Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,022,826	T→C	intergenic (‑16370/‑12393)	unknown ← / → unknown	tRNA‑Gly‑GCC/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,022,826	0	T	C	100.0%	179.9 / NA	63	intergenic (‑16370/‑12393)	unknown/unknown	tRNA‑Gly‑GCC/SSU rRNA ## 16S rRNA, small subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/25);  total (38/25)

TTTGGCGGCACCACAAAAAATGGCATTGATTGTTCCGGCTTTATGCAAACCACCTTTTTAGATGCGTTTGGTGTGACATTGCCGCGTTCGACTTCCGAACAGCGTTATGTCGGTAAAGCGATTCAAAAGAAAGAACTGAAAGTGGGCGATTTGGTTTTTTTCCGTAGAAATAATCATGTAGGCGTATACGTCGGCAACGATCAGTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCTTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGTTCTTTAAGTTTGATTAGATTGAATAAAAAGTATTCTGCATCCCAATCAGATACCACAACCAACTAAACTATGGATTTTTATGGTCAAACATTATTCAATCGAATTCAAACATCATATCATCCGCCGACGTAAGCAAGCGGAAAAAAACGGTCATATTGCGTCAAACTTACTCACGTGCAATCTGATTACCAGA  >  1/1022581‑1023066                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 tttGGCGGCACCACAAAAAATGGCATTGATTGTTCCGGCTTTATGCAAACCACCTTTTTAGATGCGTTTGGTGTGACATTGCCGCGTTCGACTTCCGAACAGCGTTATGTCGGTAAAGCGATTCAAAAGAAAGAACTGAAAGTGGGCGATTTGGTTTTTTTCCGTAGAAATAATCATGTAGGCGTATACGTCGGCAACGATCAGTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAg                                                                                                                                                                                                                                             <  1:729667/251‑1 (MQ=255)           ccgcaAAAAATGGCATTTATTGTTCCGGCTTTATGCAAACCACCTTTTTAGATGCGTTTGGTGTGACATTGCCGCGTTCGGCTTCCGAACAGCGTTATGTCGGTAAAGCGATTCAAAAGAAAGAACTGAAAGTGGGCGATTTGGTTTTTTTCCGTAGAAATAATCATGTAGGCGTATACGTCGGCAACGATCAGTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTAc                                                                                                                                                                                                                                   <  1:777883/248‑1 (MQ=255)               aaaaaaTGGCATTGATTGTTCCGGCTTTATGCAAACCACCTTTTTAGATGCGTTTGGTGTGACATTGCCGCGTTCGACTTCCGAACAGCGTTATGTCGGTAAAGCGATTCAAAAGAAAGAACTGAAAGTGGGCGATTTGGTTTTTTTCCGTAGAAATAATCATGTAGGCGTATACGTCGGCAACGATCAGTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTggg                                                                                                                                                                                                                               <  1:311982/251‑1 (MQ=255)                              ttgTTCCGGCTTTATGCAAACCACCTTTTTAGATGCGTTTGGTGTGACATTGCCGCGTTCGACTTCCGAACAGCGTTATGTCGGTAAAGCGATTCAAAAGAAAGAACTGAAAGTGGGCGATTTGGTTTTTTTCCGTAGAAATAATCATGTAGGCGTATACGTCGGCAACGATCAGTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATAccc                                                                                                                                                                                                                <  1:634016/251‑1 (MQ=255)                              ttgTTCCGGCTTTATGCAAACCACCTTTTTAGATGCGTTTGGTGTGACATTGCCGCGTTCGACTTCCGAACAGCGTTATGTCGGTAAAGCGATTCAAAAGAAAGAACTGAAAGTGGGCGATTTGGTTTTTTTCCGTAGAAATAATCATGTAGGCGTATACGTCGGCAACGATCAGTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATAccc                                                                                                                                                                                                                <  1:373532/251‑1 (MQ=255)                              ttgTTCCGGCTTTATGCAAACCACCTTTTTAGATGCGTTTGGTGTGACATTGCCGCGTTCGACTTCCGAACAGCGTTATGTCGGTAAAGCGATTCAAAAGAAAGAACTGAAAGTGGGCGATTTGGTTTTTTTCCGTAGAAATAATCATGTAGGCGTATACGTCGGCAACGATCAGTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATAccc                                                                                                                                                                                                                <  1:771726/251‑1 (MQ=255)                                      gCTTTATGCAAACCACCTTTTTAGATGCGTTTGGTGTGACATTGCCGCGTTCGACTTCCGAACAGCGTTATGTCGGTAAAGCGATTCAAAAGAAAGAACTGAAAGTGGGCGATTTGGTTTTTTTCCGTAGAAATAATCATGTAGGCGTATACGTCGGCAACGATCAGTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATAccc                                                                                                                                                                                                                <  1:772085/243‑1 (MQ=255)                                                   caccTTTTTAGATGCGTTTGGTGTGACATTGCCGCGTTCGACTTCCGAACAGCGTTATGTCGGTAAAGCGATTCAAAAGAAAGAACTGAAAGTGGGCGATTTGGTTTTTTTCCGTAGAAATAATCATGTAGGCGTATACGTCGGCAACGATCAGTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATAccc                                                                                                                                                                                                                >  1:256286/1‑230 (MQ=255)                                                     ccTTTTTAGATGCGTTTGGTGTGACATTGCCGCGTTCGACTTCCGAACAGCGTTATGTCGGTAAAGCGATTCAAAAGAAAGAACTGAAAGTGGGCGATTTGGTTTTTTTCCGTAGAAATAATCATGTAGGCGTATACGTCGGCAACGATCAGTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGTTCTTTAAGtt                                                                                                                                                                                         <  1:873537/251‑1 (MQ=255)                                                        ttttAGATGCGTTTGGTGTGACATTGCCGCGTTCGACTTCCGAACAGCGTTATGTCGGTAAAGCGATTCAAAAGAAAGAACTGAAAGTGGGCGATTTGGTTTTTTTCCGTAGAAATAATCATGTAGGCGTATACGTCGGCAACGATCAGTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGTTCTTTAAGtt                                                                                                                                                                                         <  1:523957/248‑1 (MQ=255)                                                        ttttAGATGCGTTTGGTGTGACATTGCCGCGTTCGACTTCCGAACAGCGTTATGTCGGTAAAGCGATTCAAAAGAAAGAACTGAAAGTGGGCGATTTGGTTTTTTTCCGTAGAAATAATCATGTAGGCGTATACGTCGGCAACGATCAGTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGTTCTTTAAGTTTGa                                                                                                                                                                                      >  1:509321/1‑251 (MQ=255)                                                                     tGGTGTGACATTGCCGCGTTCGACTTCCGAACAGCGTTATGTCGGTAAAGCGATTCAAAAGAAAGAACTGAAAGTGGGCGATTTGGTTTTTTTCCGTAGAAATAATCATGTAGGCGTATACGTCGGCAACGATCAGTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCg                                                                                                                                                                                                                                           <  1:451889/185‑1 (MQ=255)                                                                       gtgtGACATTGCCGCGTTCGACTTCCGAACAGCGTTATGTCGGTAAAGCGATTCAAAAGAAAGAACTGAAAGTGGGCGATTTGGTTTTTTTCCGTAGAAATAATCATGTAGGCGTATACGTCGGCAACGATCAGTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGTTCTTTAAGTTTGATTAGATTGAATaaaa                                                                                                                                                                       >  1:355086/1‑251 (MQ=255)                                                                           gACATTGCCGCGTTCGACTTCCGAACAGCGTTATGTCGGTAAAGCGATTCAAAAGAAAGAACTGAAAGTGGGCGATTTGGTTTTTTTCCGTAGAAATAATCATGTAGGCGTATACGTCGGCAACGATCAGTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGTTCTTTAAGTTTGATTAGATTGAATaatgtttg                                                                                                                                                                   >  1:380264/1‑245 (MQ=255)                                                                            aCATTGCCGCGTTCGACTTCCGAACAGCGTTATGTCGGTAAAGCGATTCAAAAGAAAGAACTGAAAGTGGGCGATTTGGTTTTTTTCCGTAGAAATAATCATGTAGGCGTATACGTCGGCAACGATCAGTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATAc                                                                                                                                                                                                                  >  1:9528/1‑203 (MQ=255)                                                                                   cgcgTTCGACTTCCGAACAGCGTTATGTCGGTAAAGCGATTCAAAAGAAAGAACTGAAAGTGGGCGATTTGGTTTTTTTCCGTAGAAATAATCATGTAGGCGTATACGTCGGCAACGATCAGTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGTTCTTTAAGTTTGATTAGATTGAATAAAAAGTATTCTGCAt                                                                                                                                                           <  1:347694/251‑1 (MQ=255)                                                                                                      gCGTTATGTCGGTAAAGCGATTCAAAAGAAAGAACTGAAAGTGGGCGATTTGGTTTTTTTCCGTAGAAATAATCATGTAGGCGTATACGTCGGCAACGATCAGTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGTTCTTTAAg                                                                                                                                                                                           >  1:849099/1‑200 (MQ=255)                                                                                                      gCGTTATGTCGGTAAAGCGATTCAAAAGAAAGAACTGAAAGTGGGCGATTTGGTTTTTTTCCGTAGAAATAATCATGTAGGCGTATACGTCGGCAACGATCAGTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGTTCTTTAAGTTTGAtt                                                                                                                                                                                    <  1:344684/207‑1 (MQ=255)                                                                                                      gCGTTATGTCGGTAAAGCGATTCAAAAGAAAGAACTGAAAGTGGGCGATTTGGTTTTTTTCCGTAGAAATAATCATGTAGGCGTATACGTCGGCAACGATCAGTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGTTCTTTAAGTTTGATTAGAt                                                                                                                                                                                >  1:677673/1‑211 (MQ=255)                                                                                                      gCGTTATGTCGGTAAAGCGATTCAAAAGAAAGAACTGAAAGTGGGCGATTTGGTTTTTTTCCGTAGAAATAATCATGTAGGCGTATACGTCGGCAACGATCAGTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGTTCTTTAAGTTTGATTAGATTGAATAAAAAGTATTCTGCATCCCAATCAGATACCAcaa                                                                                                                                         >  1:139891/1‑250 (MQ=255)                                                                                                               cGGTAAAGCGATTCAAAAGAAAGAACTGAAAGTGGGCGATTTGGTTTTTTTCCGTAGAAATAATCATGTAGGCGTATACGTCGGCAACGATCAGTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGTTCTTTAAGTTTGATTAGATTGAATAAAAAGTATTCTGCATCCCAATCAGATAc                                                                                                                                              >  1:76873/1‑236 (MQ=255)                                                                                                               cGGTAAAGCGATTCAAAAGAAAGAACTGAAAGTGGGCGATTTGGTTTTTTTCCGTAGAAATAATCATGTAGGCGTATACGTCGGCAACGATCAGTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGTTCTTTAAGTTTGATTAGATTGAATAAAAAGTATTCTGCATCCCAATCAGATACCACAACCaactaaac                                                                                                                               <  1:374286/251‑1 (MQ=255)                                                                                                               cGGTAAAGCGATTCAAAAGAAAGAACTGAAAGTGGGCGATTTGGTTTTTTTCCGTAGAAATAATCATGTAGGCGTATACGTCGGCAACGATCAGTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGTTCTTTAAGTTTGATTAGATTGAATAAAAAGTATTCTGCATCCCAATCAGATACCACAACCaactaaac                                                                                                                               <  1:678161/251‑1 (MQ=255)                                                                                                               cGGTAAAGCGATTCAAAAGAAAGAACTGAAAGTGGGCGATTTGGTTTTTTTCCGTAGAAATAATCATGTAGGCGTATACGTCGGCAACGATCAGTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGTTCTTTAAGTTTGATTAGATTGAATAAAAAGTATTCTGCATCCCAATCAGATACCACAACCaactaaac                                                                                                                               <  1:279782/251‑1 (MQ=255)                                                                                                               cGGTAAAGCGATTCAAAAGAAAGAACTGAAAGTGGGCGATTTGGTTTTTTTCCGTAGAAATAATCATGTAGGCGTATACGTCGGCAACGATCAGTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGTTCTTTAAGTTTGATTAGATTGAATAAAAAGTATTCTGCATCCCAATCAGATACCACAACCaactaaac                                                                                                                               <  1:651857/251‑1 (MQ=255)                                                                                                               cGGTAAAGCGATTCAAAAGAAAGAACTGAAAGTGGGCGATTTGGTTTTTTTCCGTAGAAATAATCATGTAGGCGTATACGTCGGCAACGATCAGTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGTTCTTTAAGTTTGATTAGATTGAATAAAAAGTATTCTGCATCCCAATCAGATACCACAACCaactaaac                                                                                                                               <  1:220648/251‑1 (MQ=255)                                                                                                                 gTAAAGCGATTCAAAAGAAAGAACTGAAAGTGGGCGATTTGGTTTTTTTCCGTAGAAATAATCATGTAGGCGTATACGTCGGCAACGATCAGTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATAccc                                                                                                                                                                                                                >  1:347656/1‑168 (MQ=255)                                                                                                                         aTTCAAAAGAAAGAACTGAAAGTGGGCGATTTGGTTTTTTTCCGTAGAAATAATCATGTAGGCGTATACGTCGGCAACGATCAGTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGTTCTTTAAGTTTGATTAGATTGAATAAAAAGTATTCTGCATCCCAATCAGATACCACAACCACCTAAACTATGGAtttt                                                                                                                     >  1:513978/1‑251 (MQ=255)                                                                                                                              aaagaaagAACTGAAAGTGGGCGATTTGGTTTTTTTCCGTAGAAATAATCATGTAGGCGTATACGTCGGCAACGATCAGTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGTTCTTTAAGTTTGATTAGATTGAATAAAAAGTATTCTGCATCCCAATCAGATACCACAACCAACTAAACTATGGATTTTTATgg                                                                                                                >  1:215231/1‑251 (MQ=255)                                                                                                                                    agaaCTGAAAGTGGGCGATTTGGTTTTTTTCCGTAGAAATAATCATGTAGGCGTATACGTCGGCAACGATCAGTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAg                                                                                                                                                                                                                                             >  1:865286/1‑120 (MQ=255)                                                                                                                                     gaaCTGAAAGTGGGCGATTTGGTTTTTTTCCGTAGAAATAATCATGTAGGCGTATACGTCGGCAACGATCAGTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATAccc                                                                                                                                                                                                                >  1:277107/1‑148 (MQ=255)                                                                                                                                      aaCTGAAAGTGGGCGATTTGGTTTTTTTCCGTAGAAATAATCATGTAGGCGTATACGTCGGCAACGATCAGTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGTTCTTTAAGTTTGATTAGATTGAATAAAAAGTATTCTGCATCCCAATCAGATACCACAACCAACTAAACTATGGATTTTTATGGTCAAACat                                                                                                        >  1:450364/1‑251 (MQ=255)                                                                                                                                      aaCTGAAAGTGGGCGATTTGGTTTTTTTCCGTAGAAATAATCATGTAGGCGTATACGTCGGCAACGATCAGTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGTTCTTTAAGTTTGATTAGATTGAATAAAAAGTATTCTGCATCCCAATCAGATACCACAACCAACTAAACTATGGATTTTTATGGTCAAACat                                                                                                        >  1:657697/1‑251 (MQ=255)                                                                                                                                           aaaGTGGGCGATTTGGTTTTTTTCCGTAGAAATAATCATGTAGGCGTATACGTCGGCAACGATCAGTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGTTCTTTAAGTTTGATTAGATTGAATAAAAAGTATTCTGCATCCCAATCAGATACCACAACCAACTAAACTATGGATTTTTATGGTCAAACATTATTc                                                                                                   <  1:234727/251‑1 (MQ=255)                                                                                                                                                          gTTTTTTTCCGTAGAAATAATCATGTAGGCGTATACGTCGGCAACGATCAGTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATcc                                                                                                                                                                                                           >  1:620671/1‑132 (MQ=255)                                                                                                                                                          gTTTTTTTCCGTAGAAATAATCATGTAGGCGTATACGTCGGCAACGATCAGTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGTTCTTTAAGTTTGATTAGATTGAATAAAAAGTATTCTGCATCCCAATCAGATACCACAACCAACTAAACTATGGATTTTTATGGTCAAACATTATTCAATCGAATTCAAAca                                                                                    >  1:724202/1‑251 (MQ=255)                                                                                                                                                                       gAAATAATCATGTAGGCGTATACGTCGGCAACGATCAGTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGTTCTTTAAGTTTGATTAGATTGAATAAAAAGTATTCTGCATCCCAATCAGATACCACAACCAACTAAACTATGGATTTTTATGGTCAAACATTATTCAATCGAATTCAAACATCATATCATccg                                                                        >  1:705674/1‑250 (MQ=255)                                                                                                                                                                              tCATGTAGGCGTATACGTCGGCAACGATCAGTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGTTCTTTAAGTTTGATTAGATTGAATAAAAAGTATTCTGCATCCCAATCAGATACCACAACCAACTAAACTATGGATTTTTATGGTCAAACATTATTCAATCGAATTCAAACatcatatca                                                                            <  1:835903/239‑1 (MQ=255)                                                                                                                                                                               cATGTAGGCGTATACGTCGGCAACGATCAGTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGTTCTTTAAGTTTGATTAGATTGAATAAAAAGTATTCTGCATCCCAATCAGATACCACAACCAACTAAACTATGGATTTTTATGGTCAAACATTATTCAATCGAATTCAAACatcatat                                                                              <  1:153652/236‑1 (MQ=255)                                                                                                                                                                                aTGTAGGCGTATACGTCGGCAACGATCAGTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGTTCTTTAAGTTTGATTAGATTGAATAAAAAGTATTCTGc                                                                                                                                                             >  1:226873/1‑156 (MQ=255)                                                                                                                                                                                     ggCGTATACGTCGGCAACGATCAGTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGTTCTTTAAGTTTGATTAGATTGAATAAAAAGTATTCTGCATCCCAATCAGATACCACAACCAACTAAACTATGGATTTTTATGGTCAAACATTATTCAATCGAATTCAAACATCATATCATCCGCCGACGTaagcaag                                                          >  1:765166/1‑250 (MQ=255)                                                                                                                                                                                     ggCGTATACGTCGGCAACGATCAGTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGTTCTTTAAGTTTGATTAGATTGAATAAAAAGTATTCTGCATCCCAATCAGATACCACAACCAACTAAACTATGGATTTTTATGGTCAAACATTATTCAATCGAATTCAAACATCATATCATCCGCCGACGTaagcaag                                                          >  1:376468/1‑250 (MQ=255)                                                                                                                                                                                        gTATACGTCGGCAACGATCAGTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGTTCTTTAAGTTTGATTAGATTGAATAAAAAGTATTCTGCATCCCAATCAGATACCACAACCAACTAAACTATGGATTTTTATGGTCAAACATTATTCAATCGAATTCAAACatcatatc                                                                             >  1:436864/1‑228 (MQ=255)                                                                                                                                                                                        gTATACGTCGGCAACGATCAGTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGTTCTTTAAGTTTGATTAGATTGAATAAAAAGTATTCTGCATCCCAATCAGATACCACAACCAACTAAACTATGGATTTTTATGGTCAAACATTATTCAATCGAATTCAAACATCATATCATCCGCCGACGTAAGCAAGCGGa                                                      >  1:816400/1‑251 (MQ=255)                                                                                                                                                                                                     aCGATCAGTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGTTCTTTAAGTTTGATTAGATTGAATAAAAAg                                                                                                                                                                     <  1:79006/127‑1 (MQ=255)                                                                                                                                                                                                          cAGTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGTTCttt                                                                                                                                                                                              >  1:84469/1‑97 (MQ=255)                                                                                                                                                                                                          cAGTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGTTCTTTAAGTTTGATTAGATTGAATAAAAAGTATTCTGCATCCCAATCAGATACCACAACCAACTAAACTATGGATTTTTATGGTCAAACATTATTCAATCGAATTCAAACATCATATCATCCGCCGACGTaagca                                                            >  1:537407/1‑227 (MQ=255)                                                                                                                                                                                                            gTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGTTCTTTAAGTTTGATTAGATTGAATAAAAAGTATTCTGCATCCCAATCAGATACCACAACCAACTAAACTATGGATTTTTATGGTCAAACATTATTCAATCGAATTCAAACatcatatc                                                                             >  1:317398/1‑208 (MQ=255)                                                                                                                                                                                                            gTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGTTCTTTAAGTTTGATTAGATTGAATAAAAAGTATTCTGCATCCCAATCAGATACCACAACCAACTAAACTATGGATTTTTATGGTCAAACATTATTCAATCGAATTCAAACATCATATCATCCGCCGACGTAAGCAAGCGGAAAAAAACGGTCATATTg                                     >  1:236526/1‑248 (MQ=255)                                                                                                                                                                                                            gTTTATGCATTCCAGTACAAGCCGAGGCGTGATTACTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGCTCTTTAAGTTTGATTAGATTGAATAAAAAGTATTCTGCATCCCAATCAGATACCACAACCAAATAAACTATGGATTTTTATGGTCAAACATTATTCAATCGAATTCAAACATCATATCATCCGCCGACGTAAGCAAGCGGAAAAAAACGGTCATATTGCGt                                  >  1:899683/1‑251 (MQ=255)                                                                                                                                                                                                                aTGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGTTCTTTAAGTTTGATTAGATTGAATAAAAAGTATTCTGCATCCCAATCAGATACCACAACCAACTAAACTATGGATTTTTATGGTCAAACATTATTCAATCGAATTCAAACATCATATCATCCGCCGACGTAAGCAAGCGGAAAAAAACGGTCATATTGCGTCaaa                              >  1:773468/1‑251 (MQ=255)                                                                                                                                                                                                                     ttCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGTTCTTTAAGTTTGATTAGAt                                                                                                                                                                                >  1:769906/1‑100 (MQ=255)                                                                                                                                                                                                                     ttCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGTTCTTTAAGTTTGATTAGATTGAATAAAAAGTATTCTGCATCCCAATCAGATACCAcaac                                                                                                                                        <  1:774427/140‑1 (MQ=255)                                                                                                                                                                                                                     ttCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGTTCTTTAAGTTTGATTAGATTGAATAAAAAGTATTCTGCATCCCAATCAGATACCACAACCAACTAAACTATGGATTTTTATGGTCAAACATTATTCAATCGAATTCAAACatcatatc                                                                             >  1:353588/1‑199 (MQ=255)                                                                                                                                                                                                                     ttCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGTTCTTTAAGTTTGATTAGATTGAATAAAAAGTATTCTGCATCCCAATCAGATACCACAACCAACTAAACTATGGATTTTTATGGTCAAACATTATTCAATCGAATTCAAACATCATATCATCCGCCGACGTAAGCAAGCGGAAAAAAACGGTCATATTGCGTCAAACTTa                          >  1:94844/1‑250 (MQ=255)                                                                                                                                                                                                                     ttCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGTTCTTTAAGTTTGATTAGATTGAATAAAAAGTATTCTGCATCCCAATCAGATACCACAACCAACTAAACTATGGATTTTTATGGTCAAACATTATTCAATCGAATTCAAACATCATATCATCCGCCGACGTAAGCAAGCGGAAAAAAACGGTCATATTGCGTCAAACTTa                          >  1:72292/1‑250 (MQ=255)                                                                                                                                                                                                                     ttCCAGTACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGTTCTTTAAGTTTGATTAGATTGAATAAAAAGTATTCTGCATCCCAATCAGATACCACAACCAACTAAACTATGGATTTTTATGGTCAAACATTATTCAATCGAATTCAAACATCATATCATCCGCCGACGTAAGCAAGCGGAAAAAAACGGTCATATTGCGTCAAACTTa                          >  1:694168/1‑250 (MQ=255)                                                                                                                                                                                                                           tACAAGCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGTTCTTTAAGTTTGATTAGATTGAATAAAAAGTATTCTGCATCCCAATCAGATACCACAACCAACTAAACTATGGATTTTTATGGTCAAACATTATTCAATCGAATTCAAACATCATATCATCCGCCGACGTAAGCAAGCGGAAAAAAACGGTCATATTGCGTCAAACTTACTCAc                     <  1:502011/249‑1 (MQ=255)                                                                                                                                                                                                                                gCCGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGTTCTTTAAGTTTGATTAGATTGAATAAAAAGTATTCTGCATCCCAATCAGATACCACAACCAACTAAACTATGGATTTTTATGGTCAAACATTATTCAATCGAATTCAAACATCATATCATCCGCCGACGTAAGCAAGCGGAAAAAAACGGTCATATTGCGTCAAACTTACTCACGTGCAAt              >  1:274980/1‑251 (MQ=255)                                                                                                                                                                                                                                 ccGAGGCGTGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGTTCTTTAAGTTTGATTAGATTGAATAAAAAGTATTCTGCATCCCAATCAGATACCACAACCAACTAAACTATGGATTTTTATGGTCAAACATTATTCAATCGAATTCAAACATCATATCATCCGCCGACGTAAGCAAGCGGAAAAAAACGGTCATATTGCGTCAAACTTACTCACGTGCaa               <  1:777167/249‑1 (MQ=255)                                                                                                                                                                                                                                         tGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGTTCTTTAAGTTTGATTAGATTGAATAAAAAGTATTCTGCATCCCAATCAGATACCACAACCAACTAAACTATGGATTTTTATGGTCAAACATTATTCAATCGAATTCAAACATCATATCATCCGCCGACGTAAGCAAGCGGAAAAAAACGGTCATATTGCGTCAAACTTACTCACGTGCAATCTGATTAc      >  1:843269/1‑250 (MQ=255)                                                                                                                                                                                                                                         tGATTATTGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGTTCTTTAAGATTGATTAGATTGAATAAAAAGTATTCTGCATCCCAATCAGATACCACAACCAACTAAACTATGGATTTTTATGGTCAAACATTATTCAATCGAATTCAAACatcatatca                                                                            >  1:267213/1‑180 (MQ=255)                                                                                                                                                                                                                                            ttattGAGTCCTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGTTCTTTAAGTTTGATTAGATTGAATAAAAAGTATTCTGCATCCCAATCAGATACCACAACCAACTAAACTATGGATTTTTATGGTCAAACATTATTCAATCGAATTCAAACATCATATCATCCGCCGACGTAAGCAAGCGGAAAAAAACGGTCATATTGCGTCAAACTTACTCACGTGCAATCTGATTACCAGa  <  1:337925/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 TTTGGCGGCACCACAAAAAATGGCATTGATTGTTCCGGCTTTATGCAAACCACCTTTTTAGATGCGTTTGGTGTGACATTGCCGCGTTCGACTTCCGAACAGCGTTATGTCGGTAAAGCGATTCAAAAGAAAGAACTGAAAGTGGGCGATTTGGTTTTTTTCCGTAGAAATAATCATGTAGGCGTATACGTCGGCAACGATCAGTTTATGCATTCCAGTACAAGCCGAGGCGTGATTATTGAGTCTTTAAGCGACAATTACTGGGCGAGAACGTATACCCAATCCCGTCGGGTTCTTTAAGTTTGATTAGATTGAATAAAAAGTATTCTGCATCCCAATCAGATACCACAACCAACTAAACTATGGATTTTTATGGTCAAACATTATTCAATCGAATTCAAACATCATATCATCCGCCGACGTAAGCAAGCGGAAAAAAACGGTCATATTGCGTCAAACTTACTCACGTGCAATCTGATTACCAGA  >  1/1022581‑1023066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑