Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,000,667	A→G	intergenic (+50093/+5498)	unknown → / ← unknown	tRNA‑Leu‑CAG/tRNA‑Lys‑TTT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,000,667	0	A	G	100.0%	73.0 / NA	27	intergenic (+50093/+5498)	unknown/unknown	tRNA‑Leu‑CAG/tRNA‑Lys‑TTT
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/19);  total (8/19)

ATGTAATGAAAGATTTGCGCAAGGCATTGAAGCGATGAAACAAGACTACATTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCAGAATATATTTACACACAAAGCCAAAATAAGGAAGTAGCTGACAAATTCATTGAGGAAATAGAGTGTCTATCAGAAAAACTTAGTTATATTGCTGATGCCTATACCGACGGGAAGTTTCATATTTTCCCTCTTAAAAATGGGCATTCAGTAAAGTTTCTTGTTGTTGGAACCTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATCATTAAAGGAGAAAGTGTGTATCAT  >  1/1000421‑1000752                                                                                                                                                                                                                                                       |                                                                                      aggTAATGAAAGATTTGCGCAAGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAg                                                                                    <  1:857298/248‑1 (MQ=255)                      aGGCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGaatgtata                                                              >  1:665540/1‑245 (MQ=255)                        gCATTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGaatgtatat                                                             <  1:623153/250‑8 (MQ=255)                          aTTGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTAtattat                                                          <  1:491142/251‑1 (MQ=255)                            tGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGa                                                        <  1:400564/251‑1 (MQ=255)                            tGAAACGATGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGa                                                        <  1:744739/251‑1 (MQ=255)                                   aTGAAACAGGATTACGTTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACg                                                  >  1:657619/1‑250 (MQ=255)                                           ggattacgttGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTAtattat                                                          <  1:39444/226‑1 (MQ=255)                                            gattacgttGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTG‑CGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCa                                       >  1:193324/8‑251 (MQ=255)                                                acgttGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTAtatta                                                           <  1:592328/225‑1 (MQ=255)                                                          aTGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATc                          <  1:93247/251‑1 (MQ=255)                                                          aTGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATc                          <  1:629036/251‑1 (MQ=255)                                                            ggTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATCAt                        >  1:425820/1‑251 (MQ=255)                                                            ggTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATCAt                        >  1:87745/1‑251 (MQ=255)                                                             gTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGt                                                                                   <  1:518623/191‑1 (MQ=255)                                                                 gAAATGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATg                                                         <  1:792671/213‑1 (MQ=255)                                                                  aaaTGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATCATTagga                   <  1:545586/250‑4 (MQ=255)                                                                  aaaTGGCTAGATTTCAGCTTTTAGATAAAGCGGAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATCATTagga                   <  1:604529/250‑4 (MQ=255)                                                                                               gcggAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTAtatta                                                           >  1:269537/4‑181 (MQ=255)                                                                                                  gAATATATTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATCATTaggaaggggtgtatgttattt  <  1:818686/235‑21 (MQ=255)                                                                                                       tatTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCtggttgtagg                                                                      >  1:488859/1‑159 (MQ=255)                                                                                                       tatTTACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATCATTaggaa                  <  1:225443/214‑5 (MQ=255)                                                                                                         tttACACACAAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAATCTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATCATTagga                   <  1:79663/211‑4 (MQ=255)                                                                                                               cacaAAGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCtggttgtagg                                                                      <  1:767337/154‑4 (MQ=255)                                                                                                                   aaGCCAAAGTAAGGAAGTGGCTGACAAATTCATTGAGGAAATAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATc                          <  1:491282/194‑1 (MQ=255)                                                                                                                                                           aTAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATCATTaggaaggggtgtgtgt       >  1:448188/1‑158 (MQ=255)                                                                                                                                                            tAGAGCGTCTATCAGAAAAACTTAGTTACATTGCTGACGCCTATACTGACGGGAGATTTCACATTTTTCCACTTAAAAATGGGCATTCAGTGAAGTTTCTGGTTGTAGGAATGTATATTATGATTTACGCTTTTTTGCCAAAAgg                                  <  1:43191/145‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                      ATGTAATGAAAGATTTGCGCAAGGCATTGAAGCGATGAAACAAGACTACATTGTTCAATGGTCTGAAATGGCTAGATTTCAGCTTTTAGATAAAGCAGAATATATTTACACACAAAGCCAAAATAAGGAAGTAGCTGACAAATTCATTGAGGAAATAGAGTGTCTATCAGAAAAACTTAGTTATATTGCTGATGCCTATACCGACGGGAAGTTTCATATTTTCCCTCTTAAAAATGGGCATTCAGTAAAGTTTCTTGTTGTTGGAACCTATATTATGATTTACGCTTTTTTGCCAAAAGGTGTGAATCATTAAAGGAGAAAGTGTGTATCAT  >  1/1000421‑1000752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑