTGTTGGATCCACCCGTGCCAGTACCGTTTGGCAAAATTATGAAGAGC‑AGATTAAACAACTTAAAAATGGAGGTTGTTTTAAAATTTTTACAGGCAAGAATTCTGGTTCAAAAGCTAAAAACCGTGCAAGACttgatgaattgatgaattattaaat                                                          >  1:200847‑M1/1‑197 (MQ=255)                                                                                                                                                                          cccATTTACATAAACTTCAAAGTTTTTAAGAACTAGTGGATTTTAAGAGGTATAGATCGGGCTTGTTGGATCCACCCGTGCCAGTACCGTTTGGCAAAATTATGAAGAGCAAGATTAAACAACTTAAAAATGGAGGTTGTTTTAAAATTTTTACAGGCAAGAATTCTGGTTCAAAAGCTAAAAACCGTGCAAGACttgatgaattgatgaattatta                                                             <  1:1143‑M1/217‑23 (MQ=255)                                                                                                                                                                                 aCATAAACTTCAAAGTTTTTAAGAACTAGTGGATTTTAAGAGGTATAGATCGGGCTTGTTGGATCCACCCGTGCCAGTACCGTTTGGCAAAATTATGAAGAGC‑AGATTAAACAACTTAAAAATGGAGGTTGTTTTAAAATTTTTACAGGCAAGAATTCTGGTTCAAAAGCTAAAAACCGTGCaa                                                                                      <  1:411145/184‑1 (MQ=255)                                                                                                                                                                                 aCATAAACTTCAAAGTTTTTAAGAACTAGTGGATTTTAAGAGGTATAGATCGGGCTTGTTGGATCCACCCGTGCCAGTACCGTTTGGCAAAATTATGAAGAGC‑AGATTAAACAACTTAAAAATGGAGGTTGTTTTAAAATTTTTACAGGCAAGAATTCTGGTTCAAAAGCTAAAAACCGTGCAAGACttgatgaattgatgaattattaaatttcgtgcgataagggag                                         <  1:68508‑M1/229‑43 (MQ=255)                                                                                                                                                                                 aCATAAACTTCAAAGTTTTTAAGAACTAGTGGATGTTAAGAGGTATAGATCGGGCTTGTTGGATCCACCCGTGCCAGTACCGTTTGGCAAAATTATGAAGAGC‑AGATTAAACAACTTAAAAATGGAGGTTGTTTTAAAATTTTTACAGGCAAGAATTCTGGTTCAAAAGCGAAAAAc                                                                                             <  1:407680/177‑1 (MQ=255)                                                                                                                                                                                   aTAAACTTCAAAGTTTTTAAGAACTAGTGGATTTTAAGAGGTATAGATCGGGCTTGTTGGATCCACCCGTGCCAGTACCGTTTGGCAAAATTATGAAGAGC‑AGATTAAACAACTTAAAAATGGAGGTTGTTTTAAAATTTTTACAGGCAAGAATTCTGGTTCAAAAGCTAAAAACCGTGCAAGACttgatgaattgatgaattattaaatttcgtgcgataagggagtaccgttgtagtaaaaaattagat                 <  1:95618‑M1/251‑67 (MQ=255)                                                                                                                                                                                        cTTCAAAGTTTTTAAGAACTAGTGGATTTTAAGAGGTATAGATCGGGCTTGTTGGATCCACCCGTGCCAGTACCGTTTGGCAAAATTATGAAGAGC‑AGATTAAACAACTTAAAAATGGAGGTTGTTTTAAAATTTTTACAGGCAAGAATTCTGGTTCAAAAGCTAAAAACCGTGCAAGACttgatgaattgatgaattattaaatttcgtgcgataagggagtaccgttgtagtaaaaaattagatcgc              >  1:141852‑M1/1‑180 (MQ=255)                                                                                                                                                                                        cTTCAAAGTTTTTAAGAACTAGTGGATTTTAAGAGGTATAGATCGGGCTTGTTGGATCCACCCGTGCCAGTACCGTTTGGCAAAATTATGAAGAGC‑AGATTAAACAACTTAAAAATGGAGGTTGTTTTAAAATTTTTACAGGCAAGAATTCTGGTTCAAAAGCTAAAAACCGTGCAAGACttgatgaattgatgaattattaaatttcgtgcgataaggg                                           <  1:623182‑M1/220‑41 (MQ=255)                                                                                                                                                                                             aaGTTTTTAAGAACTAGTGGATTTTAAGAGGTATAGATCGGGCTTGTTGGATCCACCCGTGCCAGTACCGTTTGGCAAAATTATGAAGAGC‑AGATTAAACAACTTAAAAATGGAGGTTGTTTTAAAATTTTTACAGGCAAGAATTCTGGTTCAAAAGCTAAAAACCGTGCAAGACttgatgaattgatgaattattaaatttcgtgcgataagggagtaccgttgtagtaaaaaattagatcgccttggtc       <  1:710039‑M1/251‑77 (MQ=255)                                                                                                                                                                                               gTTTTTAAGAACTAGTGGATTTTAAGAGGTATAGATCGGGCTTGTTGGATCCACCCGTGCCAGTACCGTTTGGCAAAATTATGAAGAGC‑AGATTAAACAACTTAAAAATGGAGGTTGTTTTAAAATTTTTACAGGCAAGAATTCTGGTTCAAAAGCTAAAAACCGTGCAAGACttgatgaattgatgaattattaaatttcgtgcgataagggagtaccgttgtagtaaaaaattagatcgccttggtcgt     >  1:831041‑M1/1‑173 (MQ=255)                                                                                                                                                                                                  tttAAGAACTAGTGGATTTTAAGAGGTATAGATCGGGCTTGTTGGATCCACCCGTGCCAGTACCGTTTGGCAAAATTATGAAGAGC‑AGATTAAACAACTTAAAAATGGAGGTTGTTTTAAAATTTTTACAGGCAAGAATTCTGGTTCAAAAGCTAAAAACCGTGCAAGACttgatgaattgatgaattattaaatttcgtgcgataagggagtaccgttgtagtaaaaaattagatcgccttggtcgtagt  >  1:724740‑M1/1‑170 (MQ=255)                                                                                                                                                                                                  tttAAGAACTAGTGGATTTTAAGAGGTATAGATCGGGCTTGTTGGATCCACCCGTGCCAGTACCGTTTGGCAAAATTATGAAGAGC‑AGATTAAACAACTTAAAAATGGAGGTTGTTTTAAAATTTTTACAGGCAAGAATTCTGGTTCAAAAGCTAAAAACCGTGCAAGACttgatgaattgatgaattattaaatttcgtgcgataagggagtaccgttgtagtaaaaaattagatcgccttggtcgtagt  <  1:425202‑M1/251‑82 (MQ=255)                                                                                                                                                                                                         |                                                                                                                                                                                                                                                     ACTTGAGTCTTCAAGAGACTTTAAAAAGAATGTTGAAGCTGTAAAGCAAAATTTAAGTGAGAAAAGTCAATCAATCTCTACTGAAAATCACTCAATAAAATTCAAGCTATAAGAGATAAAATAGGGTAGGATATTTTTAAATTGAGATATTAGATGGAAATATCAAATTCCCATTTACATAAACTTCAAAGTTTTTAAGAGCTAGTGGATTTTAAGAGGTATAGATCGGGCTTGTTGGATCCACCCGTGCCAGTACCGTTTGGCAAAATTATGAAGAGC‑AGATTAAACAACTTAAAAATGGAGGTTGTTTTAAAATTTTTACAGGCAAGAATTCTGGTTCAAAAGCTAAAAACCGTGCAAGACTTGATGAATTATTAAATTTCGTGCGATAAGGGAGTACCGTTGTAGTAAAAAATTAGATCGCCTTGGTCGTAGTACTAGTCA  >  1/1030647‑1031090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑