Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	14,038	C→A	intergenic (–/+117096)	– / ← unknown	–/tRNA‑Ala‑GGC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	14,038	0	C	A	100.0%	183.1 / NA	67	intergenic (–/+117096)	–/unknown	–/tRNA‑Ala‑GGC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (27/40);  total (27/40)

GTCATTTTTTCAAGCGTTTTTATCATTAAAAAACTATTTTAAAAACCACCGCACTTTTAATAATGCACCATGGGGGCCCCATCTGACCCGCCTGAGCGACTTGTAATTTTTAGGAAATTTTCGCTTTGTTTGAGCTGCGTAGTGGCGAGTTCAAAAATTTCCGTTAAAAAAATTGCAACCAAGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGCAACAGCTAAACAAAAAGCGTACATTATACGCCTTTATCAATAATAAAAAACTAACCTACGCAACGGTCAATTGTCCGCCGTACAAAATAAAATATCGCAGACACAACACGCCGATTAAATCAAATATAGATACCAGAATGATGAAATGACGATTGTATCTTAATTTATCGCTAACCAATAGATTACCGGCGAGCGGGATGAGGATGCCGATAAGCATGACGCCGAT  >  1/13792‑14264                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                   gTCATTTTTTCAAGCGTTTTTATCATTAAAAAACTATTTTAAAAACCACCGCACTTTTAATAATGCACCATGGGGGCCCCATCTGACCCGCCTGAGCGACTTGTAATTTTTAGGAAATTTTCGCTTTGTTTGAGCTGCGTAGTGGCGAGTTCAAAAATTTCCGTTAAAAAAATTGCAACCAAGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACa                                                                                                                                                                                                                                >  1:680420/1‑251 (MQ=255)   cATTTTTTCAAGCGTTTTTATCATTAAAAAACTATTTTAAAAACCACCGCACTTTTAATAATGCACCATGGGGGCCCCATCGGACCCGCCTGAGCGACTTGTAATTTTTAGGAAATTTTCGCTTTGTTTGAGCTGCGTAGTGGCGAGTTCAAAAATTTCCGTTAAAAAAATTGCAACCAAGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGc                                                                                                                                                                                                                              <  1:442663/251‑1 (MQ=255)                 tttttATCATTAAAAAACTATTTTAAAAACCACCGCACTTTTAATAATGCACCATGGGGGCCCCATCTGACCCGCCTGAGCGACTTGTAATTTTTAGGAAATTTTCGCTTTGTTTGAGCTGCGTAGTGGCGAGTTCAAAAATTTCCGTTAAAAAAATTGCAACCAAGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGt                                                                                                                                                                                                                >  1:574394/1‑251 (MQ=255)                        cATTAAAAAACTATTTTAAAAACCACCGCACTTTTAATAATGCACCATGGGGGCCCCATCTGACCCGCCTGAGCGACTTGTAATTTTTAGGAAATTTTCGCTTTGTTTGAGCTGCGTAGTGGCGAGTTCAAAAATTTCCGTTAAAAAAATTGCAACCAAGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAATAGAAACAGCTAAACAAAAAGCGTACATtat                                                                                                                                                                                                         <  1:677168/251‑1 (MQ=255)                           tAAAAAACTATTTTAAAAACCACCGCACTTTTAATAATGCACCATGGGGGCCCCATCTGACCCGCCTGAGCGACTTGTAATGTTTAGGAAATTTTCGCTTTGTTTGAGCTGCGTAGTGGCGAGTTCAAAAATTTCCGTTAAAAAAATTGCAACCAAGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACATTATACg                                                                                                                                                                                                      <  1:441252/251‑1 (MQ=255)                             aaaaaCTATTTTAAAAACCACCGCACTTTTAATAATGCACCATGGGGGCCCCATCTGACCCGCCTGAGCGACTTGTAATTTTTAGGAAATTTTCGCTTTGTTTGAGCTGCGTAGTGGCGAGTTCAAAAATTTCCGTTAAAAAAATTGCAACCAAGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACATTATACGcc                                                                                                                                                                                                    <  1:583970/251‑1 (MQ=255)                             aaaaaCTATTTTAAAAACCACCGCACTTTTAATAATGCACCATGGGGGCCCCATCTGACCCGCCTGAGCGACTTGTAATTTTTAGGAAATTTTCGCTTTGTTTGAGCTGCGTAGTGGCGAGTTCAAAAATTTCCGTTAAAAAAATTGCAACCAAGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACATTATACGcc                                                                                                                                                                                                    <  1:696707/251‑1 (MQ=255)                                  cTATTTTAAAAACCACCGCACTTTTAATAATGCACCATGGGGGCCCCATCTGACCCGCCTGAGCGACTTGTAATTTTTAGGAAATTTTCGCTTTGTTTGAGCTGCGTAGTGGCGAGTTCAAAAATTTCCGTTAAAAAAATTGCAACCAAGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACATTATACGCCTTTAt                                                                                                                                                                                               <  1:822463/251‑1 (MQ=255)                                  cTATTTTAAAAACCACCGCACTTTTAATAATGCACCATGGGGGCCCCATCTGACCCGCCTGAGCGACTTGTAATTTTTAGGAAATTTTCGCTTTGTTTGAGCTGCGTAGTGGCGAGTTCAAAAATTTCCGTTAAAAAAATTGCAACCAAGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACATTATACGCCTTTAt                                                                                                                                                                                               <  1:890926/251‑1 (MQ=255)                                   tATTTTAAAAACCACCGCACTTTTAATAATGCACCATGGGGGCCCCATCTGACCCGCCTGAGCGACTTGTAATTTTTAGGAAATTTTCGCTTTGTTTGAGCTGCGTAGTGGCGAGTTCAAAAATTTCCGTTAAAAAAATTGCAACCAAGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACATTATACGCCTTTATc                                                                                                                                                                                              <  1:690344/251‑1 (MQ=255)                                    aTTTTAAAAACCACCGCACTTTTAATAATGCACCATGGGGGCCCCATCTGACCCGCCTGAGCGACTTGTAATTTTTAGGAAATTTTCGCTTTGTTTGAGCTGCGTAGTGGCGAGTTCAAAAATTTCCGTTAAAAAAATTGCAACCAAGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACATTATACGcc                                                                                                                                                                                                    <  1:84119/244‑1 (MQ=255)                                    aTTTTAAAAACCACCGCACTTTTAATAATGCACCATGGGGGCCCCATCTGACCCGCCTGAGCGACTTGTAATTTTTAGGAAATTTTCGCTTTGTTTGAGCTGCGTAGTGGCGAGTTCAAAAATTTCCGTTAAAAAAATTGCAACCAAGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACATTATACGCCTTTATCa                                                                                                                                                                                             >  1:672118/1‑251 (MQ=255)                                                accGCACTTTTAATAATGCACCATGGGGGCCCCATCTGACCCGCCTGAGCGACTTGTAATTTTTAGGAAATTTTCGCTTTGTTTGAGCTGCGTAGTGGCGAGTTCAAAAATTTCCGTTAAAAAAATTGCAACCAAGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACATTATACGCCTTTATCAATAATAAAAAAc                                                                                                                                                                                 <  1:703894/251‑1 (MQ=255)                                                    cACTTTTAATAATGCACCATGGGGGCCCCATCTGACCCGCCTGAGCGACTTGTAATTTTTAGGAAATTTTCGCTTTGTTTGAGCTGCGTAGTGGCGAGTTCAAAAATTTCCGTTAAAAAAATTGCAACCAAGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACATTATACGc                                                                                                                                                                                                     <  1:492407/227‑1 (MQ=255)                                                      cTTTTAATAATGCACCATGGGGGCCCCATCTGACCCGCCTGAGCGACTTGTAATTTTTAGGAAATTTTCGCTTTGTTTGAGCTGCGTAGTGGCGAGTTCAAAAATTTCCGTTAAAAAAATTGCAACCAAGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACaa                                                                                                                                                                                                                       <  1:883793/207‑1 (MQ=255)                                                      cTTTTAATAATGCACCATGGGGGCCCCATCTGACCCGCCTGAGCGACTTGTAATTTTTAGGAAATTTTCGCTTTGTTTGAGCTGCGTAGTGGCGAGTTCAAAAATTTCCGTTAAAAAAATTGCAACCAAGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACAt                                                                                                                                                                                                            <  1:582330/218‑1 (MQ=255)                                                      cTTTTAATAATGCACCATGGGGGCCCCATCTGACCCGCCTGAGCGACTTGTAATTTTTAGGAAATTTTCGCTTTGTTTGAGCTGCGTAGTGGCGAGTTCAAAAATTTCCGTTAAAAAAATTGCAACCAAGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACATTATACGCCTTTATc                                                                                                                                                                                              <  1:300381/232‑1 (MQ=255)                                                         ttAATAATGCACCATGGGGGCCCCATCTGACCCGCCTGAGCGACTTGTAATTTTTAGGAAATTTTCGCTTTGTTTGAGCTGCGTAGTGGCGAGTTCAAAAATTTCCGTTAAAAAAATTGCAACCAAGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACATTATACGCCTTTATCAATAATAAAAAACTAACCTACg                                                                                                                                                                        >  1:749278/1‑251 (MQ=255)                                                          taataaTGCACCATGGGGGCCCCATCTGACCCGCCTGAGCGACTTGTAATTTTTAGGAAATTTTCGCTTTGTTTGAGCTGCGTAGTGGCGAGTTCAAAAATTTCCGTTAAAAAAATTGCAACCAAGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAAc                                                                                                                                                                                                                         >  1:298305/1‑201 (MQ=255)                                                            ataatGCACCATGGGGGCCCCATCTGACCCGCCTGAGCGACTTGTAATTTTTAGGAAATTTTCGCTTTGTTTGAGCTGCGTAGTGGCGAGTTCAAAAATTTCCGTTAAAAAAATTGCAACCAAGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCg                                                                                                                                                                                                                 >  1:896176/1‑207 (MQ=255)                                                             taatGCACCATGGGGGCCCCATCTGACCCGCCTGAGCGACTTGTAATTTTTAGGAAATTTTCGCTTTGTTTGAGCTGCGTAGTGGCGAGTTCAAAAATTTCCGTTAAAAAAATTGCAACCAAGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACATTATACGCCTTTATCAATAATAAAAAACTAACCTACGCAAc                                                                                                                                                                    <  1:516985/251‑1 (MQ=255)                                                               atGCACCATGGGGGCCCCATCTGACCCGCCTGAGCGACTTGTAATTTTTAGGAAATTTTCGCTTTGTTTGAGCTGCGTAGTGGCGAGTTCAAAAATTTCCGTTAAAAAAATTGCAACCAAGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACATTATACGCCTTTATCAATAATAAAAAAc                                                                                                                                                                                 <  1:135693/236‑1 (MQ=255)                                                                tGCACCATGGGGGCCCCATCTGACCCGCCTGAGCGACTTGTAATTTTTAGGAAATTTTCGCTTTGTTTGAGCTGCGTAGTGGCGAGTTCAAAAATTTCCGTTAAAAAAATTGCAACCAAGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACATTATACGCCtt                                                                                                                                                                                                  <  1:276821/218‑1 (MQ=255)                                                                    ccATGGGGGCCCCATCTGACCCGCCTGAGCGACTTGTAATTTTTAGGAAATTTTCGCTTTGTTTGAGCTGCGTAGTGGCGAGTTCAAAAATTTCCGTTAAAAAAATTGCAACCAAGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACATTATACGcc                                                                                                                                                                                                    >  1:602702/1‑212 (MQ=255)                                                                    ccATGGGGGCCCCATCTGACCCGCCTGAGCGACTTGTAATTTTTAGGAAATTTTCGCTTTGTTTGAGCTGCGTAGTGGCGAGTTCAAAAATTTCCGTTAAAAAAATTGCAACCAAGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACATTATACGc                                                                                                                                                                                                     <  1:124116/211‑1 (MQ=255)                                                                    ccATGGGGGCCCCATCTGACCCGCCTGAGCGACTTGTAATTTTTAGGAAATTTTCGCTTTGTTTGAGCTGCGTAGTGGCGAGTTCAAAAATTTCCGTTAAAAAAATTGCAACCAAGCGAAGATCAGCGGTGAAGCTGGGGCGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACATTATACGCCTTTATCAATAATAAAAAACTAACCTACGCAACGGCCaaa                                                                                                                                                             >  1:21040/1‑250 (MQ=255)                                                                      aTGGGGGCCCCATCTGACCCGCCTGAGCGACTTGTAATTTTTAGGAAATTTTCGCTTTGTTTGAGCTGCGTAGTGGCGAGTTCAAAAATTTCCGTTAAAAAAATTGCAACCAAGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAAc                                                                                                                                                                                                                         >  1:134523/1‑189 (MQ=255)                                                                       tGGGGGCCCCATCTGACCCGCCTGAGCGACTTGTAATTTTTAGGAAATTTTCGCTTTGTTTGAGCTGCGTAGTGGCGAGTTCAAAAATTTCCGTTAAAAAAATTGCAACCAAGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACATTATACGCCTTTATCAATAATAAAAAACTAACCTACGCAACGGTCAATTGt                                                                                                                                                          >  1:865275/1‑251 (MQ=255)                                                                                cATCTGACCCGCCTGAGCGACTTGTAATTTTTAGGAAATTTTCGCTTTGTTTGAGCTGCGTAGTGGCGAGTTCAAAAATTTCCGTTAAAAAAATTGCAACCAAGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAAc                                                                                                                                                                                                                         <  1:848181/179‑1 (MQ=255)                                                                                cATCTGACCCGCCTGAGCGACTTGTAATTTTTAGGAAATTTTCGCTTTGTTTGAGCTGCGTAGTGGCGAGTTCAAAAATTTCCGTTAAAAAAATTGCAACCAAGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACATTATACGCCTTTATCAATAATAAAAAACTAACCTACGCAACGGTCAATTGTCCGCCGTa                                                                                                                                                  >  1:518942/1‑250 (MQ=255)                                                                                      aCCCGCCTGAGCGACTTGTAATTTTTAGGAAATTTTCGCTTTGTTTGAGCTGCGTAGTGGCGAGTTCAAAAATTTCCGTTAAAAAAATTGCAACCAAGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACATTATACGCCTTTATCAATAATAAAAAACTAACCTACGCAACGGTCAATTGTCCGCCGTACaaaat                                                                                                                                            <  1:470612/250‑1 (MQ=255)                                                                                                             tttAGGAAATTTTCGCTTTGTTTGAGCTGCGTAGTGGCGAGTTCAAAAATTTCCGTTAAAAAAATTGCAACCAAGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACATTATACGCCTTTATCAATAATAAAAAACTAACCTACGCAACGGTCAATTGTCCGCCGTACAAAATAAAATATCGCAGACACAACACGcc                                                                                                                    <  1:139623/251‑1 (MQ=255)                                                                                                                      ttttCGCTTTGTTTGAGCTGCGTAGTGGCGAGTTCAAAAATTTCCGTTAAAAAAATTGCAACCAAGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACATTATACGCCTTTATCAATAATAAAAAACTAACCTACGCAACGGTCAATTGTCCGCCGTACAAAATAAAATATCGCAGACACAACACGCCGATTAAATc                                                                                                           <  1:153618/251‑1 (MQ=255)                                                                                                                            cTTTGTTTGAGCTGCGTAGTGGCGAGTTCAAAAATTTCCGTTAAAAAAATTGCAACCAAGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACATTATACGCCTTTATCAATAATAAAAAACTAACCTACGCAACGGTCAATTGTCCGCCGTACAAAATAAAATATCGCAGACACAACACGCCGATTAAATCAAatat                                                                                                     <  1:593557/251‑1 (MQ=255)                                                                                                                            cTTTGTTTGAGCTGCGTAGTGGCGAGTTCAAAAATTTCCGTTAAAAAAATTGCAACCAAGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACATTATACGCCTTTATCAATAATAAAAAACTAACCTACGCAACGGTCAATTGTCCGCCGTACAAAATAAAATATCGCAGACACAACACGCCGATTAAATCAAata                                                                                                      <  1:588985/250‑1 (MQ=255)                                                                                                                              ttgtttgAGCTGCGTAGTGGCGAGTTCAAAAATTTCCGTTAAAAAAATTGCAACCAAGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAAc                                                                                                                                                                                                                         <  1:749844/133‑1 (MQ=255)                                                                                                                               tgtttgAGCTGCGTAGTGGCGAGTTCAAAAATTTCCGTTAAAAAAATTGCAACCAAGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACATTATACGCCTTTATCAATAATAAAAAACTAACCTACGCAACGGTCAATTGTCCGCCGTACAAAATAAAATATCGCAGACACAACACGCCGATTAAATCAAATATAGa                                                                                                  >  1:358833/1‑251 (MQ=255)                                                                                                                                gtttgAGCTGCGTAGTGGCGAGTTCAAAAATTTCCGTTAAAAAAATTGCAACCAAGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACaa                                                                                                                                                                                                                       >  1:693501/1‑133 (MQ=255)                                                                                                                                    gAGCTGCGTAGTGGCGAGTTCAAAAATTTCCGTTAAAAAAATTGCAACCAAGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACATTATACGCCTTTATCAATAATAAAAAACTAACCTACGCAACGGTCAATTGTCCGCCGTACAAAATAAAATATCGCAGACACAACACGCCGATTAAATCAAATATAGATACCa                                                                                             >  1:81419/1‑251 (MQ=255)                                                                                                                                       cTGCGTAGTGGCGAGTTCAAAAATTTCCGTTAAAAAAATTGCAACCAAGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACATTATACGCCTTTATCAATAATAAAAAACTAACCTACGCAACGGTCAATTGTCCGCCGTACAAAATAAAATATCGCAGACACAACACGCCGATTAAATCAAATATAGATACCAGaa                                                                                          <  1:53615/251‑1 (MQ=255)                                                                                                                                         gCGTAGTGGCGAGTTCAAAAATTTCCGTTAAAAAAATTGCAACCAAGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACATTATACGCCTTTATCAATAATAAAAAACTAACCTACGCAACGGTCAATTGTCCGCCGTACaaaataaaat                                                                                                                                       <  1:764891/204‑1 (MQ=255)                                                                                                                                         gCGTAGTGGCGAGTTCAAAAATTTCCGTTAAAAAAATTGCAACCAAGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACATTATACGCCTTTATCAATAATAAAAAACTAACCTACGCAACGGTCAATTGTCCGCCGTACAAAATAAAATATCGCAGACACAACACGCCGATTAAATCAAATATAGATACCAGAatg                                                                                        >  1:714076/1‑251 (MQ=255)                                                                                                                                           gTAGTGGCGAGTTCAAAAATTTCCGTTAAAAAAATTGCAACCAAGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACAt                                                                                                                                                                                                            >  1:787117/1‑133 (MQ=255)                                                                                                                                                     gTTCAAAAATTTCCGTTAAAAAAATTGCAACCAAGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACATTATACGCCTTTATCAATAATAAAAAACTAACCTACGCAACGGTCAATTGTCCGCCGTACAAAATAAAATATCGCAGACACAACACGCCGATTAAATCAAATATAGATACCAGAATGATGaaa                                                                                  <  1:216962/245‑1 (MQ=255)                                                                                                                                                                      aaaaaaaTTGCAACCAAGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACATTATACg                                                                                                                                                                                                      >  1:459322/1‑112 (MQ=255)                                                                                                                                                                            aTTGCAACCAAGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACATTATACGCCTTTATCAATAATAAAAAACTAACCTACGCAACGGTCAATTGTCCGCCGTACAAAATAAAATATCGCAGACACAACACGCCGATTAAATCAAATATAGAt                                                                                                 <  1:506618/207‑1 (MQ=255)                                                                                                                                                                             ttGCAACCAAGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACATTATACGCCTTTATCAATAATAAAAAACTAACCTACGCAACGGTCAATTGTCCGCCGTACAAAATAAAATATCGCAGACACAACACGCCGATTAAATCAAATATAGATACCAGAATGATGAAATGACGATTGTATCTTAATTTATCGCTAAc                                                     >  1:517600/1‑250 (MQ=255)                                                                                                                                                                                    cAAGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACATTATACGCCTTTATCAATAATAAAAAACTAACCTACGCAACGGTCAATTGTCCGCCGTACAAAATAAAATATCGCAGACACAACACGCCGATTAAATCAAATATAGATACCAGAATGATGAAATGACGATTGTATCTTAATTTATCGCTAACCAATAGAt                                             <  1:297165/251‑1 (MQ=255)                                                                                                                                                                                     aaGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACATTATACGCCTTTATCAATAATAAAAAACTAACCTACGCAACGGTCAATTGTCCGCCGTACAAAATAAAATATCGCAGACACAACACGCCGATTAAATCAAATATAGATACCAGAATGATGAAATGACGATTGTATCTTAATTTATCGCTAACCAATAg                                               <  1:660119/248‑1 (MQ=255)                                                                                                                                                                                      aGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACATTATACGCCTTTATCAATAATAAAAAACTAACCTACGCAACGGTCAATTGTCCGCCGTACAAAATAAAATATCGCAGACACAACACGCCGATTAAATCAAATATAGATACCAGAATGATGAAATGACGATTGTATCTTAATTTATCGc                                                         >  1:747367/1‑237 (MQ=255)                                                                                                                                                                                       gCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACATTATACGCCTTTATCAATAATAAAAAACTAACCTACGCAACGGTCAATTGTCCGCCGTACAAAATAAAATATCGCAGACACAACACGCCGATTAAATCAAATATAGAt                                                                                                 >  1:704889/1‑196 (MQ=255)                                                                                                                                                                                        cGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACATTATACGCCTTTATCAATAATAAAAAACTAACCTACGCAACGGTCAATTGTCCGCCGTACAAAATAAAATATCGCAGACACAACACGCCGATTAAATCAAATATAGATACCAGAATGATGAAATGACGATTGTATCTTAATTTATCGCTAACCAATAGATTAc                                          <  1:290127/250‑1 (MQ=255)                                                                                                                                                                                                  cGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACATTATACGCCTTTATCAATAATAAAAAACTAACCTACGCAACGGTCAATTg                                                                                                                                                           >  1:769714/1‑127 (MQ=255)                                                                                                                                                                                                    gTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACATTATACGCCTTTATCAATAATAAAAAACTAACCTACGCAACGGTCAATTGTCCGCCGTACAAAATAAAATATCGCAGACACAACACGCCGATTAAATCAAATATAGATACCAGAATGATGAAATGACGATTGTATCTTAATTTATCGCTAACCAATAGATTACCGGCGAGCGGGAt                             >  1:592193/1‑251 (MQ=255)                                                                                                                                                                                                       aaGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACAtt                                                                                                                                                                                                           <  1:465463/74‑1 (MQ=255)                                                                                                                                                                                                          cTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACATTATACGCCTTTATCAATAATAAAAAACTAACCTACGCAACGGTCAATTGTCCGCCGTACAAAATAAAATATCGCAGACACAACACGCCGATTAAATCAAATATAGATACCAGAATGATGAAATGACGATTGTATCTTAATTTATCGCTAACCAATAGATTACCGGCGAGCGGGATGAGGa                        <  1:778179/250‑1 (MQ=255)                                                                                                                                                                                                            ggggTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACaa                                                                                                                                                                                                                       >  1:122669/1‑57 (MQ=255)                                                                                                                                                                                                              ggTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACATTATACGCCTTTATCAATAATAAAAAACTAACCTACGCAACGGTCAATTGTCCGCCGTACAAAATAAAATATCGCAGACACAACACGCCGATTAAATCAAATATAGATACCAGAATGATGAAATGACGATTGTATCTTAATTTATCGCTAACCAATAGATTACCGGCGAGCGGGATGAGGATGCCg                   >  1:756960/1‑251 (MQ=255)                                                                                                                                                                                                                   gtTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACATTATACGCCTTTATCAATAATAAAAAACTAACCTACGCAACGGTCAATTGTCCGCCGTACAAAATAAAATATCGCAGACACAACACGCCGATTAAATCAAATATAGATACCAGAATGATGAAATGACGATTGTATCTTAATTTATCGCTAACCAATAGATTACCGGCGAGCGGGATGAGGATGCCGATAAg              >  1:497075/1‑251 (MQ=255)                                                                                                                                                                                                                    ttCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACATTATACGCCTTTATCAATAATAAAAAACTAACCTACGCAACGGTCAATTGTCCGCCGTACAAAATAAAATATCGCAGACACAACACGCCGATTAAATCAAATATAGATACCAGAATGATGAAATGACGATTGTATCTTAATTTATCGCTAACCAATAGATTACCGGCGAGCGGGATGAGGATGCCGATAAGc             <  1:367768/251‑1 (MQ=255)                                                                                                                                                                                                                             aCTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACATTATACGCCTTTATCAATAATAAAAAACTAACCTACGCaa                                                                                                                                                                     <  1:801629/90‑1 (MQ=255)                                                                                                                                                                                                                              cTTTCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACATTATACGCCTTTATCAATAATAAAAAACTAACCTACGCAACGGTCAATTGTCCGCCGTACAAAATAAAATATCGCAGACACAACACGCCGATTAAATCAAATATAGATACCAg                                                                                            <  1:401301/162‑1 (MQ=255)                                                                                                                                                                                                                                ttCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACATTATACGCCTTTATCAATAATAAAAAACTAACCTACGCAACGGTCAATTGTCCGCCGTACAAAATAAAATATCGCAGACACAACACGCCGATTAAATCAAATATAGAt                                                                                                 >  1:593296/1‑155 (MQ=255)                                                                                                                                                                                                                                ttCTTGCACAAGCAAGAAAGTAGAAACAGCTAAACAAAAAGCGTACATTATACGCCTTTATCAATAATAAAAAACTAACCTACGCAACGGTCAATTGTCCGCCGTACAAAATAAAATATCGCAGACACAACACGCCGATTAAATCAAATATAGATACCAGAATGATGAAATGACGATTGTATCTTAATTTATCGCTAACCAATAGATTACCGGCGAGCGGGATGAGGATGCCGATAAGCATGACGCCGAt  >  1:769204/1‑250 (MQ=255)                                                                                                                                                                                                                                          agcaagAAAGTAGAAACAGCTAAACAAAAAGCGTACATTATACGCCTTTATCAATAATAAAAAACTAACCTACGCAACGGTCAATTGTCCGCCGTACAAAATAAAATATCGCAGACACAACACGCCGATTAAATCAAATATAGATACCAGAAtgatga                                                                                    <  1:656483/158‑1 (MQ=255)                                                                                                                                                                                                                                                aaaGTAGAAACAGCTAAACAAAAAGCGTACATTATACGCCTTTATCAATAATAAAAAACTAACCTACGCAACGGTCAATTGTCCGCCGTACAAAATAAAATATCGCAGACACAACACGc                                                                                                                     <  1:535840/119‑1 (MQ=255)                                                                                                                                                                                                                                                  aGTAGAAACAGCTAAACAAAAAGCGTACATTATACGCCTTTATCAATAATAAAAAACTAACCTACGCAACGGTCAATTGTCCGCCGTACAAAATAAAATATCGCAGACACAACACGCCGATTAAATCAAATATAGATACCAGAATGATGAAATGACGAt                                                                           <  1:633162/159‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                   GTCATTTTTTCAAGCGTTTTTATCATTAAAAAACTATTTTAAAAACCACCGCACTTTTAATAATGCACCATGGGGGCCCCATCTGACCCGCCTGAGCGACTTGTAATTTTTAGGAAATTTTCGCTTTGTTTGAGCTGCGTAGTGGCGAGTTCAAAAATTTCCGTTAAAAAAATTGCAACCAAGCGAAGATCAGCGGTGAAGCTGGGGTGTGTTCTTTGCTACTTTCTTGCACAAGCAAGAAAGTAGCAACAGCTAAACAAAAAGCGTACATTATACGCCTTTATCAATAATAAAAAACTAACCTACGCAACGGTCAATTGTCCGCCGTACAAAATAAAATATCGCAGACACAACACGCCGATTAAATCAAATATAGATACCAGAATGATGAAATGACGATTGTATCTTAATTTATCGCTAACCAATAGATTACCGGCGAGCGGGATGAGGATGCCGATAAGCATGACGCCGAT  >  1/13792‑14264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑